In molecular biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, populations, or species or to pinpoint the locations of genes within a sequence. (wikipedia.org)
In RFLP analysis, a DNA sample is digested into fragments by one or more restriction enzymes, and the resulting restriction fragments are then separated by gel electrophoresis according to their size. (wikipedia.org)
The results of the Human Genome Project have largely replaced the need for RFLP mapping, and the identification of many single-nucleotide polymorphisms (SNPs) in that project (as well as the direct identification of many disease genes and mutations) has replaced the need for RFLP disease linkage analysis (see SNP genotyping). (wikipedia.org)
Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA . (microbenotes.com)
Simply, the variations in the restriction DNA fragments length between individuals of a species is called RFLP. (microbenotes.com)
An RFLP occurs when the length of a detected fragment varies between individuals. (microbenotes.com)
RFLP analysis is carried out to detect differences in pattern to confirm polymorphisms. (microbenotes.com)
To detect the K540E polymorphism in pfdhps, the restriction fragment length polymorphism (RFLP) was performed. (cdc.gov)
Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, we tested the genotype distribution of 100 migraine patients in comparison with 120 healthy migraine-free controls from the same geographical region. (scirp.org)
Restriction fragment length polymorphism (RFLP) and sequencing results indicated only C588T polymorphism in the studied subjects. (hindawi.com)
The aim of this study was to detect carriers of CVM in Holstein population using Polymerase Chain Reaction- Primer-introduced restriction analysis and Restriction Fragment Length Polymorphism (PCR-PIRA and RFLP) methods. (researchgate.net)
A chloroplast DNA restriction fragment length polymorphism (RFLP) and its relationship with cone morphology was studied in a sympatric population of lodgepole pine and jack pine. (researchgate.net)
Polymerase chain reaction/restriction fragment length polymorphism technique (PCR-RFLP) was conducted to determine the genotypes. (medsci.org)
Polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the ribosomal RNA gene internal transcribed spacer-1 (ITS1) was used. (who.int)
Single-nucleotide po3
Two coding single nucleotide polymorphisms (SNPs) supposedly affect their functions: GSTO1*C419A (rs4925) causing alanine to aspartate substitution (*A140D) and GSTO2*A424G (rs156697) causing asparagine to aspartate substitution (*N142D), and have been associated with several neurodegenerative diseases and cancers. (cancerindex.org)
The aim of this study was to identify any impact of CYP2A13 single-nucleotide polymorphisms (SNPs) on CYP2A13 expression in human lung. (aspetjournals.org)
To date, more than 20 single-nucleotide polymorphisms (SNPs) have been identified in CYP2A13 (Zhang et al. (aspetjournals.org)
Alleles2
A restriction fragment length polymorphism is defined by the existence of alternative alleles associated with restriction fragments that differ in size from each other. (microbenotes.com)
Comprised of DNA fragments that represent common alleles at a locus. (ojp.gov)
Gene polymorphisms2
Methods: This case-control study conducted in Srinagar, Kashmir (North India) between 2013 and 2015 was designed to evaluate risk induced due to MTH-FR 677C>T gene polymorphisms to contribute in susceptibility for migraine in Kashmir population (North India). (scirp.org)
Our study has not shown a significant association between MTHFR gene polymorphisms and breast cancer risk. (medsci.org)
Genetic2
Each fragment length is considered an allele, whether it actually contains a coding region or not, and can be used in subsequent genetic analysis. (wikipedia.org)
CYP2A13 genetic polymorphisms may be associated with interindividual differences in susceptibility to tobacco-related tumorigenesis, because the resultant variations in CYP2A13 expression and metabolic activity can significantly alter the extent of NNK metabolic activation in human lung. (aspetjournals.org)
Susceptibility2
The purpose of this case-control research is to determine if GABRG2 polymorphisms contribute to IGE susceptibility and antiepileptic drug resistance in Pakistani population. (hindawi.com)
Two common polymorphisms in the MTHFR gene, C677T and A1298C, are associated with reduced enzyme activity and may be associated with breast cancer susceptibility. (medsci.org)
Enzyme10
The term may refer to a polymorphism itself, as detected through the differing locations of restriction enzyme sites, or to a related laboratory technique by which such differences can be illustrated. (wikipedia.org)
The basic technique for the detection of RFLPs involves fragmenting a sample of DNA with the application of a restriction enzyme, which can selectively cleave a DNA molecule wherever a short, specific sequence is recognized in a process known as a restriction digest. (wikipedia.org)
In allele A, the genome is cleaved by a restriction enzyme at three nearby sites (triangles), but only the rightmost fragment will be detected by the probe. (wikipedia.org)
In the third schematic, the probe and restriction enzyme are chosen to detect a region of the genome that includes a variable number tandem repeat (VNTR) segment (boxes in schematic diagram). (wikipedia.org)
The basic technique of identifying such restriction fragment length polymorphisms involve fragmenting a sample of DNA by a restriction enzyme, which can recognize and cut DNA wherever a specific short sequence occurs, in a process known as a restriction digest. (microbenotes.com)
If two organisms differ in the distance between sites of cleavage of a particular restriction endonuclease, the length of the fragments produced will differ when the DNA is digested with a restriction enzyme. (microbenotes.com)
The PCR products of 875bp (described above) of Pfdhps gene were digested with FokI enzyme (New England Biolabs, Ipswich, MA, USA) as per manufacture instruction's which generated 538bp, 315bp and 22bp fragments whenever mutant allele was present, and two fragments of 853bp and 22bp were seen for wild type allele. (cdc.gov)
PCR amplification of this region showed DNA bands of different size and number that could be further differentiated by using restriction enzyme AluI [ 10 ]. (hindawi.com)
Non-coding regions of DNA containing a restriction site for the enzyme Alu 1. (ojp.gov)
DNA first undergoes restriction enzyme digestion, and a subset of DNA fragments is then selected for PCR amplification and visualization. (ojp.gov)
RFLPs1
Coagulase gene PCR-RFLPs exhibited 10 patterns that ranged from 1 to 8 fragments with AluI digestion. (hindawi.com)
Microsatellite1
Neuronal nitric oxide synthase gene DNA polymorphism CA repeat Microsatellite Population study Japanese Human Heredity Short Communication Hum Hered 1997;47:58-59 Received. (karger.com)
Haplotypes1
Here we present a rapid and reliable method to distinguish rabbits that differ at their MHC class I and class II loci, and present 34 class I and 16 class II haplotypes determined by restriction frag-ment-length polymorphism analysis of outbred heterozygous rabbits. (wustl.edu)
C677T2
Conclusions: We conclude that MTHFR gene C677T polymorphism has no role in predisposition to the migraine in our population and cannot serve as a predictive factor for the risk of migraine. (scirp.org)
Furthermore, it has been shown in patients with breast cancer a risk of presenting with an aggressive biophenotype about twice or three times higher in the presence of the C677T and A1298C polymorphisms, respectively. (medsci.org)
Molecular1
A cloned, 500 base pair (bp) restriction fragment from the chloroplast genome of Pinus contorta hybridized to restriction fragments of a previously known polymorphism when used in molecular mapping experiments with total cellular DNAs of P. banksiana and P. contorta. (researchgate.net)
Detect1
A number of markers are now available to detect nuclear DNA polymorphisms. (intechopen.com)
Digestion1
Once the the DNA is isolated from the sample it is subjected to restriction digestion using restriction enzymes. (microbenotes.com)
MTHFR1
No significant differences were found in the genotypes of the two polymorphisms of the MTHFR gene between cases and controls. (medsci.org)
Loci1
It is also used in identification of recombination rate in the loci between restriction sites. (wikipedia.org)
Mutation1
In allele a, restriction site 2 has been lost by a mutation, so the probe now detects the larger fused fragment running from sites 1 to 3. (wikipedia.org)
Allele3
The second diagram shows how this fragment size variation would look on a Southern blot, and how each allele (two per individual) might be inherited in members of a family. (wikipedia.org)
In allele c, there are five repeats in the VNTR, and the probe detects a longer fragment between the two restriction sites. (wikipedia.org)
In allele d, there are only two repeats in the VNTR, so the probe detects a shorter fragment between the same two restriction sites. (wikipedia.org)
Analysis1
Of 20 retrievable isolates from 27 XDR-TB index cases, restriction fragment length polymorphism analysis showed clustering among 13 (65%), with 11 (55%) due to recent transmission by n-1 method and an identifiable household source in only three (27.2%) of the 11 cases. (ersjournals.com)
Relevance1
Functional relevance of yet another GSTO2 polymorphism, identified at the 5' untranslated (5'UTR) gene region (GSTO2*A183G, rs2297235), has not been clearly discerned so far. (cancerindex.org)
Sequence3
A restriction fragment length polymorphism is said to occur when the length of a detected fragment varies between individuals, indicating non-identical sequence homologies. (wikipedia.org)
To sequence the f1 and f2 fragments of pfmdr1 gene, two new pairs of sequencing primers were used (Technical Appendix Table). (cdc.gov)
This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)
Linkage1
All of the genotypes analyzed were in Hardy-Weinberg equilibrium, but different degrees of linkage were observed for polymorphisms in the CYP1A1 and EPHX1 genes. (scielo.br)
Population2
Cholesteryl ester transfer protein (CETP) DNA polymorphisms Population study Italians Greeks Original Paper Hum. (karger.com)
Our findings showed that C588T polymorphism of GABRG2 is a risk variant for IGE in Pakistani population. (hindawi.com)
Patients2
For this purpose, we genotyped exon2, exon5 ( C540T and C588T ), exon7 ( T813C ), exon8 ( K289M ), and exon9 of GABRG2 gene by restriction fragment length polymorphism and Sanger's sequencing in 87 drug-responsive idiopathic generalized epilepsy patients, 55 drug-resistant epilepsy patients, and 83 healthy controls. (hindawi.com)
Pour identifier les espèces de Leishmania , des techniques moléculaires ont été appliquées sur des échantillons prélevés chez 64 patients atteints de leishmaniose cutanée orientés vers l'hôpital régional d'Herat en 2013. (who.int)
Differences1
Many DNA fragments with slight differences in length are produced. (microbenotes.com)
Risk1
Concomitance of Polymorphisms in Glutathione Transferase Omega Genes Is Associated with Risk of Clear Cell Renal Cell Carcinoma. (cancerindex.org)
Confirm1
To confirm this polymorphism, the PCR product was purified using ExoSAP-IT followed by sequencing by Applied Biosystems 3130XL Automated Sequencer using the ABI BigDye Ver 3.1. (researchgate.net)
Method1
A highly sensitive method for detecting polymorphisms in DNA. (ojp.gov)
Results1
Our results show that one out of 60 bulls tested exhibited polymorphism (G→T) at position 559 in exon 4 of SLC35A3 gene. (researchgate.net)
Study2
The current study aimed to use Coagulase gene polymorphism to identify methicillin-resistant Staphylococcus aureus (MRSA) subtypes isolated from nasal carriers in Minia governorate, Egypt, evaluate the efficiency of these methods in discriminating variable strains, and compare these subtypes with antibiotypes. (hindawi.com)
The aim of this study was to use coa gene polymorphism to identify MRSA subtypes isolated from nasal carriers in Minia governorate, Upper Egypt, evaluate the efficiency of these typing methods in discriminating variable strains, and compare these subtypes with antibiotypes. (hindawi.com)