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  • genome
  • The first of the components is a bicistronic transfer vector plasmid that was constructed to contain cis-sequences from the JDV genome, including 5′- and 3′-long terminal repeats (LTRs), 0.4 kb of truncated gag and 1.1 kb of 3′-env, a multiple cloning site to accommodate the gene(s) of interest for transfer, and an internal ribosome entry site plus the neomycin phosphotransferase (Neo) gene cassette for antibiotic selection. (edu.au)
  • Efficient co-expression of the five proteins was successful and titres obtained for these pentacistronic virus vectors (final genome size ∼7·9 kb) were comparable to those of vector systems with shorter genomes. (microbiologyresearch.org)
  • Other vectors constructed that exceeded the genome length of the wild-type virus suffered frequent deletions. (microbiologyresearch.org)
  • Many challenges remain, including understanding and preventing genotoxicity from integrating vectors or off-target genome editing, improving gene transfer or editing efficiency to levels necessary for treatment of many target diseases, preventing immune responses that limit in vivo administration of vectors or genome editing complexes, and overcoming manufacturing and regulatory hurdles. (nextbigfuture.com)
  • therapeutic
  • We also utilize replication-defective HIV as a vector to transduce non-dividing cells for gene therapeutic purposes and are developing novel methods of vector production. (yale.edu)
  • In vivo delivery of therapeutic AAV vectors to the retina, liver, and nervous system resulted in clinical improvement in patients with congenital blindness, hemophilia B, and spinal muscular atrophy, respectively. (nextbigfuture.com)
  • While some therapeutic substances can be delivered in free form, others require a carrier or vector in order to reach and enter their final destination, either due to their rapid clearance from the area of introduction or their inability to cross biological barriers, or due to their systemic toxicity. (allindianpatents.com)
  • Infection
  • Both used infection of the participants identified by seroconversion and/or detection of virus as the endpoint. (asm.org)
  • The world wide distribution of these two major vectors puts nearly a third of the global human population at risk of infection ( 2 ). (frontiersin.org)
  • Besides prior infection with one serotype, other factors influencing the disease outcome are the strain of infecting virus, age and gender, nutritional status, and the genetic background of the patient ( 11 - 16 ). (frontiersin.org)
  • Immunization
  • Here we investigated CD8 + T cell responses in the female mouse cervicovaginal mucosa after intravaginal immunization with human papillomavirus vectors (HPV pseudoviruses) that transiently expressed a model antigen, respiratory syncytial virus (RSV) M/M2, in cervicovaginal keratinocytes. (jci.org)
  • green fluores
  • Cotransfection of 293T cells with these three plasmid components produced VSV-G pseudotyped, disabled, replication defective, bicistronic JDV vectors encoding the green fluorescent protein (EGFP) and the Neo resistance selection maker simultaneously with a titre range of (0.4-1.2) × 10 6 CFU/ml. (edu.au)
  • Development
  • Our laboratory is focused on the study of human immunodeficiency virus type I (HIV) replication and the development of small animal models of HIV. (yale.edu)
  • A unique challenge in vaccine development against DENV is the high degree of sequence variation, characteristically associated with RNA viruses. (frontiersin.org)
  • high
  • High rates of frameshift mutations within homo-oligomeric runs during a single cycle of retroviral replication. (microbiologyresearch.org)
  • The developed virus neutralization assay is suitable for high-throughput testing and can be used for both animal studies and (large scale) vaccine clinical trials. (biomedcentral.com)
  • Ebola viruses represent a class of filoviruses that causes severe hemorrhagic fever with high mortality. (asm.org)
  • Ebola
  • A safe and effective Ebola virus vaccine is needed because of its continued emergence and its potential for use for biodefense. (asm.org)
  • Disease
  • Jembrana disease virus (JDV) is a newly isolated and characterised bovine lentivirus. (edu.au)
  • The picornavirus foot-and-mouth disease virus 2A sequence was combined with three different internal ribosome entry segments to construct and characterize three independent pentacistronic retroviruses of different sizes. (microbiologyresearch.org)
  • cell
  • Cell lines which produced in excess of 10(5) helper virus-free transducing units/ml were generated by trans-complementation of the replication defect using a BHK-21-derived cell line expressing the Bel-1 transactivator. (uzh.ch)