• RNA-based evolution is a theory that posits that RNA is not merely an intermediate between Watson and Crick model of the DNA molecule and proteins, but rather a far more dynamic and independent role-player in determining phenotype. (wikipedia.org)
  • By regulating the transcription in DNA sequences, the stability of RNA, and the capability of messenger RNA to be translated, RNA processing events allow for a diverse array of proteins to be synthesized from a single gene. (wikipedia.org)
  • In accordance with the central dogma of molecular biology, RNA passes information between the DNA of a genome and the proteins expressed within an organism. (wikipedia.org)
  • Research within the past decade has shown that strands of RNA are not merely transcribed from regions of DNA and translated into proteins. (wikipedia.org)
  • Currently RNA acts by forming proteins. (wikipedia.org)
  • Spermatogenesis produces haploid sperm capable of penetrating the oocyte, whereas oogenesis produces differentiated oocytes that are stockpiled with maternal nutrients, proteins, and mRNAs, and have outer layers that protect the embryo and enable fertilization. (elifesciences.org)
  • Ribonucleic acids (RNAs) carry as messenger-RNAs (mRNAs) genetic information from DNA to cellular protein factories, where they are translated into proteins. (mpg.de)
  • But they also have important regulatory functions: Small noncoding RNAs (miRNAs) influence mRNA stability and are able to switch off genes by stalling their translation into proteins. (mpg.de)
  • As far as humans are concerned, the argonaute protein Ago-2 is the key cellular binding partner of miRNAs: The Ago-miRNA complex binds to mRNA and impedes their translation into proteins - either by blocking the translation process or by initiating RNA decomposition. (mpg.de)
  • The pleiotropic effects of both proteins are perfect examples of the im​portance of RNA decay and translation in maintaining correct cell functioning. (ens-lyon.fr)
  • Y-Complex Proteins Show RNA-De. (uni-marburg.de)
  • In this project, I will employ biochemical, cell, systems biology and computational approaches to study the relevance of post-transcriptional regulators such as RNA-binding proteins and microRNAs in DDR. (europa.eu)
  • Quantitative proteomics will be applied to identify the differentially mRNA-bound proteins after the exposure of human cultured cells to ionizing radiation. (europa.eu)
  • This will provide a global insight into the dynamics of RNAs and proteins during DDR. (europa.eu)
  • The proteins are RNA-binding and have roles in the regulation of splicing, export and stability of mRNA. (antibodiesinc.com)
  • Mechanically, m6A can be recognized by "reader" proteins or can directly modify RNA conformation, and it widely affects gene expression by mediating RNA stability, translation, splicing and export. (scienceopen.com)
  • RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
  • Together these proteins shorten the end of the mRNAs, preparing the molecules for degradation. (elifesciences.org)
  • Dr. Saravana Murthy, VP of Research at JCRI, explains the patented translational molecular effect, "During the growth phase of cells, the messenger molecules (mRNAs) responsible for producing Histone Proteins are closely controlled to maintain cell division, stability, and survival. (businesswire.com)
  • In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
  • Both proteins mediate protein-protein and protein-RNA interactions for cooperative RNA-binding during spliceosome assembly. (cipsm.de)
  • They are RNA-BINDING PROTEINS involved in mRNA processing. (bvsalud.org)
  • ribosomal proteins and histones have higher fractions of optimal codons while transcriptional regulators and mRNAs predicted to be localized to synapses have lower fractions of optimal codons. (escholarship.org)
  • 2021) Principles of mRNA targeting and regulation via the Arabidopsis m6A-binding proteins ECT2 and ECT3. (oeaw.ac.at)
  • 2021) The YTHDF proteins ECT2 and ECT3 bind largely overlapping target sets and influence target mRNA abundance, not alternative polyadenylation. (oeaw.ac.at)
  • Brodersen P, Arribas-Hernández L, ..., Nodine MD (2021) The Arabidopsis m6A-binding proteins ECT2 and ECT3 bind largely overlapping mRNA target sets and influence target mRNA abundance, not alternative polyadenylation. (oeaw.ac.at)
  • Although, the number of omic techniques is ever expanding, the most developed omics technologies are high throughput DNA sequencing, transcriptomics (focused on gene expression), epigenomics (focused on epigenetic regulation of gene expression), proteomics (focused on large sets of proteins, the proteome) and metabolomics (focused on large sets of metabolites, the metabolome). (who.int)
  • Cellular RNAs are regulated at multiple stages, including transcription, RNA maturation and degradation. (nature.com)
  • Short tails of repetitive nucleic acids are often added to the ends of RNA molecules in order to prevent degradation, effectively increasing the number of RNA strands able to be translated into protein. (wikipedia.org)
  • A transcriptional arrest leads to a strong change in localization and dynamics of YmcA, YlbF and YaaT, supporting their involvement in global RNA degradation. (uni-marburg.de)
  • Here we show that m6A is selectively recognized by the human YTH domain family 2 (YTHDF2) protein to regulate mRNA degradation. (scienceopen.com)
  • Such interactions generally result in a decrease in protein synthesis, either by stimulating mRNA degradation or by inhibiting translation. (biomedcentral.com)
  • The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (nih.gov)
  • A limited study had indicated that in Sinorhizobium meliloti the YbeY ortholog regulates the accumulation of sRNAs as well as the target mRNAs, raising the possibility that YbeY may play a previously unrecognized role in bacterial sRNA regulation. (biomedcentral.com)
  • This is especially interesting, because over the past years there have been controversial discussions as to whether or not small noncoding RNAs occur in the nucleus. (mpg.de)
  • microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (nih.gov)
  • Importin 8 interacts with Ago and miRNA and is necessary for the binding of the AgomiRNA-complex to a variety of mRNA targets: In the cytoplasm - i.e. the intracellular space outside the nucleus - it recruits the complex to its target, allowing for efficient and specific gene-silencing. (mpg.de)
  • Non-coding RNA (ncRNA) such as microRNA (miRNA) and long non-coding RNA (lncRNA) regulates the translation and/or stability of multiple mRNA targets either directly or by modulation of signal transduction pathways. (frontiersin.org)
  • Consequently, motif quality is a major determinant of mRNA stability for Puf3 targets in vivo and can be used for the prediction of mRNA targets. (elifesciences.org)
  • Decreased expression of the RNA-binding protein, Pumilio, stabilizes predicted neural mRNA targets and presence of a 3'UTR PRE is sufficient to trigger mRNA decay in the nervous system. (escholarship.org)
  • Conceptual parallels exist between bacterial and eukaryotic small-RNA (sRNA) pathways, yet relatively little is known about which protein may recognize and recruit bacterial sRNAs to interact with targets. (biomedcentral.com)
  • In bacteria, numerous sRNAs that range in size from ~50-300 nucleotides in length act on independently transcribed mRNA targets. (biomedcentral.com)
  • The most extensively characterized class of bacterial sRNAs are the trans -encoded sRNAs that are encoded distant from the genes for their mRNA targets and that typically have only limited complementarity (10-30 nt) [ 2 ]. (biomedcentral.com)
  • Trans- encoded sRNAs form imperfect base-pairing interactions with complementary sequences in their mRNA targets, which are often located at or near ribosome binding sites (RBS), but can also be located upstream of the translation start site as well as deep in the coding regions (CDS) [ 2 ]. (biomedcentral.com)
  • miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (nih.gov)
  • Besides classical gene regulation, miRNAs have emerged as post-transcriptional regulators of angiogenesis. (plos.org)
  • miRNAs comprise a large group of endogenous non-coding RNAs that can block mRNA translation or negatively regulate mRNA stability and thereby play a central role in regulating gene expression [10] , [11] . (plos.org)
  • DEFB1 expression after transfection with two micro RNAs (miRNAs), hsa-miR-186-5p and hsa-miR-340-5p, provided evidence that DEFB1 expression could be modulated by these miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. (cdc.gov)
  • Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)
  • Molecules of RNA have also been shown to effectively self-replicate, catalyze basic reactions, and store heritable information. (wikipedia.org)
  • Single-stranded RNA molecules can single handedly fold into complex structures. (wikipedia.org)
  • Phenotype may also be determined by the number of RNA molecules, as more RNA transcripts lead to a greater expression of protein. (wikipedia.org)
  • During mammalian liver regeneration RNA molecules of growth factors increase in number due to the addition of signaling tails. (wikipedia.org)
  • Silencing of RNA occurs when double stranded RNA molecules are processed by a series of enzymatic reactions, resulting in RNA fragments that degrade complementary RNA sequences. (wikipedia.org)
  • Angiogenesis is a balanced process controlled by pro- and anti-angiogenic molecules of which the regulation is not fully understood. (plos.org)
  • In addition, I will characterize the transcriptome-wide response of existing RNA molecules as well as the newly synthesized transcripts. (europa.eu)
  • Transfer RNA (tRNA) molecules contain various post-transcriptional modifications that are crucial for tRNA stability, translation efficiency, and fidelity. (mdpi.com)
  • Puf3 allows Ccr4-Not to select specific mRNAs from a mixture of molecules. (elifesciences.org)
  • Since RNA processing is heritable, it is subject to natural selection suggested by Darwin and contributes to the evolution and diversity of most eukaryotic organisms. (wikipedia.org)
  • The Ccr4-Not complex removes mRNA poly(A) tails to regulate eukaryotic mRNA stability and translation. (elifesciences.org)
  • EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
  • In the past, we have been working on two cellular factors, the translation initiation factor INT6/EIF3E and the RNA helicase UPF1, that act at the crossroads of RNA translation and decay. (ens-lyon.fr)
  • We established recently how the viral protein Tax is able to interact and alter some functions of INT6/EIF3E and UPF1, especially in Nonsense Mediated mRNA Decay (NMD). (ens-lyon.fr)
  • However, the initial rate of decay was similar in control and TNF-treated cells, suggesting that reduced expression in TNF-treated cells was not due to decreased stability of IL-2 mRNA. (biomedcentral.com)
  • N6-Methyladenosine (m 6 A), a predominant internal modification of RNA in mammalian cells, has been recognized as having a vital role in mRNA stability, export, translation, splicing, and decay [ 4 ]. (hindawi.com)
  • The C-terminal domain of YTHDF2 selectively binds to m6A-containing mRNA whereas the N-terminal domain is responsible for the localization of the YTHDF2-mRNA complex to cellular RNA decay sites. (scienceopen.com)
  • Although candidate studies have revealed important mechanisms of post-transcriptional regulation of gene expression via mRNA decay, there is a lack of global information on mRNA decay during development. (escholarship.org)
  • TU-decay technology allows measurement of genome-wide mRNA decay in intact Drosophila embryos, across all tissues and specifically in the nervous system. (escholarship.org)
  • In Drosophila whole embryos, codon optimality is correlated with mRNA decay, while in the nervous system, codon-mediated effects on mRNA decay are attenuated. (escholarship.org)
  • This work demonstrates a dynamic post-transcriptional program including mRNA decay allows fine-tuning of gene expression during neural development. (escholarship.org)
  • Specific gene-silencing is involved in the development of cancer and plays an essential role in gene regulation. (mpg.de)
  • Possibly it is involved in gene regulation, too. (mpg.de)
  • Intriguingly, some of the top-ranked transcripts are noncoding splicing isoforms, which imply diverse gene regulation mechanisms. (nature.com)
  • mRNA half-lives have been determined, for example, by blockage of transcription followed by transcriptional profiling 5 . (nature.com)
  • Processing of RNA influences protein expression by managing the transcription of DNA sequences, the stability of RNA, and the translation of messenger RNA. (wikipedia.org)
  • The MZT marks the transfer of control of development from the mother to the zygote as maternal mRNAs are degraded, transcription from the zygotic genome begins, and embryonic development becomes dependent on zygotic gene products ( Tadros and Lipshitz, 2009 ). (elifesciences.org)
  • transcription regulation, and complex RNA design. (edu.au)
  • mRNAs that are predicted to be translated within axon growth cones or dendrites have long half-lives while mRNAs encoding transcription factors that regulate neurogenesis have short half-lives. (escholarship.org)
  • Ribonucleoprotein complex immunoprecipitation and biotinylated RNA pull-down assays demonstrated that PARP-14 forms a complex with the mRNA-destabilizing protein tristetraprolin (TTP) and a conserved adenylate-uridylate-rich element in the TF mRNA 3′ untranslated region. (imperial.ac.uk)
  • RNA dependent RNA polymerase (RdRp) sequence of RaTG13 shows that it is 100% similar to that of bat corona virus BtCoV/4991 and 98.7-98.9% similar to SARS-CoV-2 RdRp 2 . (preprints.org)
  • The formation of these complex structure gives reason to suspect that early life could have formed by RNA. (wikipedia.org)
  • While there are a lot of studies concerning miRNA processing, the target mRNA recognition and binding by the Ago-miRNA-complex is only poorly understood", says Gunter Meister, the head of the research group "RNA biology" at the Max Planck Institute. (mpg.de)
  • It is essential to exhaustively interrogate complex mRNA isoforms of protein-coding genes with an unified data resource. (nature.com)
  • In order to understand the relative importance of kinetics and stability, we now report rapid rates of protein/RNA complex assembly and dissociation for two IRE-RNAs with IRP1, and quantitatively different metal ion response kinetics that coincide with the different iron responses in vivo. (cuny.edu)
  • IRP1/IRE-RNA complex has a much shorter life-time than the eIF4F/IRE-RNA complex, which suggests that both rate of assembly and stability of the complexes are important, and that allows this regulatory system to respond rapidly to change in cellular iron. (cuny.edu)
  • To better apprehend how a RNA can act on gene expression regulation as part of a RiboNucleoProtein complex (RNP), we are also interested in specific RNP formations and processings. (ens-lyon.fr)
  • It is thought that the Y-complex acts as an accessory factor in RNase Y regulation but might also have independent functions. (uni-marburg.de)
  • Gene expression is extensively regulated at the levels of mRNA stability, localization and translation. (nih.gov)
  • Mechanistically, MS-444 disrupted HuR cytoplasmic trafficking and released ARE-mRNAs for localization to P-bodies, but did not affect total HuR expression levels. (oncotarget.com)
  • Right here, we investigate the systems where TGF- adversely regulates TRIII messenger RNA (mRNA) manifestation in the transcriptional level in both breasts and ovarian tumor model systems. (accessibletech4all.org)
  • Overexpression of the RNA-binding protein HuR (ELAVL1) occurs during colon tumorigenesis and is abnormally present within the cytoplasm, where it post-transcriptionally regulates genes through its interaction with 3'UTR AU-rich elements (AREs). (oncotarget.com)
  • PARN is an extensively characterized exonuclease with deadenylation activity that controls mRNA stability in part and therefore regulates expression of a large number of genes. (jci.org)
  • In this section, we comprehensively analyzed the expression levels of fourteen m 6 A RNA methylation regulators and clinical factors in patients with HCC from the ICGC (International Cancer Genome Consortium, https://icgc.org/ ), Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/ ), and TCGA (The Cancer Genome Atlas, http://cancergenome.nih.gov/ ) databases. (hindawi.com)
  • We have applied a multipronged approach of loss-of-function studies, genome-wide mRNA and sRNA expression profiling, pathway analysis, target prediction, literature mining and network analysis to unravel YbeY-dependent molecular responses of E. coli exposed to hydroxyurea (HU). (biomedcentral.com)
  • The genome of RaTG13 (MN996532.1) was sequenced from the RNA of a bat faecal swab collected in 2013 from Yunnan, China, however the exact location is not mentioned. (preprints.org)
  • RNA-seq experiments generate reads derived not only from mature RNA transcripts but also from pre-mRNA. (nature.com)
  • Therefore, from an evolutionary standpoint, a mutation within the DNA bases results in an alteration of the RNA transcripts, which in turn leads to a direct difference in phenotype. (wikipedia.org)
  • By degrading transcripts, a lower amount of protein products are translated and the phenotype is altered by yet another RNA processing event. (wikipedia.org)
  • Therefore, information regarding reference annotated mRNA transcripts of respective human protein-coding genes would be beneficial for biomedical researches and pathological sequence variation analyses. (nature.com)
  • In order to investigate representative mRNA transcript isoforms to be utilized as transcriptome analysis references, we utilized GTEx data to establish a top-ranked transcript isoform expression data resource for human protein-coding genes. (nature.com)
  • This allows the cell to switch networks of related genes off together without affecting the mRNAs it still needs. (elifesciences.org)
  • Observed during CRC tumorigenesis is loss of post-transcriptional regulation of tumor-promoting genes such as COX-2, TNFα and VEGF. (oncotarget.com)
  • Individuals with biallelic PARN mutations and PARN -depleted cells exhibited reduced RNA levels for several key genes that are associated with telomere biology, specifically TERC , DKC1 , RTEL1 , and TERF1 . (jci.org)
  • Pansensitive and panresistant genes to 21 NCCN-recommended drugs with concordant mRNA and protein expression were identified. (cdc.gov)
  • With considerable accumulation of RNA-Seq transcriptome data, we have extended our understanding about protein-coding gene transcript compositions. (nature.com)
  • Due to the development of RNA sequencing technology, the distribution pattern of m6A in the transcriptome has been uncovered. (scienceopen.com)
  • Rapid kinetics of iron responsive element (IRE) RNA/iron regulatory pr" by Mateen A. Khan, Jia Ma et al. (cuny.edu)
  • In order to further understand IRE-mRNA regulation in terms of kinetics and stability, eIF4F kinetics with FRT IRE-RNA were determined. (cuny.edu)
  • Here we present a computational approach called exon-intron split analysis (EISA) that measures changes in mature RNA and pre-mRNA reads across different experimental conditions to quantify transcriptional and post-transcriptional regulation of gene expression. (nature.com)
  • Furthermore, changes in post-transcriptional regulation can be predicted from differences between exonic and intronic changes. (nature.com)
  • However, decoding functional RNA-regulatory features remains a limitation to understanding post-transcriptional regulation in vivo. (nih.gov)
  • Post-transcriptional regulation is essential for temporal and spatial control of protein expression. (escholarship.org)
  • Here, we reconstitute accelerated and selective deadenylation of RNAs containing AU-rich elements (AREs) and Pumilio-response elements (PREs). (elifesciences.org)
  • Shortening or removal of mRNA poly(A) tails (deadenylation) represses gene expression in eukaryotes. (elifesciences.org)
  • This deadenylation deficiency caused an early DNA damage response in terms of nuclear p53 regulation, cell-cycle arrest, and reduced cell viability upon UV treatment. (jci.org)
  • Thus, alternative RNA splicing events allow differential phenotypes, regardless of the identity of the coding DNA sequence. (wikipedia.org)
  • Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)
  • We implemented RESA to identify sequence elements modulating mRNA stability during zebrafish embryogenesis. (nih.gov)
  • The Tra gene, determinant of sex, in male flies becomes truncated as splicing events fail to remove a stop codon that controls the length of the RNA molecule. (wikipedia.org)
  • The methods (RNA or codon optimization to increase mRNA stability transport and expression) had applications in both DNA and viral vaccine vectors and also in gene therapy protocols. (cancer.gov)
  • Splicing is the process by which non-coding regions of RNA are removed. (wikipedia.org)
  • However, for an immediate and effective response, global post-transcriptional control of mRNA stability, splicing and translation may be crucial during "immediate-to-early" DDR. (europa.eu)
  • In general, these protocols aim to enrich for mature mRNA by selection of polyadenylated RNA or by depletion of ribosomal RNA. (nature.com)
  • Here, we developed RNA-element selection assay (RESA), a method that selects RNA elements on the basis of their activity in vivo and uses high-throughput sequencing to provide a quantitative measurement of their regulatory functions at near-nucleotide resolution. (nih.gov)
  • The Node will bring together expertise in high containment, in vitro cultivation (SARS-CoV-2, influenza, other), animal infection models, analysis of viral RNA elements, testing of antiviral efficacy and assessment of innate and adaptive antibody and cellular immune responses. (edu.au)
  • Regulation of RNA stability and translation are two key steps of cellular homeostasis. (ens-lyon.fr)
  • Besides their canonical roles in translation, tRNAs also originate tRNA-derived small RNAs (tsRNAs), a class of small non-coding RNAs with regulatory functions ranging from translation regulation to gene expression control and cellular stress response. (mdpi.com)
  • Improved wound healing due to cardiac overexpression RNA m5C modification's emerging role in Cardiovascular Diseases. (alliedacademies.org)
  • Most of the m 6 A RNA methylation regulators were confirmed to be differentially expressed among groups stratified by clinical characteristics and tissues. (hindawi.com)
  • We identified an m 6 A RNA methylation regulator-based signature (including METTL3, YTHDC2, and YTHDF2) that could effectively stratify a high-risk subset of these patients by univariate and LASSO Cox regression, and receiver operating characteristic (ROC) analysis indicated that the signature had a powerful predictive ability. (hindawi.com)
  • In summary, we demonstrated the vital role of m 6 A RNA methylation regulators in the initial presentation and progression of HCC and constructed a nomogram which would predict the clinical outcome and provide a basis for individualized therapy. (hindawi.com)
  • Our results also revealed the presence of tail-length-independent mechanisms that maintained translation despite tail-length shortening during oocyte maturation, and prevented essentially all translation of bicoid and several other mRNAs before egg activation. (elifesciences.org)
  • A prerequisite for rapid adaptations is efficient RNA turnover, with endonuclease RNase Y playing a crucial role in mRNA stability as well as in maturation. (uni-marburg.de)
  • In a study based on exon arrays, probes mapping to introns were used to investigate pre-mRNA dynamics 23 . (nature.com)
  • The proposed project aims to identify the dynamics of RNA-protein interactions during DNA damage response (DDR) using high-throughput "omics" approaches. (europa.eu)
  • Searches for candidate cis-regulatory elements identified enrichment of the Pumilio recognition element (PRE) in mRNAs encoding regulators of neurogenesis. (escholarship.org)
  • Finally, we used the versatile RESA platform to map candidate protein-RNA interactions in vivo (RESA-CLIP). (nih.gov)
  • YbeY extensively modulates Hfq-dependent and independent sRNA-mRNA interactions. (biomedcentral.com)
  • RNAs are critical in gene expression and that gene expression depends on mRNA, rRNA, and tRNA. (wikipedia.org)
  • An extensive repertoire of modifications is known to underlie the versatile coding, structural and catalytic functions of RNA, but it remains largely uncharted territory. (scienceopen.com)
  • We apply EISA to 17 diverse data sets to show that most intronic reads arise from nuclear RNA and changes in intronic read counts accurately predict changes in transcriptional activity. (nature.com)
  • Rather RNA has retained some of its former independence from DNA and is subject to a network of processing events that alter the protein expression from that bounded by just the genomic DNA. (wikipedia.org)
  • N6-methyladenosine (m6A) is the most abundant inner RNA modification in eukaryotes. (scienceopen.com)
  • Small regulatory RNAs (sRNAs) play key roles in modulating gene expression in both prokaryotes and eukaryotes. (biomedcentral.com)
  • In others the stop signal is retained within the final RNA molecule and a functional Tra protein is produced resulting in the female phenotype. (wikipedia.org)
  • In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
  • When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
  • In this regard, we plan to further address the role of INT6/EIF3E in the regulation of mRNA translation and how this function impacts genetic instability and cancer development (project 3 and 4) . (ens-lyon.fr)
  • The regulations under REACH have been estimated to estimates of possible in vivo responses based on in vitro screens. (cdc.gov)
  • Total RNA was extracted and probed for IL-2 mRNA by ribonuclease protection assay. (biomedcentral.com)
  • Secondary, mRNA-seq analysis identified Gadd45 as a potential transcriptional target of dGLYAT, as depletion of dGLYAT decreased Gadd45 mRNA level. (sdbonline.org)
  • This was related to an increase in TF messenger RNA (mRNA) stability. (imperial.ac.uk)
  • HCC tissues from patients had higher levels of PVRL1 messenger RNA and protein than nontumorous tissues. (stanford.edu)
  • knockdown of Pvrl1 reduced cell-surface levels of PVR but not levels of Pvr messenger RNA. (stanford.edu)
  • Although biochemical studies indicate that N(6)-methyladenosine (m(6)A) is the most prevalent internal modification in messenger RNA, an in-depth study of its distribution and functions has been impeded by a lack of robust analytical methods. (scienceopen.com)
  • Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide, and N6-methyladenosine (m 6 A) is a predominant internal modification of RNA in various cancers. (hindawi.com)
  • Although RNA-seq mostly generates reads that map to exons, it also captures less abundant intronic sequences 6 . (nature.com)
  • The mRNAs can carry short sequences of genetic letters that can trigger their own destruction. (elifesciences.org)
  • Although macrophage TF expression is known to be regulated at the RNA level, very little is known about the mechanisms involved. (imperial.ac.uk)