Regulatory adenovirus e1a proteins. Medical search. Frequent questions

This region of the viral genome encodes a series of related proteins with multifunctional capabilities. (bdbiosciences.com)
The adenovirus type 5 (HAdV-C5) E1 transcription unit encodes regulatory proteins that are essential for viral replication and transformation. (nih.gov)
This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. (nih.gov)
key target for these transcription factors is cdc18 gene, which encodes protein required for initiation of DNA replication (see text). (comprehensivephysiology.com)
Human cytomegalovirus (HCMV) is a member of the betaherpesvirus subfamily and encodes more than 200 viral proteins (Chee et al. (springer.com)
The rep gene, through the use of two promoters and alternative splicing, encodes four regulatory proteins that are dubbed Rep78, Rep68, Rep52 and Rep40. (vectorbiolabs.com)
The first gene encodes four proteins necessary for genome replication (Rep78, Rep68, Rep52, and Rep40), and the second expresses three structural proteins (VP1-3) that assemble to form the viral capsid. (justia.com)

Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA. (nih.gov)
In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. (nih.gov)
The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. (nih.gov)
Antisense molecules are a small, complementary, single-stranded type of DNA that binds targeted messenger RNA (mRNA) within the cell and prevents specific protein translation. (medscape.com)
This segment contains transcription ter- mination and polyadenylation sites for late transcripts as well as a negative regulatory element acting at the level of late mRNA stability [36,48]. (nih.gov)
Nonsense mutations or deletions causing frame shifts in the dystrophin mRNA, both of which create premature termination codons (PTCs), usually result in a severe DMD phenotype, because of a lack of the dystrophin protein. (nature.com)

The E1A and E1B regions together comprise the transforming region of adenovirus. (bdbiosciences.com)

Spry proteins are central and complex regulators of the receptor tyrosine kinase (RTK) signalling pathway. (biomedcentral.com)
Association of Human Cyclin E with a Periodic G.sub.1 -S Phase Protein Kinase", Science 257:1958-1961 (1992). (everypatent.com)
therapeutic_agents C1909 therapeutic_agents C C177537 GDC Value Terminology C128288 Luvixasertib An orally bioavailable, selective inhibitor of the dual specificity protein kinase TTK (monopolar spindle 1 kinase, Mps1), with potential antineoplastic activity. (nih.gov)
We suggest this negative relationship is mediated through elevated diacylglycerol production and chronic down-modulation of protein kinase C. Protein kinase C is known to be essential for expression of differentiation-related genes in keratinocytes. (nih.gov)
The central serine/threonine-kinase P-TEFb (CDK9-cyclin T), an AFF scaffold protein, and ENL or AF9 are consistent components of SEC complexes. (elifesciences.org)
Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. (lookformedical.com)
for example, protein kinase activation is part of the platelet activation signal pathway. (lookformedical.com)
It is known that the human Ras GTPase activating protein (GAP) p120-GAP can be phosphorylated by different members of the Src kinase family and recently phosphorylation of the GDP/GTP exchange factor (GEF) CDC25Mm/GRF1 by proteins of the Src kinase family has been revealed in vivo [Kiyono, M., Kaziro, Y. & Satoh, T. (2000) J. Biol. (embl.de)
In addition to its association with Ras, GAP has been shown to bind to several tyrosine-phosphorylated proteins in cells stimulated by growth factors or expressing transforming tyrosine kinase variants. (embl.de)

Esta proteína interacciona con proteínas reguladoras clave tales como la PROTEÍNA DE UNIÓN A TATA, la TFIIB y las PROTEÍNAS E1A DEL ADENOVIRUS. (bvsalud.org)
Structures of cyclins, Rb and TFIIB reveal that a common motif occurs in proteins regulating three consecutive events of cell-cycle control. (embl-heidelberg.de)
Recent results on the structures of two cyclins, cyclin A and cyclin H, and two transcription factor mediator proteins, TFIIB and the A pocket region of the retinoblastoma tumour suppressor protein (Rb), show that they share domains with a strikingly similar alpha-helical topology, despite remote sequence identity. (embl-heidelberg.de)
Solution structure of the C-terminal core domain of human TFIIB: similarity to cyclin A and interaction with TATA-binding protein. (embl-heidelberg.de)

CtBP also binds the Kruppel-like factors family of zinc finger proteins KLF3, KLF8 and KLF12. (wikipedia.org)
The fiber protein from most adenoviruses, except species B, binds to the cellular receptor CAR (coxsackie-adenovirus receptor), which also binds coxsackie B virus [ 2 ]. (medilib.ir)
Arp2-3 complex binds WASP PROTEIN and existing ACTIN FILAMENTS, and it nucleates the formation of new branch point filaments. (nih.gov)
HN - 2006 BX - Arp2-3 Complex MH - Actin-Related Protein 3 UI - D051378 MN - D5.750.78.730.246.750 MN - D12.776.220.525.246.750 MS - A component of the Arp2-3 complex that is related in sequence and structure to ACTIN and that binds ATP. (nih.gov)
The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. (nih.gov)

13. Epstein-Barr virus nuclear protein 2 interacts with p300, CBP, and PCAF histone acetyltransferases in activation of the LMP1 promoter. (nih.gov)
Retinoblastoma protein (Rb) interacts with cyclin-dependent kinases and regulates the transcription of genes necessary for progression through the S phase of the cell cycle. (embl-heidelberg.de)
The carboxyl terminus of the COUP-TF proteins interacts with multiple FOG2 zinc finger and FOG-2 serves as a co-repressor for COUP-TFII. (guidetopharmacology.org)

Using deletion mutants we identified the N-terminus and the CR conserved region (CR) 3 of E1A- and the C-terminal half of Spry1, which contains the highly conserved Spry domain, as the essential sites for direct interaction between Spry and E1A. (biomedcentral.com)
CR3 is one of four E1A regions (CR1, CR2, CR3 and CR4) that are highly conserved among all Adenovirus serotypes [ 2 ]. (biomedcentral.com)
Also highly conserved is the extensive A-B interface, suggesting that it may be an additional protein-binding site. (embl-heidelberg.de)

In a yeast two-hybrid screen we have identified Sprouty1 (Spry1) as a target of adenoviral E1A Oncoproteins. (biomedcentral.com)
The team focused on an adenoviral oncogene called E1A, and a protein that it codes for with the same name. (vetscite.org)

The fusion protein contained the C-terminal 266 amino acids of the 13S E1A protein. (bdbiosciences.com)

Among these, E1A and E1B-55K act as key multifunctional HAdV-C5 proteins involved in various steps of the viral replication cycle and in virus-induced cell transformation. (nih.gov)
We discuss in this review re-cent findings suggesting that B-Myb is a multifunctional protein that has, in addition to its transcriptional proper-ties, the ability to interact directly with other regulators of the cell cycle. (123dok.net)

Several other oncogenes, including the activated H-ras oncogene and polyoma middle T antigen, can complement E1A by substituting for E1B in transformation assays. (bdbiosciences.com)
The commercial assays for adenovirus antibodies, such as complement fixation and enzyme immunoassay, detect other adenovirus-specific antibodies that cross-react with all serotypes. (medilib.ir)
Here, we confirm our yeast two-hybrid data, showing the interaction between Spry1 and E1A in GST pull-down and immunoprecipitation assays. (biomedcentral.com)
SRE and TRE reporter assays demonstrated that co-expression of Spry1 with E1A 13S abolishes the inhibitory function of Spry1 in RTK signalling, which is consequently accompanied with a decrease of E1A 13S -induced gene expression. (biomedcentral.com)

Several conserved regions of E1A are strikingly similar to portions of other viral oncoproteins, e.g. (bdbiosciences.com)
Oncoproteins encoded by the early region of adenoviruses have been shown to be powerful tools to study gene regulatory mechanisms, which affect major cellular events such as proliferation, differentiation, apoptosis and oncogenic transformation. (biomedcentral.com)
These results establish Spry1 as a cytoplasmic localized cellular target for E1A oncoproteins to regulate the RTK signalling pathway, and consequently cellular events downstream of RTK that are essential for viral replication and transformation. (biomedcentral.com)
E1A oncoproteins cooperate with Ad early region 1B (E1B) oncogene products to transform rodent cells in culture and, depending on the serotype, to induce tumors in immunocompetent animals (e.g. (biomedcentral.com)

3. The histone acetyltransferase domains of CREB-binding protein (CBP) and p300/CBP-associated factor are not necessary for cooperativity with the class II transactivator. (nih.gov)
5. Acetylation of interferon regulatory factor-7 by p300/CREB-binding protein (CBP)-associated factor (PCAF) impairs its DNA binding. (nih.gov)
10. CREB-binding protein/p300 activates MyoD by acetylation. (nih.gov)
12. Histone acetyltransferase activity of p300 is required for transcriptional repression by the promyelocytic leukemia zinc finger protein. (nih.gov)
14. Acetylation by histone acetyltransferase CREB-binding protein/p300 of STAT6 is required for transcriptional activation of the 15-lipoxygenase-1 gene. (nih.gov)
16. The E1A proteins of all six human adenovirus subgroups target the p300/CBP acetyltransferases and the SAGA transcriptional regulatory complex. (nih.gov)
So konnte mit dem POD-assoziierten Transkriptionskoaktivator p300 erstmals ein zellulärer Interaktionspartner von E4orf3 identifiziert werden, der auch ein Zielprotein der E1A-Onkoproteine darstellt. (uni-regensburg.de)
Immunfluoreszenz- und funktionelle Promotor/Reporter-Analysen zeigen, dass das Ad5-Protein p300 in reorganisierte POD-Strukturen sequestriert und die transkriptionsaktiven Eigenschaften des Tumorsuppressorproteins inhibiert. (uni-regensburg.de)
Da auch die E1A-Proteine die p300-vermittelte Transkriptionsaktivierung blockieren, muss man annehmen, dass E4orf3 mit E1A redundante Aktivitäten aufweist. (uni-regensburg.de)
Dafür spricht auch die Beobachtung, dass die Binderegionen von E1A und E4orf3 im p300-Protein teilweise überlappen und die Tatsache, dass das Ad5 E4orf3-Protein eine p300-bindungsdefiziente, transformationsdefekte E1A-Mutante (E1delN) in BRK-Transformationsexperimenten komplementiert. (uni-regensburg.de)
Insgesamt zeigen diese Ergebnisse, dass die E4orf3-Proteine humaner Adenoviren eine zu E1A redundante Funktion besitzen und lassen vermuten, dass die onkogenen und mutagenen Eigenschaften des Ad5-Proteins über die Modulation der subnukleären Lokalisation und Funktion von p300 vermittelt werden. (uni-regensburg.de)
We report that transcription of the adenovirus 5 E4 region is regulated during the release of paused Pol2 into productive elongation by recruitment of the super-elongation complex, dependent on promoter H3K18/27 acetylation by CBP/p300. (elifesciences.org)

Because a co-infecting helper virus (such as adenovirus or herpes simplex virus) or induction of cellular stress is usually required for a productive infection to occur, AAV serotypes are ascribed to a separate genus in the Parvoviridae family designated Dependovirus. (vectorbiolabs.com)
The former develops in cells infected with a helper virus such as adenovirus or herpes simplex virus (HSV), whereas the latter is established in host cells in the absence of a helper virus. (vectorbiolabs.com)
When a latently infected cell is super-infected with a helper virus, such adenovirus or herpes simplex virus, the AAV gene expression program is activated leading to the AAV Rep-mediated rescue (i.e., excision) of the provirus DNA from the host cell chromosome, followed by replication and packaging of the viral genome. (vectorbiolabs.com)

[ 69 ] So far, nine CDKs and 16 cyclins have been identified and a specific combination of a particular CDK with its regulatory cyclin molecule forming an active complex is required for cell cycle progression at each stage. (medscape.com)
In addition to CDKIs, pRb and the related 'pocket' proteins p107 and p130 are some of the major negative regulators in controlling mammalian cell cycle progression. (medscape.com)
[ 75 ] These pocket proteins, in their active hypophosphorylated state as found in quiescent cells (G0 phase), block cell cycle progression through interactions with the E2F family of transcription factors. (medscape.com)

The team's findings are the latest in a long and distinguished line of research at CSHL involving adenovirus, a type of virus that causes the common cold in people, but whose genome contains known oncogenes - genes whose expression can promote cancer under certain conditions. (vetscite.org)
GTP-binding proteins as oncogenes in human tumors. (nih.gov)
Although protein-relevant therapeutics such as antibodies against Programmed Cell Death 1 (PD1), programmed death-ligand 1 (PDL1), and cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) have driven a revolutionary trend in pharmacotherapy and drug development, some protein targets encoded by oncogenes are undruggable or inadequate for achieving remission, and cancer cells can acquire drug resistance [ 1 ]. (biomedcentral.com)

PSMC1 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides. (nih.gov)
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. (lookformedical.com)

That is, regulatory proteins that bind to sequence-specific DNA-binding proteins and help turn genes off. (wikipedia.org)
Group B adenoviruses have been shown to bind to CD46, a complement-related protein [ 3,4 ]. (medilib.ir)
Coupled to the outside are "protein arms" that bind short RNA helices and embed them. (vetscite.org)
AN - coordinate IM with ADENOMA (IM) HN - 2006 BX - Corticotroph Adenoma BX - Pituitary Adenoma, ACTH-Secreting BX - Pituitary Corticotropin-Secreting Adenoma MH - Actin Capping Proteins UI - D051344 MN - D5.750.78.730.32 MN - D12.776.220.525.32 MS - Actin capping proteins are cytoskeletal proteins that bind to the ends of ACTIN FILAMENTS to regulate actin polymerization. (nih.gov)

Two species resulting from the 12S and 13S E1A mRNAs have reduced molecular weights of 26 kDa and 32 kDa, respectively. (bdbiosciences.com)
These are (i) a polyadenylation site for late mRNAs, which is fairly close to the 5 end of the LCR, (ii) E2 protein binding sites, which vary in relative position and in number from 4 in genital HPVs to 10 in BPV-1, (iii) one E1 protein binding site, and (iv) the TATA box of the E6 gene promoter. (nih.gov)
Thus, internally deleted but partially functional dystrophin proteins can be produced from the exon-skipped mRNAs. (nature.com)

progres-sion through the cell cycle is highly modulated through a regulatory axis containing the E2F transcription factor and retinoblastoma tumour suppressor protein families. (123dok.net)

Emerging evidence demonstrates that some metabolic enzymes that phosphorylate soluble metabolites can also phosphorylate a variety of protein substrates as protein kinases to regulate cell cycle, apoptosis and many other fundamental cellular processes. (bvsalud.org)
In transformed cells, induction of apoptosis by adenovirus type 2 (Ad2) early region 4 ORF 4 (E4orf4) correlates with accumulation of E4orf4 in the cell membrane-cytoskeleton fraction. (silverchair.com)

The N terminus and the CRs of E1A mediate most of the gene regulatory functions necessary for viral reproduction and transformation [ 8 ]. (biomedcentral.com)
SH3 domains mediate many key regulatory processes in normal cells and are involved the pathomechanisms of many important diseases. (helsinki.fi)
It appears that many other viral proteins also share the capacity of Nef to utilize binding to host cell SH3 proteins in order to mediate their functions. (helsinki.fi)

When bound, it undergoes a conformational change that allows it to dimerize and associate with its partner proteins and silence specific genes. (wikipedia.org)
Proteins encoded by the early transcription unit 1A (E1A) of Adenovirus (Ad) are essential for the viral life cycle because of their necessity in regulating the expression of all other viral genes [ 1 ]. (biomedcentral.com)
Adenovirus carries a number of cooperating genes that modulate cell growth in ways we're interested in," said William Tansey, Ph.D., who, along with CSHL professors Scott Lowe, Ph.D., and Gregory Hannon, Ph.D., is one of the team's co-leaders and corresponding author of a paper in Proceedings of the National Academy of Sciences. (vetscite.org)
One of the genes regulated through this mechanism en-codes the B-Myb transcription factor, which has been shown to be critically required for early embryonal de-velopment in the mouse. (123dok.net)
We also establish that this is a general transcriptional regulatory mechanism that applies to ~7% of expressed protein-coding genes in primary human airway epithelial cells. (elifesciences.org)
The ITRs flank the two viral genes - rep (replication) an"yd cap (capsid), encoding non-structural and structural proteins, respectively. (vectorbiolabs.com)

They are possesing a key role to favor viral replication via their interaction with multiple cellular proteins. (biomedcentral.com)
We also demonstrated the interaction of E1A with two further Spry isoforms. (biomedcentral.com)
Identification of normal SH3-mediated cellular protein interaction networks as well as SH3-interactions that take place between cellular and pathogenic viral proteins, and elucidation of the molecular basis of binding affinity and specificity involved in these interactions are the main topics goals of our research. (helsinki.fi)
In addition, COUP-TF activates transcription through protein-protein interaction with DNA-bound factor, such as with HNF-4 in the HNF-1a gene promoter. (guidetopharmacology.org)

also known as ASH1), neurogenic differentiation factor 1 (NeuroD1), yes-associated protein 1 (YAP1) and POU class 2 homeobox 3 (POU2F3). (cancerindex.org)
These proteins play important roles regulating proliferation during normal development and differentiation and after genotoxic stress. (aacrjournals.org)

tumor_purity tumor purity C C177536 GDC Property Terminology C13202 Chromosome A structure found in cells that is comprised of a strand of linearized double-stranded DNA plus proteins that package the DNA in a condensed coil form and regulate chromosomal function. (nih.gov)
The accessory GTPase-activating proteins (GAPs) negatively regulate the cell signaling by increasing the slow intrinsic GTP to GDP hydrolysis rate of Ras. (embl.de)

Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. (wikipedia.org)
Human adenoviruses are widely used as models for studying cellular transformation and regulation of gene expression. (bdbiosciences.com)
Active cyclin/Cdk complexes phosphorylate and inactivate members of the retinoblastoma protein (Rb) family that are negative regulators of G 1 and S-phase progression, leading to induction of E2F-regulated gene expression and cell proliferation. (aacrjournals.org)
Proteins that enhance gene expression when associated with ligand bound activated NUCLEAR RECEPTORS . (nih.gov)

A protein which is a subunit of RNA polymerase. (nih.gov)

The vertebrate Myb transcription factors comprise a small family of important regulatory proteins. (123dok.net)
The present invention further provides recombinant AAV (rAAV) virions that comprise a mutant capsid protein. (justia.com)

Serotype differences - Over 60 human adenovirus serotypes have been described, based upon antigenic determinants detected by viral neutralization assay (51 serotypes) and other new "types" identified by molecular analyses [ 6 ]. (medilib.ir)

As its name implies, AAV is dependent upon the presence of a helper virus, such as an adenovirus or herpes virus, for active replication. (justia.com)

Recovery from adenovirus infection is associated with the development of serotype-specific neutralizing antibodies directed mainly at the major capsid protein hexon. (medilib.ir)
The cap gene, through alternative splicing and initiation of translation, gives rise to three capsid proteins, VP1 (virion protein 1), VP2 and VP3, with molecular weight of 87, 72 and 62 kDa, respectively. (vectorbiolabs.com)
These capsid proteins assemble into a near-spherical protein shell of 60 subunits. (vectorbiolabs.com)
The present invention further provides nucleic acids comprising nucleotide sequences that encode mutant capsid proteins, and host cells comprising the nucleic acids. (justia.com)

HN - 2006(1981) BX - Cofilins MH - Actin-Related Protein 2 UI - D051377 MN - D5.750.78.730.246.500 MN - D12.776.220.525.246.500 MS - A PROFILIN binding domain protein that is part of the Arp2-3 complex. (nih.gov)
HN - 2006(1998) MH - Actin-Related Protein 2-3 Complex UI - D051376 MN - D5.750.78.730.246 MN - D12.776.220.525.246 MS - A complex of seven proteins including ARP2 PROTEIN and ARP3 PROTEIN that plays an essential role in maintenance and assembly of the CYTOSKELETON. (nih.gov)

The idea that E1A is also capable of exerting its regulatory functions by directly affecting cytoplasmic processes was supported by the discovery that a certain amount of E1A proteins is acetylated at Lys 239 , which determines the cytoplasmic localization of E1A proteins by interfering with the nuclear transport [ 13 ]. (biomedcentral.com)
Cyclin: A Nuclear Protein Whose Level Correlates Directly with the Proliferative State of Normal As Well as Transformed Cells", Leukemia Res. (everypatent.com)
To determine the relative contribution of nuclear E4orf4 versus membrane-associated E4orf4 to cell death signaling, we engineered green fluorescent fusion proteins to target E4orf4 to specific cell compartments. (silverchair.com)
This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. (nih.gov)
Alternatively nuclear receptor coactivators can function as adaptor proteins that bring nuclear receptors into close proximity with transcriptional complexes. (nih.gov)

at least 60 E1A polypeptide species have been identified, ranging between 30 and 46 kDa. (bdbiosciences.com)
The fiber protein is attached noncovalently to the icosahedron by a pentameric polypeptide named penton base. (medilib.ir)
The major surface protein of the virion is the trimeric polypeptide hexon. (medilib.ir)

E1A regulates the transcriptional regulation of a wide variety of viral and cellular promoters. (bdbiosciences.com)
Regulates E3 ubiquitin-protein ligase activity of RNF19A (By similarity). (nih.gov)
p120 GTPase-activating protein (GAP) down-regulates Ras by stimulating GTP hydrolysis of active Ras. (embl.de)

In addition, CtBP complexes with CtIP, a 125 kDa protein that recognizes distinctly different protein motifs from CtBP. (wikipedia.org)
M58 recognizes Adenovirus Type 5 E1A proteins.4 A trpE-E1A fusion protein was used as immunogen. (bdbiosciences.com)

By extensive screening of 100,000 chemical compounds in a chemical library using in vitro phosphorylation assay, we identified several synthetic chemical compounds that inhibit SR protein kinases specifically. (nature.com)
We first identified a synthetic compound as a specific inhibitor of SR protein kinases and named it as SRPIN340 (ref. 21 ). (nature.com)

Selective AAV DNA encapsidation is presumably directed by protein-protein interactions between two complexes: (1) the pre-formed empty capsids and (2), complex of Rep78 or Rep68 with the virus genome. (vectorbiolabs.com)
This site-specific integration involves the AAV ITRs and Rep proteins (Rep78, Rep68). (vectorbiolabs.com)

The cell cycle is delicately controlled by complex mechanisms integrating many proteins to ensure correct cell division with duplication of the cellular genome into daughter cells. (medscape.com)
These proteins are involved in AAV genome replication. (vectorbiolabs.com)
Next, the Rep52 and Rep40 proteins are responsible for transferring the AAV genome DNA into empty particles through the pores. (vectorbiolabs.com)

yet, standard laboratory mice do not support infection with SARS-CoV-2 because the virus S protein is incompatible with the mouse-human receptor homolog (mACE2) [ 11 ]. (biomedcentral.com)

The HIV Nef protein has been a key subject of our research, Nef modifies host cell physiology to better support viral replication by binding to proteins involved in cellular signal structures via their SH3 domains. (helsinki.fi)

The CtBP1 protein was originally identified as a human protein that bound a PLDLS motif in the C-terminus of adenovirus E1A proteins. (wikipedia.org)
The 4X IRF3 construct contains four copies of the IRF3-binding positive regulatory domain (PRD)I/III motif of the IFNb promoter in front of a luciferase reporter gene. (sri.com)
The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. (cancerindex.org)
Cas9 protein -uses guide RNA (gRNA) to direct site-specific, double-strand DNA cleavage adjacent to a protospacer adapter motif (PAM) in the target DNA. (reportergene.com)

Our results suggest that phosphorylation by p60c-Src and Lck is a selective process that can modulate the activity of p120-GAP and CDC25Mm towards Ras proteins. (embl.de)

The C terminus comprises an autoregulatory domain (negative regulatory domain: NRD) that suppresses transactivation function [6, 8, 10 - 14]. (123dok.net)

Lysates from 293 human embryonic kidney cells were probed with anti-adenovirus Type 5 E1A (clone M58, Cat. (bdbiosciences.com)
293 human embryonic kidney cells or E1A/Adenovirus Type 5 infected HeLa cells are suggested as positive controls. (bdbiosciences.com)
The transforming potential of human adenoviruses has been traditionally ascribed to the E1A and E1B transcription units encoded within early region 1 (E1). (uni-regensburg.de)
Group-reactive antigenic determinants are present on the hexon proteins from all human adenoviruses. (medilib.ir)
Most adenoviruses readily infect human epithelial cell lines, exhibiting a characteristic cytopathic effect. (medilib.ir)
Prions, rogue proteins that cause incurable brain infections such as Mad Cow disease and its human equivalent, variant Creutzfeldt-Jakob Disease, are difficult to inactivate, resisting extreme heat, chemical disinfectants, and irradiation. (vetscite.org)
The first human adeno-associated virus (AAV) was discovered in 1965, as a contaminant of adenovirus (Ad) preparations, hence the name. (vectorbiolabs.com)
Transfection conditions were determined using enhanced green fluorescent protein as a reporter expressed under the transcriptional control of the human cytomegalovirus immediate early gene 1 promoter/enhancer. (biomedcentral.com)

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. (nih.gov)
Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (By similarity). (nih.gov)

Some of the new approaches depend on tumor biology and aim specifically to inhibit tumor growth and metastasis by targeting the tumor microenvironment or vasculature (leaving normal cells unaffected) or focusing on specific protein or signal transduction pathways. (medscape.com)

During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of δEF1, a cellular zinc finger-homeodomain protein, and thereby enhances δEF1-induced transcriptional silencing. (wikipedia.org)
A ubiquitously expressed zinc finger-containing protein that acts both as a repressor and activator of transcription. (nih.gov)

Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. (nih.gov)
Changes in the activity of DNA repair proteins during viral infection can cause disturbances in the DNA repair system and change its mechanisms. (springer.com)

1983) DNA Polymerase-a Cofactors C1C2 Function as Primer Recognition Proteins,J. Biol. (nih.gov)

While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. (bdbiosciences.com)
Die in der vorliegenden Arbeit dargestellten Untersuchungen zum transformierenden Potenzial humaner Adenoviren zeigen erstmals, dass zwei virale Genprodukte der frühen Region 4 - E4orf3 und E4orf6 - von Adenovirus Serotyp 5 transformierende Eigenschaften aufweisen, die zur 'Hit & Run'-Transformation primärer BRK-Zellen beitragen. (uni-regensburg.de)
It appears that the 34-kDa gene product encoded within open reading frame 6 (E4orf6) contributes to oncogenic transformation by modulating the function and stability of the tumor suppressor protein p53. (uni-regensburg.de)

4. The histone acetyltransferase activity of PCAF cooperates with the brahma/SWI2-related protein BRG-1 in the activation of the enhancer A of the MHC class I promoter. (nih.gov)
8. Stimulation of CREB binding protein nucleosomal histone acetyltransferase activity by a class of transcriptional activators. (nih.gov)
BACKGROUND The histone methyltransferase (HMT) family includes histone lysine methyltransferases (HKMTs) and histone/protein arginine methyltransferases (PRMTs). (cancerindex.org)

Altered expression of cyclins, such as overproduction of cyclins, particularly cyclin E, or production of alternative cyclin E proteins, as well as deranged appearance of mitotic cyclins during the cell cycle, are indicative of the presence of carcinoma. (everypatent.com)
There are 61335 CYCLIN domains in 41443 proteins in SMART's nrdb database. (embl-heidelberg.de)
Taxonomic distribution of proteins containing CYCLIN domain. (embl-heidelberg.de)
The complete taxonomic breakdown of all proteins with CYCLIN domain is also avaliable . (embl-heidelberg.de)
Click on the protein counts, or double click on taxonomic names to display all proteins containing CYCLIN domain in the selected taxonomic class. (embl-heidelberg.de)
The cyclin box fold: protein recognition in cell-cycle and transcription control. (embl-heidelberg.de)

C-terminal-binding protein interacting protein (CtIP) is a binding partner with CtBP, which contribute to transcription repression and cell cycle regulation, and which have a role in the cellular response to DNA damage. (wikipedia.org)
We have been interested in the role of phosphorylation of SR proteins in splicing regulation. (nature.com)

1986) Persistence of DNA synthesis arrest sites in the presence of T4 DNA polymerase and T4 gene 32, 44, 45 and 62 DNA polymerase accessory proteins, Nucl. (nih.gov)

Like HPV E7 and SV40 large T antigen, E1A proteins can form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). (bdbiosciences.com)
This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. (nih.gov)
Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. (nih.gov)

HN - 2006(1981) BX - Actin-Capping Proteins MH - Actin Depolymerizing Factors UI - D051339 MN - D5.750.78.730.212 MN - D12.776.220.525.212 MS - A family of low MOLECULAR WEIGHT actin-binding proteins found throughout eukaryotes. (nih.gov)

Anatomy16

Organisms29

Diseases11

Chemicals and Drugs89

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes67

Information Science3

Encodes7

MRNA6

Region of adenovirus1

Kinase9

TFIIB4

Binds5

Interacts3

Highly conserved3

Adenoviral2

Amino acids1

Multifunctional2

Assays4

Oncoproteins4

P30012

Herpes simpl3

Cell cycle progression3

Oncogenes3

Peptides2

Bind4

MRNAs3

Retinoblastoma tumour suppressor protein1

Apoptosis2

Mediate3

Genes6

Interaction4

Differentiation2

Regulate2

Gene expression4

Subunit1

Comprise2

Serotypes1

Helper virus1

Capsid4

Actin-related protein2

Nuclear5

Polypeptide3

Regulates3

Recognizes2

Kinases2

Rep782

Genome3

Receptor1

Viral replication1

Motif4

Modulate1

Terminus1

Human8

Degradation2

Signal transduc1

Zinc2

Mechanisms2

Biol1

Transformation3

Histone3

Cyclin6

Regulation2

Polymerase1

Complex3

Molecular1