• Replicon particles that express bovine viral diarrhea virus sub-genotype 1b E2 glycoprotein were generated and expression was confirmed in vitro using polyclonal and monoclonal antibodies specific to E2. (unl.edu)
  • and (iv) the relative ease of constructing replicon-based vaccines. (asm.org)
  • Replicon particle vaccines administered in a prime/boost regimen expressing BVDV E2 glycoprotein can induce cross-neutralizing titers, reduce leukopenia post challenge, and mitigate clinical disease in calves. (unl.edu)
  • San Diego, CA - Jan. 5, 2017 - Synthetic Genomics, Inc., announced today that it has entered a research and licensing option agreement with Janssen Vaccines & Prevention B.V., part of the Janssen Pharmaceutical Companies of Johnson & Johnson, to apply Synthetic Genomics' proprietary replicon RNA technology toward the design and development of novel RNA-based medicines for specific infectious diseases. (syntheticgenomics.com)
  • On the basis of the pGA1 mini-replicon, an expression vector system was established for C. diphtheriae by means of the P tac promoter and the green fluorescent reporter protein. (springer.com)
  • In addition, other commonly used vector systems from C. glutamicum , including the pBL1 and pHM1519 replicons, and the sacB conditionally lethal selection marker from Bacillus subtilis , were shown to be functional in C. diphtheriae . (springer.com)
  • Importantly, as in human HCV infection, HCV replicon cells neither primed nor boosted a strong NS3/4A-specific T cell response. (bmj.com)
  • We here report that the in vitro anti-norovirus activity of the protease inhibitor rupintrivir is extended to murine noroviruses and that rupintrivir clears human cells from their Norwalk replicon after only two passages of antiviral pressure. (nih.gov)
  • The packaging cell line thus represents a significant advance in the development of the noncytopathic Kunjin virus replicon-based gene expression system and may be widely applicable to the basic studies of flavivirus RNA packaging and virus assembly as well as to the development of gene expression systems based on replicons from different flaviviruses. (asm.org)
  • We previously constructed the first flavivirus replicons based on KUN cDNA by deleting the majority of the genomic region encoding structural genes and retaining only the first 20 codons of the C gene and the last 22 codons of the E gene ( 14 ). (asm.org)
  • The first product of a plasmid DNA replicon is the RNA replicase, which uses the primary positive-strand RNA transcript as a template to make negative-strand RNA and then makes more copies of full-length positive-strand RNA (encoding both replicase and antigen) as well as a shorter subgenome-length mRNA encoding only the antigen ( 17 ). (asm.org)
  • tetKUNCprME cells were also capable of packaging replicon RNA from closely and distantly related flaviviruses, West Nile virus and dengue virus type 2, respectively. (asm.org)
  • The enterovirus protease inhibitor rupintrivir exerts cross-genotypic anti-norovirus activity and clears cells from the norovirus replicon. (nih.gov)
  • Effect of rupintrivir on the clearance of the Norwalk replicon from HG23 cells. (nih.gov)
  • We mimicked this situation in vivo in immune-competent mice by syngeneic transplantation of HCV replicon-containing mouse hepatoma cells. (bmj.com)
  • Design A total of 5 million H-2 b positive Hep56.1D cells, carrying a subgenomic genotype (gt) 2a replicon (HCV replicon cells) or stably expressing comparable levels of the HCV NS3/4A protease/helicase complex (NS3/4A hepatoma cells), were injected subcutaneously into syngeneic H-2 b -restricted mice. (bmj.com)
  • Results HCV replicon and NS3/4A hepatoma cells generated solid tumours in vivo. (bmj.com)
  • Conclusion Syngeneic transplantation of mouse HCV replicon cells into immune-competent animals mirrors many in vivo events in humans. (bmj.com)
  • SH2 modified STAT1 induces HLA-I expression and improves IFN-γ signaling in IFN-α resistant HCV replicon cells. (sigmaaldrich.com)
  • Characterization of these IFN-α resistant replicon cells showed defects in the phosphorylation and nuclear translocation of STAT1 and STAT2 proteins due to a defective Jak-STAT pathway. (sigmaaldrich.com)
  • The activity of the engineered STAT1-CC is dependent upon the phosphorylation of tyrosine residue 701, since the construct with a substituted phenylalanine residue at position 701 (STAT1-CC-Y701F) failed to activate GAS promoter in the replicon cells. (sigmaaldrich.com)
  • The utility of high-titer KUN replicon VLPs was demonstrated by showing increasing CD8 + -T-cell responses to encoded foreign protein with increasing doses of KUN VLPs. (asm.org)
  • Replicon clusters are stable over many cell generations. (rupress.org)
  • Flores thinks viruses may be "the most important faction of the [planet's] biomass" and that a viroid -like entity is a prime candidate for the first replicon on Earth. (huffingtonpost.com)
  • The dotted lines represent 50 and 90% reduction of the relative Norwalk replicon RNA levels. (nih.gov)
  • The enhanced immunogenicity of plasmid DNA replicons cannot be accounted for based only on levels of antigen production but rather through other mechanisms. (asm.org)
  • Replicon is a software company providing timesheet and expense management software. (wikipedia.org)
  • Replicon, Inc. is a software company providing cloud time tracking applications including timesheet and expense management software, using SaaS, for automating employee time tracking, project time tracking, expense tracking and resource scheduling. (wikipedia.org)
  • Withdrawal of tetracycline from the medium resulted in production of Kunjin structural proteins that were capable of packaging transfected and self-amplified Kunjin replicon RNA into the secreted VLPs at titers of up to 1.6 × 10 9 VLPs per ml. (asm.org)
  • All amplified replicons were sequenced by standard procedures and used as specific probes to confirm the replicon typing results by Southern blot hybridization on purified plasmid DNA (data not shown). (cdc.gov)