Research and clinical applications cell donor human t cells fetal tissue transplantation. Medical search. Frequent questions

The HUCMSCs derived different donors have individual heterogeneity, which potentially lead to distinct therapeutic outcomes in mouse liver fibrosis, indicating we could make use of the donor-variation of MSCs to screen out guaranteed general indicators of MSCs for specific diseases in further stem cell therapy. (researchsquare.com)
Mesenchymal stem cells (MSCs) have been widely tested for treating a variety of refectory medical indications such as type 1 diabetes mellitus, systemic lupus erythematosus, rheumatoid arthritis and Crohn's disease due to their multiple differentiation potentials and immunomodulation capability [1-5]. (researchsquare.com)
MSCs could be derived from a series of tissues including but not limited to umbilical cord, placenta, adipose tissue, bone marrow, gingiva, and dental pulp [5-12]. (researchsquare.com)
These MSCs derived different tissues do not have uniform characteristics, differing in expression profiles of surface markers and biological functions after certain stimulation such as pro-inflammatory mediators [5, 13]. (researchsquare.com)
In spite of tissue origin, many other elements such as culture method and further modulations also influence the heterogeneity of MSCs, leading to differential gene expression profiles, growth phenotype, and differentiation potentials [15-17]. (researchsquare.com)
Over the past few years, MSCs researches have achieved some inspiring results and some of which moved up to clinic period from preclinical phases, resulting in the marketing approval of a few cell-based therapy products (CTPs) by different national regulatory authorities [18]. (researchsquare.com)
We established a Good Manufacturing Practice (GMP) grade cell facility to produce clinic-grade human umbilical cord derived MSCs (HUCMSCs) for treating premature ovarian failure (POF) and recurrent uterine adhesion [18, 20]. (researchsquare.com)
However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
But research progressed and different trials explore the clinical potential of human MSCs isolated from bone marrow but also from other tissues including adipose tissue. (hindawi.com)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
We therefore briefly discuss differences found in subsets of human bmMSCs and in MSCs isolated from some other sources and touch upon how this could be utilized for cell-based therapies. (hindawi.com)
The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
Overview of studies published regarding MSCs using the term "stem cell" or "stromal cell" in the last 20 years accessed by a web search in July 2015 (Google Scholar). (hindawi.com)
It seems that the term "stem cell" became more popular although the "stemness" was only shown in a more strict sense for MSCs involved in osteogenesis and bone repair. (hindawi.com)
When the biological properties of MSCs were explored in more detail, questions arose whether these cells met the criterion of a true stem cell [ 8 ]. (hindawi.com)
Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)
Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors. (nature.com)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
The public clinical trials database http://clinicaltrials.gov shows 123 clinical trials using MSCs for a very wide range of therapeutic applications (Figure 1 ), the majority of which are in Phase I (safety studies), Phase II (proof of concept for efficacy in human patients), or a mixture of PhaseI/II studies (Figure 2 ). (biomedcentral.com)
This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
Adult tissue-derived mesenchymal stromal cells (MSCs) are showing promise in clinical trials for systemic lupus erythematosus (SLE). (nature.com)
Human embryonic stem cell (hESC)-derived MSCs are an alternative to adult MSCs that can circumvent issues regarding scalability and consistent quality due to their derivation from a renewable starting material. (nature.com)
Mechanistically, in vitro data support these findings, as co-culture of hESC-MSCs with lipopolysaccharide (LPS)-stimulated BWF1 lymphocytes decreased lymphocyte secretion of TNFα and IL-6 and enhanced the percentage of putative regulatory T cells. (nature.com)
Evidence shows that MSCs home to sites of inflammation where they inhibit immune and inflammatory responses by influencing the behavior of local innate and adaptive immune cells (reviewed in 20 ). (nature.com)
There is growing evidence that mesenchymal stem cells (MSCs) could change the fate of SCI victims by re-establishing spinal cord function. (anova-irm.com)
Recent research has shown that MSCs also support the re-establishment of blood supply to the damaged spinal cord by inducing angiogenesis. (anova-irm.com)
6,7 Mesenchymal stem cells (MSCs) have also been isolated from other sources, such as bone marrow, 8 the placenta, 9 muscle, 10 or blood. (jcadonline.com)
11 However, the greater the abundance of adipose tissue in the human body, the significantly higher the yield of MSCs is compared to other tissues. (jcadonline.com)
There is compelling evidence that mesenchymal stem cells (MSCs) can be utilized as delivery vehicles for cancer therapeutics. (sscb-stembiotech.com)
More recently, MSCs have been isolated from fetal tissues during gestation or after birth. (sscb-stembiotech.com)
Fetal MSCs derived from amniotic fluid, amniotic membrane, umbilical cord matrix (Wharton's jelly) and umbilical cord blood are more advantageous than adult MSCs, as they can be isolated noninvasively in large numbers without the ethical reservations associated with embryo research. (sscb-stembiotech.com)
Adipose tissue derived mesenchymal stem cells (AT-MSCs) are relatively easy in isolation procedure compared to bone marrow-derived. (ui.ac.id)
This experimental study used cryopreserved adipose tissue derived MSCs stored in Stem Cell Medical Technology Integrated Service Unit Cipto Mangunkusumo Hospital. (ui.ac.id)
BACKGROUND AND AIMS: Mesenchymal stromal cells (MSCs) a potentially effective disease-modulating therapy for diabetic nephropathy (DN) but their clinical translation has been hampered by incomplete understanding of the optimal timing of administration and in vivo mechanisms of action. (bvsalud.org)
This study aimed to elucidate the reno-protective potency and associated mechanisms of single intravenous injections of human umbilical cord-derived MSCs (hUC-MSCs) following shorter and longer durations of diabetes. (bvsalud.org)
In groups of diabetic animals, human (h)UC-MSCs or vehicle were injected intravenously at 8 or 16 weeks after STZ along with vehicle-injected non-diabetic animals. (bvsalud.org)
Bio-distribution of hUC-MSCs in kidney and other organs was evaluated in diabetic mice by injection of fluorescent-labelled cells. (bvsalud.org)
Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
The final aim of this mini review is to illustrate the heterogeneity of current strategies for gaining MSCs for clinical applications with their strengths and weaknesses. (frontiersin.org)
Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
In this mini review, we propose a hexagon of steps to consider during selection, pretreatment, analysis, and application of MSCs in order to improve the transferability of promising preclinical results into clinical success ( Figure 1 ). (frontiersin.org)
Hexagon toward translating MSCs' promise into clinical reality. (frontiersin.org)
Besides stimulating ECM production, IGF-1 can stimulate the proliferation and chondrogenic differentiation of mesenchymal stem cells (MSCs) [ 7 ]. (biomedcentral.com)

[5] Conditions such as anemias and immunodeficiencies, for which fetal tissue attempts largely failed, are now treated routinely with adult stem cells, including umbilical cord blood stem cells, [6] even while the patient is still in the womb. (lozierinstitute.org)
Recent medical advances in the field of regenerative medicine and tissue transplantation have highlighted the importance of umbilical cord blood (UCB) as a valuable alternative source of haematopoietic stem cells, which are potentially life-saving in a vast array of clinical applications. (sajbl.org.za)
Salvador, a five-year-old Portuguese boy who was diagnosed with autism spectrum disorder, recently underwent treatment using stem cells from his own umbilical cord blood with the aim of improving his condition. (sscb-stembiotech.com)

One patient who received transplant of fetal brain tissue (from a total of 3 fetuses) died subsequently, and at autopsy was found to have various non-brain tissues ( e.g, skin-like tissue, hair, cartilage, and other tissue nodules) growing in his brain. (lozierinstitute.org)
Mesenchyme directly gives rise to most of the body's connective tissues, from bones and cartilage to the lymphatic and circulatory systems. (asu.edu)
Currently, the only FDA-approved cell-based therapy for cartilage defects involves autologous chondrocyte implantation: chondrocytes harvested from low-contact areas are expanded in vitro and then re-injected directly into the damaged site 1 . (nature.com)
Accordingly, investigations on cellular therapies have therefore moved to progenitor cell populations such as bone marrow derived mesenchymal stem cells (BMSCs), which have the ability to differentiate into cartilage cells 4 . (nature.com)
Besides the cell source choice, also mechanical stimuli are crucial in the development and maintenance of articular cartilage. (nature.com)
These studies have demonstrated that mechanical forces are able to shape the mesenchymal stem cell (MSC) fate when appropriately applied in in vitro models of cartilage regeneration. (nature.com)
Adult stem cells can be used to accelerate bone or tendon healing , and they can induce cartilage progenitor cells to produce a better matrix and repair cartilage damage . (thefutureofthings.com)
Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in Steep. (sciendo.com)
Introduction to anatomy, general features of epithelial tissue, general organization of connective tissue, development of and hstology of cartilage and bone tissues, embryology and histology of skin, biochemistry of connective tissue, and bone tissue, skin and tissue parasites and fungi, arthropods and arthropods borne viruses. (atilim.edu.tr)
Isolation and cultivation of mesenchymal stem cells from iliac crest bone marrow for further cartilage defect management. (ui.ac.id)
Optimization of IGF delivery systems will facilitate treatment application in cartilage repair and improve OA treatment efficacy. (biomedcentral.com)
Thus, chondrocyte metabolism in the adjacent cartilage is relatively low, and these cells cannot easily migrate to the damaged site [ 2 ]. (biomedcentral.com)
However, clinical efficacy of these treatments is not conclusive, as these treatments do not consistently regenerate hyaline cartilage. (biomedcentral.com)
Strategies for IGF delivery to chondrocytes and cartilage matrix are essential for its clinical application in OA treatment. (biomedcentral.com)
An ex vivo study showed that IGF-1 in fetal bovine serum was responsible for maintaining articular cartilage proteoglycan synthesis. (biomedcentral.com)
Трансплантати шкіри Many different types of tissues can be transplanted, including skin, bone, cartilage, adrenal tissue, fetal thymus, corneas, and composite transplantation of tissues to restore the face or extremities. (msdmanuals.com)

Israeli policy is based on the belief that such a pre-embyro does not confer personhood and that many therapeutic applications can be derived from such research. (jcpa.org)
In view of the fact that UCB has become a valuable, non-invasive source of stem cells for regenerative therapy, establishment of a public cord blood bank (CBB) in South Africa would vastly improve the availability of haematopoietic stem cells for research and therapeutic uses, and increase the tissue genetic diversity that currently impedes the South African bone marrow registry. (sajbl.org.za)
As a source of non-embryonic stem cells, UCB has found potential uses as a therapeutic modality in more than 80 clinical applications, both haematological and non-haematological. (sajbl.org.za)
University researchers ("researchers") may conduct research on the transplantation of human fetal tissue or cell lines derived from human fetal tissue ("human fetal tissue") for therapeutic purposes only in accordance with applicable federal and state laws and regulations and University policies and procedures. (umn.edu)
To compare the heterogeneities of human umbilical cord mesenchymal stem cells (HUCMSCs) derived from different donors and test their therapeutic variations in mouse liver fibrosis model. (researchsquare.com)
Expectations for patient benefits are high in these therapeutic applications. (biomedcentral.com)
In this review, we describe the cardiac differentiation from ES cells, iPS cells, and the current progress of using iPS cell-derived cardiomyocytes for heart disease modeling and for the development of therapeutic strategies. (biomedcentral.com)
A number of large biotech companies and scientists are looking toward stem cells as the basis for a therapeutic solution to cure such illnesses as blindness, diabetes and spinal cord injuries. (thefutureofthings.com)
In particular, due to the limited regenerative capacity of the adult nervous system, the therapeutic potential of stem cells has opened up new perspectives for development of treatment strategies for brain repair after injury or disease. (ehu.eus)
Therefore, the therapeutic use of appropriate populations derived from stem and progenitor cells has been considered for cell-based therapies for neurodegenerative diseases, primarily Parkinson's disease, and brain injuries. (ehu.eus)
CD20-transduced T cells with and without co-transferred T-cell receptor were efficiently eliminated by complement dependent cytotoxicity induced by therapeutic anti-CD20 antibody rituximab. (haematologica.org)
Finally, and inexorably, a true professional scientist poses clearly challenging questions to his research colleagues, and to the scientific enterprise in general, about the dubious "scientific" justification for the current rush to clone human beings - for both "therapeutic" and for "reproductive" purposes. (lifeissues.net)
But he is equally concerned about the unethical aspects inherent in the rush to perform " therapeutic " human cloning research, including the abuses to all vulnerable human patients who would be required to participate in clinical trials. (lifeissues.net)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
Stem cell therapy provides hope for many patients, but should be tempered with the realisation that the scientific and medical communities are still to fully unravel the complexities of stem cell biology, and to provide satisfactory data that support the rational, evidence-based application of these cells from a therapeutic perspective. (sscb-stembiotech.com)
The purpose of this study is to examine the therapeutic efficacy of human umbilical cord mesenchymal stem/stromal cells (UMSCs) in treating congenital and acquired corneal opacity associated with the loss of collagen V. (molvis.org)
Mesenchymal stem cells: immunology and therapeutic benefits. (ui.ac.id)
2011. http://www.intechopen.com/books/stem-cells-in-clinic-and-research/mesenchymal-stem-cells-immunology-and-therapeutic-benefits . (ui.ac.id)

Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
In this review, we discuss the potential influence of stromal vascular fraction from adipose tissue in the management of alopecia as well as its involvement in preclinical and clinical trials. (jcadonline.com)
however, a variety of other cell types are found as well, collectively labeled as stromal vascular fraction (SVF). (jcadonline.com)
Defining essential stem cell characteristics in adipose-derived stromal cells extracted from distinct anatomical sites. (ui.ac.id)

Stem cell research is, in part, a quest to understand cellular differentiation, the process by which a human being develops from one fertilized cell into a multicellular organism composed of over 200 different cell types - for example muscle, nerve, blood cell, or kidney. (jcpa.org)
Cellular differentiation begins with the fertilized egg which serves as the identifying characteristic of an embryonic stem cell. (jcpa.org)
UCB's advantages over other sources of stem cells such as adult bone marrow and embryonic stem cells include easy procurement, minimal risk to the donor, excellent proliferation and differentiation, immediate availability, and autologous use. (sajbl.org.za)
The HUCMSCs derived multiple donors were performed comprehensively analysis and potent assays including expressions of surface markers, viability, growth curve, karyotype analysis, tumorigenicity, differentiation potentials, and immune regulation capability. (researchsquare.com)
The HUCMSCs derived multiple donors kept a high consistency in surface marker expressions, viability, growth curve, tumorigenicity in nude mic, but had robust heterogeneities in differentiation potentials and immune regulations. (researchsquare.com)
They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
The term stem cell can be defined by two very important qualities: the cell has the ability to self-renew and, in a more general sense, the cell has not completed differentiation into its final state. (thefutureofthings.com)
This general definition includes a wide variety of cells with varying degrees of differentiation potential. (thefutureofthings.com)
2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
METHODS: Cultured isolated cells from gingival tissues of 4 healthy guinea pigs at passage 3 were characterized as GMSCSs using flow cytometry for surface markers and multilineage differentiation capacity. (bvsalud.org)

The widespread success of foetal tissue transplantation led to the use of foetal brain cells to treat neurological diseases. (wikipedia.org)
While there is a great deal published on the potential medical applications of stem cell research to treat or cure diseases such as diabetes, Alzheimer's disease, Parkinson's disease, cancer, and heart disease, much less has been published on the future impact of stem cell research in reproductive medicine. (jcpa.org)
For the first 10 years of my research career at Canadian Blood Services, we were involved in developing new ways to identify blood groups and test for infectious diseases. (blood.ca)
Organ transplantation is the ultimate treatment option for patients suffering from refractory diseases. (frontiersin.org)
When germ-free cell cultures became a laboratory routine, hopes were high for using this novel technology for treatment of diseases or replacement of cells in patients suffering from injury, inflammation, or cancer or even refreshing cells in the elderly. (hindawi.com)
More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
Both ES cells and iPS cells are pluripotent stem cells with capabilities of indefinite self-renewal and can be differentiated into almost all cell types of the body, which make them valuable for studying early developmental biology, for modeling and as therapy for human diseases. (biomedcentral.com)
They can thus develop into brain, muscle, blood, skin, and other tissues and can, in theory, help with many health conditions involving organ dysfunction or failure, as well as cancer, injury, and may even address degenerative and otherwise incurable diseases such as Parkinson's and Alzheimer's disease. (nhsjs.com)
Kavita Sharma, Syed Yawer Husain, Pragnya Das, Mohammad Hussain, and Mansoor Ali Syed Despite substantial clinical advances over the past decades, inflammatory lung diseases are a major cause of morbidity and mortality and have become one of the major public health issues across the world. (ebrary.net)
Initially, two‐dimensional neural cultures presented an innovative landmark for investigating human neuronal and, more recently, glial biology, as well as for modeling brain neurodevelopmental and neurodegenerative diseases. (ehu.eus)
OMIM [3], what I call "Wikipedia for Smart Medical Students," is essential when considering any and all human genetic diseases. (nakedcapitalism.com)
The study was published online Sept. 21, 2023 , in the journal Science by a research team led by Sing Sing Way, MD, PhD, Division of Infectious Diseases at Cincinnati Children's and the Center for Inflammation and Tolerance. (fox59.com)
Claims that you could clone individual treatments of human beings to treat common diseases like diabetes, suggests you need a huge supply of human eggs. (wikiquote.org)

However, the success of fetal tissue transplants has been meager at best, and ethically-derived alternatives exist and are coming to dominate the field. (lozierinstitute.org)
The first recorded fetal tissue transplants were in 1921 in the UK, in a failed attempt to treat Addison's disease, [1] and in 1928 in Italy, in a failed attempt to treat cancer. (lozierinstitute.org)
That attempt also failed, as did subsequent similar fetal tissue transplants in 1959. (lozierinstitute.org)
Between 1970 and 1991 approximately 1,500 people received fetal pancreatic tissue transplants in attempts to treat diabetes, mostly in the former Soviet Union and the People's Republic of China. (lozierinstitute.org)
[3] Today, patients take insulin shots and pharmaceuticals to control their diabetes, and adult stem cell transplants have shown success at ameliorating the condition. (lozierinstitute.org)
[8] Subsequent reports showed that severe problems developed from fetal tissue transplants. (lozierinstitute.org)
A second large, controlled study published in 2003 showed similar results (funded by NIH), with over half of the patients developing potentially disabling tremors caused by the fetal brain tissue transplants. (lozierinstitute.org)
[12] The results of these two large studies led to a moratorium on fetal tissue transplants for Parkinson's. (lozierinstitute.org)
[ 1 ] More than half of autologous transplantations are performed for multiple myeloma and non-Hodgkin lymphoma , and the vast majority of allogeneic transplants are performed for hematologic and lymphoid cancers. (medscape.com)
That is typically true in cases of heart or lung transplantation, but not in the case of bone marrow transplants or even kidney transplantation, he said. (genomeweb.com)
Over the years, there have been attempts to cure diabetes by using methods like replacing b-cells through transplants, by increasing b-cell replication, by reducing the death of b-cells and by deriving new b-cells from other cells. (livestemcells.asia)
By the end of the twentieth century, many other transplants using different processes had been reported but there were challenges like the lack of appropriate donor tissue and use of immune-suppressive drugs to prevent rejection of the transplant. (livestemcells.asia)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
Living donors are often used for kidney and hematopoietic stem cell transplants, less frequently for segmental liver transplants, and rarely for pancreas and lung transplants. (msdmanuals.com)

Foetal tissues have also been used in liver and thymus transplantations (1968). (wikipedia.org)
Between 1960 and 1990, numerous attempts were made to transplant fetal liver and thymus for various conditions. (lozierinstitute.org)

Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
In conclusion, gene-corrected hematopoietic stem cell transplantation has promising outcomes for SCD, Fanconi anemia, and thalassemia, and it may overcome the limitation of the source of allogenic bone marrow transplantation. (sscb-stembiotech.com)

Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
EBV-transformed B cells (EBV-LCL) were cultured in IMDM (Cambrex, Verviers, Belgium) with 10% Fetal Bovine Serum (FBS). (haematologica.org)

Our hospital is approved to be qualified for implementing stem cell clinic trials by government agencies [19]. (researchsquare.com)
In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
At the same time, the first three trials using cells derived from pluripotent cells have begun. (biomedcentral.com)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
Several of those are now considered applicable for patient treatments beyond the need for regulated clinical trials. (biomedcentral.com)
We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
In rodents, and even in some preliminary trials in humans, human embryonic stem cells have been shown to bridge gaps in spinal cord injuries , allowing restoration of motor functions. (thefutureofthings.com)
The objective of this report is to review the most recent clinical trials involving blood and BMT for SCD and to discuss novel approaches to overcome the many barriers to successful use of BMT for SCD. (johnshopkins.edu)
15 Several second generation anti-CD20 antibodies have recently been developed, and clinical trials are currently ongoing to investigate their in vivo efficacy. (haematologica.org)
Ethically, since eventually all such "research" will be applied to people, he cautions against the abuse of women "egg" donors, and against the premature use of vulnerable sick human patients for testing supposedly "patient-specific" stem cells in supposed "therapies", pointing to the obvious violations of standard international research ethics guidelines such clinical trials would necessarily entail. (lifeissues.net)
As he has questioned the HFEA before, would not the use of vulnerable human patients in clinical trials be premature, dangerous, and unethical given the already acquired knowledge in the research community that such supposed "patient-specific" stem cells would most probably cause serious immune rejection reactions in these patients? (lifeissues.net)
Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
Clinical trials have demonstrated that CRT can provide long-lasting, significant clinical benefit. (lu.se)

In 2009, in a major reversal of U.S. policy, President Obama signed an executive order pledging to "vigorously support" embryonic stem cell research. (jcpa.org)
While regarded by many top scientists as the Holy Grail of medicine, others consider embryonic stem-cell research sacrilegious. (thefutureofthings.com)
Considering the great potential of embryonic stem cell research, it is argued here that their research be allowed to be legal, federally funded, and its development a national priority. (nhsjs.com)
The controversy over embryonic stem cell research is caused by the fact that the procurement of these stem cells involves the destruction of the embryo produced during in vitro fertilization. (nhsjs.com)
In the United States currently embryonic stem cell research is allowed but there has been a lot of public controversy and legal setbacks. (nhsjs.com)
This newer bill calls for prioritizing federally assisted advancement of embryonic stem cell research ( 1). (nhsjs.com)
Additionally, there have been two executive orders focusing on embryonic stem cells, one released by President George W. Bush prohibiting embryonic stem cell research and related federal funding, the other by President Barack Obama reversing the previous order but still with restrictions in place ( 2). (nhsjs.com)
Experts from around the world are assessing the difficult issue of the extent to which embryonic stem cell research should be allowed to proceed, and to date there is little international consensus on this matter. (edu.au)
How, then, should embryonic stem cell research be regulated in Australia? (edu.au)
In this article we examine embryonic stem cell research and explore the current regulatory framework associated with this research in Australia, with particular reference to the Andrews Report . (edu.au)

In order to better appreciate the role of stem cell research in reproductive medicine, there is a need to understand the critical biological principles of stem cell research and its potential applications to medicine. (jcpa.org)
Recent advances in the field of stem-cell research are giving hope to millions. (thefutureofthings.com)
A particular field encouraged by the foundation is stem-cell research, with the great hope that it will result in the ability to get cells to differentiate into neurons and support cells to bridge the gap of a spinal cord injury. (thefutureofthings.com)
Stem cell research represents one of the most polarized biomedical controversies of our time. (nhsjs.com)
Proponents of stem cell research claim that the blastocyst is not human yet, and the embryos used for stem cell harvest are typically leftover from in vitro fertilization procedures with minimal chance that a human could ever develop from them. (nhsjs.com)
Adversaries of stem cell research argue that embryos are human and destroying one is equal to murdering a child. (nhsjs.com)
Stem cell research is one of the most controversial issues in modern medicine. (nhsjs.com)
Two bills were proposed: The first one was the Stem Cell Research Advancement Act, which passed in both the House of Representatives and the Senate but was vetoed by President George W. Bush. (nhsjs.com)
This showcases the ambivalence of public perception, policy and legislation about stem cell research. (nhsjs.com)
This issue was considered by the House of Representatives Standing Committee on Legal and Constitutional Affairs in its report entitled Human Cloning: Scientific, Ethical and Regulatory Aspects of Human Cloning and Stem Cell Research (hereafter the Andrews Report , after the Chair of the Committee, Mr Kevin Andrews, MP) released in September 2001. (edu.au)
Great Iranian Muslim scholars netics, stem cell research, and organ trans- laid huge emphasis on teaching and practis- plantation are some of the medical issues ing ethics. (who.int)

Deficiency or malfunction of these blood cells occurs in disease conditions prevalent in Africa, including cancers like leukaemia and haemoglobinopathies like thalassaemia or sickle-cell disease. (sajbl.org.za)
Bolaños-Meade, J & Brodsky, RA 2009, ' Blood and marrow transplantation for sickle cell disease: Overcoming barriers to success ', Current opinion in oncology , vol. 21, no. 2, pp. 158-161. (johnshopkins.edu)
Hereditary anemia has various manifestations, such as sickle cell disease (SCD), Fanconi anemia, glucose-6-phosphate dehydrogenase deficiency (G6PDD), and thalassemia. (sscb-stembiotech.com)

Lastly, the cells can survive in lower oxygen conditions, and tend to be more resistant to ischemic environments during transplantation or in vitro conditions. (wikipedia.org)
The collected cells first undergo in vitro culture to check for any gene abnormalities and cell dysfunctions, then the cells will be injected into the patient through implantation surgeries. (wikipedia.org)
The harvesting of cells from foetuses are done according to the standardised stem cell protocol, this includes the following: Place isolated cells on ice Calculate cell viability Culture cells to transform into a suspension Grind to dissolve cells Number and size of clones increases over time After a week of culturing, around 40 to 50% of the cells do not survive in vitro. (wikipedia.org)
Mouse ES cells have been widely utilized as an in vitro model to study cardiogenesis, as cardiomyocytes were found to spontaneously differentiate from ES cells after withdrawal of LIF (leukemia inhibitory factor), which functions to maintain the pluripotency of undifferentiated mouse ES cells [ 2 - 4 ]. (biomedcentral.com)
The source of embryos is from those fertilized in vitro , and then donated for research with donor consent. (nhsjs.com)
Here we highlight the journey towards recapitulating human brain development and disease in a dish, progressing from two‐dimensional in vitro systems to the third dimension provided by brain organoids. (ehu.eus)
T-cell receptor (TCR) gene transfer is an attractive strategy for rapid in vitro generation of high numbers of antigen specific T cells. (haematologica.org)
HADSCs were induced to confirm that they can express the characteristics of endothelial cells (ECs) in vitro. (molvis.org)
Therefore, in this study, we assessed the ability of human adipose mesenchymal stem cells (hADSCs) to express the characteristics of ECs after induction in vitro to evaluate the theoretical feasibility of maintaining or restoring the function of ECs after intraocular application. (molvis.org)
In this thesis, human glia-to-neuron direct conversion and engineered viral vectors are explored using pre-clinical in vitro and ex vivo models. (lu.se)
In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)

The predominant bioethical concern arising from this technology is that the blastocyt-stage embryo must be destroyed in the process of isolating and separating the embryonic stem cells from the inner mass region of the pre-embryo. (jcpa.org)
The destruction of the pre-embryo has been the critical issue in the U.S. behind imposing limits on federal government-sponsored research in embryonic stem cells. (jcpa.org)
ABP also may accept donations of human fetal tissue obtained from a stillborn infant, or an embryo or fetus that died of natural causes in utero as authorized under applicable state laws. (umn.edu)
Teratogens are substances that may produce physical or functional defects in the human embryo or fetus after the pregnant woman is exposed to the substance. (asu.edu)
The development of the obstetric ultrasound has allowed physicians and embryologists to obtain a clear picture of the developing human embryo and fetus during pregnancy. (asu.edu)
The early mammalian embryo consists of the extra-embryonic cell layers-the trophoblast and a body of cells called the inner cell mass (ICM), which eventually become the embryo proper. (thefutureofthings.com)
However, the removal of embryonic stem cells destroys the early embryo. (nhsjs.com)
It is also our view that there are no sound reasons for treating the early-stage human embryo or cloned human embryo as anything special, or as having moral status greater than human somatic cells in tissue culture. (wikiquote.org)
Assisted reproductive technology (ART) and embryo research have posed many challenges to the different timeframes of science, ethics and law. (edu.au)

Derived from the fetal allantois, it provides a rich source of multipotent stem cells, including CD34+ and CD38- haematopoietic progenitor cells. (sajbl.org.za)
These progenitor cells have greater clonal expansion and proliferative capacity than normal bone marrow cells. (sajbl.org.za)
At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)
Intradermal adipocyte progenitor cells are activated toward the end of the telogen phase to proliferate and differentiate into mature adipocytes. (jcadonline.com)

Our general approach involves examining the natural world to learn how plants and animals survive extreme environmental stress and then assessing the application of these adaptations for clinical medicine. (blood.ca)
Cell therapy involves cultivating or modifying immune cells outside the body before injecting them into the patient. (ajmc.com)
If the proposed research involves aborted fetal tissue, the application to FTR will include a written explanation of the need for human fetal tissue from induced abortions. (umn.edu)
A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
As genetic aberrations are the main causes of all forms of hereditary anemia, the optimal approach involves repairing the defective gene, possibly through the transplantation of normal hematopoietic stem cells (HSCs) from a normal matching donor or through gene therapy approaches (either in vivo or ex vivo) to correct the patient's HSCs. (sscb-stembiotech.com)
This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)

This has enabled us to improve methods for storing cells and tissues for transfusion and transplantation. (blood.ca)
This, along with the development of unrelated cord blood transplantation and familial haploidentical transplantation methods, have improved the likelihood of finding an appropriate HSCT source in a timely manner. (medscape.com)
Building off previous methods that have demonstrated that shotgun sequencing of cell-free DNA shed in the bloodstream from the donor organ can identify rejection, the team demonstrated that the donor does not first have to be genotyped, which they said would make it more useful in a clinical setting. (genomeweb.com)
The group described the method this month in PLOS Computational Biology and plans to work with the National Institutes of Health's Genome Research Alliance for Transplantation consortium to test their approach alongside other methods, including the gold standard of invasive biopsy. (genomeweb.com)
METHODS: Ten MZ twin pairs requested ovarian transplantation and eight have undergone transplantation with cryopreservation of spare tissue. (infertile.com)
Then, hADSCs were intraocularly injected into a pathological model (oxygen-induced retinopathy [OIR] model) to check the movement trajectory, fusion, proliferation, and prognosis of the hADSCs in the intraocular application using morphological methods. (molvis.org)
Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. (frontiersin.org)

Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)
Worldwide, approximately 90,000 first HSCTs-53% autologous and 47% allogeneic-are performed every year, according to the World Wide Network of Blood and Marrow Transplantation. (medscape.com)
Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
Herpes simplex virus thymidine kinase (HSV-tk) is a well-established suicide gene that has been successfully used to control GvHD following donor lymphocyte infusions (DLI) after allogeneic stem cell transplantation. (haematologica.org)
4 In addition, TCRs for minor histocompatibility antigens, including HA-1 and HA-2, have been transferred to donor-derived virus-specific T cells to treat patients with hematologic malignancies after allogeneic stem cell transplantation. (haematologica.org)
T-cell clones were stimulated every 10-14 days with irradiated allogeneic PBMC and phyto-hemagglutinin in IMDM with 5% human serum (HS), 5% FBS and 100 IU/mL IL-2 as previously described. (haematologica.org)

Foetal cells are differentiated stem cells from pluripotent embryonic stem cells. (wikipedia.org)
Embryonic stem (ES) cells are naturally derived from early stage embryos and induced pluripotent stem (iPS) cells are reprogrammed from somatic cells with overexpression of four reprogramming factors, Oct4, Sox2, Klf4 and c-Myc. (biomedcentral.com)
These cells are considered pluripotent . (thefutureofthings.com)
ES are pluripotent, as they can transform into almost any human cell type. (nhsjs.com)
There are other, less controversial alternatives to embryonic stem cells, such as adult, fetal, cord blood, and induced pluripotent stem cells, but they have other biological restrictions that make them less promising for use in regenerative medicine at this time. (nhsjs.com)
In particular, the advent of human embryonic stem cells followed by reprograming technologies for generation of induced pluripotent stem cells have instigated studies into modeling human brain development and disease by providing a means to simulate a human tissue otherwise completely or largely inaccessible to researchers. (ehu.eus)
This led us to question whether excessive Ca V 3 channels, retained mistakenly in engineered human-induced pluripotent stem cell-derived islet (hiPSC-islet) cells, act as an obstacle to hiPSC-islet maturation. (mdpi.com)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
Moreover, we expound the future research direction of HSC derivation from induced pluripotent stem cells (iPSCs), strategies to edit HSCs, gene therapy risk mitigation, and their clinical perspectives. (sscb-stembiotech.com)
Cell therapies derived from differentiated embryonic stem cells and induced pluripotent stem cells are also starting to feature prominently. (sscb-stembiotech.com)
No evidence of tumour formation was observed in the transplanted animals, a major concern when utilising a pluripotent cell source. (lu.se)

Due to the presence of low levels of histocompatibility antigens in foetal cells, the chances of the recipient rejecting the transplanted foetal cell is very low. (wikipedia.org)
We are contributing to national studies linking data about donors, products and recipient outcomes. (blood.ca)
Researchers may accept and/or use human fetal tissue for transplantation into a relative of the donor or other individual designated by the donor (i.e., donor-designated recipient) only if the tissue is obtained from a spontaneous abortion or stillbirth. (umn.edu)
That previous work, in both heart and lung transplantation, relied on genotyping both the organ donor and the recipient in order to distinguish which cfDNA fragments were being shed from the donor organ, and which were naturally occurring from the recipient. (genomeweb.com)
The idea was to find the most likely proportion of cfDNA that is donor derived given the recipient genotype and cfDNA sequences by iterating over different donor ancestral populations and accounting for sequencing and genotyping errors," Sharon said. (genomeweb.com)
One key factor though, Sharon said, is that the model assumes that the organ donor and the recipient are not related. (genomeweb.com)
For those cases, we took another step and modeled the relationship between the donor and the recipient and calculated for that," Sharon said. (genomeweb.com)
The statistical models help estimate the proportion of cfDNA fragments that are from the recipient and donor. (genomeweb.com)
The researchers compared their so-called one-genome model to the two-genome method, where both donor and recipient genotypes are known. (genomeweb.com)
They found that the statistical model was able to learn the relationship between the donor and recipient and that the one-genome method was comparable to the two-genome method. (genomeweb.com)
1 , 2 The anti-tumor effect of DLI is mediated by donor T cells recognizing allo-antigens on the malignant cells of the recipient. (haematologica.org)
Probable contamination - The blood culture from the recipient is negative or could not be done, but there is definite bacterial growth in the donor blood product. (medscape.com)

These preparatory interregional and interdisciplinary meetings focused on the following areas: cloning and human reproductive health, biologicals, organ transplantation, research, and medical genetics. (who.int)
The ideal treatment option for terminally ill patients is organ transplantation. (frontiersin.org)
However, donor organs are in absolute shortage, and sadly, most patients die while waiting for a donor organ. (frontiersin.org)
In this regard, emerging technologies of chimeric human organ production via blastocyst complementation (BC) holds great promise. (frontiersin.org)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
Presently, in the United States, another person is added to an organ transplant list every 10 min, 17 people die each day while waiting for donor organs, and approximately 105,800 patients are waitlisted for an organ transplant according to the health resources and services administration (HRSA). (frontiersin.org)
Depending on the patient's medical condition, a refractory disease patient also requires an on-time selective option, such as less invasive cellular therapy options or curative organ transplantation that can function immediately after transplantation. (frontiersin.org)
However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
In this review, we summarize the history of interspecies chimerism in various animal models to find hints for BC application and describe the challenges and prospects of utilizing BC for human organ generation. (frontiersin.org)
After his PhD examination, his biochemical studies relating to ABO-incompatible organ transplantation continued in the Department of Surgery. (gu.se)
Implications could extend into vaccine research and organ transplantation. (fox59.com)
In addition to potentially making progress against the leading cause of infant mortality, Way says understanding how the immune system changes during pregnancy could influence other research fields including vaccine development, autoimmunity research, and how to prevent organ transplant rejection. (fox59.com)
Even many years later, these cells help explain why an organ transplant from a person's mother is more likely to be successful compared to a donor organ from their father. (fox59.com)
Трансплантація печінки Liver transplantation is the 2nd most common type of solid organ transplantation. (msdmanuals.com)
Almost always, transplantation is done to replace or restore the function of an end-stage diseased organ, thereby restoring an essential function and improving patient survival. (msdmanuals.com)

These results have been applied in clinical practice to establish ABO-incompatible renal and liver transplantation programs in Gothenburg and Birmingham, UK. (gu.se)
Indications for liver transplantation include Cirrhosis (70% of transplantations. (msdmanuals.com)

ES cells were aggregated into three-dimensional structures, termed embryoid bodies (EBs), and suspended in media containing fetal calf serum. (biomedcentral.com)

However, the worldwide shortages of corneal donor material generate a strong demand for personalized stem cell-based alternative therapies. (lww.com)
Although only a handful of cell and gene therapies are on the market, FDA officials predicted in 2019 that the agency will receive more than 200 investigational new drug applications per year for cell and gene therapies, and that by 2025, it expects to have accelerated to 10 to 20 cell and gene therapy approvals per year. (ajmc.com)
In total, 3 CAR T-cell therapies are now on the market, all targeting the CD19 antigen. (ajmc.com)
Considerable time and effort has been spent in developing targeted therapies to fight SLE, yet only one therapy, belimumab (Benlysta), a monoclonal antibody targeting B cell-activating factor, or BAFF, has been approved for the treatment of SLE in the last half-century 16 . (nature.com)
The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)
Alternative therapies, including autologous chondrocyte implantation (ACI), matrix-induced ACI (MACI), and stem cell transplantation, are also available commercially or are in the clinical study phase. (biomedcentral.com)

Essentially, you can kill any cancer cell that has an antigen that is recognized by the immune cell," Hwu said. (ajmc.com)
During more recent years, his reseach has been conducted in the field of regenerative medicine, focussed on antigen expression and immune recognition of human embryonic stem cells and bioartificial heart valves, the latter tissues being of animal origin. (gu.se)
Unfortunately, most patients with SCD do not have a suitable human lymphocyte antigen-matched sibling donor. (johnshopkins.edu)
Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. (thermofisher.com)
The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. (thermofisher.com)
The use of CD20 as suicide gene was investigated in CMV specific T cells and in T cells genetically modified with an antigen specific T-cell receptor. (haematologica.org)
Although human CD20 has previously been described as functional selection marker and suicide gene after retroviral transfer to T lymphocytes, 9 , 10 the specificities of these T cells were unknown, and therefore a potential effect of CD20 on antigen-specific T-cell functions could not be investigated. (haematologica.org)
CD11a/CD18 (LFA-1) expressed on lymphocytes is known to play an important role in lymphocyte trafficking (adhesion to vascular endothelium), as well as interactions to antigen presenting cells (APC). (medscape.com)

After lots of trial and error, bone marrow transplantation became successful in the 1970s, where there were no infectious complications or transplant rejection. (wikipedia.org)
Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)

Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
Treatment of complete spinal cord injury patients by autologous bone marrow cell transplantation and administration of granulocyte-macrophage colony stimulating factor. (ui.ac.id)

Side-by-side comparison of the biological characteristics of human umbilical cord and adipose tissue-derived mesenchymal stem cells. (ui.ac.id)
Prospect of adipose tissue derived mesenchymal stem cells in regenerative medicine. (ui.ac.id)

Corneal transplantation is currently the only therapy for severe corneal disorders. (lww.com)
The current radical treatment of severe HF is cardiac transplantation. (ebrary.net)
Adverse side effects, however, may occur and lead to severe autoimmunity or graft-versus-host disease (GvHD), emphasizing the relevance of a suicide mechanism that allows efficient in vivo elimination of infused T cells. (haematologica.org)
Transfer of HSV-tk to DLI preserves the beneficial anti-tumor effect and allows in vivo elimination of donor T cells if severe GvHD occurs. (haematologica.org)
Трансплантація рогівки Corneal transplantations are done for several reasons: To reconstruct the cornea (eg, replacing a perforated cornea) To relieve intractable pain (eg, severe foreign body sensation due to recurrent. (msdmanuals.com)

Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. (lww.com)
1 If the number of corneal endothelial cells (CECs) falls below a certain threshold because of traumatic injury, disease, or normal aging processes, functionality of the CE is decreased, and the cornea swells and becomes milky, leading to eventual vision loss. (lww.com)
The corneal stroma is composed of primarily collagen fibrils, small leucine-rich proteoglycans (SLRPs), as well as sparsely distributed cells called keratocytes. (molvis.org)
Experimental mice, i.e., wild-type, Col5a1 f/f and Kera-Cre/Col5a1 f/f ( Col5a1 ∆st/∆st , collagen V null in the corneal stroma) mice in a C57BL/6J genetic background, were subjected to a lamellar keratectomy, and treated with or without UMSC (10 4 cells/cornea) transplantation via an intrastromal injection or a fibrin plug. (molvis.org)
UMSC transplantation was successful in recovering some corneal transparency in injured corneas of wild-type, Col5a1 f/f and Col5a1 ∆st/∆st mice. (molvis.org)

In total, we identified 17 neurological indications in clinical stage development. (sscb-stembiotech.com)

2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
Brain organoids mimic more closely the in vivo tissue composition and architecture and are more physiologically relevant than monolayer cultures. (ehu.eus)
Autoimmune side effects, however, remain a risk, emphasizing the relevance of a suicide mechanism allowing in vivo elimination of infused T cells. (haematologica.org)
UMSC transplantation with a fibrin plug improves the healing of injured corneas and regeneration of transparent corneas, as determined with in vivo HRT II confocal microscopy. (molvis.org)

Foetal brain cell graft is a surgical procedure that can be used as a regenerative treatment for various neurological conditions, but was mainly explored and used specifically for treating Parkinson's disease (PD). (wikipedia.org)
Since their discovery, stem cells have ushered in a new era in regenerative medicine. (ehu.eus)
It has been found that some of the SVF cell types possess regenerative and anti-inflammatory potentials in damaged tissues due to their ability to secrete growth factors and anti-inflammatory molecules. (jcadonline.com)
Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)

Stem cells may be derived from adult tissues but the most potent are extracted from developing human embryos. (edu.au)

Stem cell technologies have opened up new avenues in the study of human biology and disease. (ehu.eus)
They therefore represent a more realistic cellular environment for modeling the cell biology and pathology of the nervous system. (ehu.eus)

Associated with new knowledge on the human genome, it could be used to facilitate genotype selection and encourage social and parental intolerance of disability or, potentially, perceived genetic defects. (who.int)
To clearly illustrate the importance of cell and gene therapy in hereditary anemia, this paper provides a review of the genetic aberration, epidemiology, clinical features, current management, and cell and gene therapy endeavors related to SCD, thalassemia, Fanconi anemia, and G6PDD. (sscb-stembiotech.com)
The pace of scientific development has been directly promoted by substantial increases in OECD (Organisation for Economic Cooperation and Development) government funding for genetic and biotechnological research. (edu.au)

At last, scientists had devised a way to reprogram a person's own T cells to attack tumor cells. (ajmc.com)
The key to curing every single cancer, which is our goal, is to have receptors that can recognize the tumor but don't recognize the normal cells. (ajmc.com)
The aim of adoptive T-cell therapy of cancer is to selectively confer immunity against tumor cells. (haematologica.org)

These 2 members mediate leukocyte adhesions to endothelial cells but they also serve as receptors for iC3b (inactivated C3b). (medscape.com)
This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)
First, the cells roll along endothelial surfaces, a process that requires expression of P and E selectins on the endothelial cells and their ligands on leukocytes. (medscape.com)
Rolling is a reversible process, partly because E selectin is shed from the cell surface and P selectin is internalized in endothelial cells. (medscape.com)

Foetal tissue transplantation is a foetal allograft procedure, using tissues from an aborted foetus and implanting it into a diseased patient's body to improve defective tissue functioning. (wikipedia.org)

In an attempt to expand the donor pool, several groups are beginning to explore the use of alternative sources of stem cells such as haploidentical donors and umbilical cord cell blood. (johnshopkins.edu)

Contrary to popular belief, stem cells are present in the human body throughout life and are found in many adult organs. (jcpa.org)
The ICM continues to differentiate into three germ layers-ectoderm, mesoderm and endoderm, each of which follows a specific developmental destiny that takes them along an ever-specifying path at which end the daughter cells will make up the different organs of the human body. (thefutureofthings.com)

Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
3. There is another type of diabetes that affects pregnant women, it is called Gestational Diabetes where the hormones released by the placenta to sustain the pregnancy, make the pregnant woman's cells more resistant to insulin. (livestemcells.asia)

if the foetal neurons are not completely functionally integrated in the host, it may cause various side effects, which are the risks and limitations associated with this procedure. (wikipedia.org)
Cells dissected are glial cells that regulate the neurons' growth, and synaptic connections. (wikipedia.org)
During the first two hours after the injury, which is called the acute phase 4 , glial cells and neurons at the site of the lesion die via necrosis (inflammatory cell death) or by apoptosis (programmed cell death) 5 . (anova-irm.com)
Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
CRT aims to replace neurons that have degenerated in PD, with donor cells that have the potential to functionally re-integrate into the host circuitry. (lu.se)
Previously, it was thought that transplanted neurons could not extend axons over long distances rendering transplantation into the SN a non-viable approach. (lu.se)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)

In the early 90's, Washington University Medical Center reported the success of the first transplant of the pancreatic islet tissue from a donor in a person ailing from Type 1 diabetes. (livestemcells.asia)

While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)

This environment raises ethical questions about consent and ownership of tissues, the cost-effectiveness of harvesting and storage of UCB, undue influence on donors, and issues of distributive justice such as the fact that UCB, which is potentially life-saving and could be easily obtained, may become a resource unfairly restricted only to the wealthy. (sajbl.org.za)
This potentially wide assortment of genetically foreign cells in women, including maternal microchimeric cells from their mother and unique fetal microchimeric cells from each pregnancy raises fundamental new questions about how microchimeric cells interact with each other, and the limits of their accumulation. (fox59.com)
The recent desperation to clone human embryos may be seriously undermining accepted ethical principles of medical research, with potentially profound wider consequences. (lifeissues.net)
Minimally invasive MSC injections need C-arm as guidance that potentially influence the cell viability and doubling time. (ui.ac.id)

Eilon Sharon, lead author of the study and postdoctoral research fellow at Stanford, said that the team came up with a computational approach that relies on knowledge of SNP allele frequencies in populations. (genomeweb.com)
The new study in Science reports that maintaining protective memory suppressive T cells is mediated by tiny populations of baby cells that remain in mothers after pregnancy called fetal microchimeric cells. (fox59.com)

Between 1988 and 1994, roughly 140 Parkinson's disease patients received fetal tissue (up to six fetuses per patient), with varying results. (lozierinstitute.org)
Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)

Summary The curative potential of BMT in SCD is irrefutable, with outstanding results in children following a myeloablative conditioning regimen and a matched sibling donor transplant. (johnshopkins.edu)

Iwijn De Vlaminck, an assistant professor of biomedical engineering at Cornell and senior author of the study, said that previous work had identified that donor-derived cell-free DNA is a "good marker of transplant rejection" because increased levels of donor cfDNA indicates more damage to the graft and thus, rejection. (genomeweb.com)
The next step is then to determine what proportion of donor-derived cfDNA indicates transplant rejection. (genomeweb.com)

We initially used this technology for malaria testing of blood donors and for testing maternal blood for fetal DNA to aid in diagnosing hemolytic disease of the newborn. (blood.ca)
This influence linked to fetal cells builds on research Way and colleagues published in Cell in 2015 that shows children maintain a small supply of cells transferred from their mothers during pregnancy called maternal microchimeric cells. (fox59.com)
Meanwhile, once a grown daughter becomes pregnant, fetal microchimeric cells displace maternal microchimeric cells causing her to immunologically "forget" her mother. (fox59.com)

Also, foetal cells differentiate in response to their surrounding environment, and their location plays a key role, for instance, the functional connections they establish, their growth, elongation and migration are facilitated by the location and environmental cues. (wikipedia.org)

A standardised procedure is followed: the cells are usually obtained from a 7-8 weeks old foetus and the collected cells undergo testing to examine whether they are free from infectious agents and safe for transplantation. (wikipedia.org)
Grafting is a surgical procedure involving the replacement of damaged or missing body tissues from a healthy body, in which blood supply of the surgical area integrates with the neighbouring cells in the body. (wikipedia.org)
In brief, the procedure is to first obtain cells from a healthy donor, and then anaesthetise the patient and make an incision on the target area, for easier access. (wikipedia.org)
Mouse ES cell-derived EBs were dissociated using collagenase, followed with a modified procedure by Isenberg and Klockner in 1982 to prepare the calcium-tolerant ventricular myocytes [ 18 ]. (biomedcentral.com)
2009). After 10 days of PH, one-third of rat/mouse liver tissue that remained after the PH procedure could regenerate and fill the empty liver mass. (ebrary.net)

Before the announcement in February 1997 of the cloning of a sheep by somatic cell nuclear transfer, existing legislation in a number of countries already precluded human cloning for reproductive purposes, sometimes implicitly. (who.int)
Nuclear transplantation, as it was first called, was later referred to as somatic nuclear transfer or cloning. (asu.edu)

Note that fetal tissue has been taken in a number of cases from fetuses at developmental ages where fetal surgery is now used to correct problems and save lives, and at stages where science now demonstrates that the unborn fetus can feel pain. (lozierinstitute.org)

Col5a1 ∆st/∆st mice display a cloudy cornea phenotype that is ameliorated following intrastromal transplantation of UMSCs. (molvis.org)

Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
Flow cytometry is a laser-based technique used to detect and analyze the chemical and physical characteristics of cells or particles. (healthcare-in-europe.com)

Direct neuronal conversion of resident glial cells is advantageous since they are ubiquitously distributed brain cells able to self-renew and replenish their number, making them ideal candidates for endogenous repair. (lu.se)

Anatomy21

Organisms7

Diseases10

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment43

Phenomena and Processes20

Disciplines and Occupations3

Humanities6

Information Science3

Named Groups4

Health Care9

MSCs50

Umbilical cord3

Cartilage16

Therapeutic18

Stromal5

Differentiation12

Diseases13

Transplants14

Thymus2

Hematopoietic Stem Cell Trans2

Bovine2

Trials15

Embryonic stem cel10

Stem cell res11

Sickle-cell di3

Vitro11

Embryo9

Progenitor5

Involves6

Methods7

Allogeneic6

Pluripotent11

Recipient12

Organ15

Liver transplantation2

Serum1

Therapies6

Antigen8

Marrow Transplantation2

Autologous bone marrow2

Adipose tissue derived mesenchymal2

Severe5

Corneal5

Indications1

Vivo4

Regenerative4

Adult tissues1

Biology2

Genetic3

Tumor3

Endothelial cells4

Allograft1

Haploidentical1

Organs2

Placenta2

Neurons8

Pancreatic islet1

Cord blood2

Potentially4

Populations2

Parkinson's2

Curative1

Transplant rejection2

Maternal3

Differentiate in response1

Procedure5

Somatic2

Developmental1

Phenotype1

Characteristics2

Glial cells1