• Using mouse models, we discovered that mutations in murine Col4a1 and Col4a2 genes affected the balance between lung epithelial progenitors and differentiated cells. (biomedcentral.com)
  • The multifunctional regulator nuclear factor erythroid 2-related factor (Nrf2) is considered not only as a cytoprotective factor regulating the expression of genes coding for anti-oxidant, anti-inflammatory and detoxifying proteins, but it is also a powerful modulator of species longevity. (springer.com)
  • The major characteristics of Nrf2 are to some extent mimicked by Nrf2-dependent genes and their proteins including heme oxygenase-1 (HO-1), which besides removing toxic heme, produces biliverdin, iron ions and carbon monoxide. (springer.com)
  • The ~1.5 Mb candidate region was shown to contain five protein-coding genes. (biomedcentral.com)
  • We also identified several genes that do not appear to be protein coding, and generated 17 kb of novel transcript sequence data from re-sequencing 97 EST clones. (biomedcentral.com)
  • The candidate interval was shown to exhibit an extraordinary level of alternate splicing - 19 transcripts were identified for the 5 protein coding genes, but it appears that a significant portion (14/19) of these alternate transcripts did not have an open reading frame, hence their functional role is questionable. (biomedcentral.com)
  • We therefore analyse the role of imprinted genes in multiple tissues in two affected generations of an established murine model of the developmental origins of health and disease using microarrays and quantitative RT-PCR. (prolekarniky.cz)
  • However, we propose that those imprinted genes that are affected may play important roles in the foetal response to undernutrition and potentially its long-term sequelae. (prolekarniky.cz)
  • at the 1986 ISIR-TNO Annual Meeting, September 7-12, 1986, reported the production of transgenic mice carrying mouse interferon beta or gamma genes coupled to the mouse MT-I or heat shock promoters. (justia.com)
  • Gordon and Ruddle, Science, 214:1244 (1981) claimed integration and stable germline transmission of genes injected into mouse pronuclei. (justia.com)
  • B)The use of BACs reduces the frequency with which specific genes will be cut within the coding region by restriction enzymes and divided up among two or more clones. (easynotecards.com)
  • Suboptimal in utero environments such as poor maternal nutrition and gestational diabetes can impact fetal birth weight and the metabolic health trajectory of the adult offspring. (bvsalud.org)
  • Analysis of beta 2-microglobulin gene expression in the developing mouse embryo and placenta. (ulaval.ca)
  • Staehelin suggested that an animal could be protected against viral infection by inserting a gene encoding the Mx protein into that animal, preferably at the single cell embryo stage. (justia.com)
  • gse97554] Primate fetal hepatic response to maternal obesity: epigenetic sig. (aging-research.group)
  • Similar current changes after PKA activation were obtained from human embryonic kidney 293t cells transfected with the wild-type heat transducer protein vanilloid receptor 1 (VR-1). (jneurosci.org)
  • The gene encodes the interferon of a different animal species and therefore its expression product is less toxic to the host at an embryonic, fetal, neonatal or junvenile stage of development. (justia.com)
  • Given that young adults born premature also demonstrate skeletal muscle dysfunction, future studies are merited to determine whether this dysfunction as well as reduced aerobic capacity is due to reduced mitochondrial oxidative capacity and metabolic dysfunction. (frontiersin.org)
  • Despite the mounting evidence of both skeletal muscle and glucose handling impairments after premature birth, the specific skeletal muscle metabolic alterations underlying these physiologic changes in preterm born adults have not been well-identified. (frontiersin.org)
  • This approach includes an emphasis on epigenetic causes of adult chronic non-communicable diseases. (wikipedia.org)
  • However, the detailed epigenetic processes involved in maturation from fetal to adult CMs and in cardiac disease leading to terminal heart failure have not been fully uncovered, yet. (nature.com)
  • Nor do we find any evidence that the epigenetic reprogramming of ICRs in the germline is susceptible to nutritional restriction. (prolekarniky.cz)
  • Heterocellular hereditary persistence of fetal hemoglobin (HPFH) is a common multifactorial trait characterized by a modest increase of fetal hemoglobin levels in adults. (biomedcentral.com)
  • These studies in turn led to greater interest in the roles of developmental plasticity and early life environmental exposures in adult disease. (wikipedia.org)
  • Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
  • end point is a decrease in fetal/pup body weights observed after oral, dermal, and inhalation exposures of dams to NMP. (cdc.gov)
  • Oral, 90- and decrements in fetal/pup body weights following exposures to day exposures of rats and mice to NMP result in decrements in NMP vapor. (cdc.gov)
  • AND-34/BCAR3 (Breast Cancer Anti-Estrogen Resistance 3) associates with the focal adhesion adaptor protein, p130CAS/BCAR1. (molvis.org)
  • Keap1 is a cysteine-rich protein, known to be anchored to actin cytoskeleton [ 5 ], serving as an adaptor protein for the Cul3-dependent E3 ubiquitin ligase complex. (springer.com)
  • In adipose tissue, insulin stimulates glucose uptake by mediating the translocation of GLUT4 from intracellular vesicles to the plasma membrane. (bvsalud.org)
  • A new technique has been presented that results in an acceleration of delayed primary healing with full thickness skin and subcutaneous tissue coverage. (who.int)
  • The intestinal epithelium incorporates several distinct cell populations, including the rapidly dividing intestinal stem cells (ISCs) that facilitate the typical four-to-five day turnover cycle of the adult intestinal epithelium. (stemcell.com)
  • We assessed whether cell cycle inhibitors that restrict stem cell populations in other tissues may participate in limiting neural stem cell reactivity in vivo. (rupress.org)
  • While AND-34 is widely expressed in wild type mice, histologic analysis of multiple organs in AND-34 −/− mice is unremarkable and analyses of lymphocyte development show no overt changes. (molvis.org)
  • The focus of Dr. Berg's work is on the signaling proteins and pathways regulating T lymphocyte development, differentiation, activation, and migration, with a strong emphasis on T-cell receptor (TCR) signaling. (cuanschutz.edu)
  • For example, prostaglandins activate G s -protein-coupled receptors and initiate cAMP- and protein kinase A (PKA)-mediated processes. (jneurosci.org)
  • The translocation and the current potentiation were abolished in the presence of an A-kinase anchoring protein (AKAP) inhibitor. (jneurosci.org)
  • Here, we review how super-resolution microscopy approaches have allowed investigation of the characteristics of plasma membrane-fused GLUT4 and further discuss regulatory step(s) involved in the GLUT4 dispersal machinery, introducing the scaffold protein EFR3 which facilitates localisation of phosphatidylinositol 4-kinase type IIIα (PI4KIIIα) to the cell surface. (bvsalud.org)
  • Her work on the T cell tyrosine kinase ITK has revealed a critical role for this TCR signaling protein in autoreactive T cell trafficking into tissues such as the pancreas and the intestine. (cuanschutz.edu)
  • The cyclin-dependent kinase inhibitor, p21 cip1/waf1 (p21), maintains hematopoietic stem cell quiescence, and we evaluated its role in the regenerative response of neural tissue after ischemic injury using the mice deficient in p21. (rupress.org)
  • Previously, we found that Lactobacillus rhamnosus CNCM I-3690 can protect intestinal barrier functions in mice inflammation model. (nature.com)
  • Maternal obesity alters placental tissue function and morphology with a corresponding increase in local inflammation. (aging-research.group)
  • Vaccination may be with attenuated or inactivated virus, or with viral proteins produced by genetic engineering techniques. (justia.com)
  • In contrast to previous findings in heart tissue, expression of the pathological gene program in heart failure was not accompanied by changes in the CM DNA methylome but by active histone marks. (nature.com)
  • In this work, we have studied the feasibility of providing tissue-specific selectivity to replication-competent adenoviruses through the regulation of the late structural protein fiber (L5 gene). (oncotarget.com)
  • Molecular cloning and transcriptional mapping of the mouse cellular retinoic acid-binding protein gene. (ulaval.ca)
  • Tissue-specific activity of the pro-opiomelanocortin (POMC) gene and repression by glucocorticoids. (ulaval.ca)
  • A transgenic mouse with enhanced viral resistance transmissible to its offspring is prepared by introduction of a gene encoding a human interferon having antiviral activity into a host mouse. (justia.com)
  • Preferably, the human beta interferon gene is introduced into the cells of a mouse. (justia.com)
  • Apparently, Kawade had not determined whether the chimeric gene was actually expressed in the mice, but had already been able to express it in cultured cells induced by Cd.sup.2+ or by heat. (justia.com)
  • He taught that the gene of choice would be the one encoding the Mx protein that is normally found in the species of animal to be protected. (justia.com)
  • Histone modifications and chromatin-associated protein complexes are crucially involved in the control of gene expression, supervising cell fate decisions and differentiation. (researchgate.net)
  • This led to the fetal origins hypothesis of the origins of adult diseases, which proposed that this relationship was caused by differences in early life nutrition, with a supporting theory that birthweight is connected to the development of chronic disease. (wikipedia.org)
  • The World Congress on Fetal Origins of Adult Disease held two meetings - one in 2001 and the other in 2003 - summarizing then-new research in these areas. (wikipedia.org)
  • However, reshaping of the epigenome of these terminally differentiated cells during fetal development, postnatal maturation, and in disease remains unknown. (nature.com)
  • Dexamethasone phosphate/DEMO is indicated in the treatment of coronavirus disease 2019 (COVID-19) in adult and adolescent patients (aged 12 years and older with body weight at least 40 kg) who require supplemental oxygen therapy. (who.int)
  • Intestinal organoids are three-dimensional (3D) in vitro tissue cultures that model the in vivo intestine. (stemcell.com)
  • Neural precursor cells from adults have exceptional proliferative and differentiative capability in vitro yet respond minimally to in vivo brain injury due to constraining mechanisms that are poorly defined. (rupress.org)
  • This hypothesis suggested that poor growth during the fetal and infant stages can cause a development of type 2 diabetes later in life. (wikipedia.org)
  • The intestinal niche has been well-characterized, and shown to consist of spatial gradients of high WNT and Epithelial Growth Factor (EGF), while Bone Morphogenetic Protein (BMP) signals are inhibited. (stemcell.com)
  • gse43685] Early growth response protein-1 coordinates lipotoxicity-associate. (aging-research.group)
  • it is reduced in fetal growth restriction and increased in fetal overgrowth. (bvsalud.org)
  • gse39445] Effect of sleep restriction on the human transcriptome during exte. (aging-research.group)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • If you ever had a chance to see a heart working in real time, you might have noticed the thick fatty tissue that surrounds it. (sott.net)
  • Since the introduction of the mouse small intestinal organoid model in 2009, 1 there has been an avalanche of developments in this field, including development of culture conditions for human organoids derived from primary colonic tissue, 2 as well as from human pluripotent stem cells (hPSCs). (stemcell.com)
  • 17 This property of rapid regeneration at intestinal stasis makes the intestine a uniquely convenient model system for epithelial cell biology and adult stem cell biology studies both inside and outside the specific context of intestinal function. (stemcell.com)
  • The therapeutic effect of umbilical cord-derived mesenchymal stem cells(hUC-MSCs) in combination with pirfenidone(PFD) on pulmonary fibrosis in mice and its possible mechanism were investigated. (researchsquare.com)
  • monitor for adverse reactions to concomitant highly protein-bound drugs and narrow therapeutic index drugs (e.g., warfarin, cyclosporine, amphotericin B) when initiating or increasing the dosage of dronabinol capsules. (nih.gov)
  • The adult intestinal epithelium is primarily composed of six cell types that are arranged in a crypt-villus structure (Figure 1). (stemcell.com)
  • Linkage analysis by restriction fragment length polymorphism (RFLP) in multiple family members can be used. (medscape.com)
  • These results demonstrate the loss of AND-34 dysregulates focal adhesion complex signaling in lens epithelial cells and suggest that AND-34-mediated signaling is required for maintenance of the structural integrity of the adult ocular lens. (molvis.org)
  • The 26S subgenomic mRNA is translated to produce the polyprotein from which E1, E2, and C structural proteins are processed. (cdc.gov)
  • Based on this method 13 , 14 , we used a nuclear staining strategy to isolate CM nuclei from intact prenatal and postnatal human heart tissue and subjected these nuclei to comprehensive analysis of the epigenome during prenatal development, postnatal maturation, and in heart failure. (nature.com)
  • Here we describe the human CM epigenome during prenatal development and postnatal maturation of the heart from infant to adult age and in terminal failure. (nature.com)
  • Healthy human adults were recruited to a sleep lab at Washington State University and remained there 7 consecutive days. (aging-research.group)
  • Our body organs and tissues work much better when they use ketones as a source of fuel, including the brain, heart and the core of our kidneys. (sott.net)
  • Lung tissues were collected on day 21 to detect pathological changes and the expression of collagen content and regulator of G protein signaling 2(RGS2). (researchsquare.com)
  • Lung development timeline and type IV collagen expression in the chicken and the mouse. (biomedcentral.com)
  • We previously reported that when metabolically challenged by a high-fat diet, placental mTORKO (mTORKOpl) adult female offspring develop obesity and insulin resistance, whereas placental TSC2KO (TSC2KOpl) female offspring are protected from diet-induced obesity and maintain proper glucose homeostasis. (bvsalud.org)
  • Although steady-state conditions revealed no increase in primitive cell proliferation in p21-null mice, a significantly larger fraction of quiescent neural precursors was activated in the hippocampus and subventricular zone after brain ischemia. (rupress.org)
  • Therefore, p21 is an intrinsic suppressor to neural regeneration after brain injury and may serve as a common molecular regulator restricting proliferation among stem cell pools from distinct tissue types. (rupress.org)
  • Nuclear positioning, germinal vesicle breakdown, spindle migration, spindle rotation, chromosome segregation, and polar body extrusion are the most critical cellular processes during oocyte meiosis I and II, and a growing number of studies primarily using the mouse oocyte model revealed that actin filaments were critical for these processes, especially for spindle migration. (bioone.org)
  • Oxidative stress generated during such stressful conditions may damage DNA and proteins, and as a consequence the cellular processes are disturbed. (springer.com)
  • This study demonstrates detrimental mitochondrial and muscular outcomes in the adult male rat exposed to postnatal hyperoxia. (frontiersin.org)
  • Following initial vacuolization and liquefaction of the lens cortex first observed at postnatal day three, posterior lens rupture occurs in all AND-34 −/− mice, beginning as early as three weeks and seen in all mice at three months. (molvis.org)
  • CBM is used in many domains including clinical trials management, ICR, in vivo imaging, and tissue banks and pathology tools. (nih.gov)
  • Obesity, a prevalent condition in adults and children, impairs bone marrow (BM) function. (aging-research.group)
  • lion adults are obese and 2.1 bil ion attributed to obesity. (who.int)
  • Apart from exciting nociceptors, inflammatory mediators increase nociceptor responsiveness, frequently by initiating G-protein-mediated processes, to cause hyperalgesia. (jneurosci.org)
  • Owing to technical restrictions, these studies were performed in heart tissue and therefore the affected cell type(s) could not be identified. (nature.com)
  • Purification of CM nuclei by fluorescence-assisted sorting has led to the identification of cell-type-specific mCpG and histone modification signatures in CMs during mouse heart development and maturation 11 . (nature.com)
  • Patients entered the model insured under Medicaid and were treated under state-specific restrictions by Metavir fibrosis stage (base case) or all treated (all-patient strategy) with an approved all-oral regimen (ledipasvir/sofosbuvir [LDV/SOF] for 8 weeks or 12 weeks, depending on cirrhosis status, viral load, and state-specific LDV/SOF restrictions). (cdc.gov)
  • Untreated patients were assumed to age into Medicare at 65 years, where they were treated with LDV/SOF without restriction by fibrotic stage. (cdc.gov)
  • RESULTS: The sustained virologic response (SVR) rate of the current Medicaid LDV/SOF restriction strategy was 75.2% versus 95.9% if all LDV/SOF-eligible patients were treated under Medicaid. (cdc.gov)
  • An example of this type of response would be when oxygen is deprivation which causes a change in blood flow that would help with the expense of less critical tissues. (wikipedia.org)
  • Lastly, we revealed that addition of type IV collagen protein induced myofibroblast proliferation and migration in monolayer culture and increased the formation of mesenchymal-epithelial septal-like structures in co-culture. (biomedcentral.com)
  • To improve the safety of oncolytic adenoviruses a control of the viral tropism based on tissue-specific miRNA regulation has been exploited. (oncotarget.com)
  • Maternal 5-HT1A-receptor (R) is required for the timely development of the hippocampus and the establishment of emotional behaviors in Swiss-Webster (SW) mice. (aging-research.group)
  • A Stereo Investigator system provides you with the means to make the most accurate, unbiased quantification of the number, length, area, and volume of cells, subcellular and macro structures in your tissue specimens. (mbfbioscience.com)
  • Western blot analysis and in situ hybridization confirmed the presence of AND-34 RNA and protein in lens epithelial cells, particularly at the lens equator. (molvis.org)
  • Flow cytometry analysis of CD19 was performed by staining Raji CD19 Knock out cells with 0.25 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-82, yellow histogram) or 0.25 µg CD19 Monoclonal Antibody (HIB19), PE, eBioscience™ (Product # 12-0199-42, blue histogram). (thermofisher.com)
  • Tissue-specific detargeting by miRNAs has been demonstrated to be a potent strategy to restrict adenoviral replication to cancer cells. (oncotarget.com)
  • Noticeably, mice treated with Ad-L5-8miR148aT showed an attenuation of adenoviral-induced hepatotoxicity but retained full lytic activity in cancer cells and exhibited robust antitumoral responses in patient-derived xenografts. (oncotarget.com)
  • February 12, 1987) observed inhibition of influenza virus in NIH 3T3 mouse cells genetically modified to express the mouse protein Mx. (justia.com)
  • We consider how dispersal may be linked to the control of transporter activity, consider whether macro-organisation may be a widely used phenomenon to control proteins within the plasma membrane, and speculate on the origin of different forms of GLUT4-vesicle exocytosis. (bvsalud.org)