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  • AMPA
  • Molecular and atomic level structural analyses have identified specific S1 and S2 residues that interact directly with ligands, interact with one another in a dimeric configuration, and influence channel gating and desensitization properties of AMPA receptors. (jneurosci.org)
  • We generated a series of GluR6 mutants that converted individual S2 domain residues to their AMPA receptor equivalents. (jneurosci.org)
  • We also examined glutamate (Glu)-, KA-, and AMPA-evoked currents at S2 mutant receptors, the kinetics of recovery from desensitization, and the involvement of putative dimerization residues in GluR6 channel gating and desensitization. (jneurosci.org)
  • Mutation
  • Mutation of the glutamate residue in Walker B motifs (or DExx-boxes) in AAA+ proteins typically reduces ATP hydrolysis by as much as a couple orders of magnitude, but has no effect on ATP binding. (mdpi.com)
  • Presumably, an asymmetry in the reaction profile is induced by the mutation making the deprotonation step involving the serine residue more cleanly rate determining. (ubc.ca)
  • enzymes
  • Further experiments explore the importance of the four strictly conserved residues, AsplO, Asp36, Glul52 and Hisl86, by preparing appropriate mutant enzymes. (ubc.ca)
  • mutants
  • The k[sub cat] values for the D10N and H186N mutants, however, are decreased by three orders of magnitude relative to wild type enzyme implying an important catalytic role for these residues. (ubc.ca)
  • form
  • Methylation of the membrane-bound methyl-accepting chemotaxis proteins (MCP) to form gamma-glutamyl methyl ester residues in MCP. (uniprot.org)
  • human
  • To facilitate the development of antagonists with greater subtype selectivity, we generated molecular models of the glutamate-binding site on rat and human NR2 subunits. (aspetjournals.org)
  • function
  • Chiraniya A, Finkelstein J, O'Donnell M, Bloom LB. A Novel Function for the Conserved Glutamate Residue in the Walker B Motif of Replication Factor C. Genes . (mdpi.com)
  • general
  • Work to understand the reaction mechanism and validate a computational model of asparagine synthetase focused on a conserved glutamate residue which was hypothesized to act as the general base for the deprotonation of ammonia in the transition state. (ufl.edu)