Responses voltage sensitive dye imaging. Medical search. Frequent questions

Voltage-sensitive dyes, also known as potentiometric dyes, are dyes which change their spectral properties in response to voltage changes. (wikipedia.org)
Potentiometric dyes are used to monitor the electrical activity inside cell organelles where it is not possible to insert an electrode, such as the mitochondria and dendritic spine. (wikipedia.org)
Grinvald A, Salzberg BM, Lev-Ram V, Hildesheim R (1987) Optical recording of synaptic potentials from processes of single neurons using intracellular potentiometric dyes. (yale.edu)

Many physiological processes are accompanied by changes in cell membrane potential which can be detected with voltage sensitive dyes. (wikipedia.org)
Fast-response probes: These are amphiphilic membrane staining dyes which usually have a pair of hydrocarbon chains acting as membrane anchors and a hydrophilic group which aligns the chromophore perpendicular to the membrane/aqueous interface. (wikipedia.org)
The chromophore is believed to undergo a large electronic charge shift as a result of excitation from the ground to the excited state and this underlies the putative electrochromic mechanism for the sensitivity of these dyes to membrane potential. (wikipedia.org)
A change in the voltage across the membrane will therefore cause a spectral shift resulting from a direct interaction between the field and the ground and excited state dipole moments. (wikipedia.org)
MDC researchers have now discovered that the channels are also sensitive to changes in membrane voltage. (phys.org)
Ehrenberg B, Farkas DL, Fluhler EN, Lojewska Z, Loew LM (1987) Membrane potential induced by external electric field pulses can be followed with a potentiometric dye. (yale.edu)
Fromherz P, Lambacher A (1991) Spectra of voltage-sensitive fluorescence of styryl-dye in neuron membrane. (yale.edu)
Gross D, Loew LM, Webb WW (1986) Optical imaging of cell membrane potential changes induced by applied electric fields. (yale.edu)
We engineered electrochromic fluorescence resonance energy transfer (eFRET) genetically encoded voltage indicators (GEVIs) with "positive-going" fluorescence response to membrane depolarization through rational manipulation of the native proton transport pathway in microbial rhodopsins. (janelia.org)
4) The article does not mention any alternative methods of estimating conduction velocity or latency such as membrane potential optical imaging (e.g. (scholarpedia.org)
Through photoactivation, the ChR2 channels pump cations into the cell, causing an increase of the voltage across the cell membrane (an action potential) and firing of the cell. (newport.com)
Voltage imaging has gradually become an important technique to reveal the impact of rapid changes in membrane voltage on cell physiology. (creative-bioarray.com)
Small molecule voltage imaging has a long history, and compounds with voltage-sensitive optical properties provide a shortcut for the fluorescence detection of membrane potential. (creative-bioarray.com)
Liu P, Miller E W. Electrophysiology, unplugged: imaging membrane potential with fluorescent indicators[J]. Accounts of chemical research, 2019, 53(1): 11-19. (creative-bioarray.com)
Miller E W. Small molecule fluorescent voltage indicators for studying membrane potential[J]. Current opinion in chemical biology, 2016, 33: 74-80. (creative-bioarray.com)

Strengths of imaging brain activity with voltage-sensitive dyes include the following abilities: Measurement of population signals from many areas may be taken simultaneously, and hundreds of neurons may be recorded from. (wikipedia.org)
We then used a genetically-encoded hybrid voltage sensor (hVOS) to image voltage from a specific population of neurons. (stanford.edu)
The introduction of voltage imaging to the CN creates the opportunity to investigate auditory processing circuitry in populations of neurons targeted on the basis of their genetic identity and their roles in sensory processing.Significance StatementThe cochlear nucleus uses dedicated circuitry to process and interpret information from the ear. (stanford.edu)
By targeting a genetically-encoded hybrid voltage sensor (hVOS) to identify neurons activated during the presentation of sound, the properties and function of these neurons become accessible to study in slices of mouse ventral cochlear nucleus. (stanford.edu)
These techniques allow us to see the voltage generated by our brain's firing neurons - telling us how networks of neurons develop, function and change over time. (edu.au)
Today, voltage imaging of cultured neurons is performed using dense arrays of electrodes onto which cells are grown (or cultured), or by applying light-emitting dyes that respond optically to changes in voltage on the surface of the cell. (edu.au)
Llinás RR, Sugimori M, Cherksey B (1989) Voltage-dependent calcium conductances in mammalian neurons. (yale.edu)
The resulting indicator, mNG-GECO1, is 60% brighter than GCaMP6s in vitro and provides comparable performance as demonstrated by imaging Ca dynamics in cultured cells, primary neurons, and in vivo in larval zebrafish. (janelia.org)
Using improved voltage-sensitive dye imaging technique we studied AP backpropagation in the basal dendrites of layer 5 pyramidal neurons of rat PFC. (uconn.edu)
The DA-mediated spatio-temporal pattern of dendritic calcium suppression is expected to occur during phasic dopaminergic signaling, when midbrain dopaminergic neurons generate a transient (0.5 s) burst of action potentials in response to a salient event in the animal's environment. (uconn.edu)
Using a microscope, laser light can be focused precisely on specific neurons containing light sensitive ion channels, or opsins, and causes those neurons to activate and signal each other. (newport.com)
Our results demonstrate that the amplitude of spontaneous miniature and evoked EPSCs in nTS neurons is significantly increased in Mecp2 -null mice and, accordingly, that mutant cells are more likely than wild- type cells to fire action potentials in response to primary afferent stimulation. (jneurosci.org)
Therefore, we hypothesized that nTS neurons in Mecp2 -null mice would exhibit exaggerated responses to primary afferent stimulation due to loss of this BDNF-dependent modulation. (jneurosci.org)
The present study demonstrates that nTS relay neurons in Mecp2 -null mice exhibit significantly larger EPSCs, and are more likely to fire action potentials in response to afferent stimulation, than in wild-type controls. (jneurosci.org)
Brown AM, Schwindt PC, Crill WE (1993) Voltage dependence and activation kinetics of pharmacologically defined components of the high-threshold calcium current in rat neocortical neurons. (yale.edu)

Fast" dyes are usually electrochromic dyes that can be used to monitor rapid changes in neuronal voltage. (creative-bioarray.com)

Contrast is a key visual variable in that it controls visibility and measurements from single-units to optical-imaging to fMRI find general consistency in that cortical visual areas respond in monotonically increasing functions to increases in contrast. (visionsciences.org)
Alonso BD, Lowe AS, Dear JP, Lee KC, Williams SC, Finnerty GT (2008) Sensory inputs from whisking movements modify cortical whisker maps visualized with functional magnetic resonance imaging. (springer.com)
While some differences between these studies could be partially explained by fundamental differences in the experimental approach (electrophysiology vs Ca 2+ imaging), the higher heterogeneity observed in supragranular layer with imaging approaches could point to specific transformations occurring between these cortical laminae (i.e. (jneurosci.org)
The model assumes that the geometry of V1 serves to organise multiple response properties into two cortical dimensions. (paulbourke.net)
Late evoked responses are generally used for studying higher cortical functions (eg, P300 in Alzheimer disease). (medscape.com)

At the Giardino lab, Yihe aims to leverage imaging techniques and slice electrophysiology to investigate the underlying mechanism of addiction and sleep disorders in BNST neuropeptide circuits. (stanford.edu)
A comparison of neuronal population dynamics measured with calcium imaging and electrophysiology. (janelia.org)
Our analysis highlights challenges in relating electrophysiology and imaging data, and suggests forward modeling as an effective way to understand differences between these data. (janelia.org)

Using whole-field illumination combined with intracellular application of voltage- or calcium-sensitive dyes, we found that AP propagation in two sister branches (branching off the same parent dendrite) may differ drastically in efficacy and high-frequency filtering properties. (uconn.edu)

This contact detection is conveyed by classes of mechanoreceptors whose neural activity is sensitive to either slow or fast time varying mechanical stresses acting at the base of the whiskers. (frontiersin.org)
Akkin T, Dave DP, Milner TE, Rylander HG (2004) Detection of neural activity using phase-sensitive optical low-coherence reflectometry. (springer.com)
Genetically encodable calcium ion (Ca) indicators (GECIs) based on green fluorescent proteins (GFP) are powerful tools for imaging of cell signaling and neural activity in model organisms. (janelia.org)

Her thesis work focused on interrogating hippocampal microcircuits with a hybrid genetically-encoded voltage indicator. (stanford.edu)

Measurements of brain activity using very similar protocols have been made using metrics ranging from single-unit responses to functional imaging in both animals and humans. (visionsciences.org)
To address this challenge, we developed the Swarm TM , a custom designed optical instrument for highly parallelized, multicolor measurements in excitable cells, simultaneously recording changes in voltage and calcium activities at high temporal resolution under optical stimulation. (frontiersin.org)

Developed by a team of physicists from the University of Melbourne and RMIT University, the device uses a diamond-based sensor that converts voltage signals at its surface directly into optical signals - this means we can see electrical activity as it happens. (edu.au)
Aguirre AD, Chen Y, Fujimoto JG, Ruvinskaya L, Devor A, Boas DA (2006) Depth-resolved imaging of functional activation in the rat cerebral cortex using optical coherence tomography. (springer.com)
Grinvald A, Frostig RD, Lieke E, Hildesheim R (1988) Optical imaging of neuronal activity. (yale.edu)
The most popular approach is to implant optical devices into the brains of freely moving animals, but the scattering and absorption of light in neural tissue requires an optical platform that provides enough power to excite the light-sensitive proteins, as well as the spatial resolution needed to stimulate specific regions of interest. (electrooptics.com)
Optogenetics allows neuroscientists to study neural processes by using an optical system to activate cell populations tagged with light-sensitive proteins. (electrooptics.com)
The compact design featuring high power LEDs, large numerical aperture optics, and fast photodiode detection enables all-optical individual well readout of 24-wells simultaneously from multi-well plates while maintaining sufficient temporal resolution to probe millisecond response dynamics. (frontiersin.org)
Voltage-sensitive optical dyes have revolutionized neuroscience research by depicting neuronal activity in real time and with high spatial resolution. (aip.org)
The lasers commonly employed in optical microscopy are high-intensity monochromatic light sources, which are useful as tools for a variety of techniques including optical trapping, lifetime imaging studies, photobleaching recovery, and total internal reflection fluorescence. (fsu.edu)

Using a voltage-sensitive dye we recorded glutamate receptor-independent signals arising predominantly from axons. (stanford.edu)

Methods: The molecular layer of the DG (dentate gyrus) of human epileptic tissue and rat nonepileptic tissue was electrically stimulated and the evoked responses were recorded with voltage-sensitive dye imaging to characterize the spatiotemporal properties. (pdffox.com)

In certain preparations the pharmacological effects of the dyes may be completely reversed by removing the staining pipette and allowing the neuron 1-2 hours for recovery. (wikipedia.org)

Slow-response probes: These exhibit potential-dependent changes in their transmembrane distribution which are accompanied by a fluorescence change. (wikipedia.org)
Typical slow-response probes include cationic carbocyanines and rhodamines, and ionic oxonols. (wikipedia.org)
Voltage sensitive dyes are supplied by many companies who offer fluorescent probes for biological applications. (wikipedia.org)
From commercial ion indicators to customized reactive fluorescent dye-labeled probes, our services can meet the diverse needs of customers and help you achieve satisfactory results with minimal cost. (creative-bioarray.com)

Finally, we discuss how multimodal imaging, along with valid physiological models, can ultimately be used to obtain quantitative estimates of physiological parameters in health and disease and provide an outlook for the future directions in neurovascular research. (springer.com)
We compute a quantitative image quality budget for this visual system and show how chromatic blurring dominates the visual acuity in these animals in shallow water. (rctn.org)

IWR-1-endo potently inhibits WNT signaling by blocking a cell-based WNT/β-catenin pathway reporter response with an IC₅₀ value of 180 nM (Chen et al. (stemcell.com)

Using loss-of-function pericyte-deficient mice, here we show that pericyte degeneration diminishes global and individual capillary CBF responses to neuronal stimuli, resulting in neurovascular uncoupling, reduced oxygen supply to the brain and metabolic stress. (nature.com)
Evoked potentials (EPs), or evoked responses, measure the electrophysiologic responses of the nervous system to a variety of stimuli. (medscape.com)

Staub C, De Schutter E, Knöpfel T (1994) Voltage-imaging and simulation of effects of voltage- and agonist-activated conductances on soma-dendritic voltage coupling in cerebellar Purkinje cells. (yale.edu)
Cummins TR, Xia Y, Haddad GG (1994) Functional properties of rat and human neocortical voltage-sensitive sodium currents. (yale.edu)

Colour film can actually be thought of as 3 layers of B+W film with one of the 3 different primary additive colour dyes in each layer. (naturephotographers.net)
The mappings described allow those properties of the visual field which can be predicted on the basis of spatial contiguity (texture, orientation, colour, contrast) to be available as response properties over the entire visual field. (paulbourke.net)

4 A voltage-sensitive MRI contrast agent could similarly transform neuroscience and neurology for humans by providing MRI-readable, functional brain information. (aip.org)

We tested two approaches based on fundamentally different voltage sensing technologies. (stanford.edu)
We further applied this general approach to GEVIs containing different voltage sensitive rhodopsin domains and various fluorescent dye and fluorescent protein reporters. (janelia.org)

In nonepileptic control DG from rats, CBZ also reduced the amplitude of the evoked response in the molecular layer of the DG as well as the spatial extent of the response. (pdffox.com)
Here, we used in vivo two-photon Ca 2+ imaging to probe the organization of the mouse primary auditory cortex and show that the spatial organization of neural response properties (frequency tuning) within the thalamorecipient layer (L3b/4) is more homogeneous than in supragranular layers (L2/3). (jneurosci.org)

To evaluate the Swarm screening system, we optimized a series of heterologous optogenetic spiking HEK293 cell assays for several voltage-gated sodium channel subtypes including Nav1.2, Nav1.5, and Nav1.7. (frontiersin.org)
We provide imaging applications based on calcium indicator, sodium indicator, potassium indicator, magnesium indicator, zinc indicator and other ionic indicators to meet the research needs of different customers. (creative-bioarray.com)

Using voltage-sensitive-dye imaging, we revealed a visuo-tactile convergent region that anatomically corresponds to the associative parietal cortex (APC). (hal.science)
However, they also found that a single-fibre solution is unable to provide enough light to stimulate light-sensitive biomolecules located several millimetres away from the cerebral cortex. (electrooptics.com)
The LGS model of the primary visual cortex suggests there is a direct relationship between globally represented objects (e.g. an oriented line) and various other response properties (e.g. orientation preference) which have a local geometry. (paulbourke.net)
Cepeda C, Radisavljevic Z, Peacock W, Levine MS, Buchwald NA (1992) Differential modulation by dopamine of responses evoked by excitatory amino acids in human cortex. (yale.edu)

Exposure to light chemically transforms the light sensitive grains into a latent image proportionate to the intensity of the light. (naturephotographers.net)
The two major concerns in safe laser operation are exposure to the beam and the electrical hazards associated with high voltages within the laser and its power supply. (microscopyu.com)

The core material for imaging brain activity with voltage-sensitive dyes are the dyes themselves. (wikipedia.org)
Figure 2: Impaired hemodynamic response in 1- to 2-month-old pericyte-deficient Pdgfrb +/− mice determined by IOS imaging (530 nm) at a time point when neuronal activity is unaffected as determined by LFP recordings. (nature.com)
Multi-electrode arrays, calcium imaging, voltage-sensitive dyes and intrinsic imaging measure mesoscale population activity. (visionsciences.org)
Our research aims to overcome the challenge of making this effect sensitive enough to image neuronal activity. (edu.au)
D. Snapshot of a simulated "voltage sensitive dye" image of the activity in layer 2/3, in response to a stationary bar stimulus, illustrating a "virtual experiment" in the model. (biomedcentral.com)
Calcium imaging with fluorescent protein sensors is widely used to record activity in neuronal populations. (janelia.org)
Each retinotopic point is able to learn a `snapshot' of points in the visual image whose activity tends to coincide with that retinotopic point. (paulbourke.net)

A recent computational study confirmed that the ANEP dyes are affected only by the electrostatic environment and not by specific molecular interactions. (wikipedia.org)

Our recent research published in Nature Photonics , explores a new type of a high speed, high resolution and scalable voltage imaging platform created with the aim of overcoming these limitations - a diamond voltage imaging microscope. (edu.au)
A prototype of a diamond voltage imaging microscope constructed by physicists at the University of Melbourne. (edu.au)
The following discussion of laser beam characteristics is presented as a general overview of the subject that may prove useful in employing lasers in microscope imaging, laser trapping, and other applications. (fsu.edu)

Confocal fluorescence microscopy images of phospholipid bilayers supported by gallium phosphide nanowires. (lu.se)

jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths. (janelia.org)
The physical response of the human eye differs for light of different wavelengths, and this has a bearing on the potential damage that may occur for several reasons that will be discussed below. (microscopyu.com)

Our lab has focused on the measurement of contrast-response and how it changes with attention in humans. (visionsciences.org)
In contrast to the axonal origin of voltage-sensitive dye signals, hVOS signals represent predominantly post-synaptic responses. (stanford.edu)
A successful technique employed by some investigators to improve differential interference contrast ( DIC ) images produced with an argon ion laser light source is to position a circular glass wedge, spinning at 2500 revolutions per minute, in the light path. (fsu.edu)

Sensitised transparency materials consist of an essentially random distribution of 'grains' which are chemically processed to reveal the dye couplers in different layers of the film. (naturephotographers.net)

These optogenetic techniques achieve unprecedented precision by introducing light-sensitive proteins into specific cell populations, which generate a response when illuminated. (electrooptics.com)
Fluorescent proteins that are sensitive to calcium or voltage can be used to visualize this activation non-invasively. (newport.com)
Unfortunately, fluorescent proteins are not sensitive to dynamic changes in the cell and cannot directly influence the cellÕs or organism's behavior. (newport.com)

Imaging Voltage Globally and in Isofrequency Lamina in Slices of Mouse Ventral Cochlear Nucleus. (stanford.edu)
Imaging hVOS signals in these slices recapitulated the tonotopic organization. (stanford.edu)

Both these transforms are mappings of the hemi-retinal image, and are achieved by known connectivity in the visual system. (paulbourke.net)

The fast response probe can be used for preliminary analysis of physiological processes with instantaneous (millisecond) potential changes, such as nerve impulses. (creative-bioarray.com)

While there are no known cases of a laser beam contributing to a person's death, there have been several instances of deaths attributable to contact with high voltage laser-related components. (microscopyu.com)
Electric voltage of 380 volts or less is considered low voltage and above 380 volts, high voltage. (medscape.com)
High voltage is generated at the power plant and is transformed down to approximately 120 volts for most wall outlets in homes. (medscape.com)
Electricity is transmitted by a high-voltage system because it allows the same amount of energy to be carried at lower current, which reduces electrical loss through leakage and heating. (medscape.com)

New voltage dyes can sense voltage with high speed and sensitivity using photoinduced electron transfer (PeT) through a conjugated molecular wire. (wikipedia.org)
ANNINE-6plus is a voltage sensitive dye with fast response (ns response time) and high sensitivity. (wikipedia.org)

This sensor array captures an image in 12 'bits' of data - that's 4096 different shades of grey, from 0 (Black) to 4095 (White). (naturephotographers.net)

We study the response of a liquid crystal-magnetic nanoparticle (LC-MNP) composite to applied potentials of hundreds of volts per meter. (aip.org)
Measuring samples using X-ray diffraction (XRD) and imaging composites using magnetic resonance imaging (MRI), we demonstrate that electric potentials applied across centimeter scale LC-MNP composite samples can be detected using XRD and MRI techniques. (aip.org)
Progressive advances in imaging technology, especially in magnetic resonance imaging (MRI), have reduced the use of EP testing in clinical practice. (medscape.com)

Figure 1: Delayed capillary dilation and reduced capillary red blood cell flow in response to a hindlimb stimulus in 1- to 2-month-old pericyte-deficient Pdgfrb +/− mice. (nature.com)
C. Network response to a moving bar visual stimulus. (biomedcentral.com)
The Piezo1 and Piezo2 ion channels are known to open up response to the slightest mechanical stimulus. (phys.org)

Essentially, equipment will include specialized microscopes and imaging devices, and may include technical lamps or lasers. (wikipedia.org)
As neuroscientists develop new genetic light sensitive tools, high peak power fs lasers for two-photon activation have developed in parallel. (newport.com)

Digital sensors on the other hand are actually a single layer of light sensitive 'photosites' arranged in a rigid rectangular matrix and these 'sites' capture information by generating a tiny voltage proportional to the intensity of the light which is projected onto them. (naturephotographers.net)
I do not know what their game plan is - they are big on image sensors. (parts-ring.com)

Imaging with a voltage sensitive dye provided a contrasting global view of signals arising predominantly from unmyelinated axons creating a potential method for studying type II auditory nerve or DCN parallel fibers. (stanford.edu)

Antic & Zecevic 1995 J Neurosci 15:1392 and http://www.scholarpedia.org/article/Voltage-sensitive_dye ). (scholarpedia.org)

Szwed, 2005 ), sensitive to either slow (Slowly Adapting, SA) or fast (Fast Adapting, FA) time varying stresses. (frontiersin.org)
If the path length or coherence state of the laser beam fluctuates at a faster interval than the detector integration time (in effect, the video frame rate), the speckle and scattering artifacts disappear from the image. (fsu.edu)
After this experience, Amar came to the conclusion that the audio system measurement techniques of the time such as measuring distortion and frequency response were not effective ways to evaluate the goal of natural sound reproduction. (diwa-gbr.de)
A larger voltage exerts a greater force, which moves more electrons through the wire at a given rate of time. (medscape.com)

In response to a peripheral infection, innate immune cells produce pro-inflammatory cytokines that act on the brain to cause sickness behaviour. (bioseek.eu)

The core of this service is slow or fast response detection. (creative-bioarray.com)

This service can provide customers with some commercial indicators, as well as customized services based on reactive fluorescent dyes. (creative-bioarray.com)

Since the local mapping of the supragranular layers produces a simultaneous expansion and contraction of the current visual inputs, it accounts for both the local response properties for orientation preference and their global geometric organisation. (paulbourke.net)
Each local mapping (via interactions with the global mapping and other local maps) supplies additional response properties such as orientation preference. (paulbourke.net)
The response properties of V1 are well catalogued. (paulbourke.net)

One class of methods for studying neural circuits is called voltage imaging . (edu.au)

During processing, the unused light sensitive chemicals are removed, revealing and leaving behind only the dyes. (naturephotographers.net)

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