Antibody-capture enzyme-linked immunosorbent assay (ELISA), serum neutralization tests and antigen-capture detection tests, which all test for the presence of Ebola-specific antigens (immune proteins). (thirdage.com)
The viability of lung epithelial BEAS-2B cells, intracellular production of reactive oxygen species, and the levels of pro-inflammatory cytokines were examined by cell counting kit-8 assays, 2',7'-dichlorofluorescein diacetate (DCF-DA) staining, and enzyme-linked immunosorbent assay, respectively. (bvsalud.org)
We examined mRNA expression using reverse transcriptase-polymerase chain reaction primers for 3 osteopontin isoforms in non-small-cell lung cancer and immortalized bronchial epithelial cell lines, and correlated expression with osteopontin secretion into media detected by enzyme-linked immunosorbent assay. (nih.gov)
Angiogenic properties conferred by osteopontin isoforms were evaluated by transfecting cDNA plasmids specific to each isoform and controls into non-small-cell lung cancer cell lines, H153 and H358 (low endogenous osteopontin) and A549 and H460 (high endogenous osteopontin), analyzing conditioned media on a bovine capillary endothelial platform, and measuring vascular endothelial growth factor levels by enzyme-linked immunosorbent assay. (nih.gov)
Rapid molecular assays are a new type of influenza diagnostic test that use isothermal nucleic acid amplification for viral detection. (cdc.gov)
Our laboratory also performs MSDH-designed assays for the molecular detection of the following infectious agents: Bordetella species, Norovirus, Measles, Mumps, and Shiga-Toxin producing E. coli. (ms.gov)
Limit of blank, limit of detection, linear range, and PCR efficiency were assessed for each of the 12 PCR assays using serial samples dilutions. (biomedcentral.com)
Nineteen national-level public health laboratories performed routine dengue diagnostic assays on a proficiency testing panel consisting of two modules: one containing commercial serum samples spiked with cultured dengue viruses for the detection of nucleic acid and non-structural protein 1 (NS1) (Module A) and one containing human serum samples for the detection of anti-dengue virus antibodies (Module B). A review of logistics arrangements was also conducted. (who.int)
RV detection and genotyping was performed by means of real time and conventional reverse-transcriptase polymerase chain reaction assays, followed by sequence analysis. (ox.ac.uk)
These assays are commercially available for detection of bacterial and viral pathogens, including HIV-1, cytomegalovirus, enteric viruses, Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycobacterium tuberculosis . (medscape.com)
Viral2
Quantitative assays for HCV RNA Tests to detect HCV RNA concentration (viral load) by amplification of viral genetic sequences or by signal amplification. (cdc.gov)
The diagnosis of many infectious diseases, both viral and bacterial, may include the use of reverse transcriptase-polymerase chain reaction (RT-PCR). (medscape.com)
Molecular Diagnostic assays utilize a broad range of techniques that enable the rapid identification of microorganisms at the genetic level. (ms.gov)
Blood and urine samples were tested at icddr,b through rapid diagnostic tests, bacterial culture, and polymerase chain reaction (PCR). (cdc.gov)
Quantitative5
In the present study, we examined five cell lines including three cell lines from pancreatic cancer (SW1990, Capcan-1 and PANC-1) and two cell lines from colon cancer (HCT116 and RKO) for DcR3 expression by reverse transcriptase-polymerase chain reaction (RT-PCR) and quantitative real time PCR. (academicjournals.org)
As summarized in this issue of The ASCO Post , Ivey and colleagues demonstrated that assessing for NPM1- mutated gene transcripts by reverse-transcriptase quantitative polymerase chain reaction assay is a feasible approach for measuring minimal residual disease after acute myeloid leukemia (AML) induction therapy. (ascopost.com)
The test is based on the combined analysis of 12 genes in formalin-fixed, paraffin-embedded (FFPE) tissue by reverse transcription-quantitative real-time PCR (RT-qPCR). (biomedcentral.com)
Primers2
The RTPCR assay was performed by use of tyrosinase, Melan-A, gp100, tyrosinase-related protein 2 (TRP-2), or melanoma antigen-encoding gene B (MAGE-B)- specific primers. (avma.org)
Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
Fourteen brain specimens were positive in reverse transcriptase-polymerase chain reaction assays, confirming the presence of West Nile/Kunjin virus. (cdc.gov)
We evaluated the cost-effectiveness of recurrence score (RS)-guided treatment using 21-gene assay as compared with treatment guided by the Adjuvant! (mcmaster.ca)
A Markov model was developed to compare the cost-effectiveness of treatment guided either by 21-gene assay or by AOL in a 50-year-old woman with lymph node-negative HR-positive breast cancer over a lifetime horizon. (mcmaster.ca)
ICER increased with increasing cost of 21-gene assay and increasing age of patients. (mcmaster.ca)
The 21-gene assay appears cost-effective from a Canadian health care perspective. (mcmaster.ca)
Gene expression values to calculate the EP score were assayed by one-step RT-qPCR using RNA from FFPE tumor tissue. (biomedcentral.com)
Laboratory1
The aim of this study was the analytical validation of the performance characteristics of the assay and its verification in a molecular-pathological routine laboratory. (biomedcentral.com)
Isoforms1
Reverse transcriptase-polymerase chain reaction assays for Ca2+ and K+ channel isoforms may identify susceptibility subgroups with lower resistance to environmental exposures. (cdc.gov)
Immunohistochemistry1
Epidermal proliferation was assessed using immunohistochemistry (Ki67, keratins 6, 16 and 17) and with an in vitro proliferation assay. (vu.nl)
Targets1
Reverse transcriptase (RT) PCR is PCR performed on RNA targets. (medscape.com)
Detect1
Objective -To develop a reverse transcriptase-polymerase chain reaction (RT-PCR) assay to detect canine melanoma-associated antigens (MAAs) and to use this technique to screen aspirates of lymph nodes (LNs) for evidence of metastatic spread of oral malignant melanoma. (avma.org)
Rapid2
1 RIDTs do not include rapid molecular assays. (cdc.gov)
At present, only one rapid molecular assay is FDA-approved for use in the United States. (cdc.gov)
Infection1
West Nile virus infection was confirmed post mortem by use of reverse transcriptase-polymerase chain reaction assay and immunohistochemical staining. (oregonstate.edu)
Expression2
The aim of this study was to analyze changes in the expression of genes coding for enzymes involved in the biosynthesis of glycosaminoglycans (GAGs) chains following the adherence of Candida and Malassezia yeasts to skin cell lines. (actasdermo.org)
Expression of INF-γ, INF-β, IL-1β, IL-2, IL-6, IL-18 in the bursa of Fabricius using a multiplex assay, percentage of bursal weight to body weight (BW: BW), bursal histopathology, and antibody titres against IBDV were compared following oral administration of 'variant' IBDV in groups of birds at day-3 or day-6 post-hatch. (thepoultrysite.com)
Endurance exercise results in DNA damage as detected by the comet assay. (oregonstate.edu)
Sixteen of the 18 laboratories using IgM assays in Module B obtained the correct results as did the 13 laboratories that performed IgG assays. (who.int)
Analysis2
The choice of adequate controls for reverse transcriptase (RT-) PCR analysis has been the focus of a debate pursued in Leukemia over the past 3 years. (nih.gov)
3′-(4, 5 dimethyl-thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and flow cytometric analysis were used to measure cell proliferation and apoptosis, respectively in the human pancreatic cancer Capan-1 cells. (academicjournals.org)
Compounds2
Using this assay, we expected to evaluate the PAR 1 /PAR 2 selectivity of the compounds listed in Table 1 . (aspetjournals.org)
The reaction of compounds 8a-d with hydrazine in ethanol at room temperature took place under nucleophilic substitution which furnished 5-amino-4,6-dioxo-octahydropyrrolo [3,4-b] pyrrole-3-carboxylic acid phenylamides (12a-d). (tu-dortmund.de)
Proteins1
Nitrosamines form by chemical reactions between nitrites and secondary amines or proteins. (biomedcentral.com)
Synthesis1
Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
Abstract1
Worachartcheewan, Apilak Abstract: Pyrrolidines type 2,4-disubstituted (alkyl, aryl or heteroaryl)-6,8-dioxo-3,7-diazabicyclo [3.3.0] octanes (8a-d) were successfully synthesized by an efficient one pot 1,3-dipolar cycloaddition of azomethine ylides (in situ generated from the reaction of aromatic aldehydes and methyl ester of alpha-amino acids) with dipolarophile (N-phenylmaleimide). (tu-dortmund.de)
Cell3
The abilities of the PAR 1 -targeted agents to desensitize or block PAR 1 in the HEK cell assay were compared with their activities in a human platelet aggregation assay. (aspetjournals.org)
Our data illustrate the usefulness of the HEK cell assay for evaluating the PAR 1 /PAR 2 selectivity of PAR-activating agonists. (aspetjournals.org)
Infectious virus was detected by Vero cell culture and plaque assay. (medscape.com)
This survey reviews the issues discussed, and presents current options for appropriate positive controls in RT-PCR assays which are based on the views shared by the majority of participants in the Debate. (nih.gov)
Cells3
We developed a calcium signaling-based assay, using cultured human embryonic kidney cells (HEK), that evaluates simultaneously, the activation/desensitization or blockade of the proteinase-activated receptors, PAR 1 and PAR 2 . (aspetjournals.org)
To this end, we developed a calcium-signaling assay, employing cultured human embryonic kidney cells (HEK293) in which the action of PAR 1 and PAR 2 agonists and antagonists could be evaluated simultaneously. (aspetjournals.org)
Studies using PSA reverse transcriptase--polymerase chain reaction assays and other staining techniques have documented clearly the increased dispersal of cancer cells throughout the body from surgical manipulation. (urologyweb.com)
Furthermore, we used this assay to develop a PAR 1 receptor-activating probe [Ala-parafluoroPhe-Arg-Cha-Cit-Tyr-NH 2 (Cit-NH 2 )], which displays a high degree of specificity for PAR 1 over PAR 2 , and we used the assay to quantitate the ability of trypsin to disarm the activation of PAR 1 by thrombin. (aspetjournals.org)
In both forms of male infertility the ability to undergo an acrosome reaction decreases. (cdc.gov)
Patients1
2 This study demonstrates the value of assessing minimal residual disease in AML by rigorously defining and validating the prognostic performance of an AML minimal residual disease assay in a large cohort of patients uniformly treated on the UK National Cancer Research Institute AML17 trial. (ascopost.com)
Sensitive1
Nested PCR and reverse-transcriptase (RT) PCR are the most sensitive methods. (medscape.com)
Early1
These Ca2+ and K+ channels are involved in early events of acrosome reactions. (cdc.gov)