• To date, all x-ray crystallographic studies of the Thermus thermophilus 30S subunit ( 23 - 26 ), carried out independently by two groups, have failed to visualize the S1 protein. (pnas.org)
  • Here we use X-ray crystallography to define the impact of streptomycin on the decoding site of the Thermus thermophilus 30S ribosomal subunit in complexes with cognate or near-cognate anticodon stem-loop analogues and messenger RNA. (rcsb.org)
  • Cells bearing a temperature-sensitive allele of NMD3 had decreased levels of 60S subunits at the nonpermissive temperature which resulted in the formation of half-mer polysomes. (asm.org)
  • Cryo-EM reconstruction of the Arx1 particle at 11.9-Å resolution reveals regions of extra density on the pre-60S particle attributed to associated biogenesis factors, confirming the immature state of the nascent subunit. (biomedsearch.com)
  • Herein, we deploy a genetic system to starve cells of an essential ribosomal protein, which results in the accumulation of assembly intermediates that are competent for maturation. (pdbj.org)
  • Cells lacking LTV1 grow slowly and are significantly impaired in mature 40S subunit production. (genetics.org)
  • Our crystal structures display a significant local distortion of 16S ribosomal RNA induced by streptomycin, including the crucial bases A1492 and A1493 that participate directly in codon recognition. (rcsb.org)
  • The structure shows a previously unsuspected ability of the ribosomal decoding centre to accommodate non-canonical base pairs. (rcsb.org)
  • In 1999, Ramakrishnan's laboratory published a 5.5 Angstrom resolution structure of the 30S subunit. (wikipedia.org)
  • We suggest that the proximal consequence of these LTV1 mutations is inhibition of the cytoplasmic maturation of 40S subunits and that nuclear retention of pre-40S subunits is a downstream consequence of the failure to release and recycle critical factors back to the nucleus. (genetics.org)
  • The pre-40S and pre-60S subunits are exported independently of one another, with the pre-40S subunits leaving the nucleus more rapidly than the pre-60S subunits. (genetics.org)
  • The subunit interface, as well as important functional regions such as the peptidyl transferase center and the decoding site are mostly conserved, with some differences observed in the surrounding regions. (wikipedia.org)