Regulatory elements. Medical search. Frequent questions

Through suppressive subtractive hybridization, we identified a new gene whose transcription is induced by sterol regulatory element-binding proteins (SREBPs). (elsevierpure.com)

Trans-acting factors interact with cis-regulatory elements to regulate gene expression. (wikipedia.org)
Trans- and cis-regulatory elements co-evolved rapidly in large-scale to maintain gene expression. (wikipedia.org)
TRE is more evolutionary constraint than cis-regulatory element, suggesting a hypothesis that TRE mutations are corrected by CRE mutations to maintain stability in gene expression. (wikipedia.org)
DNA binding trans-acting factors regulate gene expression by interfering with the gene itself or cis-acting elements of the gene, which lead to changes in transcription activities. (wikipedia.org)
Since many of these mutations affect elements that control the expression of key determinants of cell fate and phenotype, understanding how these mutations affect the networks that control gene expression opens the way for the development of new classes of drugs. (umassmed.edu)
Although the role of p53 as a strong trans-activator of gene expression is well known, the co-regulatory factors and local sequences acting at p53-bound CREs are comparatively understudied. (whiterose.ac.uk)
To elucidate the basis of the down-regulation in major histocompatibility complex (MHC) class I gene expression and to identify possible DNA-binding regulatory elements that have the potential to interact with class I MHC genes, we have studied the transcriptional regulation of class I HLA genes in human breast carcinoma cells. (nus.edu.sg)
Furthermore, the presence of such a regulatory element within the HLA class I gene whose activity can be modulated by hormones correlates well with our observation that the level of HLA class I gene expression can be down-regulated by hormones in human breast carcinoma cells. (nus.edu.sg)
Comparative analysis of these regulatory elements revealed the use of multiple TSSs, identical sequence motifs of promoter and Shine-Dalgarno sequence, reflecting conserved gene expression apparatuses between the two species. (ucsd.edu)
The readout of current genomic approaches such as DNase-seq, ATAC-seq or ChIP-seq for some histone modifications are widely used as surrogate markers for active regulatory elements, that control gene expression in a tissue-specific manner. (biologists.com)
Genomic cis regulatory elements support the gene transcriptional landscape which fine tune spatiotemporal gene expression via interaction with different transcription factors and co modulators during development. (fortuneonline.org)
These regulatory elements are poorly conserved, highly heterogenous with limited understanding of their role in gene expression. (fortuneonline.org)
CAMTAs participate in gene expression regulation by binding to the cis -elements in the promoter regions of numerous target genes. (frontiersin.org)
A cis regulatory element in the TAPNAC promoter directs tapetal gene expression. (tamu.edu)
In spite of the importance of this tissue in the quality and production of pollen grains, studies on promoter gene regulation of tapetal expressed genes are very few and there are no reports on specific cis regulatory sequences that control tapetal gene expression. (tamu.edu)
Xiong, W , Tapprich, WE & Stanley Cox, G 2002, ' Mechanism of gonadotropin gene expression: Identification of a novel negative regulatory element at the transcription start site of the glycoprotein hormone α-subunit gene ', Journal of Biological Chemistry , vol. 277, no. 43, pp. 40235-40246. (nebraska.edu)
Unoccupied thyroid hormone receptor binds to the response element within the regulatory region of target genes to __________ gene expression (i.e., transcription). (physiologyweb.com)
LCT gene expression is controlled by a DNA sequence called a regulatory element, which is located within a nearby gene called MCM6 . (medlineplus.gov)

Our data indicate that p53-bound CREs are both positively and negatively affected by alterations in local sequence context and changes to co-regulatory TF motifs. (whiterose.ac.uk)
662 conserved promoters having TSSs in both species resulted in the same number of comparable 5' UTR pairs, and that regulatory element was found to be the most variant region in sequence among promoter, 5' UTR, and ORF. (ucsd.edu)
Our data indicate that Drosophila UCEs are hubs for gene regulatory functions and suggest that UCE sequence invariance originates from their combinatorial roles in gene control. (figshare.com)
4462 functional sequence variations in regulatory DNA regions in training dataset F1 and 1116 functional SNVs in test datasets F2. (lu.se)

Dr. Lisa Dailey FIREWACH: A Functional approach for elucidating transcriptional regulatory elements within mammalian genomes. (cuny.edu)
FIREWACH: A Functional approach for elucidating transcriptional regulatory elements within mammalian genomes. (cuny.edu)

Additionally, each trans-regulatory element affects a large number of genes on both alleles, while cis-regulatory element is allele specific and only controls genes nearby. (wikipedia.org)
Exonic and promoter sequences of the genes are significantly more conserved than the genes in cis- and trans- regulatory elements. (wikipedia.org)
Trans-acting factors can be categorized by their interactions with the regulated genes, cis-acting elements of the genes, or the gene products. (wikipedia.org)
They are recruited to cis-regulatory Ets-IRF composite elements by PU.1 or Spi-B. How these IRFs target genes in most T cells is enigmatic given the absence of specific Ets partners. (nih.gov)
A 9 base pair (bp) negative cis-regulatory element (NRE) has been identified using band-shift assays employing DNA sequences derived from the 5'-flanking region of HLA class I genes. (nus.edu.sg)
However, expressed orthologous genes in E. coli and K. pneumoniae showed a strikingly different organization of upstream regulatory regions with only 20% identical promoters with TSSs in both species. (ucsd.edu)
Moreover, we find that the distal NDRs are enriched specifically for binding motifs of the pluripotency and germ cell master regulators such as NANOG, SOX17, AP2γ and OCT4 in human FGCs, indicating the existence of a delicate regulatory balance between pluripotency-related genes and germ cell-specific genes in human FGCs, and the functional significance of these genes for germ cell development in vivo . (nature.com)
We then surveyed the distribution of stress-related cis -regulatory elements in the −1.5 kb promoter regions of ZmCAMTA genes. (frontiersin.org)
Notably, a large number of stress-related elements present in the promoter regions of some ZmCAMTA genes, indicating a genetic basis of stress expression regulation of these genes. (frontiersin.org)
The role of de novo mutations in regulatory elements affecting genes associated with developmental disorders, or other genes , has been essentially unexplored. (bvsalud.org)
Some researchers have proposed that the vanH , vanA , and vanX genes of hospital enterococci may have been acquired en bloc from the actinomycetes ( 8 ). (cdc.gov)

Trans-regulatory elements (TRE) are DNA sequences encoding upstream regulators (ie. (wikipedia.org)
Chromatin immunoprecipitation sequencing in T helper 17 (T(H)17) cells reveals that IRF4 targets sequences enriched for activating protein 1 (AP-1)-IRF composite elements (AICEs) that are co-bound by BATF, an AP-1 factor required for T(H)17, B, and dendritic cell differentiation. (nih.gov)
Here, we develop ChromTransfer, a transfer learning method that uses a pre-trained, cell-type agnostic model of open chromatin regions as a basis for fine-tuning on regulatory sequences. (ku.dk)
The TAPNAC promoter was studied in detail to identify cis regulatory sequences that confer tapetal specific expression. (tamu.edu)
Transposable elements refer to DNA sequences capable of moving from one part of the genome to another. (lu.se)

Raquel Garza Gómez defends her Ph.D. thesis "Transposable elements in the healthy and diseased human brain ' on Friday, January 19th. (lu.se)
With a background in computational biology, Raquel's research focuses on studying the role of transposable elements in the human brain. (lu.se)
During my Ph.D. studies, my focus has been analyzing sequencing data in relation to transposable elements. (lu.se)
My research questions have explored different aspects of transposable elements, including their transcription and epigenetic regulation. (lu.se)
This is important for the understanding of human brain evolution as well as diseases with complex networks where transposable elements may play a regulatory role. (lu.se)
Thanks to technological advances in recent decades, more researchers like us have been able to dig deeper into the role of transposable elements in disease contexts and answer big questions about evolution and brain development. (lu.se)
During my Ph.D., my research has primarily focused on studying transposable elements in the human brain in various contexts such as development, healthy adulthood, and certain disease contexts. (lu.se)
My focus has been on the data analysis side of things, employing different computational methods to deal with mapping ambiguity and adapting new technologies such as single-cell RNA sequencing to better understand three families of transposable elements. (lu.se)
We hypothesize that a key event following loss of DNA methylation in glioma is the activation of transposable elements (TEs). (lu.se)

Understanding human genome implies discovering and characterizing all the functional elements encoded within. (umassmed.edu)
Genome wide association studies (GWAS) have identified mutations in non-coding regulatory regions as the most common features associated with human disease. (umassmed.edu)
For example, the algorithm REDUCE (Regulatory Element Detection Using Correlation with Expression) avoids subgroup selection in a natural way by genome-wide fitting of motif counts to expression data [ 15 ]. (biomedcentral.com)
Comparative Analysis of Regulatory Elements between Escherichia coli and Klebsiella pneumoniae by Genome-Wide Transcription Start Site Profiling. (ucsd.edu)
Here we use a well-known human tumor suppressor gene, Breast Cancer Type 1 ( BRCA1 ) and UCSC human genome browser database to report the in-silico putative cis regulatory enhancer element and its features. (fortuneonline.org)
We estimate that, genome -wide, 1-3% of patients without a diagnostic coding variant carry pathogenic de novo mutations in fetal brain -active regulatory elements and that only 0.15% of all possible mutations within highly conserved fetal brain -active elements cause neurodevelopmental disorders with a dominant mechanism. (bvsalud.org)
A Whole-Genome Analysis Framework for Effective Identification of Pathogenic Regulatory Variants in Mendelian Disease. (lu.se)

Ultraconserved elements (UCEs) are discrete genomic elements conserved across large evolutionary distances. (figshare.com)
We find 116 887 and 137 557 nucleosome-depleted regions (NDRs) in human and mouse FGCs, covering a large set of germline-specific and highly dynamic regulatory genomic elements, such as enhancers. (nature.com)

This 9-bp element, GTCATGGCG, located within exon 1 of the HLA class I gene, can potently inhibit the expression of a heterologous thymidine kinase (TK) gene promoter and the HLA enhancer element. (nus.edu.sg)

This accentuates the significance of genetic divergence within species due to cis- and trans-regulatory variants. (wikipedia.org)
abstract = 'Dysfunction of regulatory elements through genetic variants is a central mechanism in the pathogenesis of disease. (ku.dk)
Assessing the regulatory potential of human genetic variation: use of Massively Parallel Reporter Assays to identify >30,000 SNPs that modulate reporter expression. (biologists.com)
Our findings represent a robust estimate of the contribution of de novo mutations in regulatory elements to this genetically heterogeneous set of disorders, and emphasize the importance of combining functional and evolutionary evidence to identify regulatory causes of genetic disorders . (bvsalud.org)

Here we present GeneCodis 4, a new release that expands the functional analysis provided by the application, to accept regulatory elements, including lists of, transcription factors, CpG sites and miRNAs. (biorxiv.org)
225 Functional regulatory SNVs in monogenic and complex diseases and 241910 SNVs from dbSNP as a negative control dataset. (lu.se)
Prediction of functional regulatory SNPs in monogenic and complex disease. (lu.se)
This project is based on the hypothesis that LINE-1 elements constitute critical entities in glioma initiation and progression, by acting as a source of regulatory elements and coopted functional RNAs in tumor cells. (lu.se)

We identified de novo mutations in three classes of putative regulatory elements in almost 8,000 patients with developmental disorders. (bvsalud.org)

Given the complexity of human transcriptional regulation in vivo and the multitude of potential epigenetic mechanisms cooperating to regulate transcriptional activity, questions remain about the interplay of regulatory mechanisms in patients with CMML. (nature.com)
Interferon regulatory factor 4 (IRF4) and IRF8 regulate B, T, macrophage, and dendritic cell differentiation. (nih.gov)
IRPs regulate expression of ferritin and transferrin receptor at the mRNA level by interacting with a conserved RNA structure termed the iron-responsive element (IRE). (psu.edu)

These tracks show the known Drosophila melanogaster Reporter Constructs (RCs), Cis-Regulatory Modules (CRMs), and Transcription Factor Binding Sites (TFBSs) that have been annotated by version 9.5.1 of REDfly . (wustl.edu)
Rivera J, Keränen SVE, Gallo SM, Halfon MS. REDfly: the transcriptional regulatory element database for Drosophila . (wustl.edu)
Furthermore, we use a Drosophila cell culture system and transgenic Drosophila embryos to validate the notion of UCE combinatorial regulatory roles using an eUCE within the Hox gene Ultrabithorax and show that its protein-coding region also contains alternative splicing regulatory information. (figshare.com)

dblp: Identification of Conserved Regulatory Elements in Mammalian Promoter Regions: A Case Study Using the PCK1 Promoter. (dagstuhl.de)

Transcriptional regulation is modulated by a complex network of interactions between regulatory proteins and their binding targets on DNA. (biomedcentral.com)
Alterations in the interaction between iron regulatory proteins and their iron responsive element in normal and Alzheimer's diseased brains. (psu.edu)
Iron regulatory proteins (IRPs) are cytoplasmic mRNA binding proteins involved in intracellular regulation of iron homeostasis. (psu.edu)
Dive into the research topics of 'Alterations in the interaction between iron regulatory proteins and their iron responsive element in normal and Alzheimer's diseased brains. (psu.edu)

Our in-silico review on the features present within GH17J043079 element in BRCA 1 helps to postulate an intricate transcription regulation. (fortuneonline.org)
Such candidate based analysis of features within cis regulatory element on a gene can help elucidate intricate genomic architecture, gene regulation and its impact on complex disorders. (fortuneonline.org)

The ability to digest lactose into adulthood depends on which variants in the regulatory element within the MCM6 gene individuals have inherited from their parents. (medlineplus.gov)
27558 Mendelian disease regulatory variants from OMIM and ClinVar, 20963 complex disease regulatory variants from VarDi and NHGRI GWAS Catalog and 43364 recurrent cancer somatic variations. (lu.se)
Disease-associated variants in different categories of disease located in distinct regulatory elements. (lu.se)
Quantifying deleterious effects of regulatory variants. (lu.se)

The leucine operon leader is an RNA element that was found upstream of some leucine biosynthesis leuABCD operons in Escherichia coli and related bacteria and that is involved in the amino acid dependent attenuation of their transcription [ 6186194 ]. (lbl.gov)

Regulatory elements can include: CRMs and TF binding sites from REDfly and enhancers from FlyBase. (flymine.org)

This indicates that a majority of the regions under selection are non-coding regulatory elements or cis-regulatory elements. (umassmed.edu)

The participants represented 57 national medicines regulatory authorities, seven international organizations, and 12 international associations of patients, health professionals, pharmaceutical manufacturers and wholesalers. (who.int)
The World Health Organization (WHO) has carried out a survey2 on the situation of medicines regulatory authorities in the African Region. (who.int)

This genomic regulatory element and cognate factors appear to have evolved to integrate diverse immunomodulatory signals. (nih.gov)
To investigate the molecular basis for ectopic expression, it was critical to define cis-regulatory elements and their cognate trans-acting factors that modulate promoter activity in epithelial cell types that do not normally express GPH. (nebraska.edu)
consequently, this motif is referred to as the cap site diad element (CSDE) and the cognate factor as the cap site-binding protein (CSBP). (nebraska.edu)
These results indicate that a negative regulatory element centered at the GPHα gene cap site and its cognate DNA-binding protein make a significant contribution to the production of α-subunit in a variety of tumor tissues. (nebraska.edu)

Taken together our experiments indicate that UCEs emerge as a result of combinatorial gene regulatory roles and highlight common features in mammalian and insect UCEs implying that similar processes might underlie ultraconservation in diverse animal taxa. (figshare.com)

Other evolutionary models, such as the independent evolution of trans- or cis-regulatory elements, were deemed incompatible in regulatory systems. (wikipedia.org)
Co-evolution of the two regulatory elements was suggested to arise from the same lineage. (wikipedia.org)

To better understand disease etiology, there is consequently a need to understand how DNA encodes regulatory activity. (ku.dk)

Recent studies revealed pervasive binding of p53 to cis-regulatory elements (CREs), which are non-coding segments of DNA that spatially and temporally control transcription through the combinatorial binding of local transcription factors. (whiterose.ac.uk)
GH17J043079 showed histone activity in human embryonic stem cells, cancerous cells, housed transcription factors specific to liver cells and was enriched with Alu elements, indicative of ability for potential gene rearrangements. (fortuneonline.org)

Rapid kinetics of iron responsive element (IRE) RNA/iron regulatory pr" by Mateen A. Khan, Jia Ma et al. (cuny.edu)
We propose that elevated RNase activity is one mechanism by which the iron regulatory system becomes dysfunctional. (psu.edu)

The analysis of epigenetics patterns or regulatory elements have been an increasing focus of research in many contexts, such as development biology or characterization of molecular mechanisms in complex diseases ( Schübeler, 2015 ). (biorxiv.org)

In this work, we integrated somatic mutations, transcription, (hydroxy)methylation, histone modifications, and chromatin accessibility to reveal the complexity of the epigenetic landscape, the simultaneous presence of multiple regulatory mechanisms affecting drivers of leukemogenesis, and remodeling of the enhancer landscape as an important driver of intratumoral heterogeneity. (nature.com)
De novo mutations in regulatory elements in neurodevelopmental disorders. (bvsalud.org)
Here we show that de novo mutations in highly evolutionarily conserved fetal brain -active elements are significantly and specifically enriched in neurodevelopmental disorders . (bvsalud.org)

The cyclic adenosine monophosphate (cAMP) receptor protein CRP, which is the global regulator of CCR, binds to regulatory DNA elements called CRP sites when in complex with cAMP. (biomedcentral.com)

The systematic examination of evidence generated and procedures undertaken by the manufacturer, under requirements established by the Regulatory Authority, to determine that a medical device is safe and performs as intended by the manufacturer and, therefore, conforms to the Essential Principles of Safety and Performance of Medical Devices. (who.int)

The underlying mechanisms are discussed with respect to carbohydrate availability, trade-offs to competing demands as well as to regulatory elements. (mdpi.com)

In the study of genomic cis -regulatory elements, generation of mice featuring transgenic reporters or cis -regulatory element knockout remains the standard approach. (mdpi.com)

DNA-mediated transient expression of promoter-reporter constructs was used to identify a novel negative regulatory element located at the GPHα gene transcription start site. (nebraska.edu)

A medicines regulatory authority should have a national medicines policy and related legislation as well as norms, guidelines, procedures and adequate financial and human resources. (who.int)

Such interactions between negative regulatory elements and specific hormone trans-activators are novel and suggest a versatile form of transcriptional control. (nus.edu.sg)

Furthermore, it assesses compliance practices promoted by OEFA and provides recommendations to improve regulatory enforcement. (oecd-ilibrary.org)
Regulatory and enforcement elements for the national T21 law are unfolding and may have implications for the guidance provided in this document. (cdc.gov)

We also note that the gene regulatory roles of intronic and intergenic UCEs (iUCEs) are distinct from those found in exonic UCEs (eUCEs). (figshare.com)
Together, these findings demonstrate regulatory roles of ASHCEs in the creation of avian-specific traits, and further highlight the importance of cis-regulatory rewiring during macroevolutionary changes. (elsevierpure.com)

In recent years, Massively Parallel Reporter Assays (MPRAs) have allowed to assess promoter or enhancer activity in thousands of DNA fragments en masse moving the genomics field forward ( 2 ). (biologists.com)

By utilizing different sets of co-factors across CREs, we hypothesize that global p53 activity is guarded against loss of any one regulatory partner, allowing for dynamic and redundant control of p53-mediated transcription. (whiterose.ac.uk)
Truncation or site-directed mutagenesis of this element produced up to a 10-fold increase in promoter activity. (nebraska.edu)
Whereas the Healthy People 2010 definition is more comprehensive than merely considering any single health promotion activity, many identified best practice program-design elements are not included. (cdc.gov)

More interestingly, NRE can suppress dexamethasone-mediated gene activation in the context of the reported glucocorticoid responsive element (GRE) in MCF-7 cells but has no influence on the estrogen-mediated transcriptional activation of MCF-7 cells in the context of the reported estrogen-responsive element (ERE). (nus.edu.sg)

On the other hand, the major families of repetitive elements such as long-interspersed nuclear elements (LINEs), short-interspersed nuclear elements (SINEs) and α satellites still retain abundant residual DNA methylation ( ∼ 12%-37%), which may provide a basis for potential transgenerational epigenetic inheritance. (nature.com)

1. The Medicines Regulatory Authority (MRA) is the body in charge of coordinating and overseeing the pharmaceutical sector for the purpose of protecting public health. (who.int)
The regulatory system is managed by a Medicines Regulatory Authority (MRA) whose mission is to coordinate and oversee the medicines sector in order to protect public health. (who.int)

to identify enhancer elements (Image taken from figure 1a). (biologists.com)

This research describes a comprehensive examination of the cognitive, motivational, and emotional processes underlying active learning approaches, their effects on learning and transfer, and the core training design elements (exploration, training frame, emotion-control) and individual differences (cognitive ability, trait goal orientation, trait anxiety) that shape these processes. (cornell.edu)
We will then use CRISPR approaches to silence/activateLINE-1 elements in glioma cell lines and interrogate the consequences of LINE-1 modulation on tumor physiology. (lu.se)

Anatomy15

Organisms21

Diseases1

Chemicals and Drugs85

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes115

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science7

Sterol Regulato1

Gene expression18

Sequence4

Transcriptional regulatory elements2

Genes11

Sequences5

Transposable elements9

Genome7

Genomic elements2

Enhancer element1

Genetic4

Functional4

Putative1

Regulate3

Drosophila3

Identification1

Proteins4

Regulation2

Variants4

Upstream1

CRMs1

Regions1

Medicines regulatory authorities2

Cognate4

Mammalian1

Evolution2

Encodes1

Transcription factors2

Iron regulatory2

Epigenetics1

Mutations3

Binds1

Systematic1

Mechanisms1

Mice1

Constructs1

National medicines1

Interactions1

Enforcement2

Roles2

Assays1

Activity3

Cells1

Methylation1

Authority2

Identify1

Approaches2