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  • gels
  • Gels with large pores are usually used in this process to eliminate any "sieving" effects, or artifacts in the pI caused by differing migration rates for proteins of differing sizes. (wikipedia.org)
  • Coomassie blue-stained 2D PAGE gels of total human serum proteins (Panel A) and the fraction depleted of Albumin and immunoglobulin (Panel B) using PureProteome™ Human Albumin/Immuno¬globulin Magnetic Beads. (emdmillipore.com)
  • A high reproducibility in gel pore size and a high-yield electroelution of proteins performed by this highly reliable technique strongly correlate with the polymerization time of the acrylamide gels representing an inherent stability constant of the separation system. (wikipedia.org)
  • nucleic
  • MCSPs are extremely widespread in eubacteria (reference 8 and this study) and belong to the most conserved group of nucleic acid-binding proteins yet defined in nature: the cold shock domain (CSD) proteins ( 40 ). (asm.org)
  • eukaryotic
  • According to some opinions, living eukaryotic cells perform isoelectric focusing of proteins in their interior to overcome a limitation of the rate of metabolic reaction by diffusion of enzymes and their reactants, and to regulate the rate of particular biochemical processes. (wikipedia.org)
  • Eukaryotic initiation factor 4E (eIF4E) binding proteins (4E-BPs) regulate the assembly of initiation complexes required for cap-dependent mRNA translation. (asm.org)
  • The principal control point in mRNA translation is the initiation process, which is largely regulated through changes in the states of phosphorylation of proteins (eukaryotic initiation factors [eIFs] and other components) involved in this process (for reviews see references 11 , 31 , 34 ). (asm.org)
  • monomeric
  • An actin protein is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. (wikipedia.org)
  • temperature
  • This has led to the suggestion that the cold shock response is an adaptive mechanism facilitating protein synthesis at low temperature ( 25 ) and, as reported recently, at early exponential-phase growth ( 4 ). (asm.org)
  • In contrast to the relatively minor level of induction (2- to 10-fold) observed for most cold shock proteins during a temperature downshift in E. coli , the induction of a novel protein (initially designated as F10.6) was found to be considerably higher ( 24 ). (asm.org)
  • Optimal conditions for protein crystallization include optimal protein concentration in the saturated solution, pH and temperature among others whereas these conditions are determined by extensive trial and error experiments. (google.com)
  • After ascent, the Flash Evaporator System for cooling the orbiter iced up and shut down, increasing the crew cabin temperature to approximately 87 °F (31 °C). The problem was resolved on Flight Day 4 and cooler temperatures resulted. (wikipedia.org)
  • biomolecules
  • The present invention relates to methods and apparatus for promoting rapid formation of biomolecule crystals from a solution of biomolecules, preferably proteins, wherein the protein solution undergoes rapid concentration according to its isoelectric point in an electric field. (google.com)
  • detection
  • This technique facilitates the analysis of the relative amounts of proteins in milk, as well as simplifies the detection of changes and foreign proteins in milk. (calpoly.edu)
  • In principle, eastern blotting is similar to lectin blotting (i.e. detection of carbohydrate epitopes on proteins or lipids). (wikipedia.org)
  • typically
  • Crystal formation is a slow, and tedious process typically based on preparation of a saturated protein solution following nucleation, at optimal conditions. (google.com)
  • method
  • opted to refer to their method of drawing SDS-gel separated proteins onto nitrocellulose using a vacuum as Vacuum blotting. (wikipedia.org)
  • bound
  • How dimer-1 interacts with dimer-2 in the whole protein, however, depends on the exact combination of bound oxygens (white balls). (youtube.com)
  • Straub continued to work on actin, and in 1950 reported that actin contains bound ATP and that, during polymerization of the protein into microfilaments, the nucleotide is hydrolyzed to ADP and inorganic phosphate (which remain bound to the microfilament). (wikipedia.org)
  • modification
  • The presence of additional isoenzymes in the carcinoma , and dissimilarity of tryptic-digest patterns, may reflect an alteration in gene expression or in the post-translational modification of this protein in this heterogeneously differentiated transplantable pancreatic acinar carcinoma . (curehunter.com)
  • binds
  • eIF4E also interacts with the scaffold protein eIF4G, which in turn binds RNA helicase eIF4A to form the eIF4F complex ( 32 ). (asm.org)
  • Hemoglobin is a 4-subunit protein (a tetramer) that binds and transports oxygen. (youtube.com)
  • gene
  • Inverse PCR was used to amplify major cold shock protein (MCSP) gene families from a diverse range of bacteria, including the psychrotolerant Yersinia enterocolitica , which was found to have two almost identical MCSP coding regions ( cspA1 and cspA2 ) located approximately 300 bp apart. (asm.org)
  • major
  • This 70-amino-acid polypeptide was shown to be induced 200-fold following a shift from 37 to 10°C ( 24 ) and was subsequently termed the major cold shock protein (MCSP) or CspA ( 10 ). (asm.org)
  • Protein crystallization has three major applications: (1) structural biology and drug design, (2) bioseparations, and (3) purification. (google.com)
  • Isoelectric focusing of both normal and tumour alpha-amylases resolved each into two major isoenzymes , with pI 8.3 and 8.7. (curehunter.com)
  • The major bovine caseins (α s1 , α s2 , β, κ-casein) were resolved better on urea-PAGE. (calpoly.edu)
  • cellular
  • Cellular differentiation is characterized by coordinate induction of a repertoire of cell-specific proteins necessary for specialized functions. (ahajournals.org)
  • fraction
  • A short selection of proteins associated to the chloroplast envelope fraction was checked after superficial treatments of intact chloroplasts. (frontiersin.org)