• In this study, we observed that the expression of pseudouridine (Ψ) synthase 10 is increased in aged hematopoietic stem and progenitor cells (HSPC) and enforced protein of Ψ synthase 10 (PUS10) recapitulates the phenotype of aged HSC, which is not achieved by its Ψ synthase activity. (haematologica.org)
  • Interleukin 13: novel role in direct regulation of proliferation and differentiation of primitive hematopoietic progenitor cells. (ox.ac.uk)
  • In this study we investigated for the first time the potential role of IL-13 in the regulation of the growth of hematopoietic progenitor cells. (ox.ac.uk)
  • Effective expansion of umbilical cord blood hematopoietic stem/progenitor cells by regulation of microencapsulated osteoblasts under hypoxic condition. (ox.ac.uk)
  • The expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood (UCB) with the support of microencapsulated osteoblasts under hypoxia environment was investigated. (ox.ac.uk)
  • Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
  • In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
  • The hematopoietic tissue contains cells with long-term and short-term regeneration capacities and committed multipotent, oligopotent, and unipotent progenitors. (wikipedia.org)
  • Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
  • Recently we have developed an experimental and analytical method, termed massively parallel single-cell RNA-Seq (MARS-Seq), for unbiased classification of individual cells from their native context and successfully applied it for characterization of immune and hematopoietic progenitors. (europa.eu)
  • Exciting preliminary result show that indeed our approach is starting to uncover the complexity of hematopoietic progenitors and the regulatory circuits driving hematopoietic decisions. (europa.eu)
  • We will pursue the following aims: (i) Generate a refined model of hematopoiesis by comprehensive single-cell RNA-Seq profiling of hematopoietic progenitors, (ii) validate the predicted model by in vivo functional developmental assays and then (iii) test candidate transcription and chromatin factors uncovered by our model for their role in controlling progression towards various lineages using single-cell measurements combined with CRISPR screens. (europa.eu)
  • Notch signals are required for in vitro but not in vivo maintenance of human hematopoietic stem cells and delay the appearance of multipotent progenitors. (sunnybrook.ca)
  • Research in the Paralkar Lab spans the spectrum from human patient sample studies and mouse models to cutting-edge molecular biology tools, high-throughput sequencing approaches, and novel computational algorithms, all with the goal of gaining insight into how the transcription of coding genes and noncoding ribosomal DNA genes is regulated in hematopoietic stem cells, myeloid progenitors, and in leukemia. (upenn.edu)
  • Aged hematopoietic stem cells (HSC) exhibit compromised reconstitution capacity and differentiation-bias towards myeloid lineage, however, the molecular mechanism behind it remains not fully understood. (haematologica.org)
  • This study reveals effects of cell cycle progression on the expression of lineage specific genes in precursor cells, and suggests that hematopoietic stress changes the balance of renewal and differentiation in these homeostatic cells. (nih.gov)
  • and regulation of stem cell differentiation. (nih.gov)
  • These changes result in impaired differentiation of hematopoietic stem cells, which leads to CN-AML. (medlineplus.gov)
  • Mezigdomide was designed to target Ikaros and Aiolos, key transcription factors in hematopoietic cell development and differentiation, the authors continued. (medpagetoday.com)
  • We also found an unexpectedly dynamic pattern of transcript isoform regulation, suggesting a critical regulatory role during HSC differentiation, and a cell cycle/DNA repair signature associated with multipotency in MPP2 cells. (lu.se)
  • Studies on the pathogenesis of bone marrow failure syndromes have helped to elucidate the regulation of cellular growth and differentiation in the hematopoietic environment. (medscape.com)
  • Bone marrow hematopoietic stem cells (HSCs) are vital for the maintenance of hematopoiesis. (irbbarcelona.org)
  • In inbred mice housed in gnotobiotic facilities, the top of the hematopoietic hierarchy is occupied by dormant HSCs, which reversibly exit quiescence during stress. (irbbarcelona.org)
  • Due to the scarcity of HSCs, technical challenges have limited their insights into the interplay between HSC metabolism and their transcriptional and epigenetic regulation. (irbbarcelona.org)
  • Hematopoietic stem cells (HSCs) emerge during development via an endothelial-to-hematopoietic transition from hemogenic endothelium of the dorsal aorta (DA). (cardiff.ac.uk)
  • Hematopoietic stem cells (HSCs) are the stem cells that give rise to other blood cells. (wikipedia.org)
  • In vertebrates, the very first definitive HSCs arise from the ventral endothelial wall of the embryonic aorta within the (midgestational) aorta-gonad-mesonephros region, through a process known as endothelial-to-hematopoietic transition. (wikipedia.org)
  • Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
  • In this study, we present integrated quantitative proteome, transcriptome, and methylome analyses of hematopoietic stem cells (HSCs) and four multipotent progenitor (MPP) populations. (lu.se)
  • The rapid advancement in understanding the biology of hematopoietic stem cells (HSCs) and cancer stem cells (CSCs) in recent years has renewed interest in investigating the role of stem cells in benzene -induced malignancy and bone marrow depression. (cdc.gov)
  • scRNA-seq on murine hematopoietic stem cells (HSC) and their progeny MPP1 separated the cells into 3 main clusters with distinct features: active, quiescent, and an un-characterized cluster. (nih.gov)
  • One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
  • Researchers at the Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) have now found a gene mechanism that is responsible for the aging of hematopoietic stem cells. (idw-online.de)
  • The eventual aging of hematopoietic stem cells is apparently already preprogrammed by their gene-driven growth in youth. (idw-online.de)
  • Cytomegalovirus reactivation is still a critical concern following allogeneic hematopoietic cell transplantation, and cellular immune reconstitution of cytomegalovirus-specific cytotoxic T-cells is necessary for the long-term control of cytomegalovirus reactivation after allogeneic hematopoietic cell transplantation. (nature.com)
  • Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
  • Here, we focus on understanding the contradictory regulation of drHSC self-renewal and multipotency: upon transplantation, drHSC self-renewal is induced, whereas their intrinsic lineage bias is preserved. (grantome.com)
  • These methods and results can help us to better understand immune reconstitution following hematopoietic cell transplantation, leading to future studies on the clinical application of adoptive T-cell therapies. (nature.com)
  • Direct and indirect antigen presentation lead to deletion of donor-specific T cells after in utero hematopoietic cell transplantation in mice. (ca.gov)
  • In utero hematopoietic cell transplantation (IUHCTx) is a promising method to induce donor-specific tolerance but the mechanisms of antigen presentation that educate host T cells and the relative importance of deletion vs. regulation in this setting are unknown. (ca.gov)
  • Studies indicate that early unrelated hematopoietic stem cell transplantation in both the infantile and late-onset forms is associated with at least short-term benefits on neurocognitive parameters, lifespan, and quality of life. (medscape.com)
  • We identify an evolutionarily conserved non-coding region (ECR) in the Hhex locus with the capacity to bind the hematopoietic-affiliated transcriptional regulators Gata2, SCL, Fli1, Pu.1 and Ets1/2. (cardiff.ac.uk)
  • Transcriptional regulation of voltage-gated sodium channels contributes to GM-CSF induced pain. (iasp-pain.org)
  • In vertebrates, the vast majority of hematopoiesis occurs in the bone marrow and is derived from a limited number of hematopoietic stem cells that are multipotent and capable of extensive self-renewal. (wikipedia.org)
  • Together, our study is expected to generate a revised and high-resolution hematopoietic model and decipher the regulatory networks that control hematopoiesis. (europa.eu)
  • The Paralkar Lab has identified that key hematopoietic and leukemic transcription factors bind to rDNA and regulate rRNA transcription, and we are interested in understanding how the binding of cell-type-specific transcription factors regulates the activity of Polymerase I and the transcription of rRNA in normal hematopoiesis, and how this regulation is co-opted in leukemia to drive abundant ribosome biogenesis. (upenn.edu)
  • I returned to Sweden after that and worked as a postdoc for one year before starting my own lab, initially focusing on human hematopoietic stem cell regulation in normal and malignant hematopoiesis. (lu.se)
  • Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)
  • The Hhex ECR was also sufficient to drive expression at additional blood sites including the yolk sac blood islands, fetal liver, vitelline and umbilical arteries and the adult bone marrow, suggesting a common mechanism for Hhex regulation throughout ontogenesis of the blood system. (cardiff.ac.uk)
  • Hematopoietic stem cells are found in the bone marrow of adults, especially in the pelvis, femur, and sternum. (wikipedia.org)
  • Another CFU, the colony-forming unit-spleen (CFU-S), was the basis of an in vivo clonal colony formation, which depends on the ability of infused bone marrow cells to give rise to clones of maturing hematopoietic cells in the spleens of irradiated mice after 8 to 12 days. (wikipedia.org)
  • Under the direction of Melissa Kacena, PhD , the Kacena Lab is exploring the interaction of the bone and hematopoietic systems, thereby potentially improving the treatment of metabolic bone disease, hematopoietic disorders and fracture healing. (iu.edu)
  • The hematopoietic stem cells in the bone marrow continuously ensure that the blood system is supplied with new cells throughout life and that in stressful conditions, such as infections, inflammations or bleeding, the production of the required blood cells can then be initiated immediately. (idw-online.de)
  • In this prospect, benzene metabolites formed through metabolism in the liver and bone marrow cause damage in hematopoietic cells via multiple mechanisms that, in addition to traditionally recognized chromosomal aberration and covalent binding, incorporate oxidative stress, alteration of gene expression, apoptosis, error-prone DNA repair, epigenetic regulation, and disruption of tumor surveillance. (cdc.gov)
  • The Thomas lab has a long-term interest in understanding the epigenetic regulation of embryonic development and in particular has characterised the function of histone acetyltransferases. (edu.au)
  • This study provides a comprehensive genome-wide resource for the functional exploration of molecular, cellular, and epigenetic regulation at the top of the hematopoietic hierarchy. (lu.se)
  • Exploring the evidence for epigenetic regulation of environmental influences on child health across generations. (cdc.gov)
  • Hematopoietic progenitor kinase 1 (HPK1) regulates stress responses, proliferation, and apoptosis in hematopoietic cells. (aacrjournals.org)
  • Hematopoietic stem cells give rise to different types of blood cells, in lines called myeloid and lymphoid. (wikipedia.org)
  • Hematopoietic stem cells constitute 1:10,000 of cells in myeloid tissue. (wikipedia.org)
  • Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)
  • These factors may regulate myeloid versus lymphoid balance with age, and can potentially mitigate the long-term deleterious effects of inflammation that lead to hematopoietic pathologies. (biorxiv.org)
  • We develop a computational model for the hematopoietic erythroid-myeloid lineage decision, which is determined by a genetic switch involving the genes PU.1 and GATA-1. (lu.se)
  • Citation: Chickarmane V, Enver T, Peterson C (2009) Computational Modeling of the Hematopoietic Erythroid-Myeloid Switch Reveals Insights into Cooperativity, Priming, and Irreversibility. (lu.se)
  • RNA splicing is a major nexus of gene expression regulation, shaping cellular identity during development, frequently altered in human cancers. (lu.se)
  • No significant alteration of hematopoietic homeostasis and HSC function is observed in young Pus10-/- mice, while aged Pus10-/- mice exhibit mild alteration of hematopoietic homeostasis and HSC function. (haematologica.org)
  • In this first renewal application of this award, we propose to pursue the regulation of self-renewal and lineage potential of the developmentally restricted hematopoietic stem cells (drHSCs) that we discovered in the current award period. (grantome.com)
  • self-renewal and multilineage potential - are established during development and maintained for life and we are excited to put these powerful tools to work to pioneer developmental hematopoietic fate decisions. (grantome.com)
  • Researchers at the Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) in Jena have been able to demonstrate that in mice, the growth factor Igf2bp2 controls hematopoietic stem cell function in young adulthood by activating stem cell metabolism and growth. (idw-online.de)
  • The experimental findings of the current study suggest that the activation of growth and metabolism in juvenile mice preprograms the subsequent loss of function of hematopoietic stem cells and inscribes this into the cell's memory. (idw-online.de)
  • Using this expertise, the scientists were able to identify a new subset of hematopoietic stem cells that exhibit particularly strong activity of Igf2bp2-dependent metabolism and growth in adolescent mice. (idw-online.de)
  • Little is known however about how rRNA transcription is regulated and fine-tuned across normal and malignant tissues, and whether this regulation can be targeted for leukemia treatment. (upenn.edu)
  • Human CD8 T cells generated in vitro from hematopoietic stem cells are functionally mature. (sunnybrook.ca)
  • In vitro studies showed the mezigdomide-induced degradation of the two factors enhanced cytotoxic effects in myeloma cells, including cells resistant to IMiDs lenalidomide (Revlimid) and pomalidomide (Pomalyst) and with cereblon down-regulation. (medpagetoday.com)
  • Heterogeneity within hematopoietic progenitor populations has considerably limited characterization and molecular understanding of lineage commitment in both health and disease. (europa.eu)
  • The very first hematopoietic stem cells during (mouse and human) embryonic development are found in aorta-gonad-mesonephros region and the vitelline and umbilical arteries. (wikipedia.org)
  • Whether the metabolic and dividing activity of hematopoietic stem cells during embryonic development or in adolescence already predetermines later aging of the cells had not been previously been reported and was therefore the subject of the current study. (idw-online.de)
  • citation needed] Hematopoietic stem cells can be identified or isolated by the use of flow cytometry where the combination of several different cell surface markers (particularly CD34) are used to separate the rare Hematopoietic stem cells from the surrounding blood cells. (wikipedia.org)
  • EPCs are enriched in the population coexpressing CD34, CD133, and KDR with a low or absent expression of pan-hematopoietic marker CD45 [ 1 ]. (hindawi.com)
  • As bioactive molecule, ceramide is involved in the regulation of many cellular processes. (elifesciences.org)
  • Hematopoietic stem cells (HSC) generate all of the blood cells throughout their life-span. (haematologica.org)
  • In shape, hematopoietic stem cells resemble lymphocytes. (wikipedia.org)
  • Since hematopoietic stem cells cannot be isolated as a pure population, it is not possible to identify them in a microscope. (wikipedia.org)
  • Lack of expression of lineage markers is used in combination with detection of several positive cell-surface markers to isolate hematopoietic stem cells. (wikipedia.org)
  • In addition, hematopoietic stem cells are characterised by their small size and low staining with vital dyes such as rhodamine 123 (rhodamine lo) or Hoechst 33342 (side population). (wikipedia.org)
  • Hematopoietic stem cells are essential to haematopoiesis, the formation of the cells within blood. (wikipedia.org)
  • Hematopoietic stem cells can replenish all blood cell types (i.e., are multipotent) and self-renew. (wikipedia.org)
  • A small number of Hematopoietic stem cells can expand to generate a very large number of daughter Hematopoietic stem cells. (wikipedia.org)
  • 00:39 police is these are hematopoietic stem cells. (yale.edu)
  • Polycythemia vera (PV) is a clonal disorder resulting from neoplastic transformation of hematopoietic stem cells, while secondary polycythemia (SP) is a disease characterized by increased absolute red blood cell mass caused by stimulation of red blood cell production. (nature.com)
  • However, as the organism ages, increased metabolic activity can also lead to functional exhaustion of hematopoietic stem cells. (idw-online.de)
  • We have shown that KAT6A (MOZ) is essential for the development of haematopoietic stem cells whereas KAT6B (QKF) has an essential role in adult neural stem cells. (edu.au)
  • rRNA transcription rates vary dramatically between different normal cell types in the hematopoietic tree, and leukemic cells have characteristic prominent nucleoli, indicating robust ribosome synthesis. (upenn.edu)
  • Research on vitamin D regulation of thymocyte selection, Th1 and Th17 cells, T-cell programed cell death, and T-regulatory (Treg) cells is summarized and integrated into model mechanisms. (frontiersin.org)
  • In a study published in the Molecular Cell journal, they explain what makes hematopoietic stem cells acquire malignant traits in cancer. (lu.se)
  • Later, I pursued my Ph.D. at Lund University, focusing on the regulation of hematopoietic stem cells using genetic mouse models under the guidance of Stefan Karlsson. (lu.se)
  • After completing my Ph.D., I joined Hannah Mikkolas' group at UCLA in Los Angeles, where I continued studying blood stem cell regulation, this time using human primary cells and establishing a disease model for leukemia. (lu.se)
  • Transitioning from a well-established field like hematopoietic stem cells to the relatively new field of lung biology required starting over in many aspects. (lu.se)
  • With Prof. Eva Hellström-Lindberg's group at the Karolinska Institute, Maciej Cieśla and coworkers discovered dynamic regulation of SF3B1 levels during the malignant transformation from MDS to leukemia. (lu.se)
  • The Igf2bp2-gene drives growth and metabolic activity at a young age but these activities contribute to the age-associated loss of hematopoietic stem cell function in later life. (idw-online.de)
  • Within the genome, many processes occur almost simultaneously so that the regulation of possibly chaotic access is tightly controlled. (birmingham.ac.uk)
  • Our methods and models may provide a platform for future medical advancements including a large-scale European collaborative project to discover a comprehensive human hematopoietic tree. (europa.eu)
  • After evaluating serologic data, transmission patterns, and regulations in the context of brucellosis in dogs as an underrecognized zoonosis, we concluded that brucellosis in dogs remains endemic to many parts of the world and will probably remain a threat to human health and animal welfare unless stronger intervention measures are implemented. (cdc.gov)
  • Therefore, the mechanisms which allow for the regulation of angiogenesis are of great importance. (hindawi.com)