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rhoB GTP-Binding ProteinGTP-Binding ProteinsGuanosine TriphosphateGTP PhosphohydrolasesGuanosine DiphosphateMolecular Sequence DataMonomeric GTP-Binding Proteinsrab GTP-Binding ProteinsGuanosine 5'-O-(3-Thiotriphosphate)Amino Acid SequenceADP-Ribosylation FactorsPertussis Toxinrho GTP-Binding ProteinsVirulence Factors, BordetellaAdenosine Diphosphate RiboseProtein BindingADP Ribose TransferasesBotulinum ToxinsBase SequenceThionucleotidesrhoA GTP-Binding ProteinSequence Homology, Amino AcidGuanine Nucleotide Exchange FactorsGTPase-Activating Proteinsras ProteinsCloning, MolecularSignal TransductionMutationPoly(A)-Binding ProteinsKineticsCarrier ProteinsCell MembraneCattleSaccharomyces cerevisiaeBinding SitesGTP CyclohydrolaseTacrolimus Binding ProteinsGuanine NucleotidesHydrolysisPoly(A)-Binding Protein IProtein Structure, TertiaryDNA-Binding ProteinsGuanylyl ImidodiphosphateInsulin-Like Growth Factor Binding ProteinsRecombinant ProteinsCell LineProtein PrenylationRNA-Binding ProteinsRNA, MessengerEscherichia coliModels, MolecularFarnesyltranstransferaseFatty Acid-Binding ProteinsNuclear ProteinsRecombinant Fusion ProteinsGuanosine MonophosphatePeptide Elongation Factor TuTransducinTransglutaminasesTransfectionProto-Oncogene Proteins p21(ras)Membrane ProteinsPoly(A)-Binding Protein IIBacterial ProteinsADP-Ribosylation Factor 1Peptide Elongation Factor GGTP Phosphohydrolase-Linked Elongation FactorsSequence AlignmentMutagenesis, Site-DirectedDynaminsElectrophoresis, Polyacrylamide GelBlotting, WesternTranscription FactorsDNA, ComplementaryInsulin-Like Growth Factor Binding Protein 3HeLa CellsGene Expression RegulationMolecular WeightProtein ConformationPeriplasmic Binding ProteinsCalcium-Binding ProteinsCells, CulturedAdenylosuccinate SynthasePhosphorylationTwo-Hybrid System Techniques3T3 CellsPeptide Elongation FactorsDNADNA PrimersTacrolimus Binding Protein 1ATubulinEnzyme ActivationPromoter Regions, GeneticLatent TGF-beta Binding ProteinsRibosomesrab5 GTP-Binding ProteinsAlkyl and Aryl Transferasesrab2 GTP-Binding ProteinCalciumProtein Biosynthesis
Small GTP-bindingRhoCGTPaseActive GTP-bound formKinasesRhoA and RhoBSignal TransductionDissociation inhibitorRegulateGTPasesTumorGuanine nucleoSuperfamilyGeneAntibodiesEffectorInhibitsKinaseHigh affinityHumansReceptorsInhibitoryAntibodyCharacterizationAffinitySequenceEnzymeReceptor1996SerineGenesLocalizationOrthologousRegulationGeranylgeranylActin cytoskeletonShown to bindMotifsUpregulationRhotekinFormCellular functions
Small GTP-binding2
RhoC5
  • RhoA functions in the regulation of actomyosin contractility, RhoB has been shown to regulate cytokine trafficking and cell survival, while RhoC appears to be important in cell locomotion. (wjon.org)
  • The Rho proteins RhoA, RhoB, and RhoC are highly homologous and contain the consensus amino acid sequences necessary for GDP/GTP-binding and GTPase activity. (phosphosolutions.com)
  • This peptide sequence is highly conserved in rat, mouse, and chicken RhoA, but is not found in RhoB or RhoC proteins. (phosphosolutions.com)
  • Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. (bionotatki.com)
  • The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit. (bionotatki.com)
GTPase12
  • The conversion from the GDP-bound to GTP-bound form and the reverse conversion are induced by GDP/GTP exchange and GTPase reactions, respectively. (nii.ac.jp)
  • We have purified and characterized several GDP/GTP exchange proteins (GDP dissociation stimulator (GDS) and GDP dissociation inhibitor (GDI)) and GTPase activating Proteins (GAP) for small G proteins. (nii.ac.jp)
  • Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. (cusabio.com)
  • Term] id: PR:000013977 name: Rho-related GTP-binding protein RhoB def: "A Rho-related small GTPase that is a translation product of the human RHOB gene or a 1:1 ortholog thereof. (proconsortium.org)
  • Rho-related small GTPase [Term] id: PR:Q23862 name: Rho-related protein racE (Dictyostelium discoideum) def: "A Rho-related GTP-binding protein RhoB that is encoded in the genome of Dictyostelium discoideum. (proconsortium.org)
  • Interconversion between these forms is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). (phosphosolutions.com)
  • A structural feature that distinguishes the Rho proteins from other small GTPases is the so-called Rho insert domain located between a β strand and an α helix within the small GTPase domain [ 1 - 3 ]. (biomedcentral.com)
  • Typically Rho proteins are 190-250 residues long and consist only of the GTPase domain and short terminal C-terminal extensions. (biomedcentral.com)
  • Within their GTPase domains, they share approximately 30% amino acid identity with the Ras proteins and 40-95% identity within the family. (biomedcentral.com)
  • Rho GTPase effectors are a large group of proteins and include actin nucleation promoting molecules, adaptors, as well as kinases. (biomedcentral.com)
  • Growth inhibitory proteins in the central nervous system (CNS) block axon growth and regeneration by signaling to Rho, an intracellular GTPase. (silverchair.com)
  • Although a report has shown that RhoB, a small GTPase involved in cytoskeletal actin rearrangement, lies downstream of Slug ( 11 ), another member of the Snail super-family, there is no evidence to support a link between Snail and Rho GTPases. (spandidos-publications.com)
Active GTP-bound form3
  • The Rho proteins cycle within the cell between the active GTP-bound form and the inactive GDP-bound form, the cycle being regulated by a number of activators and inhibitors. (wjon.org)
  • Rho cycles between the active GTP-bound form and an inactive GDP-bound form. (phosphosolutions.com)
  • Rho GTPases work as sensitive molecular switches existing either in an inactive, GDP-bound form or an active GTP-bound form. (biomedcentral.com)
Kinases3
  • This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). (phosphosolutions.com)
  • Similar rho-binding domains also occur in a number of other protein kinases and in the rho-binding proteins rhophilin and rhotekin. (embl-heidelberg.de)
  • 6. Somlyo AP, Somlyo AV. Ca2+ sensitivity of smooth muscle and nonmuscle myosin II: modulated by G proteins, kinases, and myosin phosphatase. (almazovcentre.ru)
RhoA and RhoB1
Signal Transduction2
  • This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. (cancerindex.org)
  • A RHO GTP-BINDING PROTEIN involved in regulating signal transduction pathways that control assembly of focal adhesions and actin stress fibers. (nih.gov)
Dissociation inhibitor1
  • ARHGDIG is a GDP dissociation inhibitor (GDI) that binds to RHOB and inhibits its activation (Zalcman et al. (reactome.org)
Regulate4
GTPases6
  • Human RAS superfamily proteins and related GTPases. (xenbase.org)
  • Rho GTPases are members of the Ras superfamily of GTPases with at least 20 Rho proteins having been characterized. (wjon.org)
  • Rho (A, B, & C) proteins are members of the Ras superfamily of GTPases. (phosphosolutions.com)
  • with us one can be cytosol proteins, " badenova( 2009) is four primary photoreceptors, the most non-crossover-generating of which chloroplasts release, phosphorylated, and ready ataxia bacterial to a cascade of challenging glycoprotein GTPases. (evakoch.com)
  • The exchange of GDP to GTP and thus the activation of Rho GTPases is catalyzed by guanine nucleotide exchange factors (GEFs), which act downstream of numerous growth factor receptors, integrins, cytokine receptors, and cadherins. (biomedcentral.com)
  • In turn, GTP-bound active GTPases can interact with a plethora of different effectors which mediate the different cellular functions of this family of proteins. (biomedcentral.com)
Tumor1
  • In addition to its role in controlling cell cycle progression, the tumor suppressor protein p53 can also affect other cellular functions such as cell migration. (rupress.org)
Guanine nucleo1
  • Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center. (cusabio.com)
Superfamily2
Gene7
  • Ras homolog gene family, member B, also known as RHOB, is a protein which in humans is encoded by the RHOB gene. (wikipedia.org)
  • Entrez Gene: RHOB ras homolog gene family, member B". Chardin P, Madaule P, Tavitian A (March 1988). (wikipedia.org)
  • The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. (cancerindex.org)
  • What does this gene/protein do? (cancerindex.org)
  • Gene structures and chromosomal locations of human protein prenyltransferase subunit genes. (biomedcentral.com)
  • The RPA gene is the structural excretion loss-of-function, However Binding it from the corresponding growth( De Laat et al. (evakoch.com)
  • with Pubmed ID, gene symbol/accession number and viral interacting protein. (polygenicpathways.co.uk)
Antibodies2
  • In this research project, we have investigated the C-terminal structures of small G proteins, purified the regulatory proteins for small G proteins, and made monoclonal antibodies against small G proteins and their regulatory proteins. (nii.ac.jp)
  • Among these regulatory proteins, we have cloned the cDNAs of smg p21 GDS, smg p25A GDI, and rho GDI, and made monoclonal antibodies against them. (nii.ac.jp)
Effector2
  • The fact that many Rho family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors RhoA protein activation. (cytoskeleton.com)
  • The assay uses the Rho binding domain (RBD) of the Rho effector protein, Rhotekin. (cytoskeleton.com)
Inhibits1
Kinase1
  • The morphological transition requires the RhoA-ROCK (Rho-associated coil-containing protein kinase) pathway and is prevented by RhoE. (rupress.org)
High affinity2
  • All members contain the sequence motifs characteristic of all GTP-binding proteins, bind to GDP and GTP with high affinity. (biomedcentral.com)
  • The fact that the RBD region of Rhotekin has a high affinity for GTP-RhoA and that Rhotekin binding results in a significantly reduced intrinsic and catalytic rate of GTP hydrolysis make it an ideal tool for affinity purification of GTP-RhoA from cell lysates. (cytoskeleton.com)
Humans1
  • There are three different protein prenyltransferases in humans: farnesyltransferase (FT) and geranylgeranyltransferase 1 (GGT1) share the same motif (the CaaX box) around the cysteine in their substrates, and are thus called CaaX prenyltransferases, whereas geranylgeranyltransferase 2 (GGT2, also called Rab geranylgeranyltransferase) recognizes a different motif and is thus called a non-CaaX prenyltransferase [ 1 ]. (biomedcentral.com)
Receptors2
  • The 3' replication has freely activated by RNase Z surface, a mature binding in receptors( reviewed in Maraia and Lamichhane 2011). (evakoch.com)
  • Recent data indicate that these three inhibitory proteins bind to the same neuronal receptor, the Nogo-66 receptor (NgR), but there are additional inhibitory proteins in the CNS that act through different receptors. (silverchair.com)
Inhibitory2
  • Publications] Sasaki,T.: 'A mammalian inhibitory GDP/GTP exchange protein(GDI)for smg p25A is active on the yeast SEC4 protein. (nii.ac.jp)
  • The use of C3 transferase to inactivate Rho in primary neurons plated on various types of inhibitory proteins and dominant-negative Rho-expressing PC-12 cells provides direct evidence that the inactivation of Rho results in neurite outgrowth on inhibitory substrates. (silverchair.com)
Antibody1
  • The antibody detects a RhoA GST fusion protein when phosphorylated with PKA, but does not detect the unphosphorylated recombinant protein. (phosphosolutions.com)
Characterization2
  • Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP" (PDF). (wikipedia.org)
  • Publications] Kaibuchi,K.: 'Molecular cloning and characterization of the stimulatory GDP/GTP exchange protein(GDS)for smg p21s,ras p21ーlike small GTPーbinding proteins. (nii.ac.jp)
Affinity1
  • The Rhotekin-RBD protein supplied in this kit contains Rhotekin residues 7-89 and is in the form of a GST fusion protein, which allows one to "pull-down" the Rhotekin-RBD/Rho-GTP complex with brightly colored glutathione affinity beads. (cytoskeleton.com)
Sequence3
  • Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. (cusabio.com)
  • Automatic comparisons of data from expressed sequence tags (ESTs) with genes (for example using the program Acembly, for which the results are available from the NCBI AceView server [ 2 ]) shows that all the human protein prenyltransferase genes have multiple alternative splice variants. (biomedcentral.com)
  • Rho-related GTP-binding protein RhoB [Term] id: PR:000040021 name: Rho-related GTP-binding protein RhoB isoform 1 def: "A Rho-related GTP-binding protein RhoB that is a translation product of some mRNA whose exon structure and start site selection renders it capable of giving rise to a protein with the amino acid sequence represented by UniProtKB:P62745-1 or a 1:1 ortholog thereof. (proconsortium.org)
Enzyme2
  • This download is the types and cells led from a human assembly target content soccer methylated alongside the set of two interaction localizing enzyme proteins in New Zealand. (evakoch.com)
  • The extracellular signaling protein Sonic hedgehog (Shh) requires dual lipidation for functional signaling, whereby N-terminal palmitoylation is performed by the enzyme Hedgehog acyltransferase (Hhat). (imperial.ac.uk)
Receptor3
  • ELAC1 has been in the use and may as define as an RNase Z. In pyrophosphates subfamilies are transcribed from coupling tubules in the function by a two receptor chromatin that appears ultraviolet from protein threatening( reviewed in Popow et al. (evakoch.com)
  • Starch binding domain-containing protein 1 (Stbd1) is a carbohydrate-binding protein that has been proposed to be a selective autophagy receptor for glycogen. (imperial.ac.uk)
  • adhesion G protein-coupled receptor E5. (gsea-msigdb.org)
19961
  • 1996). Binding of other GDIs to RHOB has not been tested. (reactome.org)
Serine1
Genes5
  • The genes encoding CaaX protein prenyltransferases are considerably longer than those encoding non-CaaX subunits, as a result of longer introns. (biomedcentral.com)
  • The genomic organization of the human genes that encode protein prenyltransferases is shown in Figure 1 . (biomedcentral.com)
  • (b) genes encoding non-CaaX protein prenyltransferases are much shorter. (biomedcentral.com)
  • The chromosomal locations and number of exons from protein prenyltransferase genes in the major eukaryotic model organisms are shown in Table 2 . (biomedcentral.com)
  • To date, 1527 human miRNAs have been identified (Sanger miRBase 18 http://www.miRbase.org/index.shtml), forming less than 1% of all human genes, potentially regulating more than 10% of all protein coding genes [ 1 ]. (intechopen.com)
Localization1
  • In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. (cusabio.com)
Orthologous2
  • This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. (embl-heidelberg.de)
  • Percentage points are related to the number of proteins with Hr1 domain which could be assigned to a KEGG orthologous group, and not all proteins containing Hr1 domain. (embl-heidelberg.de)
Regulation1
  • Evidence is accumulating that small G proteins are involved in the regulation of cell growth and differentiation. (nii.ac.jp)
Geranylgeranyl1
  • Protein prenylation is the posttranslational attachment of either a farnesyl group or a geranylgeranyl group via a thioether linkage (-C-S-C-) to a cysteine at or near the carboxyl terminus of the protein. (biomedcentral.com)
Actin cytoskeleton1
Shown to bind2
  • The first two of these repeats were later shown to bind the small G protein rho [ ( PUBMED:8647255 ) ( PUBMED:9446575 ) ] known to activate PKN in its GTP-bound form. (embl-heidelberg.de)
  • The RBD motif has been shown to bind specifically to the GTP-bound form of RhoA. (cytoskeleton.com)
Motifs1
  • Most of small G proteins have the unique consensus C-terminal motifs containing at least one cysteine residue. (nii.ac.jp)
Upregulation1
  • LCN2 protein induced upregulation of glial fibrillary acidic protein (GFAP), cell migration, and morphological changes similar to characteristic phenotypic changes termed reactive astrocytosis. (jneurosci.org)
Rhotekin1
  • GST-tagged Rhotekin-RBD protein on colored agarose beads (Cat. (cytoskeleton.com)
Form1
  • Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. (bionotatki.com)
Cellular functions1
  • We discuss how recent chemical proteomic approaches have delivered the first global overviews of protein lipidation in these organisms, contributing to our understanding of the role of this PTM in critical metabolic and cellular functions. (imperial.ac.uk)