OxidativeEpithelialApoptosisInhibitionAttenuateTubularCerebral ischemiaSerumFerroptosisCytokinesIschemic reperfusionSignaling pathwayBilateral renalEvaluation of renalTissuesRats were subjectedDamage inducedMiceCardiacAcute kidneyInjury inducedInducePost renalMitochondrialInflammatory2022Tissue damageShamCaspaseCardiovascularHemodynamicallyPhysiology1996HypoxiaErythropoietinProteinTherapeutic approachesCellTransplantationFunctionSignificantlyModelAssess
Oxidative4
- One of the primary causes of ARF is ischemia/reperfusion (I/R). Inflammatory process and oxidative stress are thought to be the major mechanisms causing I/R. MK-886 is a potent inhibitor of leukotrienes biosynthesis which may have anti-inflammatory and antioxidant effects through inhibition of polymorphonuclear leukocytes (PMNs) infiltration into renal tissues. (biomedcentral.com)
- The mechanism of cisplatin-induced acute kidney injury includes oxidative stress, mitochondrial dysfunction, and endoplasmic reticulum stress, which result in apoptosis, necrosis and ferroptosis of renal tubular epithelial cells, causing rapid loss of kidney function [ 8 - 11 ]. (ijbs.com)
- Protective effect of aqueous saffron extract (Crocus sativus L.) and crocin, its active constituent, on renal ischemia-reperfusion-induced oxidative damage in rats. (sciendo.com)
- These supplements can help to ameliorate oxidative stress, inflammation, proteinuria and progressive renal damage. (drprincetta.com)
Epithelial7
- Membrane attack complexes (MACs) formed following complement activation ultimately injure the kidney due to epithelial renal tubular cell apoptosis. (nature.com)
- Apoptotic epithelial tubular cells release pro-fibrinogenic factors, which, combined with complement-mediated inflammatory cell recruitment, promote fibrosis. (nature.com)
- Acts upstream of with a negative effect on epithelial cell apoptotic process. (nih.gov)
- More specifically, the deletion of the ferroptosis-regulating enzyme Gpx4 in a pre-clinical model results in high ferroptosis rates in kidney tubular epithelial cells causing acute renal failure. (sciencedaily.com)
- Thus, the present study elucidated a novel mechanism by which both BNIP3-mediated and PINK1-PARK2-mediated mitophagy protects against cisplatin-induced renal tubular epithelial cell ferroptosis through the ROS/HO1/GPX4 axis. (ijbs.com)
- In this study, we induced iPSCs differentiation into RPCs using a nephrogenic cocktail of factors combined with the renal epithelial cell growth medium. (biomedcentral.com)
- We showed that the differentiation efficiency of iPSCs to RPCs increased when cultured with renal epithelial cell growth medium after stimulation with a nephrogenic cocktail of factors. (biomedcentral.com)
Apoptosis4
- nuclear aspect-κB (NF-κB) which sets off the discharge of some inflammatory mediators such as for example tumor necrosis aspect-α (TNF-α) interleukin (IL)-1β and IL-61 6 On the tissues level ROS and inflammatory cytokines activate enzymes which are mixed AKT inhibitor VIII manufacture up in procedures of necrosis and apoptosis the last mentioned of which is normally most significant in reperfusion damage7. (liveconscience.com)
- It does not have the morphological characteristics of typical necrosis, such as swelling of the cytoplasm and organelles and rupture of the cell membrane, nor does it have the characteristics of traditional cell apoptosis, such as cell shrinkage, chromatin condensation, formation of apoptotic bodies and disintegration of the cytoskeleton. (nature.com)
- The transplantation of iPSC-derived RPCs decreased the levels of biomarkers indicative of renal injury and attenuated the necrosis and apoptosis of renal tissues, but resulted in the up-regulation of renal tubules formation, cell proliferation, and the expression of pro-renal factors. (biomedcentral.com)
- Our results revealed that iPSC-derived RPCs can protect AKI rat from renal function impairment and severe tubular injury by up-regulating the renal tubules formation, promoting cell proliferation, reducing apoptosis, and regulating the microenvironment in the injured kidney. (biomedcentral.com)
Inhibition6
- Therefore, in the present study, we evaluated the effect of mitochondrial complex 1 inhibition by rotenone on the chronic renal damage induced by acute ischemia-reperfusion. (oncotarget.com)
- Based on conflicting evidence of apoptotic induction and anti-inflammation of CK2 inhibition we assessed the effects of TBBt within the intrinsic response to renal I/R injury. (liveconscience.com)
- CK2α inhibition attenuates renal I/R injury To assess the function of CK2α in renal I/R injury we pretreated mice with TBBt a CK2α inhibitor 3 and 24?h prior to inducing I/R injury and blood samples were collected 24?h after reperfusion. (liveconscience.com)
- Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition. (immunochemistry.com)
- Inhibition of Caspase-1 Activation in Endothelial Cells Improves Angiogenesis: A NOVEL THERAPEUTIC POTENTIAL FOR ISCHEMIA. (immunochemistry.com)
- Upregulation of miR‑133b‑5p contributes to HPC‑mediated cardioprotection in cardiomyocytes, and the mechanism may be associated with inhibition of caspase‑8 and caspase‑3 apoptotic signaling. (spandidos-publications.com)
Attenuate2
- Adipose-derived SF-MSCs attenuate CIN by exerting anti-apoptotic effects, indicating that SF-MSCs may be a potential therapy for CIN. (asn-online.org)
- has been demonstrated to attenuate renal ischemia‑reperfusion (I/R) injury. (spandidos-publications.com)
Tubular4
- Danger-associated molecular patterns (DAMPs) are endogenous ligands released from renal tubular cells following ischemia/reperfusion injury that activate the complement system through the classical, lectin and alternative pathways. (nature.com)
- Nitric oxide production (by iNOS) may play several roles in renal pathophysiology, including induction of tubular damage. (biomedcentral.com)
- Bone marrow mesenchymal stem cell-derived exosomal miR-30e-5p ameliorates high-glucose induced renal proximal tubular cell pyroptosis by inhibiting ELAVL1. (immunochemistry.com)
- Acute kidney injury (AKI) is characterized by acute tubular injury and a rapid decline in renal function [ 1 ]. (biomedcentral.com)
Cerebral ischemia3
- The present study was designed to investigate the expression changes of PPAR-alpha, -beta, -gamma and NF-kappa B in the hippocampus of rats with global cerebral ischemia/reperfusion injury (GCIRI) after treatment with agonists or antagonists of PPAR-gamma. (biomedcentral.com)
- Global cerebral ischemia/reperfusion injury (GCIRI) occurs in patients who are successfully resuscitated from various clinical conditions such as cardiac arrest, asphyxia and shock, which are frequently accompanied by inflammation and can lead to serious neuronal injury, and further to neurodegeneration and learning and memory impairment [ 12 ]. (biomedcentral.com)
- Proinflammatory cytokines, such as interleukin (IL)-1β, IL-6 and tissue necrosis factor (TNF)-α, have been implicated as important mediators of injury following cerebral ischemia [ 13 ] and contribute to pathogenesis, exacerbating brain tissue damage following ischemia/reperfusion (I/R) injury [ 14 ]. (biomedcentral.com)
Serum7
- At 24 h post-operatively, we assessed the serum levels of creatinine and urea nitrogen, expression levels of peroxisome proliferator-activated receptor gamma (PPAR-γ) and NF-κB-related (p-IKK-β and IκB-α) proteins, and mRNA expression levels of the inflammatory cytokines, including TNF-α and MCP-1, in the renal tissue of various study groups. (frontiersin.org)
- In rat renal tissue, pioglitazone treatment decreased the serum levels of post-renal IRI creatinine and urea nitrogen, as well as necrosis. (frontiersin.org)
- After the end of reperfusion phase mice were sacrificed, blood samples were collected directly from the heart for determination of serum TNF-a, IL-6, urea and Creatinine. (biomedcentral.com)
- Serum creatinine may not become elevated before substantial kidney function has been lost due to renal reserve. (medscape.com)
- Serum creatinine measurement is not reliably indicative of underlying pathophysiology (ie, it does not allow differentiation of hemodynamically mediated changes in kidney function, such as prerenal azotemia from intrinsic renal failure or obstructive uropathy from structural kidney damage). (medscape.com)
- Serum and renal tissue samples were collected 24 hours after the last treatment and subjected to biochemical and histological examinations, respectively. (ac.ir)
- [ 1 ] At present, serum creatinine, which is used to measure the glomerular filtration rate (GFR), is the most commonly used marker of renal function. (medscape.com)
Ferroptosis5
- In the present study, the team was able to show that Liproxstatin-1 suppresses ferroptosis both in human kidney cells, Gpx4 deletion-induced acute renal failure and in a pre-clinical model of hepatic ischemia reperfusion injury. (sciencedaily.com)
- Recent studies have shown that ferroptosis is closely related to the pathophysiological processes of many diseases, such as tumors, nervous system diseases, ischemia-reperfusion injury, kidney injury, and blood diseases. (nature.com)
- Ferroptosis plays an important regulatory role in the occurrence and development of many diseases, such as tumors, neurological diseases, acute kidney injury, ischemia/reperfusion, etc. (nature.com)
- In 2012, Dixon 1 first proposed the concept of ferroptosis, an iron-dependent, non-apoptotic mode of cell death characterized by the accumulation of lipid reactive oxygen species (ROS). (nature.com)
- Liproxstatin-1 is a potent inhibitor of ferroptosis, a non-apoptotic form of cell death characterized by iron-dependent accumulation of lethal lipid reactive oxygen species (ROS). (abmole.com)
Cytokines1
- Moreover, upregulation in the expression of the inflammatory genes (TLR4, Myd88, and NLRP3), and overexpression of the pro-inflammatory cytokines (IL-1β), apoptotic (caspase-3) and pyroptotic (caspase-1) markers were observed in I/R-experienced animals. (bvsalud.org)
Ischemic reperfusion2
- The production of antioxidant enzymes that scavenge free radicals in ischemic tissue is then impaired, thereby exacerbating the damage caused by these free radicals in the post ischemic reperfusion tissue. (frontiersin.org)
- Testicular torsion is an urology emergency and which threats 1/4000 of male population younger than 25 years old with infertility as a sequela of germ cell ischemic/reperfusion (I/R) injury [ 1 ]. (biomedcentral.com)
Signaling pathway3
- In this study, we investigated the role of the NF-κB signaling pathway and inflammation in the amelioration of renal IRI using pioglitazone. (frontiersin.org)
- Thus, pioglitazone ameliorates renal IRI by inhibiting the NF-κB signaling pathway and inflammatory response in rats. (frontiersin.org)
- Therefore, in this study, we aimed to investigate the molecular mechanisms underlying pioglitazone hydrochloride-mediated amelioration of renal IRI with respect to the nuclear factor-kappaB (NF-κB) signaling pathway and inflammatory response using a rat model. (frontiersin.org)
Bilateral renal2
- Sprague-Dawley (SD) rats were subjected to bilateral renal artery clamping for 45 min followed by perfusion restoration for establishing a simulated renal IRI model. (frontiersin.org)
- A total of 24 Adult males of Swiss albino mice were randomized to four groups: I/R group (n = 6), mice underwent 30 minute bilateral renal ischemia and 48 hr reperfusion. (biomedcentral.com)
Evaluation of renal1
- The histopathological evaluation of renal tissue was also conducted. (frontiersin.org)
Tissues2
- The flow cytometry analysis demonstrated an elevated expression of innate and mature dendritic cells in I/R renal tissues. (bvsalud.org)
- However, reperfusion following a period of prolonged ischemia can often cause myocardial ischemia-reperfusion (I/R) injury, leading to damage of cardiac tissues ( 5 ). (spandidos-publications.com)
Rats were subjected1
- Adult rats were subjected to myocardial ischemia/reperfusion (I/R) injury with or without ischemic preconditioning (IPC), and the level of miR‑133b‑5p in myocardium was measured. (spandidos-publications.com)
Damage induced1
- The microvascular and parenchymal organ damage induced upon ischemia tissue reperfusion is mainly attributed to the reactive oxygen-free radicals, and it has been demonstrated in many organs. (frontiersin.org)
Mice4
- Sham group (n = 6), mice underwent same anesthetic and surgical procedures except for ischemia induction. (biomedcentral.com)
- The mice were treated with 45 min unilateral renal ischemia and reperfusion (I/R) to induce an acute renal injury. (oncotarget.com)
- One week later, the left renal artery was clamped for 30 min to cause ischemia-reperfusion injury, and mice were injected with iohexol using a retro-orbital injection method. (asn-online.org)
- I/R injury significantly impaired renal function in control mice as BUN and creatinine levels increased from 54.2? (liveconscience.com)
Cardiac4
- Studies of renal transplant recipients and patients undergoing cardiac surgery suggest that damage and inflammation caused by IRI and CPB is amplified by complement activation 4 . (nature.com)
- The term cardiorenal syndrome (CRS) is often used to describe this condition and represents an important model for exploring the pathophysiology of cardiac and renal dysfunction. (biomedcentral.com)
- The current definition has been expanded into five subtypes whose etymology reflects the primary and secondary pathology, the time frame, as well as cardiac and renal co-dysfunction secondary to systemic disease [ 1 ] (Table 1 ). (biomedcentral.com)
- Reperfusion after cardiac ischemia increases cell death and infarct size (IS), called myocardial ischemia/reperfusion (I/R) injury, which is the main cause of myocardial injury during the cardiac surgery particularly in coronary artery bypass graft surgery ( 1 , 2 ). (spandidos-publications.com)
Acute kidney5
- Renal ischemia-reperfusion injury (IRI) is a major cause of acute kidney injury (AKI). (mdpi.com)
- Renal ischemia-reperfusion injury (IRI) is considered as a major cause of acute kidney injury. (frontiersin.org)
- Acute kidney injury (AKI) is a common clinical syndrome characterized by rapid deterioration of renal function. (biomedcentral.com)
- In-hospital mortality, long-term mortality, acute kidney injury (AKI), and the requirement of continuous renal replacement therapy (CRRT) were assessed as endpoints. (biomedcentral.com)
- SUMMARY: One of the reasons for acute kidney damage is renal ischemia. (bvsalud.org)
Injury induced1
- Therefore, we were curious to know if Anwu can have a protective effect on remote organ injury after II/R. The purpose of the present study was to examine the effect of Anwu on the remote renal injury induced by II/R in rats and investigate its mechanism. (phcog.com)
Induce3
- Ischemia/ reperfusion was found to affect renal function and induce histopathological alterations. (bvsalud.org)
- Grupo I/R + diacereína, inyectado por vía intraperitoneal con 50 mg de diacereína/kg i.m. 30 min antes de la operación I/R. Se encontró que la isquemia/ reperfusión afecta la función renal e induce alteraciones histopatológicas. (bvsalud.org)
- Results CK2α manifestation is improved during renal I/R injury To induce I/R injury AKT inhibitor VIII manufacture the renal pedicles were bilaterally clamped for 25?min after which they were reperfused for various time periods (Fig. 1A). (liveconscience.com)
Post renal1
- It is considered as a major cause of acute renal failure and the main factor in early recovery of renal graft function and long-term survival post renal transplantation. (frontiersin.org)
Mitochondrial1
- Meanwhile, the enhanced inflammatory markers of TNF-α, IL-1β, IL-6, and IL-18 and apoptotic markers of Bax and caspase-3 were all significantly blunted by inhibiting mitochondrial complex-1. (oncotarget.com)
Inflammatory3
- Anwu has a protective effect against the II/R-induced remote renal injury in rats, which may be related to its regulation of antioxidant, anti-inflammatory, and antiapoptotic pathways. (phcog.com)
- This is followed by fragmentation into apoptotic bodies that are quickly removed by phagocytes, thereby preventing an inflammatory response. (wikipedia.org)
- Furthermore to its apoptotic inhibiting features a genuine amount of research have got suggested a pro-inflammatory function for CK2. (liveconscience.com)
20221
Tissue damage3
- Ischemia due to early hypoperfusion followed by ischemia reperfusion injury (IRI) results in renal tissue damage 4 . (nature.com)
- In addition to ischaemic tissue damage, seen for example in renal failure, stroke or cardiovascular diseases, the mechanism may also play a central role in neurodegenerative diseases and in comorbidities of diabetes mellitus. (sciencedaily.com)
- Restoration of blood supply, termed reperfusion, has been used to treat ischemic myocardium and prevent further tissue damage. (spandidos-publications.com)
Sham1
- After reperfusion or sham operation, blood and kidneys were collected from the rats for the detection of relative biochemical parameters. (phcog.com)
Caspase2
- The expression levels of the apoptotic protein, cleaved caspase-3, were raised, while the B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated x protein (Bax) ratio was reduced. (phcog.com)
- The present study was designed to investigate the role of the miR‑133b‑5p in HPC‑induced cardioprotection and the underlying mechanisms involving caspase‑8 and caspase‑3 apoptotic signaling. (spandidos-publications.com)
Cardiovascular1
- The trial had to exhibit at least one of the following results: major adverse cardiovascular events (MACE), cardiovascular death or hospitalization for heart failure, cardiovascular death, hospitalization for heart failure, renal or cardiovascular adverse events, or non-cardiovascular death. (bvsalud.org)
Hemodynamically1
- Hemodynamically mediated acute renal failure. (bmj.com)
Physiology1
- I discovered that a good place to begin in understanding kidney disease is a thorough review of basic renal physiology. (drprincetta.com)
19961
- 1996). Resistance to apoptotic cell death in a drug resistant T cell leukaemia cell line. (sciendo.com)
Hypoxia1
- In the present work, a hypoxia/reoxygenation (H/R) model in NRK-52E cells and ischemia-reperfusion model in rats were used. (mdpi.com)
Erythropoietin1
- Renal anemia is due to decreased erythropoietin levels. (drprincetta.com)
Protein1
- This protocol was revised from previously reported methods4 26 27 We 1st determined protein levels of CK2 in reperfused renal cells (Fig. 1B). (liveconscience.com)
Therapeutic approaches1
- Although pharmacological therapy, modern dialysis techniques, and intermittent or continuous renal replacement therapies exist, the therapeutic approaches for AKI remain very limited. (biomedcentral.com)
Cell5
- During a normal embryologic processes, or during cell injury (such as ischemia-reperfusion injury during heart attacks and strokes) or during developments and processes in cancer, an apoptotic cell undergoes structural changes including cell shrinkage, plasma membrane blebbing, nuclear condensation, and fragmentation of the DNA and nucleus. (wikipedia.org)
- Cell permeablity permits direct visualization of cytosolic apoptotic events. (immunochemistry.com)
- Apoptotic cell population does not diminish over time. (immunochemistry.com)
- Cell-based therapy with mesenchymal stem cells or renal progenitor cells (RPCs) resident in the kidney has appeared to be an effective strategy for the treatment of AKI. (biomedcentral.com)
- Cellular injury was evaluated by detecting cell viability, lactate dehydrogenase (LDH) activity and apoptotic rate. (spandidos-publications.com)
Transplantation2
- One of the primary causes of ARF is I/R which is a drop in blood flow leading to inadequate supply of oxygen and nutrients to renal tissue which can be caused by, amongst others, surgery, organ transplantation and shock [ 1 ]. (biomedcentral.com)
- kidney damage (AKI) following ischemia reperfusion (We/R) might negatively affect the results of kidney transplantation. (liveconscience.com)
Function2
- In our previous study, we found that Anwu can improve the intestinal function after ischemia/reperfusion. (phcog.com)
- Next, we examined the renal function-related markers and renal histology to assess the therapeutic effect of the injected cells. (biomedcentral.com)
Significantly1
- Renal indexes were not significantly different among all groups. (phcog.com)
Model1
- We then established the rat ischemia-reperfusion injury (IR) model and transplanted the iPSC-derived RPCs into the injured rats in combination with the hydrogel. (biomedcentral.com)
Assess1
- The objective of present study was to assess the effects of MK-886 and DITPA on renal I/R injury. (biomedcentral.com)