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  • Abstract
  • RNA methylation and diseases: experimental results, databases, Web servers and computational models Chen, Xing;Sun, Ya-Zhou;Liu, Hui;Zhang, Lin;Li, Jian-Qiang;Meng, Jia 2017-11-18 00:00:00 Abstract Ribonucleic acid (RNA) methylation is a type of posttranscriptional modifications occurring in all kingdoms of life. (deepdyve.com)
  • synthesis
  • They are located in the nucleolus and the Cajal bodies of eukaryotic cells (the major sites of RNA synthesis), where they are called scaRNAs (small Cajal body-specific RNAs). (wikipedia.org)
  • In eukaryotes, the temporal and spatial separation of mRNA synthesis from translation allows each newly made RNA to be subjected to extensive quality control before it ever encounters a ribosome ( Palazzo and Akef, 2012 ). (frontiersin.org)
  • Suppression of RNA synthesis in lymphocytes by inhibitors of proteolytic enzymes. (naver.com)
  • During one-step growth, synthesis of both positive- and negative-strand JEV RNAs was delayed by the knockdown of hnRNP A2. (asm.org)
  • Total RNA
  • Total RNA was tested from strains carrying Cas9 vector alone (lanes 1 and 2), unc-119 sgRNA vector alone (lanes 3 and 4), and both vectors (lanes 5 and 6) with primers specific for Cas9 (top panel) or unc-119 sgRNA (bottom panel). (nih.gov)
  • Total RNA was isolated by miRNeasy kit. (bmj.com)
  • Deep sequencing technology has enabled discovery of a novel 16-nt RNA sequence in total RNA from human brain that we propose is derived from RNU1, an RNA component of spliceosome assembly. (nih.gov)
  • gene
  • Results so far indicate dramatic improvements in both cystic fibrosis and muscular dystrophy sufferers, although it is only suitable for those disorders caused by the presence of a premature stop sign in a gene sequence, as a result of a mutation. (innovations-report.com)
  • Recent research indicate that siRNA-induced transcriptional gene silencing through DNA methylation occur in various human cell types [ 14 , 15 ], and small nuclear RNAs (e.g., 7SK and U6) have been downregulated by ectopic siRNA activity [ 16 ]. (hindawi.com)
  • Our results suggest the existence of an epigenetic mechanism of miRNA-directed transcriptional gene silencing (TGS) in mammalian cells. (pnas.org)
  • Small noncoding RNAs regulate epigenetic gene silencing pathways that are well-conserved in eukaryotes ( 1 , 2 ). (pnas.org)
  • In recent years, the idea that dynamic RNA methylation plays active roles in gene regulation has been intensively studied. (deepdyve.com)
  • Gene processing control loops suggested by sequencing, splicing, and RNA folding. (nih.gov)
  • In cotransfection studies, SR1848 reduced LRH-1-dependent expression of a reporter gene and in cells that endogenously express LRH-1 dose dependently reduced the expression of cyclin-D1 and -E1, resulting in inhibition of cell proliferation. (sigmaaldrich.com)
  • eukaryotic
  • Spliceosomes are a major component of an integral step in eukaryotic precursor messenger RNA maturation. (wikipedia.org)
  • Eukaryotic cells contain a set of low molecular weight nuclear RNA's. (sciencemag.org)
  • This new understanding of the determinants of nuclear retention and cytoplasmic export provides a deeper understanding of how information flow is regulated in eukaryotic cells. (frontiersin.org)
  • genetic
  • RNA, the transporter of genetic information within the cell, has emerged from the shadow of DNA to become one of the hottest research areas of molecular biology, with implications for many diseases as well as understanding of evolution. (innovations-report.com)
  • Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants. (nih.gov)
  • cellular
  • These observations point to an important role of RNA methylation in cellular process and call for this area to be further studied from both theory and application of biomedicine. (deepdyve.com)
  • expression
  • They use a construct encoding a splice-correcting U7 small nuclear RNA, which results in a vector of 650 base pairs (bp), as compared to a conventional 3600 bp plasmid carrying the same expression cassette. (twisterbiotech.com)
  • These data suggest that overexpression of miR-155 and miR-34a in CD14 cells can result in downregulation of AXL expression which in turn can lead to the overproduction of TNFα. (bmj.com)
  • cytoplasmic
  • Two nuclear extracts and two cytoplasmic extracts from hNP1 human neural progenitor cells were assayed for the 16-nt sequence S . The two nuclear Rn values at 17 PCR cycles were about 1.7 versus the two cytoplasmic Rn values about 1.0. (nih.gov)
  • Two types of negative controls were not detected in both nuclear and cytoplasmic fractions (see text). (nih.gov)