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  • vectors
  • Gene transfer using replication-defective human foamy virus vectors. (uzh.ch)
  • Replication-defective vectors based on an infectious molecular clone of human foamy virus (HFV) were constructed by deletion and replacement of the accessory genes with expression cassettes for puromycin-resistance and beta-glucouronidase. (uzh.ch)
  • Replication-incompetent lymphocytic choriomeningitis virus (rLCMV) vectors do not elicit vector-neutralizing antibody responses, and homologous prime-boost regimens with rLCMV vectors induce boostable and protective T cell responses to model antigens in mice. (jpt.com)
  • Here we investigated CD8 + T cell responses in the female mouse cervicovaginal mucosa after intravaginal immunization with human papillomavirus vectors (HPV pseudoviruses) that transiently expressed a model antigen, respiratory syncytial virus (RSV) M/M2, in cervicovaginal keratinocytes. (jci.org)
  • This way, if a viral production is required, the replicative elements must be provided by the host cell line (usually 293T) upon transfection with dedicated vectors bearing the packaging elements of the virus. (tebu-bio.com)
  • Cotransfection of 293T cells with these three plasmid components produced VSV-G pseudotyped, disabled, replication defective, bicistronic JDV vectors encoding the green fluorescent protein (EGFP) and the Neo resistance selection maker simultaneously with a titre range of (0.4-1.2) × 10 6 CFU/ml. (edu.au)
  • protein
  • So, we deleted that protein from the virus. (thenakedscientists.com)
  • So, what we did is we grew it on a cell line that provided the HSV-1 glycoprotein D. That just provides the protein on the outer surface of the viral envelope, allowing the virus to get in but once that virus is inside a cell it doesn't have the DNA to make more of that protein. (thenakedscientists.com)
  • H2AX phosphorylation has also been shown to be dispensable in other cases of DNA repair, such as in retroviral postintegration DNA rejoining or in replication protein A (RPA) focus formation after replication stress ( 16 , 33 ). (asm.org)
  • and re-inserting into said genome the deleted sequences within the defective genome wherein said sequences have a homology of at least 60% to the natural sequence of the virus, wherein said sequences code for an RNA-dependent RNA polymerase and at least one structural or non-structural protein, and wherein a fragment of said DNA is capable of being transcribed into RNA and assembled to a virion. (patentsencyclopedia.com)
  • vector
  • The design and construction of the JDV vector system should facilitate the study of the lentivirology and pathogenesis of the diseases associated with JDV or other bovine virus infections. (edu.au)
  • To our knowledge, this is the first such vector system developed from a cattle virus. (edu.au)
  • absence
  • Mice lacking B cells (microMT) were immunized, depleted of CD4 or CD8 T cells, and then challenged intravaginally with HSV-2 to elucidate T cell subset contributions in the absence of virus-specific antibody. (nih.gov)
  • Taken together, our studies demonstrate that, in the absence of serum, α-defensin-1 may act directly on the virus, but, in the presence of serum, its effects are on the cell, where it inhibits HIV-1 replication. (jci.org)
  • genes
  • Acquisition of oncogenicity by endogenous mouse type C viruses: effects of variations in env and gag genes. (asm.org)
  • bovine
  • Jembrana disease virus (JDV) is a newly isolated and characterised bovine lentivirus. (edu.au)
  • 83. The infective clone according to claim 80, wherein said full-length cDNA is flanked at the 3' end by a poly(A) tail, the ribozyme of the hepatitis delta virus (HDV), and the termination and polyadenylation sequences of bovine growth hormone (BGH). (patentsencyclopedia.com)
  • vaccination
  • Vaccination with vaccinia virus has been directly responsible for the successful eradication of smallpox (variola). (medscape.com)
  • protection
  • So, when we took mice and immunized them with this, and then challenged them with very high doses of wild type virus, we observed 100% complete protection. (thenakedscientists.com)
  • cell line
  • Cell lines which produced in excess of 10(5) helper virus-free transducing units/ml were generated by trans-complementation of the replication defect using a BHK-21-derived cell line expressing the Bel-1 transactivator. (uzh.ch)
  • Modulation of mouse mammary tumor virus production in the MJY-alpha cell line. (asm.org)
  • study
  • In this study, we used an inactivated adeno-associated virus (AAV) to induce a stalled replication fork signal and investigate the function of γH2AX. (asm.org)
  • role
  • The results establish a new role for H2AX in the p53/p21 pathway and indicate that H2AX is required for p21-induced cell cycle arrest after replication stalling. (asm.org)
  • once
  • Biodegradable Cationic Polymers (BCPs) and mimicks of virus nuclear targeting peptides (VIPs), once chemically attached to nucleic acids, can lead this exogenous material to passively cross the plasmic membrane. (tebu-bio.com)