Replication of recombinant recombinant proteins. Medical search. Frequent questions

These fragments are then inserted into cloning vectors , such as bacterial plasmids or bacteriophages, which transfer the recombinant DNA to suitable host cells, such as the bacterium E. coli. (encyclopedia.com)
Single Stranded DNA Binding Protein, E. coli is available at Gentaur for Next week delivery. (telospub.com)
PCBP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 186 amino acids (1-163) and having a molecular mass of 19.6 kDa. (prospecbio.com)
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. (gentaur.com)

The iNCOVACC is a recombinant replication-deficient adenovirus vectored vaccine with a pre-fusion stabilized spike protein. (livemint.com)
Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 candidate vaccine delivered by a replication-defective recombinant adenovirus vector. (emmes.com)
Here, we report the safety, tolerability, and immunogenicity of a replication-defective recombinant adenovirus serotype 5 (rAd5) vector HIV-1 candidate vaccine. (emmes.com)
We also show that recombinant adenovirus vaccination induced T cell responses to the same epitopes, and led to memory T cell differentiation. (biomedcentral.com)
T cells primed by these recombinant adenovirus vaccines expanded after PPRV challenge and probably contributed to protection. (biomedcentral.com)
These data validate the use of recombinant adenovirus expressing PPRV genes as DIVA strategies to control this highly contagious disease. (biomedcentral.com)
Recombinant adenovirus vaccines should therefore aim at replicating the naturally occurring PPRV immunity. (biomedcentral.com)

Our selection of CMV vectors provides options for transient or stable expression of 3xFLAG ® fusion proteins. (sigmaaldrich.com)
Vectors containing the preprotrypsin leader (PPT) sequence direct secretion of FLAG fusion proteins into the culture medium for purification using ANTI-FLAG antibodies, resins, and plates. (sigmaaldrich.com)
It is a bivalent vaccine containing two recombinant, replication-deficient lymphocytic choriomeningitis virus (rLCMV) vectors, one expressing the pp65 protein and one expressing the gB protein of human cytomegalovirus (HCMV). (pharmaceutical-technology.com)

Prions are abnormal, transmissible pathogenic agents that induce abnormal folding of specific, normal cellular proteins. (medicalnewstoday.com)
Hookipa's proprietary arenavirus-based technologies, VaxWave, a replication-deficient viral vector, and TheraT, a replication-attenuated viral vector, are designed to induce antigen specific cytotoxic T cells and pathogen-neutralizing antibodies for prophylactic and therapeutic purposes activating body's immune response. (pharmaceutical-technology.com)
Little is known of the T cell repertoire these recombinant vaccines induce. (biomedcentral.com)
The potential of BromAc on SARS-CoV-2 spike and envelope proteins stabilized by disul?de bonds was examined and found to induce the unfolding of recombinant spike and envelope proteins by reducing disul?de stabilizer bridges. (newstarget.com)

The study began with the generation of recombinant (r)SA11 rotaviruses, into which segment 7 modified RNAs were introduced. (news-medical.net)

dsDNA viruses constitute Group I in the Baltimore classification system and use methods of replication and transcription that are broadly similar to those of larger organisms such as bacteria. (wikipedia.org)
The recombinant HVT-VP2 viruses were stable and showed continuous expression of VP2 even after several passages in cell culture. (thepoultrysite.com)
The resulting rSA11/NSP3-CoV2/S viruses contain 12% more material than rSA11/wt (wildtype recombinant rotavirus). (news-medical.net)
The rSA11/NSP3-CoV2/S viruses produced smaller plaques and lower viral titers in cell culture than the wildtype, perhaps because the RNA elongation time required for transcription of the segment 7 dsRNAs is increased during the replication of the virus, or the longer translation time to synthesize proteins encoded from these RNAs. (news-medical.net)
These replacements also led to production of infective viruses that replaced the acidic residues introduced (E) by nonpolar amino acids, indicating that preservation of the hydrophobic interface is essential for virus replication. (asm.org)
RNA viruses may escape acquired humoral and cellular immune responses by mutations in protective antigenic epitopes (e.g., avian influenza viruses), while accessory nonstructural proteins or multifunctional structural proteins interfere with the interferon system (e.g. (imperial.ac.uk)
viruses failing to express either protein are unresponsive to reactivation stimuli. (bvsalud.org)
We generated recombinant viruses lacking UL135 that expressed a stabilized variant of UL136p33. (bvsalud.org)
Retroviruses are enveloped RNA viruses defined by their mechanism of replication via reverse transcription to produce DNA copies that integrate into the host cell's genome. (msdmanuals.com)

Baltimore classification base pair (bp) cap cap snatching capsid The outer shell of protein that encloses and protects the genetic material of a virus. (wikipedia.org)
Viral genetic factors are proteins such as Tat, Vif and Rev. Human genetic factors associated with a better clinical outcome are proteins such as APOBEC, langerin, tetherin and chemokine receptor 5 (CCR5) and HLA B27, B57, DRB1{*}1303, KIR and PARD3B. (uni-muenchen.de)
The recombinant rotavirus containing a cassette of foreign genetic material encoding the NSP3 ORF, a translational element responsible for translating this inserted gene, and the gene encoding the spike protein . (news-medical.net)
Consideration of Mendelian genetics allows an overview of DNA replication, gene transcription, protein synthesis, mRNA splicing that informs the opportunity to discuss control of gene expression, recombinant DNA technology and genetic disease. (iuhs.edu)
Replication studies identify several genetic variants in Arabs with obesity- linked diabetes. (who.int)

AAV is a nonhuman pathogen of the Parvoviridae family possessing a single-stranded DNA genome (4.7 kb) with two open reading frames, rep (for replication) and cap (encodes capsid proteins), flanked by two symmetric inverted terminal repeats. (molvis.org)
The viral particle is composed of a protein capsid that contains a positive-sense RNA molecule of about 8,500 nucleotides that is infectious and encodes a single polyprotein, which is processed in infected cells by cis - and trans -acting viral proteases ( 55 ) to yield different polypeptide precursors and the mature viral proteins ( 9 , 62 ). (asm.org)
The viral genome encodes four structural capsid proteins (VP1 to VP4) and seven nonstructural (NS) proteins, the leader Lb/ab protease, and proteins encoded in the P2 (2B and 2C) and P3 (3A, 3B, 3C, and 3D) regions ( 9 ). (asm.org)
By contrast, UL136 is expressed with later kinetics and encodes multiple proteins with differential roles in latency and reactivation. (bvsalud.org)

Several PAR-binding modules orchestrate the relocation of DDR-associated factors in addition to the accumulation of intrinsically disordered proteins through an intracellular liquid demixing mechanism 11 , 12 . (nature.com)
The flavivirus nonstructural protein 1 (NS1) functions in genome replication as an intracellular dimer and in immune system evasion as a secreted hexamer. (nih.gov)
Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). (novusbio.com)
NS proteins are involved in crucial aspects of the viral cycle and pathogenesis, such as rearrangements of intracellular membranes required for endomembrane recruitment and the lysis of host cells ( 1 , 12 , 14 , 18 , 73 ). (asm.org)
in poliovirus (PV), the interaction between the RNA replication complex and intracellular membranes appears to be accomplished by proteins 3A and 2C, which have membrane-binding properties ( 11 , 60 ). (asm.org)
On the other hand, 3AB presumably anchors 3B in intracellular membranes originated de novo during the early steps of RNA replication, where uridylylated 3B primes the synthesis of nascent viral RNAs ( 2 , 37 , 68 , 69 ). (asm.org)
We demonstrated that CP does not affect the translation efficiency from genomic (gRNA) or subgenomic RNA (sgRNA), the intracellular distribution of the non-structural proteins (NSP), or sgRNA synthesis. (cdc.gov)

Such enhancement can occur by viral capsid proteins (CP) alone in trans. (cdc.gov)

rCP was found to restore replication of a few mutants in NSP but failed to fully restore replicons known to have defects in the positive-strand RNA synthesis. (cdc.gov)
These results suggest that the exogenous RuV CP increases efficiency of early viral genome replication by modulating the stage(s) prior to and/or at the initiation of negative-strand RNA synthesis, possibly through a general mechanism such as protecting viral RNA. (cdc.gov)
1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)

In Ebolavirus -infected cynomolgus macaques, use of human recombinant interferon alfa-2b in conjunction with hyperimmune equine immunoglobulin G (IgG) delayed but did not prevent death. (medscape.com)

Poly(ADP-ribose) polymerase-1 (PARP-1) is an abundant and ubiquitous nuclear protein that uses NAD + to synthesize a multibranched polyanion composed of ADP-ribose moieties, giving rise to poly(ADP-ribose) (PAR), onto itself or a variety of target proteins. (nature.com)
Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, CBP and its paralogue, p300, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase β and nuclear import factors. (enzolifesciences.com)
These phenotypes appear to result, at least in part, from impaired interactions of the mutant β159 clamp protein with the replicative DNA polymerase, Pol III ( 38 ). (asm.org)
In contrast to these three Pols, which under certain conditions impede growth of the dnaN159 strain ( 29 , 39 , 40 ), presumably by impairing DNA replication, the catalytic DNA polymerase activity of Pol I ( polA ) is essential for viability of the dnaN159 strain ( 38 ). (asm.org)
Following nasopharyngeal swab (NP) collection, VDRV was detected by real-time polymerase chain reaction (RT-qPCR) and sequencing, and live, replication-competent VDRV was isolated in cell culture. (cdc.gov)

DESCRIPTION (provided by applicant): With its ability to secrete high levels of recombinant proteins, the yeast Pichia pastoris is extensively used for protein manufacturing. (sbir.gov)
However, as actual yields vary widely for individual proteins, GlycoFi is focusing on the improvement of protein yields in P. pastoris as part of the company's long term mission to develop yeast-based protein production technology for manufacturing therapeutic proteins with human glycosylation structures. (sbir.gov)
In Phase I of this project a strategy was developed and tested using the model protein alpha 1-antitrypsin for overcoming bottlenecks in yeast secretion that result in less than optimal yields. (sbir.gov)
Proposed Commercial Application: GlycoFi's Humanized Yeast(tm) enables the study of glycosylation structure-function relationships and creates the ability not only to pinpoint the specific glycoforms that optimize the bioactivity, pharmacokinetics and overall efficacy of a therapeutic protein, but also to manufacture them at low cost with a high degree of uniformity. (sbir.gov)
Histone H3.3, an H3 variant that is found in all eukaryotes from yeast to human, is replication and cell cycle phase-independent and is the most common H3 in non-dividing cells. (neb.com)
In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. (bdbiosciences.com)

Global Virus Network (GVN) group-specific antigen helical helper dependent virus helper virus Any virus which aids or allows the replication of a coinfecting virus that is incapable of replicating on its own. (wikipedia.org)

Foot-and-mouth disease virus (FMDV) nonstructural protein 3A plays important roles in virus replication, virulence, and host range. (asm.org)

We here use a purified recombinant mitochondrial transcription system and demonstrate that conserved sequence box II is a sequence-dependent transcription termination element in vitro. (nih.gov)
p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. (enzolifesciences.com)
Then, mRNA and protein expression levels of CD34 in hepatocellular carcinoma (HCC) samples were detected via real-time quantitative reverse transcription PCR (qRT-PCR) and immunohistochemical (IHC), respectively. (bvsalud.org)

antigenic drift A gradual change in the antigenicity of a virus arising from the accumulation of mutations in the genes that code for surface proteins, which may result in new strains of the virus that are not as effectively inhibited by the same host antibodies that prevented infection by the original strain. (wikipedia.org)

Understanding the role of the LC in FCV replication is important because there are currently no antiviral drugs available for FCV and there are numerous reports of vaccine failure. (umd.edu)
Is it possible to mitigate the damaging effects of covid "vaccine" spike proteins through simple nutrition? (newstarget.com)
A tissue-culture system using recombinant DNA technology was recently developed and has advanced the scientific knowledge base considerably, including early forays into vaccine development. (medscape.com)

Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication of genes. (il-12p40.com)

Since Pol III replication is abated in strains bearing the dnaN159 allele ( 14 , 35 , 38 ), due to the impaired ability of β159 to interact with the α catalytic subunit of Pol III ( 38 ), we hypothesized that the requirement for Pol I function in the dnaN159 strain might stem from its ability to augment Pol III function in DNA replication. (asm.org)

PCBP1 and PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promotes poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. (prospecbio.com)
By applying deletional mutagenesis and scanning alanine mutagenesis to the LC coding sequence I identified regions and conserved residues critical for viral replication and virus spread. (umd.edu)
An examination of the nucleic acid sequence alignment of 48 full-length rubella virus genomes revealed that the 5' terminus of the genome is more conserved than the commonly used detection windows for rubella virus RNA located in the E1 protein coding region, suggesting that the 5' terminus could be a target for improving detection of all rubella virus genotypes. (cdc.gov)

VP2 protein was expressed at high levels as determined by immunofluorescence of CVRM-VP2 infected chicken embryo fibroblasts. (thepoultrysite.com)
VP2 protein was expressed at similar levels regardless of the cloning location, as determined by immunofluorescence of HVT-VP2 infected chicken embryo fibroblasts. (thepoultrysite.com)
Recombinant Human C-C chemokine receptor type 8(CCR8) referenced in "The chemokine CCL1 triggers an AMFR-SPRY1 pathway that promotes differentiation of lung fibroblasts into myofibroblasts and drives pulmonary fibrosis", Immunity , 2021. (cusabio.com)
Stabilizing UL136p33 did not impact the replication of the UL135 mutant virus in fibroblasts. (bvsalud.org)

These encoded sequences for the non-structural protein NSP3 and some parts of the viral spike. (news-medical.net)
Recombinant Human Novel Coronavirus Spike glycoprotein(S), partial (Active) referenced in "Insights into neutralizing antibody responses in individuals exposed to SARS-CoV-2 in Chile", Science Advances , 2021. (cusabio.com)
Recombinant Human Novel Coronavirus Spike glycoprotein(S) (D614G), partial (Active) referenced in "Screening of potent neutralizing antibodies against SARS-CoV-2 using convalescent patients-derived phage-display libraries", Cell discovery , 2021. (cusabio.com)
Australian researchers looked at two common nutritional supplements - bromelain and acetylcysteine (BromAc) - that are capable of unfolding the structures that hold spike proteins together, effectively neutralizing them. (newstarget.com)
Severe acute respiratory syndrome coronavirus, also known as SARS-CoV-2, is demarcated by a spike glycoprotein and envelope protein that contains disulfide bridges for stabilization. (newstarget.com)
A BromAc treatment, the team found, is capable of "synergistic action against glycoproteins" in that the nutritional duo breaks apart the glycosidic linkages and disulfide bonds, reducing or even eliminating the ability of spike proteins to cause covid infection. (newstarget.com)
These same spike proteins, just to clarify, are present inside all of the so-called "vaccines" currently available to the public. (newstarget.com)
Some would argue that the only source of these spike proteins is the injections, and that covid itself does not exist since the alleged virus has never been isolated . (newstarget.com)
We sought to determine the effect of BromAc on the spike and envelope proteins and its potential to reduce infectivity in host cells," the paper reads. (newstarget.com)
Recombinant spike and envelope SARS-CoV-2 proteins were disrupted by BromAc. (newstarget.com)
Spike and envelope protein disulfide bonds were reduced by Acetylcysteine. (newstarget.com)
Is BromAc a natural solution for covid jab spike protein damage? (newstarget.com)
In other words, combining these two nutrients at the levels depicted in the study led to destabilization and unraveling of covid spike proteins, which is good news for everyone who got jabbed, as well as for those who may have had spike protein "shed" on them from a fully vaccinated person. (newstarget.com)
Still, the results are "encouraging," the researchers say, pointing to a natural solution for covid spike proteins that otherwise outsmart most other potential remedies. (newstarget.com)
BromAc also showed an inhibitory effect on wild-type and spike mutant SARS-CoV-2 by inactivation of its replication capacity in vitro . (newstarget.com)
1 , 2 , 3 The viral envelope of coronaviruses is composed of four major viral structural protein components: spike (S) protein, membrane (M) protein, nucleocapsid (N), and the envelope (E) protein. (ispe.org)
Provides instructions the body uses to build a version of a Ad26 vector, encoding a stabilized variant harmless piece of a protein from the virus that virus unrelated of the SARS-CoV-2 Spike (S) protein causes COVID-19. (cdc.gov)

The objective of this study was to determine the potential of a normal host cellular prion protein (PrPC). (cdc.gov)
Addressing the need to define steadfast analytical performance criteria for prion amyloid seeding assays (ASAs), researchers developed a method to measure prion protein conversion time (from normal cellular form to prion form) by a combination of statistical analyses to obtain a prion-detecting ASA with a known degree of confidence. (medicalnewstoday.com)
Prion disease occurs when the normal cellular form of prion-related protein converts or conformationally changes to the disease form. (medicalnewstoday.com)
Suppression of this enzyme increases cellular 2-5A levels and decreases viral replication in cultured small-airway epithelial cells. (creativebiomart.net)
PCBP1 along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. (prospecbio.com)
Immunoprecipitation of recombinant LC in FCV infected cells identified the cellular protein annexin A2 as a binding partner, providing a potential mechanism for the cell-rounding phenotype observed in transient expression experiments. (umd.edu)
The nitty gritty of cellular organisation is examined from membrane structure/function, to bio-molecular structures of proteins, nucleic acids, and lipids. (iuhs.edu)

Shrimp Alkaline Phosphatase, Recombinant is available at Gentaur for Next week delivery. (telospub.com)
High specific activity, heat-labile alkaline phosphatase purified from a recombinant source. (telospub.com)
Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. (novusbio.com)

Enhanced replication of rubella virus (RuV) and replicons by de novo synthesized viral structural proteins has been previously described. (cdc.gov)

With a protein coat and lipid bilayer, these mammalian virus-based reference materials resemble the complexity of virus targets found in clinical samples. (seracare.com)
Embedded in the viral envelope are 180 copies of the E and M proteins that span through the lipid bilayer. (medchemexpress.com)

Since Pol I is a multifunctional protein that participates in DNA replication, as well as numerous DNA repair pathways, several possibilities exist, including Okazaki fragment maturation ( 21 , 31 ) and single-stranded DNA (ssDNA) gap repair ( 16 , 22 ). (asm.org)

Additionally, the interaction of PCNA with Pol δ is critical for coordinating its transient replacement by other PCNA partner proteins. (nature.com)
The G174A substitution appears to impair the ability of a hydrophobic cleft in β to interact with the eubacterial clamp-binding motif (QL[S/D]LF) that is conserved in most partner proteins reported to interact with the β clamp ( 8 , 38 ). (asm.org)

The strong human cytomegalovirus (CMV) promoter regulatory region drives constitutive protein expression levels as high as 1 mG/L in COS cells. (sigmaaldrich.com)
Full-length human recombinant MLH was expressed as a maltose binding-MLH fusion protein, affinity purified, and used as immunogen. (bdbiosciences.com)
These proteins form a protective outer layer that controls the entry of the virus into human cells. (medchemexpress.com)

is used as a model to study basic mechanisms of replication of caliciviruses because it grows well in cell culture and it has a reverse genetics system. (umd.edu)
In order to study the role of the LC in virus replication I employed the reverse genetics system to generate recombinant full-length FCV genomes, and performed transient expression experiments with the LC alone. (umd.edu)
Using reverse genetics , recombinant rotaviruses of species A have been created. (news-medical.net)
Earlier, this was shown to be susceptible to modification using reverse genetics, whereby two separate proteins could be expressed by this open reading frame (ORF). (news-medical.net)

The nonstructural proteins play roles in viral replication and assembly. (medchemexpress.com)

The S protein is a type 1 transmembrane glycoprotein that is expressed on the surface of coronaviruses (CoV) and is responsible for receptor binding and virion entry into the cells. (ispe.org)

Treatment with Acetylcysteine on the envelope protein did not alter the protein, whereas treatment with Bromelain at 50 and 100µ g/mL and BromAc at 50 and 100µ g/20 mg/mL also resulted in near complete and complete fragmentation, respectively," the paper further explains. (newstarget.com)

It is our expectation that this recombinant vector can provide protection against IBD and highly virulent MD challenge, concluded Dr Lupiani. (thepoultrysite.com)
The use of turkey herpesvirus (HVT) as a viral vector to express immunogenic proteins has been shown to be successful in the poultry industry. (thepoultrysite.com)
Dr Reddy said it was his expectation that this recombinant vector can provide protection against IBD and Marek's disease challenge. (thepoultrysite.com)

The amino acids are interconnected in such a manner that they form a surface into which the proteins and other membrane constituents are inserted and secured. (politicalforum.com)
These proteins are necessary for the transport through the cell membrane of many types of nutrients and waste materials. (politicalforum.com)
The transport proteins of the membrane become locked, and the membranes become less permeable. (politicalforum.com)
and the E protein is a minor constituent of virions and is an integral membrane protein. (ispe.org)

Loss of function of DNA repair enzymes can lead to an accumulation of replication errors, resulting in a mutated phenotype. (bdbiosciences.com)

Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. (bdbiosciences.com)

PrPC is a susceptibility to infection of 5 cell lines used or proposed for nonessential protein but, at least in mice and cows, must be manufacture of biological products, as well as other lines. (cdc.gov)
Most cases of vCJD are attributed to TSE-associated prion protein (PrPTSE) and TSE infectivity exposure to contaminated beef products ( 1-3 ). (cdc.gov)
No food proteins like eggs or egg products, gluten, peanuts, tree nuts, nut products, or any nut byproducts (COVID-19 vaccines are not manufactured in facilities that produce food products). (cdc.gov)

Compatible with multiplexed RT-PCR and NGS-based assays, AccuPlex custom recombinant virus materials are constructed with a replication-defective mammalian virus, producing a safe, non-infectious material (Figure 1). (seracare.com)

A combinatorial library/high throughput screening methodology was designed to optimize translocation of the nascent peptide into the endoplasmic reticulum (ER) and protein folding within the ER. (sbir.gov)
When expressed as a recombinant protein in transfected cells, PV 3A cofractionates with endoplasmic reticulum markers ( 66 ), and its single transient expression can disrupt the secretory apparatus ( 23 ) and decrease major histocompatibility complex (MHC) class I expression ( 22 ). (asm.org)

Can be used to study CBP kinetics and regulation, for screening for inhibitors or activators and for radiolabeling of proteins or peptides with, for example [ 3 H]-Acetyl CoA. (enzolifesciences.com)
Replacements L38E and L41E, involving charge acquisition at residues predicted to contribute to the hydrophobic interface, reduced the dimerization signal in the protein ligation assay and prevented the detection of dimer/multimer species in both transiently expressed 3A proteins and in synthetic peptides reproducing the N terminus of 3A. (asm.org)

PCBP1 is a single-stranded nucleic acid binding protein that binds preferentially to oligo dC. (prospecbio.com)

The majority of the drug companies designed vaccines and therapies that targeted the S protein of the SARS-CoV-2 virus. (ispe.org)

Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. (novusbio.com)

Recombinant NS1 binds to lipid bilayers and remodels large liposomes into lipoprotein nanoparticles. (nih.gov)

For less potent cell lines, protein levels are typically ~0.1 mG/L. The presence of the SV40 replication origin will result in high levels of DNA replication in SV40 replication permissive COS cells. (sigmaaldrich.com)
Nucleoside analogue inhibitors of the cell-encoded enzyme S-adenosylhomocysteine hydrolase (SAH) have been shown to inhibit Zaire ebolavirus replication in adult BALB/c mice infected with mouse-adapted Ebola virus. (medscape.com)
Replication of picornaviruses occurs associated to cell endomembranes that are recruited during viral infection ( 25 ). (asm.org)
5 Minor differences in CoV S protein structure and function correlates with striking changes in CoV tropism (ability to infect different cell types) and virulence. (ispe.org)

The core of the virus is the nucleocapsid, a structure that is made of the viral genome along with C proteins. (medchemexpress.com)

While UL138 is pro-latency, restricting virus replication in CD34+ hematopoietic progenitor cells (HPCs), UL135 overcomes this restriction and is required for reactivation. (bvsalud.org)
This protein causes an to the COVID-19 immune response that helps protect the body virus from getting sick with COVID-19 in the future. (cdc.gov)
It is not clear whether the CP in the virus particles, i.e., the exogenous CP, modulate viral genome replication. (cdc.gov)
Lassa fe- Lassa virus in many more districts and states in en- ver is endemic in West Africa and has been reported demic countries of the West African sub-region and from Sierra Leone, Guinea, Liberia, and Nigeria4-7. (folkhalsomyndigheten.se)

The expression of NSP3 along with one or more fluorescent tags allows the expression of a foreign protein, while NSP3 remains a functional, stable dimerizing product. (news-medical.net)

It was also recently shown that PARP-1 is a sensor of unligated Okazaki fragments during DNA replication 16 and cells deficient in ribonucleotide excision repair are sensitized to PARP inhibition 17 . (nature.com)

A molecular model of the FMDV 3A protein, derived from the nuclear magnetic resonance (NMR) structure of the poliovirus 3A protein, predicted a hydrophobic interface spanning residues 25 to 44 as the main determinant for 3A dimerization. (asm.org)

AAV2 containing cDNA encoding enhanced green fluorescent protein (GFP), under the control of a chicken β-actin promoter, was delivered by intravitreal injection to adult mice in conjunction with enzymes including collagenase, hyaluronan lyase, heparinase III, or chondroitin ABC lyase. (molvis.org)

Purified recombinant protein of mouse full-length phosphodiesterase 12 (Pde12), with C-terminal MYC/DDK tag, expressed in HEK293T cells. (creativebiomart.net)

However, in the context of infection in HPCs, stabilization of UL136p33 strikingly compensated for the loss of UL135, resulting in increased replication in CD34+ HPCs and in humanized NOD-scid IL2RÎ³cnull (huNSG) mice. (bvsalud.org)

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