The Tudor domain binds to proteins with dimethylated arginine or lysine residues, and may also bind methylated histone tails to facilitate protein-protein interactions. (nih.gov)
All the anhydrate-modified globulin-like proteins showed potent anti-HIV activity, which is correlated with the percentage of modified lysine and arginine residues in the modified protein. (biomedcentral.com)
Protein arginine methyltransferases (PRMTs) comprise a group of enzymes that catalyze the transfer of methyl groups to the amino acid arginine or the arginine residues of proteins in eukaryotic species. (e-enm.org)
While all three types of PRMTs are capable of catalyzing monomethylation of arginine residues, type I PRMTs can transfer a second methyl group to the monomethylated nitrogen atom of the guanidino group of monomethyl arginine, resulting in the formation of proteins containing one or more asymmetric dimethylarginine residues. (e-enm.org)
Type II PRMTs transfer a methyl group to the unmodified nitrogen atom of the guanidine group of monomethyl arginine to form proteins with symmetric dimethylarginine residues. (e-enm.org)
This process is used for labeling or purification of proteins by removing the N-terminal residue of peptides without damaging the whole structure of the protein. (psiberg.com)
27. Rämisch S, Weiniger U, Martinsson J, Akke M and Andre I "Computational design of Leucine-Rich Repeat proteins with a defined geometry" Proc Natl Acad Sci, 2014, pii: 201413638. (lu.se)
Histones3
In earlier reports, PRMTs promoted active transcription of target genes by mediating asymmetric dimethylation of arginine residues on histones, resulting in increased acetylation and the subsequent activation of transcription. (e-enm.org)
Unlike with trypsin, LysargiNase cleavage also occurs at methylated lysine and arginine, allowing detection of these epigenetic modifications. (sigmaaldrich.com)
It is resistant to trypsinhydrolysis, possibly because the lysine and arginine residues in OVA are modified by ML. Mechanism studies suggest that ML-OVA inhibits HIV-1 entry by targeting gp120 on HIV-1 virions and also the CD4 receptor on the host cells. (biomedcentral.com)
This cleavage results in peptides with molecular weights similar to tryptic peptides, but with N-terminal lysine or arginine residues that can be fragmented with b ion-dominated spectra. (sigmaaldrich.com)
5. Step 4 is repeated using different cleavage procedures to generate different and overlapping segments of peptides. (flashcardmachine.com)
A similar distribution of amino acid residues is typical for linear (without disulfide bonds) cationic cytolytic peptides from the venoms of other snakes and insects. (actanaturae.ru)
Amino acids5
The amino acid sequence of one representative TALE repeat is shown on top, with RVD amino acids 12 and 13 marked with a grey box and KQ diresidue at positions 16 and 17 involved in DNA phosphate binding in bold and underlined. (nature.com)
Ankyrin repeats are about 33 amino acids long and occur in at least four consecutive copies. (embl.de)
Ankyrin repeats are tandemly repeated modules of about 33 amino acids. (embl.de)
The amphiphilicity is caused by positively charged lysine and arginine residues flanking the tips of the loops that consist primarily of hydrophobic amino acids. (actanaturae.ru)
TDRD7, also called PCTAIRE2-binding protein, or Tudor repeat associator with PCTAIRE-2 (Trap), is a component of specific cytoplasmic RNA granules involved in post-transcriptional regulation of specific genes: probably acts by binding to specific mRNAs and regulating their translation. (nih.gov)
This can improve protein C-terminal peptide identification and several arginine rich phosphosite assignments. (sigmaaldrich.com)
Protein arginine methyltransferases (PRMTs) were recently added to the list of enzymes that are critical for regulating transcription in hepatic gluconeogenesis. (e-enm.org)
3)High purity: Higher specific activity.Host protein residues is less than the limits of biological products. (recombinanttrypsin.com)
The ankyrin repeat is one of the most common protein-protein interaction motifs in nature. (embl.de)
Crystal structure of the ARF-GAP domain and ankyrin repeats of PYK2-associated protein beta. (embl.de)
PAPbeta, a protein that binds to and is phosphorylated by the non-receptor tyrosine kinase PYK2, contains several modular signaling domains including a pleckstrin homology domain, an SH3 domain, ankyrin repeats and an ARF-GAP domain. (embl.de)
BACKGROUND The histone methyltransferase (HMT) family includes histone lysine methyltransferases (HKMTs) and histone/protein arginine methyltransferases (PRMTs). (cancerindex.org)
Substitution4
With HypoPP hyperpolarization-activated cation leak counteracting K + -rectifying current, mutations cause outermost arginine or lysine substitution. (medscape.com)
The sequence analyses of the viruses showed that the HA cleavage site contained SPQRE K RRKKR, which differed from the 2004 H5N1 virus by an arginine-to-lysine substitution at position 341. (cdc.gov)
An AR construct with the charged lysine substitution by arginine (AR-618R) reduces RNA Pol II binding, AR transcriptional activity, prostate cancer cell growth, and xenograft tumor formation due to attenuation of AR nuclear translocation, whereas, construct mimicking neutral polar substitution acetylation at K618 by glutamine (AR-618Q) enhanced these effects beyond that of the wild-type AR. (oncotarget.com)
Positively2
In potassium and sodium channels, voltage-sensing S4 helices contain positively-charged lysine or arginine residues in repeated motifs. (wikipedia.org)
Upon depolarization, the positively-charged residues on the S4 domains move toward the exoplasmic surface of the membrane. (wikipedia.org)
Hydrophobic1
An invariant arginine and several nearby hydrophobic residues are solvent exposed and are predicted to be the site of interaction with ARFs. (embl.de)
Helices2
The structure contains 12 transmembrane helices, with the first 10 consisting of an inverted structural repeat of 5 transmembrane helices like LeuT (TC #2.A.22.4.2). (tcdb.org)
Each repeat folds into a helix-loop-helix structure with a beta-hairpin/loop region projecting out from the helices at a 90 o angle. (embl.de)
The first amino acid residue is released from the rest of the peptide chain by cleavage as ATZ amino acid. (psiberg.com)
Successful coupling of peptide epitopes to beads followed three steps: 1) development of a peptide tag appended to the C-terminus of each peptide epitope consisting of beta-alanine-lysine (x 4)--cysteine, 2) bead modification with a high concentration of adipic acid dihydrazide, and 3) use of the peptide epitope as a blocker in place of the traditional choice, bovine serum albumin (BSA). (cdc.gov)
Acetylation1
However, the ARD1-targeted residue within AR and the mechanisms of the acetylation event in prostate tumorigenesis remained unknown. (oncotarget.com)
Sequences1
The sequences are about 300 base pairs long and are repeated several thousand times throughout the genome. (ojp.gov)
Reaction1
However, at an inflammatory focus, arginine residues in LL-37 can be converted to citrulline via a reaction catalyzed by peptidyl-arginine deiminases (PAD2 and PAD4), which are expressed in neutrophils and are highly active during the formation of neutrophil extracellular traps (NETs). (cdc.gov)
Form2
Indeed, given that four guanine residues can self-associate in a planar arrangement through a Hoogsteen-type hydrogen-bonding network [ 4 ] to form a structure called G-quartet, G-rich strands can adopt a peculiar three-dimensional arrangement called G-quadruplex DNA [ 5 - 9 ] resulting from the stacking of several contiguous G-quartets (Figure 1 ). (hindawi.com)
Four ankyrin repeats are also present, the first two of which form an extensive interface with the ARF-GAP domain. (embl.de)
Activity3
It is no activity loss after 10 times repeated freezing and thawing. (recombinanttrypsin.com)
Site-directed mutagenesis of these residues confirms their importance in ARF-GAP activity. (embl.de)
Citrullination-Resistant LL-37 Is a Potent Antimicrobial Agent in the Inflammatory Environment High in Arginine Deiminase Activity. (cdc.gov)
Occur2
As muscle is converted to meat, the muscle fibers modify their metabolism in order to adapt to the new conditions (absence of oxygen and nutrient supply, lack of residues removing), and as a consequence, many changes occur. (earthwormexpress.com)
Contacts occur in discontinuous patches, suggesting a combinatorial quality for ankyrin repeat specificity. (embl.de)
Alpha1
The first two repeats cover an alpha helically ordered segment containing the p65 nuclear localization signal. (embl.de)
Crystal2
The structure and function of the cadaverine-lysine antiporter, CadB (2.A.3.2.2), and the putrescine-ornithine antiporter, PotE (2.A.3.2.1), in E. coli have been evaluated using model structures based on the crystal structure of AdiC (2.A.3.2.5), an agmatine-arginine antiporter. (tcdb.org)
The crystal structure of the PAPbeta ARF-GAP domain and the C-terminal ankyrin repeats has been determined at 2.1 A resolution. (embl.de)
More detailed structure-function analysis revealed a distinct subclass of GATA4/NKX2-5 inhibitory compounds with an acetyl lysine-like domain that contributed to ventricular cells (%Myl2-eGFP+). (biomedcentral.com)
The structure of the IkappaBalpha ankyrin repeat domain, bound to a partially truncated NF-kappaB heterodimer (p50/ p65), has been determined by X-ray crystallography at 2.7 A resolution. (embl.de)
Structures1
Myofibrils are cylindrical structures made up of repeated units known as sarcomeres that cause the striated appearance of muscle when meat is observed by electron microscopy. (earthwormexpress.com)
Shown2
TALE repeats with selectivity for canonical nucleobases are shown on the right with RVDs specified. (nature.com)
Eight regular repeats of the CRD (dark grey, numbering 1-8) and repeats of the NTR (light grey) are shown. (nature.com)
Current1
It is thought that the first 4 arginines account for the gating current, moving toward the extracellular solvent upon channel activation in response to membrane depolarization. (wikipedia.org)
Domains1
It shows a stack of six IkappaBalpha ankyrin repeats facing the C-terminal domains of the NF-kappaB Rel homology regions. (embl.de)
Core1
The core of the repeat seems to be an helix-loop-helix structure. (embl.de)
Analysis2
anticoagulation was accomplished with ethylenediaminetetraacetic acid (EDTA) for repeated biochemistry analysis and complete blood count. (cdc.gov)
Integrated Analysis of Genetic Abnormalities of the Histone Lysine Methyltransferases in Prostate Cancer. (cancerindex.org)
Short2
For example, one copy of a specific short tandem repeat (STR) region might have 10 repeats, while the other copy might have 11 repeats. (ojp.gov)
In 2011 and 2013 novel methods of genome editing appeared - this are TALEN (Transcription Activator-Like Effector Nucleases) and CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems. (actanaturae.ru)