Researchers clone donor egg cell. Medical search. Frequent questions

A year ago, we showed that you could do it with cells from embryos," says Wilmut. (newscientist.com)
Particularly valuable animals could be cloned from adult cells without the uncertainties of crossing them with other animals or tinkering with embryos. (newscientist.com)
More importantly, biotechnologists will for the first time be able to manipulate the genes of cells from farm animals directly before growing them into embryos. (newscientist.com)
But scientists have not managed to isolate such cells from farm animals, and must rely instead on injecting genes randomly into early embryos. (newscientist.com)
A linkurl:report;http://stemcells.alphamedpress.org/cgi/reprint/2007-0252v1.pdf published online today that researchers have cloned human embryos is not that much of an advance, according to one stem cell expert, Douglas Melton, at Harvard University. (the-scientist.com)
Several of the reconstructed oocytes developed as normal embryos, although only one of the blastocysts contained donor DNA or mitochondrial DNA. (the-scientist.com)
In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
They derived several human embryonic stem cell lines from these cloned embryos whose DNA was an exact match to the adult cell that donated the DNA. (news-medical.net)
It may occur accidentally in the case of identical twins, which are formed when a fertilized egg splits, creating two or more embryos that carry almost identical DNA. (wikipedia.org)
The researcher claimed he had created stem cell lines from cloned human embryos. (asianews.it)
Then, in February 2004 he dropped a bombshell, claiming that his SNU research team had cloned the first human embryos and extracted stem cells from them. (koreatimes.co.kr)
Researchers reported in Nature on November 22, 2007, that they successfully isolated 2 embryonic stem cell lines from cloned embryos made using cells from the skin of an adult rhesus macaque. (nih.gov)
Moreover, most early-stage embryos that are produced naturally (that is, through the union of egg and sperm resulting from sexual intercourse) fail to implant and are therefore wasted or destroyed. (wikiquote.org)
The eggs will then be used by scientists from Newcastle and Durham Universities to create embryos from which they will attempt to derive stem cells . (progress.org.uk)
These scientists destroyed the embryos and derived stem cell lines. (nationalrighttolifenews.org)
Most embryos…formed one or two pronuclei at the time of removal from TSA, whereas a slightly higher portion of embryos cleaved…suggesting that some SCNT embryos did not exhibit visible pronuclei at the time of examination… Most cleaved embryos developed to the eight-cell stage…but few progressed to compact morula…and blastocyst. (nationalrighttolifenews.org)
Activation of embryonic genes and transcription from the transplanted somatic cell nucleus are required for development of SCNT embryos beyond the eight-cell stage…Therefore, these results are consistent with the premise that our modified SCNT protocol supports reprogramming of human somatic cells to the embryonic state. (nationalrighttolifenews.org)
A story in News.Com.Au-which runs stories from several Australian newspapers celebrates the cloning breakthrough because it means no embryos are used in the process! (nationalrighttolifenews.org)
But it is an important step in research because it doesn't require the use of embryos in creating the type of stem cell capable of transforming into any other type of cell in the body. (nationalrighttolifenews.org)
The Los Angeles Times has waded in to the junk biology game, assuring us that no embryos are threatened in human cloning-WHEN THE WHOLE POINT OF HUMAN CLONING IS TO CREATE AN EMBRYO! (nationalrighttolifenews.org)
Embryonic stem cells come from human embryos that are three to five days old. (healthline.com)
Interest in using stem cells from cloned human embryos has revived after success by scientists in the United States and Korea. (bioedge.org)
Nature adds that rogue scientists might implant cloned embryos into wombs to create cloned children, a possibility which is widely condemned. (bioedge.org)
Though the jury is out on whether we should try to modify the genes of human embryos, that hasn't stopped researchers from finessing the widely lauded CRISPR gene-editing technique. (cosmosmagazine.com)
It also produces mosaic embryos where some cells get fixed, others don't. (cosmosmagazine.com)
Mitalipov also carries the distinction of being the first to crack the long-standing problem of cloning human embryos and deriving embryonic stem cells. (cosmosmagazine.com)
Out of 58 embryos, 42 showed the normal gene in every cell. (cosmosmagazine.com)
Researchers at collaborating labs in South Korea and China also carried out thorough checks of the embryos' DNA to see if there had been mistakes elsewhere. (cosmosmagazine.com)
Researchers there are working on technology that induces human skin cells to change into the kind of stem cells that have been created by embryos. (cbc.ca)
Some argue that the possibility of mimicking stem cells without acquiring them from embryos, side-steps that moral dilemma. (cbc.ca)
Embryonic stem cells come from embryos, embryonic germ cells from testes, and adult stem cells can come from bone marrow. (cbc.ca)
This new method of generating stem cells does not require embryos as starting points and could be used to generate cells from many adult tissues, such as a patient's own skin cells,' said principal author Andras Nagy, senior investigator at Mount Sinai's Samuel Lunenfeld Research Institute. (cbc.ca)
Today, this technique continues to form the foundation for research on mammalian embryos, including technologies such as transgenic engineering, embryonic stem cell therapy, human in vitro fertilization, mammalian cloning, and knockout engineering. (avma.org)
Using this method of embryo manipulation, he next worked out many aspects of the metabolism and development of eggs and early embryos. (avma.org)
From there, Dr. Brinster became interested in modifying the development of animals and their germ lines, and he went on to become the first person to show that it was possible to colonize a mouse blastocyst with stem cells from older embryos. (avma.org)
Some prohibit only cloning for reproductive purposes and allow the creation of cloned human embryos for research, whereas others prohibit the creation of cloned embryos for any purpose. (who.int)
Citizens disagree about whether we should destroy human embryos for their stem cells-and if so, which embryos, with whose money, under what regulatory guidelines. (eppc.org)
The holy grail of regenerative medicine-whatever one's ethical beliefs about destroying embryos-is to "reprogram" regular cells from one's own body so that individuals can be the source of their own rejection-proof therapies. (eppc.org)
That is to say, we risk turning developed cells into developing embryos, and thus risk engaging in the very activities of embryo destruction and human cloning that we seek to avoid. (eppc.org)
Far more controversial-and for good reason-are stem cells derived from destroyed human embryos. (eppc.org)
There are enough for IVF, but way too few for the hundreds of thousands needed for the extensive trial and error process that will be required to prefect human SCNT, at least to develop the cloned embryo to the point where stem cells can be harvested and eventually, cloned embryos eventually gestated to birth. (cbc-network.org)
Thus, while we hear much about helping the infertile and trying to obtain stem cells from cloned embryos for use in medical treatments, I am convinced if we found non-cloning sources for both objectives, many biotechnologists would just shrug and keep on cloning. (cbc-network.org)
Using a cell-sorting machine that can separate out the marked cells, the team obtained reproductive cells from mouse ovaries and showed that the cells would generate viable egg cells that could be fertilized and produce embryos. (cbc-network.org)
Eligible Australian women with a diagnosis of mitochondrial disease may have access to mitochondrial donation under amendments to the Research Involving Human Embryos Act 2002 and the Prohibition of Human Cloning for Reproduction Act 2002 which took effect in October 2022. (nhmrc.gov.au)
This technique was developed by modifying the conventional cloning technology*2 and is the world's first successful induction of gene activation*3, which is specifically expressed in early embryos from genomes of Oryx dammah, now extinct in the wild. (mynewsdesk.com)
The research team succeeded in inducing the activation of embryonic genes*4, which is an essential reprogramming phenomenon, with various animal somatic nuclei in mouse embryos arrested at the early stage, which has not been used in conventional cloning techniques. (mynewsdesk.com)
2 Cloning technology: Development of reconstructed embryos produced by transplanting cell nuclei into pre-fertilized eggs is induced by activation stimulation, and then the reconstructed embryos are to be implanted in oocytes to generate cloned animals that share identical genomes as the donor cells. (mynewsdesk.com)
The main issue as to whether or not human cloning is possible through the splitting of embryos began in 1993 when experimentation was done at George Washington University Medical Center in Washington D. C. There Dr. Jerry Hall experimented with the possibility of human cloning and began this moral and ethical debate. (benjaminbarber.org)
They attempted to create seventeen human embryos in a laboratory dish and when it had grown enough, separated them into forty-eight individual cells. (benjaminbarber.org)
Two of the separated cells survived for a few days in the lab developed into new human embryos smaller than the head of a pin and consisting of thirty-two cells each (Brownlee 24). (benjaminbarber.org)
Cloning embryos is different from the genetic process of in vitro fertilization, but still holds many similarities with it. (benjaminbarber.org)
When a fertilised egg separates into two or more embryos with almost identical DNA, these twins are created. (geminigenetics.com)
Stem cells may be derived from adult tissues but the most potent are extracted from developing human embryos. (edu.au)
The environmental group argued that Brüstle's work was "contrary to public order" because embryos were destroyed to gather the stem cells used. (ox.ac.uk)
The judgment effectively supports the Greenpeace view and imposes a ban on patenting work that uses embryonic stem cells on the grounds that it represents an immoral "industrial" use of human embryos. (ox.ac.uk)
Stem cells from embryos may provide the holy grail of medicine. (ox.ac.uk)
Stem cells from embryos are very special building blocks. (ox.ac.uk)
An excess of embryos is produced during in vitro fertilisation to prevent women having extra cycles of egg harvesting that pose risks to their health. (ox.ac.uk)
Instead of becoming "biowaste" these embryos could be used to produce embryonic stem cells. (ox.ac.uk)

After growing and dividing for a week or so in a laboratory culture dish, the fused cell forms an early embryo called a blastocyst, which Wilmut's team implants into a surrogate mother. (newscientist.com)
A blastocyst (cloned or not), because it lacks any trace of a nervous system, has no capacity for suffering or conscious experience in any form - the special properties that, in our view, spell the difference between biological tissue and a human life worthy of respect and rights. (wikiquote.org)
After many divisions in culture, this single cell forms a blastocyst (an early stage embryo with about 100 cells) with almost identical DNA to the original donor who provided the adult cell - a genetic clone. (eurostemcell.org)
To produce Dolly, the cloned blastocyst was transferred into the womb of a recipient ewe, where it developed and when born quickly became the world's most famous lamb. (eurostemcell.org)
In therapeutic cloning, the blastocyst is not transferred to a womb. (eurostemcell.org)
Instead, embryonic stem cells are isolated from the cloned blastocyst. (eurostemcell.org)
Stem cells were then derived from the inner cell mass of the blastocyst. (scienceblog.com)
The Korean researchers who performed this stem cell research improved upon their protocols that yielded the first embryonic stem cell line from a cloned human blastocyst. (scienceblog.com)
Nor do only the cells of the inner cell mass of the blastocyst become the later adult and none of the cells from the inner cell mass become part of the placenta, umbilical cord, etc. (lifeissues.net)
Moreover, Dr. Brinster first demonstrated that teratocarcinoma cells could combine with blastocyst cells to form adult chimeric mice, establishing the feasibility of this approach to change the genetic character of mice. (avma.org)
Most researchers obtain embryonic stem cells from the inner mass of a blastocyst, an embryonic stage when a fertilized egg has divided into 128 cells. (jcpa.org)
The stem cells derived from the inner mass of a blastocyst lack the ability to form a fetus when implanted into a woman, but are self-renewing and can be maintained for long periods of time in the laboratory as undifferentiated stem cells. (jcpa.org)
Therapeutic cloning involves the creation of an early-stage embryo (blastocyst) and the removal of stem cells from the developing embryo. (geminigenetics.com)

Using eggs from adult women who had previously donated for successful fertility treatments, the researchers used SCNT to transfer DNA into the egg cells. (the-scientist.com)
One cloning technology that has been developed for mammalian and human cells is somatic cell nuclear transfer (SCNT). (news-medical.net)
SCNT is a method of cloning mammalian cells that can be used to create personalized embryonic stem cells from an adult animal or human. (news-medical.net)
This has led to a lot of interest in SCNT, which is best known as the method used to pioneer whole animal cloning technology, such as Dolly the sheep. (news-medical.net)
But SCNT can also be used to clone human cells for transplant or other therapies. (news-medical.net)
This was the first successful reprogramming of human somatic cells into embryonic stem cells using a cloning technique, SCNT. (news-medical.net)
Another successful attempt at human SCNT was made using cells from two adult males. (news-medical.net)
The artificial cloning of organisms, sometimes known as reproductive cloning, is often accomplished via somatic-cell nuclear transfer (SCNT), a cloning method in which a viable embryo is created from a somatic cell and an egg cell. (wikipedia.org)
The use of embryonic stem cells, which can be produced through SCNT, in some stem cell research has attracted controversy. (wikipedia.org)
Once the SCNT is done, the cloning is over. (nationalrighttolifenews.org)
The cloning is completed when the SCNT is accomplished. (nationalrighttolifenews.org)
Cloning, or somatic cell nuclear transfer (SCNT), is the technique used to produce Dolly the sheep, the first animal to be produced as a genetic copy of another adult. (eurostemcell.org)
This method is called "somatic cell nuclear transfer" or SCNT. (scienceblog.com)
Indeed, if passed, Hatch/Feinstein/Kerry would explicitly legalize doing in humans the very cloning procedure -- somatic cell nuclear transfer (SCNT) -- that was used to make Dolly the sheep . (lifeissues.net)
Comment: Indeed, if passed, "total cloning bans" H.R. 534, H.R. 234, H.R. 916, and S. 245 would not ban anything either - not even the SCNT cloning technique that was used to make Dolly the sheep. (lifeissues.net)
Dolly was the first mammal cloned from specialized adult (somatic) cells with the technique of somatic cell nuclear transfer (SCNT). (asu.edu)
Here's what I mean: Each try at somatic cell nuclear transfer (SCNT) cloning to manufacture a human being (or, member of the species Homo sapiens , if you prefer) requires a human egg. (cbc-network.org)
The promise of the SCNT method is that the nucleus of a patient's skin cell, for example, could be used to create pluripotent cells that might be able to repair a part of that patient's body. (pharmaceuticalintelligence.com)
One attraction of SCNT has always been that the genetic identity of the new pluripotent cell would be the same as the patient's, since the transplanted nucleus carries the patient's DNA. (pharmaceuticalintelligence.com)
Stanford University have raised the possibility in the past that the immune system of a patient who received SCNT-derived cells might still react against the cells' mitochondria, which act as the energy factories for the cell and have their own DNA. (pharmaceuticalintelligence.com)
This reaction could occur because cells created through SCNT contain mitochondria from the egg donor and not from the patient, and therefore could still look like foreign tissue to the recipient's immune system. (pharmaceuticalintelligence.com)
When transplanted back into the nucleus donor strain, the cells were rejected although there were only two single nucleotide substitutions in the mitochondrial DNA of these SCNT-derived cells compared to that of the nucleus donor. (pharmaceuticalintelligence.com)
Until recently, researchers were able to perform SCNT in many species, but not in humans. (pharmaceuticalintelligence.com)
When scientists at the Oregon Health and Science University announced success in performing SCNT with human cells last year, it reignited interest in eventually using the technique for human therapies. (pharmaceuticalintelligence.com)
Although many stem cell researchers are focused on a different method of creating pluripotent stem cells, called induced pluripotent stem cells, there may be some applications for which SCNT-derived pluripotent cells are better suited. (pharmaceuticalintelligence.com)
The immunological reactions reported in the new paper will be a consideration if clinicians ever use SCNT-derived stem cells in human therapy, but such reactions should not prevent their use. (pharmaceuticalintelligence.com)
This research informs the medical community of the margin of safety that would be required if, in the distant future, researchers need to use SCNT to create pluripotent cells to treat someone. (pharmaceuticalintelligence.com)
The technique used by Drs. Mitalipov, Paula Amato, M.D. , and their colleagues in OHSU's Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynecology, is a variation of a commonly used method called somatic cell nuclear transfer, or SCNT. (ohsu.edu)

If artificial cloning and natural cloning both lead to the same result, which is the formation of a clone, that is, an organism with identical or nearly identical genes to another organism, then the plight of This creation is very different between the two creatures. (wikipedia.org)
A cloned embryo-like a natural embryo-is an individual organism, a member of its (in this case, human) species. (nationalrighttolifenews.org)
These stem cells are genetically matched to the donor organism, holding promise for studying genetic disease. (eurostemcell.org)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
A clone is an organism that is a genetic copy of an existing one. (who.int)
Stem cell research is, in part, a quest to understand cellular differentiation, the process by which a human being develops from one fertilized cell into a multicellular organism composed of over 200 different cell types - for example muscle, nerve, blood cell, or kidney. (jcpa.org)
At the top of the list comes the zygote-a fertilized egg, which of course has the ability to divide and differentiate into all cell types in the body and create a new organism. (thefutureofthings.com)
The first three divisions of the zygote give birth to eight totipotent cells, each of which also has the ability to become an entire organism. (thefutureofthings.com)
1 Reprogramming: Reprogramming is the process that adult somatic cells acquire the ability to differentiate into all cell types of an organism. (mynewsdesk.com)
A new organism is created by asexual reproduction using a duplicate of a single cell from the parent organism. (geminigenetics.com)
This is the most known form of cloning and involves creating a genetically identical replica of a whole organism. (geminigenetics.com)
The process of reproductive cloning involves the nucleus of a somatic (body) cell from a donor organism to be cloned being transferred into an egg cell whose nucleus (genetic material) has been removed. (geminigenetics.com)
XI - embryonic stem cells: embryonic cells that are capable of modifying the cells of any organism tissue. (hinxtongroup.org)
… "embryo" means a human organism during the first 56 days of its development following fertilization or creation, excluding any time during which its development has been suspended, and includes any cell derived from such an organism that is used for the purpose of creating a human being. (hinxtongroup.org)

The difference with Dolly is that all her DNA originated in a cell from the udder of an adult sheep. (newscientist.com)
Dolly was the only lamb born from 277 fusions of oocytes with udder cells. (newscientist.com)
In 1996, Dolly the sheep achieved notoriety for being the first mammal cloned from a somatic cell. (wikipedia.org)
Researchers have been hoping to harness the therapeutic potential of cloning ever since the cloning of Dolly the sheep in 1997. (nih.gov)
When an embryo like this is implanted into a uterus, as with Dolly, the process is called reproductive cloning. (nih.gov)
After the birth of Dolly in 1996, the first successfully cloned mammal, excitement filled the scientific community and led to further investigation and development in the field of genetic engineering (Kolata, 1997). (umass.edu)
The resulting egg was implanted in the womb of a third sheep, and the result was Dolly, the first clone of a mammal. (usf.edu)
As with Dolly, the women's nuclear DNA had been removed from these eggs before the man's DNA was injected. (usf.edu)
Dolly, the first mammal to be genetically cloned from adult cells, poses for the camera in 1997 at the Roslin Institute in Edinburgh, Scotland. (usf.edu)
Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)
Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
Campbell and his team also cloned a sheep from adult cells in 1996, which they named Dolly. (asu.edu)
The cloning of 'Dolly The Sheep' in 1996 by the Rosalind Institute in Scotland, UK, is the most recognised example of reproductive cloning. (geminigenetics.com)

General Assembly the following year,3 and the World Medical Association's Resolution on Cloning, endorsed in 1997, have confronted the issue but lack binding legal force. (who.int)
WHA50.37 of 1997 argues that human cloning is ethically unacceptable and contrary to human integrity and morality. (who.int)

By transferring adult cell DNA into an embryonic stem cell, it is possible to create a line of immortal embryonic cells that are able to develop into any type of adult cell, genetically identical to the donor. (news-medical.net)
Cloning is the process of producing individual organisms with identical genomes, either by natural or artificial means. (wikipedia.org)
Another example of artificial cloning is molecular cloning, a technique in molecular biology in which a single living cell is used to clone a large population of cells that contain identical DNA molecules. (wikipedia.org)
In another strategy, called therapeutic cloning, the embryo can instead be used to create stem cells that are genetically identical to a patient. (nih.gov)
Before this new study was published, Nature asked another group of researchers to confirm that the stem cells were genetically identical to the donor skin cells. (nih.gov)
In biology , cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria , insects or plants reproduce asexually . (wikiquote.org)
But they showed, for the first time, that it is possible to create cloned embryonic stem cells that are genetically identical to the person from whom they are derived. (nationalrighttolifenews.org)
Cloning, the process of producing a genetically identical individual using the DNA of another individual, has been used over the past decade to revive extinct species. (umass.edu)
The experiment resulted in the birth of a Bucardo that was genetically identical to the donor cell. (umass.edu)
Another long-term hope for therapeutic cloning is that it could be used to generate cells that are genetically identical to a patient. (eurostemcell.org)
The term "clone", from the Greek word for twig, denotes a group of identical entities. (who.int)
In sexual reproduction, clones are created when a fertilized egg splits to produce identical (monozygous) twins with identical genomes. (who.int)
Thus, the clone would be genetically identical to the nucleus donor only if the egg came from the same donor or from her maternal line. (who.int)
Beyond this scientific interest, the commercial concern in animal cloning focuses on replicating large numbers of genetically identical animals, especially those derived from a progenitor that has been modified genetically. (who.int)
As the fertilized egg divides from one cell into two, physicians can separate these two cells and implant each one of them into a woman's uterus to generate two genetically identical children. (jcpa.org)
It is a method that involves the production of a group of identical cells or organisms that all derive from a single individual (Grolier 220). (benjaminbarber.org)
The second method of cloning a human involves taking cells from an already existing human being and cloning them, in turn creating other individuals that are identical to that particular person. (benjaminbarber.org)
The word "cloning" refers to a variety of procedures that may be used to create biological copies that are genetically identical to the original. (geminigenetics.com)
Asexual reproduction is a natural method used by certain plants, bacteria, and single-celled creatures to create genetically identical offspring, i.e. clones. (geminigenetics.com)
Humans and other mammals may produce natural clones, commonly referred to as identical twins. (geminigenetics.com)
The resulting embryo is then implanted into a surrogate mother, resulting in the birth of an animal genetically identical to the body cell donor. (geminigenetics.com)
Pet cloning is the process where a genetically identical twin is created of your original animal companion. (geminigenetics.com)
The surrogate mum carries the cloned pet for the gestation period and once ready, gives birth to the clone who will be an identical genetic twin to the original pet whose skin sample was used to make the nucleus of the donor egg cell. (geminigenetics.com)

Religious groups believe that the raw material from which stem cells are sourced are themselves forms of human life, and by creating little chunks of humans in Petri dishes, scientists are, critics believe, playing God. (koreatimes.co.kr)
Treatment at the Newcastle NHS Fertility Centre, which will offer the scheme, usually costs £3,500, if a woman agrees to surrender half of her derived eggs to scientists the fee will be halved. (progress.org.uk)
Until now scientists have been restricted to using 'left-over' eggs from IVF treatment, these are usually poor quality and are already older than those that will be donated through the new scheme. (progress.org.uk)
Discussing the planned public consultation Angela McNab, chief executive of the HFEA, said, 'We know there are a wide variety of views on the subject of donating eggs for research and we anticipate a strong response to the consultation from professional groups, scientists, clinicians and patients as well as the public. (progress.org.uk)
Scientists have used cloning technology to transform human skin cells into embryonic stem cells, an experiment that may revive the controversy over human cloning. (nationalrighttolifenews.org)
The method described on Wednesday by Oregon State University scientists in the journal Cell, would not likely be able to create human clones, said Shoukhrat Mitalipov, senior scientist at the Oregon National Primate Research Center. (nationalrighttolifenews.org)
Since stem cells have the ability to turn into various other types of cells, scientists believe that they can be useful for treating and understanding diseases. (healthline.com)
Scientists have recently discovered how to turn adult stem cells into pluripotent stem cells. (healthline.com)
To create iPSCs, scientists genetically reprogram the adult stem cells so they behave like embryonic stem cells. (healthline.com)
Scientists are hoping that the cells can be made from someone's own skin to treat a disease. (healthline.com)
From these fibroblasts, the scientists extracted the DNA of the Bucardo and injected it into unfertilized eggs of a domestic goat. (umass.edu)
Another similar attempt was performed in 2011 when scientists from the Royan Institute for Reproductive Biomedicine cloned the near-extinct Esfahan Mouflon. (umass.edu)
Scientists are unlikely to rush into creating human embryonic stem cell lines. (bioedge.org)
Scientists have isolated the first human embryonic stem cell lines specifically tailored to match the nuclear DNA of patients, both males and females of various ages, suffering from disease or spinal cord injury. (scienceblog.com)
The work also moves scientists one step closer to the goal of transplanting healthy cells into humans to replace cells damaged by diseases such as Parkinson's and diabetes. (scienceblog.com)
From the 31 nuclear-transfer blastocysts, the scientists derived 11 stem cell lines. (scienceblog.com)
Scientists have been all abuzz in the last few years over stem cells - cellular magicians that promise to dazzle and amaze. (cbc.ca)
Scientists say embryonic stem cells are the most useful type because they have the potential to become any type of cell within the body. (cbc.ca)
Scientists are fascinated by the ability of stem cells to become any type of cell. (cbc.ca)
They then used a Scientists gene-edit piglets, bringing transplants to humans closer standard cloning technique to insert the edited DNA into egg cells that were placed into a surrogate mother. (dailymaverick.co.za)
Having just tested the children born as a result of this procedure, the scientists have confirmed that the children's cells contain mitochondria, and hence genes, from two women as well as their fathers. (bbc.co.uk)
Eighteen years ago, scientists in Scotland took the nuclear DNA from the cell of an adult sheep and put it into another sheep's egg cell that had been emptied of its own nucleus. (usf.edu)
So ideally scientists would like to be able to extract DNA from the cells of older people - not just cells from infants - to create therapies for adult diseases. (usf.edu)
In the 18 years since researchers cloned a sheep, scientists have found another way to produce cloned human cell lines. (usf.edu)
In principle, scientists could produce a series of cell lines that would allow a close match for the majority of would-be cell recipients - just as transplant surgeons currently seek a close match for organ donors. (usf.edu)
1. Cloning is an umbrella term traditionally used by scientists to describe different processes for duplicating biological material. (who.int)
This policy is similar to that of other countries, including Israel, where scientists are funded by Government to study embryonic stem cells despite the aforementioned bioethical issue. (jcpa.org)
Gurdon's research built on the work of Thomas King and Robert Briggs in the United States, who in 1952 published findings that indicated that scientists could take a nucleus from an early embryonic cell and successfully transfer it into an unfertilized and enucleated egg cell. (asu.edu)
While regarded by many top scientists as the Holy Grail of medicine, others consider embryonic stem-cell research sacrilegious. (thefutureofthings.com)
A number of large biotech companies and scientists are looking toward stem cells as the basis for a therapeutic solution to cure such illnesses as blindness, diabetes and spinal cord injuries. (thefutureofthings.com)
Long before the controversy emerged over human embryonic stem cells, scientists and doctors began using first-generation stem cells from adult bone marrow. (eppc.org)
But they are also less equipped to produce every cell type of the body and less able to reproduce themselves indefinitely, which makes them less appealing to scientists interested in basic research. (eppc.org)
In July 2005, for example, scientists announced that they had engineered adult mouse stem cells into usable mouse eggs, a technique that might one day allow for the creation of human eggs from ordinary human cells. (eppc.org)
Umbilical Cord Blood Storage Company Sees Growth Umbilical Cord Stem Cell Storage Resources Arkansas House Votes to Create Umbilical Cord Stem Cell Bank Scientists Claim They Discovered Molecule That Might Help Embryonic Stem Cells Grow US Senate Vote for. (physiciansforlife.org)
The advance, if confirmed, might provide a new source of eggs for treating infertility, though scientists say it is far too early to tell if the work holds such promise…The new research, by a team led by the biologist Jonathan L. Tilly, depends on a special protein found to mark the surface of reproductive cells like eggs and sperm. (cbc-network.org)
These scientists experimented eagerly in aims of learning how to clone human. (benjaminbarber.org)
Common answers to the puzzling questions about humans and cloning are still trying to be answered today, and scientists and the public are eager to learn all they can about cloning. (benjaminbarber.org)
In the future, scientists might also lessen the immune reaction by using eggs from someone who is genetically similar to the recipient, such as a mother or sister. (pharmaceuticalintelligence.com)
Scientists at Oregon Health & Science University and the Oregon National Primate Research Center (ONPRC) have successfully reprogrammed human skin cells to become embryonic stem cells capable of transforming into any other cell type in the body. (ohsu.edu)

It is also our view that there are no sound reasons for treating the early-stage human embryo or cloned human embryo as anything special, or as having moral status greater than human somatic cells in tissue culture. (wikiquote.org)
Various cloned animals have been created with the reprogramming technology that restores cells to the undifferentiated state by transplanting differentiated adult somatic cells into oocytes, and it has been also expected to revive endangered or extinct species. (mynewsdesk.com)
In particular, genomic reprogramming resets the epigenetic information accumulated in the adult somatic cells, and the cells revert back to an early embryonic state. (mynewsdesk.com)

The researchers, at Mount Sinai Hospital in Toronto, say the development could bring personalized regenerative medicine a step closer to reality. (cbc.ca)
The Korea Centers for Disease Control and Prevention has registered "Sooam-hES-1" human stem cell line to the National Stem Cell Bank of National Centre for Stem Cell and Regenerative Medicine. (sooam.com)
Therapeutic cloning refers to the production of embryonic stem cells for medicinal reasons, for example regenerative medicine and tissue replacement. (geminigenetics.com)
While there is much work to be done in developing safe and effective stem cell treatments, we believe this is a significant step forward in developing the cells that could be used in regenerative medicine. (ohsu.edu)

If the same could be achieved in humans, it would mean that each of us could have clones of ourselves made from our own tissue. (newscientist.com)
All humans start out as only one cell. (healthline.com)
In most countries, it is illegal to attempt reproductive cloning in humans. (eurostemcell.org)
It is not clear whether PERVs would infect humans who receive pig organs, but lab studies have shown human cells can be infected by the viruses in a dish. (dailymaverick.co.za)
It also means that finally getting the sheep technology to work with cells from adult humans may not turn out to be a turning point for this technology, after all. (usf.edu)
In rodents, and even in some preliminary trials in humans, human embryonic stem cells have been shown to bridge gaps in spinal cord injuries , allowing restoration of motor functions. (thefutureofthings.com)
Cloning humans has recently become a possibility that seems much more feasible in today's society than it was twenty years ago. (benjaminbarber.org)
It is not known when or how cloning humans really became a possibility, but it is known that there are two possible ways that we can clone humans. (benjaminbarber.org)
There is no doubt that many problems involving the technological and ethical sides of this issue will arise and will be virtually impossible to avoid, but the overall idea of cloning humans is one that we should accept as a possible reality for the future. (benjaminbarber.org)
Cloning humans is an idea that has always been thought of as something that could be found in science fiction novels, but never as a concept that society could actually experience. (benjaminbarber.org)
Stem cell technology in humans derives from earlier and complementary work in animal studies. (edu.au)
The findings reveal a likely, but surmountable, hurdle if such therapies are ever used in humans, the researchers said. (pharmaceuticalintelligence.com)

First, they extract immature, unfertilised egg cells called oocytes from the ovaries of ewes. (newscientist.com)
The cloning method is based on the fact that cytoplasmic factors in mature, metaphase II oocytes are able to reset the identity of a transplanted adult cell nucleus to an embryonic state. (news-medical.net)
The adult cell nuclei were transferred into metaphase-II stage human oocytes, producing a karyotypically normal diploid embryonic stem cell line from each of the adult male donor cells. (news-medical.net)
The therapeutic potential of cloned human cells has been demonstrated by another study using human oocytes to reprogram adult cells of a type 1 diabetic. (news-medical.net)
In their new paper, Science author Woo Suk Hwang from Seoul National University in Seoul, Korea and colleagues replaced the nuclei from donated oocytes with nuclei from skin cells from male and female patients, ranging in age from 2 to 56, who had spinal cord injuries, juvenile diabetes and the genetic disease "congenital hypogamma-globulinemia. (scienceblog.com)
From the 185 donated oocytes, endowed with the genetic material from a different person (or in one case, the same person), the researchers report development of 31 hollow balls of cells called "human nuclear-transfer blastocysts. (scienceblog.com)
The ten additional new lines resulted from nuclear transfer with skin cells of males or females and oocytes from biologically-unrelated females. (scienceblog.com)
The extracted samples from Pahu have been sent to a laboratory at the Bogor Institute of Agriculture (IPB) on the island of Java, where the egg cells, or oocytes, will go through a maturing process before any attempt at fertilization. (mongabay.com)

The mitochondrial DNA of the stem cells, however, matched the donor egg's mitochondrial DNA. (news-medical.net)
mitochondria in an egg donated by another woman (the mitochondrial donor). (nhmrc.gov.au)
As egg cells contribute mitochondria to the next generation, this approach minimises the risk of a prospective mother transmitting mitochondrial disease to her child. (nhmrc.gov.au)

Elaboration of an international convention against reproductive cloning of human beings has been under consideration in the United Nations since December 2001 when the subject was included in the agenda of the fifty- sixth session as a supplementary agenda item at the request of France and Germany. (who.int)
2. Over the years, the international community has tried without success to build a consensus on an international convention against the reproductive cloning of human beings. (who.int)
3. Creating awareness among ministries of health in the African Region will provide them with critical and relevant information on the reproductive cloning of human beings and its implications to the health status of the general population. (who.int)
7. The WHO Regional Committee for Africa is invited to review this document for information and guidance concerning reproductive cloning of human beings. (who.int)
3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)

The fall from grace of stem cell scientist Hwang Woo-suk would be as spectacular as his meteoric ascent. (koreatimes.co.kr)
A Korean television station whose investigative report was the nail in the coffin that prompted human cloning scientist Hwang Woo-suk to admit he lied about egg donations his researchers made says he may have lied about the results of his research as well. (blogspot.com)

Retrieved on December 04, 2023 from https://www.news-medical.net/life-sciences/Cloning-Human-Cells.aspx. (news-medical.net)

The egg is artificially stimulated to divide and behave in a similar way to an embryo fertilised by sperm. (eurostemcell.org)
Should there be a 'dead donor rule' for sperm donation? (bioedge.org)
in the current report, CRISPR was added to eggs at an earlier stage, at the same time as the sperm. (cosmosmagazine.com)
The sperm came from a donor with hypertrophic cardiomyopathy. (cosmosmagazine.com)
The researchers co-injected the affected donor's sperm together with the CRISPR editor. (cosmosmagazine.com)
Conservationists say they hope to eventually fertilize Pahu's eggs with sperm from captive Sumatran males, with one of the Sumatran females then serving as a surrogate to hopefully bring a baby to term. (mongabay.com)
We make every effort to keep the survival of the Sumatran rhinos in Kalimantan [Indonesian Borneo], one of which is with assisted reproductive technology such as in-vitro fertilization with sperm from Sumatran rhinos in Way Kambas National Park, stem cells, and cloning," Satyawan Pudyatmoko, the environment ministry's director-general of natural resources and ecosystem conservation, said in the now-deleted statement. (mongabay.com)
The egg cells, when injected into mice, generated follicles, the ovarian structure in which eggs are formed, as well as mature eggs, some of which had a single set of chromosomes, a signature of eggs and sperm. (cbc-network.org)
Another technique, pronuclear transfer (PNT), involves the fertilisation of a mother's and a donor egg with the father's sperm and transfer of the parents' nuclear DNA from a fertilised egg to a fertilised donor egg that has the nucleus removed. (nhmrc.gov.au)
It involves taking an egg from the woman and taking sperm from the man. (benjaminbarber.org)
Xenograft-derived sperm where then injected into donor eggs and implanted into a total of 53 female surrogate monkeys. (asianscientist.com)
In the wild type donor group, we can get mature sperm in two years. (asianscientist.com)

In-vitro maturation of the ovocyte uses the DNA from the female, inseminates the egg, produces a zygote and then editing reagents are introduced into the zygote. (bifconference.com)
Harvesting her eggs for possible use in in-vitro fertilization is thus seen as crucial in the captive-breeding program, which since 2012 has seen just three rhinos born in captivity at Way Kambas. (mongabay.com)
Egg harvesting and Embryonic Stem-cell Research Pose Serious Threat to Women's Health A congressional hearing last Thursday raised awareness on the risks to women's health and fertility by in vitro fertilization (IVF), human cloning, embryonic stem-cell. (physiciansforlife.org)
Many sources state that cloning is just simply an extension of in vitro fertilization, but the root of cloning goes further than that. (benjaminbarber.org)
it helped uncover the secrets of cancer and the effects of the atom bomb, and led to important advances like cloning, in vitro fertilization, and gene mapping. (oprah.com)
… "human clone" means an embryo that, as a result of the manipulation of human reproductive material or an in vitro embryo, contains a diploid set of chromosomes obtained from a single - living or deceased - human being, fetus, or embryo. (hinxtongroup.org)
In vitro, experiments performed on human HEC-1-A and AN3CA cells showed that overexpression of TRIM33 inhibited the proliferation, migration and invasion of EC cells, whereas TRIM33 knockdown resulted in the opposite results. (bvsalud.org)

Cloning of human cells is a technology that holds the potential to cure many diseases and provide a source of exactly matched transplant tissues and organs. (news-medical.net)
This long-sought technique may eventually let doctors create replacement cells for a wide variety of tissues from bits of a patient's own skin. (nationalrighttolifenews.org)
These stem cells come from developed organs and tissues in the body. (healthline.com)
In addition to their ability to supply cells at the turnover rate of their respective tissues, they can be stimulated to repair injured tissue caused by liver damage, skin abrasions and blood loss. (thefutureofthings.com)
The ability of our body to regenerate some of its tissues is largely owed to the reserves of adult stem cells. (thefutureofthings.com)
Today, we can derive stem cells from a range of adult and newborn tissues: liver cells, kidney cells, brain cells, fat cells, and umbilical cord blood. (eppc.org)
By this technique, the team established primary cultured cells using tissues collected from postmortem Oryx dammah, a species extinct in the wild, and succeeded in activating embryonic genes from their cell nuclei. (mynewsdesk.com)
Numerous biological components, including genes, cells, tissues, and even complete creatures like sheep, have been cloned by researchers, and now cat, dog and equine cloning is widely and reliably available via international companies such as our partner, ViaGen Pets & Equine. (geminigenetics.com)
Mouse cells and tissues created through nuclear transfer can be rejected by the body because of a previously unknown immune response to the cell's mitochondria, according to an international study in mice by researchers at the Stanford University, MIT and colleagues in Germany and England. (pharmaceuticalintelligence.com)
The hope has been that this would eliminate the problem of the patient's immune system attacking the pluripotent cells as foreign tissue, which is a problem with most organs and tissues when they are transplanted from one patient to another. (pharmaceuticalintelligence.com)
They can be used to replace dead or damaged cells, tissues or organs. (ox.ac.uk)

These cells have been sought after as potential therapies for diseases ranging from heart disease to Parkinson's to cancer. (news-medical.net)
Moreover, they can be used independently to research how, for example, diseases attack cells. (koreatimes.co.kr)
Since embryonic stem cells have the ability to form virtually any cell type in the body, those taken from a cloned embryo could potentially be used to treat many diseases. (nih.gov)
Claims that you could clone individual treatments of human beings to treat common diseases like diabetes, suggests you need a huge supply of human eggs. (wikiquote.org)
The stem cells could be studied in the laboratory to help researchers understand what goes wrong in diseases like these. (eurostemcell.org)
Since then, there has been a flurry of announcements about developments in stem cell research and hints of promising treatments for diseases such as Alzheimer's, Parkinson's and cancer. (cbc.ca)
In May 2007, Ontario and California announced a $30-million stem cell research deal aimed at finding new therapies for those diseases. (cbc.ca)
Dolly's birth set off a huge outpouring of ethical concern - along with hope that the same techniques, applied to human cells, could be used to treat myriad diseases. (usf.edu)
While there is a great deal published on the potential medical applications of stem cell research to treat or cure diseases such as diabetes, Alzheimer's disease, Parkinson's disease, cancer, and heart disease, much less has been published on the future impact of stem cell research in reproductive medicine. (jcpa.org)
Already, non-embryonic stem cells are being used to treat a variety of diseases-most notably certain cancers of the blood. (eppc.org)
The recovered stem cells can then be used in the treatment of diseases and to aid the recovery of injuries. (geminigenetics.com)
Diseases or conditions that might be treated through stem cell therapy include Parkinson's disease, multiple sclerosis, cardiac disease and spinal cord injuries. (ohsu.edu)
This is a remarkable accomplishment by the Mitalipov lab that will fuel the development of stem cell therapies to combat several diseases and conditions for which there are currently no treatments or cures," said Dr. Dan Dorsa, Ph.D. , OHSU Vice President for Research. (ohsu.edu)
Most importantly, embryonic stem cells may allow transplantation to be used to treat common diseases like heart attack, Alzheimer's Disease, diabetes, Parkinson's Disease and stroke. (ox.ac.uk)

Last year, Hwang's team said it successfully cloned a human embryo from embryonic stem cells. (blogspot.com)

One important distinction is that while the method might be considered a technique for cloning stem cells, commonly called therapeutic cloning, the same method would not likely be successful in producing human clones otherwise known as reproductive cloning. (ohsu.edu)

Wilmut and his colleagues fuse the empty oocyte with the donor cell by bringing them together and subjecting them to an electric current. (newscientist.com)
Each of the 11 new human embryonic stem cell lines was created by transferring the nuclear genetic material from a non-reproductive cell of a patient into a donated egg, or "oocyte," whose nucleus had been removed. (scienceblog.com)
Oocyte donors and patients who donated non-reproductive cells were all unpaid volunteers. (scienceblog.com)
In a Science "Policy Forum" related to the team's latest findings, David Magnus and Mildred Cho from Stanford University in Palo Alto, CA discuss international oversight and ethical issues in oocyte donation, including the need to promote realistic expectations of the outcomes of stem cell research. (scienceblog.com)
The single cell line generated in the 2004 Science paper resulted from nuclear transfer in which the oocyte and non-reproductive ("somatic") cell came from the same healthy female. (scienceblog.com)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
To do this, DNA is first taken from a donor oocyte (egg cell). (bifconference.com)
Then the diploid nucleus from the edited somatic cell is moved into the oocyte and implanted into a surrogate dam, said Van Eenennaam. (bifconference.com)

The team that isolated the embryonic stem cell lines was led by Dr. Shoukhrat Mitalipov at Oregon Health and Science University in Portland. (nih.gov)
The stem cells, the researchers showed, could turn into heart or nerve cells in the laboratory, and had other characteristics of established embryonic stem cell lines. (nih.gov)
To date, no human embryonic stem cell lines have been derived using therapeutic cloning, so both these possibilities remain very much in the future. (eurostemcell.org)
One group from CHA University in Seoul reported in Cell Stem Cell in April that it had managed to use cell nuclei from two men, aged 35 and 75, to create embryonic stem cell lines. (bioedge.org)
There have been many false dawns in the field of embryonic stem cell research, but these results seem to confirm that it is possible to use adult cells to create genetically matched stem cell lines. (bioedge.org)
The stem cell lines produced from patients with disease will likely display characteristics of the disease, so they will probably not be appropriate for direct use in treating patients. (scienceblog.com)
The researchers generated these stem cell lines ten times more efficiently than in their 2004 Science study, using improved laboratory methods. (scienceblog.com)
One of the next preclinical steps, according to the authors, is to evaluate, in the lab, differentiated patient-specific human embryonic stem cell lines for immune-system tolerance, therapeutic efficacy and safety. (scienceblog.com)

Cloning has been proposed as a means of reviving extinct species. (wikipedia.org)
depictions commonly involve themes related to identity, the recreation of historical figures or extinct species, or cloning for exploitation (i.e. cloning soldiers for warfare). (wikipedia.org)
Conservationists in Indonesia say they've successfully extracted eggs from a Sumatran rhino to be used in an IVF program meant to boost the population of the near-extinct species. (mongabay.com)

The resulting cells were pluripotent and could be differentiated into insulin-producing beta cells to restore the function of the pancreas in the donor. (news-medical.net)
As if it were induced pluripotent stem cells, which really do turn skin into stem cells. (nationalrighttolifenews.org)
Embryonic stem cells are known as pluripotent stem cells. (healthline.com)
These new types of cells are called induced pluripotent stem cells (iPSCs). (healthline.com)
However, some features of these cells are said to be superior to induced pluripotent stem cells, their main rival at this point. (bioedge.org)
Hwang and colleagues report that the cells are chromosomally normal, self-renewing and "pluripotent" - meaning they have the ability to form the three major types of cells in the early embryo that give rise to all other cells in the body. (scienceblog.com)
Embryonic stem cells are pluripotent - they have the ability to become virtually any type of cell within the body. (cbc.ca)
And the other technique, which produces " induced pluripotent stem cells ," skips the step that requires a human egg cell, so some people find it less fraught, ethically. (usf.edu)
These cells are considered pluripotent . (thefutureofthings.com)
In addition, the possibility of reprogramming adult stem cells back to a "pluripotent" (or embryonic-like) state raises the biological prospect of going back too far. (eppc.org)
The greatest hope rests on the potential of pluripotent stem cells, which can become nearly any kind of cell in the body. (pharmaceuticalintelligence.com)
One method of creating pluripotent stem cells is called somatic cell nuclear transfer, and involves taking the nucleus of an adult cell and injecting it into an egg cell from which the nucleus has been removed. (pharmaceuticalintelligence.com)

She holds a Ph.D. in Biological Chemistry and began her career as a laboratory researcher before transitioning to science writing. (news-medical.net)
These cell lines will enable the study of human disease in cells in the laboratory. (scienceblog.com)
In laboratory culture, these cell lines displayed signs of immunological compatibility with the patients' cells, Science authors reported. (scienceblog.com)
Other improvements over the last paper include the reduced use of animal products in laboratory procedures and better evidence that the cell lines matched the patients' cells and did not have a parthenogenetic origin, where unfertilized eggs can divide on their own. (scienceblog.com)
In addition, specific proteins or biological substances can be added to these stem cell cultures to transform them in the laboratory into a large variety of specialized cell types, such as nerve, liver, muscle, bone, and blood cells. (jcpa.org)
In 1995, Campbell and his scientific team used cells grown and differentiated in a laboratory to clone sheep for the first time. (asu.edu)
As with the mice, the team was able to retrieve reproductive cells that produced immature egg cells when grown in the laboratory. (cbc-network.org)
However, most cloning techniques are limited to lab animals that can be reproductively controlled in the laboratory, and thus it is considered difficult to induce reprogramming in wild animals. (mynewsdesk.com)
The close proximity of the laboratory with the expedition sites which will hopefully accelerate the cell obtaining process that is the basis for cloning. (sooam.com)

The researchers wanted to see whether "mature" cells that have differentiated to fulfil a specialised role (such as that of an udder cell or a fetal cell) could be returned to a primitive state from which they could grow into entire organisms. (newscientist.com)
In the field of biotechnology, cloning is the process of creating cloned organisms of cells and of DNA fragments. (wikipedia.org)
Natural cloning occurs through a variety of natural mechanisms, from single-celled organisms to complex multicellular organisms. (wikipedia.org)
Cloning in biotechnology refers to processes used to create copies of DNA fragments ( molecular cloning ), cells (cell cloning), or organisms . (wikiquote.org)
The term applies not only to entire organisms but also to copies of molecules (such as DNA) and cells. (who.int)
Sir John Bertrand Gurdon further developed nuclear transplantation, the technique used to clone organisms and to create stem cells, while working in Britain in the second half of the twentieth century. (asu.edu)

The latest experiments have also produced three lambs from the cells of a sheep fetus aborted after 26 days, and four from a nine-day-old embryo. (newscientist.com)
This Iranian wild sheep was cloned using the same method as Folch et al. (umass.edu)

Next, the researchers take cells containing donor genetic material. (newscientist.com)
This mouse egg (top) is being injected with genetic material from an adult cell to ultimately create an embryo - and, eventually, embryonic stem cells. (usf.edu)
They repeated the process - this time starting with the genetic material extracted from the skin cells of a much older man. (usf.edu)
Most natural cloning occurs in those species that produce their descendants asexually, that is, without combining the male and female genetic material. (who.int)
if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
The DNA within the skin sample is cultured and inserted into a donor egg cell whose nucleus (genetic material) has been removed. (geminigenetics.com)
It involves transplanting the nucleus of one cell, containing an individual's DNA, into an egg cell that has had its genetic material removed. (ohsu.edu)
Furthermore, because these reprogrammed cells can be generated with nuclear genetic material from a patient, there is no concern of transplant rejection," explained Dr. Mitalipov. (ohsu.edu)
Metaphase is a stage in the cell's natural division process (meiosis) when genetic material aligns in the middle of the cell before the cell divides. (ohsu.edu)

Cloning entails taking the nucleus - the compartment that contains the DNA - from an adult cell and putting it into an egg from which the original nucleus has been removed. (nih.gov)

Mammary glands are rich in these cells, which are more adaptable than other tissue. (newscientist.com)
If there are intact cells in this tissue they have been 'stored' frozen. (wikiquote.org)
This means they can potentially produce new cells for any organ or tissue. (healthline.com)
Hwang was the first scientist to publish a paper showing, in 2004, that his team had actually cloned a human cell to provide a source of embryonic stem cells -- master cells that can provide a source of any type of tissue or cell in the body. (blogspot.com)
The process, they said, created stem cells that might be grown into healthy tissue to replace those of people with diabetes, Alzheimer's disease and other disorders. (blogspot.com)
In a study published in the online journal Nature on March 1, 2009, Canadian researches described a new method for generating stem cells from adult human tissue. (cbc.ca)
In a statement on Oct. 31 (which appears to have since been removed), the Indonesian environment ministry said a team of conservation experts extracted the ovarian follicles, which contain the egg, as well as other tissue samples from the rhino at the Kelian Sumatran Rhino Sanctuary (SRS) in East Kalimantan, on the island of Borneo. (mongabay.com)
Adult Stem Cells Taken from Human Fat Tissue Used to Treat Heart Failure Sweden Company Wants To Start First Stem Cell Research Factory Leading Scientist Charges Colleagues With "Misleading" Public British Stem Cell Researcher: Benefits of. (physiciansforlife.org)
Various labs are working together to try and culture mammoth tissue and obtain a viable cell. (sooam.com)
Using two juvenile monkey donors-one expressing GFP and one wild type control-Sun and his team grafted small pieces of testicular tissue onto the backs of castrated male nude mouse recipients. (asianscientist.com)
It may be possible to engineer tissue from embryonic stem cells so doctors don't need to use these drugs. (ox.ac.uk)
It may be possible in the future to embryonic stem cells to produce brain tissue to replace the damaged brain tissue. (ox.ac.uk)

The research breakthrough, led by Shoukhrat Mitalipov, Ph.D. , a senior scientist at ONPRC, follows previous success in transforming monkey skin cells into embryonic stem cells in 2007. (ohsu.edu)

Seoul (AsiaNews) Seoul National University (SNU) yesterday suspended two colleagues of Hwang Woo-suk, a vet and university professor charged with faking his research results on human cloning. (asianews.it)
Lee Byeong-chun and Kang Seung-keun were accused of faking the outcome of studies on cloning human stem cells while working with Hwang. (asianews.it)
Hwang Woo-suk became the first researcher in the world to clone a dog, an Afghan hound named Snuppy, in 2005. (koreatimes.co.kr)
Hwang said his team had created a single cell line from 242 human eggs. (koreatimes.co.kr)
On January 4, 2006, Reuters reported that the journal Science will retract a paper written by disgraced Korean stem cell researcher Hwang Woo-suk and colleagues, accused of faking part of a study on tailored embryonic stem cells, with the retraction announcement coming about one month after Science announced that the only problem was a misunderstanding about photographs. (blogspot.com)
At least one researcher, Robert Lanza, vice president of scientific development at Advanced Cell Technology Inc. in Worcester, Massachusetts, said he asked after the 2004 paper was published that Hwang give samples to another lab to verify. (blogspot.com)
Hwang and 24 co-authors said in the May, 2005 paper that they completed the first transplant of DNA from patients' skin cells into the egg cells of unrelated donors. (blogspot.com)
South Korean professor and cloning pioneer Hwang Woo-suk, is in yet more trouble. (blogspot.com)
hero" is the right word for how Hwang Woo-suk is revered by the media -- and by a large section of the Korean public who have bought into the false promises of embryonic stem cell and cloning researchers. (blogspot.com)
Paul Root Wolpe of the Center for Bioethics at the University of Pennsylvania points out that Hwang did not resign because he used eggs from his researchers but because he lied about it and brought shame "in a country where public shame is so powerful. (blogspot.com)
But what if Hwang Woo-Suk lied about more than the egg donations? (blogspot.com)

Given that we have an efficiency of 1% cloning for livestock species and if only one in a thousand cells are viable then around 100,000 cells would need to be transferred. (wikiquote.org)
In order to accomplish this, the U.S Fish and Wildlife Service and private organizations should provide funds for cloning-based conservation programs to repopulate endangered species that cannot be saved through traditional conservation methods. (umass.edu)
The donor rhino, known as Pahu, is a Bornean specimen of the Sumatran rhino, and her egg would greatly expand the genetic pool of a species believed to number as few as 40. (mongabay.com)
JAKARTA - Conservationists in Indonesia have announced the successful harvesting of egg cells from a female Sumatran rhino, a key step in an effort to breed the critically endangered species in captivity and boost its gene pool. (mongabay.com)
As a Bornean specimen of the Sumatran rhino, Pahu's eggs would greatly enrich the species' gene pool. (mongabay.com)
But in recent years there's been a growing sense of urgency among researchers that the situation is so dire that it's better to focus on preserving the species at all costs rather than trying to maintain two separate subspecies. (mongabay.com)
Previous unsuccessful attempts by several labs showed that human egg cells appear to be more fragile than eggs from other species. (ohsu.edu)

It doesn't create stem cells. (nationalrighttolifenews.org)
However, the process is still ethically controversial, as researchers first create a human embryo and then destroy it to create stem cells. (bioedge.org)

Research advocates attack President Bush for "banning stem cell research," while pro-life advocates lament a Republican administration and Congress that have banned nothing-not embryo destruction, not human cloning, not fetal farming, not genetic engineering. (eppc.org)

Currently, the procedure for isolating non-reproductive cells for the nuclear transfer method involves animal enzymes and serum. (scienceblog.com)
This involves taking some of the contents of a donor cell and injecting it into the egg cell of a woman with infertility problems. (bbc.co.uk)
One technique, maternal spindle transfer (MST), involves transferring the mother's nuclear DNA to an unfertilised donor egg where the nucleus has been removed and then fertilising the egg. (nhmrc.gov.au)
Though those cells have done wonders for science, Henrietta-whose legacy involves the birth of bioethics and the grim history of experimentation on African-Americans-is all but forgotten. (oprah.com)

This allows induction of reprogramming of various animal cell nuclei, which was difficult with conventional cloning methods. (mynewsdesk.com)
The team used cells that were created by transferring the nuclei of adult mouse cells into enucleated eggs cells from genetically different mice. (pharmaceuticalintelligence.com)

Last year they used the same reproductive technology to create the world's first cloned lambs (Nature, vol 380, p 64). (newscientist.com)

The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)

The new technique means they will not need embryonic stem cells. (newscientist.com)
The main difference between the two is that natural cloning does not involve any human intervention, whereas artificial cloning is a genetic engineering technique. (wikipedia.org)
In particular, the efficiency of the process will have to be improved before the technique could be applied in the clinic using human cells. (nih.gov)
So far three attempts by Chinese researchers have made the pitfalls clear: the technique introduces more errors than it fixes . (cosmosmagazine.com)
The other is that co-authors Jin-Soo Kim at Seoul National University in South Korea and Juan Carlos Belmonte at the Salk institute in California - both pioneers of the CRISPR technique - had meticulously optimized the choice of 'guides' for the CRISPR editor by testing them in iPS cell lines that carried the same genetic fault. (cosmosmagazine.com)
However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
A thorough examination of the stem cells derived through this technique demonstrated their ability to convert just like normal embryonic stem cells, into several different cell types, including nerve cells, liver cells and heart cells. (ohsu.edu)

There are also naturally occurring clones among animal populations. (geminigenetics.com)
As well as their distinctive white markings and long curved horns, these cattle are special because they are now considered a herd of naturally occurring clones. (geminigenetics.com)

In bioethics, there are a variety of ethical positions regarding the practice and possibilities of cloning. (wikipedia.org)
The breakthrough may eventually put to rest the ethical controversy surrounding stem cells. (nih.gov)
The HFEA has also announced a public consultation, to run from September until November, to assess the opinion of the British public on the ethical status of egg donation for research. (progress.org.uk)
Adult stem cells don't present any ethical problems. (healthline.com)
Stem cells are at the forefront of medical research and incite some of the most controversial ethical and religious debates worldwide. (thefutureofthings.com)
But adult stem cells also raise some interesting ethical dilemmas alongside their great therapeutic promise. (eppc.org)
While ethical issues about (egg) donation should be debated and the process regulated, the scientific conclusions of Dr. Hwang's research remain intact," Schatten said in a statement. (blogspot.com)
Although we cannot clone a human yet, this experiment occurred almost two years ago and triggered almost an ethical emergency. (benjaminbarber.org)
The ethical and legal controversies that were aroused in the ART debates during the 1980s have been re-ignited with the development of stem cell technology. (edu.au)
This issue was considered by the House of Representatives Standing Committee on Legal and Constitutional Affairs in its report entitled Human Cloning: Scientific, Ethical and Regulatory Aspects of Human Cloning and Stem Cell Research (hereafter the Andrews Report , after the Chair of the Committee, Mr Kevin Andrews, MP) released in September 2001. (edu.au)
The report arose out of a recommendation for the Committee to review the report of the Australian Health Ethics Committee (AHEC) of the NHMRC entitled Scientific, Ethical and Regulatory Considerations Relevant to Cloning of Human Beings (hereafter the AHEC Report ). (edu.au)

Anatomy22

Organisms13

Diseases4

Chemicals and Drugs18

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Psychiatry and Psychology8

Phenomena and Processes39

Disciplines and Occupations14

Anthropology, Education, Sociology and Social Phenomena7

Technology, Industry, Agriculture5

Humanities4

Information Science27

Named Groups6

Health Care27

Geographicals1

Embryos59

Blastocyst13

SCNT28

Organism14

Dolly13

19972

Identical23

Scientists39

Somatic cells3

Regenerative Medicine4

Humans12

Oocytes8

Mitochondrial3

Reproductive cloning of huma5

Scientist Hwang Woo2

20231

Sperm12

Vitro7

Tissues11

Diseases14

Successfully cloned1

Known as reproductive1

Oocyte8

Stem cell lines8

Extinct species3

Pluripotent12

Laboratory9

Organisms6

Sheep2

Genetic material9

Taking the nucleus1

Tissue12

Shoukhrat Mitalipov1

Hwang11

Species7

Create stem cells2

Fetal1

Involves4

Nuclei2

World's1

Adult somatic1

Technique7

Naturally2

Ethical11