Replication sequence and bind. Medical search. Frequent questions

Although the structures of rotavirus and other members of the Reoviridae have been extensively studied, little is known about the structures of virus-encoded non-structural proteins that are essential for genome replication and packaging. (nih.gov)
The major enzymatic functions carried out at the replication fork are well conserved from prokaryotes to eukaryotes, but the replication machinery in eukaryotic DNA replication is a much larger complex, coordinating many proteins at the site of replication, forming the replisome. (wikipedia.org)
Multiple replicative proteins assemble on and dissociate from these replicative origins to initiate DNA replication. (wikipedia.org)
Association of the origin recognition complex (ORC) with a replication origin recruits the cell division cycle 6 protein (Cdc6) to form a platform for the loading of the minichromosome maintenance (Mcm 2-7) complex proteins, facilitated by the chromatin licensing and DNA replication factor 1 protein (Cdt1). (wikipedia.org)
The pre-RC formation involves the ordered assembly of many replication factors including the origin recognition complex (ORC), Cdc6 protein, Cdt1 protein, and minichromosome maintenance proteins (Mcm2-7). (wikipedia.org)
Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)
Finally, we demonstrate that Polycomb proteins and associated epigenetic marks are crucial for the control of the replication timing of the INK4a/ARF locus during senescence. (plos.org)
Toward this objective, ChIP-Seq data of 14 S/MAR binding proteins were analyzed and the binding site coordinates of these proteins were used to prepare a non-redundant S/MAR dataset of human genome. (researchgate.net)
Improved yields and quality of templates may be achieved with the use of DNA-binding proteins in amplification and sequencing reactions. (qiagen.com)
This binding puts these ssDNA sequences into optimal conformations for interacting with DNA polymerases and other replication proteins. (qiagen.com)
The smaller replication proteins, Rep40 and Rep52, require ATP to bind DNA, show no sequence specificity in DNA binding and lack the N-terminal nuclease domains. (utoledo.edu)
The communication between molecular motors and double-stranded DNA binding proteins is a common feature in DNA replication, repair, recombination and transcription and also in instances where conflict occurs between these processes. (sciencedaily.com)
As opposed to single strand (ss) DNA binding proteins such as SSB and RPA, which protect and occlude ssDNA from recombination, RAD52/Redβ SSAPs promote recombination and share several biochemical similarities. (nature.com)
They are weak ssDNA binding proteins with no affinity for double-stranded (ds) DNA. (nature.com)
Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. (nih.gov)
In contrast to a chromosomal replication origin, which functionally interacts only with the native DnaA protein of the organism, the ability of RK2 to replicate in a wide range of Gram-negative bacterial hosts requires the interaction of oriV with many different DnaA proteins. (sri.com)
DNase I footprint, gel mobility shift, and surface plasmon resonance analyses showed that the DnaA proteins from Escherichia coli , Pseudomonas putida , and Pseudomonas aeruginosa bind to the DnaA boxes at oriV and are capable of inducing open complex formation, the first step in the replication initiation process. (sri.com)
However, DnaA proteins from two Gram-positive bacteria, Bacillus subtilis and Streptomyces lividans , while capable of specifically interacting with the DnaA box sequences at oriV , do not bind stably and fail to induce open complex formation. (sri.com)
DNA/RNA-binding repeats in PUR-alpha/beta/gamma and in hypothetical proteins from spirochetes and the Bacteroides-Cytophaga-Flexibacter bacteria. (embl-heidelberg.de)
However, a number of HIV proteins have not been successfully inhibited since they lack a reactive site that can bind a small molecule. (sbir.gov)
Performing proof-of-concept studies to demonstrate that small molecule binding to HIV RNA can prevent processing and translation into proteins in relevant cell lines and primary cells. (sbir.gov)
The protein PCNA, which clamps onto DNA, also helps cells resolve replication stress by serving as a platform for repair proteins. (rupress.org)
Researchers have discovered numerous proteins that are involved in replication stress, and Hoffmann et al. (rupress.org)
When the researchers used mass spectrometry to identify the proteins that accumulate at damaged DNA undergoing replication in Xenopus egg extracts, they found one that hadn't turned up in previous studies: the E3 ubiquitin ligase TRAIP. (rupress.org)
This end of TRAIP contains a sequence known as a PIP box that allows proteins to attach to PCNA. (rupress.org)
Some researchers have reported that XNAs containing specific sequences can act as enzymes and bind to proteins. (astrobiology.com)
However, for RNA replication , interactions between nsPs and host proteins are also needed. (bvsalud.org)
CHIKV replication depends on the ability of its replicase proteins to interact with host cell factors, and a better understanding of host cell factor roles in viral infection will increase our understanding of CHIKV RNA replication and provide new strategies for viral infection attenuation. (bvsalud.org)
Here, we demonstrate that the motifs required for the binding of host G3BP/Rin proteins remain functional when transferred from their natural location in nsP3 to different replicase proteins and may enable mutant viruses to complete a full replication cycle. (bvsalud.org)
HMG-box domains are found in one or more copies in HMG-box proteins, which form a large, diverse family involved in the regulation of DNA-dependent processes such as transcription, replication, and strand repair, all of which require the bending and unwinding of chromatin. (embl.de)
AT-hook motifs identified in a wide variety of DNA-binding proteins. (embl.de)
The AT-hook is a small DNA-binding protein motif which was first described in the high mobility group non-histone chromosomal protein HMG-I(Y). Since its discovery, this motif has been observed in other DNA-binding proteins from a wide range of organisms. (embl.de)
We have classified these motifs into three types according to their sequence similarity and have found that they are prevalent in many eukaryotic nuclear proteins in single or multiple copies. (embl.de)
Furthermore, AT-hook motifs are frequently associated with known functional domains seen in chromatin proteins and in DNA-binding proteins (e.g. histone folds, homeodomains and zinc fingers). (embl.de)
It is most interesting that this motif seems to be quite specific to known or predicted chromosomal/DNA-binding proteins, suggesting that it may act as a versatile minor groove tether. (embl.de)
The HMG family of proteins comprises members with multiple HMG domains that bind DNA with low sequence specificity, and members with single HMG domains that recognize specific nucleotide sequences. (embl.de)
There is no evidence that the SIR proteins are sequence-specific DNA-binding proteins. (utoronto.ca)

The non-structural protein NSP2 of rotavirus, which exhibits nucleoside triphosphatase, single-stranded RNA binding, and nucleic-acid helix-destabilizing activities, is a major component of viral replicase complexes. (nih.gov)
Eukaryotic origins of replication control the formation of several protein complexes that lead to the assembly of two bidirectional DNA replication forks. (wikipedia.org)
Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
Moreover, we show that the Polycomb protein BMI1 interacts with CDC6, an essential regulator of DNA replication in eukaryotic cells. (plos.org)
Upon senescence, Jmjd3 is overexpressed and the MLL1 protein is recruited to the locus provoking the dissociation of Polycomb from the INK4/ARF locus, its transcriptional activation and its replication during early S-phase. (plos.org)
The native Gene 32 Protein from bacteriophage T4 (T4gp32) is a single-stranded DNA binding protein that is required for T4 DNA replication, recombination and repair. (qiagen.com)
The T4 Gene 32 Protein has exhibited an ability to enhance the performance of several DNA synthesis-related activities in secondary-structure rich regions, including PCR amplification and DNA sequencing. (qiagen.com)
The T4 Gene 32 Protein is a single-stranded nucleic acid binding protein that has the function of stabilizing single-stranded regions of DNA. (qiagen.com)
The ability of T4 Gene 32 Protein to enhance the performance of several DNA synthesis-related activities is based on its essential function in the replication of bacteriophage T4. (qiagen.com)
DNA binding of single stranded DNA by T4 Gene 32 Protein was measured using a gel shift assay with a single-stranded, fluorescently labeled oligonucleotide. (qiagen.com)
Studies of the thermodynamics of binding of ligands to glycyl tRNAs suggest that the formation of glycyl adenylate, an obligate intermediate on the reaction pathway, is accompanied by a significant conformation change in the protein that alters the affinity of the enzyme for tRNA. (utoledo.edu)
A termination site comprises a 23-base pair termination sequence (Ter) bound to the protein terminus utilization substance (Tus). (sciencedaily.com)
Also, they share a similar protein architecture based on an N-terminal ssDNA binding domain of ~180 amino acids and a C-terminal extension that in the case of RAD52 is required for homologous recombination (HR) through specific protein-protein interactions 6 . (nature.com)
These shared biochemical, protein sequence and functional similarities suggest the existence of an ancestral annealing mechanism involved in HR. (nature.com)
The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. (nih.gov)
Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. (nih.gov)
The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. (nih.gov)
Replication initiation of the broad host range plasmid RK2 requires binding of the host-encoded DnaA protein to specific sequences (DnaA boxes) at its replication origin ( oriV ). (sri.com)
The purine-rich element binding (Pur) protein family protein consists PURalpha/beta/gamma in humans. (embl-heidelberg.de)
Pur-alpha is a highly conserved, sequence-specific DNA- and RNA-binding protein involved in diverse cellular and viral functions including transcription, replication, and cell growth. (embl-heidelberg.de)
Association of human Pur alpha with the retinoblastoma protein, Rb,regulates binding to the single-stranded DNA Pur alpha recognitionelement. (embl-heidelberg.de)
The retinoblastoma protein, Rb, is detected in extracts of monkey CV-1cells complexed with Pur alpha, a sequence-specific single-strandedDNA-binding protein implicated in control of gene transcription and DNAreplication. (embl-heidelberg.de)
Human Pur alpha, expressed as a glutathioneS-transferase fusion protein, specifically binds to the hypophosphorylatedform of Rb with an affinity as high as that of SV40 large T-antigen. (embl-heidelberg.de)
Inthe absence of DNA, glutathione S-transferase-Pur alpha binds to p56RB, anNH2-terminal-truncated Rb protein purified from Escherichia coli,containing the T-antigen binding domain, to form multimeric complexes. (embl-heidelberg.de)
Sequence of cDNA comprising the human pur gene and sequence-specificsingle-stranded-DNA-binding properties of the encoded protein. (embl-heidelberg.de)
We report here the cloning and sequencing of a cDNA encodinga protein with strong affinity for the PUR element. (embl-heidelberg.de)
We report that a localized inducible protein tether between the chromosome and cell membrane in E. coli cells imparts a rapid and complete block to replication initiation. (nih.gov)
Tethers, composed of a trans-membrane and transcription repressor fusion protein bound to an array of operator sequences, can be placed up to 1 Mb from the origin with no loss of penetrance. (nih.gov)
Sequence-specific protein recognition of single-stranded nucleic acids is critical for many fundamental cellular processes, such as DNA replication, DNA repair, transcription, translation, recombination, apoptosis and telomere maintenance. (rcsb.org)
To explore the mechanisms of sequence-specific ssDNA recognition, we determined the crystal structures of 10 different non-cognate ssDNAs complexed with the Oxytricha nova telomere end-binding protein (OnTEBP) and evaluated their corresponding binding affinities (PDB ID codes 1PH1-1PH9 and 1PHJ). (rcsb.org)
As a consequence of inhibition of a target enzyme protein, namely, RNA-dependent RNA polymerase there is a probability of the prevention of virus replication in the host cells. (academicjournals.org)
Many viruses use the microtubule transport system to transport nucleic acid/protein cores to intracellular replication sites after invasion host the cell membrane. (wikipedia.org)
By developing small molecules to selectively bind to key sites on transcribed HIV RNA the translation of RNA to protein may be inhibited for any HIV intracellular protein. (sbir.gov)
Project Goal The goal of this SBIR solicitation is to support the discovery and design of RNA-targeted small molecules which specifically bind to HIV RNA transcripts to prevent RNA processing and translation into protein. (sbir.gov)
PCBP1 is a single-stranded nucleic acid binding protein that binds preferentially to oligo dC. (prospecbio.com)
PCBP1 along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. (prospecbio.com)
High mobility group (HMG) box domains are involved in binding DNA, and may be involved in protein-protein interactions as well. (embl.de)
Using pattern searches and position-dependent matrices, we have extracted the AT-hook motifs present in a non-redundant protein sequence database. (embl.de)
In general, it appears that the AT-hook motif is an auxiliary protein motif cooperating with other DNA-binding activities and facilitating changes in the structure of the DNA either as a polypeptide on its own [e.g. (embl.de)
There is no evidence that this is a sequence-specific DNA-binding protein, either in vitro or in vivo or in its sequence. (utoronto.ca)
No direct evidence that this is a DNA-binding protein. (utoronto.ca)
Gcr2 is not a DNA-binding protein. (utoronto.ca)
Swi6 is a cofactor, not a DNA-binding protein. (utoronto.ca)
Of greatest interest is the~300 amino acid receptor binding domain (RBD) within the S1 subunit of the spike protein because of its key interaction with the human angiotensin converting enzyme 2 (hACE2) receptor present on many cell types, especially lung epithelial cells. (cdc.gov)
By means of DNA sequencing of a large sample collection of the pathogen from across the United States, we studied the gene for the bacterium's highly diverse OspC protein, protective immunity against which develops in animals. (cdc.gov)
the c-terminal membrane-binding portion contributes to the Golgi disassembly and, therefore, functionally similar to the picornavirus 2B protein" /protein_id="YP_009238492.1" mat_peptide 995. (cdc.gov)
the calicivirus NTPase was found in membranous replication complexes" /protein_id="YP_009238487.1" mat_peptide 2093. (cdc.gov)
the FCV ortholog was detected in membranous replication complexes" /protein_id="YP_009238488.1" mat_peptide 2630. (cdc.gov)
This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA. (lu.se)
Nitrate, thiocyanate, and perchlorate can reversibly bind to the sodium-iodide symporter (NIS) protein resulting in reduced iodine absorption by the thyroid. (cdc.gov)

They used single-molecule imaging to record molecular movies that zoomed in with high temporal and spatial resolution on the fate of Escherichia coli replication forks as they approached a termination site from either direction. (sciencedaily.com)
The recent Encyclopedia of studies in the 1950s and 1960s in Escherichia coli and then by DNA Elements (ENCODE) project showed binding of carcinogens to nu- Szybalski (1958) , who tested more indicates that at least 80% of the cleic acids ( Wiest and Heidelberger, than 400 compounds in E. coli . (who.int)

A new U.S. study published on the bioRxiv * preprint server reports the use of an amiloride-based scaffold that targets a viral RNA structure known to be essential in viral replication in the 5'-untranslated region (UTR) of the virus. (news-medical.net)
The virus exploits the host machinery to facilitate efficient viral replication, which ultimately leads to progression of infection. (news-medical.net)
These are apparently cis-elements involved in regulating viral replication. (news-medical.net)
Dynein and kinesin can both be exploited by viruses to mediate the viral replication process. (wikipedia.org)
Targeting one or more HIV RNA sequences with small molecules may be an effective way of shutting down viral replication, preventing cellular transmission and ultimately leading to sustained viral remission. (sbir.gov)
To date, host innate immunity may be the only identified direct factor associated with viral replication. (spandidos-publications.com)

Using in vitro binding studies, the researchers verified that TRAIP's C-terminal domain binds to PCNA through the PIP box. (rupress.org)
Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
it contains no DNA-binding domain and has no known in vitro sequence specificity. (utoronto.ca)

Triplet substrates thus resolve a central incongruity of RNA replication, and here allow the ribozyme to synthesise its own catalytic subunit '+' and '-' strands in segments and assemble them into a new active ribozyme. (elifesciences.org)

The priming event on the lagging strand establishes a replication fork. (wikipedia.org)
An artist in its own right, Ligase dutifully connects Okazaki fragments (short sequences produced at the lagging strand) during replication. (aboutdarwin.com)
By coating these transiently exposed ssDNA sequences, gp32 also protects them from degradation by nucleases while they discharge their templating (and other) functions in association with the leading- and lagging-strand DNA polymerases. (qiagen.com)

The controlled assembly of replication forks is critical for genome stability. (imperial.ac.uk)
To ensure the process is well regulated, the bacterium has set a number of "roadblocks," or termination sites on the DNA, to ensure the permanent stoppage of replication forks, Y-shaped structures formed between the strands as the DNA molecule splits. (sciencedaily.com)
Tus−Ter is unusual in that it acts like the ratcheting knot on a climbing rope by allowing progression of replication forks from one direction but not the other. (sciencedaily.com)
Tether-induced initiation blocking has no effect on elongation at pre-existing replication forks and does not cause cell or DNA damage. (nih.gov)
Replication stress refers to the various impediments that can slow or stall replication forks. (rupress.org)
Because stalled forks can lead to DNA double-strand breaks and genome instability, cells take a series of measures to clear the obstacles and restart replication ( 2 ). (rupress.org)

The R3H motif: a domain that binds single-stranded nucleic acids. (embl-heidelberg.de)

The identification of detailed RNA structures now allows the design of small molecules which are capable of binding to RNA with high selectivity and specificity. (sbir.gov)
These surfaces are defined empirically based on failure to identify small, drug-like molecules that bind to them with high affinity and specificity. (stanford.edu)
no DNA-binding domain and no evidence for direct interaction with DNA or intrinsic sequence specificity. (utoronto.ca)

The human Pur factor binds strongly to a sequence element repeated withinzones of initiation of DNA replication in several eukaryotic cells. (embl-heidelberg.de)

Eukaryotic DNA replication of chromosomal DNA is central for the duplication of a cell and is necessary for the maintenance of the eukaryotic genome. (wikipedia.org)
In order to isolate a particular gene from human chromosomal DNA, it would be necessary to isolate a sequence of a few hundred or few thousand basepairs from the entire human genome. (addgene.org)

In analyzing the stability of DNA replication origins in Saccharomyces cerevisiae we faced the question whether one set of sequences is significantly enriched in the number and/or the quality of the matches of a particular position weight matrix relative to another set. (biomedcentral.com)
In analyzing the stability of DNA replication origins in S. cerevisiae (see Stable vs. unstable ARSs in mcm1-1 mutant below) we faced the question of whether one set of sequences has more and/or better binding sites of a particular transcription factor than the other. (biomedcentral.com)
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C . (yeastgenome.org)
The S. cerevisiae ORC binds to specific DNA sequences throughout the cell cycle but becomes active only when it binds to the replication initiator Cdc6. (imperial.ac.uk)

Both crystal structures revealed that the N-terminal domain of ~200 amino acids forms a mushroom-shaped undecameric ring with an external groove lined with positive charges, which probably binds the phosphodiester backbone of ssDNA. (nature.com)

We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)

Complete DNA sequences can be used as a platform to develop rapid epidemiologic tools to identify and trace the spread of plasmids in clinically relevant pathogens, thus facilitating a better understanding of their distribution and ability to transfer between bacteria of humans and animals. (cdc.gov)
In most bacteria, chromosome segregation is achieved by the ParABS system, in which the ParB-bound parS sequence is actively partitioned by ParA. (jic.ac.uk)
Bdellovibrio bacteriovorus is a predatory bacterium proliferating through polyploid replication and non-binary division inside other bacteria. (jic.ac.uk)

Here we analyse five whole genome sequences of V. coralliilyticus to examine whether virulence is similarly driven by horizontally acquired elements. (nature.com)

In summary, our work provides fundamental insights into DDK structure, control and selective activation of the MCM2-7 helicase during DNA replication. (imperial.ac.uk)
Gp32 binds transiently and cooperatively to ssDNA template sequences as these entities are exposed by the processive helicase operating within the replication complex. (qiagen.com)

There has also been work on discriminative de-novo motif finding (e.g., [ 10 ]) where the goal is to find a PWM that discriminates between two sets of sequences. (biomedcentral.com)
The researchers aim to exploit the conservation of certain SARS-CoV-2 RNA motif sequences and structures. (news-medical.net)
Binding of Pur alpha top56RB, the COOH-terminal portion of Rb, is inhibited by a syntheticpeptide containing the T-antigen Rb-binding motif. (embl-heidelberg.de)
The high-mobility group (HMG) domain is a DNA-binding motif that is shared abundant non-histone components of chromatin and by specific regulators of transcription and cell differentiation. (embl.de)
I do not see how either of these motifs could possibly be a Gal4-class binding motif. (utoronto.ca)

Concurrently, the sequence register of the ssDNA shifts to re-establish a more cognate-like pattern. (rcsb.org)

Theprotein binds preferentially to the purine-rich single strand of thiselement, PUR. (embl-heidelberg.de)

Although it has been recognized for several decades that chromosome structure regulates the capacity of replication origins to initiate, very little is known about how or if cells actively regulate structure to direct initiation. (nih.gov)

DNA region that is required for the binding of chromatin to the nuclear matrix. (insdc.org)

Using the right sequences, they can induce such particles to assemble themselves into complex objects. (newscientist.com)
used siRNA to deplete TRAIP in cells exposed to agents that induce replication stress. (rupress.org)

To synthesize DNA, the double-stranded DNA is unwound by DNA helicases ahead of polymerases, forming a replication fork containing two single-stranded templates. (wikipedia.org)
The process of semiconservative replication for the site of DNA replication is a fork-like DNA structure, the replication fork, where the DNA helix is open, or unwound, exposing unpaired DNA nucleotides for recognition and base pairing for the incorporation of free nucleotides into double-stranded DNA. (wikipedia.org)
This enzyme unwinds the double-stranded DNA, paving the way for replication. (aboutdarwin.com)

Subsequently, the complete genomic sequences of five PCV4 strains were obtained, of which one PCV4 strain (SX-ZX) was from Shaanxi province, and these strains were 1770 nucleotides in length and had 97.7%-99.4% genomic identity with 59 PCV4 reference strains. (bvsalud.org)

Insertion of native motifs into nsP1 or nsP2 but not into the C terminus of nsP4 activated CHIKV RNA replication in human cells in a G3BP-dependent manner. (bvsalud.org)

DNA sequences containing these sites were initially isolated in the late 1970s on the basis of their ability to support replication of plasmids, hence the designation of autonomously replicating sequences (ARS). (wikipedia.org)
In this study, we report the full sequence and analysis of pCT and demonstrate the spread of pCT-like plasmids in animal and human E. coli isolates from the United Kingdom, Europe, Australia, and Asia. (cdc.gov)
Typically plasmids contain the minimum essential DNA sequences for this purpose, which includes a DNA replication origin, an antibiotic-resistance gene, and a region in which exogenous DNA fragments can be inserted. (addgene.org)
Direct Colony Sequencing is also extremely effective when low amounts of DNA starting material are available (e.g. low copy plasmids). (genewiz.com)

Here we report the cryo-EM structure at 3.3 Å resolution of the yeast ORC-Cdc6 bound to an 85-bp ARS1 origin DNA. (imperial.ac.uk)
Cdc6 binding rearranges a short α-helix in the Orc1 AAA+ domain and the Orc2 WHD, leading to the activation of the Cdc6 ATPase and the formation of the three sites for the recruitment of Mcm2-7, none of which are present in ORC alone. (imperial.ac.uk)
We identified the replication licencing factor CDC6 as a new partner of the Polycomb group member BMI1. (plos.org)

Experiments on colloids have proved that self-replication is possible, they say. (newscientist.com)

with the formation of the pre-replication complex (pre-RC) being a key intermediate in the replication initiation process. (wikipedia.org)

In G1 phase of the cell cycle, many of the DNA replication regulatory processes are initiated. (wikipedia.org)
Here we show that in young proliferating embryonic fibroblasts (MEFs) the Polycomb Repressive Complex 2 (PRC2) member EZH2 together with PRC1 members BMI1 and M33 are strongly expressed and localized at the INK4/ARF regulatory domain (RD) identified as a DNA replication origin. (plos.org)

DNA replication is the action of DNA polymerases synthesizing a DNA strand complementary to the original template strand. (wikipedia.org)
This transition involves the ordered assembly of additional replication factors to unwind the DNA and accumulate the multiple eukaryotic DNA polymerases around the unwound DNA. (wikipedia.org)

Studies on the GM process have shown that the foreign gene inserts invariably damages the genome, scrambling and rearranging DNA sequences, resulting in inappropriate gene expression that can trigger cancer [3, 4]. (i-sis.org.uk)
Gene expression patterns, assayed by RNA sequencing, show that tethering induces global supercoiling changes, which are likely incompatible with replication initiation. (nih.gov)
Thanks to molecular biology techniques like DNA sequencing and gene expression analysis, biologists have made significant strides in understanding genetics and genomics. (freescience.info)

Interesting, because the molecule contains 2 consensus sequences for ATP-binding (Walker-sequences), so either binding occurs also to non-Walker sequences or (more likely) there is co-operation between molecules. (bio.net)
region on a DNA molecule involved in RNA polymerase binding to initiate transcription. (insdc.org)
Examples DNA repair and apoptosis path- concept is that there is a distinct dif- of DNA damage are DNA adducts ways, which are described in detail ference between DNA damage and (i.e. a molecule bound covalently to by Ciccia and El edge (2010) . (who.int)

However, as similar as they may appear to those newly introduced to these concepts, replication and transcription have unique roles to play in cellular activity. (aboutdarwin.com)

The results illuminate the molecular mechanism of a critical biochemical step in the licensing of eukaryotic replication origins. (imperial.ac.uk)
DNA binding ability was observed as a band shift in the apparent molecular weight of the oligonucleotide on the TBE-Urea gel. (qiagen.com)
To explore the concepts of genomics, molecular biologists employ cutting-edge techniques such as PCR (polymerase chain reaction), cloning, sequencing, and genetic engineering. (freescience.info)
Molecular biology techniques, such as DNA sequencing and genotyping, have facilitated the identification of genetic variations that impact drug metabolism and efficacy. (freescience.info)

A cell may run short of nucleotides to plug into the new DNA strands, for example, or the replication machinery might run into a section of DNA that is damaged or tricky to copy. (rupress.org)
They also made a longer L-aTNA with a nucleobase sequence that complemented the sequences of the fragments, similar to how DNA strands match up. (astrobiology.com)
Using three-letter 'triplet' building blocks, this new ribozyme can copy various folded RNA strands, including the active part of its own sequence. (elifesciences.org)
For example, these triplets work together to bind tightly to RNA strands and unravel structures that block RNA copying. (elifesciences.org)
2001). The synthesized strands are displaced after one round of RCA and are free for additional rounds of primer binding. (genewiz.com)

Architecture of the yeast origin recognition complex bound to origins of DNA replication. (harvard.edu)

In early January 2020, the specific virus was isolated and later identified as a novel coronavirus by sequencing ( 3 ). (spandidos-publications.com)
RÉSUMÉ Une analyse documentaire des informations publiques disponibles a été entreprise afin de passer en revue les connaissances et les lacunes actuelles sur le coronavirus du syndrome respiratoire du Moyen-Orient (MERS-CoV), notamment sur son origine, la transmission, les mesures de lutte efficaces et la prise en charge. (who.int)

When cells enter senescence the binding to RD of both PRC1 and PRC2 complexes is lost leading to a decreased level of histone H3K27 trimethylation (H3K27me3). (plos.org)
The chromo-domain binds histone H3 tails methylated on lysine 9. (cipsm.de)

Eukaryotic DNA replication is a conserved mechanism that restricts DNA replication to once per cell cycle. (wikipedia.org)
This mechanism is conserved from prokaryotes to eukaryotes and is known as semiconservative DNA replication. (wikipedia.org)
RCA was identified in natural systems as a DNA replication mechanism frequently employed in plasmid and viral propagation. (genewiz.com)

Click "Sequence Details" to view all sequence information for this locus, including that for other strains. (yeastgenome.org)
To further clarify the origin and prevalence of PCV4, a total of 152 clinical samples collected from 49 different swine farms of 15 cities in Henan Province of China from 2011 to 2021 were tested for the presence of PCV4 by qPCR, and the complete genome of PCV4 strains was amplified from the positive samples and sequenced. (bvsalud.org)
In addition, we sequenced 99 ospC flanking sequences from different lineages and compared the complete cp26 sequences of 11 strains as well as the cp26 bbb02 loci of 56 samples. (cdc.gov)

The Origin Recognition Complex (ORC) binds to sites in chromosomes to specify the location of origins of DNA replication. (imperial.ac.uk)

It has a small (4500 nucleotides) genome that encoding four DNA helicases that are required for viral DNA replication and efficient packaging of single stranded DNA into virions. (utoledo.edu)
A mutation is defined as a sing of the DNA damage by the cel by replicating past the unrepaired change in the sequence or number (the DNA damage response), and DNA damage, thereby introducing a of nucleotides in the DNA. (who.int)

DNA replication is a fundamental process essential for cell proliferation. (biomedcentral.com)

We've identified a new factor that has an important role in the response to replication stress," says Mailand. (rupress.org)

Azenta Life Sciences, formerly GENEWIZ's Direct Colony Sequencing services utilizes rolling circle amplification (RCA) to enable the Sanger sequencing of bacterial clone or phage sample templates without the need for plasmid preparation. (genewiz.com)
Using RCA for DNA amplification expedites the Sanger sequencing process and allows your data to be delivered within 24 hours** of sample receipt at Azenta. (genewiz.com)
Indeed, if the sequence of interest is present in the DNA extract, it is possible to selectively replicate it (we speak of amplification) in very large numbers. (intechopen.com)

The virus enters human cells via endocytosis by binding the ACE2 receptor and releasing its positive-sense RNA genome. (news-medical.net)

Rep78 and Rep68 also have a site specific nuclease activity, residing in an N-terminal structure, that creates a priming site in the template for DNA replication. (utoledo.edu)
Homology models of RNA-dependent RNA polymerase domain was finally taken into consideration to focus on the prediction of the specific ligand binding site by docking against antiviral drug Ribavirin, with an aid of AutoDock4.2. (academicjournals.org)
[11] Not much is known about virus' motor-specific binding sites, but it is known that some viruses contain proline-rich sequences (that diverge between viruses) which, when removed, reduces dynactin binding, axon transport (in culture), and neuroinvasion in vivo. (wikipedia.org)
As the programs for the simulations such as Amber, CHARMM, BOSS and so on have good parameters for DNA, they succeeded in producing dynamic features of complexes of DNA and fluorescent materials (FMs).However, it is necessary to always make parameters for each FM when new FMs are designed for a specific DNA sequence. (int-conf-chem-structures.org)
Digesting the human genome with restriction enzymes would yield about two million DNA fragments, which is far too many to separate from each other for the purposes of isolating one specific DNA sequence. (addgene.org)
PCBP1 plays a role in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. (prospecbio.com)
I found no evidence that it is a sequence-specific TF. (utoronto.ca)
No evidence for sequence-specific DNA-binding. (utoronto.ca)
No evidence this is a sequence-specific TF. (utoronto.ca)

The reference sequence is identical to AB044366. (cdc.gov)

In the present study, we adopted MO calculations to investigate interactions between FMs and eight DNA hexamers with sequences such as AAAAAA, TTTTTT, AAATTT, TTTAAA, ATATAT, TATATA, GGGGGG and CCCCCC. (int-conf-chem-structures.org)
The hexamers anneal to the circular template, and replication initiates at many sites simultaneously. (genewiz.com)
As essential elements for the replication, the 17-bp iterative sequence was predicted as the stem structure, in which three non-tandem hexamers were found at downstream with H1/H2 (12-CGGCACACTTCGGCAC-27) as the minimal binding site. (bvsalud.org)

Azenta RCA protocols can handle sequencing projects of any size, and are optimized to produce high quality reads from both bacterial colonies and glycerol stocks as well as phage plaques and supernatants. (genewiz.com)
DNA methylation analysis: Save time & obtain high-quality reads by cloning PCR products directly and sequencing the bacterial colonies. (genewiz.com)

None of these however directly apply to our problem, where the null assumption is that there is "essentially" no difference in the binding sites between the two input sets, even though both might be enriched, deficient, or neutral in sites when compared to "background" sequences. (biomedcentral.com)
Several RNA binding sites, resulting from the quaternary organization of NSP2 monomers, may be required for the helix destabilizing activity of NSP2 and its function during genome replication and packaging. (nih.gov)
Mdr1 (and presumably other ABC-type ATPases) has no co-operation between ATP-binding sites? (bio.net)
It binds Swi6 and the ChIP motifs all resemble Swi4 binding sites. (utoronto.ca)
3028 /gene="ORF1" /locus_tag="NoVGII_gp1" /product="VPg" /note="For Southampton calicivirus, both N-terminal and C-terminal cleavage sites have been confirmed by direct sequencing. (cdc.gov)

Initiation of eukaryotic DNA replication is the first stage of DNA synthesis where the DNA double helix is unwound and an initial priming event by DNA polymerase α occurs on the leading strand. (wikipedia.org)
The triplets cooperatively invaded and unraveled even highly stable RNA secondary structures, and support non-canonical primer-free and bidirectional modes of RNA synthesis and replication. (elifesciences.org)

G3BP/Rin-Binding Motifs Inserted into Flexible Regions of nsP2 Support RNA Replication of Chikungunya Virus. (bvsalud.org)
In this study, we inactivated G3BP/Rin-binding motifs in the HVD and inserted peptides containing either native or inactivated G3BP/Rin-binding motifs into flexible regions of nsP1, nsP2, or nsP4. (bvsalud.org)
Thus, the binding of G3BPs to nsP2 can support CHIKV RNA replication and restore the infectivity of viruses lacking G3BP-binding motifs in the HVD of nsP3. (bvsalud.org)

Anatomy22

Organisms41

Diseases2

Chemicals and Drugs90

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes85

Information Science3

Health Care2

Proteins37

Protein51

Escherichia2

Viral replication6

Vitro3

Subunit1

Lagging strand3

Forks6

Single-stranded nucleic acids1

Specificity3

Eukaryotic cells1

Chromosomal2

Cerevisiae4

Probably binds1

Nucleotide sequences1

Bacteria3

Whole genome1

Helicase2

Motif5

SsDNA1

Preferentially1

Origins to initiate1

Chromatin1

Induce2

Double-stranded3

Genomic1

Terminus1

Plasmids4

Cdc63

Self-replication1

Initiation process1

Regulatory2

Polymerases2

Gene expression3

Molecule3

Cellular1

Molecular4

Strands5

Yeast1

Coronavirus2

Histone2

Mechanism3

Strains3

Chromosomes1

Nucleotides2

Cell proliferation1

Factor1

Amplification3

ACE21

Specific9

Reference sequence1

Hexamers3

Bacterial2

Sites5

Synthesis2

NSP23