• Ribonucleases can be divided into endoribonucleases and exoribonucleases, and comprise several sub-classes within the EC 2.7 (for the phosphorolytic enzymes) and 3.1 (for the hydrolytic enzymes) classes of enzymes. (wikipedia.org)
  • one method of isolating it is to boil a crude cellular extract until all enzymes other than RNase A are denatured. (wikipedia.org)
  • 1977 ). Further, a correlation was found between this RNase activity and the synthesis of unusual 2′-5′ oligoadenylates (2-5A) (Fig. 1 ) by a family of enzymes called oligoadenylate synthetases (OAS) [(Baglioni et al. (springer.com)
  • The ribonuclease (RNase) family is comprised of a ubiquitous group of enzymes that share the common ability to catalyze the cleavage of RNA. (kenyon.edu)
  • RNA degradation by RNase serves as a defense mechanism against RNA viruses, provides the machinery for RNAi through the activity of the Dicer and Drosha enzymes, and has been recently targeted as a promising chemotherapeutic agent. (kenyon.edu)
  • For example, add 0.5 μl RNase to the nucleic acids from 10 6 cells and incubate at +15 to + 25 °C or +37 °C. For nucleic acids from 10 7 cells, add 1.5 μl RNase and incubate 30 min at + 37 °C. For nucleic acids from 10 8 cells, add 16 μl RNase and incubate 30 min at + 37 °C. (sigmaaldrich.com)
  • Thermostable RNase H is an endoribonuclease that is functional at high temperatures and selectively hydrolyzes the phosphodiester bonds of an RNA strand in an RNA:DNA hybrid molecule, while leaving the DNA strand intact. (neb.com)
  • the dissociation constant for the RI-RNase A complex is ~20 fM under physiological conditions. (wikipedia.org)
  • the dissociation constant of the RI-RNase A complex is in the femtomolar (fM) range under physiological conditions while that for the RI-angiogenin complex is less than 1 fM. (wikipedia.org)
  • RNase L activates the NLRP3 inflammasome during viral infections. (springer.com)
  • Cheney hypothesizes that CFS moves through three distinct stages that begin when an intracellular organism activates the RNase L pathway. (thefreedictionary.com)
  • An E. coli strain carrying a codon optimized plasmid encoding RNase H from the extreme thermophile Thermus thermophilus . (neb.com)
  • One of the best-described IFN-dependent antiviral responses is the OAS/RNase L pathway. (springer.com)
  • Han Y, Whitney G, Donovan J, Korennykh A. Innate immune messenger 2-5A tethers human RNase L into active high-order complexes. (springer.com)
  • Han Y, Donovan J, Rath S, Whitney G, Chitrakar A, Korennykh A. Structure of human RNase L reveals the basis for regulated RNA decay in the IFN response. (springer.com)
  • NOTE: The RNAse H Reaction Buffer contains MgCl 2 . (neb.com)
  • This thermostable nuclease exhibits the same enzymatic properties as E. coli RNase H, but is active at much higher temperatures. (neb.com)
  • 6 The concentration for use is 250-1000 units/mL. 7 Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause release of active ribonuclease from the complex. (sigmaaldrich.com)
  • Because the exact concentration of RNA in biological samples is not known exactly, and one typically wants to degrade the RNA quantitatively, a excess of RNase is recommended. (sigmaaldrich.com)
  • Parniak, Professor of Molecular Genetics & Biochemistry at the University of Pittsburgh and Principal Investigator of this multi-team research effort, has nearly 20 years experience in HIV research and is regarded as a thought leader in the discovery of drugs targeting HIV RNase H. (thefreedictionary.com)
  • Both biochemical studies and crystallographic structures of RI-RNase A complexes suggest that the interaction is governed largely by electrostatic interactions, but also involves substantial buried surface area. (wikipedia.org)
  • Gusho E, Baskar D, Banerjee S. New advances in our understanding of the "unique" RNase L in host pathogen interaction and immune signaling. (springer.com)
  • Hydrogen bonding of Lys41 with hRI is the strongest and most conserved interaction between hRI and other RNase variants. (kenyon.edu)
  • RNase A was also the first model protein for the development of several chemical structural methods, such as limited proteolysis of disordered segments, chemical modification of exposed side chains, and antigenic recognition. (bionity.com)
  • Arg91 has the greatest influence on the stability of the hRI:RNase 1 complex, 2.7 kcal/mol. (kenyon.edu)
  • RNase A was the model protein used to work out many spectroscopic methods for assaying protein structure, including absorbance, circular dichroism/optical rotary dispersion, Raman, EPR and NMR spectroscopy. (bionity.com)
  • RNase A was the third protein to have its structure solved, in 1967. (bionity.com)
  • Ribonuclease was selected since a great deal is known about its structure from the work of Carlisle et al. (springer.com)
  • RNase B is a high mannose glycoprotein that can be used as a positive control for endoglycosidases that cleave N-linked carbohydrates. (neb.com)