Regulation of satellite cells stem satellite cells in human skeletal muscle. Medical search. Frequent questions

ENGLISH ABSTRACT: Introduction: Adult mammalian tissue regeneration recruits progenitor stem cells. (sun.ac.za)
Fibroblast Growth Factors (FGFs) are fundamental in embryonic development but also in adult skeletal muscle regeneration from injury or pathology. (sun.ac.za)
Understanding the role of specific members of this growth factor family could assist in improving the understanding of their influence on the regeneration sequence in skeletal muscle. (sun.ac.za)
Cellular and molecular regulation of muscle regeneration. (scielo.br)
Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
Skeletal muscle growth and regeneration involves the activity of resident adult stem cells, namely satellite cells (SC). (sdbonline.org)
Collectively, this study provides the first evidence that transient levels of the evolutionarily conserved factor RACK1 are critical for adult SC activation and proper skeletal muscle regeneration, favoring the efficient progression of SC from a committed to a fully differentiated state. (sdbonline.org)
In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
Our current running hypothesis is that the positive growth factors produced by hESCs trigger injured muscle to initiate and maintain regeneration, the TGF-beta inhibitors produced by hESCs reduce the TGF-beta signaling, and the combination assures the robust regeneration of muscle. (ca.gov)
Preliminary data indicates the effects on regeneration of old muscle look very promising. (ca.gov)
Specifically, our data suggest that several other ligands of MAPK pathway secreted by hESCs are likely to enhance and rejuvenate the regeneration of old muscle tissue. (ca.gov)
We have submitted the several manuscripts on topics of enhanced tissue regeneration and we are preparing the manuscript that identifies hESC-based novel strategies for restoring high regenerative capacity to old muscle. (ca.gov)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
There are shared interests with Musculoskeletal Tissue Engineering (MTE) in the investigation of skeletal muscle regeneration. (nih.gov)
Grant applications that focus on the use of scaffolds and muscle stem cells for skeletal muscle regeneration may be assigned to MTE. (nih.gov)
Applications that focus on in situ repair and regeneration of diseased skeletal muscle using skeletal muscle satellite or stem cells may be assigned to SMEP. (nih.gov)
1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)
Skeletal muscle satellite tv for pc cells (SCs) are stem cells chargeable for muscle improvement and regeneration. (korilog.com)
Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but a lack of mechanistic understanding of its relationship and tissue regeneration limits progress in this field. (ijbs.com)
We investigated the hypothesis that specific energy levels of LIPUS mediates skeletal muscle regeneration by modulating the inflammatory microenvironment. (ijbs.com)
LIPUS at intensity of 60mW/cm 2 could significantly promoted skeletal muscle regeneration through shifting macrophage phenotype from M1 to M2. (ijbs.com)
Macrophages later arrive at the tissue to remove cellular debris, and satellite cells (SCs) are activated and proliferate to induce myofiber regeneration. (biomedcentral.com)
Mesenchymal stromal cell spheroids in sulfated alginate enhance muscle regeneration. (ucdavis.edu)

Most studies investigating satellite cells in vitro use already activated satellite cells, called myoblasts. (sun.ac.za)
Finally, FGF6 and FGF2, both individually and sequentially, were used to treat quiescent myoblasts to determine their involvement in activation and proliferation with the use of cell cycle analysis and mRNA assessment of ki67, p21, myf5, and MyoD. (sun.ac.za)
This serum-free and grain-derived-nutrient-free medium promoted the proliferation of bovine myoblasts, the main cell source for cultured beef. (nature.com)
We hypothesized that by selecting optimal animal cells and using their CM to culture animal myoblasts, the cells could proliferate without the need for animal sera. (nature.com)
Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. (zen-bio.com)
ZenBio offers human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes. (zen-bio.com)
Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. (zen-bio.com)
Skeletal myoblasts are available from both male and female donors. (zen-bio.com)
Specially formulated media are available for maintenance and differentiation of our adult skeletal myoblasts. (zen-bio.com)
Satellite cells ( myoblasts ) are a type of stem cells that are still largely undifferentiated and are located in the vicinity of muscle fibers. (zxc.wiki)
We also demonstrate that the transcriptional regulator PRDM16 (PRD1-BF1-RIZ1 homologous domain containing 16) controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. (bioseek.eu)
Conversely, ectopic expression of PRDM16 in myoblasts induces their differentiation into brown fat cells. (bioseek.eu)

There is a longstanding belief that a given nucleus controls a defined volume of cytoplasm, so when a muscle grows (hypertrophy) or shrinks (atrophy), the number of myonuclei change accordingly. (frontiersin.org)
Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
This assumption long seemed valid, with many researchers reporting the presence of disintegrating nuclei in muscle tissue during atrophy induced by inactivity, injury or paralysis. (robert-gorter.info)
Muscles get damaged during extreme exercise, and often have to weather changes in food availability and other environmental factors that lead to atrophy. (robert-gorter.info)
Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)
Physical activity is known to prevent muscle atrophy, improve or boost metabolic activity, improve respiratory function, and positively impact muscles regarding strength, endurance, and stability. (apeironzoh.com)
It does this through various methods including stimulating the proliferation satellite cells (generating new muscle fibres) and inhibiting muscle atrophy - both of which are essential requirements for the increase of skeletal muscle mass (Fujita et al, 2007). (monarchhumanperformance.com)

The use of mesenchymal stem cells (MSCs) as a regenerative therapy holds the promise of regrowing injured vasculature, helping DM patients live healthier and longer lives. (thno.org)

Methods: Isolated satellite cells from human muscle biopsies were expanded in vitro creating primary human myoblast (PHM) clones. (sun.ac.za)
In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)
Cultured meat is produced by culturing/proliferating animal muscle cells in vitro. (nature.com)
We have chosen to work on human precursors, either in vitro or in vivo, in xenograft models that we have developed in successive European networks. (institut-myologie.org)
Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)

Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
We determined the developmental and functional status of both skeletal muscle progenitor cells as well as adult human satellite cells. (ca.gov)
A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. (ca.gov)

miR-133 suppresses Prdm16 expression in skeletal muscle stem cells (satellite cells), which controls myogenic vs. brown adipogenic lineage determination in these cells. (wikipedia.org)
Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. (biomedcentral.com)
Our team has demonstrated some important differences between man and mouse, in the regulation of the proliferation of myogenic precursors. (institut-myologie.org)
Evaluate myogenic ability and functional differences of embryonic, fetal, juvenile and adult MuSCs in fusion, proliferation, engraftment and repopulation assays in mouse models of muscle disease. (ca.gov)
The pro-angiogenic approaches also seem to be pro-myogenic and could resolve the age-related decline in satellite cell (SC) quantity seen in mdx models through expansion of the SC juxtavascular niche. (biomedcentral.com)
Here we show by in vivo fate mapping that brown, but not white, fat cells arise from precursors that express Myf5, a gene previously thought to be expressed only in the myogenic lineage. (bioseek.eu)

The myogenesis course of is flawed in DM1, which is intently related to progressive muscle weak spot and losing. (korilog.com)
MicroRNA-1/206(miRNA-1/206) is specifically expressed in skeletal muscle and play a critical role in myogenesis. (biomedcentral.com)
We have developed a comprehensive profile of human myogenesis using single cell sequencing. (ca.gov)
We will perform single cell RNA sequencing (scRNA-seq) of embryonic, fetal, juvenile and adult muscle stem cells (MuSCs) and identify unique gene signatures and cell states across human myogenesis. (ca.gov)

Cell Metabolism 2013;17(2):210-24. (scielo.br)
regulation of skeletal muscle energy and substrate metabolism including mitochondrial function. (nih.gov)
Liver kinase B1 (LKB1) plays pivotal roles in cellular processes such as apoptosis, metabolism, and cell cycle regulation. (omicsdi.org)
The size of an MND has a maximum that results from the ratio of sarcoplasm and cell nucleus, which is determined in a muscle cell by the requirements of an optimal metabolism. (zxc.wiki)
BAT may thus play a much more important role in human metabolism than was previously appreciated. (bioseek.eu)

Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
In the next reporting period we will re-confirm that the levels of candidate proteins from the 500 antibody array actually are very highly produced by hESCs and that the signals from these proteins are perceived by regenerating muscle cells. (ca.gov)
In 2010 beginning of 2011, we have approached the identification and characterization of the proteins that are produced by hESCs and have the rejuvenating and pro-regenerative activity on adult muscle. (ca.gov)
Biochemical and molecular biological research on skeletal muscle-specific proteins. (nih.gov)
Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. (rockland.com)
Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. (rockland.com)
There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
In addition, cytokines released in the process of myonecrosis recruit inflammatory cells, which release inflammatory cytokines to activate fibroblasts that lay down extracellular matrix proteins and lead to fibrosis[ 12 ]. (biomedcentral.com)
This decreases risk for various chronic diseases by eliminating damaging proteins, improving strength, and increasing muscle mass. (apeironzoh.com)
The cell membrane is really a viscous fluid-like matter which includes several lipids, cholesterol, and inserted proteins. (globaltechbiz.com)
Here are focal adhesion kinase (FAK) is activated, the set a chain of reactions in the sequence which the synthesis of muscle protein (e.g., contractile proteins such as actin and myosin , cytoskeletal proteins and enzymes of the citric acid cycle stimulate). (zxc.wiki)
The problem with building muscle is that, due to the synthesis of muscle protein, cytoskeletal proteins (supporting proteins) are also synthesized, which are supposed to protect the cell from mechanical overload. (zxc.wiki)

Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
Skeletal muscle regenerative capacity later diminishes with advancing age and decreasing numbers of SCs, and muscle tissue is steadily replaced by adipose and connective tissues[ 13 ]. (biomedcentral.com)

Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)

Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)
As well, many older individuals suffer from sarcopenia, a protracted muscle wasting disorder that typically begins after the age of 50 and involves a loss of about 1% of muscle mass per year ( Woo, 2017 ). (frontiersin.org)
skeletal muscle biology of sarcopenia and cachexia. (nih.gov)
There are shared interests with Aging Systems and Geriatrics (ASG) in the investigation of sarcopenia and skeletal muscle function. (nih.gov)
This work could provide a cell therapy for muscle disorders including muscular dystrophies or sarcopenia. (ca.gov)
A large majority of California residents are affected or will be affected with a muscle disease in their lifetime including severe muscular dystrophies, aging related muscle loss called sarcopenia or muscle weakness. (ca.gov)

We are developing a target product profile for human satellite cells derived from human pluripotent stem cells which will be used to use muscle stem cells in personalized medicine for patients with devastating muscle wasting or degenerative diseases. (ca.gov)
Here, we present a protocol for the maintenance and differentiation of human pluripotent stem cells into renal organoids. (bvsalud.org)

For example, in the human genome there are three known miR-133 genes: miR-133a-1, miR-133a-2 and miR-133b found on chromosomes 18, 20 and 6 respectively. (wikipedia.org)
Duchenne muscular dystrophy (DMD), the most common and severe form of muscular dystrophy, is caused by mutations in the dystrophin gene, the largest gene identified in the human genome. (biomedcentral.com)
It's been suggested that miRNAs regulate up to 50% of all mRNAs in the human genome. (genengnews.com)

Firstly, such modeling methods give an insight into how cell parts function under stress. (globaltechbiz.com)

MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
PRDM16 controls a brown fat/skeletal muscle switch. (bioseek.eu)
Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation. (bioseek.eu)
Finally, Prdm16-deficient brown fat displays an abnormal morphology, reduced thermogenic gene expression and elevated expression of muscle-specific genes. (bioseek.eu)

A beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion in mice. (omicsdi.org)
To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions. (omicsdi.org)
The results support the concept that fuel excess can drive obesity and diabetes via hyperinsulinaemia, and that an islet beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion. (omicsdi.org)
The epidemic of obesity, closely associated with increases in diabetes, hypertension, hyperlipidemia, cancer and other disorders, has propelled a major interest in adipose cells and tissues. (bioseek.eu)
Adipose tissues contain two distinct types of fat cells, white and brown. (bioseek.eu)
Until quite recently, brown adipose tissue (BAT) was thought to be of metabolic importance only in smaller mammals and infant humans. (bioseek.eu)
The manipulation of fat stores is an obvious therapeutic dream, but disruption of the normal differentiation or development of white adipose tissues (WAT) causes ectopic lipid storage and severe pathology (lipodystrophy) in both humans and experimental animals. (bioseek.eu)

miR-133 enhances myoblast proliferation by repressing serum response factor (SRF) mIR-133 suppresses SP1 expression In rats, miR-133b is expressed in retinal dopaminergicamacrine cell, and this expression is significantly increased during early stage during retinal degeneration. (wikipedia.org)
The supernatant, containing the RL34 cell-secreted growth factors, was used as the conditioned medium (CM). This CM, with CVE added as a nutrient source, was applied to primary bovine myoblast cultures. (nature.com)
Immortalized Canine Dystrophic Myoblast Cell Lines for Development of Peptide-Conjugated Splice-Switching Oligonucleotides. (institut-myologie.org)
Human skeletal myoblast are differentiated using Skeletal Muscle Differentiation Medium (cat# SKM-D) for at least 6 days. (zen-bio.com)

BMP2 controls bone cell determination by inducing miRNAs that target muscle genes but mainly by down-regulating multiple miRNAs that constitute an osteogenic program, thereby releasing from inhibition pathway components required for cell lineage commitment establish a mechanism for BMP morphogens to selectively induce a tissue-specific phenotype and suppress alternative lineages. (wikipedia.org)
We are culturing human fat cells (adipocytes) and tumour cells together, to find out how adipocytes alter tumour cell phenotype and response to therapy. (otago.ac.nz)

After acute injury in muscle of both mouse and the fruit fly Drosophila melanogaster (used as alternative in vivo model) this study found that RACK1 accumulated in regenerating fibers while it declined with the progression of repair process. (sdbonline.org)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
The concepts of her Cancer research study are interwoven with issues in Bone marrow failure, FANCA, Stem cell, Bone marrow and In vivo. (research.com)
The comprehensive functional tests and histological analysis in vivo indirectly and directly proved the effectiveness of LIPUS for muscle recovery. (ijbs.com)
Depletion of macrophages in vivo eliminated the therapeutic effects of LIPUS, indicating that improvement in muscle function was the result of M2-shifted macrophage polarization. (ijbs.com)
We will identify unique cell surface markers on in vivo derived MuSCs that distinguish PAX7-positive cells from other muscle cells and across cell states. (ca.gov)
Importantly, HDAC inhibitors block invasion and selectively eradicate LAM cells in vivo in zebrafish xenografts. (bvsalud.org)

These and other data argue against the current interpretation of the myonuclear domain hypothesis and suggest that once a nucleus has been acquired by a muscle fiber it persists. (frontiersin.org)
This strongly suggests that once a nucleus has been acquired by a muscle fiber, it belongs to the muscle syncytium - probably for life. (robert-gorter.info)

RACK1 was expressed transiently in the skeletal muscle of post-natal mice, being abundant in the early phase of muscle growth and almost disappearing in adult mature fibers. (sdbonline.org)
Until recently, scientists thought this meant that nuclei - the cell control centers that build and maintain muscle fibers - are also lost to sloth. (robert-gorter.info)
Two independent studies - one in rodents and the other in insects - have demonstrated that nuclei are not lost from atrophying muscle fibers, and even remain after muscle death has been initiated. (robert-gorter.info)
3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
When adapting muscles through muscle building, a distinction can be made between muscle hypertrophy , the thickening of existing muscle fibers, and muscle hyperplasia , the formation of new muscle fibers, the existence of which is controversial in humans. (zxc.wiki)
Muscle fibers are the constituent parts of a muscle. (zxc.wiki)

Thrown for a loop: fibro-adipogenic progenitors in skeletal muscle fibrosis. (ucdavis.edu)

Most of the known, partly still popular, theories, such as the hypothesis of increased blood circulation, the muscle hypoxia hypothesis and the ATP deficiency hypothesis (the ATP level in the cell remains constant), have been refuted. (zxc.wiki)

Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. (scielo.br)
Alignment, cross linking, and beyond: a collagen architect's guide to the skeletal muscle extracellular matrix. (ucdavis.edu)

Primary satellite cells can be harvested from muscle tissue to investigate or even use as potential therapeutic application. (sun.ac.za)
In conclusion, using clones that were distinctly different as assessed by the comparative index, this thesis illuminates that the two FGF family members investigated, act on cell cycle in different ways, thus would influence their utilization in experimental or therapeutic applications. (sun.ac.za)
Rapidly expanding technological innovations have led to a resurgence of interest in defining the factors that enable regenerative healing, and in coupling these factors to human therapeutic interventions. (frontiersin.org)
Additionally, our data in progress suggest that muscle and brain age by similar molecular mechanisms and thus, therapeutic strategies for rejuvenating muscle repair might be applicable to the restoration of neurogenesis in aged brain. (ca.gov)
Therapeutic and preventive interventions as they relate to skeletal muscle function are included, as are studies of the biochemistry and molecular biology of skeletal muscle and injuries, and diseases of muscle. (nih.gov)
Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)
Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
Its impact on disease paves the way for a deeper understanding of epigenetic regulation and novel therapeutic strategies. (bvsalud.org)

As a consequence of lower MEF2C binding on their enhancer region, the transcription of miR-1 and miR-133a is reduced, leading to decreased levels of their mature forms in muscle, after insulin treatment. (wikipedia.org)
Altered activation of PI3K and SREBP-1c may explain the defective regulation of miR-1 and miR-133a expression in response to insulin in muscle of type 2 diabetic patients. (wikipedia.org)
ATGL in beta cells regulates insulin secretion via the production of signalling MAGs. (omicsdi.org)
Skeletal muscle is an important site of insulin-stimulated glucose disposal and often the site of insulin resistance in obesity. (zen-bio.com)

miRNAs targeting MSTN are not implicated in posttranscriptional regulation of MSTN . (blogspot.com)
Moreover, miRNAs play a modulatory role in the development and growth of skeletal muscles [ 22 ]. (biomedcentral.com)
Three miRNAs, miRNA-1, −133 and −206, are specifically expressed in muscle and are considered to be myomiRs [ 23 , 24 ]. (biomedcentral.com)

This study further examined the time course for elevated muscle protein synthesis by examining its rate at 36 hrs following a training session. (researchgate.net)
IGF-1 is a growth hormone (GH) that is predominantly produced at a local level within skeletal muscle (in addition to the liver) with its key role being protein synthesis. (monarchhumanperformance.com)
Therefore, it works two-fold in the up-regulation of protein synthesis as well as inhibiting protein degradation. (monarchhumanperformance.com)

GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)

In this paper, we discuss the current available information about the role that different phenotypes of macrophages plays after injury and during the remodelling phase in different tissue types, with particular attention to the skeletal muscle. (hindawi.com)
Indeed, several human diseases are characterized by attenuated repair responses and imbalances in the inflammatory response with increased number of infiltrating macrophages [ 3 - 5 ]. (hindawi.com)
Alternatively activated M2 macrophages comprise cells exposed to low concentrations of M-CSF in the presence of IL-4, IL-13, or IL-10. (hindawi.com)

The Mackenzie Cancer Research Group has been investigating the contribution of stromal cells and the tumour microenvironment to human tumour biology since the inception of our research group in 1998. (otago.ac.nz)
Nature Reviews Molecular Cell Biology 2012;13(2):127-33. (scielo.br)
The Skeletal Muscle Biology and Exercise Physiology [SMEP] study section reviews applications concerned with molecular, cellular, physiological and integrative studies of normal and altered skeletal muscle function and processes that range from molecular genetics, to structure-function relationships, to integrative and functional studies on human mobility and exercise, and health. (nih.gov)
Heart, bone and even placenta are built on these networks of cells," says Lawrence Schwartz, Professor of Biology at the University of Massachusetts. (robert-gorter.info)
As such, the findings have important implications beyond understanding muscle biology. (robert-gorter.info)
Hiroyuki Koike is an Associate Professor in the Department of Medical Biochemistry, Tokyo Medical and Dental University, specializing in stem cell biology and developmental biology. (tmd.ac.jp)
Her primary areas of investigation include Cell biology, Immunology, Genetic enhancement, Haematopoiesis and Virology. (research.com)
Her Cell biology research is multidisciplinary, relying on both Dendritic cell, Interferon gamma, Interleukin 12, Bone marrow and Transplantation. (research.com)
The Immunology study combines topics in areas such as Progenitor cell and Molecular biology. (research.com)
Her studies deal with areas such as Cellular differentiation, CD40, Antigen-presenting cell, Dendritic cell and Interleukin 12 as well as Cell biology. (research.com)
Her biological study spans a wide range of topics, including Progenitor cell, CD34, Endothelial stem cell and Molecular biology. (research.com)
Genetic enhancement, Cell biology, Haematopoiesis, Viral vector and Transduction are her primary areas of study. (research.com)
Her Cell biology research is multidisciplinary, incorporating elements of Gene transfer, Transgene, Dose dependence and Immune system. (research.com)
The study incorporates disciplines such as Cell culture, Progenitor cell, CD34, Molecular biology and Transduction in addition to Haematopoiesis. (research.com)
She combines subjects such as Myeloid, Kidney disease and Cell biology with her study of Viral vector. (research.com)
The most prominent example is the changes in cell biology and morphology when it evolves from a healthy to a cancerous state [1, 3]. (globaltechbiz.com)

We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
Her research in Genetic enhancement intersects with topics in Hematopoietic stem cell transplantation, Cancer research, Wiskott-Aldrich syndrome and Prospective cohort study. (research.com)
She interconnects Cell, Progenitor cell, Protein domain, Enhancer and Hematopoietic stem cell in the investigation of issues within Transduction. (research.com)
Successful engraftment of gene-corrected hematopoietic stem cells in non-conditioned patients with Fanconi anemia. (research.com)
Anne Galy mainly focuses on Genetic enhancement, Haematopoiesis, Viral vector, Cancer research and Hematopoietic stem cell transplantation. (research.com)

Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)

The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)

Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
Grant applications that focus on rehabilitation interventions to improve muscle function, increase muscle mass, or identify muscles responsible to functional decline may be assigned to MRS. Applications that focus on molecular and cellular mechanisms of muscle function and related animal models may be assigned to SMEP. (nih.gov)
We have a strong expertise on human cellular models (this includes the immortalization facility that we have initiated) - and xenotransplantation (this includes several immunodeficient mouse models and grafting procedures). (institut-myologie.org)
Mostly, there is a finite amount of time that cells can survive and cumulative damage incurred from life choices can increase or decrease the speed of cellular damage. (apeironzoh.com)
Cytoskeleton, U-93631 which is a network of interconnected filaments of different types, connects the cell membrane with underlying sub-cellular components. (globaltechbiz.com)
The nucleus is the largest organelle among sub-cellular components, demonstrating solid-elastic behavior [4], and it is typically stiffer than the cell itself [5]. (globaltechbiz.com)

The anti-invasive effects of HDAC inhibitors are independent of genotype, while selective cell death is mTORC1-dependent and mediated by apoptosis. (bvsalud.org)

Dual luciferase assay and Western-blot results demonstrated that SFRP1 was a target of miR-1/206 in porcine iliac endothelial cells. (biomedcentral.com)
Engineered multi-functional, pro-angiogenic collagen-based scaffolds loaded with endothelial cells promote large deep burn wound healing. (ucdavis.edu)

There is a general agreement that hypertrophy is accompanied by the addition of new nuclei from stem cells to help the muscles meet the enhanced synthetic demands of a larger cell. (frontiersin.org)
Irradiation treatment, which eliminates satellite cells from these muscles, prevents the compensatory hypertrophy in these types of experiments. (arvojournals.org)
The mechanical metabolic pathway is activated by microtraumas and is of greater importance with regard to hypertrophy, since, in addition to an increase in the protein biosynthesis rate , it leads to a fusion of satellite cells with the muscle cell, whereby the total amount of DNA in the cell increases and thus the hypertrophy potential increases. (zxc.wiki)
This increases the total amount of DNA in the muscle cell and thus - due to new MNDs - the hypertrophy potential. (zxc.wiki)
The exact, extremely complex mechanisms of hypertrophy are not yet fully understood, but thanks to new technologies such as magnetic resonance imaging , which can take three-dimensional images of individual muscles, and dual x-ray absorptiometry , hypertrophy has been researched in more detail in recent years. (zxc.wiki)

MTF2 is highly conserved, and as an accessory subunit of PRC2, it has important roles in embryonic stem cell self-renewal and differentiation, development, and cancer progression. (bvsalud.org)

Microtraumas are the smallest injuries to the cell, triggered by supra-threshold mechanical stress and, to a much lesser extent, oxidative stress. (zxc.wiki)

Associate Professor Margaret Currie is interested in how the tumour microenvironment affects tumour growth, tumour cell metastasis and tumour response to therapy. (otago.ac.nz)
The local tumour microenvironment is the milieu within which the tumour develops, and includes tumour blood vessels, other cells types (e.g. immune cells, fibroblasts, adipocytes), soluble growth factors and signalling molecules. (otago.ac.nz)
All the various components of the tumour microenvironment can influence tumour growth and spread and, conversely, tumour cells can influence the local tumour microenvironment. (otago.ac.nz)
When activated by injury, satellite cells reenter the cell cycle and proliferate in response to extracellular growth factors ( Relaix & Zammit, 2012 Relaix F, Zammit PS. (scielo.br)
We confirmed that typical growth factor signaling was in fact occurring in muscle cells exposed to hESC produced factors, and that hESCs produce a TGF-beta antagonist. (ca.gov)
This fits with our recently published work showing that young muscle regenerates well from strong growth factor signaling and low TGF-beta signals while old muscle regenerated poorly due to weak growth factor signaling and high TGF-beta signaling. (ca.gov)
These residual 'myonuclei' allow more and faster growth when muscles are retrained - suggesting that we can "bank" muscle growth potential in our teens to prevent frailty in old age. (robert-gorter.info)
Muscle growth is accompanied by the addition of new nuclei from stem cells to help meet the enhanced synthetic demands of larger muscle cells," explains Schwartz. (robert-gorter.info)
During adolescence muscle growth is enhanced by hormones, nutrition and a robust pool of stem cells, making it an ideal period for individuals to "bank" myonuclei that could be drawn upon to remain active in old age. (robert-gorter.info)
The latent dedifferentiation capacity of newt limb muscles is unleashed by a combination of metamorphosis and body growth. (rockland.com)
Here, we developed a novel medium containing nutrients extracted from microalga and cell-secreted growth factors. (nature.com)
Animal cells are generally cultured in basal media supplemented with fetal bovine serum (FBS) which provides the various factors essential for cell growth. (nature.com)
The culture supernatant of animal cells, also known as 'conditioned medium' (CM), contains various factors secreted by the cells themselves, such as growth factors and cytokines. (nature.com)

However, since 2009 Associate Professor Currie's interests have expanded to include the contribution to tumour progression made by tumour infiltrating immune cell populations, breast cancer stem cell-like populations, and obesity-related factors. (otago.ac.nz)
He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
Immune cells that infiltrate lesions are important for atherosclerosis progression and immunotherapies. (biomedcentral.com)
Introduction Cell mechanics has proved to be a widely used label-free biomarker to discern phenotypes, detect pathologies and more importantly, monitor existence or progression of a disease [1C3]. (globaltechbiz.com)
Secondly, they can improve our understanding of the adjustments that occur during disease progression which, in turn, might uncover reasons for corresponding alterations occurring in cell mechanics [10, 11]. (globaltechbiz.com)

Muscle injuries can, therefore, be broadly classified as either traumatic (acute) or overuse (chronic) injuries. (bestbulksmsonline.com)
Acute injuries are usually the result of a single traumatic event and cause a macro-trauma to the muscle. (bestbulksmsonline.com)
Muscle injury is approached in a comprehensive way and includes descriptors such as acute, overuse, direct and indirect injury. (bestbulksmsonline.com)
A mutation in desmin makes skeletal muscle less vulnerable to acute muscle damage after eccentric loading in rats. (ucdavis.edu)

They have proinflammatory functions: they produce effector molecules (reactive oxygen and nitrogen intermediates) and inflammatory cytokines (IL-1 β , TNF α , and IL-6) and participate as inducer and effector cells in polarized Th1 responses. (hindawi.com)
But modern cell-type-specific dyes and genetic markers have shown that the dying nuclei other researchers had detected were in fact inflammatory and other cells recruited to atrophic muscle. (robert-gorter.info)

The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. (biomedcentral.com)

The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
The Wnt signaling pathway is involved in the control of cell proliferation and differentiation during skeletal muscle development. (biomedcentral.com)

Physical activity induces autophagy in the brain and muscles. (apeironzoh.com)

Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)

Dr. Bosinger is defining the molecular mechanisms of virus pathogenesis through application of next-gen RNA sequencing to studies of HIV and SARS-CoV-2 infection in non-human primates. (emory.edu)

Secondly, the quiescent state is an integral part of stem cell regulation, therefore choosing the right protocol for inducing quiescence is important. (sun.ac.za)
Unlike FGF2, individual treatment with FGF6 was adequate to activate the quiescent PHMs and aid their re-entry into cell cycle with consistency in all three PHM clones by upregulating ki67 expression. (sun.ac.za)
The inverse sequential treatment order did not demonstrate any significant effect on both activation and proliferation of the quiescent cells. (sun.ac.za)
In mice, sustained YAP1 hyperactivity in activated, but not quiescent, satellite cells induces ERMS with high penetrance and short latency. (elsevierpure.com)

Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)

Her Haematopoiesis research integrates issues from Regulation of gene expression, Mutant and Transduction. (research.com)
Each cell nucleus has a certain "sphere of influence" which it controls through its gene expression. (zxc.wiki)

Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)
Biomimetic hydrogel culture of LAM cells is found to recapitulate the molecular and phenotypic characteristics of human disease more faithfully than culture on plastic. (bvsalud.org)
These changes take place on the molecular level impacting properties of specific the different parts of cell inner structure, but resulting in alterations in mechanical properties of the complete cell ultimately. (globaltechbiz.com)

ROCK 1 and 2 affect the spatial architecture of 3D spheroids derived from human corneal stromal fibroblasts in different manners. (rockland.com)

Together, these data suggest that CXCR4 plays an important role in donor cell engraftment. (biomedcentral.com)
Therefore, we sought to translate these results to a clinically relevant canine-to-canine allogeneic transplant model for Duchenne muscular dystrophy (DMD) and determine if CXCR4 is important for donor cell engraftment. (biomedcentral.com)
We show that CXCR4 expressing cells are important for donor muscle cell engraftment, yet FACS sorted CXCR4-positive cells display decreased engraftment efficiency. (biomedcentral.com)
We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)

A common treatment for motility disorders of the extraocular muscles (EOMs) is a resection procedure in which there is surgical shortening of the muscle. (arvojournals.org)

At 8 weeks of age, these mice were injected with tamoxifen to induce deletion of beta cell-specific Atgl (also known as Pnpla2), and the mice were fed an ND or HFD. (omicsdi.org)
In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)
We reported that LIPUS therapy at intensity of 60mW/cm 2 exhibited the most significant differences in functional recovery of contusion-injured muscle in mice. (ijbs.com)
Mice develop tumors resembling those seen in humans, including expression of high levels of miR-21. (genengnews.com)

Anatomy55

Organisms18

Diseases16

Chemicals and Drugs80

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes54

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena8

Technology, Industry, Agriculture1

Information Science6

Health Care2

Regeneration26

Myoblasts12

Atrophy7

Mesenchymal stem1

Vitro7

Progenitor cells4

Myogenic6

Myogenesis4

Metabolism5

Proteins15

Regenerative capacity4

Primary skeletal1

Sarcopenia6

Pluripotent Stem2

Genome3

Methods1

Prdm164

Adipose7

Myoblast4

Phenotype2

Vivo7

Acquired by a2

Fibers7

Fibrosis1

Hypothesis1

Extracellular matrix2

Therapeutic9

Insulin4

MiRNAs3

Protein synthesis3

Mitochondrial dysfunction1

Macrophages3

Biology16

Hematopoietic6

Progenitors1

Pathways1

Cellular6

Apoptosis1

Endothelial cells2

Hypertrophy5

Self-renewal1

Oxidative1

Growth13

Progression5

Acute4

Inflammatory2

Significantly1

Proliferation and differentiation2

Autophagy1

Adult muscle stem cells1

Mechanisms1

Quiescent4

Individual myofibers1

Gene-expression2

Molecular3

Stromal1

Donor4

Disorders1

Mice5