Residues within proteins amino acids in the protein protein residues. Medical search. Frequent questions

Copper ions bind to biomolecules (e.g., peptides and proteins) playing an essential role in many biological and physiological pathways in the human body. (mdpi.com)
The trypsin catalyses only the hydrolysis of only alpha amino peptides, i.e. (physicsforums.com)

To test this, we aligned 97 protein sequences consisting of homologs of UMAMIT clade I from 27 plant species and created sequence logos containing the 51 residues identified in the structural analysis ( Supplementary Figure 1 ). (frontiersin.org)
The idea behind the latest work 1 , 2 is simple - model the transition state for a reaction, stabilize it by surrounding it with carefully placed chemical groups, graft the resulting active site into an existing protein and then alter the amino-acid sequence of the protein to accommodate the changes. (nature.com)
The design process involved modifying the amino-acid sequence of a naturally occurring protein. (nature.com)
Based on primary sequence comparisons, β subunits are predicted to be modular structures composed of five domains (A-E) that are related to the large family of membrane-associated guanylate kinase proteins. (jneurosci.org)
One pathway, which has been extensively studied in yeast, is mainly guided by chromatin structure and the other, analyzed in detail in mice, is driven by the sequence-specific DNA-binding PR domain-containing protein 9 (PRDM9). (springer.com)
By default, Rosetta's packer , which is used for side-chain rotamer optimization and for sequence design, does not design with D-amino acids, but they can be turned on using the "NC" (for "non-canonical") command in a resfile. (rosettacommons.org)
2009 ). The designated dysbindin paralogs show very limited sequence homology which raised the question whether DBNDD1 and DBNDD2 are dysbindin-like proteins or proteins that share a less conserved domain with DTNBP1 in the context of otherwise unrelated sequences (Ghiani and Dell'Angelica 2011 ). (springer.com)
In contrast, isoform 3 (UniProtKB: Q9H9R9-3) carries a 100 amino acids long N-terminal sequence extension. (springer.com)
As an outcome human DBNDD1 revealed a high sequence identity to dysbindin domain-containing proteins from other Hominidae (e.g. (springer.com)
Proteins with high sequence identity to human DBNDD1 can also be found in evolutionarily more distant species (e.g. (springer.com)
A number of eukaryotic and archaebacterial large subunit ribosomal proteins can be grouped on the basis of sequence similarities. (embl.de)
The proposed method is novel because it works independently of protein sequence databases and without any prior knowledge about modifica- tions. (lu.se)
The matching is com- plicated by the fact that there are protein modifications and the sequence databases store the unmodified sequences. (lu.se)
PTMs from this list in conjunction with a protein sequence and a few precursor masses. (lu.se)

Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
Eotaxin-2 contains the four conserved cysteine residues present in CC chemokines. (peprotech.com)

A globular protein containing approximately 200 amino acid residues was crystallised. (edurev.in)
Find important definitions, questions, meanings, examples, exercises and tests below for A globular protein containing approximately 200 amino acid residues was crystallised. (edurev.in)
Solutions for A globular protein containing approximately 200 amino acid residues was crystallised. (edurev.in)
The TULP proteins share a conserved carboxy-terminal region of approximately 200 amino-acid residues. (nih.gov)

Moreover, single amino acid substitutions at Asp123, Glu124, or Glu125 in the C-terminal region of Mms6 clearly indicated that these amino acid residues had a direct impact on magnetite crystal morphology. (elsevierpure.com)
Unexpectedly, all ten amino acid substitutions marginally reduce protein thermostability. (rcsb.org)
in December 2006, UniMod lists 495 modifications including 144 amino acid substitutions [6]. (lu.se)

d ) A sectional view through the map and superimposed model, showing details of the central transmembrane pore (functionally important amino acid residues on the α -subunit pore-lining M2 helices, 9′Leu, uppermost, and −1′Glu, are shown in red). (iucr.org)
We believe that the present results resolve the long-standing controversy regarding the mechanism behind the spin relaxation dispersion of water nuclei in protein solutions, thus establishing oxygen-17 relaxation as a powerful tool for studies of structurally and functionally important water molecules in proteins and other biomolecules. (lu.se)

In addition, mutations of any of the cysteine residues of EGF in PE(ΔIa)-EGF mutants resulted in the loss of their binding activity to EGF receptor and a corresponding loss of their cytotoxicity. (tmu.edu.tw)
Either at genomic or at proteomic level, mutations have significant impact on normal gene or protein function, and human diseases could be associated with mutations like nonsynonymous single-nucleotide variations (nsSNVs) on amino acids. (hindawi.com)
Yet how gene mutations affect protein activities through posttranslational modification sites have not been widely studied. (hindawi.com)
The observed conformational changes illustrate a general mechanism by which proteins can tolerate mutations. (rcsb.org)
These mutations lead to an altered apoA-I protein. (medlineplus.gov)
Mutations in the APOA1 gene can also cause a condition called familial visceral amyloidosis, which is characterized by an abnormal accumulation of proteins (amyloidosis) in internal organs (viscera). (medlineplus.gov)
The mutations that cause this condition alter the apoA-I protein. (medlineplus.gov)

Recombinant protein corresponding to amino acid residues 1-206 of murine p59fynprotein. (thermofisher.com)
Anti·His HRP Conjugate Kits, including the RGS·His HRP Conjugate Kit (His tag conjugates) and Penta·His HRP Conjugate Kit (6xHis tag conjugates), are intended for sensitive detection of recombinant proteins carrying His tags, without the need for secondary antibodies. (qiagen.com)
They can be used for highly specific and sensitive, direct detection of any recombinant protein that has an accessible His tag. (qiagen.com)
Recombinant Murine Neuropoietin is a 19.8 kDa protein containing 183 amino acid residues. (peprotech.com)
Recombinant mouse IL-1β is useful as a quantitative standard for measuring mouse IL-1β protein levels in an IL-1β -specific sandwich ELISA. (bdbiosciences.com)
Recombinant Human Eotaxin-2 is an 8.8 kDa protein containing 78 amino acid residues. (peprotech.com)

Based on a predicted model of UMAMIT29, we propose that the substrate transporting cavity consists of 51 residues, of which four are highly conserved residues across all the analyzed homologs of UMAMIT29. (frontiersin.org)
Based on distance-based protein folding tools, we predict the substrate-transporting cavity of UMAMIT29. (frontiersin.org)
Based on our model, we proposed a substrate-transporting cavity of UMAMIT29 defined by helix I to IV and VI-IX and selected 51 residues as putative substrate binding sites based on solvent accessibility ( Figure 1 ). (frontiersin.org)
An example of enzymes that bind a single substrate and release multiple products are proteases , which cleave one protein substrate into two polypeptide products. (wikipedia.org)
There might be some difficult calculation to make about this, but anything like that anything like that is surely not expected in a question where they do not even tell you the concentration of your substrate protein! (physicsforums.com)

The protein is shown as a ribbon diagram , with alpha helices in red, beta sheathes in yellow and loops in blue. (wikipedia.org)
Proteins S4 and S7, which initiate assembly of the 16S rRNA, are located at junctions of five and four RNA helices, respectively. (embl.de)

While the crucial activities of decoding and peptide transfer are RNA based, proteins play an active role in functions that may have evolved to streamline the process of protein synthesis. (embl.de)
We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
Each serotype of BoNT hydrolyzes their protein substrates at different peptide bonds. (cdc.gov)
Disulphide bonds forming loops of amino acids of the peptide backbone have a major impact on internal dynamics that can be interpreted with a reduced set of Zimm modes. (lu.se)

For example, phosphorylation mainly occurs on a subset of three types of amino acids, including serine (S), threonine (T) and tyrosine (Y). Methylation is predominantly found on lysine (K) and arginine (R) residues. (deepdyve.com)
In some proteins, the side chain of serine appears to undergo ionization. (quizlet.com)

The functional amino acid residues in these proteins have been previously identified using in vitro and in silico experimentation in different biomineralization systems. (elsevierpure.com)
Intrinsic brain RAS is an enzyme-neuropeptide system having functional components (angiotensinogen, peptidases, angiotensin, and specific receptor proteins) with important biological and neurobiological activities in the brain. (hindawi.com)
To increase the utilization of current computational resources, we 﫿rst provide an overview of computational prediction of amino acid variations that influence protein PTMs and their functional analysis. (deepdyve.com)
Rosetta has full support for the 19 D-amino acids that are mirror images of the 19 canonical L-amino acids, and these are fully functional with the talaris2013 , talaris2014 , and beta_nov15 scoring functions. (rosettacommons.org)
These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)

c ) The 5.8 Å density map and superimposed 2.7 Å structure of the Torpedo receptor (PDB entry 6uwz ) obtained from detergent-solubilized protein complexed with α -bungarotoxin. (iucr.org)
IGF-1 then acts as a ligand and can interact with Insulin Receptor protein and Insulin-like Growth Factor Binding Protein . (proteopedia.org)
Insulin-like Growth Factor 1 Receptor ( IGF-1R ) is a transmembrane protein receptor. (proteopedia.org)
These toxins inhibit the release of neurotransmitters at the neuromuscular junction by cleaving soluble NSF attachment protein receptor (SNARE) complex proteins in nerve cells, which prevents the fusion of neurotransmitter containing vesicles to cell membranes 2 . (cdc.gov)

In this study, we have demonstrated a defect in the specific region or substituted acidic amino acid residues in the Mms6 protein for observing their effect on magnetite biomineralization in vivo. (elsevierpure.com)
Thus, these consecutive acidic amino acid residues were found to be core residues regulating magnetite crystal morphology. (elsevierpure.com)

The mature protein, which also includes a C-terminal truncation, contains 78 amino acid residues (92 amino acid residues for the murine homolog, without C-terminal truncation). (peprotech.com)

In our body, Metallochaperone is a family of soluble metal binding proteins involved in the intracellular transport of specific metal ions and their transfer to the appropriate metalloprotein precursor. (wellnessadvantage.com)
Explain why amino acids are insoluble in diethyl ether but N-acetyl amino acids are soluble. (quizlet.com)

Protein posttranslational modifications (PTMs) play key roles in a variety of protein activities and cellular processes. (hindawi.com)
Different PTMs show distinct impacts on protein functions, and normal protein activities are consequences of all kinds of PTMs working together. (hindawi.com)
With the development of high throughput technologies such as tandem mass spectrometry (MS/MS) and next generation sequencing, more and more nonsynonymous single-nucleotide variations (nsSNVs) that cause variation of amino acids have been identified, some of which result in the damage of PTMs. (hindawi.com)
More than 200 different types of protein posttranslational modifications (PTMs) have been detected. (hindawi.com)
PTMs are involved in many protein activities and cellular processes, such as protein folding, stability, conformation, and some significant regulatory mechanisms [ 1 ]. (hindawi.com)
The development of better methods for mutation analysis-related protein PTMs will help to facilitate the development of personalized precision medicine. (deepdyve.com)
posttranslational modifications, amino acid variations, computational mutation analysis, protein PTM predictor, network biology Introduction Protein PTMs are biochemical alterations of amino acids that change the physicochemical properties of target proteins, leading to structural changes and therefore regulating protein-protein interactions and cellular signal transduction in developmental and cancer pathways [1]. (deepdyve.com)
Almost all the amino acids undergo the process of PTMs, except leucine (L), isoleucine (I), valine (V), alanine (A) and phenylalanine (F) [2]. (deepdyve.com)
PTMs are specific to types of amino acid residues. (deepdyve.com)
Most PTMs are catalyzed by highly specific protein modifying enzymes, which have some specific recognition motif. (deepdyve.com)
7] analyzed amino acid variations of 15 different PTMs and indicated that about 4.5% of amino acid variations may affect protein function through disruption of PTMs, and the mutation of 238 PTMs sites in human proteins was causative of disease. (deepdyve.com)
In this regard, comprehensive studies of the impact of amino acid variation on protein PTMs will be helpful for further understanding of how genetic polymorphisms are involved in regulating biological and pathological processes and providing instructive information for drug development of various related diseases. (deepdyve.com)

In protein science , Molecular Chaperones , in our body's cells, are a family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. (wellnessadvantage.com)
In our body, Molecular Chaperones are able to inhibit the aggregation of partially denatured proteins and refold them. (wellnessadvantage.com)
ApoJ (clusterin) , also known as testosterone repressed prostate message-2, sulfated glycoprotien-2, and Sp-40 and CLU, functions as a secreted molecular chaperone protein , which may have either an intracellular or extracellular function. (wellnessadvantage.com)
AGT is a high molecular weight molecule (49.548), made up of 453 amino acids (aa) synthesized in the liver. (hindawi.com)
Extensive evidence has revealed that the core clock machinery involves "clock genes" and "clock proteins" functioning as molecular cogs. (bioone.org)
Take a very crude value of 100 for the average amino acid molecular mass, then that is an approximately 1M solution of amino acid. (physicsforums.com)
For instance, because the body's molecular machinery won't recognize them, MI DNAs, MI RNAs, and MI proteins resist enzymatic degradation and can largely avoid triggering an immune response, making them attractive drug candidates. (acs.org)
One of the key molecular events in skin sensitization is protein haptenation, i.e. the chemical modification of self-skin protein(s) thus forming macromolecular immunogens. (cdc.gov)
The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)
Our findings provide clues to the molecular mechanism of skin barrier formation by protein-bound ceramides. (bvsalud.org)

Despite their seemingly limitless diversity, all forms of life rely on biomolecules of a certain "handedness"-specifically L -amino acids and D -nucleic acids. (acs.org)

In the present study, we explored the structural aspect of HomA and HomB proteins using various computational, biophysical and small-angle X-ray scattering (SAXS) techniques. (nature.com)
Our study provides essential structural information of unexplored proteins of the Hom family that can help in a better understanding of H. pylori pathogenesis. (nature.com)
To investigate the relation between protein stability and the predicted stabilities of individual secondary structural elements, residue Pro86 in an alpha-helix in phage T4 lysozyme was replaced by ten different amino acids. (rcsb.org)
These observations demonstrate that the water relaxation dispersion in protein solutions is essentially due to a few structural water molecules buried within the protein matrix, but exchanging rapidly with the external water. (lu.se)
A general property of disordered proteins is their structural expansion that results in a high macromolecular flexibility. (lu.se)

Explain why ionization would be facilitated by the presence of an aspartate residue nearby. (quizlet.com)

There are some special cases in which posttranslational enzymatic modification of certain bacterial cell wall proteins produces a few D-residues. (rosettacommons.org)

The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. (thermofisher.com)
The proteins are named in accordance with the subunit of the ribosome which they belong to - the small (S1 to S31) and the large (L1 to L44). (embl.de)
Many ribosomal proteins, particularly those of the large subunit, are composed of a globular, surfaced-exposed domain with long finger-like projections that extend into the rRNA core to stabilise its structure. (embl.de)
In the large subunit, about 1/3 of the 23S rRNA nucleotides are at least in van der Waal's contact with protein, and L22 interacts with all six domains of the 23S rRNA. (embl.de)

DSB sites are preferentially located within chromatin loops, while several proteins that are required for DSB formation (Rec114, Mei4, and Mer2) localize on the chromosome axis (Blat et al. (springer.com)

For the most part, D-amino acids are not incorporated into natural proteins, since the ribosome translates D-residues very inefficiently. (rosettacommons.org)
About 2/3 of the mass of the ribosome consists of RNA and 1/3 of protein. (embl.de)

For instance, the SwissVariant database (http://swissvar.expasy.org/) contained 76 613 variants in 20 244 human proteins on 10 January 2018. (deepdyve.com)

New technologies for purifying membrane-bound protein complexes in combination with cryo-electron microscopy (EM) have recently allowed the exploration of such complexes under near-native conditions. (researchgate.net)
Protein complexes, which are formed as a result of these interactions, consist of two or more components that associate along specific pathways - protein association pathways. (lu.se)

Conventionally, the mirror-image versions of the 19 canonical chiral amino acids are called by the same names (D-tyrosine is the mirror image of L-tyrosine, for example). (rosettacommons.org)
There are two special cases in which we have to be careful about definitions: the two canonical amino acids that also have a chiral center in the side-chain (L-threonine and L-isoleucine) have this chiral center reversed in their D-forms, as well, making D-threonine the true mirror image of L-threonine and D-isoleucine the true mirror image of L-isoleucine. (rosettacommons.org)
D-amino acid residues are considered "non-canonical" by Rosetta. (rosettacommons.org)
rama and rama_prepro (Completely ignores noncanonicals, unless the BACKBONE_AA line is included in the params file, and this line currently only allows canonical L-amino acids to be specified. (rosettacommons.org)

2021 ). The human DBNDD1 isoforms 1 (UniProtKB: Q9H9R9-1) and 2 (UniProtKB: Q9H9R9-2) differ only in the N-terminal region where 20 amino acids are additional in isoform 2. (springer.com)
Unlike other dysbindin family proteins, DBNDD1 isoforms are probably non-classical secretory proteins (Talbot et al. (springer.com)
The coding RNA of this protein creates 4 isoforms differing from each other due to RNA splicing. (proteopedia.org)

Our chemical-shift based secondary structure analysis reveals the human DBNDD1 as an intrinsically disordered protein. (springer.com)
2021 ), https://pfam.xfam.org/ ] predicts human DBNDD1 mainly as an intrinsically disordered protein (IDP) and also the recently released AlphaFold database (Jumper et al. (springer.com)

The product can be reconstituted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. (bdbiosciences.com)
Protein performs its biological functions by interacting with other proteins. (lu.se)

Catalytically inactive proteins have been converted into modestly catalytic ones for two different reactions, but the observed enhancements of rate 6 , 7 were only about a millionth of those produced by naturally occurring enzymes. (nature.com)
The main difference between ribozymes and enzymes is that RNA catalysts are composed of nucleotides, whereas enzymes are composed of amino acids. (wikipedia.org)
Ting Zhu of Tsinghua University hopes to fill that gap by creating not only mirror-image (MI) DNA strands but also MI enzymes that can copy this unnatural DNA, transcribe it into MI RNA, and, eventually, translate the MI RNA into MI protein. (acs.org)
Most selenoproteins are enzymes with the selenocysteine residues being responsible for their catalytic functions. (bvsalud.org)

Neutron Spin-Echo spectroscopy (NSE) on the other hand, offers the highest energy resolution in the field of neutron spectroscopy and allows the study of slow collective motions in proteins up to several hundred nanoseconds and in the nanometer length-scale. (lu.se)

This leads to the incorporation of amino acids into the growing polypeptide chain in accordance with the genetic information. (embl.de)
Ribosomal protein L31e, which is present in archaea and eukaryotes, binds the 23S rRNA and is one of six protein components encircling the polypeptide exit tunnel. (embl.de)
A polypeptide contains more than ten amino acids, while a protein has more than 100 amino acids. (quizlet.com)

A globular protein containing approximately 2. (edurev.in)
Resonance Group, Chemical of the globular proteins bovine pancreatic trypsin inhibitor (BPTI) and Center, Lund University ubiquitin in aqueous solution. (lu.se)
This difference was ascribed to the abscence, in ubiquitin, of highly ordered internal water molecules, which are known to be present in BPTI and in most other globular proteins. (lu.se)

methylation at certain residues of histones can regulate gene expression [ 4 ], and glycosylation is responsible for targeting substrates and changing protein half-life [ 2 ]. (hindawi.com)

and even the role of particular amino acid residues in the mechanism. (wikipedia.org)
The exact residues interacting with one another are not known accuraretly but the docking mechanism is the same as for IGFBP. (proteopedia.org)

For a protein made of 100 amino acids, there are about 10 130 possible sequences, each of which can adopt many different conformations. (nature.com)
Their amino acid sequences suggest that they are mainly cytosolic or nuclear proteins partly associating with membranes (Talbot et al. (springer.com)
We performed a Basic Local Alignment Search Tool (BLAST) analysis to identify regions of local similarity between the human DBNDD1 and protein sequences from other species (Fig. 1 ). (springer.com)
2019 ) was used, https://www.ebi.ac.uk/Tools/msa/clustalo/ ] of human DBNDD1 and similar protein sequences found by a BLAST search in other selected species. (springer.com)

As reported earlier, the helical arrangement of the protein component of the vesicles facilitates image averaging and enables us to determine how cholesterol and phospho-lipid molecules are distributed in the surrounding matrix, using headgroup size as a means to discriminate between the two kinds of lipid. (iucr.org)
2022 ) predicts human DBNDD1 - with a short stretch of helical propensity between residues L77 and S95 - entirely as an IDP. (springer.com)
This insensitivity of stability to the amino acid at position 86 is not simply explained by statistical and thermodynamic criteria for helical propensity. (rcsb.org)

Transport activity assays of mutant variants with a single point amino acid substitution in each site UMAMIT29 suggest that five of these residues are critical for glucosinolate transport. (frontiersin.org)

Hom (Helicobacter outer membrane) family of OMPs in H. pylori consists of four members (HomA, B, C and D). In the H. pylori genome jhp 0870 open-reading frame (ORF) that codes for HomB outer membrane protein is associated with many stomach diseases and is a novel co-marker for peptic ulcer disease (PUD) 9 , 10 . (nature.com)
It consists of 14 separate exons spanning approximately 7.7kb of genomic DNA, and encodes the 672 amino acid protein SGLT2. (medscape.com)

Outer membrane proteins (OMPs) of H. pylori play a crucial role in the host-pathogen interaction, virulence and pathogenesis. (nature.com)
PRDM9 is a member of the PRDM family of transcription regulators, but unlike other family members, it contains a Krüppel-associated box (KRAB)-related domain that is predicted to be a potential protein interaction domain. (springer.com)
In S. cerevisiae , this interaction is at least in part provided by Spp1 that directly interacts with both methylated H3K4 near DSB sites and the axis-localized protein Mer2 (Acquaviva et al. (springer.com)
protein interaction has still not emerged. (lu.se)

Therefore, the various concentration of the insulin proteins regulates the cell activity in different context, for instance in excess of glucose or lack of Growth Hormone. (proteopedia.org)
However, the concentration of total proteins was higher in CG (201.2 ± 100 mg/dl) than TG (155.0 ± 95 mg/dl). (bvsalud.org)

Cross-talk of the MAPK signaling pathway with other proteins and signaling pathways have a crucial impact on clinical outcomes of targeted therapies and plays important role during development of drug resistance in cancers. (mdpi.com)
The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)

The reason is α-amino acids are weak bases (pK a of their -NH 3 + group about 9.3) while the carboxyl groups are rather strong acids with average pK a around 2.2. (physicsforums.com)
So calculate firstly what is the pH of 1M amino acid solution if all the side chains are neutral and the carboxyl pK a 's an average 2.2. (physicsforums.com)
B. It attaches the carboxyl group of one amino acid to the carboxyl group of a second amino acid. (quizlet.com)

The simple way to determine whether an alpha-amino acid is a D-amino acid or an L-amino acid is to imagine holding the backbone amide in your left hand and the backbone carbonyl in your right hand, with the alpha carbon pointing out in front of you (as though you were holding a dowsing rod). (rosettacommons.org)
Here, we present the backbone and side chain nuclear magnetic resonance assignments for the human DBNDD1 protein. (springer.com)

Vitamin K is an essential cofactor for γ-glutamyl carboxylase enzymatic activity that catalyses the γ-carboxylation of specific glutamic acid residues in a subclass of proteins. (medscape.com)

When each of the 11 residues of UMAMIT29 was individually mutated into the corresponding residue in UMAMIT32, five mutant variants (UMAMIT29#V27F, UMAMIT29#M86V, UMAMIT29#L109V, UMAMIT29#Q263S, and UMAMIT29#T267Y) reduced glucosinolate transport activity over 75% compared to wild-type UMAMIT29. (frontiersin.org)

b. some codons specify more than one amino acid. (quizlet.com)
c. some codons do not specify any amino acid. (quizlet.com)
d. some amino acids do not have codons. (quizlet.com)

The dysbindin domain-containing protein 1 (DBNDD1) is a conserved protein among higher eukaryotes whose structure and function are poorly investigated so far. (springer.com)
The dysbindin (dystrobrevin-binding protein) protein family is a group of evolutionarily related proteins of moderate size (Mw 13-45 kDa) in higher Eukaryotes. (springer.com)

Recently, members of the Usually Multiple Amino acids Move In and Out Transporter (UMAMIT) family were shown to be essential for facilitating transport of seed-bound glucosinolates from site of synthesis within the reproductive organ to seeds. (frontiersin.org)

third, the protein should directly interact with at least one other member of the complex. (wellnessadvocate.com)
In addition, we identified proteins that interact with the KRAB domain of PRDM9 in yeast two-hybrid assay screens, particularly CXXC1, a member of the COMPASS complex. (springer.com)
Most of the proteins interact with multiple RNA elements, often from different domains. (embl.de)

Botulinum neurotoxins (BoNTs) are bacterial proteins that cause the life-threatening disease botulism 1 . (cdc.gov)

HomB protein contributes to the colonization and persistence of H. pylori , and the presence of homB genes affects the number of bacteria adhering to the host cells. (nature.com)
The tub gene is a member of a family of tubby-like genes (TULPs) that encode proteins of unknown function. (nih.gov)

Ribosomes are the particles that catalyse mRNA-directed protein synthesis in all organisms. (embl.de)

Trypsin is an enzyme that catalyses the breakdown of proteins into amino acids. (physicsforums.com)

Nature rarely uses the mirror-image versions of these molecules, D -amino acids and L -nucleic acids. (acs.org)
The water molecules residing at the surface of the two proteins were found to be highly mobile, with an average rotational correlation time of approximately 20 picoseconds. (lu.se)

The light chain is a zinc-metalloprotease which is responsible for the cleavage of three SNARE complex proteins including SNAP-25 (Synaptosome-associated protein of 25 kDa), synaptobrevin-2 (also termed VAMP-2) and syntaxin. (cdc.gov)

Insulin-like Growth Factor ( IGF-1 ) is a member of the insulin protein family. (proteopedia.org)
Insulin is an essential endocrine protein. (proteopedia.org)
Once IGF1 is bound to Insulin-like Growth Binding Protein (IGFBP), IGF-1 cannot be linked to IGF1R any longer. (proteopedia.org)

The reaction at neutral pH produces or consumes consumed no net protons gives essentially no pH change.There might be a secondary effect due to a few amino acid side chain groups by themselves having a different pK than those same groups when they are part of the intact protein. (physicsforums.com)

Extracellular signal-regulated kinase (ERK) is a part of the mitogen-activated protein kinase (MAPK) signaling pathway which allows the transduction of various cellular signals to final effectors and regulation of elementary cellular processes. (mdpi.com)

This page has information on how to activate these residue types, as well as on working with other D-amino acids with noncanonical side chains. (rosettacommons.org)

The amino acid variation S326C of human OGG1 disrupts Ser-326 phosphorylation site and affects susceptibility to a variety of cancers [9]. (deepdyve.com)

IGF-1 is a peptidic hormone of 69 amino acids which is secreted by the liver. (proteopedia.org)

The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)
In addition, the effectiveness of antitoxin treatment may vary depending on circulating toxin levels, so that rapid identification and quantification of BoNT proteins in clinical specimens and food is desirable for optimization of treatment. (cdc.gov)

Protein-bound ceramides, specialized ceramides covalently bound to corneocyte surface proteins, are essential for skin permeability barrier function. (bvsalud.org)
Here, we found that epoxy-enone (EE) ceramides, precursors of protein-bound ceramides, as well as their synthetic analog, formed stable conjugates only with Cys among nucleophilic amino acids. (bvsalud.org)
EE ceramides were reversibly released from protein-bound ceramides via sulfoxide elimination. (bvsalud.org)
We found that protein-bound ceramides with reversible release properties accounted for approximately 60% of total protein-bound ceramides, indicating that Cys-bound EE ceramides are the predominant protein-bound ceramides. (bvsalud.org)
In QENS experiments of protein solutions hydrogens act as reporters for the motions of methyl groups or amino acids to which they are bound. (lu.se)

Analysis of the gene deletion mutants and transformants of Magnetospirillum magneticum AMB-1 expressing partially truncated Mms6 protein revealed that deletions in the N-terminal or C-terminal regions disrupted proper protein localization to the magnetite surface, resulting in a change in the crystal morphology. (elsevierpure.com)

abstract = "Living organisms produce finely tuned biomineral architectures with the aid of biomineral-associated proteins. (elsevierpure.com)

With these precedents, you might think that designing catalytic proteins should be straightforward, but success has been limited. (nature.com)
An essential step in demonstrating the success of a designer enzyme, therefore, is the determination of a high-resolution crystal structure for the protein, to verify that the designed catalytic features are present. (nature.com)

In humans and mice, hotspots are specified by PRDM9, a meiosis-specific PRDM family protein (Baudat et al. (springer.com)
FYN is a member of the protein-tyrosine kinase oncogene family. (thermofisher.com)

Amino acid residues 220-231 of the wild-type ApoA-I are required for lipid efflux in vitro and high density lipoprotein formation in vivo. (medlineplus.gov)

Note that residue numbers are given as in the PDB file, not the "order number", but the fragments will be renumbered in consecutive order. (lu.se)

For both proteins, the oxygen-17 relaxation depended only very weakly on pD, showing that ionic residues do not perturb hydration water dynamics more than other surface residues. (lu.se)

Anatomy1

Organisms1

Chemicals and Drugs7

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes17

Information Science4

Peptides2

Sequence14

Cysteine residues present2

Approximately 2004

Substitutions3

Functionally important2

Mutations7

Recombinant6

Substrate5

Helices2

Peptide6

Serine2

Functional5

Receptor4

Acidic2

Murine1

Soluble2

PTMs12

Molecular10

Biomolecules1

Structural5

Aspartate1

Posttranslational1

Subunit4

Loops1

Ribosome2

Human Proteins1

Complexes2

Canonical4

Isoforms3

Intrinsically disordered protein2

Biological2

Enzymes4

Spectroscopy1

Polypeptide3

Globular3

Glycosylation1

Mechanism2

Sequences4

Helical3

Substitution1

Consists2

Interaction4

Concentration2

Pathways2

Carboxyl3

BACKBONE2

Enzymatic1

Variants1

Codons3

Eukaryotes2

Transporter1

Interact3

Bacterial1

Genes2

Synthesis1

Catalyses1

Molecules2

SNARE1

Insulin3

Secondary1

Transduction1

Side chains1

Phosphorylation1

Secreted by the liver1

Identification2

Bound5

Partially1

Abstract1

Catalytic2

Family2

Vitro1

Consecutive1

Water Dynamics1