Reverse transcription and polymerase chain reaction reverse transcriptase polymerase chain reaction. Medical search. Frequent questions

In PCR, a thermostable DNA polymerase is used to amplify target DNA 2-fold with each temperature cycle. (medscape.com)
Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
Finally, nucleotides complementary to the target DNA are added extending each primer by the thermostable DNA polymerase. (medscape.com)
This is the reason why a thermostable DNA polymerase is used like for example the Pfu- or Tag-Polymerase. (hoelzel-biotech.com)

The aim of such a polymerase chain reaction is to amplify the DNA, i.e. genetic material. (hoelzel-biotech.com)
PCR (polymerase chain reaction) tests are a fast, highly accurate way to diagnose certain infectious diseases and genetic changes. (medlineplus.gov)

RNA-1 is 5,449 nucleotides in length and contains one open reading frame (ORF) that encodes for a combined protein that has methyltransferase, helicase, and an RNA-dependent RNA polymerase domains. (wikipedia.org)
Here, DNA polymerase migrates the nucleotides of the DNA single strand piece by piece. (hoelzel-biotech.com)

Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
Real-time quantitative reverse transcription polymerase chain reaction detection of SARS-CoV-2 Delta variant in Canadian wastewater. (cdc.gov)
Viral load and host gene expression were assessed by quantitative reverse-transcriptase polymerase chain reaction. (ubc.ca)

This process is called reverse transcription PCR (rtPCR). (medlineplus.gov)

9. Human telomerase reverse transcriptase mRNA expression assessed by real-time reverse transcription polymerase chain reaction predicts chemosensitivity in patients with ovarian carcinoma. (nih.gov)

Viruses are enriched by antibodies in the PCR microwells, followed by lysis of the virus particles, then reverse transcription of the viral RNA. (wikipedia.org)
The diagnosis of many infectious diseases, both viral and bacterial, may include the use of reverse transcriptase-polymerase chain reaction (RT-PCR). (medscape.com)
The test kit can detectthe "N Gene of SARS COV2 using reverse transcriptase loop-mediated amplification of viral nucleic acid (RT-LAMP). (indiatimes.com)
We aim to understand population level variation of nasopharyngeal ACE2 transcription in people tested for COVID-19 and the relationship between ACE2 transcription and SARSCoV-2 viral load, while adjusting for expression of: (i) the complementary protease, Transmembrane serine protease 2 (TMPRSS2), (ii) soluble ACE2, (iii) age, and (iv) biological sex. (ubc.ca)
Transcription of the transmembrane ACE2 isoform positively correlates, while transcription of the soluble isoform negatively correlates with viral RNA load after adjusting for age, biological sex, and transcription of TMPRSS2. (ubc.ca)

In this study, primers for sPLA 2 -IIA are: forward 5'- GACTCATGACTGTTGTTACAACC-3' and reverse 5'-TCTCAGGACTCTCTTAGGTACTA-3' that amplify a 493 bp fragment, and primers for β-actin are: forward 5'-TGGAGAAGAGCTATGAGCTGCCTG-3' and reverse 5'-GTGCCACCAGACAGCACTGTGTTG-3' that amplify a 289 bp fragment. (medscape.com)

If only HIV-1 genotypic mutation testing is needed, order HIVDR / HIV-1 Genotypic Drug Resistance to Reverse Transcriptase, Protease, and Integrase Inhibitors, Plasma. (testcatalog.org)

Total cellular RNA (1-2 µg) was reverse transcribed with random hexamers using Superscript III reverse transcriptase (Invitrogen, CA) as previously described. (medscape.com)
Semi\quantitative invert transcription\polymerase chain response evaluation GATA5 RNA and cDNA had been made by the producers recommended process using invert transcriptase and arbitrary hexamers from a RevertAid First Strand cDNA Synthesis Package (Fermentas). (isme-la2019.org)

Le test d'anticorps utilisant le dosage d'immunoabsorption par enzyme liée (enzyme-linked immunoassay, ELISA) peut aussi être utilisé pour confirmer l'infection par le VFVR en montrant la présence d'anticorps IgM, qui apparaissent brièvement comme une réponse précoce à une infection récente, et d'anticorps IgG, qui persistent pendant plusieurs années. (cdc.gov)
An enzyme called "DNA polymerase" is used within a biochemical reaction. (hoelzel-biotech.com)
An enzyme called polymerase is added to the sample. (medlineplus.gov)

The development of an effective technique to detect the viruses by using nucleic acid amplification, such as polymerase chain reaction (PCR), improves not only clinical diagnosis but also virologic surveillance of mosquito vectors in the field. (nih.gov)

8. Growth arrest in ovarian cancer cells by hTERT inhibition short-hairpin RNA targeting human telomerase reverse transcriptase induces immediate growth inhibition but not necessarily induces apoptosis in ovarian cancer cells. (nih.gov)
TNF-α and IL-1β initiate the cascade of destructive events, in part, through the activation of transcription factor NF-κB, which in turn induces several proinflammatory genes. (spandidos-publications.com)

The role of two members of the ETS (E26 avian leukemia oncogene) family of transcription factors, ETS-1 and ETS-2, has been investigated in many cancers but has not yet been studied in ocular tumors. (molvis.org)

Rapid simultaneous detection of enterovirus and parechovirus RNAs in clinical samples by one-step real-time reverse transcription-PCR assay. (ox.ac.uk)
To exploit these developments, we developed a real-time one-step reverse transcription-PCR (RT-PCR) for the rapid and sensitive detection of EV and HPeV in clinical specimens. (ox.ac.uk)

As little as 5 microl of homogenate from mosquito pools was used in the reverse transcription (RT) reaction followed by the use of three sets of specific primers for the PCR. (nih.gov)
In the next step, the polymerase can then bind to these primers and start replicating the DNA. (hoelzel-biotech.com)

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). (nih.gov)
This study compares estrogen receptor (ER) and progesterone receptor (PR) measured by local laboratory immunohistochemistry (IHC), central IHC, and central reverse-transcriptase polymerase chain reaction (RT-PCR) using a proprietary 21-gene assay. (elsevierpure.com)
A competitive reverse transcription-PCR assay was developed to quantify the expression of lip genes. (ntnu.edu.tw)

Retroviruses are enveloped RNA viruses defined by their mechanism of replication via reverse transcription to produce DNA copies that integrate into the host cell's genome. (msdmanuals.com)

11. Progesterone regulates human telomerase reverse transcriptase gene expression via activation of mitogen-activated protein kinase signaling pathway. (nih.gov)

1. Eribulin mesylate targets human telomerase reverse transcriptase in ovarian cancer cells. (nih.gov)
Reverse transcriptase (RT) PCR is PCR performed on RNA targets. (medscape.com)
Transcriptome and accessibility analyses suggest reduced expression of B lineage transcription factors (TFs) and targets and loss of D and V antisense transcription in IL-7Rα B cells. (babraham.ac.uk)

13. Suppressor of Ty homolog-5, a novel tumor-specific human telomerase reverse transcriptase promoter-binding protein and activator in colon cancer cells. (nih.gov)
The activation status of leptin signals was measured by Western blotting analysis using a phospho-(Tyr705) signal transducer and activator of transcription 3 (STAT3) antibody. (aspetjournals.org)

To produce a diverse antibody repertoire, immunoglobulin heavy-chain (Igh) loci undergo large-scale alterations in structure to facilitate juxtaposition and recombination of spatially separated variable (V), diversity (D), and joining (J) genes. (babraham.ac.uk)
For further characterization, the coat protein (CP) and the RNA-dependent RNA polymerase (RdRp) genes representing the 3′ and 5′ half of CTV genome, respectively, were amplified from dsRNA by RT-PCR. (ppjonline.org)

The gene encodes a member of the integrin alpha chain family of proteins. (cancerindex.org)
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. (cancerindex.org)

The effects of IL-6 and IL-21 depended on STAT3, a transcription factor required for the differentiation of T(H)-17 cells in vivo. (nih.gov)

5. Human telomerase reverse transcriptase promoter regulation in normal and malignant human ovarian epithelial cells. (nih.gov)

It is noteworthy that a chemical chaperone, which could improve the protein-folding capacity, reversed ER stress-induced leptin resistance. (aspetjournals.org)

Rescue treatment with soluble α-Klotho did not reverse endothelial FGF-23 resistance caused by reduced or absent α-Klotho expression in HAECs. (plos.org)

We applied amplifi cation of the specifi c fusion transcripts by reverse transcriptase-polymerase chain reaction (RT-PCR) in fresh, frozen tumors. (medscimonit.com)

4. RIF1 promotes human epithelial ovarian cancer growth and progression via activating human telomerase reverse transcriptase expression. (nih.gov)

10 genomic copies per reaction. (nih.gov)
method II, 20 copies per reaction). (ox.ac.uk)

Inflammation is a biological reaction to disruption in the normal physiological processes [ 1 ]. (springeropen.com)

Generation of the primary antibody repertoire requires V(D)J recombination of hundreds of gene segments in the immunoglobulin heavy chain (Igh) locus. (babraham.ac.uk)

By including reverse transcriptase and DNA polymerase in the whole process, the procedure can be conducted in a single step. (wikipedia.org)

2. [Relationship between human telomerase reverse transcriptase transcriptional level and telomerase activity in three ovarian cancer cell lines]. (nih.gov)

To better understand how hereditary factors influence metastasis in breast cancer, we characterized NDN-mediated transcription. (nih.gov)
The glucocorticoid receptor (GR) is a member of the nuclear hormone receptor superfamily of ligand-activated transcription factors. (ox.ac.uk)

15. Upstream stimulatory factor (USF) as a transcriptional suppressor of human telomerase reverse transcriptase (hTERT) in oral cancer cells. (nih.gov)

6. p21 WAF1 is involved in interferon-β-induced attenuation of telomerase activity and human telomerase reverse transcriptase (hTERT) expression in ovarian cancer. (nih.gov)

14. Human telomerase reverse transcriptase (hTERT): a target molecule for the treatment of cisplatin-resistant tumors. (nih.gov)

Each analysis contained a series of diluted samples for standard curve purposes, as well as negative template and negative reverse transcriptase control samples. (medscape.com)
Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)

At present, India is using Reverse Transcription Polymerase Chain Reaction (RT-PCR) test for SARS-CoV-2 virus or Corona virus. (indiatimes.com)

Positive stranded RNA virus transcription is the method of transcription. (wikipedia.org)

Findings: Analysis showed no association between age and nasopharyngeal ACE2 transcription in those who tested negative for COVID-19 (P = 0092). (ubc.ca)

10. Telomerase reverse transcriptase promotes epithelial-mesenchymal transition and stem cell-like traits in cancer cells. (nih.gov)

The overexpression of RP105 significantly reversed OGD/R‑induced neuronal injuries, as demonstrated by the reduced release of lactate dehydrogenate and enhanced cellular viability, in addition to decreased inflammation, apoptosis and reactive oxygen species. (spandidos-publications.com)
In addition, it is a chain reaction: the end product of the reaction also serves as the starting point for a further, similar reaction. (hoelzel-biotech.com)

The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 6 subunit. (cancerindex.org)

Interpretation: Nasopharyngeal ACE2 transcription plays a dual, contrasting role in SARS-CoV-2 infection of the upper respiratory tract. (ubc.ca)

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Organisms13

Diseases24

Chemicals and Drugs82

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes69

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Health Care12

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