• In molecular biology, restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA. (wikipedia.org)
  • Optical mapping is a genomic technology, pioneered by David Schwartz, which can map the location of restriction sites along a genomic chromosome. (umd.edu)
  • Large regions of genomic colinearity have been demonstrated among grass species by recombinational mapping, but the degree of chromosomal conservation at the sub-centimorgan level has not been extensively investigated. (pnas.org)
  • Genomic DNA samples of HA370 and HA372, the parents of the F2 population used to build the map, were screened for AFLPs using 42 primer combinations and RFLPs using 136 cDNA probes (RFLP analyses were performed on DNA digested with EcoRI, HindIII, EcoRV, or DraI). (usda.gov)
  • Genomic 'walking' between EcoRI and BamHI lambda genomic libraries enabled overlapping neighbouring clones to be identified and mapped. (unife.it)
  • How could you identify clones that contained opposite orientations of fragment a without sequencing the DNA or using restriction enzymes? (sdsu.edu)
  • In our project we plan to rely on the complementary strengths of mapping and sequencing data to help improve genome assembly. (umd.edu)
  • Restriction Mapping and DNA Sequencing. (pnas.org)
  • We used long-read sequencing augmented by transcriptomics, optical and genetic mapping along with shared synteny from closely related fish species to derive a chromosome-level assembly with a contig N50 size over 1 Mb and scaffold N50 size over 25 Mb that span ~90% of the genome. (plos.org)
  • In our study, we employed long read sequencing combined with genetic and optical mapping, and syntenic information to produce a chromosomal level assembly. (plos.org)
  • T oday, it is difficult to imagine a time when our laboratory freezers were not well stocked with restriction enzymes, when DNA sequencing was not possible, or when genes were only accessible to the geneticists and could not be simply cloned out by recombinant DNA technology. (pnas.org)
  • The thesis consists of three separate studies;In the first study, quantitative trait loci (QTL) for seed size, protein and oil content and for the content of the fatty acids palmitate, stearate, oleate, linoleate and linolenate were mapped. (iastate.edu)
  • Tuan D, Biro PA, De Riel JK, Lazarus H, Forget BG (1979) Restriction endonuclease mapping of the human γ-globin gene loci. (springer.com)
  • donovani gDNA was performed to generate a restriction map of the LdLIP3 gene loci and to determine its copy number in the genome. (jyi.org)
  • To this point, association and quantitative trait loci (QTL) mapping approaches in this species have relied on relatively small numbers of genetic markers. (g3journal.org)
  • In maize, the sh2 and a1 loci have been cloned on a single YAC, and map about 140 kb apart ( 11 ). (pnas.org)
  • Abstract This document describes an Extensible Provisioning Protocol (EPP) extension mapping for the provisioning and management of Domain Name System security extensions (DNSSEC) for domain names stored in a shared central repository. (ietf.org)
  • The purpose of this thesis was to map traits of interest to soybean (Glycine max (L.) Merr. (iastate.edu)
  • The DNA Restriction Mapping Model simulates the cutting of a DNA molecule at each occurrence of the sequence GTGCAC or GTTAAC, turning the DNA strand into several smaller strands. (compadre.org)
  • These fragments can then be pieced together to create a map of the original DNA molecule, a process that is called Restriction Mapping. (compadre.org)
  • Following this work, Anantharaman focused his attentions into the field of biostatistics and the application of Bayesian methods to the analysis of single molecule Optical Mapping technologies. (wikipedia.org)
  • In a recent survey commissioned by IATA regarding concerns people had about air travel post-crisis, more than 80% of travelers said they are as concerned about potential quarantine restrictions as they are about actually catching the virus during travel. (asiatraveltips.com)
  • Restriction endonuclease mapping of cellular DNA has been used to identify chromosomes that carry the mutant Hb Presbyterian β-globin genes in a family with individuals heterozygous for this disease. (springer.com)
  • Phillips JA, Pawny SR, Kazazian HH Jr, Boehm CO, Scott AF, Smith KO (1980) Prenatal diagnosis of sickle cell anemia by restriction endonuclease analysis: Hind III polymorphisms in γ-globin genes extend test applicability. (springer.com)
  • DNA mapping is a common procedure used to determine the location of genes. (edvotek.com)
  • It was Nathans who made the key intuitive leap and then went on to demonstrate not only that the resulting fragments could be used to produce a physical map of SV40 ( 3 ), but also that this physical map allowed the mapping of the origin of replication ( 4 ) and the location of SV40 genes ( 5 ). (pnas.org)
  • Rectal and systemic CD8 + CTL clones established in 1 vaccine recipient revealed similar Env-specific responses and MHC restriction. (jimmunol.org)
  • Google Maps Platform Customers received last week an email communication from Google that starting from June 11, 2019 Google will be enforcing API key restrictions to improve security. (localyse.eu)
  • Please review your affected API keys and adjust their API restrictions before June 11, 2019 if you are using API keys for Google Maps Platform services. (localyse.eu)
  • Please review your affected API keys, and adjust their API restrictions before June 11, 2019, if you are using API keys for these services. (localyse.eu)
  • As of September 16, 2019, we began to enforce API key restrictions for API keys used to call the Places Library in the Maps JavaScript API. (google.com)
  • In that paper, Kathleen Danna and Daniel Nathans of Johns Hopkins University (Baltimore) showed for the first time that the restriction enzyme called "endonuclease R," discovered by Hamilton Smith and Kent Wilcox ( 2 ), could be used to produce specific fragments of simian virus 40 (SV40) DNA. (pnas.org)
  • glycinea Kuan and Erwin were mapped with RFLP markers in the second study. (iastate.edu)
  • The mapping was conducted by first screening, with RFLP markers, a series of near isogenic lines (NILs). (iastate.edu)
  • Linkage was found between RFLP markers and Rps1, Rps2, Rps3, Rps4 and Rps5;A variant for the A[subscript]4 polypeptide of the glycinin storage protein was mapped and genetically analyzed in the third study. (iastate.edu)
  • The variant was identified in PI 468916, the G. soja parent of the population being used to form a public RFLP map. (iastate.edu)
  • The individuals in the population used to form the RFLP map were evaluated for the segregation of the A[subscript]4 polypeptide. (iastate.edu)
  • This segregation data was integrated with the RFLP segregation data set and was used to genetically map Gy4, the gene that encodes A[subscript]4. (iastate.edu)
  • The integrated map was built by genotyping 296 AFLP and 104 RFLP markers on 180 HA370 x HA372 F2 progeny (the AFLP marker assays were performed using 18 primer combinations). (usda.gov)
  • The HA370 x HA372 map integrated 14 of 17 linkage groups from two independent RFLP maps. (usda.gov)
  • Three linkage groups were devoid of RFLP markers from one of the two maps. (usda.gov)
  • Two recent studies have demonstrated microcolinearity between rice chromosomal DNA on yeast artificial chromosomes (YACs) and fine scale recombinational maps in the Triticeae ( 9 , 10 ). (pnas.org)
  • Optical mapping data can help remove many of the incorrect options, thus allowing us to more quickly and accurately identify the correct path through the graph. (umd.edu)
  • defines the class as mapping to non-relational data. (eclipse.org)
  • Google Maps keynote "What can you do with intelligent data for your city or community? (localyse.eu)
  • maps a class to an XML document, this can be used with XML data-stores, XML files, XML messaging systems, and other XML systems. (eclipse.org)
  • This can be used to map to key/value stores, JSON databases, and other structured data systems. (eclipse.org)
  • This would produce the following mapped data. (eclipse.org)
  • Maps containing both fragments-length data and clone-end data are maintained for each restriction enzyme, and sychronization between two such maps is achieved by requiring them to have "compatible" clone-end map projections. (wustl.edu)
  • Bangladesh poverty maps showing national estimates for wealth index (A), progress out of poverty index (B), and household income (C). Maps were generated using mobile data, satellite data, and Bayesian geostatistical models. (eurekalert.org)