AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesTechnology, Industry, AgricultureInformation Science
Silencer Elements, TranscriptionalRegulatory Sequences, Nucleic AcidBase SequencePromoter Regions, GeneticEnhancer Elements, GeneticMolecular Sequence DataGene SilencingRepressor ProteinsTranscription, GeneticGenes, RegulatorDNA-Binding ProteinsTranscription FactorsGene Expression RegulationGenes, Mating Type, FungalIntronsHeLa CellsBinding SitesDNA FootprintingExonsTransfectionDNANuclear ProteinsChloramphenicol O-AcetyltransferaseGenes, ReporterDNA Transposable ElementsSequence Homology, Nucleic AcidSequence DeletionPlasmidsResponse ElementsVimentinCell LineAlternative SplicingConsensus SequenceSp1 Transcription FactorTumor Cells, CulturedRNA, MessengerProtein BindingRNA SplicingSilent Information Regulator Proteins, Saccharomyces cerevisiaeDNA PrimersChickensL Cells (Cell Line)Zinc FingersOligodeoxyribonucleotidesMutationCell NucleusMutagenesis, Site-DirectedHeterogeneous-Nuclear Ribonucleoprotein Group A-BRecombinant Fusion ProteinsNeuronsMice, TransgenicPolymerase Chain ReactionAlu ElementsRecombinant ProteinsRegulatory Elements, TranscriptionalTrace ElementsFinite Element AnalysisRegulatory Sequences, Ribonucleic AcidElementsInsulator ElementsSaccharomyces cerevisiaeRepetitive Sequences, Nucleic AcidGenes, FungalYY1 Transcription FactorModels, GeneticLong Interspersed Nucleotide ElementsRNA Splice SitesDeoxyribonuclease IAmino Acid SequenceRestriction MappingGene Expression Regulation, FungalCloning, MolecularShort Interspersed Nucleotide ElementsRetroelementsTrans-ActivatorsDNA, FungalTranscriptional ActivationChromatinGlobinsConserved SequenceHeterogeneous-Nuclear RibonucleoproteinsNucleic Acid ConformationRNA-Binding ProteinsGene Expression Regulation, EnzymologicLuciferasesChromosomes, FungalErythroid-Specific DNA-Binding FactorsSirtuin 2Drosophila melanogasterRNA, Transfer, ThrSequence Analysis, DNAElectrophoretic Mobility Shift AssayFungal ProteinsDrosophilaOrigin Recognition ComplexMutagenesis
RegulationRepressorRepressionGenesChromatinRegulatorPost-transcriptionalRegulatePromoterProteinsRegulatory elementsMammalianSequencesYeastSequenceGenomicHomologousEndogenousHistoneTransposable elementsUpstreamMolecularFunctionsCancerFactorsActivationRecognizeHumanMultipleTargetResponse elementsShort
Regulation6
  • MicroRNAs perform important roles in the post-transcriptional regulation of gene expression. (researchsquare.com)
  • The results suggest that their interactions are involved in renin transcriptional regulation. (biomedcentral.com)
  • As splicing is linked to transcriptional and post-transcriptional steps, SR proteins are implicated in the regulation of multiple aspects of the gene expression program. (molcells.org)
  • This important study advances our understanding of the functions and regulation of the Drosophila transcriptional regulator Bonus, an ortholog of mammalian TIF1 family members. (elifesciences.org)
  • The purpose of this study was to clarify the roles of paraspeckles in PXR-mediated transcriptional regulation. (aspetjournals.org)
  • Transposable elements are able to affect conserved and divergent chromatin looping and contribute to cell- and species-specific gene regulation [ 11 ]. (biomedcentral.com)
Repressor5
  • RE1-Silencing Transcription factor (REST), also known as Neuron-Restrictive Silencer Factor (NRSF), is a protein which in humans is encoded by the REST gene, and acts as a transcriptional repressor. (wikipedia.org)
  • This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. (wikipedia.org)
  • The forming of biofilm neighborhoods is crucial for survival and persistence in character as well such as disease transmitting [5 9 The histone-like nucleoid structuring proteins (H-NS) is certainly a nucleoid linked DAPK Substrate Peptide proteins and transcriptional repressor [12]. (healthcarecoremeasures.com)
  • Nef protein of HIV-1 is a transcriptional repressor of HIV-1 LTR. (microbiologyresearch.org)
  • Solid evidence, leveraging both in vivo and in vitro studies, reveals the molecular requirements for Bonus' function as a transcriptional repressor, including a key role for sumoylation. (elifesciences.org)
Repression5
  • Repression by H-NS could be relieved in response to environmental cues DAPK Substrate Peptide that activate the appearance of various other regulators whose binding site overlaps that of H-NS [13 21 For instance transcriptional silencing of and promoters by H-NS is certainly antagonized with the AraC-like transcriptional regulator ToxT as well as the IHF [22-24]. (healthcarecoremeasures.com)
  • Rasd1 inhibited transcriptional repression by Ear2 on a renin promoter-luciferase reporter construct both in the presence and absence of all-trans-retinoic acid. (biomedcentral.com)
  • The assays found tissue-specific enhancer elements as well as cis -regulatory elements that are required for the maintenance of activation or repression throughout development. (nih.gov)
  • Characterization of the chromosomal rearrangements shown in Figure 1 also revealed that two genetic elements (proximal and distal MES [maintenance elements for silencing]), about 70 kb apart in the Scr gene, must be in cis to maintain proper repression. (nih.gov)
  • Together, our work identified Bonus as a regulator of tissue-specific gene expression and revealed the importance of SUMOylation as a regulator of complex formation in the context of transcriptional repression. (elifesciences.org)
Genes12
  • It is assumed that REST represses genes that promote cell death and Alzheimer's disease pathology, and induces the expression of stress response genes. (wikipedia.org)
  • The RNA-seq evaluation identified brand-new genes straight repressed by H-NS that may influence virulence and biofilm advancement in the Un Tor biotype cholera bacterium. (healthcarecoremeasures.com)
  • Assays in transgenes in Drosophila previously identified cis -acting transcriptional regulatory elements from homeotic genes. (nih.gov)
  • While these transgenic assays have been important in defining the structure of the cis -regulatory elements and identifying trans -acting factors that bind to them, their functions within the context of the endogenous genes remain poorly understood. (nih.gov)
  • The transcriptional silencers are clustered in the two regions whose interactions are required for the maintenance of silencing in the endogenous genes. (nih.gov)
  • In Drosophila , the BX-C cluster contains the three most posterior Hox genes, where their collinear activation incorporates progressive replacement of histone modifications, reorganization of 3D chromatin architecture and sequential activation of boundary elements and cis -regulatory regions. (biorxiv.org)
  • The collinear activation of the Hox genes and their cis -regulatory elements is thought to rely on the progressive opening of the chromatin within the BX-C along the A-P axis. (biorxiv.org)
  • The conserved family of Transcription Intermediary Factors (TIF1) proteins consists of key transcriptional regulators that control transcription of target genes by modulating chromatin state. (elifesciences.org)
  • We found that knockdown of Bonus in early oogenesis results in severe defects in ovarian development and in ectopic expression of genes that are normally repressed in the germline, demonstrating its essential function in the ovary. (elifesciences.org)
  • Some genes lack a TATA box and have an initiator element instead. (stemcelldaily.com)
  • As is recognized, Wnt11 is actually certainly one of several B catenin TCF target genes that con tain adjacent putative Kaiso and TCF LEF binding websites within their promoter, suggesting that Kaiso and TCF LEF cooper ate to repress Wnt11transcription. (screeninglibraries.com)
  • Transposable elements can be considered as a source of raw material for primitive genomes, tools of genetic innovation, and ancestors of modern genes (e.g., ncRNA) [ 15 ]. (biomedcentral.com)
Chromatin4
  • In the repressed state, the PRC-binding regions are in close proximity, while the intervening chromatin harbouring ARF loops out. (biomedcentral.com)
  • Our results revealed a chromatin looping mechanism of long-range control and argue against models involving homogeneous spreading of PcG silencers across the INK4b-ARF-INK4a locus. (biomedcentral.com)
  • This chromatin organization differentially instructed alternative Abd-B promoter use, thereby expanding the possibilities to regulate transcriptional output. (biorxiv.org)
  • Transposable elements (TE) are important components of livestock and poultry genomes, contributing to their genetic diversity, chromatin states, gene regulatory networks, and complex traits of economic value. (biomedcentral.com)
Regulator2
  • Accepted August 23, 2012 ABSTRACT The catabolite control protein CcpA is a pleiotropic regulator that mediates the global transcriptional response to rapidly catabolizable carbohydrates, like glucose in Gram-positive bacteria. (studyres.com)
  • Methyl CpG Binding Protein 2 (MeCP2) is a key transcriptional regulator in the brain [ 1 ]. (biomedcentral.com)
Post-transcriptional2
  • A number of post-transcriptional mechanisms fine-tune the gene expression landscape. (researchsquare.com)
  • I will also discuss their emerging regulatory roles in mediating and connecting post-transcriptional processes from the nucleus to the cytoplasm. (molcells.org)
Regulate2
  • Current research is focused on the mechanisms that regulate SMAD activity to evoke cell-type-specific and context-dependent transcriptional programmes. (nature.com)
  • PcG silencers bind and coordinately regulate INK4b and INK4a , but not ARF , during a variety of physiological processes. (biomedcentral.com)
Promoter4
  • The most-extensively studied core promoter element in eukaryotes is a short DNA sequence known as a TATA box, found 25-30 base pairs upstream from the start site of transcription. (stemcelldaily.com)
  • In uninfected and cells immediately post-infection all three DHS sites were open, then the CD3γ promoter became non accessible, and this was followed by a sequential closure of all the DHS sites corresponding to all three transcriptional control regions. (biomedcentral.com)
  • Heat can affect their coming and going, and their presence near promoter elements can block gene activation. (anti-agingfirewalls.com)
  • In maize, a miniature inverted-repeat transposable element (MITE) inserted into the promoter of the NAC gene ( ZmNAC111 ) has been found to enhance drought tolerance at the seedling stage [ 20 ]. (biomedcentral.com)
Proteins1
  • The canonical TGFβ signalling pathway involves ligand-dependent assembly of a heteromeric receptor complex, receptor-kinase activation and subsequent phosphorylation and activation of SMAD proteins, which are transcriptional regulators that consequently accumulate in the nucleus. (nature.com)
Regulatory elements2
Mammalian1
  • Kaiso and prognosis As expected for a transcriptional aspect, the Kaiso Inhibitors,Modulators,Libraries protein is often discovered while in the nucleus of quite a few tumor or non tumor derived mammalian cell lines. (screeninglibraries.com)
Sequences1
  • The histone-like nucleoid structuring protein (H-NS) functions as a transcriptional silencer by binding to AT-rich sequences at bacterial promoters. (healthcarecoremeasures.com)
Yeast2
  • Characterization of a "silencer" in yeast: a DNA sequence with properties opposite to those of a transcriptional enhancer. (ox.ac.uk)
  • HDACs are grouped into three classes on the basis of their homology to yeast transcriptional repressors. (biomedcentral.com)
Sequence2
  • It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element (NRSE, also known as RE1). (wikipedia.org)
  • However since HMRE represses, rather than enhances, transcription we have called it a "silencer" sequence. (ox.ac.uk)
Genomic1
  • The transcription units are shown above the genomic DNA, while chromosomal aberrations are shown below (solid triangles indicate insertions of transposable elements and upward arrows indicate breakpoints of translocations and inversions). (nih.gov)
Homologous1
  • When not physically linked, the elements interact with elements on the homologous chromosome and cause derepression of its wild-type Scr gene. (nih.gov)
Endogenous1
  • We used a large number of existing chromosomal aberrations in the Scr homeotic gene to investigate the functions of the cis -acting elements within the endogenous gene. (nih.gov)
Histone1
  • Our work establishes the importance of the Fab-7 boundary element for insulation between 3D domains marked by different histone modifications. (biorxiv.org)
Transposable elements1
Upstream1
Molecular1
  • however, in the intervening years, a wealth of literature validated that they can be targeted indirectly through transcriptional co-activators, their confederates in various physiological and molecular processes. (bvsalud.org)
Functions1
  • ncRNAs contribute by far the majority of human transcriptional units, and the functions of the most are yet unknown. (lidsen.com)
Cancer1
  • TET2 is a potential cancer silencer quality with change rates going from 20% to 29% in SM. (md-fm.com)
Factors1
  • CcpA also directly represses key factors in early steps of sporulation (Spo0A and SigF). (studyres.com)
Activation1
Recognize1
  • We utilized an RNA-seq evaluation method of determine the foundation from the above phenotypes and recognize new goals of H-NS transcriptional silencing. (healthcarecoremeasures.com)
Human1
  • The retroelement long interspersed element-1 ( LINE-1 or L1) comprises about 17% of the human genome. (researchsquare.com)
Multiple2
  • Individual miRNAs are capable of regulating gene networks by interacting with multiple targets or by suppressing transcriptional controllers [ 6 , 8 ]. (researchsquare.com)
  • Silencing is only disrupted when multiple elements are deleted. (nih.gov)
Target1
  • The zerknullt target gene is repressed by high and low levels of the gradient, so that expression is restricted to the presumptive dorsal ectoderm. (sdbonline.org)
Response elements2
  • It was determined that Ear2 negatively regulates renin expression by competing with retinoic acid receptor/ retinoid X receptor (RAR/RXR) for binding to the retinoic acid response elements (RARE) on the renin enhancer [ 13 ]. (biomedcentral.com)
  • A luciferase assay using a plasmid containing the PXR response elements of CYP3A4 revealed that the increased CYP3A4 induction by siNEAT1_2 or siDAZAP1 was due to the increased transactivation by PXR. (aspetjournals.org)
Short1
  • Notably, the L1 element can retrotranspose itself as well as other retroelements, such as Alu and SVA (SINE-VNTR-Alu: short interspersed element-variable number tandem repeat-Alu). (researchsquare.com)