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  • gene
  • To test this possibility, we conditionally deleted the gene encoding 40 S ribosomal protein S6 in the liver of adult mice. (sciencemag.org)
  • The effect of deleting the S6 gene was then examined in two experimental paradigms thought to require biosynthesis of nascent ribosomes: (i) nutrient-induced growth after a period of fasting, and (ii) mitogen-stimulated proliferation after partial hepatectomy ( 6 ). (sciencemag.org)
  • The mouse S6 gene was cloned ( 8 ), inserted into a loxP-targeting vector ( Fig. 1 A), and introduced into embryonic stem (ES) cells by homologous recombination ( 9 ). (sciencemag.org)
  • As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (nih.gov)
  • mRNA
  • A change in the ratio of ribosomes to mRNA can modify the pattern of proteins synthesized ( 2 ), ultimately leading to deleterious effects on the cell. (sciencemag.org)
  • Livers of IFN-α-treated fS6/CRE + mice contained no detectable S6 mRNA, but mRNA for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and other ribosomal proteins was present in similar amounts as in fS6/CRE − mice ( Fig. 1 D) ( 11 ). (sciencemag.org)
  • The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. (nih.gov)
  • ribosomes
  • Unexpectedly, livers from fasted animals deficient in S6 grew in response to nutrients even though biogenesis of 40 S ribosomes was abolished. (sciencemag.org)
  • A proliferating eukaryotic cell expends 80% of its energy on the biosynthesis of protein synthetic components, most notably ribosomes. (sciencemag.org)
  • They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. (nih.gov)
  • assay
  • CST's PathScan® Phospho-ALK (Tyr1604) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-ALK (Tyr1604) or phospho-NPM-ALK fusion protein. (cellsignal.com)
  • Inhibition
  • Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. (nih.gov)
  • antibodies
  • The magnitude of absorbance for this developed color is proportional to the quantity of phospho-ALK (Tyr1604) or phospho-NPM-ALK proteins.Antibodies in kit are custom formulations specific to kit. (cellsignal.com)
  • therapeutic target
  • Taken together, this project has the potential to provide initial evidence that correcting defective S6 regulation may serve as a therapeutic target and biomarker for a subgroup of patients with autism. (autismspeaks.org)
  • biochemical
  • Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. (nih.gov)
  • molecular
  • Recent research has provided evidence that in many different forms of autism spectrum disorders, the molecular mechanisms regulating the production of proteins are defective. (autismspeaks.org)
  • sequence
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • To investigate how protein folding is affected by sequence connectivity five topological variants of the ribosomal protein S6 were constructed through circular permutation. (openthesis.org)
  • histone
  • Background: F-box and leucine-rich repeat protein 10 (FBXL10), also known as lysine-specific demethylase 2B (KDM2B) and JmjC domain-containing histone demethylation protein 1B (JHDM1B), is a lysine-specific histone demethylase protein that demethylates Lys4 and Lys36 of histone H3 (1,2). (cellsignal.com)
  • Growth
  • The activity of this protein has been implicated in controlling cell growth and differentiation. (nih.gov)
  • tissues
  • By performing unbiased 2-dimensional electrophoresis of protein extracts from control rat heart tissues and EAM rat heart tissues, followed by nano-HPLC-ESI-QIT-MS, 67 proteins were identified from 71 spots that exhibited significantly altered expression levels. (biomedcentral.com)
  • Even though only two out of 29 down-regulated proteins in EAM rat heart tissues were also dysregulated in CVB-infected rat heart tissues, other proteins known to be involved with the generation of precursor metabolites and energy metabolism in mitochondria were also dysregulated in CVB-mediated myocarditis rat heart tissues, suggesting that impairment of mitochondrial functions may be a common underlying mechanism of the two murine myocarditis models. (biomedcentral.com)
  • Coordinates
  • The data were processed using the , using the coordinates of the protein portion of the phosphorylated S6K1KDstaurosporine complex (PDB: 3A62) as the initial search model. (cancer-basics.com)
  • known
  • Assignment of Homology to Genome Sequences using a Library of Hidden Markov Models that Represent all Proteins of Known Structure. (bris.ac.uk)
  • mice
  • Heterozygous mice were generated and crossed to obtain homozygous floxed S6 (fS6) offspring ( Fig. 1 B), which were phenotypically normal. (sciencemag.org)
  • mouse
  • Following extensive washing, an ALK Mouse mAb is added to detect the captured phospho-ALK or phospho-NPM-ALK fusion protein. (cellsignal.com)
  • Source
  • p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc. (uniprot.org)
  • different
  • However, this motif is recruited in different parts of the S6 structure depending on the permutation, either in the ?1 or ?2 half of the protein. (openthesis.org)