The two inhibitors cerulenin and 5-(tetradecyloxy)-2-furoic acid (TOFA) were used to modulate fatty acid synthesis. (uni-goettingen.de)
Immunologic and confocal microscopic evidence also suggested a transient but significant stimulation of tyrosine phosphorylation of Cav-1 in β-cells briefly (for 15 min) exposed to IL-1β that was markedly attenuated by three structurally distinct inhibitors of protein tyrosine phosphorylation. (diabetesjournals.org)
Finally, blocking lipogenesis with fatty acid synthase inhibitors (cerulenin and C75) and siRNA-mediated silencing of SREBP1 and ATP citrate lyase (ACL) significantly attenuated GSK3 inhibition-induced senescence. (koreamed.org)
2010). Blocking of lipogenesis with siRNA or FAS-specific inhibitors cerulenin and C75 attenuates the cell senescence. (webmedcentral.com)
FASN3
We observed a highly up-regulated fatty acid synthase (FASN) level in A549CisR and H157CisR cells compared to parental cells and the up-regulation of FASN was also detected in A549P and H157P cells after short time treatment with cisplatin, suggesting that the high level of FASN in cisplatin-resistant cells may be from the accumulated cellular responses during cisplatin-resistance developmental process. (oncotarget.com)
We next investigated whether the inhibition of FASN by using a specific FASN inhibitor, cerulenin, can influence growth and EMT/metastatic potential of A549CisR and H157CisR cells. (oncotarget.com)
Cerulenin, a fatty acid synthase (FASN) natural inhibitor, and Atorvastatin, a competitive inhibitor of the mevalonate pathway, have been used to test the cytotoxic effects on PDAC CSCs. (atlasofscience.org)
SiRNA-mediated1
Overexpression of an inactive mutant of Cav-1 lacking the tyrosine phosphorylation site (Y14F) or an siRNA-mediated Cav-1 knock down also resulted in marked attenuation of IL-1β-induced iNOS gene expression and NO release from these cells, thus further implicating Cav-1 in this signaling cascade. (diabetesjournals.org)
Fatty acid2
Our findings suggested an acute reduction of fatty acid or cholesterol production that could target specific stemness features. (atlasofscience.org)
Certain experiments have also suggested the presence of "fatty acid transport tunnels" within the beta-ketoacyl-ACP synthase domain that lead to one of many "fatty acid cavities", which essentially acts as the active site. (wikipedia.org)
Lipogenesis1
We suggested a more plausible explanation based on the results of our recent study, which found that enhanced lipogenesis in response to expression of sterol regulatory element binding protein 1 (SREBP1, also known as SREBF1) is involved in the increase in the organellar mass observed in senescent cells [ 10 ]. (koreamed.org)
Mechanism1
As a result, the regulation of fatty acids suggests a possible adaptation mechanism of neurons to hypoxic conditions. (uni-goettingen.de)
Reduction2
No significant tumor regression was detected at the end of cerulenin treatment, but IHC staining showed higher expression of EMT/metastasis markers in H157CisR cell-derived tumors than H157P cell-derived tumors, and showed dramatic reduction of these markers in tumor tissues of cerulenin-treated mice, confirming the in vitro results. (oncotarget.com)
Acetic acid, a byproduct formed during yeast alcoholic fermentation, is the main component of volatile acidity (VA). When present in high concentrations in wine, acetic acid imparts an undesirable 'vinegary' character that results in a significant reduction in quality and sales. (oregonstate.edu)
Subsequent1
Here we provide the first evidence to suggest that tyrosine phosphorylation of Cav-1 and subsequent interaction among members of the Ras signaling pathway within the membrane lipid microdomains represent early signaling mechanisms of IL-1β in β-cells. (diabetesjournals.org)
Cellular2
In the context of a stroke, a cascade of reactions is induced at the cellular level, resulting in inflammation and apoptosis. (uni-goettingen.de)
An increase in the mass of any specific cellular compartment has been suggested as a potential cause of senescence. (koreamed.org)
Presence2
By adjusting the expression strategy, the optimized engineered strain produced pinosylvin at 153.7 ± 2.2 mg/L with an extremely high yield of 1.20 ± 0.02 mg/mg cinnamic acid in the presence of cerulenin, which is 83.9% ± 1.17 of the theoretical yield. (biomedcentral.com)
Emerging insights have suggested the presence of a small subpopulation of cells, termed as cancer stem cells (CSCs), which are responsible for tumour initiation, formation and progression in several types of cancer, including pancreatic tumour. (atlasofscience.org)
Effective2
The massive worldwide expansion of antimicrobial resistance, as well as a lack of effective and innovative medicines against these pathogens, may result in economic trouble and substantial morbidity in the future. (springeropen.com)
Cerulenin is an effective blocker of the TonEBP actions. (biomedcentral.com)
Expression2
The expression of Ptr4CL4 drastically improved the production of pinosylvin (42.5 ± 1.1 mg/L), achieving values 15.7-fold higher than that of Ptr4CL5 (another 4-Coumarate-CoA ligase from Populus trichocarpa ) in the absence of cerulenin. (biomedcentral.com)
increased expression of glycolytic and PPP enzymes has been also confirmed by metabolomic results. (atlasofscience.org)
Natural1
To address this notion, known natural Epoxide compounds, Cerulenin and Fosfomycin were examined for QSI activity for the agr and fsr systems in addition to in silico and SAR studies. (springeropen.com)
Block2
From a semi-large-scale screening thus far carried out, two Epoxide compounds, Ambuic acid and Synerazol, have been found to efficiently block agr and fsr QS systems, suggesting that the Epoxide group is involved in the mode of action of these QSIs. (springeropen.com)
Cerulenin was used to block the NF-κB cofactor function of TonEBP. (biomedcentral.com)
Production1
When integrated into the genome of a haploid wine strain, the mutated YAP1 alleles partially reproduced the low-VA production phenotype of the diploid cerulenin-resistant strains, suggesting that YAP1 might play a role in (regulating) acetic acid production during fermentation. (oregonstate.edu)
Play1
It is well established that inflammatory cytokines such as interleukin-1β (IL-1β) play a major regulatory role in the selective destruction of insulin-producing β-cells, resulting in the onset of type 1 diabetes ( 1 - 3 ). (diabetesjournals.org)
Mouse1
The in vitro result was further investigated in orthotopic xenograft mouse models established with luciferase-tagged H157P and H157CisR cells. (oncotarget.com)
Shown1
Previously, it has been shown that saké yeast strains resistant to the antifungal cerulenin produce significantly lower levels of VA. In this study, we used a classical mutagenesis method to isolate a series of cerulenin-resistant strains, derived from a commercial diploid wine yeast. (oregonstate.edu)
Search1
There are more than one record in the search results.Please specify issue. (go.jp)
Inhibition3
siRNAi for FASN did not culminate in apoptosis, and FASN inhibitors treatment did not alter free fatty acids content in the non-tumorigenic cells, as verified by mass spectrometry, suggesting that cerulenin or orlistat induces apoptosis independent on FASN inhibition. (unicamp.br)
Respiratory inhibition after cerulenin or orlistat treatment, respectively, was restricted to the oxidation of NADH-linked substrates (39.9 or 60,8%) and succinate (45.8 or 51.8%) and was not significant when mitochondria were respiring on the complex IV substrate, N,N,N? (unicamp.br)
The decreased synthesis in rat hepatocytes was predominantly the result of potent inhibition of the production of fl-muricholic acid. (docksci.com)
Treatment4
Second, we demonstrated that treatment with the FAS inhibitor, cerulenin (Cer), significantly decreased meningioma cell survival in vitro. (wustl.edu)
Fourth, we demonstrated that Cer treatment of mice bearing meningioma xenografts resulted in significantly reduced tumor volumes associated with increased meningioma cell death. (wustl.edu)
Collectively, our data suggest that the increased FAS expression in human meningiomas represents a novel therapeutic target for the treatment of unresectable or malignant meningioma. (wustl.edu)
Cerulenin or orlistat treatment decreased cells proliferation, accompanied by increased amounts of the tumor suppressor protein p21WAF1/Cip1, as well as induced apoptosis, but not necrosis, in melan-a cell line. (unicamp.br)
Data1
RESULTS: Of the 748 patients (88.5%) with available data, the incidence of VTE was 14.8% (111/748). (bvsalud.org)