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  • Peptide
  • While chemical methylation of an amide bond uses a strong base to generate the imidate, OphA, the precursor protein of the fungal peptide macrocycle omphalotin A, self-hypermethylates amides at pH 7 using S -adenosyl methionine (SAM) as cofactor. (sciencemag.org)
  • A synthetic peptide containing an identified tri-methylated lysine, which corresponds to a previously identified LipL32 epitope, demonstrated significantly reduced immunoreactivity with serum collected from leptospirosis patients compared to the peptide version lacking the tri-methylation. (nih.gov)
  • histone tails
  • Methylation events that weaken chemical attractions between histone tails and DNA increase transcription, because they enable the DNA to uncoil from nucleosomes so that transcription factor proteins and RNA polymerase can access the DNA. (wikipedia.org)
  • patterns
  • Altered DNA methylation patterns in coding strands lead to epigenetic modification. (omicsonline.org)
  • Prior research by the UCLA team found that methylation patterns in antiretroviral-naive adults indicate that their epigenetic age exceeds their chronological age by 14 years . (natap.org)
  • Because antiretroviral-treated people run an increased risk of age-related diseases, these investigators hypothesized that "ART initiation does not restore HIV-perturbed epigenetic patterns to age-appropriate levels, and that DNA methylation ages stay advanced in treated HIV-infected adults compared to chronologically age-matched controls. (natap.org)
  • To test those hypotheses, the UCLA researchers compared methylation patterns in lymphocyte subsets of 15 HIV-positive antiretroviral-treated men (all with an undetectable viral load) and 15 HIV-negative men in the MACS, an ongoing observational study of men who have sex with men (MSM). (natap.org)
  • The UCLA team used Horvath's epigenetic clock to compare DNA methylation patterns in these PBMC sets. (natap.org)
  • They used weighted gene comethylation network analysis (WGCNA) to spot changes in methylation patterns linked to ART initiation. (natap.org)
  • Together with the finding that ART does not restore age-appropriate DNA methylation patterns after 2 years of treatment, these results "suggest that HIV-induced age acceleration, partially mediated through epigenetic changes, may be directly associated with the earlier functional and clinical decline observed in virally suppressed HIV-infected adults on ART. (natap.org)
  • Acceleration of age-associated methylation patterns in HIV-1-infected adults. (natap.org)
  • methyl
  • The resultant genotype and epi-type data is highly comparable in terms of coverage and SNP/methylation site identification to that generated from separate captures for DNA sequencing and methyl-seq. (biomedcentral.com)
  • Methylation of DNA is catalysed by DNA methyl transferase in which methyl donor is Sadenosyl methionine (SAM). (omicsonline.org)
  • methionine
  • This formyl group (and sometimes the methionine residue itself, if followed by Gly or Ser) is removed by the enzyme deformylase. (wikipedia.org)
  • glycosylation
  • recently proposed a novel NMR-based methodology to detect glycosylation of residues in proteins under urea-denaturing conditions. (peerj.com)
  • H3K79
  • In cellular studies, EPZ-5676 inhibited H3K79 methylation and MLL-fusion target gene expression and demonstrated potent cell killing that was selective for acute leukemia lines bearing MLL translocations. (bloodjournal.org)
  • molecule
  • Proline hydroxylation requires ascorbic acid (vitamin C). The most obvious, first effects (gingival and hair problems) of absence of ascorbic acid in humans come from the resulting defect in hydroxylation of proline residues of collagen, with reduced stability of the collagen molecule, causing scurvy. (wikipedia.org)
  • modification
  • The exclusive detection of PTMs on lysine residues within LipL32 from in vivo-isolated L. interrogans implies that infection-generated modification of leptospiral proteins may have a biologically relevant function during the course of infection. (nih.gov)
  • DNA methylation of non-coding region of DNA helps to maintain transcriptional silencing whereas in coding region of DNA it leads to an epigenetic modification. (omicsonline.org)
  • Each one of these proteins has a tail extension, and these tails are the targets of nucleosome modification by methylation. (wikipedia.org)
  • This modification of the proline residue increases the stability of the collagen triple helix. (wikipedia.org)
  • They can have positive and negative effects on transcription and are important in recruiting other transcription factors and histone modification enzymes as well as controlling DNA methylation. (wikipedia.org)
  • Chemical
  • Moreover, as a validation test of this approach, we compare our theoretical computations of the 13 C ε chemical-shift values against existing experimental data, obtained from NMR spectroscopy, for methylated and acetylated lysine residues with good agreement within ∼1 ppm. (peerj.com)
  • detect
  • The two methods were optimized to detect methylation peaks in poly(A)+ mRNA, but the protocol could be adapted to profile any type of RNA. (wikipedia.org)
  • effects
  • It was subsequently shown that the increase in stability is primarily through stereoelectronic effects and that hydration of the hydroxyproline residues provides little or no additional stability. (wikipedia.org)
  • specific
  • An important question now is to understand how methylation varies across a globally diverse collection of wheat germplasm adapted to specific local agricultural niches. (biomedcentral.com)
  • further
  • This paper attempts to review genetic changes attributed to abnormalities in DNA methylation which in turn activate tumoriogenic potential of cells further leading to cancer. (omicsonline.org)
  • nuclear
  • Residues 173-176 are predicted to be a nuclear localization signal KKLK (K-[K/R]-X-[K/R]). FAM98A has two paralogs: FAM98B and FAM98C. (wikipedia.org)