• Agrobacterium tumefaciens infects the plant through its Ti plasmid. (wikipedia.org)
  • The bacterium infects the plant through the rhizosphere, an area of soil along the plant root surface which contains microorganisms, making up the "external metabolome" (Bais et al. (richmond.edu)
  • Agrobacterium tumefaciens is an Alphaproteobacterium of the family Rhizobiaceae, which includes the nitrogen-fixing legume symbionts. (wikipedia.org)
  • Since the Ti plasmid is essential to cause disease, prepenetration events in the rhizosphere occur to promote bacterial conjugation - exchange of plasmids amongst bacteria. (wikipedia.org)
  • The accessory plasmid pAspH13-3a (0.6Mb) is only distantly related to the plasmid pAtC58 of A. tumefaciens C58 and shows a mosaic structure. (uni-bielefeld.de)
  • These data delineate the relationship between an individual Agrobacterium strain infiltration OD600, plant cell perimeter, and leaf age, as well as plant cell coinfection rates. (bvsalud.org)
  • Based on phylogenetic analyses, the isolate could be assigned to the genus Agrobacterium biovar I and represents a genomic species G1 strain within this biovariety. (uni-bielefeld.de)
  • Many strains of A. tumefaciens do not contain a pTi. (wikipedia.org)
  • Rhizosphere Competence - The most successful of the strains of biocontrol microorganisms exhibit rhizosphere competence, the ability to colonize and grow in association with plant roots. (umass.edu)
  • In the presence of opines, A. tumefaciens produces a diffusible conjugation signal called 30C8HSL or the Agrobacterium autoinducer[citation needed]. (wikipedia.org)
  • Currently, there are several reports on standardisation of protocol for the Agrobacterium-mediated transformation. (wikipedia.org)
  • Despite the value of Agrobacterium-mediated transient transformation of plants, MOI has not been quantified. (bvsalud.org)
  • Biological DNA transfer into plant cells mediated by Agrobacterium represents one of the most powerful tools for the engineering and study of plant systems. (bvsalud.org)
  • A. tumefaciens has flagella that allow it to swim through the soil towards photoassimilates that accumulate in the rhizosphere around roots. (wikipedia.org)
  • Agrobacterium tumefaciens can live independently within soil before infecting its host (McCullen and Binns, 2006). (richmond.edu)
  • Unlike the nitrogen-fixing symbionts, tumor-producing Agrobacterium species are pathogenic and do not benefit the plant. (wikipedia.org)
  • Possible plant compounds that initiate Agrobacterium to infect plant cells: Acetosyringone and other phenolic compounds alpha-Hydroxyacetosyringone Catechol Ferulic acid Gallic acid p-Hydroxybenzoic acid Protocatechuic acid Pyrogallic acid Resorcylic acid Sinapinic acid Syringic acid Vanillin To transfer the T-DNA into the plant cell, A. tumefaciens uses a type IV secretion mechanism, involving the production of a T-pilus. (wikipedia.org)
  • We anticipate that these data will enable users to develop new approaches to in-leaf library development using Agrobacterium transient expression and reliable combinatorial assaying of multiple heterologous proteins in a single plant cell. (bvsalud.org)
  • Economically, A. tumefaciens is a serious pathogen of walnuts, grape vines, stone fruits, nut trees, sugar beets, horse radish, and rhubarb, and the persistent nature of the tumors or galls caused by the disease make it particularly harmful for perennial crops. (wikipedia.org)
  • Here, we analyze the Poisson probability distribution of the T-DNA transfer in leaf pavement cells to determine the MOI for the widely used model system Agrobacterium GV3101/Nicotiana benthamiana. (bvsalud.org)
  • ABC-C6 knockdown has no impact on the attraction of the plant pathogen Agrobacterium tumefaciens , or the plant growth promoting Bacillus subtilis , relative to controls. (biorxiv.org)
  • These isolates were identified using molecular tools like 16s rRNA gene sequences namely Bacillus pumilus AS02, Sphingobacterium thalpophilum AS34, Pseudomonas aeruginosa AS36, Agrobacterium tumefaciens AS38 and Enterobacter aerogenes AS75. (vegetosindia.org)
  • Previous studies have shown that the widely used plant transformation vector Agrobacterium tumefaciens can persist in genetically engineered plants in vitro and in transgenic greenhouse-grown plants, despite the use of counter-selective antibiotics. (ebr-journal.org)
  • We also assayed for A. tumefaciens in transgenic pine and spruce embryogenic tissue and from needles, branches, stems and roots of transformed plants, up to four years post-inoculation. (ebr-journal.org)
  • From these data we conclude that if A. tumefaciens persists in transgenic conifers, it does so beneath our ability to detect it. (ebr-journal.org)
  • Producing composite plants with transgenic roots and nontransgenic stems and buds using Agrobacterium rhizogenes-mediated hairy root transformation is a powerful tool to study root-related biology. (bvsalud.org)
  • Economically, A. tumefaciens is a serious pathogen of walnuts, grape vines, stone fruits, nut trees, sugar beets, horse radish, and rhubarb, and the persistent nature of the tumors or galls caused by the disease make it particularly harmful for perennial crops. (wikipedia.org)
  • This study exposes the link between ABC transporters, root exudate composition, and ex planta interactions with agriculturally and economically relevant rhizosphere organisms, paving the way for an entirely new approach to rhizosphere engineering and crop protection. (biorxiv.org)
  • However, crop domestication has exerted significant and unintended changes to crop root exudate composition, and we know very little about genotype - phenotype linkages that pertain to root exudates and rhizosphere interactions. (biorxiv.org)
  • Establishment and maintenance of mutualistic plant-microbial interactions in the rhizosphere and within plant roots involve several root cell types. (biomedcentral.com)
  • 2006) The role of root exudates in rhizosphere interactions with plants and other organisms. (aimspress.com)
  • Here, we analyze the Poisson probability distribution of the T-DNA transfer in leaf pavement cells to determine the MOI for the widely used model system Agrobacterium GV3101/Nicotiana benthamiana. (bvsalud.org)
  • A. tumefaciens is probably unique among natural plant pathogens in carrying out trans-Kingdom horizontal gene transfer during an infection, and it is this ability that has been widely exploited for creating GM crops, grown on an estimated 134 million hectares worldwide in 2009, and "jumped' another 10 percent in 2010, according to industry-funded International Service for the Acquisition of Agri-biotech Applications (ISAAA) [6]. (farmwars.info)
  • Possible plant compounds that initiate Agrobacterium to infect plant cells: Acetosyringone and other phenolic compounds alpha-Hydroxyacetosyringone Catechol Ferulic acid Gallic acid p-Hydroxybenzoic acid Protocatechuic acid Pyrogallic acid Resorcylic acid Sinapinic acid Syringic acid Vanillin To transfer the T-DNA into the plant cell, A. tumefaciens uses a type IV secretion mechanism, involving the production of a T-pilus. (wikipedia.org)
  • Only root cap and epidermis are in direct contact with the rhizosphere, but plant responses to stimuli also involve internal cell types such as cortex, endodermis, pericycle and vasculature [ 3 ]. (biomedcentral.com)
  • In the presence of opines, A. tumefaciens produces a diffusible conjugation signal called 30C8HSL or the Agrobacterium autoinducer[citation needed]. (wikipedia.org)
  • This study aimed to determine the types mycorrhizal of rhizosphere coffee plants and determine the effectiveness of zeolite media and corn symbiont plant in propagation of VAM. (unud.ac.id)