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  • particles
  • New evidence published in Cell by Vivian Budnik, PhD, Melissa J. Moore, PhD, and colleagues at the University of Massachusetts Medical School, reveals a novel budding mechanism, similar to the process used by some viruses, capable of exporting large ribonucleoprotein particles from the nucleus to the cytoplasm. (phys.org)
  • Working to understand how synapses develop and communicate with neighboring muscle cells, Budnik discovered a new method whereby these large granules, in the form of ribonucleoprotein (RNP) particles, were transported across the nuclear envelope. (phys.org)
  • Cas9
  • Here, we devise polymeric CRISPR/Cas9 system based on poly-ribonucleoprotein (RNP) nanoparticles consisting of polymeric sgRNA, siRNA, and Cas9 endonuclease in order to improve the delivery efficiency. (sigmaaldrich.com)
  • C ) The crRNA forms a ribonucleoprotein complex with Cas9, which recognizes invading DNA homologous to the crRNA sequence and mediates interference. (pnas.org)
  • Specifically, we showed that in the CRISPR3 system of Streptococcus thermophilus (a model and active type II CRISPR/Cas system), Cas9 associates with crRNA to form an effector complex that specifically cleaves matching target dsDNA ( Fig. P1 ), in sharp to the effector complexes for type I and type III systems, which are multisubunit ribonucleoprotein complexes. (pnas.org)
  • CRISPR
  • Here we report that single-step codelivery of CRISPR/Cpf1 ribonucleoproteins with single-stranded DNA repair templates results in precise and targeted DNA replacement with as much as ∼10% efficiency in C. reinhardtii . (pnas.org)
  • tissue
  • Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/250 dilution, followed by Goat anti rabbit IgG(HRP) secondary and counterstained with Hematoxylin. (abcam.com)
  • complex
  • Viral assembly is complex as the virus incorporates an eight-partite genome of RNA (in the form of viral ribonucleoproteins, vRNPs), and viral genome assembly − with its implications to public health − is not completely understood. (biologists.org)