• Biofilm formation on Candida auris , C. albicans , and C. glabrata yeast strains. (cdc.gov)
  • It simply does not help the child and increases the risk of breeding more resistant strains of bacteria," he said. (sciencedaily.com)
  • Any drug impairing crucial processes for bacterial life will inevitably lead to the development of drug-resistant strains, whereas the inhibition of biofilm formation might prevent the onset of bacterial resistance. (intechopen.com)
  • In this section, we will focus on proteins involved in biofilm formation as useful targets for the development of new drugs that can effectively and specifically impair biofilm formation with slight effects on cell survival, thus avoiding the generation of drug-resistant strains. (intechopen.com)
  • The MBL genes, bla IMP and bla VIM , were finally identified using the polymerase chain reaction (PCR) within imipenem-resistant strains. (japsonline.com)
  • About 69% of the strains were MDR, and 86.27% of the multi-drug resistant (MDR) strains were biofilm producers. (japsonline.com)
  • PCR showed that eight strains of imipenem-resistant P. aeruginosa contained bla VIM , while bla IMP gene was not detected. (japsonline.com)
  • This study aimed to isolate and identify P. aeruginosa from Al-Azhar University Hospital, identify the measurement of antibacterial activity of different groups of antibiotics on P. aeruginosa , determine the incidence of multi-drug resistant (MDR) P. aeruginosa in infected patients, and detect MBLs genes among MDR P. aeruginosa strains using polymerase chain reaction (PCR). (japsonline.com)
  • Chlorine was ineffective as experiments showed that strains V590, V595 and V. parahaemolyticus LMG 2850 could form biofilms even in the presence of 4 mg/ℓ of chlorine. (scielo.org.za)
  • When ozone was used, biofilm initiation and formation were completely inhibited for only 2 strains of V. alginoluticus , i.e. (scielo.org.za)
  • Since antibiotics are becoming increasingly ineffective in the treatment of biofilm-associated infections, the search for new compounds with anti-biofilm properties are urgently need.Methods: this study investigates the inhibitory effect of four phenolic compounds (named F1-F4 for simplicity) prior-to and post-biofilm formation by methicillin-sensitive S. aureus (MSSA) and MRSA strains related to the USA300 and USA100 clones. (conicet.gov.ar)
  • It is important to note that F3 (N-(3-cyano-4,5,6,7-tetrahydrobenzo [b] thiophen-2-yl)-3-hydroxybenzamide) presented a significant biofilm inhibitory effect by 77% to 23% in all but one strains under scrutiny. (conicet.gov.ar)
  • No compound affected the formation of biofilm by AR77, a MSSA strain of bovine origin.Conclusions: post-exposure of S. aureus to F3 produced an anti-biofilm effect in most strains analysed. (conicet.gov.ar)
  • Using multidrug-resistant A. baumannii strains, an antibiotic susceptibility test was performed using the Gram-negative identification card of the Vitek 2 system (bioMérieux Inc., France), as well as an analysis of resistance genes, the effects of treatment with a light-emitting diode (LED) array using Radachlorin (RADA-PHARMA Co., Ltd., Russia), and transmission and scanning electron microscopy to confirm the biofilm-inhibitory effect of PDT. (ophrp.org)
  • A study examining the biofilm-forming ability of multidrug-resistant A. baumannii strains recently isolated from a clinical sample indicated that all resistant bacteria exhibited higher biofilm-forming ability than the standard A. baumannii strain ATCC 19606 [ 11 ]. (ophrp.org)
  • 7 Additionally, P. aeruginosa is intrinsically resistant to a variety of antibiotics and disinfectants and multi-drug resistant (MDR) strains have been identified. (rsc.org)
  • The viscoelastic properties of this biofilm contributes to its ability to evade phagocytic activities, to reduce the effectiveness of antibiotics, to develop antibiotic resistant strains, and to persist or reoccur in CF patients. (aiche.org)
  • Multidrug-resistant (MDR) and hypervirulent Klebsiella pneumoniae , particularly carbapenem-resistant strains (CR- Kp ) causing high mortality and morbidity, are critical concerns[ 1 ]. (biorxiv.org)
  • With widespread antibiotic resistance cropping up in many strains of infection-causing bacteria, developing out-of-the-box strategies to protect patients from bacterial biofilms has become a critical focus area for clinical researchers," said Wyss Institute Founding Director Donald Ingber , who is also the Judah Folkman Professor of Vascular Biology at Harvard Medical School and Boston Children's Hospital and Professor of Bioengineering at Harvard SEAS. (zmescience.com)
  • MELBOURNE, Florida, February 7, 2008-- QuoNova LLC, which is 88.7% owned by XL TechGroup, Inc., has generated preliminary in-vitro data indicating high efficacy of selected Quorum Sensing Blockers ("QSB") in inhibiting biofilm formation by MRSA (methicillin-resistant Staphylococcus aureus) strains and fungal organisms such as Candida sp. (blogspot.com)
  • Working with Boston University synthetic biologist James Collins, -Harvard-MIT graduate student Timothy Lu has designed a new, highly effective means of dispersing and killing the bacteria living in biofilms. (technologyreview.com)
  • Given that bacteria living in biofilms are metabolically resistant to antibiotics, this study makes a definitive, scientifically-based statement against the use of these drugs to treat children with chronic ear infections. (sciencedaily.com)
  • The aims of this study were to investigate the prevalence of integrons and biofilm formation among P. aeruginosa and A. baumannii isolates collected from ICU and non-ICU inpatients. (magiran.com)
  • 81% of P. aeruginosa isolateswere sensitive to piperacillin/tazobactam and ticarcillin, while 60% were resistant to third generation of cephalosporins. (magiran.com)
  • Furthermore, 23% of the A. baumannii and 12% of the P. aeruginosa isolates showed strong biofilm activity. (magiran.com)
  • The goal of this study was to characterize P. aeruginosa isolates and identify the prevalence rate of P. aeruginosa resistant to imipenem due to metallo-ßlactamase (MBL) genes. (japsonline.com)
  • Possession of nosocomial multi-drug resistant P. aeruginosa infections was significantly associated with past antibiotic therapy or catheterization (p (japsonline.com)
  • The binding of the positively charged aminoglycosides to the negatively charged biofilm matrix polymers of P. aeruginosa will delay the penetration of aminoglycosides, [ 13 ] while the penetration of fluoroquinolones occurs immediately and without delay. (medscape.com)
  • the effectiveness of fluoroquinolones on biofilm-growing P. aeruginosa is greater when compared with β-lactams, while both fluoroquinolones and β-lactams are less effective against biofilm-growing P. aeruginosa compared to planktonic cells. (medscape.com)
  • Here, we investigated bacterial attachment, cell alignment and biofilm formation of Pseudomonas aeruginosa on periodic nano-pillar surfaces with different pillar spacing. (rsc.org)
  • 4,5 For example, Pseudomonas aeruginosa , an organism well-known for its capacity to form biofilms, is an opportunistic pathogen and is one of the top three causes of opportunistic human infections, 6 causing nosocomial infections in catheter lines, or chronically infecting the lungs of cystic fibrosis patients. (rsc.org)
  • 3,6 Biofilm formation by P. aeruginosa is responsible for antimicrobial tolerance and causes major problems for treatment of infections. (rsc.org)
  • This study correlates the activity of the quorum sensing inhibitors with viscoelastic changes in the P. aeruginosa biofilm on the air-medium interface. (aiche.org)
  • Pseudomonas aeruginosa is a biofilm-forming opportunistic pathogen which causes chronic infections in immunocompromised patients and leads to high mortality rate. (frontiersin.org)
  • We collected a strong biofilm-forming P. aeruginosa strain displaying small colony variant morphology from a severe COVID-19 patient. (frontiersin.org)
  • To test the effectiveness of the super slippery surface, the study's lead author Noah MacCallum, an exchange undergraduate student at SEAS, exposed treated and untreated medical tubing to Pseudomonas aeruginosa , Escherichia coli , and Staphylococcus epidermidis , which are common pathogenic bacteria that form biofilms and are the most common culprits in blood and urinary infections. (zmescience.com)
  • CP-CRPA infections tilation during 3 of 5 weeks of hospitalization in an intensive are highly transmissible in health care settings because they care unit (ICU) room (room X). Carbapenemase-producing can spread from person to person and from environmental carbapenem-resistant Pseudomonas aeruginosa (CP-CRPA) was sources such as sink drains and toilets. (cdc.gov)
  • Carbapenem-resistant P. aeruginosa (CRPA) isolates in Idaho intensive care unit (ICU) room (room X), 4 months apart. (cdc.gov)
  • They found that at low concentrations, the compound, DAPG (the acronym stands for 2,4-diacetylphloroglucinol), produced by the bacterium Pseudomonas protegens, did not kill the experimental target bacterium, Bacillus subtilis, but merely prevented it from forming biofilms. (sciencedaily.com)
  • In her laboratory, LaPlante particularly studies biofilms in context of clinical infections, and focuses on the prevention and treatment of biofilm-associated infections in Staphylococcus, Enterococcus and Pseudomonas. (wikipedia.org)
  • Mapping transcriptional regulation of biofilm-related genes promoters in Pseudomonas. (usp.br)
  • More than 65 percent of hospital-acquired infections manifest as biofilms. (sciencedaily.com)
  • LaPlante conducted study regarding the role of daptomycin in the treatment of resistant Gram-positive infections, including skin and skin-structure infections resulted from surgery, diabetic foot ulcers, and burns. (wikipedia.org)
  • Bacterial biofilms play an important role in UTIs, responsible for persistent infections leading to recurrences and relapses. (mdpi.com)
  • Biofilms, which often form on hard surfaces such as catheters and water pipes, can cause gingivitis and chronic ear infections. (technologyreview.com)
  • Large doses of antibiotics can usually eradicate these infections, but there is some worry that drug--resistant biofilm infections are becoming more common. (technologyreview.com)
  • Infections caused by multidrug resistant (MDR) Klebsiella pneumoniae are difficult to treat with conventional antibiotics. (researchgate.net)
  • Direct evidence of bacterial biofilms has been found on the middle ear tissue of children who suffer from chronic ear infections, according to a study published today in the Journal of the American Medical Association (JAMA) by researchers from the Allegheny Singer Research Institute (ASRI) at Allegheny General Hospital in Pittsburgh, the Medical College of Wisconsin and Children's Hospital of Wisconsin in Milwaukee. (sciencedaily.com)
  • Over the past ten years, Dr. Ehrlich and J. Christopher Post, M.D., Ph.D., FACS, an Allegheny General Hospital pediatric ear specialist and medical director of the Center for Genomic Sciences, have pioneered the biofilm theory to explain the persistence of chronic ear infections. (sciencedaily.com)
  • The capacity of S. aureus to build biofilms is correlated with the severity of infections ( 4 ). (frontiersin.org)
  • We now report our seminal findings on the major constituents including terpenes identified in native, historically significant herbal medicinal plant Elder (Sambucus nigra L.) flower and elder berry in particular and their concomitant strong antimicrobial effects exhibited on various nosocomial pathogens notably upon methicillin-resistant Staphylococcus aureus MRSA, recognised globally as a clinically significant pathogen, associated with skin and soft tissue infections. (researchgate.net)
  • Background: biofilms are involved in chronic persistent infections, such as bovine mastitis, osteomyelitis and foreign body-related infections. (conicet.gov.ar)
  • Biofilms may be responsible, in part, of late reactivation of staphylococcal chronic infections after the initial disease has been cured. (conicet.gov.ar)
  • One of the most intriguing and clinically relevant features of microbial biofilms is their significantly higher antibiotic resistance relative to their free-floating counterparts, which generates serious consequences for therapy of biofilm-associated infections. (medscape.com)
  • In developed countries, over 60% of bacterial infections requiring treatment involve biofilm formation [ 12 , 13 ]. (ophrp.org)
  • 3 Bacterial biofilms can cause persistent human infections and can foul the surface of medical devices. (rsc.org)
  • 3,8 Therefore, it is important to develop biomaterials that can control biofilm growth thereby reduce infections. (rsc.org)
  • Therefore, since it is a global public health problem involving several sectors, it also requires a global solution in the context of the One Health approach to achieve adequate control through the prevention, reduction, and mitigation of drug-resistant infections. (who.int)
  • It is a biofilm-forming opportunistic pathogen causing life-threatening chronic infections in immunocompromised individuals with diseases like burn wounds, urinary tract infections, and respiratory infections ( Gellatly and Hancock, 2013 ). (frontiersin.org)
  • Antibiotic-resistant bacteria are a growing problem in the 21st century, causing longer and more serious infections, and resulting in poor outcomes for vulnerable patients. (qub.ac.uk)
  • It is envisaged that these dual-functional materials will be capable of inhibiting biofilm formation when deployed as a coating on medical devices, protecting patients from bacterial infections, while simultaneously providing visual feedback to healthcare professionals if the material has reached the end of its anti-infective lifespan. (qub.ac.uk)
  • As a result of biofilm formation of (resistant) bacteria on the implant, more implant-associated infections (IAI) occurred. (tudelft.nl)
  • However, sutures can harbor bacteria, leading to the formation of biofilms and related infections, which can be problematic to treat. (materialstoday.com)
  • We are working to better understand what microbes and their surrounding environments can teach us about preventing infections, including antimicrobial-resistant infections . (cdc.gov)
  • In fact, it was shown that the majority of all bacterial infections are related to biofilm growth, stressing the importance of this life style [ 2 ]. (biomedcentral.com)
  • Harvard researchers have demonstrated a powerful, long-lasting repellent surface technology that can be used with medical materials to prevent infections caused by biofilms. (zmescience.com)
  • The results give great hope for future applications and reducing infections, especially with drug-resistant bacteria. (zmescience.com)
  • M chelonae, along with M abscessus, are considered the most drug resistant of the NTM group, which leads to difficulty when treating infections these organisms. (medscape.com)
  • 2) The direct bactericidal activity of HAMLET, a human milk protein-lipid complex, against various bacterial species as well as its adjuvant activity in sensitizing bacteria to a broad range of common antibiotics to provide novel therapeutic strategies against respiratory and other infections with antibiotic-resistant bacteria. (lu.se)
  • She has also explored pharmacodynamic dosing indexes of antimicrobial therapy and the role of drug therapy on bacterial virulence factors such as toxin and biofilm formation. (wikipedia.org)
  • The microbial community is also a natural source of metabolites and has the potential to be used to create antimicrobial and anti-biofilm agents. (frontiersin.org)
  • The disk diffusion method was applied to determine antimicrobial resistance patterns according to the Clinical and Laboratory Standards Institute, and the microtiter plate assay was managed to explore the biofilm formation. (japsonline.com)
  • An evidence-based scientific scrutiny of Irish traditional medicines for their antimicrobial potency is urgently required for combating antibiotic resistant common nosocomial pathogens. (researchgate.net)
  • 1,2 The unique structure of biofilms protects bacteria from the surrounding environment, conferring a capacity for persistence against phagocytosis, oxidative stresses, nutrient/oxygen restriction, metabolic waste accumulation, interspecies competition, and conventional antimicrobial agents. (rsc.org)
  • Antimicrobial resistance occurs through different mechanisms, which include spontaneous (natural) genetic mutations and horizontal transfer of resistant genes through deoxyribonucleic acid (DNA). (who.int)
  • Antimicrobial resistant microorganisms are found in people, animals and the environment and can spread globally. (who.int)
  • In this work, we studied the antimicrobial resistance and performed a comparative genomics analysis of ten CR- Kp isolates from the Chilean surveillance of carbapenem-resistant Enterobacteriaceae . (biorxiv.org)
  • Furthermore, the exponential increase in microbes resistant to antibiotics or antibacterial agents has pushed research to look for alternative substances with antimicrobial properties. (materialstoday.com)
  • The researchers' findings indicate that spider silk proteins functionalized with antimicrobial peptides can be used to create an effective coating for commercial silk sutures to reduce, prevent, or eliminate bacterial adhesion and biofilm formation, which lead to infection. (materialstoday.com)
  • Un 40% de esos trabajos han sido publicados en revistas del primer cuartil entre las que destacan New England Journal of Medicine, Lancet, Lancet Infectious Diseases, Clinical Infectious Diseases, Clinical Microbiology Reviews, Antimicrobial Agents and Chemotherapy, Journal Clinical Microbiology, Journal Antimicrobial Chemotherapy, Mayo Clinic Proceedings y Malaria Journal. (unav.edu)
  • These natural products find application against resistant pathogenic microorganisms without the risk of antimicrobial resistance acquisition and prevent the formation or distortion of biofilms. (benthamscience.com)
  • The water samples were di- of inhibition of each antimicrobial agent bacterium survives in water systems as rectly placed in ice, for transportation was measured and recorded as resistant, a parasite of protozoa [4], which are and examination within the same day. (who.int)
  • Biofilms are notoriously resistant to antibiotics. (sciencedaily.com)
  • When bacteria team up in sticky communities called biofilms, they can be nearly impossible for conventional antibiotics to eradicate. (technologyreview.com)
  • Because they are protected by a sticky carbohydrate scaffold called a matrix, bacteria living communally in biofilms are a thousand times as resistant to antibiotics as free--swimming bacteria are, says Collins. (technologyreview.com)
  • What's more, some evidence suggests that the use of antibiotics actually induces biofilm formation. (technologyreview.com)
  • Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)
  • [13] The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
  • Though antibiotics have proven to be effective for children with acute OM where biofilms have not yet formed, those with chronic disease typically benefit little from the drugs and more so from myringotomy, a surgical procedure in which small tubes are placed in the eardrum to continuously drain infectious fluid (called effusion). (sciencedaily.com)
  • In case of A. baumannii, all the isolates were sensitive to colistin, but 98% were resistant to other antibiotics (p≤0.05). (magiran.com)
  • Class 1 integron-positive isolates were resistant to three classes of antibiotics and predominantly observed in specimens collected from ICU patients showing strong biofilm. (magiran.com)
  • The MIC of antibiotics to biofilm-growing bacteria may be up to 1000-fold higher than that of planktonic bacteria. (medscape.com)
  • The biofilm matrix can act as a barrier to delay the diffusion of antibiotics into biofilms [ 11 ] because antibiotics may either react chemically with biofilm matrix components or attach to anionic polysaccharides. (medscape.com)
  • [ 12 ] Antibiotics have been shown to readily penetrate biofilms in some cases, but poorly in others depending on particular antibiotics and biofilms. (medscape.com)
  • thereby, the slow growth rates of biofilm-growing cells will render them less susceptible to antibiotics. (medscape.com)
  • In the laboratory, bacteria become highly resistant to antibiotics when nutrients are limited in the media. (medscape.com)
  • That's bad because mature biofilms are very resistant to antibiotics-just like antibiotic-resistant superbugs like MRSA. (stevens.edu)
  • Additional studies on the effective elimination of biofilms containing multidrug-resistant bacteria are necessary, and we hope that a treatment method superior to sterilization with antibiotics will be developed in the future. (ophrp.org)
  • The disruption of biofilm formation would have a beneficial impact on the treatment of diseased CF patient as it will make the infection more susceptible to antibiotics and host phagocytosis. (aiche.org)
  • We are short of new antibiotics, and infection caused by resistant bacteria (i.e. superbugs) may lead to another pandemic. (hku.hk)
  • Because of the protected environment, bacteria within biofilms are less sensitive to environmental stresses, including disinfectants and antibiotics, and are as a consequence very difficult to eradicate [ 3 ]. (biomedcentral.com)
  • In addition, bacteria form a biofilm on and within the endotracheal tube that protects them from antibiotics and host defenses. (msdmanuals.com)
  • In this study the effectiveness of chlorine, ozone and hydrogen peroxide (H 2 O 2 ) in the inhibition of mature biofilms or biofilm formation in natural seawater was investigated. (scielo.org.za)
  • The prior-to-exposure evaluation revealed that F2 induced 89% to 39% biofilm inhibition showing higher activity on MSSA from bovines than those S. aureus isolates from humans. (conicet.gov.ar)
  • This study aimed to test the effect of photodynamic therapy (PDT) on the inhibition and removal of biofilms containing multidrug-resistant Acinetobacter baumannii . (ophrp.org)
  • Bacteria can be found in planktonic form or in specific conditions, as sessile aggregates on both biotic and abiotic surfaces originating complex structures known as biofilm. (intechopen.com)
  • Multidrug and carbapenem-resistant K. pneumoniae (CR- Kp ) are considered critical threats to global health and key traffickers of resistance genes to other pathogens. (biorxiv.org)
  • This disrupts our microbiome, allowing the surviving pathogens, which are often resistant to the treatment, to dominate our microbiome. (cdc.gov)
  • Some of the surviving pathogens are often resistant to the treatment. (cdc.gov)
  • When a healthcare facility identifies certain multidrug-resistant pathogens within their facility, CDC recommends colonization screening-using laboratory test to identify patients who are colonized. (cdc.gov)
  • VAP often involves more resistant pathogens and poorer outcomes than other forms of hospital-acquired pneumonia. (msdmanuals.com)
  • While the function of lytic transglycosylases (LTs) in relation to cell division , biofilm formation, and antibiotic resistance has been determined for several bacteria , their role in S. aureus remains largely unknown. (bvsalud.org)
  • Our study demonstrates that, in a strain of methicillin-resistant S. aureus ( MRSA ), IsaA and SceD contribute differently to biofilm formation and ß- lactam resistance. (bvsalud.org)
  • We report here that myricetin, but not its glycosylated form, can remarkably decrease the production of several S. aureus virulence factors, including adhesion, biofilm formation, hemolysis and staphyloxanthin production, without interfering with growth. (nature.com)
  • This study focused on the changes in biofilm activity and related metabolic pathways of S. aureus treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS). (frontiersin.org)
  • Additionally, it inhibits the physiological traits of the S. aureus biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. (frontiersin.org)
  • The metabolites of S. aureus biofilm treated with LAB-CFS were greater in the LAB-bf-CFS than they were in the LAB-pk-CFS, according to metabolomics studies. (frontiersin.org)
  • S. aureus produces large biofilm formations that support its pathogenicity and confer protection and subsequently drug resistance ( 5 ). (frontiersin.org)
  • The differentially expressed genes and proteins linked to S. aureus biofilms have been identified using transcriptomic and proteomic investigations ( 14 - 17 ). (frontiersin.org)
  • Because of this, it is crucial to understand how LAB-CFS affects the makeup and function of metabolites in S. aureus biofilms. (frontiersin.org)
  • Here, we used untargeted metabolomics to investigate the potential impacts of planktonic bacteria (LAB-pk-CFS) and biofilm colonies (LAB-bf-CFS) on the metabolism of S. aureus biofilms. (frontiersin.org)
  • The increasing prevalence of methicillin-resistant S. aureus (MRSA) and the emergence of vancomycin-resistant S. aureus have created major problems to the Public Health. (conicet.gov.ar)
  • The mature biofilms were more resistant to H 2 O 2 but were all eliminated at 0.2% concentrations. (scielo.org.za)
  • For the study, first author Matthew Powers, an undergraduate student in Shank's lab, used a strain of B. subtilis--a species commonly used in lab experiments--that fluoresces when genes for biofilm formation are being shut off. (sciencedaily.com)
  • [10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)
  • In this sense, we aim to characterize the behaviour of different promoters of genes involved in biofilm regulation under different conditions and to search for possible new DNAbinding proteins associated with their promoter regions. (usp.br)
  • Next to UFA biosynthesis, a number of these direct targets and other indirect targets identified by transcriptomics (e.g. ribosomal genes, ompA , ompC , ompX , osmB , osmC, sseI ), could possibly contribute to the effect of FabR on biofilm formation. (biomedcentral.com)
  • Silk sutures were simply dip-coated with the bioengineered 6mer-HNP1 spider silk coating and tested against Gram-positive and Gram-negative bacteria, fungi, viruses, and multi-resistant organisms such as MRSA. (materialstoday.com)
  • Pathogenic characteristics such as germ tube and biofilm formation and production of tissue damaging enzymes are possible targets of new drugs. (hindawi.com)
  • However, there have not been many investigations on how pathogenic bacteria are affected by the metabolic properties of planktonic and biofilm probiotics. (frontiersin.org)
  • For many bacteria, biofilm formation is a key part of their pathogenic behaviour, and is especially important in the development of medical device-associated infection. (qub.ac.uk)
  • The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)
  • Biofilms are an ensemble of microbial cells irreversibly associated with a surface and enclosed in an essentially self-produced matrix. (intechopen.com)
  • Bacterial cells can colonize surfaces and form biofilms that consist of microbial cells embedded in extracellular polymeric substances (EPS). (rsc.org)
  • Historically, multicellular bacterial communities, known as biofilms, have been thought to be held together solely by a self-produced extracellular matrix. (nature.com)
  • Biofilms are multicellular communities that were so far thought to be held together solely by a self-produced organic extracellular matrix. (nature.com)
  • Differential gene expression analysis indicated that this isolate formed excessive biofilm by reducing flagellar formation (7.4 to 1,624.1 folds) and overproducing extracellular matrix components including CdrA (4.4 folds), alginate (5.2 to 29.1 folds) and Pel (4.8-5.5 folds). (frontiersin.org)
  • A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
  • Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
  • The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
  • Isolates were standardized to 10 6 cells/mL in RPMI-1640 and grown in flat-bottomed 96-well microtiter plates for 24 h at 37°C. Biofilms were then washed, stained with crystal violet solution, and quantified. (cdc.gov)
  • In summary, we demonstrated that P. aeuginosa clinical isolates with novel epigenetic markers could form excessive biofilm, which might enhance its antibiotic resistance and in vivo colonization in COVID-19 patients. (frontiersin.org)
  • Biofilm formation requires three different stages: cell attachment to a solid substrate, adhesion, and growth. (intechopen.com)
  • The experiment confirmed what scientists believed - that the surface greatly reduces biofilm adhesion and largely (though not totally) eliminated biofilm formation. (zmescience.com)
  • Additionally, the experimental approach could be used to discover other, potentially medically important biofilm-inhibiting bacterial, said Shank. (sciencedaily.com)
  • Biofilm is a complex matrix consisting of extracellular polysaccharides, DNA, and proteins that protect bacteria from a variety of physical, chemical, and biological stresses allowing them to survive in hostile environments. (intechopen.com)
  • Bacteria with high biofilm-forming ability attach to host cells more easily or colonize medical devices and hospital environments, promoting horizontal gene transfer between bacteria in biofilms [ 14 , 15 ]. (ophrp.org)
  • Biofilm formation is an important survival strategy of Salmonella in all environments. (biomedcentral.com)
  • Knowledge about the complex regulatory processes in biofilm formation can provide more insight into survival strategies of S. Typhimurium in non-host environments and can be the fundament of new eradication methods. (biomedcentral.com)
  • These promising results, which are the subject of continuing research, further underscore the potential of QuoNova's QSB in combating the detrimental effects of biofilms, especially in clinical environments, where mixed microbial communities predominate. (blogspot.com)
  • It appears that in many cases recurrent disease stems not from re-infection as was previously thought and which forms the basis for conventional treatment, but from a persistent biofilm," Ehrlich said. (sciencedaily.com)
  • This can lead to infection, including a resistant infection. (cdc.gov)
  • Liquid-infused polymers could be used to prevent biofilms from ever taking hold, potentially reducing rates of infection and therefore reducing dependence on antibiotic use. (zmescience.com)
  • 1) Biofilm formation during bacterial colonization and how the composition of the respiratory microbiota as well as environmental factors (host inflammation, virus infection) modulate or trigger transition from colonization to infection, such as otitis media, pneumonia and sepsis. (lu.se)
  • However, ground squirrels and prairie dogs have been known to be highly susceptible to plague, whereas others have been known to be either moderately susceptible or absolutely resistant to infection. (medscape.com)
  • A known antibiotic and antifungal compound produced by a soil microbe can inhibit another species of microbe from forming biofilms - -microbial mats that frequently are medically harmful -- without killing that microbe. (sciencedaily.com)
  • DAPG, or the DAPG-producing P. Protogens as a protobiotic, could be used to inhibit formation of harmful biofilms. (sciencedaily.com)
  • In particular, surface modifications that physically create rational surface topographies have attracted attention in recent years, and have shown to inhibit bacterial attachment and biofilm growth without the use of antimicrobials. (rsc.org)
  • Targeting these proteins with multivalent glycosides can inhibit biofilm-formation by crosslinking these lectins. (qub.ac.uk)
  • Results show that C. auris can form heterogeneous intermediate biofilms. (cdc.gov)
  • Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
  • [8] Biofilms can form on the teeth of most animals as dental plaque , where they may cause tooth decay and gum disease . (wikipedia.org)
  • A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)
  • Biofilms are antibiotic resistant colonizations of bacteria that attach to surfaces and form a slime-like barrier that acts as a formidable defense mechanism, protecting the bacteria from eradication. (sciencedaily.com)
  • It is likely that, in the presence of F3, certain bacterial cells are able to attach and form biofilms, but their maturation process is significantly hampered. (conicet.gov.ar)
  • The 6mer-HNP1 coating appears to reduce the ability of bacteria to stick to the surface of silk sutures and form biofilms. (materialstoday.com)
  • Such bacterial biofilms tend to form on medical equipment, including surgery equipment heart valves, urinary catheters, intravenous catheters, and implants. (zmescience.com)
  • Thus, the aim of this research was to determine whether yeast present in CVC colonizations previously exposed to cell- wall targeted antibacterials benefit from a reduction in susceptibility to fluconazole and voriconazole, facilitating their ability to form biofilms. (bvsalud.org)
  • None of the antibacterials exerted a significant effect on the in vitro susceptibility of the yeasts to the antifungal agents or on their ability to form biofilms. (bvsalud.org)
  • A biofilm is any group of microbes that stick together on a surface. (sciencedaily.com)
  • Bacteria predominantly grow inside multicellular communities attached to solid surfaces and enclosed in a self-produced polymeric matrix, called biofilms [ 1 ]. (biomedcentral.com)
  • It was shown for example that an incomplete LPS fraction does not affect biofilm formation capacity of Salmonella on hydrophobic gallstone surfaces, but highly reduces its biofilm capacity on hydrophilic glass surfaces [ 7 ]. (biomedcentral.com)
  • It is hydrophobic and adheres to surfaces, owing to the structure and make up of its mycolic acids resulting in biofilm formation. (medscape.com)
  • Multi-drug resistant was found in 29.4% participants. (preprints.org)
  • The chloromelamine-based fibrous materials provided potent, durable, and rechargeable biocidal functions against bacteria (including multi-drug resistant species), yeasts, viruses, and bacterial spores. (cdc.gov)
  • The inhibitory effect of compounds on biofilms was visualized by scanning electron microscopy.Results: the initial screening using the Newman strain showed the phenolic compounds as inactive (F1), moderately active (F2, F4) or highly active (F3). (conicet.gov.ar)
  • Overall, our results point at the importance of FabR and UFA biosynthesis in Salmonella biofilm formation and their role as potential targets for biofilm inhibitory strategies. (biomedcentral.com)
  • If such bacteria-attacking viruses prove safe for industrial and clinical use, researchers could develop stocks of different kinds of viruses, each tailored to attack a different kind of biofilm. (technologyreview.com)
  • 287: 1710 - 1715) the first animal evidence of biofilms in the middle ear, setting the stage for the current clinical investigation. (sciencedaily.com)
  • En mayo de 2007 obtuve el Certificate of Training in Molecular Biological Techniques en el Department of Molecular Biology y desde Junio de 2008 a Junio de 2009 completé el Certificate in Clinical Research en el Center for Translational Science Activities en Mayo Clinic College of Medicine, Rochester Minnesota. (unav.edu)
  • In the relentless battle against antibiotic-resistant superbugs, science continues to unveil ingenious strategies to address their vulnerability. (hku.hk)
  • Using advanced confocal laser scanning microscopy, three dimensional images were obtained of the biopsies and evaluated for biofilm morphology using generic stains and species-specific probes for Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis by Luanne Hall Stoodley, Ph.D. and her ASRI colleagues. (sciencedaily.com)
  • In order to unravel how this regulator impinges on Salmonella biofilm formation, we aimed at elucidating the S . Typhimurium FabR regulon. (biomedcentral.com)
  • Furthermore, the presence of flagella and fatty acid containing structures such as lipopolysaccharides was shown to be important in Salmonella biofilms [ 7 ]. (biomedcentral.com)
  • We studied, for the first time, the effects of mutants defective in biomineralization and calcite formation on biofilm development, resilience and morphology. (nature.com)
  • The biofilm matrix consists of polysaccharides, proteins, and DNA and constitutes a stubborn source that protects bacteria from a variety of physical, chemical, and biological stresses. (intechopen.com)
  • PDT involving a combination of LED and Radachlorin significantly eliminated the biofilm of multidrug-resistant A. baumannii . (ophrp.org)
  • These biofilms negatively impact water quality and could lead to contamination of fish products. (scielo.org.za)
  • F4 decreased biofilm production by 23% only in the Newman strain. (conicet.gov.ar)
  • As we found a fabB overexpressing strain to partly mimic the biofilm defect of the fabR mutant, the effect of FabR on biofilms can be attributed at least partly to FabB, which plays a key role in UFA biosynthesis. (biomedcentral.com)
  • The biofilm biomass was stained with crystal violet for spectrophotometric quantification. (conicet.gov.ar)
  • Biofilm quantification was done by microtiter method. (magiran.com)
  • [8] [5] The first colonist bacteria of a biofilm may adhere to the surface initially by the weak van der Waals forces and hydrophobic effects. (wikipedia.org)
  • A biofilm forms when bacteria adhere to a surface and proliferate into a colony that is very difficult to remove and can cause a lot of problems. (stevens.edu)
  • it even provides something like a primitive circulatory system, distributing nutrients to cells throughout the biofilm. (technologyreview.com)
  • [ 20 ] Starvation is also found in biofilms owing to nutrient consumption by peripheral cells and reduced diffusion of oxygen and nutrients through biofilms. (medscape.com)
  • [ 10 ] Multiple biofilm-specific mechanisms are operated simultaneously in a reversible and transient manner contributing to the high levels of antibiotic resistance of biofilms, and these are distinct from the well-characterized intrinsic resistance mechanisms (e.g., expression of antibiotic-degrading enzymes, inducible decrease in antibiotic influx, inducible increase in antibiotic efflux and alteration in antibiotic target sites) employed by planktonic cells. (medscape.com)
  • The susceptibility to fluconazole and voriconazole and the biofilm formation of the yeasts were tested before and after exposure to the antibacterials. (bvsalud.org)
  • The discovery of biofilms in the setting of chronic otitis media represents a landmark evolution in the medical community's understanding about a disease that afflicts millions of children world-wide each year and further endorses the emerging biofilm paradigm of chronic infectious disease, said Garth Ehrlich, Ph.D., principal investigator and executive director of the ASRI Center for Genomic Sciences. (sciencedaily.com)
  • Nearly all of the children in our study who suffered from chronic otitis media tested positive for biofilms in the middle ear, even those who were asymptomatic. (sciencedaily.com)
  • They then insert a new gene into the virus, one that codes for an enzyme that dissolves the main carbohydrate component of the biofilm matrix. (technologyreview.com)
  • Subpopulations of cells within the biofilm differentiate to perform various activities for motility, matrix production, and sporulation, supporting the overall success of the biofilm. (wikipedia.org)
  • Moreover, this study demonstrates that the degrees of antibiotic resistance and biofilm production are directly linked. (japsonline.com)
  • If the time required for an antibiotic to penetrate biofilms is longer than the duration of antibiotic treatment, the slower penetration will explain the antibiotic resistance. (medscape.com)
  • Any synthetic surface that touches the body, from an artificial heart to a hip implant to a cochlear hearing aid can catalyze biofilm formation. (stevens.edu)
  • Surface topography designed to achieve spatial segregation has shown promise in delaying bacterial attachment and biofilm growth. (rsc.org)
  • 2,9-11 A comprehensive understanding of the interactions between bacteria and materials with different surface topographies may pave the way for more effective strategies to control biofilm growth. (rsc.org)
  • Biocidal efficacy, biofilm-controlling function, and controlled release effect of chloromelamine-based bioresponsive fibrous materials. (cdc.gov)