Reporter gene expression artificial gene fusion. Medical search. Frequent questions

While the observation that NHR-67 forms punctae associated with transcriptional repressors in non-invasive cells is intriguing, the work does not yet established a clear link between the formation and dissolution of NHR-67 condensates with the activation of downstream genes that NHR-67 is actively repressing. (elifesciences.org)
First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. (mdpi.com)
The lacZ reporter gene is downstream of Pctc promoter. (fairdomhub.org)

Randomly generated DNA fragments from bacterial artificial chromosomes containing two clusters of Hox genes were inserted into a vector upstream of a minimal promoter and lacZ reporter gene. (nyu.edu)
lacZ reporter gene is used to monitor sigmaB activity. (fairdomhub.org)

When bait and prey fusion proteins interact in yeast nucleus, the DNA-BD and AD are brought into proximity, thus activating transcription of reporter genes. (bvsalud.org)
This valuable data study presents convincing data that expression of the C. elegans transcription factor NHR-67 is sufficient to drive an invasive fate, and that the alternative proliferative fate is associated with NHR-67 transcriptional down-regulation. (elifesciences.org)
The process of gene expression involves two constitutive steps i.e., transcription and translation and leads to the formation of a protein or polypeptide or in some cases RNA with specific functions. (allindianpatents.com)
The process of transcription is the most important regulatory step in the process of gene expression and its regulation. (allindianpatents.com)
The initiation of transcription and modulation of gene expression in eukaryotic genes is directed by a variety of DNA sequence elements collectively arranged in a larger sequence called promoter. (allindianpatents.com)
The minimal promoter or core promoter is a minimal stretch of contiguous DNA sequence that is sufficient to direct accurate initiation of transcription by RNA [Pol II machinery, Smale, S. T, genes dev 15, 2503-2508 2001]. (allindianpatents.com)
The activation of the core promoter is done by the additional sequence of regulatory DNA sequence elements to which various proteins bind and subsequently interact with the transcription initiation complex to activate gene expression. (allindianpatents.com)
These regulators include canonical genes that control lipid metabolism as well as genes involved in ubiquitination, transcription, and mitochondrial function. (stanford.edu)
Several steps in the gene expression process may be modulated, including the transcription step and the post-translational modification of a protein. (wikidoc.org)
The expression of many genes is regulated after transcription (i.e., by microRNAs or ubiquitin ligases ), so an increase in mRNA concentration need not always increase expression. (wikidoc.org)
Any step of gene expression may be modulated, from the DNA-RNA transcription step to post-translational modification of a protein. (wikidoc.org)
Activated ATF6 mediates transcription of genes encoding ER chaperone proteins also. (cgp60474.com)
NF-κB is a transcription factor that plays a pivotal role in the highly specific pattern of gene expression observed for immune, inflammatory and acute phase response genes, including interleukin 1, interleukin 8, tumor necrosis factor and certain cell adhesion molecules. (justia.com)

The yeast two-hybrid system is a powerful and commonly used genetic tool to investigate the interaction between artificial fusion proteins inside the nucleus of yeast. (bvsalud.org)
Moreover from the analysis of single and multiple mutants we could rule out the involvement of a group of DOF proteins, known as CDFs, already characterised for their involvement in flowering time, in the regulation of AtMYB60 expression. (biomedcentral.com)
For a selected set of transcriptional regulators native proteins are fusion-labelled. (fairdomhub.org)

Next, I produced transgenic mice carrying cre driven by mouse Ins1 using a bacterial artificial chromosome （BAC）construct. (docksci.com)

To investigate the molecular mechanisms governing AtMYB60 expression, its promoter was dissected through deletion and mutagenesis analyses. (biomedcentral.com)
GUS reporter fusions in transgenic plants we were able to demonstrate a modular organization for the AtMYB60 promoter. (biomedcentral.com)
These findings shed light on the regulation of gene expression in guard cells and provide new promoter modules as useful tools for manipulating gene expression in guard cells, both for physiological studies and future biotechnological applications. (biomedcentral.com)
The present invention relates to the designing and synthesis of a completely artificial bidirectional expression module that can also be referred to as bidirectional promoter module. (allindianpatents.com)
The present invention relates to an artificial bidirectional promoter for activation of gene expression in plants. (allindianpatents.com)
Promoter is the portion of DNA sequence on 5' side, i.e. before beginning of the coding region of a gene. (allindianpatents.com)
In this study, I developed cre-reporter and cre-driver mice to facilitate in vivo gene modification, In most reporter mouse strains, it has remained unclear whether a lack of reporter signal indicates either no cre recombinase expression or insufficient reporter gene promoter activity. (docksci.com)

Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. (biomedcentral.com)
The 1.1 Mb critical region contained 6 Axd candidate genes that were then sequenced. (studyres.com)
To determine if a regulatory mutation was responsible for the disease phenotype, the expression levels of the 6 candidate genes were measured. (studyres.com)

This gene thus plays a role in both cell-cycle and epigenetic regulation. (cancerindex.org)
Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation , morphogenesis and the versatility and adaptability of any organism . (wikidoc.org)
Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) the gene in the organism. (wikidoc.org)
Regulation of gene expression is the cellular control of the amount and timing of appearance of the functional product of a gene. (wikidoc.org)
Expression levels of Grhl2 plotted against PNP length reveal a linear relationship indicating the up-regulation of Grhl2 correlates with a failure of PNP closure. (studyres.com)

Here a total of 14 constitutive promoters from S. cerevisiae were cloned and characterized using a green fluorescent protein (GFP) as a reporter in a 2 µ vector pRS426, under varying glucose and oxygen concentrations. (nih.gov)
The bait and prey gene are expressed as a fusion to the GAL4 DNA-binding domain (DNA-BD) and GAL4 activation domain (AD, prey/library fusion protein), respectively. (bvsalud.org)
The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. (cancerindex.org)
What does this gene/protein do? (cancerindex.org)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
Our initial assays with the L. starkeyi secretome expressing differing Tr EGII domains fused to a chimeric Talaromyces emersonii - T. reesei CBHI ( TeTr CBHI) showed that the complete Tr EGII enzyme, including the glycoside hydrolase (GH) 5 domain is required for increased expression level of the fusion protein when linked to CBHI. (biomedcentral.com)
With purified protein and soluble substrates, the exoglucanase activity of the GH7 domain embedded in the Fusion 3 construct in L. starkeyi was significantly higher than that of the GH7 domain in TeTr CBHI expressed alone. (biomedcentral.com)
To facilitate the identification of potential antivirals, we developed two reporter-expressing ZIKVs, each capable of expressing an enhanced green fluorescent protein or an improved luminescent NanoLuc luciferase. (mdpi.com)
The intensity of expression of each reporter protein is measured in the absence of several transcriptional regulators that compete for the RNA polymerase. (fairdomhub.org)
This gene is a member of the protein-tyrosine kinase oncogene family. (cancerindex.org)
What pathways are this gene/protein implicaed in? (cancerindex.org)
The E. coli ompA gene encodes a circular protein that sits in the outer membrane of cells and allows small molecules to enter. (readcodon.com)
The resulting anti recoil was cloned into pETb expression plasmid by fusing 6xHis tag sequence to the N-terminus of the recombinant protein. (red33archi.com)
Choose Gene Evolution libraries for screening projects to improve the properties of your protein of interest. (eurofinsgenomics.eu)
Gene expression is the process by which the inheritable information in a gene , such as the DNA sequence , is made into a functional gene product , such as protein or RNA . (wikidoc.org)
Non-protein coding genes (e.g. rRNA genes, tRNA genes) are not translated into protein. (wikidoc.org)
Another commonly used method for assaying the amount of a particular protein in a cell is to fuse a copy of the protein to a reporter gene such as Green fluorescent protein , which can be directly imaged using a fluorescent microscope. (wikidoc.org)
Because it is very difficult to clone a GFP-fused protein into its native location in the genome, however, this method often cannot be used to measure endogenous regulatory mechanisms (GFP-fusions are therefore most often expressed on extra-genomic DNA such as an expression vector ). (wikidoc.org)
Fusing a target protein to a reporter can also change the protein's behavior, including its cellular localization and expression level. (wikidoc.org)
1000 Mb with up to 18,000 protein-coding genes [ 12 ]. (biomedcentral.com)

The condition is caused by mutations in the transthyretin gene, TTR. (readcodon.com)
USH2A gene mutations in rabbits lead to progressive retinal degeneration and hearing loss. (harvard.edu)
No mutations were found in the coding regions of these genes or the intron-exon boundaries. (studyres.com)

A total of 222 resultant fusion genes were separately electroporated into fertilized eggs, and their regulatory activities were monitored in larvae. (nyu.edu)
Our results have established a regulatory connection between nlt1, ros1 and see1 and suggest the existence of two stages contributing to leaf tumor formation, one before nuclear fusion and involving nlt1 and one after karyogamy that is nlt1 independent. (bvsalud.org)
Additionally, once a transgenic event is obtained, molecular studies required for a commercial release by regulatory authorities, which include transgene insertion site, number of transgenes and gene expression levels, are all hindered by the genomic complexity and the lack of a complete sequenced reference genome for this crop. (frontiersin.org)
Park H, Imoto S , Miyano S. Comprehensive information-based differential gene regulatory networks analysis (CIdrgn): Application to gastric cancer and chemotherapy-responsive gene network identification. (google.com)

100 kb) genomic domains containing Hox genes was performed by using the basal chordate Ciona intestinalis. (nyu.edu)

The study involved reverse genetics, complementation, epistasis analysis, microscopy, gene expression analysis by quantitative reverse transcriptase PCR and virulence assays. (bvsalud.org)
In addition, K-2157 harbored two plasmids: one of 113,644 bp (KPC+) and another of 230,602 bp, containing virulence genes, in addition to an integrative and conjugative element (ICE) embedded on its chromosome, revealing that the presence of these mobile genetic elements mediates the convergence between virulence and antibiotic resistance. (bvsalud.org)

S. cerevisiae has ∼ 6,000 genes, so measuring all pairwise PPIs in one environment requires ∼ 18 million strains to be assayed, each of which characterizes a single PPI. (nature.com)
However, the same improvements were not observed when Fusion 3 construct was expressed in S. cerevisiae and Y. lipolytica . (biomedcentral.com)
Furthermore, S. cerevisiae expression of Fusion 3 construct was poor and only minimal activity was observed when acting on the substrate, p NP-cellobiose. (biomedcentral.com)

Optimise your research and save time with high quality gene synthesis and molecular biology services. (eurofinsgenomics.eu)
Gene synthesis company Eurofins fronting genetic solutions. (eurofinsgenomics.eu)

As an example of application for these promoters in metabolic engineering, the genes involved in xylan degradation and zeaxanthin biosynthesis were subsequently cloned under the control of promoters with medium to high strength and assembled into a single pathway. (nih.gov)
We hypothesise that WIP genes were subsequently co-opted in the control of morphogenesis of novel multicellular structures that evolved during the diversification of land plants. (silverchair.com)

Liu Y, Zhang YZ, Imoto S . Microbial Gene Ontology informed deep neural network for microbe functionality discovery in human diseases. (google.com)

To address this question we first developed human oligonucleotide microarrays with 30.000 elements and then performed large-scale expression profiling of long-term expanded MSC and MSC during differentiation into osteoblasts. (biomedcentral.com)

This reduces the chances of the genes from being silenced by homology based mechanisms. (allindianpatents.com)

Exon 8 of the mouse α 6 subunit gene was disrupted by homologous recombination. (jneurosci.org)

The researchers noticed that, by modifying the ompA 5' sequence and appending it to the start of other genes, they could tune the lifetime of other RNA sequences. (readcodon.com)

Boss, the group except life extension genes, controllable nuclear fusion Apart from the project, where to get VigRX Plus in Kenya other areas are facing strong challenges. (annemarechal.nl)

The pattern of detection of a gene or gene product may be described using terms such as facultative , constitutive , circadian , cyclic , housekeeping , or inducible [1] . (wikidoc.org)

The cre-loxP system is a strategy for controlling temporal and/or spatial gene expression through genome alteration in mice. (docksci.com)
Here, we leverage a set of genome-edited human PLIN2 reporter cell lines in a series of CRISPR-Cas9 loss-of-function screens, identifying genetic modifiers that influence PLIN2 expression and post-translational stability under different metabolic conditions and in different cell types. (stanford.edu)
Recent advances in microarray technology allow for the quantification, on a single array, of transcript levels for every known gene in the human genome. (wikidoc.org)

We tried several gene selection strategies, and built classifiers using the resulting cDNA microarray gene lists. (lu.se)

Tracking human neurologic disease status in mouse brain/plasma using reporter-tagged, EV-associated biomarkers. (harvard.edu)

In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)

The results showed that MSC did not alter their osteogenic differentiation capacity, surface marker profile, and the expression profiles of MSC during expansion. (biomedcentral.com)
Finally we analyzed the gene expression profiles of MSC during osteogenic differentiation. (biomedcentral.com)

In addition to low-throughput methods, transcript levels for many genes at once can be measured with DNA microarray technology or "tag based" technologies like Serial analysis of gene expression (SAGE) or the more advanced version SuperSAGE, which can provide a relative measure of the cellular concentration of different messenger RNAs . (wikidoc.org)

In a series of experiments, the researchers used these dtRNAs to tune the dynamics of genetic circuits, to build paper-based viral diagnostics, and to regulate gene expression in living E. coli cells. (readcodon.com)

To solve this problem, significant expression and secretion levels of heterologous cellulases in yeasts must be achieved. (biomedcentral.com)

The causative gene of the Axd mutation is unknown. (studyres.com)
They were unable to identify a mutation within the critical region, but were able to link the phenotype to an over-expression of Grhl2. (studyres.com)

The 5' untranslated region of this gene forms a secondary structure that changes the stability of RNA sequences. (readcodon.com)

Reporter signals after cre-loxP recombination were detected in pancreatic beta cells of both F 1 mice, but not in other cells. (docksci.com)

To our knowledge, this is the first study in the literature to examine the mRNA expression of CDK2AP1 in human breast cancer over a long-term follow-up period. (cancerindex.org)
In recent years, beta-galactosidase has been researched as a potential treatment for lactose intolerance through gene replacement therapy where it could be placed into the human DNA so individuals can break down lactose on their own. (wikidoc.org)

We found that this new construct ( Tr EGII- TeTr CBHI, Fusion 3) had an increased secretion level of at least threefold in L. starkeyi compared to the expression level of the chimeric TeTr CBHI. (biomedcentral.com)
In Y. lipolytica , TeTr CBHI performed better than the fusion construct. (biomedcentral.com)
It is probable that a higher fraction of fusion construct CBHI is in an active form in Fusion 3 compared to just TeTr CBHI. (biomedcentral.com)

Fertilization consists of a complicated multi-step event: development and maturation of the eggs and sperm, sperm migration into the oviduct, sperm-egg interaction in the oviduct, and then sperm-egg fusion. (docksci.com)

We conclude that the strategy of leading TeTr CBHI expression with a linked Tr EGII module significantly improved the expression of active CBHI in L. starkeyi . (biomedcentral.com)

The Trichoderma reesei eg2 ( Tr EGII) gene was selected as the leading gene due to its previously demonstrated outstanding secretion in yeast. (biomedcentral.com)

They made and characterized more than 80 versions of these dtRNAs, each of which can tune gene expression (via changing RNA stability) to a varying degree. (readcodon.com)

Our objective was to determine if CDK2AP1 mRNA expression levels were consistent with tumour-suppressive functions in breast cancer. (cancerindex.org)
However, neither of these differences in expression levels reached statistical significance. (cancerindex.org)

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In this kind of studies sigmaB stress response is induced by the addition of artificial inducers of sigmaB. (fairdomhub.org)

Although QACs may play an important role in the propagation of antibiotic resistance gene (ARGs), the potential contribution and mechanism remains unclear. (bvsalud.org)

These include eight Hox linked domains that drive expression in nested anterior-posterior domains of ectodermally derived tissues. (nyu.edu)

Anatomy15

Organisms16

Diseases2

Chemicals and Drugs61

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes82

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science3

Health Care1

Downstream3

LacZ2

Transcription13

Proteins3

Bacterial artificial1

Promoter7

Candidate genes3

Regulation5

Protein20

Mutations3

Regulatory4

Genomic1

Virulence2

Cerevisiae3

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Subsequently2

Ontology1

Profiling1

Mechanisms1

Homologous recombination1

Sequence1

Nuclear1

Inducible1

Genome3

CDNA1

Biomarkers1

Vivo1

Differentiation2

Transcript1

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Heterologous1

Mutation2

Sequences1

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Human2

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