Replication origin. Medical search. Frequent questions

The origin recognition complex (ORC) is essential for initiation of eukaryotic chromosome replication as it loads the replicative helicase-the minichromosome maintenance (MCM) complex-at replication origins 1 . (nature.com)
Our results establish that ORC, in addition to its canonical role as the MCM loader, has a second crucial function as a master regulator of nucleosome organization at the replication origin, a crucial prerequisite for efficient chromosome replication. (nature.com)
Chromosome replication in bacteria is regulated at the initiation stage. (wikipedia.org)
Among these, Vibrio cholerae , with two chromosomes has served as a model system to study various aspects of chromosome replication, maintenance and faithful partitioning of multipartite genomes. (exordo.com)
The implications of our findings with respect to mechanistic aspects of chromosome replication, partitioning and maintenance of single chromosome with multiple origins and the biological, functional and evolutionary significance of multipartite genome architecture in general will be discussed. (exordo.com)
Long gaps between active replication origins probably occur frequently during chromosome replication, but little is known about how cells cope with them. (nyu.edu)

Propagation of the genetic material between generations requires timely and accurate duplication of DNA by semiconservative replication prior to cell division to ensure each daughter cell receives the full complement of chromosomes. (wikipedia.org)
OGRE density along chromosomes correlated with previously published replication timing data. (crcm-marseille.fr)
Telomeres and DNA Replication Telomeres: Theendsof eukaryoticchromosomes (chromosomes arelinear) Neededfor chromosomal integrity andstability. (slideshare.net)
We have previously shown that replication of fission yeast chromosomes is initiated in distinct regions. (elsevierpure.com)
To address this issue, we deleted replication origins from S. cerevisiae chromosome III to create chromosomes with long interorigin gaps and identified mutations that destabilize them [originless fragment maintenance (Ofm) mutations]. (nyu.edu)
Deletion of the H3K56 acetylase and downstream chromatin assembly factors suppressed the Ofm phenotype of hst3, indicating that persistence of H3K56Ac-containing chromatin is deleterious for the maintenance of ORIΔ chromosomes, and experiments with synchronous cultures showed that it is replication of H3K56Ac-containing chromatin that causes chromosome loss. (nyu.edu)

Nonetheless, research subsequent to Jacob's, Brenner's and Cuzin's proposal of the replicon model has discovered many additional layers of replication control in bacteria and eukaryotes that comprise both positive and negative regulatory elements, highlighting both the complexity and the importance of restricting DNA replication temporally and spatially. (wikipedia.org)
The genetic endowment of most bacteria is encoded in a single chromosome and is inherited by the daughter cells through bi directional replication of the parental DNA from a single origin of replication. (exordo.com)
However, some bacteria have more than one chromosome with independent origins of replication in each chromosome. (exordo.com)

Once bound to replicators, initiators (often with the help of co-loader proteins) deposit replicative helicases onto DNA, which subsequently drive the recruitment of additional replisome components and the assembly of the entire replication machinery. (wikipedia.org)
In contrast to a chromosomal replication origin, which functionally interacts only with the native DnaA protein of the organism, the ability of RK2 to replicate in a wide range of Gram-negative bacterial hosts requires the interaction of oriV with many different DnaA proteins. (sri.com)
DNase I footprint, gel mobility shift, and surface plasmon resonance analyses showed that the DnaA proteins from Escherichia coli , Pseudomonas putida , and Pseudomonas aeruginosa bind to the DnaA boxes at oriV and are capable of inducing open complex formation, the first step in the replication initiation process. (sri.com)
To investigate the dynamic changes in chromatin organization at and surrounding replication origins, we used micrococcal nuclease (MNase) to generate genome-wide chromatin occupancy profiles of nucleosomes, transcription factors, and replication proteins through consecutive cell cycles in Saccharomyces cerevisiae. (duke.edu)
Random fluctuations in the formation of replication starting points, called origins, and the subsequent activation of proteins lead to variations in the replication time. (elsevierpure.com)
Here, we present evidence that fission yeast replication origins are specifically associated with proteins that participate in initiation of replication. (elsevierpure.com)
The results suggest that the fission yeast ORC specifically interacts with chromosomal replication origins and that Mcm proteins are loaded onto the origins to play a role in initiation of replication. (elsevierpure.com)
We have shown that E1 and E2 proteins of human papillomavirus type 11 (HPV-11) were essential to support the replication of the homologous viral origin (ori) in a transient replication assay, similar to reports on bovine papillomavirus type 1 (BPV-1). (elsevierpure.com)
Either set of viral proteins was also able to initiate replication of ori-containing plasmids from many other human and animal papillomaviruses. (elsevierpure.com)
Recent breakthroughs have uncovered more and more DNA replication licensing machinery proteins (ORC, Cdc6, Cdt1, geminin, etc.) functioning in other cell cycle events, including centrosome replication, mitotic events, transcription and so on. (intechopen.com)
DNA replication origins retain mobile licensing proteins. (yeastgenome.org)
Most cellular proteins involved in genome replication are conserved in all eukaryotic lineages including yeast, plants and animals. (bvsalud.org)

In the elongation phase, replisomes travel in opposite directions with the replication forks, unwinding the DNA helix and synthesizing complementary daughter DNA strands using both parental strands as templates. (wikipedia.org)
After origin firing, bidirectional replication forks travel until they reach termination sites. (biomedcentral.com)
Tether-induced initiation blocking has no effect on elongation at pre-existing replication forks and does not cause cell or DNA damage. (nih.gov)
Finally, we observed nucleosome disruption and disorganization emanating from replication origins and traveling with the elongating replication forks across the genome in S phase, likely reflecting the disassembly and assembly of chromatin ahead of and behind the replication fork, respectively. (duke.edu)
H3K56Ac-containing nucleosomes are preferentially assembled into chromatin behind replication forks. (nyu.edu)

Structural changes in Mcm5 protein bypass Cdc7-Dbf4 function and reduce replication origin efficiency in Saccharomyces cerevisiae. (yeastgenome.org)

Both Orp1p and SpMcm6p were associated with a 1-kb region that contains elements required for autonomous replication of ars2004. (elsevierpure.com)
According to current evidence, several elements are required for autonomous λ DNA replication. (cshmonographs.org)

The putative origins identified in T. cruzi show a notable enrichment of GC content, a preferential position at subtelomeric regions, coinciding with genes transcribed towards the telomeres, and a pronounced enrichment within coding DNA sequences, most notably in genes from the Dispersed Gene Family 1 (DGF-1). (biomedcentral.com)
These findings suggest a scenario where collisions between DNA replication and transcription are frequent, leading to increased genetic variability, as seen by the increase SNP levels at chromosome subtelomeres and in DGF-1 genes containing putative origins. (biomedcentral.com)
Most Orp1p, a putative subunit of the fission yeast origin recognition complex (ORC), was found to be associated with chromatin-enriched insoluble components throughout the cell cycle. (elsevierpure.com)

This can either involve the replication of DNA in living organisms such as prokaryotes and eukaryotes, or that of DNA or RNA in viruses, such as double-stranded RNA viruses. (wikipedia.org)
In all other eukaryotes, clear consensus sequences for origins are elusive, perhaps indicating there are no cis elements to initiate replication in most of these cells and organisms. (biomedcentral.com)
Taken together, considerably greater flexibility in initiator-directed origin usage is found in eukaryotes than in prokaryotes. (biomedcentral.com)

The sequence analyses reveal that the k43 gene encodes the Drosophila homolog of the yeast origin recognition complex subunit 2 (Orc2p), a protein required for replication origin function and transcriptional silencing in yeast. (johnshopkins.edu)
Here, we study FOXD3, a transcriptional repressor enriched in pluripotent stem cells, and show that its repression of transcription upon S phase entry is critical to minimizing replication stress and preserving genome integrity. (bvsalud.org)
These results show how a transcriptional repressor can play a central role in maintaining genome integrity through the transient inhibition of transcription during S phase, enabling faithful DNA replication. (bvsalud.org)

Synthesis of daughter strands starts at discrete sites, termed replication origins, and proceeds in a bidirectional manner until all genomic DNA is replicated. (wikipedia.org)
During initiation, the replication machineries - termed replisomes - are assembled on DNA in a bidirectional fashion. (wikipedia.org)
It was found that (a) the ori site is located just to the left of gene O at approximately 80.2 ± 0.5% of the λ genome length (measured from the left terminus of the mature phage DNA), and (b) the orientation of replication is bidirectional, proceeding both to the left. (cshmonographs.org)

abstract = "DNA replication is an essential process in biology and its timing must be robust so that cells can divide properly. (elsevierpure.com)

These assembly loci constitute the start sites of DNA replication or replication origins. (wikipedia.org)
Here we have mapped sites of DNA replication initiation across the T. cruzi genome using Marker Frequency Analysis, which has previously only been deployed in two related trypanosomatids. (biomedcentral.com)

Here, we describe additional bioinformatic analyses showing that the previously identified GC-rich sequence elements form origin G-rich repeated elements (OGREs) that are present in 67% to 90% of the DNA replication origins from Drosophila to human cells, respectively. (crcm-marseille.fr)

Whole genome sequence analyses of NSCV1 and NSCV2, revealed that the origins of replication of Chr 1 and Chr 2 and associated genes are intact in the fused chromosome raising the question on how the two origins function without impeding each other's replication fork. (exordo.com)

To ensure complete and accurate duplication of the entire genome and the correct flow of genetic information to progeny cells, all DNA replication events are not only tightly regulated with cell cycle cues but are also coordinated with other cellular events such as transcription and DNA repair. (wikipedia.org)
Collision between replication and transcription is considered especially problematic, as each are catalyzed by large multiprotein machines, and can occur co-directionally, when the replication fork and transcription machinery are moving in the same direction, or on the leading strand and is head-on, when the fork and transcription are moving towards each other. (biomedcentral.com)
Tethers, composed of a trans-membrane and transcription repressor fusion protein bound to an array of operator sequences, can be placed up to 1 Mb from the origin with no loss of penetrance. (nih.gov)
Transcription and G-rich elements can independently associate with replication origin activity. (univ-amu.fr)
Coincident transcription and DNA replication causes replication stress and genome instability. (bvsalud.org)
Our findings reveal that TRAIP works in S-phase to prevent DNA damage at transcription start sites, caused by replication-transcription conflicts. (bvsalud.org)

Once priming is complete, DNA polymerase III holoenzyme is loaded into the DNA and replication begins. (wikipedia.org)
Verwijder na de synthese van de nieuwe strengen, RNase H of aanvullende varianten van DNA-polymerase, de primers en synthetiseer DNA in hun plaats. (jove.com)
Transient inhibition of RNA polymerase II in S phase reduces observed replication stress and cell cycle defects. (bvsalud.org)

Bacterial origins regulate orisome assembly, a nuclei-protein complex assembled on the origin responsible for unwinding the origin and loading all the replication machinery. (wikipedia.org)

Miller CLW and Winston F (2023) The conserved histone chaperone Spt6 is strongly required for DNA replication and genome stability. (yeastgenome.org)

These findings provide novel insights into mechanisms for pore-mediated origin unwinding, ATP/ADP-dependent regulation, and helicase loading of the initiation complex. (elsevierpure.com)
Om de replicatie te beginnen, wikkelt een enzym, helicase, de DNA-helix af en verbreekt de waterstofbruggen tussen de twee strengen. (jove.com)
Recombination and Pol ζ Rescue Defective DNA Replication upon Impaired CMG Helicase-Pol ε Interaction. (yeastgenome.org)

Berbenetz, N. M., Nislow, C. & Brown, G. W. Diversity of eukaryotic DNA replication origins revealed by genome-wide analysis of chromatin structure. (nature.com)
Nieduszynski, C. A., Knox, Y. & Donaldson, A. D. Genome-wide identification of replication origins in yeast by comparative genomics. (nature.com)
The "Early Origin Meta-Peaks" subtrack corresponds to early origins of replications that were identified by the genome-wide profiling of 5-bromo-2-deoxyuridine (BrdU). (wustl.edu)

Observing this process in large genomes like ours is difficult because eukaryotic cells have up to 50,000 replication start points per cell per cycle, and even the most commonly observed replication origin in the genome functions as such in just 10 percent of cells. (accela.eu)
DNA replication occurs once and only once per cell cycle mainly regulated by DNA replication initiation factors in eukaryotic cells. (intechopen.com)

Additionally, origin sequences commonly have high AT-content across all kingdoms, since repeats of adenine and thymine are easier to separate because their base stacking interactions are not as strong as those of guanine and cytosine. (wikipedia.org)
Replication initiation of the broad host range plasmid RK2 requires binding of the host-encoded DnaA protein to specific sequences (DnaA boxes) at its replication origin ( oriV ). (sri.com)
Analyses of autonomous replicating sequences have suggested that regions required for replication are very different from those in budding yeast. (elsevierpure.com)
In this paper, we propose the AT excursion method, which is a score-based approach, to quantify local AT abundance in genomic sequences and use the identified high scoring segments for predicting replication origins. (biomedcentral.com)
The AT excursion method will be a useful computational tool for identifying replication origins in a variety of genomic sequences. (biomedcentral.com)
7 ] develop the Oriscan algorithm to predict replication origins in the S. cerevisiae genome by searching for sequences similar to a training set of 26 known yeast origins pinpointed by site-directed mutagenesis. (biomedcentral.com)
Typically plasmids contain the minimum essential DNA sequences for this purpose, which includes a DNA replication origin, an antibiotic-resistance gene, and a region in which exogenous DNA fragments can be inserted. (addgene.org)

The origin of replication (also called the replication origin) is a particular sequence in a genome at which replication is initiated. (wikipedia.org)
Localization and sequence analysis of yeast origins of DNA replication. (nature.com)
In E. coli, the direction for orisome assembly are built into a short stretch of nucleotide sequence called as origin of replication (oriC) which contains multiple binding sites for the initiator protein DnaA (a highly homologous protein amongst bacterial kingdom). (wikipedia.org)
There are 11 DnaA binding sites/boxes on the E. coli origin of replication out of which three boxes R1, R2 and R4 (which have a highly conserved 9 bp consensus sequence 5' - TTATC/ACACA ) are high affinity DnaA boxes. (wikipedia.org)
During each G1 phase of two consecutive cell cycles, we observed the downstream repositioning of the origin-proximal +1 nucleosome and an increase in protected DNA fragments spanning the ARS consensus sequence (ACS) indicative of pre-RC assembly. (duke.edu)
All these algorithms exploit certain characteristic sequence features found around the replication origins. (biomedcentral.com)
The Z-curve of any given DNA sequence is a three-dimensional curve which uniquely represents the sequence so that unusual sequence compositional features, such as those around a replication origin, can sometimes be visually recognized. (biomedcentral.com)
In metazoa, DNA sequence elements involved in origin specification remain elusive. (univ-amu.fr)
We detect a shared G-rich DNA sequence signature that coincides with most core origins in both human and mouse gen-omes. (univ-amu.fr)
Computational algorithms show that core origins can be predicted, based solely on DNA sequence patterns but not on consensus motifs. (univ-amu.fr)
Nucleosome-directed replication origin licensing independent of a consensus DNA sequence. (yeastgenome.org)

Respiratory droplet (RD) transmission of influenza viruses requires efficient replication and release of infectious influenza particles into the air. (cdc.gov)

Different clones harboring different plasmids but having identical genetic structure, Tn 4401 , could be at the origin of the worldwide spread of this emerging resistance gene. (cdc.gov)
Plasmids can be distinguished by their incompatibility features (reflecting replication) and can therefore be grouped into several replicon (Inc) types [12]. (cdc.gov)

For less potent cell lines, protein levels are typically ~0.1 mG/L. The presence of the SV40 replication origin will result in high levels of DNA replication in SV40 replication permissive COS cells. (sigmaaldrich.com)

Replication is bi-directional and originates at a single origin of replication (OriC). (wikipedia.org)
Escherichia coli DnaA protein forms a homomultimeric complex with the replication origin (oriC). (elsevierpure.com)

The pre-replication complex (pre-RC) assembly or the DNA replication licensing is the first step in DNA replication initiation, characterized by the sequential recruitment of ORCs, Cdc6, Cdt1 and MCMs to the DNA replication origins to form the pre-RC at the end of mitosis ( Bell and Dutta 2002 ). (intechopen.com)

The replicator thereby specifies the location of replication initiation events, and the chromosome region that is replicated from a single origin or initiation event is defined as the replicon. (wikipedia.org)
Here, we examine pluripotent, primary, differentiating, and immortalized human cells, and demonstrate that a class of origins, termed core origins, is shared by different cell types and host~80% of all DNA replication initiation events in any cell population. (univ-amu.fr)

It is hypothesized that DNA stretching by DnaA bound to the origin promotes strand separation which allows more DnaA to bind to the unwound region. (wikipedia.org)

We infer that the stringent species and tissue specificities observed for papillomaviruses in vivo are not entirely due to direct restrictions on viral DNA replication. (elsevierpure.com)
We found that the Eurasian-origin gene segments contributed to efficient RD transmission of the pH1N1 virus likely by modulating the release of influenza viral RNA-containing particles into the air. (cdc.gov)
All viruses replicated well in the upper respiratory tract of infected ferrets, suggesting that factors other than viral replication are important for the release of influenza virus particles and transmission. (cdc.gov)

Dukaj L and Rhind N (2021) The capacity of origins to load MCM establishes replication timing patterns. (yeastgenome.org)

Fig. 3: Effects of Orc1 mutations on cell viability, complex formation, origin DNA binding and MCM loading. (nature.com)
As originally proposed in 1963, cells rely on two genetic elements to duplicate their genome: the replicator, a DNA region where replication begins (now named the replication origin), and the initiator, a protein or a protein complex that recognizes the replicator [ 1 ]. (biomedcentral.com)
In replication topologies more complex than replication from exactly one system to one other system, another problem can be that it is hard to avoid replicating replayed rows again. (postgresql.org)
We also found that the strongest correlation between chromatin occupancy at the ACS and origin efficiency occurred in early S phase, consistent with the rate-limiting formation of the Cdc45-Mcm2-7-GINS (CMG) complex being a determinant of origin activity. (duke.edu)
Rif1 controls DNA replication by directing Protein Phosphatase 1 to reverse Cdc7-mediated phosphorylation of the MCM complex. (yeastgenome.org)

The model postulates that a diffusible, trans-acting factor, a so-called initiator, interacts with a cis-acting DNA element, the replicator, to promote replication onset at a nearby origin. (wikipedia.org)

A chromatin immunoprecipitation assay demonstrated that Orp1p was preferentially localized at the ars2004 and ars3002 origins of the chromosome throughout the cell cycle, while SpMcm6p was associated with these origins only in the G 1 and S phases. (elsevierpure.com)
4 ] predict the replication origins for a number of bacterial and archaeal genomes by identifying some 7-mers and/or 8-mers whose orientation is preferentially skewed around the replication origins. (biomedcentral.com)

Enterovirus genome replication occurs at virus-induced structures derived from cellular membranes and lipids. (figshare.com)
Mitotic DNA synthesis (MiDAS) is an unusual form of DNA replication that occurs during mitosis. (bvsalud.org)
Initially, MiDAS was characterized as a process associated with intrinsically unstable loci known as common fragile sites that occurs after cells experience DNA replication stress (RS). (bvsalud.org)

Bryant JA and Aves SJ (2011) Initiation of DNA replication: functional and evolutionary aspects. (yeastgenome.org)

TYPES OF REPLICATION 6 Hypothetically, therecouldbethreepossible waysthat DNAreplication occur: Conservative replication: Bothparental strands stay together afterDNAreplication. (slideshare.net)
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied. (jove.com)

The replication of these viruses within cells relies on specialized membranous structures termed replication organelles (ROs) that form during infection but whose origin remains elusive. (figshare.com)
We have also applied the AT excursion method to two other families of double stranded DNA viruses, the poxviruses and iridoviruses, of which very few replication origins are documented in the public domain. (biomedcentral.com)
In Domingo E, Webster RG, Holland JJ (eds) Origin and evolution of viruses. (springer.com)

yet, it has been shown that many organisms use preferred genomic regions as origins. (wikipedia.org)
DNA replication initiates from multiple genomic locations called replication origins. (univ-amu.fr)
Our results demonstrate that, despite an attributed stochasticity, core origins are chosen from a limited pool of genomic regions. (univ-amu.fr)
In this chapter, we mainly discuss the coordination regulations between DNA replication initiation and other cell cycle events that ensure genomic integrity. (intechopen.com)
The precise regulations of pre-RC protein levels and assembly are effective ways to prevent reassembly of de novo MCM2-7 onto the replicated origins to re-license and re-replicate the genomic DNA in the subsequent phases of the same cell cycle ( Figure 1) . (intechopen.com)

Incomplete, erroneous, or untimely DNA replication events can give rise to mutations, chromosomal polyploidy or aneuploidy, and gene copy number variations, each of which in turn can lead to diseases, including cancer. (wikipedia.org)
Gene expression patterns, assayed by RNA sequencing, show that tethering induces global supercoiling changes, which are likely incompatible with replication initiation. (nih.gov)
Immortalization through oncogenic gene expression, but not normal cellular differentiation, results in increased stochastic firing from heterochromatin and decreased origin density at TAD borders. (univ-amu.fr)
The ancestral ORC1b gene, present before the partial duplication of the Arabidopsis genome, has retained the canonical function in DNA replication. (bvsalud.org)
Eurasian-origin gene segments contribute to the transmissibility, aerosol release, and morphology of the 2009 pandemic H1N1 influenza virus. (cdc.gov)
Additionally, the pleomorphic phenotype of the pH1N1 virus is dependent upon the Eurasian-origin gene segments, suggesting a link between transmission and virus morphology. (cdc.gov)

Loss of FOXD3-interacting histone deacetylases induces replication stress, while transient inhibition of histone acetylation opposes it. (bvsalud.org)

According to the usage, origins have been classified as constitutive (fired at same position in different cells of a population), flexible (fired stochastically in different cells) and dormant (fired as consequence of replication stress) [ 5 ]. (biomedcentral.com)

However, how this nucleosome organization is established and whether this organization is required for replication remain unknown. (nature.com)
Here, using genome-scale biochemical reconstitution with approximately 300 replication origins, we screened 17 purified chromatin factors from budding yeast and found that the ORC established nucleosome depletion over replication origins and flanking nucleosome arrays by orchestrating the chromatin remodellers INO80, ISW1a, ISW2 and Chd1. (nature.com)
Fig. 1: ORC is a master regulator of nucleosome organization at origins of replication. (nature.com)

Although it has been recognized for several decades that chromosome structure regulates the capacity of replication origins to initiate, very little is known about how or if cells actively regulate structure to direct initiation. (nih.gov)
DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7. (yeastgenome.org)

A key prerequisite for DNA replication is that it must occur with extremely high fidelity and efficiency exactly once per cell cycle to prevent the accumulation of genetic alterations with potentially deleterious consequences for cell survival and organismal viability. (wikipedia.org)
Genome replication is responsible for accurate transmission of genetic information through cell division cycles. (biomedcentral.com)
Then, when cells reach S-phase, a set of enzymatic and regulatory factors activate some, but not all, origins, which are differently used depending on the cell types and stages of development, and even in different cells of the same population. (biomedcentral.com)
We recently reported the identification and characterization of DNA replication origins (Oris) in metazoan cell lines. (crcm-marseille.fr)
All cells must finish DNA replication before they can proceed for cell division. (wikipedia.org)
We report that a localized inducible protein tether between the chromosome and cell membrane in E. coli cells imparts a rapid and complete block to replication initiation. (nih.gov)
Initiation of chromosomal replication and its cell cycle-coordinated regulation bear crucial and fundamental mechanisms in most cellular organisms. (elsevierpure.com)
Origins of enterovirus replication organelles established by whole-cell electron microscopy. (figshare.com)
Whole-cell data sets further revealed striking contact regions between ROs and lipid droplets that may represent a route for lipid shuttling to facilitate RO proliferation and genome replication. (figshare.com)
Scholars@Duke publication: Cell-Cycle-Dependent Chromatin Dynamics at Replication Origins. (duke.edu)
Origins of DNA replication are specified by the ordered recruitment of replication factors in a cell-cycle-dependent manner. (duke.edu)
These results provide insights into cell-cycle-regulated chromatin dynamics and how they relate to the regulation of origin activity. (duke.edu)
[ 4 ] One is that a single cell escapes the controls of normal cell replication, forms a tumor at the site of origin, and the tumor cells ultimately metastasize to other organs, but the original tumor is too small to be detected at the time of metastasis. (medscape.com)
Thus, regardless of their cell of origin, CUPs share similarities and can be considered a specific entity. (medscape.com)

A number of computational methods have been developed for predicting replication origins in bacterial, archaeal, and eukaryotic genomes. (biomedcentral.com)

We have examined the activity of the two origins using next generation sequencing based marker frequency analyses. (exordo.com)
Replication analyses across independent drug and CRISPR genetic screening data sets reveal hundreds of robust associations, which are provided as a resource for drug repurposing guided by mutational signature markers. (nature.com)

Acutely deleting Foxd3 leads to immediate replication stress, G2/M phase arrest, genome instability and p53-dependent apoptosis. (bvsalud.org)
In this review, we introduce the causes of RS, regions of the human genome known to be especially vulnerable to RS, and the strategies used to complete DNA replication outside of S phase. (bvsalud.org)

 Originrecognitioncomplex(ORC)   Actsasthe initiator of eukaryotic DNA replication. (slideshare.net)

DNA replication in trypanosomatids operates in a uniquely challenging environment, since most of their genomes are constitutively transcribed. (biomedcentral.com)
Studies looking into replication origins have largely focused on simple organisms with smaller genomes. (accela.eu)
For example, Lobry [ 3 ] employs the GC skew plot to predict replication origins and terminus in bacterial genomes. (biomedcentral.com)
Zhang and Zhang [ 5 ] use the Z-curve method successfully to identify several replication origins in bacterial and archaeal genomes. (biomedcentral.com)

Although the specific replication origin organization structure and recognition varies from species to species, some common characteristics are shared. (wikipedia.org)

e.g. inflammation, genotoxicity, and This chapter focuses on issues as- ellers, growth factors, growth factor epigenetic alterations) and can have sociated with the understanding and receptors, signal transducers, and both genetic and epigenetic origins. (who.int)

Anatomy20

Organisms39

Diseases3

Chemicals and Drugs92

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes98

Information Science4

Health Care1

Chromosome replication6

Chromosomes6

Bacteria3

Proteins12

Forks5

Saccharomyces1

Required for autonomous2

Putative3

Eukaryotes3

Transcriptional3

Bidirectional3

Abstract1

Sites of DNA replication2

Drosophila1

Genes1

Transcription6

Polymerase3

Machinery1

20231

Helicase3

Genome-wide3

Eukaryotic cells2

Sequences7

Sequence11

Efficient replication1

Plasmids2

SV401

OriC2

Cdt11

Initiation events2

DnaA1

Viral3

20211

Complex5

Replicator1

Preferentially2

Occurs3

Functional and evolutionary1

Strands2

Viruses3

Genomic5

Gene6

Transient1

Dormant1

Nucleosome3

Regulates2

Cell14

Computational1

Analyses2

Phase2

Initiator1

Genomes4

Organization1

Genetic1