Reprogramming adult cells. Medical search. Frequent questions

In recent years, induced pluripotent stem (iPS) cells have generated a great deal of interest as a potentially unlimited source of various cell types for transplantation. (eurekalert.org)
This is the first proof-of-principle of therapeutic application in mice of directly reprogrammed "induced pluripotent stem" (IPS) cells, which recently have been derived in mice as well as humans. (medicalxpress.com)
Induced pluripotent stem (iPS) cells that result from the reprogramming of somatic cells to a pluripotent state by forced expression of defined factors are offering new opportunities for regenerative medicine. (nyu.edu)
Because the reprogramming of adult human terminally differentiated somatic cells into induced pluripotent stem cells (hiPSCs) became possible in 2007, only eight years have passed. (biopaqc.com)
However, during the generation process of induced pluripotent stem (iPS) cells, genetic manipulation of certain factors may cause tumorigenicity, which limits further application. (ewha.ac.kr)
These old cells were reprogrammed in vitro to induced pluripotent stem cells (iPSC) and to rejuvenated and human embryonic stem cells (hESC): cells of all types can again be differentiated after this genuine "rejuvenation" therapy. (eurekalert.org)
In 2015, Dr. Qiang returned to Drexel University, where he now leads a research laboratory that focuses on using human induced pluripotent stem cell (hiPSC) based technologies to develop in vitro and ex vivo cellular and tissue models. (drexel.edu)
The research team first sought to improve previously established methods for reprogramming of adult cells into so-called induced pluripotent stem (iPS) cells, which look and behave similarly to embryonic stem cells and can differentiate into many different cell types. (hopkinsmedicine.org)
The clinical trials come after researchers at Japan's Kyoto University successfully used human induced pluripotent stem cells (iPS) to restore functioning brain cells in monkeys last year. (deccanchronicle.com)
As an alternative approach, MSCs derived from induced pluripotent stem cells (iPSCs), which may lead to further standardized-cell preparations. (biomedcentral.com)
The REAC action bypassed a persistent reprogramming toward an induced pluripotent stem cell-like state and involved the transcriptional induction of the NADPH oxidase subunit Nox4. (nih.gov)
The resulting cells are nonproliferating and present an alternative to induced pluripotent stem cells for obtaining patient- and disease-specific neurons to be used for disease modeling and for development of cell therapy. (lu.se)
Using this type of direct conversion approach rather than making them from induced pluripotent stem cells, where they revert into a naïve state, helps to retain the epigenetic age of the patient in the generated interneurons. (lu.se)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
Chad and I have both been principal investigators on the induced pluripotent stem cell (IPSC) grant. (medscape.com)

Applying MPTR to dermal fibroblasts from middle-aged donors, we found that cells temporarily lose and then reacquire their fibroblast identity, possibly as a result of epigenetic memory at enhancers and/or persistent expression of some fibroblast genes. (elifesciences.org)
Lately, his group employed lineage-specific transcription factors for reprogramming cellular identity and generating therapeutic relevant neuronal subtypes from conversion of skin fibroblasts. (michaeljfox.org)
We report that that a single transfer of embryonic stem (ES) cell-derived proteins into primarily cultured adult mouse fibroblasts, rather than repeated transfer or prolonged exposure to materials, can achieve full reprogramming up to the pluripotent state without the forced expression of ectopic transgenes. (ewha.ac.kr)
Researchers first multiplied skin cells (fibroblasts) from a 74 year-old donor to obtain the senescence characterized by the end of cellular proliferation. (eurekalert.org)
To do this, they set out to find a way to transform fibroblasts, a type of cell that contributes to the formation of connective tissue, into heart muscle cells via a process called cellular reprogramming. (news-medical.net)
However, they found adult fibroblasts are not very good at following the instructions and are resistant to reprogramming. (news-medical.net)
We found that if you take cardiac fibroblasts from juveniles, they reprogram very nicely. (news-medical.net)
Hodgkinson and his team discovered that a protein oxygen sensor, Epas1, prevents adult fibroblasts from reprogramming themselves. (news-medical.net)
The researchers were able to harness the regenerative capacity of young cells by blocking Epas1 in adult fibroblasts. (news-medical.net)
When the researchers used the RNA-filled exosomes to instruct the fibroblasts to reprogram themselves in a mouse that had just experienced a heart attack, the results were, according to Hodgkinson, "impressive. (news-medical.net)
2023) Neonatal and adult cardiac fibroblasts exhibit inherent differences in cardiac regenerative capacity. (news-medical.net)
Lineage Reprogramming of Fibroblasts into Proliferative Induced Cardiac Progenitor Cells by Defined Factors. (mtsu.edu)
For example, the methods used to generate nerve cells from patient fibroblasts tend to yield low numbers of cells, and those that are produced are not fully functional. (genengnews.com)
The first part of the thesis (Paper I and II) explores reprogramming of adult human fibroblasts and human glial progenitor cells into GABAergic interneurons in 2D cultures. (lu.se)
In 2018, our research group identified the three transcription factors that allow us to convert fibroblasts, for example from the skin, into type 1 conventional dendritic cells (cDC1). (lu.se)
By viral expression of neural fate determinants, it is possible to directly reprogram mouse and human fibroblasts into functional neurons, also known as induced neurons. (lu.se)
In this study, we show that transplanted human fibroblasts and human astrocytes, which are engineered to express inducible forms of neural reprogramming genes, convert into neurons when reprogramming genes are activated after transplantation. (lu.se)
One way we did this was by converting adult fibroblasts, or skin cells, into interneurons. (lu.se)

Div-Seq: single-nucleus RNA-seq reveals dynamics of rare adult newborn neurons. (nature.com)
He, then, has translated some of this knowledge in the stem cell research field contributing to develop protocols and methods for generating sub-type specific telencephalic neurons through in vitro differentiation of embryonic and neural stem cells. (michaeljfox.org)
Now, for the first time, Dr. Su-Chun Zhang and coworkers at the University of Wisconsin-Madison have successfully generated neurons from reprogrammed nonhuman primate cells, transplanted them back into the same animal's brain, and seen them successfully and cleanly integrate into the local tissue. (sens.org)
To date, these cells-induced neurons-have been poor substitutes for cortical neurons, which could model stroke, or motor neurons, which could model motor neuron disease. (genengnews.com)
One focus of my group lies on a better understanding of the physiological process ofneurogenesis in the adult mouse brain with emphasis on the lineage progression from adult neural stem cells to their neuronal and glial progeny as well as the functional integration of adult-generated neurons into the pre-existing circuit. (unimedizin-mainz.de)
Astrocytes in the adult mouse brain can be reprogrammed into neuronal precursors, then neurons, in vivo. (the-scientist.com)
REBIRTH: Astrocytes (green) in the mouse brain can be reprogrammed into neuronal precursors (red), which can then develop into neurons (nuclei in blue). (the-scientist.com)
Zhang's group found that injection of a single transcription factor-SOX2-is necessary and sufficient to convert nonneuronal cells to neuronal precursors called neuroblasts, which can then become functional neurons. (the-scientist.com)
In recent years reprogramming techniques, differentiation protocols, and sequencing analysis have opened a field for generating and studying human-derived neurons with greater potential than ever before. (lu.se)
Following the establishment of successful conversion protocols, we then introduce a 3D model for direct conversion that replicates the cell-cell interactions pivotal for physiological relevance, leading to an accelerated induction of functional neurons (Paper III). (lu.se)
Together these papers encompass a collection of ways to derive human subtype- specific neurons in vitro and serve as platforms for both reprogramming and differentiation studies. (lu.se)
Using a transgenic mouse model to specifically direct expression of reprogramming genes to parenchymal astrocytes residing in the striatum, we also show that endogenous mouse astrocytes can be directly converted into neural nuclei (NeuN)-expressing neurons in situ. (lu.se)
Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)
The discovery she made as a young researcher started the new field of direct reprogramming to replace degenerated neurons. (lu.se)

Recent work has demonstrated that the epigenome is already rejuvenated by the maturation phase of somatic cell reprogramming, which suggests full reprogramming is not required to reverse ageing of somatic cells. (elifesciences.org)
This approach could become an alternative to somatic cell nuclear transfer (SCNT), a method that is currently used to produce human stem cells. (news-medical.net)
Furthermore, Eggan noted that genetic analyses of the fused cells revealed that the somatic cell genes characteristic of adult cells had all been switched off, while those characteristic of embryonic cells had been switched on. (news-medical.net)
The long term goal for this experiment was to do cell fusion in a way that would allow the elimination of the embryonic stem cell nucleus to create an embryonic stem cell from the somatic cell," said Melton. (news-medical.net)
Twenty years have passed since Dolly the sheep was born by cloning (somatic cell nuclear transfer, SCNT) but the results of non-human mammalian cloning are very poor, and cause animal diseases and huge biological losses. (sibi.org)
True cloning performed by nuclear transfer from an adult and differentiated somatic cell to a previously enucleated egg (somatic cell nuclear transfer, SCNT), gives rise to a new cell, the nuclovulo (nucleus+ovum), distinct from the zygote because the sperm is not involved in its creation, while both can develop as embryos and give rise to offspring. (sibi.org)
Prior to SCNT, the somatic cell (differentiated) must be reprogramed to a similar state of a pluripotent embryonic cell (undifferentiated) before the nucleus is extracted and transferred. (sibi.org)
Despite immense promise, somatic cell reprogramming still faces a critical challenge. (sibi.org)
Cellular reprogramming is a new and rapidly emerging field in which somatic cells can be turned into pluripotent stem cells or other somatic cell types simply by the expression of specific combinations of genes. (lu.se)
article{4b5db5d0-c1b6-463c-9ca2-f61b01429744, abstract = {{Cellular reprogramming is a new and rapidly emerging field in which somatic cells can be turned into pluripotent stem cells or other somatic cell types simply by the expression of specific combinations of genes. (lu.se)
This cell then has therapeutic cloning: the global the capacity to divide and grow into an exact replica of the original from whom the debate somatic cell was taken. (who.int)

Our approach starts with purifying the cell type we want and then manipulating it to give those cell types characteristics of progenitor cells, which can grow rapidly but produce only a few cell types. (eurekalert.org)
Somatic cells can be directly reprogrammed to alternative differentiated fates without first becoming stem/progenitor cells. (nih.gov)
Scientists have coaxed differentiated cells to become neuronal progenitor cells many times in vitro. (the-scientist.com)
Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)
Progenitor cells can be far easier to handle in culture than ES cells. (ddw-online.com)
For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)

In vitro assays have also been performed to assess whether the lentiviral vector could potentially transform cells. (ca.gov)
It can be concluded that reprogramming somatic cells in vivo may offer a potential approach to induce enhanced pluripotency rapidly, efficiently, and safely compared to in vitro performed protocols and can be applied to different tissue types in the future. (nyu.edu)
Protein-iPS cells were biologically and functionally very similar to ES cells and differentiated into 3 germ layers in vitro. (ewha.ac.kr)
They then completed the in vitro reprogramming of the cells. (eurekalert.org)
There, he played a vital role in establishing an in vitro platform of the blood-brain barrier for drug screenings in the central nervous system, as well as a CNS-derived microvasculature environment to support neuronal reprogramming. (drexel.edu)
The main result reported by the authors was as follows: "The use of phosphomutant Ascl1 in place of the wild-type protein significantly promotes neuronal maturity after human fibroblast reprogramming in vitro. (genengnews.com)
In order to promote the adult phenotype, stress-inducing agents have been employed in vitro in an attempt to mimic aging. (sloankettering.edu)
Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)

To create specialized cell types for use in cell therapy requires only that we insert the genes (or use non-transgenic approaches) and then test the drug dose and timing required for each cell type and each patient, so it should be relatively scalable at low cost compared to other approaches using each patient's own cells," Waddell says. (eurekalert.org)
These cells were modified by a standard lab technique employing retroviruses customized to insert genes into the cell's DNA. (medicalxpress.com)
The inserted genes were Oct4, Sox2, Lif4 and c-Myc, known to act together as master regulators to keep cells in an embryonic-stem-cell-like state. (medicalxpress.com)
Potential alternatives include other forms of viruses, synthesized versions of the proteins created by the four master regulator genes that are modified to enter the cell nucleus, and small molecules, Hanna says. (medicalxpress.com)
His demonstration that the expression of four master regulatory genes was sufficient to cause the reprogramming of adult cells has opened up many possibilities for human stem cell therapies. (brandeis.edu)
Overall, we demonstrate that it is possible to separate rejuvenation from complete pluripotency reprogramming, which should facilitate the discovery of novel anti-ageing genes and therapies. (elifesciences.org)
After testing several different genes, they were able to improve reprogramming efficiency by adding a viral protein known as SV40 large T antigen. (hopkinsmedicine.org)
Using both fetal and adult human skin cells, the researchers introduced the four genes previously reported sufficient for cell reprogramming and compared the efficiency of reprogramming in the presence or absence of large T antigen. (hopkinsmedicine.org)
The scientists found that radiation treatment triggered heart muscle cells to begin expressing different genes. (wustl.edu)
With the exception of a few genes one way or the other -- which is perhaps because these cells are now tetraploid -- the hybrid cells are indistinguishable from human embryonic stem cells," he said. (news-medical.net)
But previous approaches required the use of viruses to deliver the four genes needed to activate the cell and accomplish that task. (cbc.ca)
The model also predicts that reprogramming the network from a differentiated state, in particular the endoderm state, into a stem cell state, is best achieved by over-expressing Nanog, rather than by suppression of differentiation genes such as Gata-6. (lu.se)
By triggering certain genes, researchers may be able to cause the stem cells to specialize and become the cells that need to be replaced. (msdmanuals.com)
One way to induce these cells is to inject them with material that affects their genes, a process called reprogramming. (msdmanuals.com)
We have the same genes everywhere in the cells of the body, yet certain types of protein are expressed in the brain, while others are expressed in the liver. (lu.se)

Direct neuronal conversion of resident glial cells is advantageous since they are ubiquitously distributed brain cells able to self-renew and replenish their number, making them ideal candidates for endogenous repair. (lu.se)

A major block in the critical path of regenerative medicine is the lack of suitable cells to restore function or repair damage," says co-senior author Tom Waddell, a thoracic surgeon at the University of Toronto. (eurekalert.org)
According to the authors, the approach could be used for a variety of regenerative medicine practices, including cell replacement therapy, disease modelling, and drug screening for human diseases. (eurekalert.org)
While IPS cells offer tremendous promise for regenerative medicine, scientists caution that major challenges must be overcome before medical applications can be considered. (medicalxpress.com)
Transcription factor over-expression is a proven method for reprogramming cells to a desired cell type for regenerative medicine and therapeutic discovery. (nature.com)
Dr. Irving L. Weissman is professor of pathology and developmental biology at the Stanford University School of Medicine , where he is director of the Institute of Stem Cell Biology and Regenerative Medicine. (brandeis.edu)
We conclude by talking about open questions associated with these concepts and exactly how their quality might provide to fortify the guarantee of pluripotent stem cells in regenerative medication. (biopaqc.com)
The concept of reprogramming of somatic cells has opened a new era in regenerative medicine. (ewha.ac.kr)
The results represent significant progress for research into iPSC cells and a further step forwards for regenerative medicine. (eurekalert.org)
Setback for regenerative medicine Stem cells derived from reprogramming adult cells may have limited usefulness as an alternative to embryonic stem cells, leading researchers said. (bioedge.org)
Our results show for the first time the feasibility of using a physical stimulus to afford the expression of pluripotentiality in human adult somatic cells up to the attainment of three major target lineages for regenerative medicine. (nih.gov)
The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)
Such an approach is highly relevant to regenerative medicine since it allows for a rapid search over the host of possibilities for reprogramming to a stem cell state. (lu.se)

The scientists studied a therapeutic application of IPS cells with the sickle-cell anemia model mouse developed by the laboratory of Tim Townes of the University of Alabama at Birmingham. (medicalxpress.com)
For the foreseeable future, there will remain a continued need for embryonic stem cells as the crucial assessment tool for measuring the therapeutic potential of IPS cells. (medicalxpress.com)
He was the first to appreciate the therapeutic potential of stem cell therapy and has pioneered its development. (brandeis.edu)
This research paves the way for the therapeutic use of iPS, insofar as an ideal source of adult cells is provided, which are tolerated by the immune system and can repair organs or tissues in elderly patients. (eurekalert.org)
His lab is also committed to preclinically testing the therapeutic efficacy of cellular reprogramming-based strategies for treating CNS injuries and diseases. (drexel.edu)
A particularly attractive therapeutic strategy is to convert liver adult cells of diabetic patients into functional pancreatic β cells through lineage reprogramming. (europa.eu)
As well as making more mature nerves, Dr. Philpott's lab is now using similar methods to improve the function of insulin-producing pancreas cells for future therapeutic applications. (genengnews.com)
Cellular therapy based on mesenchymal stem cells (MSCs) is a promising novel therapeutic strategy for the osteonecrosis of the femoral head (ONFH), which is gradually becoming popular, particularly for early-stage ONFH. (biomedcentral.com)
With the hope that stem cells can replace diseased or damaged cells, tissues, and organs, the therapeutic potential of stem cells is enormous. (cellmedicine.com)
The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)

The study also suggests that the same cellular reprogramming effect could be achieved with lower doses of radiation, opening the door to the possibility of wider uses for radiation therapy in different types of cardiac arrhythmias. (wustl.edu)
Cellular reprogramming, coupled with reversing cellular aging, has limitless future applications, including restoring neuron loss in the brains of dementia patients and eliminating skin scarring in psoriasis patients without invasive surgical interventions, Hodgkinson said. (news-medical.net)
That process has been called 'cellular reprogramming' and was recognized as the breakthrough of the year for 2008 by Science. (cbc.ca)
One option to get hematopoietic stem cells matching perfectly a patient is cellular reprogramming. (biorxiv.org)
Using a combination of gene expression profiling and gene regulatory network analysis, we found that endothelial cells from the pancreas, brain, kidney and liver appear to be the most suitable targets for cellular reprogramming into hematopoietic stem cells. (biorxiv.org)
We focused particularly on harnessing reprogramming, or direct cellular reprogramming, to induce antigen presentation in cancer cells. (lu.se)

Methylation reprogramming was correlated with increased gene expression variability. (jneurosci.org)
DNA methylation reprogramming in the DRG thus contributes to nerve injury-induced chronic pain. (jneurosci.org)

So, we tried to understand whether the aging process was actually interfering with fibroblast reprogramming. (news-medical.net)
In the studies published in Science, the researchers combined human fibroblast cells with human embryonic stem cells in the presence of a detergent-like substance that caused the two cell types to fuse. (news-medical.net)
The researchers demonstrated that they had achieved fusion of the two cell types by searching the fused cells for two distinctive genetic markers present in the somatic fibroblast and stem cells. (news-medical.net)
F urther complicating matters, human ES cells are typically co-cultured with feeder layers of mouse fibroblast cells. (ddw-online.com)
Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)
Several growth factors have been identified that promote growth of human ES cells in culture, most notably basic fibroblast growth factor (bFGF). (ddw-online.com)

We hypothesized that the reprogramming of somatic cells towards pluripotency could be achieved in vivo by gene transfer of reprogramming factors. (nyu.edu)
Oct3/4 acts to keep pluripotency in the internal cell mass (ICM) from the blastocyst. (biopaqc.com)
Background: Recent studies have associated the transcription factors, Oct4, Sox2 and Nanog as parts of a self-regulating network which is responsible for maintaining embryonic stem cell properties: self renewal and pluripotency. (lu.se)

In vivo, NK cells had blunted antitumor responses and failed to reduce tumor growth in obesity. (nih.gov)
In vivo evaluation of TCR-replaced Tscm cells for anti-glioma effects in comparison with Tscm engineered with the conventional retroviral TCR vector and CRISPR-knock out of eTCR. (ca.gov)
In order to efficiently reprogram cells in vivo, high levels of the Yamanaka (OKSM) transcription factors need to be expressed at the target tissue. (nyu.edu)
This provided proof-of-evidence of in vivo reprogramming of adult, somatic cells towards a pluripotent state with high efficiency and fast kinetics. (nyu.edu)
Furthermore, protein-iPS cells possessed in vivo differentiation (well-differentiated teratoma formation) and development (chimeric mice generation and a tetraploid blastocyst complementation) potentials. (ewha.ac.kr)
In vivo reprogramming of astrocytes to neuroblasts in the adult brain," Nat Cell Biol , 15:1164-75, 2013. (the-scientist.com)
But this had not been accomplished in vivo until Chun-Li Zhang of the University of Texas Southwestern Medical Center in Dallas tried injecting different combinations of reprogramming candidates into the brains of older mice. (the-scientist.com)
In addition, because the cells do not pass a stem cell intermediate, direct neural conversion has the potential to be performed in vivo. (lu.se)

Alexandra Gabriela Barros Ferreira defends her Ph.D. thesis "Restoring antitumor immunity with dendritic cell reprogramming strategies" on Friday, 17 November, 2023. (lu.se)
Andreas Bruzelius defended his Ph.D. thesis 'Generating GABAergic interneurons through reprogramming and differentiation strategies' on Friday, 20 October 2023. (lu.se)
Cell Reports, Available online 14 November 2023. (lu.se)

This method involves genetically reprogramming skin cells taken from adult donors to an embryonic stem-cell-like state, growing these immature cells to large numbers, and then converting them into specialized cell types found in different parts of the body. (eurekalert.org)
Mice with a human sickle-cell anemia disease trait have been treated successfully in a process that begins by directly reprogramming their own cells to an embryonic-stem-cell-like state, without the use of eggs. (medicalxpress.com)
Researchers at Johns Hopkins have established a human cell-based system for studying sickle cell anemia by reprogramming somatic cells to an embryonic stem cell like state. (hopkinsmedicine.org)
Without large T, cells form embryonic stem cell-like clusters in three to four weeks. (hopkinsmedicine.org)
Embryonic stem cell-like clusters were visible 14 days after they initiated reprogramming and from these clusters the researchers established three different cell lines that both look and behave like human embryonic stem cells. (hopkinsmedicine.org)
Critics of embryonic stem cell research, which include the Roman Catholic Church, praised the discovery. (cellmedicine.com)

Reprogramming" of adult differentiated cells into pluripotent stem cells is an exciting method in biology that holds enormous promise for rejuvenation biotechnology. (sens.org)

To arrive at such results, suggested Dr. Philpott, "not only do you have to think about how you start the process of cell differentiation in stem cells, but you also have to think about what you need to do to make differentiation complete-we can learn a lot from how cells in developing embryos manage this. (genengnews.com)
Researchers there are working on technology that induces human skin cells to change into the kind of stem cells that have been created by embryos. (cbc.ca)
Some argue that the possibility of mimicking stem cells without acquiring them from embryos, side-steps that moral dilemma. (cbc.ca)
Embryonic stem cells come from embryos, embryonic germ cells from testes, and adult stem cells can come from bone marrow. (cbc.ca)
This new method of generating stem cells does not require embryos as starting points and could be used to generate cells from many adult tissues, such as a patient's own skin cells,' said principal author Andras Nagy, senior investigator at Mount Sinai's Samuel Lunenfeld Research Institute. (cbc.ca)
For the first time, researchers created stem cells without using embryos. (mit.edu)
In November, Yamanaka's team reported the creation of cell that were indistinguishable from those taken from human embryos. (cellmedicine.com)
Since viable embryos had to be destroyed to extract embryonic stem cells (cells which are thought to have the greatest potential among all stem cells), stem cell research was highly controversial: until now. (cellmedicine.com)
Of the 630 transplanted embryos, only seven grew into offspring mice, but the mice that grew up were fertile as adults, Hayashi reports. (gigazine.net)
Stem cells can be obtained from the embryos that are not used. (msdmanuals.com)
Because the embryos then lose the ability to grow into a complete human being, the use of stem cells from embryos is controversial. (msdmanuals.com)
The stem cells suits human needs, does not cause harm and can be obtained from both adult and fetal does not conflict with religious beliefs, it has tissues, umbilical cord and early embryos. (who.int)
Unicellular for those cells that are derived from human organisms are primed to replicate (clone) pre-embryos, which seem to have a high themselves by nature. (who.int)

Next, the researchers followed a well-established protocol for differentiating embryonic stem cells into precursors of bone marrow adult stem cells, which can be transplanted into mice to generate normal blood cells. (medicalxpress.com)
Since there is a delay in the appearance of the T cells that come from stem cells to get out of the bone marrow and into the blood, we will give patients both gene-modified T cells for a first wave of antitumor activity and gene-modified stem cells which will provide a bridge until the stem cells have produced more T cells. (ca.gov)
We have demonstrated that when mouse stem cells are gene-modified with this lentiviral vector, the stem cells take up residence in the bone marrow and produce appropriate blood cells. (ca.gov)
They demonstrated that transferring functional mitochondria of bone marrow-derived stem cells to defective parenchymal cells increases the aerobic respiration capacity of recipient mitochondria ( 7 ). (frontiersin.org)
Blood is preferred to bone marrow as a source because the procedure is less invasive and the number (count) of blood cells returns to normal more quickly. (msdmanuals.com)
The bone marrow and blood of children and adults contain stem cells. (msdmanuals.com)

We further explore the contribution of various glial cells in a 3D co-culture setting with interneurons employing both functional and molecular analyses. (lu.se)
Rather than ignoring the result, she chose to follow up on indications that it was possible that radial glial cells generate nerve cells. (lu.se)
I wanted to find out in which cells a specific transcription factor was expressed, but what I observed led me to believe that it might be possible that the glial cells in the developing brain generate nerve cells", explains Götz. (lu.se)

We will develop a non-viral gene editing technology to replace the endogenous TCRα locus of stem memory T cells with transgene TCRs that are specific to brain cancer neoantigens. (ca.gov)
Taken together, our data provide proof of principle that direct neural conversion can take place in the adult rodent brain when using transplanted human cells or endogenous mouse cells as a starting cell for neural conversion. (lu.se)

However, researchers at the Harvard Stem Cell Institute say reprogrammed cells won't eliminate the need or value of studying embryonic stem cells. (cbc.ca)
We're thrilled to have Chad Cowan, an associate professor at Harvard University who is at the Harvard Stem Cell Institute. (medscape.com)

Since 2007, a handful of research teams across the world have been capable of reprogramming human adult cells into induced pluripotent cells (iPSC), which have similar characteristics and potential to human embryonic stem cells (hESC). (eurekalert.org)

A major advantage of this approach is the ability to generate patient-specific iPS cells for transplantation, thereby minimizing the risk of harmful immune reactions. (eurekalert.org)
Due to serious organ shortage for pancreas transplantation, cell-based therapy represents the most promising option and different potential sources for new β cells are currently being explored, including embryonic stem cells and other cell types. (europa.eu)
In addition, it could eventually generate mature nerve cells for transplantation into patients with a range of neurodegenerative diseases. (genengnews.com)
We also discussed the advantage and challenges of mitochondrial transfer strategies, including cell-based mitochondrial transplantation, extracellular vesicle-based mitochondrial transplantation, and naked mitochondrial transplantation, for the treatment of cardiovascular disorders. (frontiersin.org)
The transplantation of these cells is one of the most common treatments to cure patient suffering of blood diseases. (biorxiv.org)
Stem cell transplantation is the removal of stem cells (undifferentiated cells) from a healthy person and their injection into someone who has a serious blood disorder. (msdmanuals.com)
Overview of Transplantation Transplantation is the removal of living, functioning cells, tissues, or organs from the body and then their transfer back into the same body or into a different body. (msdmanuals.com)
But researchers think that these stem cells have the most potential for producing different kinds of cells and for surviving after transplantation. (msdmanuals.com)
These stem cells are most often used for transplantation. (msdmanuals.com)

The goal of this project was to develop a non-viral gene-editing technology to replace a T-lymphocyte's own gene for T cell receptor with another receptor gene that would allow the T-lymphocyte to recognize a target molecule that is uniquely present on a highly aggressive type of brain tumors. (ca.gov)
One possible reason could be insufficient survival of T cells in the mice and/or numbers of T-lymphocytes that were able to arrive in the brain tumors. (ca.gov)
Our institution is one of the largest brain tumor centers in the world, developing a number of innovative clinical trials and treating patients primarily from CA. The proposed study will establish a strong basis to develop a novel, safe and effective stem memory T cell therapy for patients with malignant brain tumors, including ones in CA. (ca.gov)
Parkinson's is caused by a lack of dopamine made by brain cells and researchers have long hoped to use stem cells to restore normal production of the neurotransmitter chemical. (deccanchronicle.com)
They can then be coaxed into dopamine-producing brain cells. (deccanchronicle.com)
Andreas' research focuses on generating brain cells, specifically interneurons, in the lab, which has significant implications for the study of neurodegenerative and neuropsychiatric disorders. (lu.se)

Among the chromatin accessibility methods, complex methods DeepAccess and diffTF have higher correlation with the ranked significance of transcription factor candidates within reprogramming protocols for differentiation. (nature.com)
We provide evidence that AME and diffTF are optimal methods for transcription factor recovery that will allow for systematic prioritization of transcription factor candidates to aid in the design of new reprogramming protocols. (nature.com)
Fig. 1: Identifying transcription factors that reprogram starting cells to target cell types. (nature.com)
Fig. 3: Use of histone mark and EP300 annotation does not significantly affect transcription factor recovery in liver cells. (nature.com)
By manipulating the signals that transcription factors send to the cells, the researchers, led by Anna Philpott, Ph.D., were able to promote cell differentiation and maturation, even in the presence of conflicting signals that were directing the cell to continue dividing. (genengnews.com)
Specifically, the researchers accomplished the cell-cycle-dependent phosphorylation of a key reprogramming transcription factor, Ascl1, on multiple serine-proline sites. (genengnews.com)
Although transcriptional similarities have been found between embryonic and adult endothelial cells, we found some key differences in term of transcription factors expression. (biorxiv.org)

Despite significant progress, these protocols remain limited by low yield and purity of the desired mature cell types, as well as the potential of immature cells to form tumors. (eurekalert.org)
The magnitude of rejuvenation instigated by MPTR appears substantially greater than that achieved in previous transient reprogramming protocols. (elifesciences.org)
The method performs favorably compared to other transient reprogramming protocols, and the study will be of interest to developmental biologists as well as researchers who study ageing. (elifesciences.org)
Currently, differentiation protocols generate cells with an immature phenotype. (sloankettering.edu)

All you need is a basic technology, cell biology" and "you don't need special technology or equipments. (cellmedicine.com)
In 2020, Professor Katsuhiko Hayashi, who studies genome biology at the Graduate School of Medicine, Osaka University, started a project to create eggs using cells collected from adult male mice. (gigazine.net)
At the core of the network reside Oct4, Sox2 and Nanog, into embryonic stem cells [1,2,3,4,5], have made major inroads which form a self-organized core of the TFs maintaining into stem cell biology. (lu.se)
Combining cell biology and electrophysiology, his work has the potential to create personalized disease models for future research. (lu.se)
One of the greatest controversies triggered tissue, a stem cell encoding for heart tissue by the rapid pace of evolution in biology, will eventually develop into heart tissue particularly in genomics and biotechnology, and so on. (who.int)

In 1962 he made the stunning observation that it was possible to take a differentiated adult cell from a frog and to re-set its genetic program so that the reprogrammed nucleus could be implanted in an enucleated egg and direct the development of tadpoles. (brandeis.edu)
I believe that the reprogramming errors are not the only cause of these low rates of cloning: the mammalian SCNT fails with a very high frequency mainly due to the damage that the technique itself inflicts in the egg and the somatic nucleus, and the very few successful cases occur only when the damage is not significant. (sibi.org)
When the nucleus of a stem cell has been the technique of cloning. (who.int)
The con- is removed and replaced by a nucleus of cept of human cloning has long been in the another cell type, the stem cell will then imagination of many scientists, scholars and be reprogrammed to produce the product fiction writers [ 1 ]. (who.int)

They can divide and form all types of differentiated adult cells in the body (neurones, cardiac cells, skin cells, liver cells, etc. (eurekalert.org)
The researchers behind the work used RNAs to instruct cells in an injured heart to eliminate scar tissue and recreate cardiac muscle, allowing the heart to function like new again. (news-medical.net)

The researchers started to genetically reprogram adult Club cells isolated from mice, transiently expressing the four iPS reprogramming factors, but interrupted the process early, prior to reaching the pluripotent state, to generate progenitor-like cells, which are more committed to a specific lineage and show more controlled proliferation than pluripotent cells. (eurekalert.org)
A strategy in the treatment of cancer by harnessing the immune system, called adoptive cell therapy, is to use an individual's own immune cells (T cells) and genetically modify them to target them to kill the cancer. (ca.gov)

Inserm's AVENIR "Genomic plasticity and aging" team, directed by Jean-Marc Lemaitre, Inserm researcher at the Functional Genomics Institute (Inserm/CNRS/Université de Montpellier 1 and 2), has recently succeeded in rejuvenating cells from elderly donors (aged over 100). (eurekalert.org)
Using this new "cocktail" of six factors, the senescent cells, programmed into functional iPSC cells, re-acquired the characteristics of embryonic pluripotent stem cells. (eurekalert.org)
Signs of aging were erased and the iPSCs obtained can produce functional cells, of any type, with an increased proliferation capacity and longevity," explains Jean-Marc Lemaitre who directs the Inserm AVENIR team. (eurekalert.org)
In pathological conditions, damaged cells transfer dysfunctional mitochondria toward recipient cells to ask for help and take up exogenous functional mitochondria to alleviate injury. (frontiersin.org)

These tumors occur in children and young adults. (ca.gov)
Gliomas are lethal tumors often affecting children and young adults. (ca.gov)
Such clinical applications of iPS cells have been limited so far, mainly due to the poor efficiency of the existing reprogramming methodologies and the risk of the generated iPS cells to form tumors upon implantation. (nyu.edu)
Our overall aim is to understand how TAM immunosuppressive and pro-metastatic functions are regulated and if these TAMs can be used as mechanistic, predictive and prognostic tools for different solid tumors, including breast cancer, adult and pediatric sarcoma, and other solid tumors. (lu.se)

To decrease or eliminate possible cancer in the treated mice, the c-Myc gene was removed by genetic manipulation from the IPS cells. (medicalxpress.com)
The patient's own white blood cells and stem cells from their blood are modified in the laboratory using genetic techniques to express a specific receptor against cancer cells. (ca.gov)
Gene modification of cells involves the transfer of foreign genetic material (DNA) into a cell, in this case the immune system cells and stem cells. (ca.gov)
Initially, he established genetic cell reprogramming for generating iPS cells with the aim to model human diseases like Alzheimer's and Parkinson's disease and atypical Rett syndrome. (michaeljfox.org)
To achieve this, they used an adapted strategy that consisted of reprogramming cells using a specific "cocktail" of six genetic factors, while erasing signs of aging. (eurekalert.org)
The fusion causes the adult cells to undergo genetic reprogramming, which results in cells that have the developmental characteristics of human embryonic stem cells. (news-medical.net)
Stem cells also provide a model system in which researchers can study the causes of genetic disease and the basis of embryonic development. (news-medical.net)
Eggan, Melton and their colleagues decided to pursue their alternative route after other researchers had shown that genetic reprogramming can occur when mouse somatic cells are fused to mouse embryonic stem cells. (news-medical.net)
Utilizing samples from a birth cohort, we investigated antibody and B-cell responses to GII.17 cluster variants in confirmed GII.17 infections in young children as well as demonstrated that the distinct genetic clusters co-circulate. (cdc.gov)

Not only did T speed up reprogramming, we also found that it increases the total number of reprogrammed cells, which is great because often in reprogramming, not all cells go all the way," says Cheng, who explains that rigorous follow-up tests are required to determine if the reprogrammed cells really behave like pluripotent embryonic stem cells. (hopkinsmedicine.org)

We provide evidence that the two cell sources can successfully convert into subtype-specific GABAergic interneurons. (lu.se)
The focus has been on GABAergic interneurons and making them from different starting cells. (lu.se)

The researchers showed that the resulting Club-iPL cells could give rise to not only Club cells, but also to other respiratory tract cells such as mucus-secreting goblet cells and ciliated epithelial cells that produce the CFTR protein, which is mutated in patients with cystic fibrosis. (eurekalert.org)
The purpose of the current study is to give gene-modified T cells in combination with gene-modified stem cells to reprogram the immune system to recognize and kill cancer cells that have the NY-ESO-1 protein with sustained killing activity. (ca.gov)
This process will endow the recipient immune cells and descendants of the stem cells with the ability to eliminate cancer cells that express the cancer specific protein, NY-ESO-1. (ca.gov)
We verified that protein-based reprogramming was neither by the contamination of protein donor ES cell nor by DNA/RNA from donor ES cell. (ewha.ac.kr)
Dr. Philpott and her collaborators are aware that the protein control mechanisms that promote neuron maturation are similar to those involved in the maturation of important cells in other tissues such as pancreatic islets, the cell type that fails to function effectively in type 2 diabetes. (genengnews.com)
Mitochondria not only serve as power plants in cells but also act as crucial regulators in many biological processes, including reactive oxygen species (ROS) signaling, redox balance, calcium homeostasis, protein quality control, and programmed cell death ( 3 , 4 ). (frontiersin.org)
Peter Walter has received widespread acclaim for his discoveries of how the cell ensures that its fundamental building blocks are properly constructed and the role protein malformations play in disease," said UCSF Interim Chancellor Sam Hawgood, MBBS. (ucsf.edu)
As Walter put it, "The unfolded protein response makes life and death decisions for the cell. (ucsf.edu)

SCNT involves transferring the nuclei of adult cells, called somatic cells, into oocytes in which scientists have removed the nuclei. (news-medical.net)
Her research involves direct reprogramming approaches, which function like a Trojan horse, enabling cancer cells to be targeted by the immune system. (lu.se)

Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)

Reprogramming can reverse the abnormal proliferation, differentiation, and DNA methylation patterns of ONFH-BMSCs. (biomedcentral.com)

But recent evidence suggests it may be possible to reprogram adult stems to repair tissues. (cbc.ca)
Researchers hope to use stem cells to repair or replace cells or tissues damaged or destroyed by such disorders as Parkinson disease, diabetes, and spinal injuries. (msdmanuals.com)

The researchers have successfully rejuvenated cells from elderly donors, some over 100 years old, thus demonstrating the reversibility of the cellular aging process. (eurekalert.org)
reported that using ES cells successfully produced puppies from two females or from two males. (gigazine.net)
Identifying the right cocktail of media conditions, supplements and growth factors that successfully drive stem cells toward a desired lineage on a reproducible basis is a time-consuming, iterative exercise. (ddw-online.com)

As demonstrated November 30 in the journal Stem Cell Reports , researchers in Canada converted adult mouse respiratory tract cells called Club cells into large, pure populations of induced progenitor-like (iPL) cells, which retained a residual memory of their parental cell lineage and therefore specifically generated mature Club cells. (eurekalert.org)
For their own part, the researchers plan to test this approach with other cell types, including human cells. (eurekalert.org)
The researchers proved that the iPSC cells thus obtained then had the capacity to reform all types of human cells. (eurekalert.org)
To check the "rejuvenated" characteristics of these cells, the researchers tested the reverse process. (eurekalert.org)
One challenge to studying blood diseases like sickle cell anemia is that blood stem cells can't be kept alive for very long in the lab, so researchers need to keep returning to patients for more cells to study," says Cheng. (hopkinsmedicine.org)
In the human heart samples, the researchers found that these changes in heart muscle cells were only present in areas of the heart that received the targeted radiation dose. (wustl.edu)
The researchers formulated a cocktail of RNAs and packaged them into exosomes, a natural product produced by most cells. (news-medical.net)
Researchers have developed a new technique for creating human embryonic stem cells by fusing adult somatic cells with embryonic stem cells. (news-medical.net)
In theory, researchers can induce embryonic stem cells to mature into a variety of specialized cells. (news-medical.net)
For that reason, many researchers believe stem cells offer promise for creating populations of specialized cells that can be used to rejuvenate organs, such as the pancreas or heart, that are damaged by disease or trauma. (news-medical.net)
The researchers were also able to further confirmed that fusion occurred by studying the chromosomal makeup of the fused cells. (news-medical.net)
The researchers also performed fusion experiments using pelvic bone cells as the somatic cells and a different human embryonic cell line, to demonstrate that their technique was not restricted to one adult cell type or embryonic cell line. (news-medical.net)
Researchers have long hoped to use stem cells to restore normal production of the neurotransmitter chemical. (deccanchronicle.com)
Given the complexity of the biological function of mitochondria, researchers have begun to consider rescuing the injured cells through mitochondrial transfer, that is, replacing damaged mitochondria with healthy mitochondria from donor cells. (frontiersin.org)
A separate team of U.S. researchers at the University of Wisconsin at Madison, led by James Thompson, also reprogrammed skin cells at the same time as the Japanese team. (cellmedicine.com)
The UPR that Walter discovered in yeast is also present in humans, which has led other researchers to build on Walter's work and identify two additional, complementary UPR signaling pathways at work in human cells. (ucsf.edu)

My ERC-funded project (ERC Starting Grant "HEPATOPANCREATIC") is focused on the molecular mechanisms underlying cellular plasticity between liver and pancreatic cells. (europa.eu)
Little is known yet about the reprogramming mechanisms. (sibi.org)
By targeting these mechanisms, the overarching aim is to reprogram pro-tumoral TAMs into an anti-tumoral state, affecting various components of the non-cancer cells residing in the tumor microenvironment, ultimately inhibiting tumor growth and metastasis ( Figure 1 ). (lu.se)

To create the IPS cells, the scientists started with cells from the skin of the diseased mice, explains lead author Jacob Hanna, a postdoctoral researcher in the Jaenisch lab. (medicalxpress.com)
The scientists created such precursor cells from the IPS cells, replaced the defective blood-production gene in the precursor cells with a normal gene, and injected the resulting cells back into the diseased mice. (medicalxpress.com)
The scientists knew that if their studies were successful, it would provide the research community with a new option for producing reprogrammed cells using embryonic stem cells, which are more plentiful and easier to obtain than unfertilized human eggs. (news-medical.net)
Japanese scientists said on Monday they will start clinical trials next month on a treatment for Parkinson's disease , transplanting "reprogrammed" stem cells into brains, seeking a breakthrough in treating the neurodegenerative disorder. (deccanchronicle.com)
Scientists have been all abuzz in the last few years over stem cells - cellular magicians that promise to dazzle and amaze. (cbc.ca)
Scientists say embryonic stem cells are the most useful type because they have the potential to become any type of cell within the body. (cbc.ca)
Scientists are fascinated by the ability of stem cells to become any type of cell. (cbc.ca)
Scientists are developing ways of enabling (inducing) other cells (such as a blood or skin cell) to act as stem cells. (msdmanuals.com)

Despite the technological advances in SCNT during the last decade, and its scientiﬁc and medical importance, the molecular processes involved in nuclear reprogramming remain largely unknown and the overall efﬁciency of SCNT in mammals remains very low. (sibi.org)
Additionally, the efﬁciency of nuclear transfer technology may be enhanced by better understanding the nature of reprogramming using the cow model, since approximately half of all SCNT's worldwide are performed in this species [20]. (sibi.org)
Human cells with nuclear phase = 2n have 23 pairs of 46 chromosomes. (gigazine.net)

We were surprised to the extent that it can be fine-tuned by the timing and dosing of the drug used to activate the reprogramming factors. (eurekalert.org)
However, a general method for the identification of reprogramming factors to create an arbitrary cell type is an open problem. (nature.com)
Here we examine the success rate of methods and data for differentiation by testing the ability of nine computational methods (CellNet, GarNet, EBseq, AME, DREME, HOMER, KMAC, diffTF and DeepAccess) to discover and rank candidate factors for eight target cell types with known reprogramming solutions. (nature.com)
We find the best factor identification methods can identify an average of 50-60% of reprogramming factors within the top ten candidates, and methods that use chromatin accessibility perform the best. (nature.com)
Here we have developed the first "maturation phase transient reprogramming" (MPTR) method, where reprogramming factors are selectively expressed until this rejuvenation point then withdrawn. (elifesciences.org)
Although interspecies distinctions in cell-type particular factors exist, eventually and pursuing implantation and gastrulation, 3 specific germ levels emerge: endoderm (which forms the liner of organs), mesoderm (gives rise to bone tissue, muscle tissue, and vasculature), and ectoderm (that results the anxious system and epidermis). (biopaqc.com)
Direct reprogramming strategies with cDC1-specific factors can induce antigen-presentation in cancer cells, making cancer cells visible to the immune system and easier to fight. (lu.se)

1. The Developmental Roots of the Anxious System: A SYNOPSIS The procedure of neurodevelopment can be spatiotemporally controlled and necessitates sequential, intensifying limitations in cell destiny. (biopaqc.com)
As part of the body's response to that injury, cells in the injured portion of the heart appear to turn on some of these early developmental programs to repair themselves. (wustl.edu)
The DNA methylation changes in the injured DRGs recapitulated developmental reprogramming at the neonatal stage. (jneurosci.org)
Michinori Saito, a collaborator and a developmental biologist at Kyoto University, said that using this research method to create eggs from human cells could take a considerable amount of time, and the culture period would be long. (gigazine.net)
Both direct reprogramming and differentiation have benefits and drawbacks, while direct reprogramming can be used to study patient-specific late-onset diseases, differentiation provides developmental information and mechanics. (lu.se)

Inhibiting PPARα/δ or blocking the transport of lipids into mitochondria reversed NK cell metabolic paralysis and restored cytotoxicity. (nih.gov)
Our results demonstrate that the lipotoxic obese environment impairs immunosurveillance and suggest that metabolic reprogramming of NK cells may improve cancer outcomes in obesity. (nih.gov)
supports the concept that metabolic reprogramming, inflammation, and genome instability (including epige- netic changes) underlie many of the other hal marks and foster multiple hallmark functions. (who.int)
In the case of cancer-associated metabolic reprogramming, cancer cel s preferentially metabolize glu- cose through glycolysis rather than oxidative phosphorylation, even in the presence of oxygen [11-13]. (who.int)

Both of these latter techniques risk changes to the metabolism during cell preparation and barcoding prior to cell lysis- since both approaches, generally, rely on the use of live cells 30 . (nature.com)
Commercial approaches have costs ranging from $1 to $5 per cell per expressome. (nature.com)

Immunotherapy can fail in patients with cancer when certain cancer cells can hide from the immune system. (lu.se)
However, cDC1 can be compromised in cancer patients and some cancer cells lack these antigen-presenting proteins on their surface, impacting immunotherapy response. (lu.se)

skin cells of the mouse into cells strikingly similar to embryonic stem cells, capable of development into a mouse. (brandeis.edu)

Although skin cells may be reprogrammed to form other cell types such as nerve cells, the "adult" status of the cell you start with may be lost along the way, perhaps irretrievably. (genengnews.com)
A new method of generating mature nerve cells from skin cells, however, has been developed. (genengnews.com)
The ability to transform skin cells into stem cells isn't new. (cbc.ca)
Adult skin cells were reprogrammed into. (mit.edu)
They accomplished this feat by reprogramming human skin cells. (cellmedicine.com)
Several laboratories in Japan as well as up to five in the United States have also converted human skin cells into pluripotent stem cells, or iPS cells. (cellmedicine.com)
Stem cells are undifferentiated cells that have the potential to become one of 200 types of cells in the body, including blood, nerve, muscle, heart, glandular, and skin cells. (msdmanuals.com)

BMSCs of ONFH patients (ONFH-BMSCs) were reprogrammed to iPSCs, following which the iPSCs were differentiated into MSCs (iPSC-MSCs). (biomedcentral.com)
The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)

In this study, we hypothesize that gene-modified stem cells will allow a sustained production of active T cells with antitumor activity. (ca.gov)

Studies have shown that cells can eliminate defective mitochondria by delivering them to recipient cells (such as macrophages) to maintain homeostasis. (frontiersin.org)
Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)

Anatomy31

Organisms11

Diseases4

Chemicals and Drugs38

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes52

Disciplines and Occupations3

Technology, Industry, Agriculture1

Humanities2

Information Science1

Named Groups1

Health Care1

Induced plur15

Fibroblasts18

Neurons18

Somatic cell11

Progenitor cells7

Vitro9

Genes15

Neuronal1

Regenerative12

Therapeutic10

Cellular Reprogramming6

Methylation Reprogramming2

Fibroblast6

Pluripotency3

Vivo8

20233

Embryonic Stem6

Differentiated cells into pluripotent stem1

Embryos13

Bone marrow6

Glial3

Endogenous2

Harvard Stem Cell Institute2

Human adult1

Transplantation9

Brain6

Transcription7

Protocols4

Biology5

Nucleus4

Cardiac2

Genetically2

Functional4

Tumors4

Genetic9

Pluripotent embryonic1

GABAergic2

Protein8

Involves2

Dopaminergic1

Proliferation1

Tissues2

Successfully3

Researchers16

Mechanisms3

Scientists8

Nuclear3

Factors7

Developmental5

Metabolic4

Approaches2

Immunotherapy2

Similar to embryonic stem1

Skin cells7

IPSCs2

Antitumor1

Eliminate2