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  • gene expression
  • DnaA is the bacterial replication initiator, which also functions as a transcription factor to regulate gene expression. (mit.edu)
  • Consequently, I can directly compare differences in gene expression due to the presence versus absence of dnaA. (mit.edu)
  • Deletion of dnaA results in approximately 463 significant differences in gene expression, most of which I show are due to DnaA direct activation of the gene sda. (mit.edu)
  • In addition to the many effects on gene expression, I show that deletion of dnaA results in lowered competence development. (mit.edu)
  • Characterization of the dnaA promoter revealed two cis -acting elements that have dramatic effects on dnaA gene expression. (asm.org)
  • The chromosomes of eukaryotic cells contain cis-acting elements important for gene expression, replication, folding and structure, segregation and recombination. (berkeley.edu)
  • This suggests that sequential changes in SeqA distribution orchestrate a program of gene expression that ensures coordinated DNA replication and cell division. (asm.org)
  • Exploiting a Nicotiana benthamiana pOri2 line, which is transformed with a transgene consisting of a direct repeat of the African cassava mosaic virus (ACMV)-replication origin (Ori) flanking a non-viral DNA region, and virus-induced RNA silencing (VIGS), the impact of host gene expression on replication of the ACMV-derived replicon was investigated. (springer.com)
  • subtilis
  • In B. subtilis, DnaA has previously been shown to repress its own transcription and has also been implicated in directing part of the transcriptional response to replication stress. (mit.edu)
  • For example, B. subtilis sporulation is disrupted in strains lacking tmRNA due to decreased expression of the SpoIVCA recombinase, an enzyme that is required for production of σ K during the developmental sigma factor cascade ( 1 ). (asm.org)
  • strains
  • I describe the global transcriptional changes in strains lacking Hda and suppressed by two different modes. (buffalo.edu)
  • A hitherto unknown function for either DnaA in replication restart or PriC in initiation of DNA replication that occurs in certain dnaA temperature sensitive mutant strains at the permissive temperature of 30°C has been documented. (biomedcentral.com)
  • Importantly, despite the overexpression of mprF and dlt , the relative net positive surface charge was decreased in the Dap r mutants, suggesting that other factors contribute to the surface charge alterations and that a simple charge repulsion mechanism could not entirely explain the Dap r phenotype in these strains. (asm.org)
  • locus
  • Isogenic dnaA+ cells were constructed similarly and have dnaA constitutively expressed from an ectopic locus. (mit.edu)
  • occurs
  • Elevated expression of the c-Myc transcription factor occurs frequently in human cancers and is associated with tumor aggression and poor clinical outcome. (mit.edu)
  • Replication stress occurs when key replication factors are limiting as a result of hypomorphic mutation or when cells in a quiescent niche are induced to enter a division cycle through defaults in cell proliferation controls. (berkeley.edu)
  • binds
  • The bacterial SeqA protein binds to hemi-methylated GATC sequences that arise in newly synthesized DNA upon passage of the replication machinery. (asm.org)
  • machinery
  • They need to reprogramme host cellular machinery for DNA replication. (springer.com)
  • These results suggest that the cellular machinery for DNA replication in differentiated tissues of older leaves cannot be reprogrammed by Rep alone but may need other uncharacterised viral and plant factors. (springer.com)
  • virulence factors
  • Pathogenicity depends on the acquisition of several virulence factors, of which the cholera toxin (CT) and the toxin-coregulated pilus (TCP) are considered the most significant. (prolekare.cz)