The use of signal molecules rather than drugs to encourage tissue regeneration is likely to increase in popularity in the coming years as the process becomes increasingly refined. (transhumanist-party.org)
The levels of catalase (CAT) and glutathione (GSH) were measured in PC12 cells and Drosophila brain tissue. (sdbonline.org)
GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
Stem cell transplantation is a promising strategy for therapeutic cardiac regeneration, but current therapies are limited by inefficient interaction between potentially beneficial cells (either exogenously transplanted or endogenously recruited) and the injured tissue. (regenerativemedicine.net)
Here we apply targeted nanomedicine to achieve in vivo cell-mediated tissue repair, imaging and localized enrichment without cellular transplantation. (regenerativemedicine.net)
Rejuvenation therapies aim to reverse or repair age-related cellular changes such as molecular waste, calcification , tissue stiffening , loss of stem cell function , genetic alterations, and impaired energy production . (fightaging.org)
The research group has already worked for several years to develop electrodes that are thin and flexible enough not to disturb the brain tissue, and with material that does not irritate the cells nearby. (regenerativemedicine.net)
In most mammals, new neurons are born throughout adulthood in two regions of the brain: The subgranular zone (SGZ), part of the dentate gyrus of the hippocampus, where neural stem cells give birth to granule cells (implicated in memory formation and learning). (wikipedia.org)
Neural stem cells (NSCs) are the self-renewing, multipotent cells that generate the main phenotypes of the nervous system. (wikipedia.org)
In the field of stem cells, cumulative evidence has shown that mitochondrial transfer contributes to stem cell-triggered repair of damaged cells [ 9 ]. (biomedcentral.com)
A systematic survey of the scientific literature shows that stem cell therapy can have a statistically significant impact on animal models of spinal cord injury, and points the way for future studies. (regenerativemedicine.net)
Stem cell therapy aims to use special regenerative cells (stem cells) to repopulate areas of damage that result from spinal cord injuries, with the hope of improving the ability to move ("motor outcomes") and to feel ("sensory outcomes") beyond the site of the injury. (regenerativemedicine.net)
These limitations hamper each individual study's statistical power to detect the true effects of the stem cell implantation. (regenerativemedicine.net)
In this case the authors assessed 156 published studies that examined the effects of stem cell treatment for experimental spinal injury in a total of about 6000 animals. (regenerativemedicine.net)
The authors went on to use their analysis to explore the effects of bias (whether the experimenters knew which animals were treated and which untreated), the way that the stem cells were cultured, the way that the spinal injury was generated, and the way that outcomes were measured. (regenerativemedicine.net)
Dentate gyrus3
The experiments advocating for the presence of adult neurogenesis have focused on how dual antigen retrieval finds that DCX antibodies are staining many cells within the adult human dentate gyrus. (wikipedia.org)
the dentate gyrus cells stained with DCX have been shown to have a mature morphology, contrasting the idea that novel neurons are being generated within the adult brain. (wikipedia.org)
JWX restored the expression of BDNF in the hippocampal dentate gyrus reduced by CORT-exposure. (bvsalud.org)
Proteins4
Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
These compounds generally penetrate cell membranes, act on specific target proteins in cells, regulate intracellular signaling pathways, and cause some changes in cell phenotype. (chemscene.com)
We decellularized the metastatic lung sections, measured the micromechanics of the tumor ECM, and stained the sections for ECM proteins, proliferation, and cell death markers. (ibecbarcelona.eu)
Cellular identity is determined by the transcriptional profile which comprises the subset of mRNAs, and therefore proteins, being expressed by a cell at a given point in time. (silverchair.com)
Intracellular4
Time-lapse videography of living cells captures spectacular intracellular mitochondrial movement, and this movement promotes mitochondrial connections to form a dynamic mitochondrial network [ 3 ]. (biomedcentral.com)
In addition to intracellular mitochondrial movement, intercellular mitochondrial transfer between mammalian cells has recently been discovered. (biomedcentral.com)
Intercellular mitochondrial transfer can be regarded as an extension of intracellular mitochondrial movement or intercellular communication, which undoubtedly increases the mtDNA content of the recipient cells and restores the respiration and survival of the recipient cells. (biomedcentral.com)
Increasingly, complimentary cell culture techniques, which recapitulate the developmental microenvironment, are employed to coax cells to adopt new identities by indirectly regulating transcription factor activity via intracellular signalling pathways. (silverchair.com)
Differentiation3
Epigenetic Regulation during Primordial Germ Cell Development and Differentiation. (ericmiskalab.org)
The dynamics of RACK1 levels in isolated adult SC of mice, i.e., progressively high during differentiation and low compared to proliferating conditions, and RACK1 silencing indicated that RACK1 promotes both the formation of myotubes and the accretion of nascent myotubes. (sdbonline.org)
Here, we explore the evolution of reprogramming and directed differentiation approaches within the context of hepatocyte to β-cell transdifferentiation focussing on how the introduction of new techniques has improved our ability to generate β-cells. (silverchair.com)
Based on these findings, the researchers proposed the bold idea of directly transplanting entire mitochondria, rather than drugs or whole cells, into lesions to explore the rescue effects of exogenous mitochondria. (biomedcentral.com)
Hippocampal1
some studies have reported that in adult humans about 700 new neurons are added in the hippocampus every day, while more recent studies show that adult hippocampal neurogenesis does not exist in humans, or, if it does, it is at undetectable levels. (wikipedia.org)
Involves1
Osteoinduction involves recruiting immature cells and stimulating them to change into preosteoblasts. (transhumanist-party.org)
Therapeutic4
In addition, we also discuss the existing methods of artificial mitochondrial transfer/transplantation (AMT/T) and their therapeutic effects on diseases to explore the potential applications of mitochondrial transfer and transplantation in the treatment of mitochondrial-related diseases, mitochondrial diseases, and cancer. (biomedcentral.com)
Iron nanoparticles are conjugated with two types of antibodies (one against antigens on therapeutic cells and the other directed at injured cells) to produce magnetic bifunctional cell engager (MagBICE). (regenerativemedicine.net)
The antibodies link the therapeutic cells to the injured cells, whereas the iron core of MagBICE enables physical enrichment and imaging. (regenerativemedicine.net)
A better understanding of the levers governing transcription factor activity benefits our ability to generate therapeutic cell types at will. (silverchair.com)
Apoptosis1
MATERIALS AND METHODS: IE-6 and Caco-2 cells were used to evaluate the effect of Lico A on apoptosis, proliferation, and migration of IECs. (bvsalud.org)
Regulate2
RNA uridyl transferases TUT4/7 differentially regulate miRNA variants depending on the cancer cell type. (ericmiskalab.org)
dGLYAT was found to regulate Gadd45 -mediated JNK pathway activation and cell invasion. (sdbonline.org)
Development2
Though avians have been used primarily to study early embryonic development, in recent decades the developing chick has played a critical role in the examination of neurogenesis and regeneration as the young chick is capable of neuronal-turnover at a young age, but loses the neurogenerative capacity into adulthood. (wikipedia.org)
The identification of key master regulator transcription factors (which distinguish one body part from another) during embryonic development has been central in developing transdifferentiation protocols. (silverchair.com)
Cell damage was assessed using MTT assays, and the lactate dehydrogenase (LDH) release rate and flow cytometry were used to detect the accumulation of reactive oxygen species (ROS) and mitochondrial membrane potential respectively. (sdbonline.org)
In Drosophila with depleted RACK1 in all muscle cells or, specifically, in SC lineage resulted in a delayed recovery of skeletal muscle after physical damage as well as the low presence of active SC in the wound area. (sdbonline.org)
Cellular3
Multiple research groups have developed artificial mitochondrial transfer/transplantation (AMT/T) methods that transfer healthy mitochondria into damaged cells and recover cellular function. (biomedcentral.com)
Transdifferentiation is a type of cellular reprogramming involving the conversion of one differentiated cell type to another. (silverchair.com)
Here, we discuss how transcription factors, and their unique position as the gatekeepers of cellular identity, are exploited in cell reprogramming protocols by exploring work focusing on one reprogramming paradigm - the transdifferentiation of hepatocytes to pancreatic beta cells (β-cell). (silverchair.com)
Potentially1
This cell type conversion potentially represents a novel therapy in T1D treatment. (silverchair.com)
Biology1
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of The Digital Cell: Cell Biology as a Data Science, available on its website in hardcover format. (cshlpress.com)
Genetic1
We consider how, over the last 20 years, advances in genetic engineering and cell culture techniques have improved the efficiency and efficacy of the transdifferentiation process and brought us closer to a clinically relevant therapy for type 1 diabetes (T1D). (silverchair.com)
Study4
Planarian are one of the earliest model organisms used to study regeneration with Pallas as the forefather of planarian studies. (wikipedia.org)
Collectively, this study provides the first evidence that transient levels of the evolutionarily conserved factor RACK1 are critical for adult SC activation and proper skeletal muscle regeneration, favoring the efficient progression of SC from a committed to a fully differentiated state. (sdbonline.org)
This study aims to understand the function and mechanism of Drosophila Glycine N-acyltransferase (GLYAT) in cell invasion. (sdbonline.org)
This study presents an in-silico model replicating a microfluidic assay where endothelial cells sprout into a biomimetic extracellular matrix, specifically, a large-pore, low-concentration fibrin-based porous hydrogel, influenced by chemotactic factors. (ibecbarcelona.eu)
Researchers2
At Lund University in Sweden, researchers have, for the first time, succeeded in implanting an ultrathin nanowire-based electrode and capturing signals from the nerve cells in the brain of a laboratory animal. (regenerativemedicine.net)
The researchers want to try and reduce the size of the base to which the nanowires are attached, improve the connection between the electrode and the electronics that receive the signals from the nerve cells, and experiment with the surface structure of the electrodes to see what produces the best signals without damaging the brain cells. (regenerativemedicine.net)
Experiments1
Such thin electrodes have previously only been used in experiments with cell cultures. (regenerativemedicine.net)
Early1
RACK1 was expressed transiently in the skeletal muscle of post-natal mice, being abundant in the early phase of muscle growth and almost disappearing in adult mature fibers. (sdbonline.org)
Emerging evidence suggests that neural microvascular pericytes, under instruction from resident glial cells, are reprogrammed into interneurons and enrich local neuronal microcircuits. (wikipedia.org)
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species. (lookformedical.com)
Loss2
Firstly, loss of dGLYAT suppressed scrib depletion- or Egr overexpression-induced JNK pathway activation and invasive cell migration. (sdbonline.org)
In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1). (lookformedical.com)
Increase1
For sensory outcomes the degree of improvement tended to increase with the number of cells introduced -- scientists are often reassured by this sort of "dose response," as it suggests a real underlying biologically plausible effect. (regenerativemedicine.net)
Model1
The axolotl is less commonly used than other vertebrates, but is still a classical model for examining regeneration and neurogenesis. (wikipedia.org)
Role1
Adult neurogenesis in rodents is reported to play a role in learning and memory, emotion, stress, depression, response to injury, and other conditions. (wikipedia.org)
Cancer1
Cell invasion is a crucial step of tumor metastasis , finding new regulators of which offers potential drug targets for cancer therapy. (sdbonline.org)
Memory1
Cold Spring Harbor Laboratory Press (CSHLP) announced the release of T-Cell Memory, available on its website in hardcover and eBook formats. (cshlpress.com)
Place1
We are pleased that we have succeeded in developing a functioning nano-electrode, getting it into place, and capturing signals from nerve cells, says Prof. Jens Schouenborg. (regenerativemedicine.net)
Found1
It was found that GSOs reversed GLU-induced reductions in cell viability and the LDH release rate, and rescued ROS accumulation. (sdbonline.org)
Specifically1
In photosynthetic organisms the rotor complex of the ATP synthase (blue and cyan) is specifically adapted to physiological needs of the plant or cyanobacterial cell. (silverchair.com)
Addition1
The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. (lookformedical.com)
Energy1
Mitochondria are essential organelles that not only serve as energy factories, but also have functions extending to cell signalling. (biomedcentral.com)