• They are large "platform" or "hub" proteins (c.2500 amino acids) that assemble a complex containing a number of histone deacetylase enzymes. (esrf.fr)
  • We mapped the regions in the three proteins required for interaction and demonstrated that the amino-terminal domain of TBL1 (TBL1-NTD) is able to bind both GPS2 and SMRT forming a ternary complex. (esrf.fr)
  • Hence the tetrameric nature of the TBL1 suggests that the protein may serve as a scaffold for a multivalent chromatin-targeted repression machine that contains multiple co-repressor proteins and histone deacetylase enzymes. (esrf.fr)
  • The KAP1/ KRAB-AFP complex in turn recruits the heterochromatin protein 1 (HP1) family, and other chromatin modulating proteins, leading to transcriptional repression through heterochromatin formation. (embl.de)
  • Repression of transcriptional activity by heterologous KRAB domains present in zinc finger proteins. (embl.de)
  • The core repression complex involves the recruitment of three proteins, HDAC3, GPS2 and TBL1, to a highly conserved repression domain within SMRT and NCoR. (kent.ac.uk)
  • While transcription factors bind and recruit chromatin-modifying and remodeling proteins, the relative contribution of individual cis elements residing within clusters of cis elements to the transcriptional control of endogenous loci is incompletely understood. (prolekarniky.cz)
  • The centromere proteins (CENPs), a critical mitosis-related protein complexes, are involved in the kinetochore assembly and chromosome segregation. (bvsalud.org)
  • The plant homeodomain (PHD) finger is a C4HC3 zinc-finger-like motif found in nuclear proteins thought to be involved in epigenetics and chromatin-mediated transcriptional regulation. (embl-heidelberg.de)
  • The aim of this thesis is to quantitatively and structurally describe the interactions that occur between DNA-bound proteins that regulate transcription, the DNA distortions brought about by them, and the putative role of DNA conformation on the regulation of transcriptional initiation. (yu.edu)
  • These results demonstrate that the interactions between the proteins in a multicomponent nucleoprotein complex cannot be described by simple pairwise cooperativity models. (yu.edu)
  • HDACs function by deacetylating of key components of the transcriptional machinery including the core histone proteins resulting in their in re-association with the DNA, thus presenting a transcriptionally closed conformation [ 1 , 16 ]. (biomedcentral.com)
  • To explore the possibility that SLK1 and SLK2 may serve as adaptor proteins to aid the interaction between LUH and DNA binding transcription factors, as seen for the SEU LUG complex, SLK1 BD or SLK2 BD DNA Inhibitors,Modulators,Libraries was co transfected with the CaMV 35S LUH or CaMV 35S LUFS Inhibitors,Modulators,Libraries construct, and effects on reporter expression were quanti tated. (aurorapathway.com)
  • Aberrant may methy lation of promoter CpG islands results in transcriptional silencing through several mechanisms, including the at traction of proteins that interact with histone deacetylases, and chromatin condensation, precluding the binding of transcriptional factors to the promoter, thus modulating gene expression and, consequently, tumour phenotype. (aurorapathway.com)
  • By screening for proteins that colocalize with NHR-67 punctae, we identified new regulators of uterine cell fate maintenance: homologs of the transcriptional co-repressor Groucho (UNC-37 and LSY-22), as well as the TCF/LEF homolog POP-1. (elifesciences.org)
  • A key element of stem cell maintenance is repression of differentiation factors or developmental genes - achieved transcriptionally, epigenetically by the Polycomb complex, and post-transcriptionally by RNA-binding proteins and microRNAs. (youbookinc.com)
  • Unexpectedly, the enzymatic activity of HDACs in these complexes has been shown to be regulated by inositol phosphates, which bind in a pocket sandwiched between the HDAC and co-repressor proteins. (ox.ac.uk)
  • How does the Tup1-Ssn6 repressor obtain alerts from a number of signaling pathways, combine people signals, and regulate gene expression appropriately The routines of Tup1 recruiting proteins are modulated by signaling pathways that are activated by an overlapping established of environmental situations and mobile stresses. (dna-alkylating.com)
  • Years of operate from numerous labs studying Tup1 and its recruiters together with our outcomes propose that Tup1, Ssn6, and a number of recruiting proteins form a repressor complex that prevents the expression of hundreds of genes that are not necessary beneath normal laboratory growth problems [six,18,51]. (dna-alkylating.com)
  • Besides classical gene regulation, miRNAs have emerged as post-transcriptional regulators of angiogenesis. (plos.org)
  • Tumor-infiltrated macrophages were isolated from human GC tissues using magnetic beads, gene transcription was determined by real-time PCR, protein expression was monitored using western blots, metabolites were determined using HPLC, and transcriptional regulation was analyzed by the luciferase-based reporter gene system. (cancerindex.org)
  • It is not understood whether these elements function collectively to confer transcriptional regulation, or individually to control specific aspects of activation or repression, such as initiation versus maintenance. (prolekarniky.cz)
  • Models of gene regulation have led to an attractive paradigm in which repression occurs in sequential stages of increasing stability [4] . (prolekarniky.cz)
  • GATA factor cross-regulation represents an instructive model system for investigating the contribution of individual cis elements to the initiation and maintenance of transcriptional repression. (prolekarniky.cz)
  • Essentiality of the Maltase AmlE in Maltose Utilization and Its Transcriptional Regulation by the Repressor AmlR in the Acarbose-Producing Bacterium Actinoplanes sp. (frontiersin.org)
  • The repression and activation of transcriptional regulation require a complex interplay of protein-DNA and protein-protein interactions. (yu.edu)
  • Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. (affbiotech.cn)
  • Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. (affbiotech.cn)
  • The key co-repressor complex components HDAC-2, Mi-2α/β and mSin3a are all critical to the regulation of gene transcription. (biomedcentral.com)
  • We propose a model in which association of NHR-67 with the Groucho/TCF complex suppresses the default invasive state in non-invasive cells, which complements transcriptional regulation to add robustness to the proliferative-invasive cellular switch in vivo . (elifesciences.org)
  • This valuable data study presents convincing data that expression of the C. elegans transcription factor NHR-67 is sufficient to drive an invasive fate, and that the alternative proliferative fate is associated with NHR-67 transcriptional down-regulation. (elifesciences.org)
  • This volume describes the latest findings on transcriptional and translational regulation of stem cells. (youbookinc.com)
  • This is due to concomitant relief of carbon catabolite repression (CCR) and activation of mitochondrial retrograde signaling, a mitochondria-to-nucleus communication pathway causing up-regulation of various nuclear target genes, such as CIT2 , encoding peroxisomal citrate synthase, dependent on the positive regulator RTG2 in response to mitochondrial dysfunction. (microbialcell.com)
  • Transcriptional regulation of the genes in metabolic pathways is a highly successful strategy, which is virtually universal in microorganisms. (lu.se)
  • With a minimalist model of metabolism, cell growth and transcriptional regulation in a microorganism, we explore how the interaction between environmental conditions and gene regulation set the growth rate of cells in the phase of exponential growth. (lu.se)
  • Citation: Troein C, Ahre´n D, Krogh M, Peterson C (2007) Is Transcriptional Regulation of Metabolic Pathways an Optimal Strategy for Fitness? (lu.se)
  • INTRODUCTION broken, which in realistic situations can severly constrain the Transcriptional regulation of effector genes is a highly successful regulatory options. (lu.se)
  • These data predict that the ligand-induced switch of heterodimeric nuclear receptors from repressor to activator functions involves the exchange of complexes containing histone deacetylases with those that have histone acetylase activity. (fhcrc.org)
  • Overall, we find that E2a functions as both a transcriptional repressor and activator to precisely regulate Nodal signaling. (xenbase.org)
  • For this purpose, a thermodynamic analysis of the interactions that occur upon the binding of repressor, activator and RNAP was performed using quantitative DNase I footprinting. (yu.edu)
  • Here we demonstrate that dCtBP is dispensable for target enhancers that contain overlapping activator and repressor binding sites. (princeton.edu)
  • However, it is essential when Krüppel and Knirps repressor sites do not overlap activator sites but are instead located adjacent to either activators or the core promoter. (princeton.edu)
  • most of its (hemi-)cellulolytic enzymes are obligatorily dependent on the transcriptional activator xyr1. (liverpool.ac.uk)
  • In contrast, the Hog kinase pathway regulates Sko1 by changing it from a repressor to an activator [forty eight]. (dna-alkylating.com)
  • The KRAB domain functions as a transcriptional repressor when tethered to the template DNA by a DNA-binding domain. (embl.de)
  • In contrast, LUH BD significantly reduced reporter gene expression in a concentration dependent manner, indicating that LUH functions as a transcriptional repressor in vivo. (aurorapathway.com)
  • E2a is necessary for Smad2 /3-dependent transcription and the direct repression of lefty during gastrulation. (xenbase.org)
  • E2a changes the position of Smad2 /3 binding at the Nodal inhibitor lefty , resulting in direct repression of lefty that is critical for mesendoderm specification. (xenbase.org)
  • First, the indirect repression would lead to the propagation of metabolic burden and noise through networks comparing with direct repression. (igem.org)
  • The second is direct repression, in which repressors block the function of the core transcription complex. (princeton.edu)
  • These findings provide evidence that competition is distinct from quenching and direct repression. (princeton.edu)
  • Quenching and direct repression depend on dCtBP, whereas competition does not. (princeton.edu)
  • RBPJ can function as a coactivator when Notch signaling is activated but acts as a repressor in the absence of a Notch stimulus. (mdpi.com)
  • At a genome-wide level, SAMD1 localizes to H3K4me3-decorated CGIs, where it acts as a repressor. (ox.ac.uk)
  • Evidence from model systems shows that MeCP2 can recruit the Sin3a co-repressor complex to promoters leading to transcriptional repression, therefore suggesting that MeCP2 can interpret the DNA methylation signal to bring about gene silencing. (portlandpress.com)
  • The functions currently known for members of the KRAB-containing protein family include transcriptional repression of RNA polymerase I, II and III promoters, binding and splicing of RNA, and control of nucleolus function. (embl.de)
  • Eukaryotic transcriptional repressors function by recruiting large coregulatory complexes that target histone deacetylase enzymes to gene promoters and enhancers. (kent.ac.uk)
  • Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: Coupling protein O-GlcNAcylation to transcriptional repressor. (exalpha.com)
  • Transcriptional repression complexes play important roles in both repressing gene transcription and in "resetting" genes after rounds of active transcription. (esrf.fr)
  • YY1 is known to regulate transcriptional activation and repression of various genes associated with different cellular processes such as DNA repair, autophagy, cell survival and apoptosis, and cell division. (bvsalud.org)
  • Although AmlR apparently is a local transcriptional regulator of the aml operon, the Δ amlR strain shows severe growth inhibitions on glucose and - concomitantly - differential transcription of several genes of various functional classes. (frontiersin.org)
  • TUP 1 and SSN6 are involved in repression of several diverse families of genes in yeast, including cell type-specific genes regulated by the alphga2 and a1/alpha2 repressors. (exalpha.com)
  • In both human and murine lymphomas, CREBBP loss-of-function resulted in focal depletion of enhancer H3K27 acetylation and aberrant transcriptional silencing of genes that regulate B-cell signaling and immune responses, including class II MHC. (elsevierpure.com)
  • HDAC3 loss-of-function rescued repression of these enhancers and corresponding genes, including MHC class II, and more profoundly suppressed CREBBP -mutant lymphomas in vitro and in vivo. (elsevierpure.com)
  • SIGNIFICANCE: Our fi ndings establish the tumor suppressor function of CREBBP in GC lymphomas in which CREBBP mutations disable acetylation and result in unopposed deacetylation by BCL6/SMRT/ HDAC3 complexes at enhancers of B-cell signaling and immune response genes. (elsevierpure.com)
  • Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon. (yeastgenome.org)
  • Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. (abcam.cn)
  • Co repressors Inhibitors,Modulators,Libraries in the Gro/Tup1 family mediate repres sion by recruiting histone deacetylases to the target genes. (aurorapathway.com)
  • While the observation that NHR-67 forms punctae associated with transcriptional repressors in non-invasive cells is intriguing, the work does not yet established a clear link between the formation and dissolution of NHR-67 condensates with the activation of downstream genes that NHR-67 is actively repressing. (elifesciences.org)
  • One such wide-domain regulatory circuit, essential to all cells, is carbon catabolite repression (CCR): it allows the cell to prefer some carbon sources, whose assimilation is of high nutritional value, over less profitable ones. (biomedcentral.com)
  • One such wide-domain regulatory circuit is carbon catabolite repression (CCR): it allows the preferred assimilation of carbon sources of high nutritional value over others [ 1 - 4 ]. (biomedcentral.com)
  • We report that chick Dach1 interacts with the Smad complex and the corepressor mouse Sin3a, thereby acting as a repressor of BMP-mediated transcriptional control. (biologists.com)
  • Furthermore, the recruitment of an HDAC/Sin3A complex to Nkx3.2 requires that Nkx3.2 interact with Smad1 and -4. (elsevierpure.com)
  • Indeed, Nkx3.2 both fails to associate with the HDAC/Sin3A complex and represses target gene transcription in a cell line lacking Smad4, but it performs these functions if exogenous Smad4 is added to these cells. (elsevierpure.com)
  • In summary, CENPA function as a transcriptional regulator to promote HCC via cooperating with YY1. (bvsalud.org)
  • in an operon with an upstream PurR/LacI-type transcriptional regulator gene, named amlR ( ACSP50_2475 ), and a gene downstream ( ACSP50_2473 ) encoding a GGDEF-EAL-domain-containing protein putatively involved in c-di-GMP signaling. (frontiersin.org)
  • B) When AHL presents and binds to transcriptional regulator, the transcription regulator binds its DNA binding site, thus to reduce the accessibility of RNA polymerase to promoter, repressing the transcription initiation. (igem.org)
  • the ß-importin kap8 (pse1/kap121) is required for nuclear import of the cellulase transcriptional regulator xyr1, asexual sporulation and stress resistance in trichoderma reesei. (liverpool.ac.uk)
  • Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
  • The EZH2 gene encodes for an enzyme that is involved in histone methylation and transcriptional repression. (bvsalud.org)
  • The conserved "core" of the SMRT/NCoR repression complexes (called repression domain 1 or RD1) recruits HDAC3, TBL1 (and/or its homologue TBLR1) as well as a protein called GPS2 (also known as AMF1). (esrf.fr)
  • MeCP2 binds methylated DNA via its methyl-CpG binding domain (MBD) and recruits co-repressor complexes via its transcriptional repression domain. (berkeley.edu)
  • Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. (abcam.cn)
  • The first is quenching, whereby repressors and activators co-occupy closely linked sites and then the repressor inhibits adjacent activators. (princeton.edu)
  • Binding of ferrous iron to the DtxR molecule forms a complex that binds to the tox gene operator and inhibits transcription. (medscape.com)
  • AEBP2 may regulate the migration and development of the neural crest cells through the PRC2-mediated epigenetic mechanism and is most likely a targeting protein for the mammalian PRC2 complex. (wikipedia.org)
  • This thesis suggests that transcriptional activation and repression cannot be studied as independent mechanisms, and provides a first step toward understanding the interplay of DNA structure, protein-protein and protein-DNA interactions, which together regulate the formation and efficiency of a transcription initiation complex. (yu.edu)
  • Recent advances in understanding the role that epigenetics plays in cancer pathogenesis and understanding the mechanisms through which these processes regulate gene expression have stimulated considerable interest in developing clinically viable antineoplastic agents that target enzymatic components of transcriptional regulatory complexes responsible for the establishment of pathologic epigenetic modifications that lead to deregulated gene expression in cancer. (aacrjournals.org)
  • Mechanistically, CREBBP-regulated enhancers are counter-regulated by the BCL6 transcriptional repressor in a complex with SMRT and HDAC3, which we found to bind extensively to MHC class II loci. (elsevierpure.com)
  • Hence, CREBBP loss-of-function contributes to lymphomagenesis by enabling unopposed suppression of enhancers by BCL6/SMRT/HDAC3 complexes, suggesting HDAC3-targeted therapy as a precision approach for CREBBP -mutant lymphomas. (elsevierpure.com)
  • It is a subunit of a Polycomb repressor complex 2 (PRC2). (bvsalud.org)
  • Histone deacetylases (HDACs) 1, 2 and 3 form the catalytic subunit of several large transcriptional repression complexes. (ox.ac.uk)
  • These structures, together with computational docking, mutagenesis and functional assays, reveal the assembly mechanism and stoichiometry of the corepressor complex. (kent.ac.uk)
  • A complex has been identified that contains N-CoR, the Mad presumptive co-repressor mSin3, and the histone deacetylase mRPD3, and which is required for both nuclear receptor- and Mad-dependent repression, but not for repression by transcription factors of the ets-domain family. (fhcrc.org)
  • Gene transcription is tightly regulated by a highly complex and dynamic set of processes central to which is the recruitment of co-repressors to promoter bound sequence specific transcription factors [ 1 - 3 ]. (biomedcentral.com)
  • Advances in molecular biology and genome analysis now also allow for detailed descriptions of DNA-binding transcription factors and transcriptional regulatory networks. (medscape.com)
  • We report that p107 and p130 formed transcriptional complexes with E2F4 on the FGFR1 promoter and repressed FGFR1 gene transcription in myogenic cells. (wikigenes.org)
  • YY1 promoted its transcriptional expression by directly binding to the MDR1 promoter. (bvsalud.org)
  • Therefore, a switch that produces a negative feed back, an inverter in another word, was conventionally implemented by linking an input promoter to the expression of a repressor, which then turns off a downstream promoter generally, using the repressor-operator pairs as shown in Fig 1. (igem.org)
  • AEBP2 is a potential targeting protein for the mammalian Polycomb Repression Complex 2 (PRC2). (wikipedia.org)
  • In lower multicellular fungi, the C2H2 zinc finger CreA/CRE1 protein has been shown to act as the transcriptional repressor in this process. (biomedcentral.com)
  • A key mechanism appears to be the following: KRAB-AFPs tethered to DNA recruit, via their KRAB domain, the repressor KAP1 (KRAB-associated protein-1, also known as transcription intermediary factor 1 beta, KRAB-A interacting protein and tripartite motif protein 28). (embl.de)
  • The function of this domain is not yet known but in analogy with the LIM domain it could be involved in protein-protein interaction and be important for the assembly or activity of multicomponent complexes involved in transcriptional activation or repression. (embl-heidelberg.de)
  • Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. (cusabio.com)
  • The Rb protein regulates differentiation, apoptosis, and cell cycle control by coordinating the cell cycle at G1-S with transcriptional machinery. (affbiotech.com)
  • The impact of oxidative stress on key components of the co-repressor complexes have only just started to be explored, with the very recent publication highlighting the impact of oxidative stress driven protein kinase-CK2 activation on co-repressor activity and HDAC2 function [ 20 ]. (biomedcentral.com)
  • We have previously shown that Nkx3.2, a transcriptional repressor that is expressed in the sclerotome and developing cartilage, can activate the chondrocyte differentiation program in somitic mesoderm in a bone morphogenetic protein (BMP)-dependent manner. (elsevierpure.com)
  • The third is competition, in which repressors compete with activators for a common DNA-binding site. (princeton.edu)
  • Both transcriptional activators and repressors have been shown to be crucial for the maintenance of the stem cell state. (youbookinc.com)
  • Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. (affbiotech.cn)
  • In this work, we investigated the relationships between the RTG and CCR pathways in the modulation of AA-PCD sensitivity under glucose repression or de-repression conditions. (microbialcell.com)
  • While prior work has indicated that BMP-dependent Smads can support transcriptional activation, our findings indicate that BMP-dependent Smads can also potentiate transcriptional repression, depending upon the identity of the Smad-interacting transcription factor. (elsevierpure.com)
  • Our findings demonstrate that the −1.8 kb site selectively maintains repression, confers a specific histone modification pattern and expels RNA Polymerase II from the locus. (prolekarniky.cz)
  • The existence of a macromolecular repression complex that potentially includes Gal repressor (GalR), RNAP polymerase (RNAP) and the cAMP-CAP complex was investigated. (yu.edu)
  • Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. (cusabio.com)
  • 3) the binding of LacI or GalR to the {dollar}O\sb{lcub}E{rcub}{dollar} site, just upstream of the CAP binding site, modulates the CAP-RNAP cooperativity, which might be a part of the mechanism of repression. (yu.edu)
  • here, we investigated the nucleo-cytoplasmic shuttling mechanism that transports xyr1 across the nuclear pore complex. (liverpool.ac.uk)
  • SAMD1 tethers L3MBTL3 to chromatin and interacts with the KDM1A histone demethylase complex to modulate H3K4me2 and H3K4me3 levels at CGIs, thereby providing a mechanism for SAMD1-mediated transcriptional repression. (ox.ac.uk)
  • Insights into the activation mechanism of class I HDAC complexes by inositol phosphates. (ox.ac.uk)
  • The crystal structure of the HDAC1:MTA1 complex bound to a novel peptide-based inhibitor and to inositol hexaphosphate suggests a molecular basis of substrate recognition, and an entropically driven allosteric mechanism of activation. (ox.ac.uk)
  • Both positive and negative acting marks are established across pro moters during gene activation or gene repression, respect ively, and the interplay of those histone modifications ultimately control gene expression. (aurorapathway.com)
  • In addition, we found CRE1-repression of nitrogenous substances uptake, components of chromatin remodeling and the transcriptional mediator complex, as well as developmental processes. (biomedcentral.com)
  • The work will be of interest to developmental biologists studying transcriptional control of cell fate specification in animals, especially once issues around the functional significance of the NHR-67 contiaining punctae are resolved. (elifesciences.org)
  • These stimulations activate a broad range of signaling cascades, such as canonical and non-canonical NF- κ B pathways, mitogen-activated kinase (MAPK) pathways and calcium signaling, which in turn activate downstream transcriptional regulators to drive osteoclastogenesis. (frontiersin.org)
  • It may interact with and stimulate the activity of the PRC2 complex. (wikipedia.org)
  • We also showed that each dimer of TBL1 is able to bind a heterodimer of SMRT and GPS2 and thus the physiological relevant stoichiomentry of the TBL1:SMRT:GPS2:HDAC3 complex is 4:2:2:2. (esrf.fr)
  • Taken together, the crystal structure of the TBL1-NTD tetramer, the NMR structure of the SMRT:GPS2 heterodimer, along with mutagenesis and docking experiments give us considerable insight into the assembly of the core SMRT/NCoR:TBL1:GPS2:HDAC3 repression complex. (esrf.fr)
  • SMRT, GPS2 and TBL1 form a ternary complex in a three-way interaction. (esrf.fr)
  • Adjacent interaction regions in SMRT and GPS2 form a binary complex. (esrf.fr)
  • We were unable to obtain crystals of the SMRT:TBL1:GPS2 ternary complex. (esrf.fr)
  • However, using NMR spectroscopy we were able to determine the structure of the binary complex between SMRT and GPS2. (esrf.fr)
  • The heterodimerisation of SMRT and GPS2 allows them to form a higher affinity complex with TBL1 than could be formed with either alone. (esrf.fr)
  • Fig. 103: Schematic of the overall arrangement of the SMRT repression complex, a multivalent chromatin deacetylating machine. (esrf.fr)
  • Transcriptional repression complexes, assembled by the corepressor NCoR and its homolog SMRT, are crucial in many processes, including development and metabolic physiology. (kent.ac.uk)
  • We report the crystal structure of the tetrameric oligomerization domain of TBL1, which interacts with both SMRT and GPS2, and the NMR structure of the interface complex between GPS2 and SMRT. (kent.ac.uk)
  • Although the latter is predominantly associated with the NuRD complex they are structurally similar and no functional or cell type specific differentiation between Mi-2α and Mi-2β has yet been made [ 6 ]. (biomedcentral.com)
  • the velvet complex in the gray mold fungus botrytis cinerea: impact of bclae1 on differentiation, secondary metabolism, and virulence. (liverpool.ac.uk)
  • natural variation in bcvel1 encoding the ortholog of aspergillus nidulans vea, a member of the velvet complex, was previously shown to affect light-dependent differentiation, the formation of oxalic acid (oa), and virulence. (liverpool.ac.uk)
  • Yeast Tup 1p-Ssn6p repressor complex represents a novel paradigm for transcriptional repression and for the role of chromatin in repression. (exalpha.com)
  • We found that the pro-angiogenic effects are partly mediated through reduced repression by miR-101 of the histone-methyltransferase EZH2, a member of the Polycomb group family, thereby increasing methylation of histone H3 at lysine 27 and transcriptome alterations. (plos.org)
  • Ssn6-Tup1 interacts with class I histone deacetylase required for repression. (exalpha.com)
  • Steady with this observation, we have shown earlier that Tup1 continues to be certain to many targets even when transcriptional repression is relieved [seventeen]. (dna-alkylating.com)
  • Transcription of the aml operon is significantly repressed in the wild type when growing on glucose and repression is absent in an Δ amlR deletion mutant. (frontiersin.org)
  • In the presence of ferrous iron, the DtxR-iron complex attaches to the tox gene operon, inhibiting transcription. (medscape.com)
  • The covalent modification of histones to yield specific histone marks promotes either the activation or repression of transcription [3] . (prolekarniky.cz)
  • This complex would insure that upon activation, RNAP would be poised to immediately start the process of transcriptional initiation. (yu.edu)
  • The communication between the mechanisms of activation and repression occurs by two different mechanisms. (yu.edu)
  • All of the quorum sensing systems currently exploited in synthetic biology exhibit transcriptional activation, which cannot provide negative feed back loops during cell-cell communication. (igem.org)
  • Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner (By similarity). (affbiotech.cn)
  • During the activation phase, promotes the accumulation of ubiquitinated ARNTL/BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and ARNTL/BMAL1 (By similarity). (affbiotech.cn)
  • The identification and characterization of the transcriptional regulatory networks governing the physiology and adaptation of microbial cells is a key step in understanding their behaviour. (biomedcentral.com)
  • Transcriptional repression by nuclear receptors has been correlated to binding of the putative co-repressor, N-CoR. (fhcrc.org)
  • There a luxR dimer could behave as a repressor when binding to its cognate effector molecule, AHL (acetyl-Homoserine Lactone). (igem.org)
  • Ssn6-Tupl interacts with class I histone deacetylases required for repression, and rfecrfuitment of yeast Tuplp-Ssn6p repressor is associated with localized decreases in histone acetylation. (exalpha.com)
  • 1. Bone, J.R. and Roth, S.Y. 'Recruitment of the yeast Tup1p-Ssn6p repressor is associated with localized decreases in histone acetylation. (exalpha.com)
  • Metazoan development is characterized by complex transcriptional programs specified by gene regulatory networks [1] , [2] . (prolekarniky.cz)
  • Transcription factor complexes have varied effects on cell fate and behavior, but how this diversification of function occurs is largely unknown. (xenbase.org)
  • Previous studies have shown that the Drosophila melanogaster CtBP corepressor (dCtBP) is essential for the quenching activity of three short-range sequence-specific repressors in the early Drosophila embryo: Krüppel, Knirps, and Snail. (princeton.edu)
  • Previous studies have shown that the Drosophila melanogaster CtBP corepressor (dCtBP) is essential for the quenching activity of three short-range sequence-specific repressors in the early Drosophila embryo: Kr{\"u}ppel, Knirps, and Snail. (princeton.edu)
  • increases YY1 repression activity. (affbiotech.cn)
  • Wild type, PI3Kγ knock-out (PI3Kγ -/- ) and PI3K kinase dead knock-in (PI3Kδ D910/A910 ) transgenic mice were exposed to cigarette smoke for 3 days and the expression levels of the co-repressor complex components HDAC-2, mSin3a, Mi-2α and Mi-2β and HDAC-2 activity in the lungs were assessed. (biomedcentral.com)
  • Cigarette smoke exposure induced glucocorticoid insensitivity and alters co-repressor activity and expression which is prevented by blockade of PI3K signaling with glucocorticoid treatment. (biomedcentral.com)
  • LUH has repressor activity To determine whether LUH, SLK1 and SLK2 could func tion as transcriptional repressors, a Inhibitors,Modulators,Libraries repression assay in Arabidopsis protoplasts was performed. (aurorapathway.com)
  • In this work, we elucidate how BMP signaling modulates the transcriptional repressor activity of Nkx3.2. (elsevierpure.com)
  • The homeodomain and NK domain of Nkx3.2 support the interaction of this transcription factor with HDAC1 and Smad1, respectively, and both of these domains are required for the transcriptional repressor activity of Nkx3.2. (elsevierpure.com)
  • It can be anticipated that the absence of feedback mechanisms in Boolean or Non-Boolean gene networks is definitely a major hurdle towards complex behaivor for synthetic microbial consortia. (igem.org)
  • There are three mechanisms of transcriptional repression in eukaryotes. (princeton.edu)
  • Moreover, SLK1 and SLK2 interaction with the LUFS do main were as strong as full length LUH supporting the idea that the LUFS domain is sufficient for interaction with SLK1 and SLK2 to form LUH SLK1 and LUH SLK2 co repressor complexes in vivo. (aurorapathway.com)
  • We have found that Nkx3.2 forms a complex, in vivo, with histone deacetylase 1 (HDAC1) and Smadl and -4 in a BMP-dependent manner. (elsevierpure.com)
  • We engineered mice lacking a single cis element −1.8 kb upstream of the Gata2 transcriptional start site. (prolekarniky.cz)
  • Both the mSin3a and Mi-2/NuRD co-repressor complexes are large multi-component complexes in which not all of the components and their functions have been identified [ 2 , 6 ]. (biomedcentral.com)
  • A sequence of 45 amino acids in the KRAB A subdomain has been shown to be necessary and sufficient for transcriptional repression. (embl.de)
  • Even the crudest transcriptional network is shown to substantially increase the fitness of the organism, and this effect persists even when the range of nutrient levels is kept very narrow. (lu.se)
  • Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor. (exalpha.com)