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  • pathways
  • CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. (cellsignal.com)
  • Because EGFR is at the origin of pathways governing diverse cell behavioral responses such as cell survival, proliferation, differentiation, and motility, ascertaining quantitative and dynamic features of the various regulatory pathways will be imperative for determining which features are related to particular responses. (mcponline.org)
  • Phosphorylation of AKT at both T308 and S473 fully activates AKT function through downstream signaling to mediate cell proliferation, survival, migration, angiogenesis and other pathways which are critical for tumor progression. (aacrjournals.org)
  • adenosine
  • Cystic fibrosis (CF) is a channelopathy caused by loss-of-function mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, which encodes a phosphorylation-activated and adenosine triphosphate (ATP)-gated chloride channel. (rupress.org)
  • interferon
  • Signal transducers and activators of transcription (STATs) and interferon regulatory factors (IRFs) share common target genes. (asm.org)
  • Many of the genes pertaining to this category contain a binding site for IFN regulatory factors (IRF), which is contained within the interferon-stimulated response element (ISRE) sequence ( 40 , 45 ). (asm.org)
  • degradation
  • Furthermore, the negative effect of cofilin on cellular F-actin contents was inhibited by co-expression of v-Src, whereas that of cofilin mutant Y68F (Y68 mutated to F) was not affected, suggesting that v-Src-mediated cofilin phosphorylation at Y68 is required for degradation of cofilin in vivo . (pubmedcentralcanada.ca)
  • In order to ascertain whether this N-terminal seryl residue is, indeed, the in vivo regulatory phosphorylation site, [ 32 P]phosphopeptides were isolated and purified from in vivo 32 P-labeled maize and sorghum leaf PEPC and subjected to automated Edman degradation analysis. (unl.edu)
  • This phosphorylation results in recognition of IκBα by a specific multicomponent ubiquitin ligase (β-TrCP-E3RS IκB -FWD1), leading to its polyubiquitination and eventual degradation by the 26S proteasome ( 15 , 52 ). (pubmedcentralcanada.ca)
  • transcription
  • The liberated NF-κB dimers translocate to the nucleus, where they are subjected to additional regulatory events that enhance their ability to activate transcription of genes that contain κB sites in their promoters/enhancers ( 12 ). (pubmedcentralcanada.ca)
  • results
  • The results show that purified light-form maize PEPC contains 14-fold more 32 P-radioactivity than the corresponding dark-form enzyme on an equal protein basis and, more notably, only a single N-terminal serine residue (Ser-15 in maize PEPC and its structural homolog, Ser-8, in the sorghum enzyme) was found to be 32 P-phosphorylated in the light or dark. (unl.edu)
  • contrast
  • A proposed phosphorylation site at codon 3291 affecting function was confirmed by substitution of an acidic residue (glutamate, BRCA2 Δex11/S3291E ) for the native serine, but in contrast to a prior report, phosphorylation was dispensable (alanine, BRCA2 Δex11/S3291A ) for BRCA2 -governed cellular phenotypes. (aacrjournals.org)
  • role
  • While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. (cellsignal.com)
  • Quantitative features, including intensity, timing, and duration of phosphorylation of particular residues, may play a role in determining cellular response, but experimental data required for analysis of these features have not previously been available. (mcponline.org)
  • actin
  • it was found that the assembly of myosin II filaments, but not actin microfilaments, depends on the myosin regulatory light chain (MLC-4) and essential light chain (MLC-5). (sdbonline.org)
  • Identification
  • The data set of quantitative temporal phosphorylation profiles was further characterized by self-organizing maps, which resulted in identification of several cohorts of tyrosine residues exhibiting self-similar temporal phosphorylation profiles, operationally defining dynamic modules in the EGFR signaling network consistent with particular cellular processes. (mcponline.org)
  • critical
  • However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). (cellsignal.com)
  • Mutagenesis of varicella-zoster virus glycoprotein I (gI) identifies a cysteine residue critical for gE/gI heterodimer formation, gI structure, and virulence in skin cells. (jove.com)
  • essential
  • Mutagenesis of varicella-zoster virus glycoprotein B: putative fusion loop residues are essential for viral replication, and the furin cleavage motif contributes to pathogenesis in skin tissue in vivo. (jove.com)