The present study was undertaken to determine the molecular location of the origin of autonomous λ DNA replication ( ori ), the orientation of the in situ replication of the induced but not excised λ prophage, and the effect of mutations in the phage or host genome on λ DNA replication. (cshmonographs.org)
It was found that (a) the ori site is located just to the left of gene O at approximately 80.2 ± 0.5% of the λ genome length (measured from the left terminus of the mature phage DNA), and (b) the orientation of replication is bidirectional, proceeding both to the left. (cshmonographs.org)
After completing a degree in 1967, he entered Massachusetts Institute of Technology (MIT), where he studied the molecular biology of the lambda phage. (wikipedia.org)
As a graduate student, Herskowitz made major contributions to the scientific understanding of regulation of gene expression in Lambda phage. (wikipedia.org)
Lambda phage was the first organism whose regulatory strategies became well understood, due in part to Herskowitz' pioneering work. (wikipedia.org)
Herskowitz further described the products of the N, cII, and cIII genes, positive regulation of cII and cIII, and N's central role as a positive regulator for rightward and leftward transcription in lambda phage. (wikipedia.org)
They determined the relationship between Salmonella phage P22 and coliphage lambda, and discovered that bacteriophage genomes had a modular structure. (wikipedia.org)
Twenty eight (46.7%) of the proteins encoded by this virus exhibit sequence similarity to coliphage T7 proteins while 34 (81.0%) are similar to proteins of Pseudomonas phage gh-1. (biomedcentral.com)
That this phage is closely related to Pseudomonas putida phage gh-1 and coliphage T7 places it in the "T7-like viruses" genus of the subfamily Autographivirinae within the family Podoviridae . (biomedcentral.com)
After prophage induction or after infection, replication of λ DNA becomes autonomous and much more rapid than that of the host genome. (cshmonographs.org)
The genome of coliphage T7 has 56 genes encoding potential proteins [ 8 ], of which 35 have known function or functions. (biomedcentral.com)
Genes1
Herskowitz also worked with Costa Georgopoulos on host genes that control DNA replication and host mutants with virus growth defects. (wikipedia.org)
Bacterial1
In the lysogenic, integrated state coliphage λ DNA is replicated as a part of the bacterial chromosome. (cshmonographs.org)
Escherichia6
the carboxy-terminal domain of the transcription factor escherichia coli nusa, nusactd, interacts with the protein n of bacteriophage lambda, lambdan, and the carboxyl terminus of the e. coli rna polymerase alpha subunit, alphactd. (liverpool.ac.uk)
a two-stage continuous culture of escherichia coli in combination with a bacteriophage lambda system was performed in order to overcome the intrinsic plasmid instability that is frequently observed in recombinant fermentation. (liverpool.ac.uk)
heterologous escherichia coli or bacteriophage lambdadna fragments of various sizes were inserted into vectors based on either the rolling-circle-type plasmid pwv01 or the theta-type plasmid pambeta1. (liverpool.ac.uk)
We report the identification and functional analysis of nine genes from Gram-positive and Gram-negative bacteria and their phages that are similar to lambda (lambda) bet or Escherichia coli recT. (nih.gov)
Plasmid formation after lambda bacteriophage infection of Escherichia coli-Salmonella typhosa hybrids. (wikidata.org)
Salmonella1
They determined the relationship between Salmonella phage P22 and coliphage lambda, and discovered that bacteriophage genomes had a modular structure. (wikipedia.org)
Mutants3
Herskowitz also worked with Costa Georgopoulos on host genes that control DNA replication and host mutants with virus growth defects. (wikipedia.org)
Studies of heat-inducible lambda mutants. (wikidata.org)
Plasmid1
the mode of replication is a major factor in segregational plasmid instability in lactococcus lactis. (liverpool.ac.uk)
Genus2
A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. (lookformedical.com)
A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. (lookformedical.com)
Rect1
red/et recombination, a powerful homologous recombination system based on the red operon of lambda phage or rece/ rect from rac phage, provides an innovative approach for dna engineering. (liverpool.ac.uk)
A prototype of the small virulent DNAcoliphages, it is composed of a single strand of supercoiled circular DNA, which on infection, is converted to a double-stranded replicative form by a host enzyme. (lookformedical.com)
Scientific1
As a graduate student, Herskowitz made major contributions to the scientific understanding of regulation of gene expression in Lambda phage. (wikipedia.org)