P130Nuclear ProteinsGenesPhosphorylationMRNAs and proteinsEncodePRB2ViralAntibodyRegulatoryOncogeneInhibitoryCellular proteinsCyclin-dependIntracellularGene productFamilyEpithelial cellsAdenovirus E1A proTranscription factorRecombinant ProteinRegulationDisruptionBiologicalRegulateMolecularCoactivatorNucleusNucleicMRNAKinasesTumor suppressorComplexesBRCA1SubstrateInteractionPhosphataseScopeHumanHumansDomainsBiochemicalTranscriptionalMutant
P13011
- Chief among the intracellular signals is the phosphorylation of proteins belonging to the pocket protein family, such as p107, p130 and retinoblastoma protein (Rb). (bdbiosciences.com)
- The members of the retinoblastoma family of pocket proteins, pRb, p107 and p130, are negative regulators of the cell cycle with tumor suppressor activity and with roles in differentiation. (oncotarget.com)
- Pocket proteins Rb, p107 and p130 play central roles in cell cycle progression and differentiation. (eu.org)
- The LxSxE motif is specific for p107/p130 and is present in LIN52, a component of the DREAM complex. (eu.org)
- A phosphorylation downstream to the core motif acts as a switch that binds to a positively charged pocket only present in p107/p130. (eu.org)
- The LxDLFD motif ( LIG_RB_pABgroove_1 ) mediates binding to a highly conserved deep groove formed in the interface between the A and B subdomains of the Rb, p107 and p130 pocket domains. (eu.org)
- Crk-associated substrate p130(Cas) interacts with nephrocystin and both proteins localize to cell-cell contacts of polarized epithelial cells. (ucdenver.edu)
- Furthermore, our work has shown that epigenetic mechanisms controlled by pRb2/p130, as well as epigenetic events affecting Rb2/130 gene expression itself, play an important role in retinoblastoma and lung cancer formation and progression, and can represent key events in the differentiation of cornea and conjunctiva normal cells. (shro.org)
- pRb family proteins (in particular pRb2/p130) and regulation of estrogen receptors (alpha and beta) gene expression by chromatin remodeling in breast cancer. (shro.org)
- Furthermore, using chromatin immunoprecipitation assays, we show that E2F1 and the pocket protein family members p107 and p130 bind to the AR promoter in vivo. (johnshopkins.edu)
- Pro-proliferative focus on genes cyclinD1 (CCND1) and E2F1 aswell as anti-proliferative goals CDKN1A (p21) PTEN RB1 RBL1 (p107) RBL2 (p130) had been proven as common goals for miR-17 -20 and -106b. (techblessing.com)
Nuclear Proteins2
Genes8
- In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. (wikipedia.org)
- In particular, we will discuss the modifications in intrinsic and extrinsic apoptotic pathways caused by proteins encoded by HPV early genes. (mdpi.com)
- Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (e.g., c-abl and SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein, Rb), nucleolin and others. (bdbiosciences.com)
- Proliferation was characterized by enrichment in genes involved in basic cellular and metabolic processes (transcription, ribosome biogenesis, translation and protein folding), cellular remodelling and autophagy. (biomedcentral.com)
- The variety of keratin genes differentially expressed suggests that these proteins may however have additional functions. (exposed-skin-care.net)
- Genes whose protein products stimulate or enhance the division and viability of cells. (cancerquest.org)
- Genes whose protein products can directly or indirectly prevent cell division or lead to cell death. (cancerquest.org)
- A substantial portion of the regulatory interactions in the higher eukaryotic cell are mediated by simple sequence motifs in the regulatory segments of genes and (pre-)mRNAs, and in the intrinsically disordered regions of proteins. (biomedcentral.com)
Phosphorylation6
- The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27Kip1. (bdbiosciences.com)
- Although viewed as a constitutive housekeeping enzyme in the past, PP2A is a highly regulated phosphatase and is emerging as an important regulator of multiple cellular processes involving protein phosphorylation. (ac.be)
- 1 Reversible protein phosphorylation is an important regulatory mechanism that controls the activities of a myriad of proteins and is thus involved in virtually every major physiological process. (ac.be)
- But advances in the understanding of protein phosphatases make now clear that these enzymes are precisely regulated and are as important as kinases in the regulation of cellular processes involving protein phosphorylation. (ac.be)
- 4 While proteins can be phosphorylated on nine amino acids, serine, threonine and tyrosine phosphorylation are by far the most predominant in eukaryotic cells. (ac.be)
- It is a signal transducing adaptor protein that undergoes tyrosine PHOSPHORYLATION in signaling pathways that regulate CELL MIGRATION and CELL PROLIFERATION. (ucdenver.edu)
MRNAs and proteins1
- This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
Encode2
- Many strikingly miR-17 -20 and -106b had been found to market cell proliferation by raising the intracellular activity of E2F transcription elements even though miR-17 -20 and -106b straight focus on the transcripts that encode because of this proteins family members. (techblessing.com)
- We observe that each gene and its products have a unique set of DNA, RNA or protein motifs that encode a regulatory program to define the logical circuitry that guides the life cycle of these biomolecules, from transcription to degradation. (biomedcentral.com)
PRB21
- In this context, we have suggested that the silencing of estrogen receptor alpha (ER-α) gene during breast cancer progression, as well as of p73 gene in osteosarcoma, could arise from epigenetic events mediated by pRb2/130 (and perhaps by pRb1/105 and p107) in association with specific enzymes involved in the chromatin remodeling. (shro.org)
Viral1
- The LxCxE motif binds to all pocket proteins and is present in chromatin regulators such as HDAC and KDM5A and in viral proteins. (eu.org)
Antibody1
- Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. (bdbiosciences.com)
Regulatory6
- Protein Phosphatase 2A (PP2A) is a widely expressed family of protein phosphatases made of a core dimer, composed of a catalytic (C) subunit and a structural (A) subunit, in association with a third variable regulatory (B) subunit. (ac.be)
- An interferon regulatory factor that is induced by INTERFERONS as well as LMP-1 protein from EPSTEIN-BARR VIRUS. (umassmed.edu)
- It contains an N-terminal RING FINGER DOMAIN and is a PROTEIN PHOSPHATASE 1 regulatory subunit. (jefferson.edu)
- Our interest is to carry out the complex network of signals by which this family of proteins regulates cellular proliferation, survival and differentiation, and to disclose the mechanisms responsible for the regulatory disruption of these functions in the neoplastic process. (shro.org)
- A family of endogenous regulatory proteins that associate with RETINOBLASTOMA PROTEIN via a specific high-affinity binding domain. (bvsalud.org)
- Finally, we contrast the regulatory properties of protein motifs and the regulatory elements of DNA and (pre-)mRNAs, advocating that co-regulation, co-operativity, and motif-driven regulatory programs are common mechanisms that emerge from the use of simple, evolutionarily plastic regulatory modules. (biomedcentral.com)
Oncogene4
- Proto-Oncogene Proteins c-mdm2" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
- This graph shows the total number of publications written about "Proto-Oncogene Proteins c-mdm2" by people in this website by year, and whether "Proto-Oncogene Proteins c-mdm2" was a major or minor topic of these publications. (umassmed.edu)
- Below are the most recent publications written about "Proto-Oncogene Proteins c-mdm2" by people in Profiles. (umassmed.edu)
- Crk-associated substrate was originally identified as a highly phosphorylated 130 kDa protein that associates with ONCOGENE PROTEIN CRK and ONCOGENE PROTEIN SRC. (ucdenver.edu)
Inhibitory1
- The activity of CDKs is controlled by their binding to coactivator subunits termed Cyclins, as well as by CDK inhibitory proteins termed CKIs. (intechopen.com)
Cellular proteins2
- Cloning of cDNAs for cellular proteins that bind to the retinoblastoma gene product. (wikigenes.org)
- 2 Protein phosphatase 2A (PP2A) is a very abundant - it accounts for as much as 1% of total cellular proteins - ubiquitous and remarkably conserved enzyme. (ac.be)
Cyclin-depend1
- A group of cell cycle proteins that negatively regulate the activity of CYCLIN / CYCLIN-DEPENDENT KINASE complexes. (nih.gov)
Intracellular1
- Although intracellular signal transduction is often portrayed as a protein kinase 'domino effect', the counterbalancing function of phosphatases, and thus the control of phosphatase activity, is equally relevant to proper regulation of cellular function. (ac.be)
Gene product1
- The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. (wikipedia.org)
Family10
- this family of proteins is hereafter called EBF) are a group of DNA-binding transcription factors with the basic helix-loop-helix (bHLH) domain. (aacrjournals.org)
- Here we show a novel function for Retinoblastoma family member, p107 in controlling stem cell expansion in the mammalian brain. (rupress.org)
- In this work we studied the relative contribution of the retinoblastoma family members to the regulation of Sox2 expression. (oncotarget.com)
- The members from the large keratin family of cytoskeletal proteins are expressed in a carefully regulated tissue- and differentiation-specific manner. (exposed-skin-care.net)
- The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shockproteins (HSP 70). (exposed-skin-care.net)
- Keratins are a large family of proteins which form the intermediate filament (IF) cytoskeleton of epithelial cells and their appendages hairs and nails (reviewed in references 8 and 15). (exposed-skin-care.net)
- A family of cell cycle proteins containing ANKYRIN REPEATS that are specific inhibitors of cyclin D-dependent kinases. (nih.gov)
- Our specific research has concentrated on the mechanisms regulated by pRb-family proteins and governing the neoplastic processes. (shro.org)
- Role of pRb family proteins in lung cancer formation and progression. (shro.org)
- Members of this family of proteins are often found associated with histone-modifying enzymes and protein complexes that regulate gene expression. (bvsalud.org)
Epithelial cells1
- We show that exogenous expression of E2F1 significantly inhibited AR mRNA and AR protein levels in prostate epithelial cells. (johnshopkins.edu)
Adenovirus E1A pro2
- Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 Large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. (wikipedia.org)
- The adenovirus E1A protein mimics the LxDLFD motif and competes with E2F for binding to Rb, promoting E2F activation and cell proliferation. (eu.org)
Transcription factor1
- Cao L, Faha B, Dembski M, Tsai LH, Harlow E, Dyson N. Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F. (bdbiosciences.com)
Recombinant Protein1
- E2F3( AAH16847, 1 a.a. - 134 a.a.) recombinant protein with GST. (novusbio.com)
Regulation3
- In the past, most of the attention was focused primarily on protein kinases and on their regulation, mainly because phosphatases were then viewed as simple housekeeping enzymes. (ac.be)
- The most studied function of the Rb protein is in the regulation of cell cycle progression at the G1/S boundary ( Giacinti,2006 ). (eu.org)
- Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
Disruption2
- Pocket proteins play essential roles in cell cycle progression, quiescence and differentiation, and their functional disruption is associated with human cancer. (eu.org)
- Activation of E2F transcription factors, through disruption of the retinoblastoma (Rb) tumor-suppressor gene, is a key event in the development of many human cancers. (johnshopkins.edu)
Biological1
- This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. (novusbio.com)
Regulate1
- The mutant proteins often retain some of their capabilities but are no longer sensitive to the controls that regulate the normal form of the protein. (cancerquest.org)
Molecular2
- Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
- Using different approaches we also found that the molecular system where these keratins modulate cell development appears to be associated with pRb as well as the molecular equipment controlling cell routine development during G1 which regarding K10 this function resides in the nonhelical termini from the protein. (exposed-skin-care.net)
Nucleus4
- These include changes in the plasma membrane (such as loss of membrane symmetry and loss of membrane attachment), a condensation of the cytoplasm and nucleus, protein cleavage, and internucleosomal cleavage of DNA. (bdbiosciences.com)
- In normal cells the BRCA1 protein is localized in the nucleus, whereas in the majority of breast cancer cell lines and in malignant pleural effusions from breast cancer patients, it is localized mainly in the cytoplasm. (jefferson.edu)
- Proteins found in the nucleus of a cell. (uams.edu)
- Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. (uams.edu)
Nucleic1
- Cellular nucleic acid-binding protein is essential for type I interferon-mediated immunity to RNA virus infection. (umassmed.edu)
MRNA1
- ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
Kinases1
- Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. (bdbiosciences.com)
Tumor suppressor2
- Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. (wikipedia.org)
- Its ability to ubiquitinate p53 is regulated by TUMOR SUPPRESSOR PROTEIN P14ARF. (umassmed.edu)
Complexes1
- After translation, where is a protein localised, and what complexes should it join? (biomedcentral.com)
BRCA11
- Below are the most recent publications written about "BRCA1 Protein" by people in Profiles. (jefferson.edu)
Substrate3
- PP2A/B55α substrate recruitment as defined by the retinoblastoma-related protein p107. (academic-accelerator.com)
- Crk-Associated Substrate Protein" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ucdenver.edu)
- Below are the most recent publications written about "Crk-Associated Substrate Protein" by people in Profiles. (ucdenver.edu)
Interaction1
- Faha B, Ewen ME, Tsai LH, Livingston DM, Harlow E. Interaction between human cyclin A and adenovirus E1A-associated p107 protein. (bdbiosciences.com)
Phosphatase1
- Functional proteomics identifies protein-tyrosine phosphatase 1B as a target of RhoA signaling. (ucdenver.edu)
Scope1
- Scope includes mutations and abnormal protein expression. (cancerindex.org)
Human3
- The effects of POP inhibition and knockdown on the proliferation of cultured human estrogen receptor-positive (ER+) MCF7 and T47D, and ER-negative (ER-) MDA-MB-231 breast cancer cell lines and the MCF12A non-tumorigenic epithelial cell line were tested by analyzing their influence on cell proliferation (WST-1 assay), cell viability (trypan blue exclusion assay), and cell cycle arrest (cell cycle analysis, cell cycle regulator proteins expression). (cancerindex.org)
- Inactivation of Rb may contribute to many human malignancies including familial retinoblastoma, small-cell lung carcinomas, cervical carcinomas, prostate carcinomas, breast carcinomas, and some forms of leukaemias ( Burkhart,2008 ). (eu.org)
- The other project involves the study of a single nucleotide polymorphism (SNP) in the human p53 gene that results in either arginine (R) or proline (P) at position 72 of the p53 protein. (mdanderson.org)
Humans1
- Retinoblastoma-like 1 (p107), also known as RBL1, is a protein that in humans is encoded by the RBL1 gene. (wikipedia.org)
Domains1
- Variations in these nonhelical end domains largely account for the differences between individual keratin proteins. (exposed-skin-care.net)
Biochemical1
- These proteins are subdivided according to biochemical criteria into two subfamilies: type I or acidic keratins and type II or neutral-basic keratins. (exposed-skin-care.net)
Transcriptional2
- A ubiquitously expressed sequence-specific transcriptional repressor that is normally the target of signaling by NOTCH PROTEINS. (harvard.edu)
- Deleterious, protein-altering variants in the transcriptional coregulator ZMYM3 in 27 individuals with a neurodevelopmental delay phenotype. (uams.edu)
Mutant1
- GRP78 is a resident protein of the endoplasmic reticulum (ER) and mayassociate transiently with a variety of newly synthesized secretory and membrane proteins orpermanently with mutant or defective proteins that are incorrectly folded, thus preventing theirexport from the ER lumen. (exposed-skin-care.net)