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  • RHOA
  • Our results indicate that beta1 integrins regulate the polarity and motility of epithelial cells by the induction of intracellular molecular events involving a downregulation of alpha-catenin function and the activation of the Rho-like G proteins Rac1 and RhoA. (nih.gov)
  • RhoA (sometimes referred to as just 'Rho') is responsible for the formation of stress fibers, and its activity in stress fiber formation was first discovered by Ridley and Hall in 1992. (wikipedia.org)
  • cytoskeleton
  • Expression of beta1 integrins in GE11 cells resulted in a decrease in cadherin and alpha-catenin protein levels accompanied by their redistribution from the cytoskeleton-associated fraction to the detergent-soluble fraction. (nih.gov)
  • Molecules
  • Poly-(ADP-ribose) polymerases (PARPs) are found mostly in eukaryotes and catalyze the transfer of multiple ADP-ribose molecules to target proteins. (wikipedia.org)
  • regulates
  • The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). (cancerindex.org)
  • epitope-tagged
  • To examine the function of ECT2 as a Rho GEF, we expressed ECT2 as a FLAG epitope-tagged protein in COS cells immunoprecipitated with anti-FLAG mAb, and used immunoprecipitated protein for exchange assays. (nih.gov)
  • cells
  • Conversely, uptake of bacteria by CHO cells was promoted upon microinjection of an activated rho variant, Val14RhoA. (rupress.org)
  • The encoded protein is expressed in hematopoietic cells, where it functions as a negative regulator of cell growth and survival. (nih.gov)
  • ECT2 protein was detected with anti-ECT2 antibody in the cells as they progress through the cell cycle upon release from the drug arrest (upper left panel). (nih.gov)
  • Lysates of cells arrested at G1 phase by aphidicolin or at M phase by nocodazole were incubated with a protein phosphatase VHR, separated by SDS-PAGE, and analyzed for ECT2 (lower left panel). (nih.gov)
  • Mechanistically, Notch intracellular domain (NICD) protein abundance in H358 cells was regulated by Rnd3-mediated NICD proteasome degradation. (nih.gov)
  • RhoGDI3 is predominantly expressed in brain, lung, kidney, testis and pancreas, and is targeted to specific parts of the cell such as the Golgi where it may play a role in transport or proteins in cells. (wikipedia.org)
  • They are found in eukaryotic cells, as well as some bacteria, and are formed by the polymerization of a dimer of two globular proteins, alpha and beta tubulin. (wikipedia.org)
  • They are composed of actin (microfilaments) and non-muscle myosin II (NMMII), and also contain various crosslinking proteins, such as α-actinin, to form a highly regulated actomyosin structure within non-muscle cells. (wikipedia.org)
  • Adherens junctions are a type of cell-cell adhesion structure that is present in both motile and non-motile cells, which adhere cells together via the homophilic binding of cadherins and nexins. (wikipedia.org)