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  • inhibitor
  • RiboSafe RNase Inhibitor is a recombinant protein which completely inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C. (bioline.com)
  • RiboSafe RNase Inhibitor is a recombinant protein which completely inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C. Furthermore, RiboSafe shows no inhibition of polymerase or reverse transcriptase activity and so can be used for cDNA synthesis and in one-step RT-PCR reactions. (bioline.com)
  • RiboSafe RNase Inhibitor is tested for activity, SDS-PAGE purity and the absence of endonucleases, nickases and exonucleases and functions robustly at a variety of temperatures and pH and in the presence or absence of DTT, allowing flexibility in designing experiments in highly-sensitive techniques such as single-cell RT-PCR, in vitro RNA synthesis and in vitro translation. (bioline.com)
  • Either RNase A and RNase inhibitor were incubated directly with yeast total RNA (blue column) or RNase A and RNase inhibitor were mixed and pre-incubated before addition to yeast total RNA (grey column). (bioline.com)
  • In both cases using RiboSafe RNase Inhibitor blocks more of the RNase A activity than RNase inhibitors from Supplier S. (bioline.com)
  • HeLa cell total RNA was incubated at 37°C for 30 min with RiboSafe RNase Inhibitor in the presence of between 2 ng - 125 pg of RNase A (Lanes 1-4), no RiboSafe RNase Inhibitor and 125 pg of RNase A (Lane 5) and no RiboSafe RNase Inhibitor, no RNase A (Lane 6). (bioline.com)
  • Even at high concentrations of RNase A, RiboSafe RNase Inhibitor showed no visible levels of degradation. (bioline.com)
  • A two-fold serial dilution of total HeLa cell total RNA (1 µg - 0.075 µg) was reverse transcribed in the presence (Lanes 1-5) and in the absence (Lanes 7-11) of RiboSafe RNase Inhibitor, followed by the amplification of a 1 kb fragment of the Angiotensin receptor II gene using MyFi Mix. (bioline.com)
  • There was no detectable difference in PCR product in the presence or absence of RiboSafe RNase Inhibitor. (bioline.com)
  • 2 µg aliquots of mouse total RNA was incubated at 37 o C with RiboSafe RNase Inhibitor and no RNase A (lane 1), no RiboSafe RNase Inhibitor and RNase A (lane 2), or with RiboSafe RNase Inhibitor and RNase A at 37 o C, 42 o C, 45 o C, 50 o C and 55 o C (lanes 3-7 respectively). (bioline.com)
  • Regardless of the temperature, there is no visible levels of degradation when incubated with RiboSafe RNase Inhibitor. (bioline.com)
  • A highly effective, broad-spectrum eukaryotic RNase inhibitor. (bioline.com)
  • Would you like to share your experience of the RiboSafe RNase Inhibitor as part of our new customer review program? (bioline.com)
  • enzymes
  • 1977 ). Further, a correlation was found between this RNase activity and the synthesis of unusual 2′-5′ oligoadenylates (2-5A) (Fig. 1 ) by a family of enzymes called oligoadenylate synthetases (OAS) [(Baglioni et al. (springer.com)
  • In eukaryotes RNase III or RNaseIII like enzymes such as Dicer are involved in RNAi (RNA interference) and miRNA (micro-RNA) gene silencing [ PMID: 14983173 , PMID: 15066275 , PMID: 11809414 ]. (ebi.ac.uk)
  • RNase III enzymes and the initiation of gene silencing. (ebi.ac.uk)
  • Characterization of Rny1, the Saccharomyces cerevisiae member of the T2 RNase family of RNases: unexpected functions for ancient enzymes? (ebi.ac.uk)
  • RNase HI belongs to the family of ribonuclease H enzymes that recognise RNA:DNA hybrids and degrade the RNA component. (ebi.ac.uk)
  • The ribonuclease (RNase) family is comprised of a ubiquitous group of enzymes that share the common ability to catalyze the cleavage of RNA. (kenyon.edu)
  • RNA degradation by RNase serves as a defense mechanism against RNA viruses, provides the machinery for RNAi through the activity of the Dicer and Drosha enzymes, and has been recently targeted as a promising chemotherapeutic agent. (kenyon.edu)
  • gene
  • RNase L attenuates mitogen-stimulated gene expression via transcriptional and post-transcriptional mechanisms to limit the proliferative response. (springer.com)
  • The S3 gene was mutagenized by replacing the codon for His-93, which has been implicated in ribonuclease activity, with a codon for asparagine, and the mutant S3 gene was introduced into P. inflata plants of S1S2 genotype. (mendeley.com)
  • He said that now that they have the human RNase L structure, we can begin to understand the effects of carcinogenic mutations in the RNase L gene. (thefreedictionary.com)
  • mRNA stability
  • Le Roy F, Silhol M, Salehzada T, Bisbal C. Regulation of mitochondrial mRNA stability by RNase L is translation-dependent and controls IFN alpha-induced apoptosis. (springer.com)
  • Prokaryotic RNAse III is important in post-transcriptional control of mRNA stability and translational efficiency. (ebi.ac.uk)
  • amino
  • The amino acid sequence of ribonuclease N1, a guanine-specific ribonuclease from the fungus Neurospora crassa. (ebi.ac.uk)
  • RNase 1 is a 156 amino acid protein that resembles the shape of a kidney. (kenyon.edu)
  • 1. On the oxidation of a linear partially protected octapeptide corresponding to the sequence of amino acids of the ribonuclease chain in the 65-72 segment, the corresponding disulfide is formed in high yield, which apparently shows the definite predisposition of this fragment to the formation of a disulfide bond between the terminal cysteines. (springer.com)
  • ribosomal
  • NMR structure of the N-terminal domain of Saccharomyces cerevisiae RNase HI reveals a fold with a strong resemblance to the N-terminal domain of ribosomal protein L9. (ebi.ac.uk)
  • activity
  • Back in the 1970s, the groups of I. Kerr and P. Lengyel discovered a cellular endoribonuclease (RNase) activity that was increased by IFN and depended on the presence of double-stranded RNA (dsRNA) (Brown et al. (springer.com)
  • Dimeric structure of pseudokinase RNase L bound to 2-5A reveals a basis for interferon-induced antiviral activity. (springer.com)
  • Here, the ribonuclease activity of S3 protein of P. inflata was abolished, and the effect on the pistil's ability to reject S3 pollen was examined. (mendeley.com)
  • The mutant S3 protein produced in these two transgenic plants did not exhibit any detectable ribonuclease activity. (mendeley.com)
  • However, RNase activity can be detrimental when unregulated. (kenyon.edu)
  • Intracellular RNAs that are not targeted for degradation are protected from RNase activity by a number of different mechanisms. (kenyon.edu)
  • This entry represents the regulator of ribonuclease E activity A (RraA). (ebi.ac.uk)
  • residues
  • A total number of 23 residues of RNase 1 make contact with hRI. (kenyon.edu)
  • Additionally, regions of high shape complementarity between residues of hRI and RNase 1 are ultimately responsible for the protein-protein interaction affinity in the femtomolar range. (kenyon.edu)
  • Amongst all of the residues responsibe for intermolecular hydrogen bonding between regions of high shape complementarity in the hRI:RNase 1 complex, three residues in particular provide the highest energetic contribution to complex stabilization. (kenyon.edu)
  • interaction
  • Both biochemical studies and crystallographic structures of RI-RNase A complexes suggest that the interaction is governed largely by electrostatic interactions, but also involves substantial buried surface area . (wikipedia.org)
  • Gusho E, Baskar D, Banerjee S. New advances in our understanding of the "unique" RNase L in host pathogen interaction and immune signaling. (springer.com)
  • Hydrogen bonding of Lys41 with hRI is the strongest and most conserved interaction between hRI and other RNase variants. (kenyon.edu)
  • Structure
  • Crystal structure of a ribonuclease from the seeds of bitter gourd (Momordica charantia) at 1.75 A resolution. (ebi.ac.uk)
  • Ribonuclease was selected since a great deal is known about its structure from the work of Carlisle et al. (springer.com)
  • Subsite binding in an RNase: structure of a barnase-tetranucleotide complex at 1.76-A resolution. (ebi.ac.uk)
  • complex
  • the dissociation constant of the RI- RNase A complex is in the femtomolar (fM) range under physiological conditions while that for the RI- angiogenin complex is less than 1 fM. (wikipedia.org)
  • 6 The concentration for use is 250-1000 units/mL. 7 Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause release of active ribonuclease from the complex. (sigmaaldrich.com)
  • These regions allow an extensive number of hydrogen bonds to be made between hRI and RNase 1 that serve to stabilize the complex and increase the efficiency of RNase evasion. (kenyon.edu)
  • Arg91 has the greatest influence on the stability of the hRI:RNase 1 complex, 2.7 kcal/mol. (kenyon.edu)
  • A complex of RNase A with a transition-state analog, uridine vanadate, was also studied using a combination of neutron and x-ray diffraction techniques (14). (thefreedictionary.com)