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Ribosome Subunits, LargeRibosomal ProteinsRibosomesRibosomal Protein S6RNA, RibosomalRibosome Subunits, Small, EukaryoticMolecular Sequence DataRibosome Subunits, Large, EukaryoticRibosome Subunits, SmallEscherichia coliAmino Acid SequenceRibosome Subunits, Small, BacterialBase SequenceProtein BiosynthesisRibosome SubunitsHaloarcula marismortuiProtein SubunitsRibosome Subunits, Large, BacterialSaccharomyces cerevisiaeNucleic Acid ConformationRNA, Ribosomal, 23SRNA, Ribosomal, 18SMutationMacromolecular SubstancesThermus thermophilusPeptide Chain Initiation, TranslationalProtein BindingRNA, BacterialBinding SitesModels, MolecularRibosomal Protein S6 KinasesPeptide Initiation FactorsRNA, MessengerEukaryotic Initiation Factor-3PolyribosomesRNA, TransferSaccharomyces cerevisiae ProteinsCloning, MolecularBacterial ProteinsRibosome Subunits, Large, ArchaealSequence Homology, Amino AcidCell NucleolusElectrophoresis, Polyacrylamide GelEukaryotic Initiation FactorsMolecular WeightAnemia, Diamond-BlackfanKineticsImidoestersEukaryotic Initiation Factor-1RNA, Ribosomal, 5SRNA-Binding ProteinsRNA, FungalSequence AlignmentRNA, Ribosomal, 5.8SProtein Structure, TertiaryRNA PrecursorsProtein ConformationTranscription, GeneticRNA Processing, Post-TranscriptionalRNA, Transfer, MetEscherichia coli ProteinsGenes, FungalParomomycinRibosomal Protein S6 Kinases, 70-kDaPhosphorylationRNA, Ribosomal, 28SSequence Homology, Nucleic AcidFungal ProteinsRNA, Ribosomal, 16SPeptide Chain Elongation, TranslationalGeobacillus stearothermophilusRecombinant ProteinsRNA, Transfer, Amino AcylRNA, ArchaealGenesPoly UCross-Linking Reagents
RRNAMammalianMitochondrial ribosomal proteinGenesHomologousGeneMRNAEukaryoticRibosomesBiogenesisMitochondriaLarge 60SThermusPolypeptideBindsSequenceEukaryotesInteractionsApproximately 80SpeciesBacterial protein synthesisTertiary structureStructuresFoldsRNAsDecreased protein synthesisAbstractBindHomologsProkaryoticNucleusBacteriostaticComplexSynthaseExternal identifiers for the proteinMechanism of action of protein syntheTranslationStructurallyDistinctProteomicsTetracyclines
RRNA15
  • The core of the 60S subunit is formed by the 28S ribosomal RNA (abbreviated 28S rRNA), which is homologous to the prokaryotic 23S rRNA, which also contributes the active site (peptidyl transferase center, PTC) of the ribosome. (wikipedia.org)
  • The rRNA core is decorated with dozens of proteins. (wikipedia.org)
  • They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. (nih.gov)
  • Aminoglycosides block protein synthesis by binding to bacterial 16S rRNA of the 30S ribosomal subunit. (cdc.gov)
  • In bacterial ribosomes, the small (30S) ribosomal subunit is composed of 16S rRNA and 21 distinct proteins. (unboundmedicine.com)
  • Ribosomal protein S15 is of particular interest because it binds primarily to 16S rRNA and is required for assembly of the small subunit and for intersubunit association, thus representing a key element in the assembly of a whole ribosome. (unboundmedicine.com)
  • Evolutionary analysis comparing mitoribosomes from different species with bacterial counterparts, illustrates that the exit tunnel evolves via deletions in the rRNA and extensions of mitoribosomal proteins. (nature.com)
  • They are messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). (dekooktips.com)
  • rRNA forms ribosomes, which are essential in protein synthesis. (dekooktips.com)
  • rRNA and tRNA are involved in protein synthesis. (dekooktips.com)
  • The other two forms of RNA, ribosomal RNA (rRNA) and transfer RNA (tRNA), are involved in the process of ordering the amino acids to make the protein. (dekooktips.com)
  • There are three types of RNA involved in the translation process: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). (dekooktips.com)
  • The three main types of RNA directly involved in protein synthesis are messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA). (dekooktips.com)
  • Messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA) are the three major types of RNA. (dekooktips.com)
  • In eukaryotes, the mature small subunit contains one rRNA and about 30 proteins, while the large subunit is made up of three rRNAs and approximately 50 proteins. (uni-muenchen.de)
Mammalian3
  • Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. (nih.gov)
  • The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. (thermofisher.com)
  • Initiation of mammalian protein synthesis. (reactome.org)
Mitochondrial ribosomal protein1
  • The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders. (nih.gov)
Genes2
  • Among these effects, growth-defective 60S mutants increased synthesis of proteins involved in proteasome-mediated degradation, whereas 40S mutants accumulated mature 60S subunits and increased translation of ribosome biogenesis genes. (broadinstitute.org)
  • As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (ucsc.edu)
Homologous1
  • Therefore, different names may refer to homologous proteins from different organisms, while identical names do not necessarily denote homologous proteins. (wikipedia.org)
Gene12
  • Further information can be found in the ribosomal protein gene database (RPG). (wikipedia.org)
  • This gene encodes a ribosomal protein that is a component of the 60S ribosome subunit. (nih.gov)
  • This gene encodes a 39S subunit protein. (nih.gov)
  • The RPSA gene provides instructions for making a protein called ribosomal protein SA, which is one of approximately 80 different ribosomal proteins. (medlineplus.gov)
  • RPSA gene mutations are thought to reduce the amount of functional ribosomal protein SA. (medlineplus.gov)
  • WD repeat domain 5 (WDR5) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. (thermofisher.com)
  • Small and Large Ribosomal Subunit Deficiencies Lead to Distinct Gene Expression Signatures that Reflect Cellular Growth Rate. (broadinstitute.org)
  • Because gene expression defects resulting from ribosome deficiency have not yet been experimentally defined, we systematically probed mRNA, translation, and protein signatures that were either unlinked from or linked to cellular growth rate in RP-deficient yeast cells. (broadinstitute.org)
  • however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. (ucsc.edu)
  • KJ892031 - Synthetic construct Homo sapiens clone ccsbBroadEn_01425 RPL22 gene, encodes complete protein. (ucsc.edu)
  • This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. (creativebiomart.net)
  • Unbuffered intronic mutations in gene variants can generate aberrant splice sites in mutant transcripts, resulting in mutant isoforms of proteins with modulated expression, stability, and function in diseased cells. (bvsalud.org)
MRNA8
  • Despite the proposed role for nerve growth factor (NGF) in this sprouting, we observed no change in NGF mRNA or protein at several postlesion time points. (jneurosci.org)
  • BC058887 - Homo sapiens ribosomal protein L22, mRNA (cDNA clone MGC:64893 IMAGE:6141451), complete cds. (ucsc.edu)
  • X59357 - Human mRNA for Epstein-Barr virus small RNAs (EBERs)associated protein (EAP). (ucsc.edu)
  • However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. (nature.com)
  • The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. (nature.com)
  • Binding of mRNA requires extended mitoribosomal proteins of the small subunit, and movement of tRNA is realized through additional proteins of the large subunit. (nature.com)
  • mRNA is the intermediary between the nucleus, where the DNA lives, and the cytoplasm, where proteins are made. (dekooktips.com)
  • Messenger RNA (mRNA) Carries information specifying amino acid sequences of proteins from DNA to ribosomes. (dekooktips.com)
Eukaryotic7
  • The 60S subunit is the large subunit of eukaryotic 80S ribosomes. (wikipedia.org)
  • In the figure "Crystal Structure of the Eukaryotic 60S Ribosomal Subunit from T. thermophila", the ribosomal RNA core is represented as a grey tube and expansion segments are shown in red. (wikipedia.org)
  • Crystal Structure of the Eukaryotic 60S Ribosomal Subunit from T. thermophila 60S subunit viewed from the subunit interface side, PDB identifiers 4A17, 4A19 60S subunit viewed from the solvent-exposed side, PDB identifiers 4A17, 4A19 The table "60S ribosomal proteins" shows the individual protein folds of the 60S subunit colored by conservation as above. (wikipedia.org)
  • It inactivates eukaryotic 60S ribosomal subunits. (joplink.net)
  • Protein synthesis in mitochondria supports bioenergetics of eukaryotic cells and is executed by dedicated mitoribosomes. (nature.com)
  • Eukaryotic (i.e. nucleated) cells can produce ribosomes in enormous numbers, although each consists of about 80 proteins and 4 ribosomal RNAs (rRNAs). (uni-muenchen.de)
  • Function of eukaryotic initiation factor 5 in the formation of an 80 S ribosomal polypeptide chain initiation complex. (reactome.org)
Ribosomes11
  • It is structurally and functionally related to the 50S subunit of 70S prokaryotic ribosomes. (wikipedia.org)
  • Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. (nih.gov)
  • These proteins come together to form structures called ribosomes. (medlineplus.gov)
  • Ribosomes process the cell's genetic instructions to create proteins. (medlineplus.gov)
  • Some ribosomal proteins are involved in the assembly or stability of ribosomes. (medlineplus.gov)
  • A shortage of the normal protein likely impairs the assembly of ribosomes, but the specific effects of the mutations are not known. (medlineplus.gov)
  • Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. (ucsc.edu)
  • RNA is then translated into proteins by structures called ribosomes. (dekooktips.com)
  • Ribosomes are the cell's protein factories. (uni-muenchen.de)
  • In order to carry out this vital task, the cell must ensure that it has enough of the complexes required for the synthesis of proteins - the ribosomes. (uni-muenchen.de)
  • Ribosomes are made up of two subunits, the small and the large, which are assembled separately and interact to form a single functional unit only when needed. (uni-muenchen.de)
Biogenesis5
  • Increased ribosomal biogenesis occurs during tissue hypertrophy, but whether ribosomal biogenesis is impaired during atrophy is not known. (nih.gov)
  • Decreased ribosomal content was accompanied by decreased expression of cMYC, a positive regulator of ribosomal biogenesis, as well as reduced expression and activity of β-catenin, a transcriptional activator of cMYC. (nih.gov)
  • Overexpressing β-catenin restored hyperammonemia-induced perturbations in signaling responses that regulate ribosomal biogenesis. (nih.gov)
  • An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation. (nature.com)
  • Moreover, some 200 other proteins known as biogenesis factors are necessary to ensure that the assembly process takes place without a hitch, and that all components of the functional ribosome find their proper places in its complex architecture. (uni-muenchen.de)
Mitochondria5
  • Ribosomal protein L10 in mitochondria serves as a regulator for ROS level in pancreatic cancer cells. (nih.gov)
  • Mitoribosomes are specialized protein synthesis machineries in mitochondria. (nature.com)
  • To provide a representative reference for studying protein synthesis in the mitochondria of fungi, and to reveal how the mitoribosome functions in coordination with its translation partners, we determined structures of the translating mitoribosome from the representative fungal model organism Neurospora crassa . (nature.com)
  • Therefore, to provide a reference for the process of protein synthesis in mitochondria, we set out to investigate the functional translation apparatus from the model organism N. crassa . (nature.com)
  • Studies on iron homeostasis in mitochondria: X-ray structures of yeast frataxin, a mitochondrial protein involved in iron storage and detoxification, also known as a factor in the neurodegenerative disease Friedrich's Ataxia. (lu.se)
Large 60S1
  • Hydrolysis of eIF2-GTP occurs after the Met-tRNAi has recognized the AUG. This reaction is catalyzed by eIF5 (or eIF5B) and is thought to cause dissociation of all other initiation factors and allow joining of the large 60S ribosomal subunit. (reactome.org)
Thermus1
  • We present a detailed analysis of the protein structures in the 30 S ribosomal subunit from Thermus thermophilus, and their interactions with 16 S RNA based on a crystal structure at 3.05 Å resolution. (caltech.edu)
Polypeptide2
  • With 20 different polypeptide chains, the 30 S subunit adds significantly to our data base of RNA structure and protein-RNA interactions. (caltech.edu)
  • Following 60S subunit joining, eIF5B hydrolyzes its GTP and is released from the 80S ribosome, which is now ready to start elongating the polypeptide chain. (reactome.org)
Binds3
  • The antibiotic binds preferentially to the 50S ribosomal subunit and affects the process of peptide chain initiation. (theodora.com)
  • It is a bacteriostatic antibiotic that has a mechanism of action that binds to the 23s subunit of the 50s ribosome that prevents bacterial protein synthesis. (dentistryiq.com)
  • The catalytic subunit of an anaerobic ribonucleotide reductase: What binds where? (lu.se)
Sequence4
  • Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. (nih.gov)
  • Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)
  • Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
  • Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)
Eukaryotes3
  • Proteins which have homologs in eukaryotes, archaea and bacteria are shown as blue ribbons. (wikipedia.org)
  • Proteins shared only between eukaryotes and archaea are shown as orange ribbons and proteins specific to eukaryotes are shown as red ribbons. (wikipedia.org)
  • The joining of ribosomal subunits in eukaryotes requires eIF5B. (reactome.org)
Interactions5
  • Analysis of the protein-RNA interactions in the context of ribosomal assembly shows that the primary binders are globular proteins that bind at RNA multihelix junctions, whereas proteins with long extensions assemble later. (caltech.edu)
  • Overexpression of GSK3β-resistant variants, genetic depletion of IκB kinase β (IKKβ) (activated during hyperammonemia), protein interactions, and in vitro kinase assays showed that IKKβ phosphorylated β-catenin directly. (nih.gov)
  • The latter is constrained by a conserved base triple and stacking interactions, and locked into place by magnesium ions and protein side chains, mainly through interactions with the unique three-dimensional geometry of the backbone. (unboundmedicine.com)
  • No yeast two-hybrid interactions found for this protein. (yeastrc.org)
  • Impaired spatial and temporal tethering of lamin proteins to the lamina or nucleoplasmic aggregation of lamins are the primary molecular events that can trigger nuclear proteotoxicity by modulating differential protein-protein interactions, sequestering quality control proteins, and initiating a cascade of abnormal post-translational modifications. (bvsalud.org)
Approximately 801
  • Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. (ucsc.edu)
Species2
  • Information about cross-species functional complementation between yeast and other species, curated by SGD and the Princeton Protein Orthology Database (P-POD). (yeastgenome.org)
  • For identification of Trichosporon species, the intergenic region of the ribosomal cistron is used (Sugita, 2002). (cdc.gov)
Bacterial protein synthesis3
  • Doxycycline inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit. (nih.gov)
  • Clindamycin inhibits bacterial protein synthesis at the level of the bacterial ribosome. (theodora.com)
  • Eravacycline disrupts bacterial protein synthesis by binding to the 30S ribosomal subunit thus preventing the incorporation of amino acid residues into elongating peptide chains. (centerwatch.com)
Tertiary structure2
  • In addition to globular domains, many of the proteins have long, extended regions, either in the termini or in internal loops, which make extensive contact to the RNA component and are involved in stabilizing RNA tertiary structure. (caltech.edu)
  • What type of interaction is NOT directly responsible for the formation of tertiary structure in proteins? (flashcardmachine.com)
Structures3
  • We have used cryo-electron microscopy to determine the structures of intermediate forms of the small ribosomal subunit isolated from human cells. (uni-muenchen.de)
  • Studied on ribosomal proteins and elongation factor structure and function: Worked on 3D structures of elongation factor G (EF-G), ribosomal recycling factor (RRF), ribosomal proteins L1, L22, S8, TL5 and some mutants and complexes of these. (lu.se)
  • The X-ray structures of trimers of the protein and their complexes with various metals, single-particle electron microscopic structure of iron-free and iron-loaded 24-subunit oligomers. (lu.se)
Folds1
  • Many ribosomal proteins share similar α+β sandwich folds, but we show that the topology of this domain varies considerably, as do the ways in which the proteins interact with RNA. (caltech.edu)
RNAs2
Decreased protein synthesis1
  • Our data show that decreased protein synthesis during hyperammonemia is mediated via a novel GSK3β-independent, IKKβ-dependent impairment of the β-catenin-cMYC axis. (nih.gov)
Abstract1
  • ABSTRACT In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. (who.int)
Bind3
  • The related protein in chicken can bind to c-Jun and can repress c-Jun-mediated transcriptional activation. (nih.gov)
  • The mature small subunit can then bind to the large subunit, in association with a messenger RNA that provides the blueprint for protein synthesis. (uni-muenchen.de)
  • Their mechanism of action is to bind to the 30s ribosomal subunit and prevent protein synthesis. (dentistryiq.com)
Homologs1
  • The table "60S ribosomal proteins" cross-references the human ribosomal protein names with yeast, bacterial, and archaeal homologs. (wikipedia.org)
Prokaryotic1
  • However, the 60S subunit is much larger than the prokaryotic 50S subunit and contains many additional protein segments, as well as ribosomal RNA expansion segments. (wikipedia.org)
Nucleus2
  • Production of both subunits begins at the site of synthesis of the rRNAs in the cell nucleus. (uni-muenchen.de)
  • After the initial steps in assembly, the immature subunits are transported from the nucleus into the cytoplasm. (uni-muenchen.de)
Bacteriostatic1
  • It is bacteriostatic with a mechanism of action of inhibiting protein synthesis by binding to the 50s ribosome. (dentistryiq.com)
Complex4
  • No complex found for this protein. (yeastrc.org)
  • Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. (yeastrc.org)
  • An adaptor protein subunit of MTORC2 COMPLEX. (bvsalud.org)
  • They also identified mtDNA deletions in a "hotspot" in complex I respiratory chain subunits that were associated with dysregulation of oxidative stress and DNA damage response pathways in cohorts with sporadic PD and PDD. (medscape.com)
Synthase1
  • Image analysis of the matched maps identified 7 proteins that were either over- or down-expressed: activated protein kinase c receptor (LACK), alpha tubulin (×2), prostaglandin f2-alpha synthase, protein disulfide isomerase, vesicular transport protein and a hypothetical protein. (who.int)
External identifiers for the protein1
  • List of external identifiers for the protein from various database sources. (yeastgenome.org)
Mechanism of action of protein synthe1
  • The mechanism of action of protein synthesis initiation factors from rabbit reticulocytes. (reactome.org)
Translation2
  • There are three binding sites for tRNA, the A-site, P-site and E-site (see article on protein translation for details). (wikipedia.org)
  • The 60S subunit joins - a reaction catalyzed by eIF5 or eIF5B - resulting in a translation-competent 80S ribosome. (reactome.org)
Structurally1
  • LMU researchers have now structurally characterized late stages in the assembly of the human small ribosomal subunit, yielding detailed insights into their maturation principles. (uni-muenchen.de)
Distinct1
  • These distinct signatures of protein synthesis suggest intriguing and currently mysterious differences in the cellular consequences of deficiency for small and large ribosomal subunits. (broadinstitute.org)
Proteomics1
  • The study shows the usefulness of proteomics in identifying proteins that may express differences between sensitive and resistant L. tropica isolates. (who.int)
Tetracyclines1
  • Tetracyclines are readily absorbed and are bound to plasma proteins in varying degree. (nih.gov)