• Abstract Hepatic ischemia/reperfusion (I/R) injury is a common clinical challenge. (123dok.net)
  • The following review considers recent advances in the understanding of hepatic ischemia-reperfusion injury and focuses on inflammatory mediators of significance. (ectrx.org)
  • In the present study, the team was able to show that Liproxstatin-1 suppresses ferroptosis both in human kidney cells, Gpx4 deletion-induced acute renal failure and in a pre-clinical model of hepatic ischemia reperfusion injury. (sciencedaily.com)
  • In renal transplantation, delayed graft function and primary nonfunction are major sources of graft loss and morbidity. (ucl.ac.uk)
  • Since the shortage of donor organs is a major problem, it is imperative to develop methods capable of preserving organs after significant periods of warm and cold ischaemia, and at the same time minimise the reperfusion injury associated with transplantation. (ucl.ac.uk)
  • One of the primary causes of ARF is I/R which is a drop in blood flow leading to inadequate supply of oxygen and nutrients to renal tissue which can be caused by, amongst others, surgery, organ transplantation and shock [ 1 ]. (biomedcentral.com)
  • The injury an organ sustains during recovery, preservation, and transplantation occurs primarily as a result of ischemia and hypothermia. (medscape.com)
  • Damage to organs during transplantation occurs in 2 phases: the warm ischemic phase and the cold ischemic phase. (medscape.com)
  • In liver transplantation, tissue damage at reperfusion is mostly correlated with warm and cold ischemia times and leads in turn to poor graft function [13] and biliary complications [14,15]. (123dok.net)
  • Thrombotic microangiopathy is not a rare complication of kidney transplantation and is characterized by microangiopathic hemolytic anemia, thrombocytopenia, and acute kidney injury with extensive thrombosis of the arterioles and capillaries. (ekjt.org)
  • Various factors can cause thrombotic microangiopathy after kidney transplantation, including surgery, warm and cold ischemia-reperfusion injury, exposure to immunosuppressants, infection, and rejection. (ekjt.org)
  • With the rise in the population of patients with End-stage Renal Disease (ESRD) in Nigeria, there is an increased demand for Renal Replacement Therapy (RRT) including kidney transplantation (KT). (lidsen.com)
  • In transplantation medicine, special preservation solutions with often unphysiological, i.e. intracellular ion compositions are used for tissue and organ preservation during extracorporal cold storage, for example University of Wisconsin (UW) solution [ 19 ]. (biomedcentral.com)
  • Donor hearts were stored in cold histidine-tryptophan- α -ketoglutarate-N solution for 12 h and underwent heterotopic, isogenic transplantation, except 5 hearts of each group, which were analysed immediately after preservation. (frontierspartnerships.org)
  • Chronic allograft injury (CAI), consisting of vasculopathy and interstitial fibrosis, affects approximately 50% of patients after 10 years and limits long-term survival following heart transplantation ( 1 ). (frontierspartnerships.org)
  • Reperfusion injury significantly impacts clinical outcome after pulmonary transplantation. (jamanetwork.com)
  • Ischemia-reperfusion injury after lung transplantation increases risk of late bronchiolitis obliterans syndrome. (jamanetwork.com)
  • Influence of graft ischemia time on outcomes following lung transplantation. (jamanetwork.com)
  • Pulmonary transplantation: the role of brain death in donor lung injury. (jamanetwork.com)
  • However, the studies in this thesis illustrate that various donor, donor management, and organ preservation related factors have a profound impact on outcome after renal transplantation. (5dok.net)
  • The American Organ Procurement and Transplantation Network (OPTN) database can be utilized to obtain additional detailed information on the association beween cold ischemic time and posttransplant outcome. (5dok.net)
  • When the cohort of deceased donor single kidney recipients between 1994 and 2007 (n=99,860) is studied, all important end points after transplantation, including acute rejection and graft survival, are strongly influenced by a few hours rise in cold ischemic time above 8 hours (Figure 1a-e). (5dok.net)
  • The remaining 10% of cases are performed as pancreas transplantation alone in patients who have normal renal function, but with very labile and problematic diabetes, such as patients with life-threatening hypoglycemic unawareness. (medscape.com)
  • Studies comparing renal function in SPK transplantation recipients versus diabetic KTA recipients did not demonstrate significant differences during the early posttransplant period. (medscape.com)
  • Neuropathy improves after both kidney and pancreas transplantation, suggesting that renal failure and diabetes contribute to the sensory neuropathy commonly observed at the time of transplantation. (medscape.com)
  • 5-8 The incidence of PVT is 1%-3%, which can lead to mesenteric ischaemia or even a negative impact on graft function and transplantation outcomes. (rcpjournals.org)
  • I/R injury, through the liberation of radical oxygen species and the activation of inflammatory pathways, induces cellular injury and mi-crocirculatory damage, which translate to organ dysfunc-tion, morbidity, and increased health care costs [7, 8]. (123dok.net)
  • Additionally, machine reperfusion induces the production of ROS, which results in I/R injury to the heart. (gotomydoctor.com)
  • One of the primary causes of ARF is ischemia/reperfusion (I/R). Inflammatory process and oxidative stress are thought to be the major mechanisms causing I/R. MK-886 is a potent inhibitor of leukotrienes biosynthesis which may have anti-inflammatory and antioxidant effects through inhibition of polymorphonuclear leukocytes (PMNs) infiltration into renal tissues. (biomedcentral.com)
  • Ovechkin AV, Lominadze D, Sedoris KC, Robinson TW, Tyagi SC, Roberts AM. Lung ischemia-reperfusion injury: implications of oxidative stress and platelet-arteriolar wall interactions. (jamanetwork.com)
  • Numerous preclinical models have depicted the impact of I/R injury and hepatic parenchymal quality (steatosis, age) on increased cancer growth in the injured liver. (123dok.net)
  • Furthermore, we reviewed the current treatments available for neonates who have undergone intrapartum asphyxia, and highlight the emergence of maternal dietary creatine supplementation as a preventative therapy, which has been shown to provide multi-organ protection from birth asphyxia-induced injury in our preclinical studies. (frontiersin.org)
  • The multivariate models in chapters 3 and 5 show that increased cold ischemic time is significantly and independently associated with an elevated risk of delayed graft function and graft failure. (5dok.net)
  • Allograft renal artery thrombosis, venous thrombosis, and arterial pseudo-aneurysms were encountered in 3, 2 and 4 recipients respectively. (lidsen.com)
  • Liver ischemia and reperfusion - which cause liver damage that is significant in a variety of diseases, injuries, and procedures (including but not limited to trauma and transplant) - have been the focus of many investigations in recent years. (ectrx.org)
  • Ischemia-reperfusion injury, which involves complex inflammatory pathways, is a significant cause of liver damage and an important factor in a variety of diseases, injuries, and procedures that include (but not limited to) transplant and trauma. (ectrx.org)
  • Nitric oxide production (by iNOS) may play several roles in renal pathophysiology, including induction of tubular damage. (biomedcentral.com)
  • RESULTS: A decrease in renal function was shown after 2 hours of cold ischemia with tubular necrosis and mild cell infiltration, while after 24 hours of cold ischemia there was severe renal failure with tubular and glomerular necrosis, and leukocyte infiltration. (unica.it)
  • More specifically, the deletion of the ferroptosis-regulating enzyme Gpx4 in a pre-clinical model results in high ferroptosis rates in kidney tubular epithelial cells causing acute renal failure. (sciencedaily.com)
  • The results show that, following induction of HO-1 in renal tissue, the enzymatic activity was maintained during hypothermia, and some beneficial effects on renal function could be observed after storage and reperfusion. (ucl.ac.uk)
  • These are placed into insulin-dependent diabetics with end-stage renal disease (ESRD). (medscape.com)
  • Acute renal failure (ARF) is an important clinical problem with a high mortality and morbidity. (biomedcentral.com)
  • Acute kidney injury (AKI) is a common clinical syndrome characterized by rapid deterioration of renal function. (biomedcentral.com)
  • Although limited, there is growing clinical evidence that I/R injury and liver quality are associated with the risk of post-sur-gical cancer recurrence. (123dok.net)
  • There is significant clinical overlap between secondary TMA and atypical hemolytic uremic syndrome (aHUS) in renal transplant patients due to the diverse triggers of TMA in this environment, which can occur alongside various genetic predispositions. (ekjt.org)
  • Left ODN was performed in 152 (72.4%) of cases and the commonest indication for right ODN was multiple left renal arteries. (lidsen.com)
  • The major drawback of such strategies is that they can induce I/R injury to the residual liver parenchyma [4, 12]. (123dok.net)
  • This review aims at giving the last updates regarding the role of hepatic I/R and liver parenchymal quality injury in the setting of oncological liver surgery, using a ''bench-to-bedside'' approach. (123dok.net)
  • Putative pathophysiological mechanisms linking I/R injury and liver cancer recurrence include an increased implantation of circulating cancer cells in the ischemic liver and the upregulation of proliferation and angiogenic factors following the ischemic insult. (123dok.net)
  • the liver, I/R injury is at the source of poor outcomes after surgical procedures such as hepatectomy and liver trans-plantation [7]. (123dok.net)
  • Investigations involving animal models and human trials are reviewed, a summary of significant studies in human subjects is presented ( Table 1 ), and the pathways and mediators important in liver ischemia-reperfusion injury are described ( Figure 1 ). (ectrx.org)
  • The peroxidation of lipids (16, 17), protein oxidation, and the formation of peroxinitrites frequently occur after liver ischemia and reperfusion. (ectrx.org)
  • Liver ischemia-reperfusion injury is ameliorated by treatment with various free radical scavengers, as shown in investigations of allopurinol, superoxide dismutase, catalase, and other antioxidant compounds. (ectrx.org)
  • Recent evidence has shown that in cases of generalized ischemia after hemorrhagic shock, the effectiveness of melatonin in improving liver function depends on the melatonin receptor (40). (ectrx.org)
  • This herb helps boost the immune system by warming, stimulating and harmonizing the kidney, liver, spleen and stomach. (trilliumhealthsolutions.com)
  • Before cold ischemia all harvested kidneys were subjected to 30 to 45 minutes of warm ischemia. (unica.it)
  • Several physical symptoms include shrinkage of one or both kidneys, renovascular hypertension, acute renal failure, progressive azotemia, and acute pulmonary edema. (wikipedia.org)
  • Deceased donor kidneys should always be transplanted as soon as possible, for a few hours reduction in cold ischemic time will result in a significant and clinically relevant improvement in outcome. (5dok.net)
  • Ischemia causes not only insufficiency of oxygen, but also reduced availability of nutrients and inadequate removal of metabolic wastes. (wikipedia.org)
  • i.e. concentrations as in RPMI 1640) evoked a cell injury and loss of metabolic function corresponding to that observed in RPMI 1640. (biomedcentral.com)
  • Ischemia-reperfusion injury following the relieving of muscle compression may cause metabolic changes and rhabdomyolysis depending on the time of extrication. (turkjemergmed.com)
  • Researchers have applied the idea of metabolic reduction to the cold storage of hearts. (gotomydoctor.com)
  • The allograft was placed in the right iliac fossa in 187 (89.0%) recipients with the external iliac artery preferred for anastomosis with the allograft renal artery in end-to-side fashion in 182 (86.7%) cases. (lidsen.com)
  • Paralysis is a very late sign of acute arterial ischemia and signals the death of nerves supplying the extremity. (wikipedia.org)
  • Ischemia is a vascular disease involving an interruption in the arterial blood supply to a tissue, organ, or extremity that, if untreated, can lead to tissue death. (wikipedia.org)
  • Venous problems like venous outflow obstruction and low-flow states can cause acute arterial ischemia. (wikipedia.org)
  • An aneurysm is one of the most frequent causes of acute arterial ischemia. (wikipedia.org)
  • 3) to search for novel "pharmacological preconditioning agents" that are capable of selectively activating the HO-1 gene in renal tissues. (ucl.ac.uk)
  • The Chi-square test was used to compare the occurrence of postoperative complications and postoperative renal function. (biomedcentral.com)
  • Complications following LT occur commonly and may derive from pretransplant recipient condition, graft-specific factors (including preservation and ischemia-reperfusion injury), technical or intraoperative complications, the immunologic response to the graft, or infection. (musculoskeletalkey.com)
  • Although the mechanisms of ischemia-reperfusion injury are numerous and complex, many advances in treatment have been made. (ectrx.org)
  • Ischemia or ischaemia is a restriction in blood supply to any tissue, muscle group, or organ of the body, causing a shortage of oxygen that is needed for cellular metabolism (to keep tissue alive). (wikipedia.org)
  • PubMed was searched using general terms including rhabdomyolysis, crush injury, and earthquake in English language without time restriction. (turkjemergmed.com)
  • Chronic ischemia of the brain may result in a form of dementia called vascular dementia. (wikipedia.org)
  • We assessed whether donor preservation with S-NO-HSA affects isograft injury and myocardial expression of GATA2 as well as miR-126-3p, which are considered protective against vascular and endothelial injury. (frontierspartnerships.org)
  • In addition to ischaemic tissue damage, seen for example in renal failure, stroke or cardiovascular diseases, the mechanism may also play a central role in neurodegenerative diseases and in comorbidities of diabetes mellitus. (sciencedaily.com)
  • Failure to treat could cause chronic kidney disease and a need for renal surgery. (wikipedia.org)
  • The protective effects of antileukocyte function associated antigen 1 mAb on renal injury was not as dramatic as in rodent models but a significant improvement in renal function was observed in treated animals after 2 hours of cold ischemia. (unica.it)
  • In this discussion of antioxidant molecules, it is also important to include melatonin, which exerts multiple protective effects in hepatic ischemia and reperfusion (33-36). (ectrx.org)
  • The goal of this proposal is to investigate a novel preservation method utilizing hydrogen sulfide (H2S) to induce a protective state of hibernation against I/R injury. (gotomydoctor.com)
  • Similar Ca 2+ and phosphate concentrations did not increase cold-induced cell injury in the kidney cell line LLC-PK 1 , porcine aortic endothelial cells or rat hepatocytes. (biomedcentral.com)
  • However, more extreme conditions (Ca 2+ was nominally absent and phosphate concentration raised to 25 mM as in the organ preservation solution University of Wisconsin solution) also increased cold-induced injury in rat hepatocytes and porcine aortic endothelial cells. (biomedcentral.com)
  • PURPOSE: We established a primate model to investigate the effects of the antileukocyte function associated antigen 1 (CD 11a) mAb odulimomab (Imtix-Sangstad, Lyon, France) for preventing renal ischemia-reperfusion injury. (unica.it)
  • Groups 1 and 3 monkeys were treated with an antileukocyte function associated antigen 1 mAb before cold ischemia and then for 3 days, while groups 2 and 4 monkeys received an IgG1 isotype control. (unica.it)
  • In all groups renal function was investigated before warm ischemia and 72 hours after reperfusion. (unica.it)
  • A significant improvement in renal function and decrease in kidney lactoferrin content was evident in group 1 compared to group 2 at 72 hours, while no significant difference was noted in groups 3 and 4. (unica.it)
  • In conclusion, on top of its harmful early impact on organ function, I/R injury is linked to increased tumor growth. (123dok.net)
  • Therapeutic strategies tackling I/R injury could not only improve post-surgical organ function, but also allow a reduction in the risk of cancer recurrence. (123dok.net)
  • The ECD-derived kidney was worse than the SCD-derived kidney in terms of graft survival and AR occurrence, and trend to an inferior renal function postoperative. (biomedcentral.com)
  • I am currently funded by NIEHS to assess the role of sirtuin 3 in regulating PCB-induced mitochondrial injury through AREA Grant mechanism, which is instrumental for exposing students to research. (uams.edu)
  • The process of ischemia and reperfusion is known to cause inducible nitric oxide synthase induction and activation, and there is evidence that interleukin (IL)-1β (46), IL-1 receptor (47), and IL-1 receptor along with nuclear factor-kappa beta (48) may have an important role in that induction. (ectrx.org)
  • The signs and symptoms of ischemia vary, as they can occur anywhere in the body and depend on the degree to which blood flow is interrupted. (wikipedia.org)
  • In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. (biomedcentral.com)
  • this is termed donor warm ischemic time (WIT). (medscape.com)
  • Apart from the interventions that are studied in this thesis, one very important factor is cold ischemic time. (5dok.net)
  • These figures show that the usual assumption that a deceased donor kidney is safe as long as cold ischemic time stays below 18-24 hours is incorrect. (5dok.net)
  • of the average cold ischemic time with just a few hours is likely to result in an improved postttansplant outcome which is in the same order as the effect that low-dose ciclosporin + daclizumab had versus standard-dose ciclosporin. (5dok.net)
  • Although the majority of research is directed toward reducing the brain injury that results from intrapartum birth asphyxia, the multi-organ injury observed in surviving neonates is of equal importance. (frontiersin.org)
  • I am mostly interested in apoptotic endonucleases, the key enzymes regulating irreversible cell death after cell injury and during diseases. (uams.edu)
  • Moringa is a genus of medicinal plants that has been used traditionally to cure wounds and various diseases such as colds and diabetes. (frontiersin.org)
  • MATERIALS AND METHODS: We randomly divided 34 Macaca cynomolgus monkeys into groups 1 and 2, which received a renal autograft after 2 hours of cold ischemia, and groups 3 and 4, which received the autograft after 24 hours of cold ischemia. (unica.it)
  • Current methods of cold static storage have reached their limits in storage time due to the extent of ischemia-reperfusion (I/R) injury induced during static cold storage. (gotomydoctor.com)
  • The extent of reperfusion injury is directly proportional to preservation time in cold storage, and research has shown that with static storage methods, heart storage time will not exceed six hours. (gotomydoctor.com)
  • For example, patients with myocardial ischemia have a decreased blood flow to the heart and are prescribed with medications that reduce chronotrophy and ionotrophy to meet the new level of blood delivery supplied by the stenosed vasculature so that it is adequate. (wikipedia.org)
  • There was no observed difference in survival rates when grafts were exposed to 6 or more hours of ischemia ( P = .41, log-rank test). (jamanetwork.com)
  • We hypothesize that a continuous release of H2S in the preservation solution will result in better protection of the heart during cold storage. (gotomydoctor.com)
  • Past studies have experimented with either one-time injection of NaHS solution or 30-minute incubation with H2S before ischemia. (gotomydoctor.com)
  • A sudden, brief episode (symptoms lasting only minutes) of ischemia affecting the brain is called a transient ischemic attack (TIA), often called a mini-stroke. (wikipedia.org)
  • The objective of present study was to assess the effects of MK-886 and DITPA on renal I/R injury. (biomedcentral.com)
  • Some effects of H2S protection require the continued presence of H2S, such as ROS scavenging during ischemia. (gotomydoctor.com)
  • In addition to this iron-dependent pathway, other changes in cellular ion homeostasis have also been described to contribute to cold-induced cell injury. (biomedcentral.com)
  • Classically, a cellular accumulation of sodium due to a reduced Na + /K + -ATPase activity resulting in cell swelling was thought to cause cold-induced cell injury [ 19 , 20 ]. (biomedcentral.com)
  • In the oncological setting, there are growing concerns regarding the deleterious impact of I/R injury on the risk of post-surgical tumor recurrence. (123dok.net)