Residues protein domain carrier proteins. Medical search. Frequent questions

The enzyme is capable of transferring the 4'-phosphopantetheine moiety of coenzyme A to a conserved serine residue in both the acyl carrier protein domain of the human cytosolic multifunctional fatty acid synthase and the acyl carrier protein associated independently with human mitochondria. (rhea-db.org)
The human 4'-phosphopantetheine transferase is also capable of phosphopantetheinylation of peptidyl carrier and acyl carrier proteins from prokaryotes. (rhea-db.org)
The α-subunits of the oxaloacetate and methylmalonyl-CoA decarboxylases are homologous to many biotin-containing enzymes including (1) pyruvate carboxylases, (2) homocitrate synthases, (3) biotin carboxyl carrier proteins, (4) isopropylmalate synthases and (5) acyl-CoA carboxylase. (tcdb.org)
Most members of the MIT family are between 300 and 400 amino acyl residues in length and possess two (or three) putative transmembrane α-helical spanners (TMSs). (tcdb.org)
The yeast metal resistance proteins, which are 850-900 amino acyl residues in length, also exhibit two or three putative TMSs. (tcdb.org)
The CorA proteins of E. coli and S. typhimurium are each 316 amino acyl residues in length. (tcdb.org)
The animal AE proteins consist of homodimeric complexes of integral membrane proteins that vary in size from about 900 amino acyl residues to about 1250 residues. (tcdb.org)

Our findings suggest that macro domain tags can be employed in recombinant protein expression in E. coli, and in carrier-driven crystallization. (rcsb.org)
This research delves into the intricacies of thyroglobulin human recombinant protein, exploring its biochemical properties, physiological significance, and its crucial applications in both clinical and research settings. (prospecbio.com)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
The 384 amino acid recombinant protein has a predicted molecular mass of approximately 42.7 kD. (biolegend.com)
The recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. (prosci-inc.com)
When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. (prosci-inc.com)

Comprising a series of tyrosine residues, thyroglobulin serves as the scaffold upon which thyroid hormones are assembled. (prospecbio.com)
Within its structure lie tyrosine residues crucial for iodine incorporation, a process fundamental for thyroid hormone synthesis. (prospecbio.com)
CD112R gene encodes a single transmembrane protein consisting of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain which contains two tyrosine residues, one within an ITIM-like motif. (biolegend.com)

HABP1 assembles into a doughnut shaped trimer, with negatively charged residues asymmetrically distributed on one face lining the channel of the complex (4). (rndsystems.com)
Two negatively charged helices in the cytoplasmic domain extend back towards the membrane on the outside of the funnel and abut the ring of positive charge. (tcdb.org)

We have structurally characterized the final peptidyl carrier protein domain of the teicoplanin non-ribosomal peptide synthetase machinery: this domain is believed to mediate the interactions with tailoring Oxy enzymes in addition to its function as a shuttle for intermediates between multiple non-ribosomal peptide synthetase domains. (rcsb.org)

3. Chimerical peptides according to the claim 1, characterized by containing a segment inhibitor of the activation of the NS3pro protease, this segment is a peptide able to bind to NS3pro and contact at least one residue comprised in the region Gly21-Lys28 of the N-terminal domain of the NS3pro protein from DV2 or structurally equivalent residues of the NS3pro protein from other Flavivirus. (patentsencyclopedia.com)
5. Chimerical peptides according to the claim 1, where the mentioned segment inhibitor of the activation of the NS3pro protease contact at least one residue comprised in the region Glu32-Thr38 of the N-terminal domain of the NS3pro protein from HCV or structurally equivalent residues of the NS3pro protein from other Hepacivirus. (patentsencyclopedia.com)
6. Chimerical peptides according to the claim 1, characterized by containing a segment inhibitor of the activation of the NS3pro protease which comprise the region Asp50-Glu62 of the NS2B protein from DV2 or the structurally equivalent segment of the protein NS2B from other Flavivirus, or an amino acid sequence showing a sequence similarity higher of equal to the 80% identity. (patentsencyclopedia.com)
8. Chimerical peptides according to the claim 1, characterized by containing a segment inhibitor of the activation of the NS3pro protease which comprise the region Thr20-Leu31 of the NS4A protein from HCV or the structurally equivalent segment of the protein NS4A from other Hepacivirus, or an amino acid sequence showing a sequence similarity higher of equal to the 80% identity. (patentsencyclopedia.com)
Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
Cyclic peptides are poised to target historically difficult to drug intracellular protein-protein interactions, however, their general cell impermeability poses a challenge for characterizing function. (bvsalud.org)
Water 2H and 17O spin relaxation is used to study water dynamics in the hydration layers of two small peptides, two globular proteins and in living cells of two microorganisms. (lu.se)
This distribution comprises a small fraction of protein-specific hydration sites, where water rotation is strongly retarded, and a dominant fraction of generic hydration sites, where water rotation is as fast as in the hydration shells of small peptides. (lu.se)

Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor (10 - 16). (rndsystems.com)

The β-subunits possess 9 transmembrane α-helical spanners (TMSs), and the protein may dip into the membrane twice between TMSs III and IV. (tcdb.org)
The greatest degree of conservation between homologues is found in TMSs 1 and 2 of the Thermotoga maritima protein. (tcdb.org)
Hydropathy analysis had predicted two transmembrane α-helical spanners (TMSs) in the C-terminal regions of these proteins. (tcdb.org)

Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae. (ymdb.ca)

Given the tight relation between protein structure and function, we present a set of methods to analyze protein topology, implemented in the VLDP program, relying on Laguerre space partitions built from series of molecular dynamics snapshots. (nature.com)
Topology is a prominent aspect of both morphology and functioning of proteins. (nature.com)
In this respect, and also with respect to topology, MIT family members resemble channel proteins. (tcdb.org)
All AE proteins are hypothesized to share a similar topology in the cell membrane. (tcdb.org)

2006 ) who identified a soluble oligomeric N-terminal domain of the E. coli CorA that appeared to be tetrameric and to bind its substrates with the same affinity of native CorA ( Wang et al . (tcdb.org)

5 4 Succinate functions as a competitive inhibitor for prolyl hydroxylase domain (PHD) proteins that are central to degradation of hypoxia-inducible factor (HIF)-1α subunit. (haematologica.org)
The enzyme transfers CO 2 from glutaconyl-CoA to a biotin carrier protein (the γ-subunit) that is subsequently decarboxylated by the carboxybiotin decarboxylation site within the Na + pumping beta subunit (Gcdβ). (tcdb.org)
DT molecule is composed of two subunits: subunit A and subunit B. The A-subunit of DT is represented by a single domain - the catalytic or C-domain which modifies eEF-2. (org.ua)
B-subunit is composed of two domains: receptor domain or R-domain, which is responsible for binding to cell DT receptor, and transmembrane or T-domain, which is involved in the process of C-domain transfer in the cytosol across endosomal membrane. (org.ua)
DOK3 interacted with CARD11 and decreased its phosphorylation in T cells by recruiting the catalytic subunit of protein phosphatase 4, thereby dampening downstream signaling. (bvsalud.org)

This group is composed of Mycobacterium tuberculosis putative mycofactocin radical SAM maturase MftC and similar proteins. (nih.gov)

Search proteins in UniProtKB for this molecule. (rhea-db.org)
The molecule of this receptor is composed of four functional portions: heparin-binding, EGF-like, transmembrane and cytoplasmic domains. (org.ua)

In the majority of cases, Rf genes produce proteins that act directly on the CMS conferring mitochondrial transcripts by binding them specifically and promoting processing events. (frontiersin.org)
These unusual ORFs are maternally inherited, and effectively translated into novel mitochondrial proteins, with the resulting failure to produce functional pollen as the sole observed phenotype ( Chen and Liu, 2014 ). (frontiersin.org)
In the majority of cases, Rf genes produce proteins that bind specifically to the CMS conferring transcripts in the mitochondria and promote processing events leading to a strong reduction in the production of mitochondrial CMS-inducing proteins (reviewed in Chen and Liu, 2014 ). (frontiersin.org)

An apparent Mg 2+ ion was bound between monomers at a conserved site in the cytoplasmic domain suggesting a mechanism to link gating of the pore to the intracellular concentration of Mg 2+ . (tcdb.org)
The intracellular funnel domain of CorA constitutes an allosteric regulatory module that can be engineered to promote an activated or closed state ( Payandeh et al . (tcdb.org)
2007). These proteins are important for the regulation of intracellular pH (pH i ) and play crucial roles in the epithelial absorption of HCO 3 - (e.g., in the renal proximal tubule) and secretion of HCO 3 - (e.g., in the pancreatic duct). (tcdb.org)

mutation of a centrally located tyrosine residue dramatically lowers the affinity of NEPH1 for podocin. (nih.gov)
The p.Asn173His mutation affects a residue in the forkhead domain that is 100% conserved among vertebrate orthologs and is predicted to participate in protein-protein interactions. (molvis.org)
Moreover, we have identified several conserved residues in the CSS anti-sigma factor family of which mutation leads to constitutive activation of their cognate σECF. (vu.nl)

The gene is 160 kilobases in length and has 15 exons and 14 introns with specific structural and functional domains. (medscape.com)

The PPR protein family is consequently divided in subfamilies depending on the number and type of repeats present in their sequence as well as optional C-terminal domains. (frontiersin.org)

The cytoplasmic neck of the pore is surrounded, on the outside of the funnel, by a ring of highly conserved positively charged residues. (tcdb.org)

Cyanobacterial phycobilisome (PBS) pigment-protein complexes harvest light and transfer the energy to reaction centers. (osti.gov)

The crystal structure of the CorA homologue from Thermotoga maritima has been solved at 3.9 Å resolution for the full-length protein and at 1.85 Å resolution for the cytoplasmic domain ( Lunin et al . (tcdb.org)
The large cytoplasmic domain forms a funnel whose wide mouth points into the cell and whose walls are formed by five long helices that are extensions of the transmembrane helices. (tcdb.org)

The CusB protein is part of the CusCBA periplasmic Cu(I) efflux system in Gram-negative bacteria, and it was recently reported to play a key role in the functioning of the whole CusCBA system, in which conformational changes as well as the assembly/disassembly process control the opening of the transporter. (biorxiv.org)
We performed a random mutagenesis screen of the unique IutY protein of Pseudomonas putida, a protein that combines a cytosolic σECF domain and a periplasmic anti-sigma factor domain in a single polypeptide. (vu.nl)
Our experiments show that all IutY mutant proteins that contain periplasmic residues depend on RseP for activation. (vu.nl)
In contrast, Prc is only required for mutant variants with a periplasmic domain longer than 50 amino acids, which indicates that the periplasmic region of IutY is trimmed down to ~50 amino acids creating the RseP substrate. (vu.nl)
Highly conserved acidic residues found in the short periplasmic loop are not essential for CorA function or Mg 2+ selectivity but may be required for proper protein folding and stability. (tcdb.org)

These structures thus support the hypothesis that it is subtle rearrangements, rather than dramatic conformational changes, which govern carrier protein interactions and selectivity during non-ribosomal peptide synthesis. (rcsb.org)
The combination of such self-organizational phenomena with canonical intermolecular interactions is most likely to control the release of membrane proteins from the ER into the secretory pathway. (biologists.com)
This process depends on the general physico-chemical features of the cargo membrane protein and on the interactions of these features with the collective properties of the bilayer, instead of the one-to-one intermolecular interactions that exist between discrete signals and their receptors. (biologists.com)
Although the C-terminal domain comprises a small percentage of the total protein, this domain in some cases (i) has PSD-95/Discs Large/ZO-1-binding motifs that may be important for protein-protein interactions (e.g. (tcdb.org)

Results from comparative analysis of residue conservation, conformational mobility, and coevolutionary patterns for uracil-DNA glycosylase. (bahargroup.org)

This protein family is largely expanded in land plant genomes. (frontiersin.org)

Overexpression of the yeast proteins, Al R 1p and Mn R 2p, overcomes toxicity to aluminum and manganese, respectively. (tcdb.org)
The yeast proteins appear to exhibit broad specificity transporting a wide range of di- and trivalent metal cations. (tcdb.org)
Characterized protein members of the AE family are found in animals, plants and yeast. (tcdb.org)

Here, we combine in-vitro structural measurements, which used EPR spectroscopy and UV-Vis measurements, with cell experiments to explore the role of the various methionine residues in CusB. (biorxiv.org)
SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
Dynamics of p38 MAP kinase inferred from a structural ensemble using PCA is compared to intrinsic dynamics of the protein modeled using ANM. (bahargroup.org)
Structural domains and CCDDs are identified and outlined. (bahargroup.org)
This review critically focuses on opportunities to employ protein-graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. (mdpi.com)
Previously, we have designed genetic constructs that encode various recombinant derivatives of DT and proHB-EGF structural parts and domains. (org.ua)
Their N-terminal hydrophilic domains may interact with cytoskeletal proteins and therefore play a cell structural role. (tcdb.org)
tRNA thiolation factors Urm1?Uba4 represent the most ancestral known ubiquitin?like protein/E1 enzyme conjugation system, whose structural mechanisms have however remained elusive. (fnp.org.pl)

The 511 amino acid (aa) residue mature human NETO1 contains a 322 aa extracellular domain (ECD) with two CUB domains and one class A LDL-receptor segment, a 21 aa transmembrane domain, and a 168 aa cytosol domain (1). (bio-techne.com)
These receptors typically contain five subunits with the cysteine-loop occurring near an N-terminal extracellular domain. (bvsalud.org)

The channel is formed by an inner group of five helices and putatively gated by bulky hydrophobic residues. (tcdb.org)

Our study uses classical algorithms such as space partitions and Dijkstra paths search 9 , applied in an original and novel way by taking into account the full representation of a membrane protein including the solvent and the lipid bilayer (Fig. 1 ). (nature.com)
The FepA membrane protein is surrounded by a lipid bilayer separating the extracellular medium and the periplasm. (nature.com)
The formation of AG is set up in the cytoplasm on the decaprenyl phosphate (Dec-P) carrier lipid with formation from the linker device accompanied by the addition of Galand Araresidues (10,C12). (sicollaborative.org)
For membrane proteins, a third mechanism, based on the interaction of their transmembrane domain (TMD) with lipid microdomains, must also be considered. (biologists.com)
The exact molecular mechanism of C-domain transport across the lipid bilayer is unknown but it is known that T-domain is strongly involved in this process. (org.ua)

Sex hormone binding globulin (shbg) is a glycoprotein composed of 373 amino acid residues and three carbohydrate side chains. (jessilafree.com)
Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is a ubiquitous acidic glycoprotein that functions in spermatogenesis and as a receptor for proinflammatory molecules (1, 2). (rndsystems.com)
The natural receptor of DT, proHB-EGF is a single chain transmembrane glycoprotein of 208 amino acid residues. (org.ua)

Transport by partitioning is distinct from both bulk flow and receptor-mediated transport, because differently from bulk flow, cargo can be concentrated in transport carriers, yet the concentrating process does not depend on specific cargo receptors ( Fig. 1 and Box 1 ). (biologists.com)
A subfamily of ligand-gated ion channel receptors that share a characteristic loop which is formed by a disulfide bond between two CYSTEINE residues. (bvsalud.org)

The cytotoxic action of DT is mediated by catalytic transfer of ADP-ribosyl group from NAD + to the diphthamide residue (modified histidine residue) in eukaryotic translation elongation factor 2 (eEF-2). (org.ua)
An international team of researchers from the Max Planck Research Group at the Malopolska Centre of Biotechnology (MCB) deciphered the molecular mechanism of a sulfur carrier system that stands at the root of all eukaryotic Ubiquitin-conjugation systems. (fnp.org.pl)

We report now that NEPH1 belongs to a family of three closely related proteins that interact with the C-terminal domain of podocin. (nih.gov)

MftC is a radical S-adenosylmethionine (SAM) enzyme that may function to modify mycofactocin, a conserved polypeptide that might serve as an electron carrier. (nih.gov)
Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)

Thus, CNNM proteins, the vertebrate orthologues of CorB/C, also have Mg 2+ transport capacity. (tcdb.org)

Using solution state NMR, we have determined structures of this PCP domain in two states, the apo and the post-translationally modified holo state, both of which conform to a four-helix bundle assembly. (rcsb.org)
The structures exhibit the same general fold as the majority of known carrier protein structures, in spite of the complex biosynthetic role that PCP domains from the final non-ribosomal peptide synthetase module must play in glycopeptide antibiotic biosynthesis. (rcsb.org)
The structures were solved by molecular replacement, using isolated macro domain structures as search models. (rcsb.org)
Outward-facing (OF) and inward-facing (IF) structures of Glt Ph show a large displacement of the core domains. (bahargroup.org)

The team obtained the first three-dimensional atomic model of the E1-like sulfurtransferase, Uba4, with its bound substrate, the Ubiquitin-like protein (UBL), Urm1. (fnp.org.pl)

More knowledge of the underlying mechanism is needed to attain a full understanding of CusB functioning, which is associated with targeting specific and crucial residues in CusB. (biorxiv.org)
Our results highlight the participation of two of these proteins in the phosphotransferase reaction leading to the ligation of AG and PG, a crucial step in the assembly of the entire complex cell wall of H37Rv yielded three candidates: Rv0822c, Rv3267 (herein renamed CpsA1), and Rv3484 (herein renamed CpsA2). (sicollaborative.org)

Cell surface HABP1 binds a wide range of extracellular molecules, including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 6 - 9). (rndsystems.com)

GPR91 is a G protein-coupled cell surface receptor for extracellular succinate (Sucnr1). (haematologica.org)
CD112R was initially named PVRIG for the homology observed between its second exon and the variable immunoglobulin domain of the polio virus receptor (PVR/CD155) and polio virus receptor-like (PVRL) genes. (biolegend.com)
The scaffolding protein CARD11 is a critical mediator of antigen receptor signaling in lymphocytes. (bvsalud.org)
Neuropilin and tolloid-like protein 1 (NETO1), also known as Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 (BTCL1), is a type I transmembrane protein that is expressed in the brain and retina (1). (bio-techne.com)

The principal steroid binding proteins in plasma are albumin, corticosteroidbinding globulin (cbg, transcortin) sex-hormone binding globulin (shbg) which is. (jessilafree.com)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)

Accumulation of succinate associates with succinylation, i.e. addition of succinyl group to a lysine residue of a protein. (haematologica.org)
Succinate further inhibits several dioxygenases involved in epigenetic regulation like ten-eleven translocation methylcytosine dioxygenase (TET) and jumonji C domain-containing histone lysine demethylases (JMJD3). (haematologica.org)
The N-terminal domain binds the glutaconyl-CoA, and the C-terminal domain binds the biotinyl lysine moiety. (tcdb.org)

The three proteins share between 21 and 29% identity (37C50% similarity) with LCP proteins from (MsrR, SA0908, and SA2103), (CpsA2), and (TagT, TagU, and TagV) (Fig. 2maps to an AG biosynthetic gene cluster adjacent to ((genus and other AG-producing Actinobacteria, including species, and is a pseudogene in LCP protein candidates. (sicollaborative.org)

In case of proteins for which it is possible to identify the secondary structure, symbols are given in the Stride classification (e.g. (edu.pl)

When Recombinant Human NETO1 His-tag Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human APP695 +1 Fc Chimera binds with an ED 50 of 0.200-1.20 μg/mL. (bio-techne.com)

1 Similarly, succinate may derive from the γ-aminobutyric acid shunt pathway that correlates with levels of expression of the γ-aminobutyric acid transporters solute carrier family 6 members 12 and 13 (SLC6A12, SLC6A13). (haematologica.org)
As noted above, the AE or solute carrier 4 (SLC4) family includes 10 members (SLC4A1-5, SLC4A7-11), which are expressed in multiple tissues in the human body. (tcdb.org)

As a test-case, the membrane protein FepA is investigated in its full environment, yielding a more precise description of the protein surface. (nature.com)

In order for a cell to respond to changes in internal and external environmental factors, a broad range of protein co- and post-translational modifications have evolved to expand upon the relatively static properties encoded in protein side-chains. (biologists.com)

Characterization of the plasma sex steroid-binding protein. (jessilafree.com)

PPR proteins represent the most frequent protein class among identified Rfs and they exhibit ideal characteristics to evolve into restorer of fertility when the mechanism of restoration implies a post-transcriptional action. (frontiersin.org)
In this report, we demonstrate an alternate mechanism of JNK-mediated cell death involving the anti-apoptotic protein human apurinic/apyrimidinic endonuclease 1 (APE1). (bvsalud.org)

binding) protein 2/ steroid hormones have different affinity for binding. (jessilafree.com)

Perturbation of these specific modification pathways results in protein homeostasis defects and has been linked to neurological disorders and cancer in humans. (fnp.org.pl)
As this feature appears to be conserved in all other E1 and E1-like activating domains, the observed covalent E1-UBL self-conjugation reaction likely represents the evolutionary ancestor of most UBL pathways. (fnp.org.pl)

Use of the DNA polymerase chain reaction for homology probing: isolation of partial cDNA or genomic clones encoding the iron-sulfur protein of succinate dehydrogenase from several species. (ymdb.ca)

Anatomy9

Organisms14

Chemicals and Drugs102

Analytical, Diagnostic and Therapeutic Techniques and Equipment41

Phenomena and Processes83

Disciplines and Occupations2

Information Science12

Health Care4

Acyl7

Recombinant protein7

Tyrosine residues3

Negatively charged2

Peptidyl carrier protein1

Peptides8

Globular1

TMSs3

Saccharomyces1

Topology4

Coli1

Subunit5

Similar proteins1

Molecule2

Mitochondrial3

Intracellular3

Mutation3

Functional1

Sequence1

Positively charged1

Complexes1

Cytoplasmic2

Periplasmic5

Interactions4

Conformational1

Genomes1

Yeast3

Structural8

Extracellular domain2

Helices1

Lipid5

Glycoprotein3

Receptors2

Eukaryotic2

Interact1

Enzyme2

Vertebrate1

Structures4

Substrate1

Crucial2

Kininogen1

Receptor4

Albumin2

Lysine3

Similarity1

Classification1

Binds1

Solute2

Membrane protein1

Chains1

Characterization1

Mechanism2

Affinity1

Pathways2

Polymerase1