Regulation of gene biological regulation mammalian embryonic. Medical search. Frequent questions

For improving our understanding of epigenetic regulation of gene expression: the biological mechanisms that affect how genetic information is variably expressed. (wraltechwire.com)
Recently, the importance of epigenetic regulation via microRNA in controlling stem cell activities has been recognized [31-37]. (buyresearchchemicalss.net)
Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. (nature.com)
The epigenetic regulation of spatiotemporal gene expression is crucial for human development. (researchgate.net)
Although this work provides important insights into how epigenetic regulation of developmental enhancers in NSCs encodes the complex organization of the mammalian cerebral cortex, much work is still needed to fully understand these biological processes and their implications for human disease. (indiana.edu)
Mammalian parental imprinting is a form of epigenetic regulation that causes genes to be expressed from only one chromosome homolog according to parent-of-origin 1 , 2 . (nature.com)

It has been reported that Sirtuin 6 (Sirt6), a member of the sirtuin family of NAD + -dependent protein deacetylases, is involved in embryonic stem cell differentiation. (biomedcentral.com)
Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)

However, most JmjC genes do not critically regulate development, as 10 members are viable and fertile with no obvious developmental defects. (sdbonline.org)
We show that the conditional depletion of the m 6 A reader protein Ythdf2 in mice causes lethality at late embryonic developmental stages, with embryos characterized by compromised neural development. (biomedcentral.com)
Background: Bivalent chromatin domains consisting of the activating histone 3 lysine 4 trimethylation (H3K4me3) and repressive histone 3 lysine 27 trimethylation (H3K27me3) histone modifications are enriched at developmental genes that are repressed in embryonic stem cells but active during differentiation. (researchgate.net)
Central to this is the transient increase in H3K4-trimethylation at developmental genes during G1, thereby creating a 'window of opportunity' for cell-fate specification. (researchgate.net)
They can diffuse from a source and penetrate to a target within tissues making them ideal candidates for developmental signals and an important model system to study the role of regulated transcription in fundamental biological processes. (uci.edu)
Chemistry, robotics, signal transduction, gene regulation, reverse genetics and basic cell, developmental and molecular biology are key to identifying new hormonal signaling pathways. (uci.edu)
While imprinting perturbations are widely associated with developmental abnormalities, the intricate regional interplay between imprinted genes makes interpreting the contribution of gene dosage effects to phenotypes a challenging task. (nature.com)

Consistently, expression of genes enriched in neural development pathways is significantly disturbed. (biomedcentral.com)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
The Wang lab uses genomics approaches to investigate regulatory pathways in mammalian stem cells. (northwestern.edu)
This revealed distinct classes of distal SMARCA4-associated elements characterized by activating and repressive chromatin signatures that were associated with tissue-specific up- or down-regulation of gene expression and relevant active/repressed biological pathways. (harvard.edu)
ToxTracker is a mammalian stem cell-based assay that monitors activation of specific cellular signalling pathways for detection of the biological reactivity of compounds. (europa.eu)
These processes require coordinated gene expression and are regulated by a large and diverse group of inter- and intracellular signaling pathways mediated by an equally diverse group of hormones and hormone receptors. (uci.edu)
The 5-HT neurotransmitter regulates important pathways of mammalian metabolism and is synthesized from the phenylalanine, tyrosine and tryptophan amino acids 5 . (bvsalud.org)

Compartmentalization of dJmj in nucleolus together with some of PcG may be necessary to de-repress the expression of genes required to cellular growth and proliferation in the following meiotic divisions. (sdbonline.org)
The multifunctional regulator nuclear factor erythroid 2-related factor (Nrf2) is considered not only as a cytoprotective factor regulating the expression of genes coding for anti-oxidant, anti-inflammatory and detoxifying proteins, but it is also a powerful modulator of species longevity. (springer.com)
Mammalian parental imprinting represents an exquisite form of epigenetic control regulating the parent-specific monoallelic expression of genes in clusters. (nature.com)

In addition, compounds will be analysed for their ability to induce hypoxia by assessing the expression of HIF1 alpha target genes using qPCR. (europa.eu)
Long-term efforts of this project will involve developing RAR-alpha selective antagonists, identifying the target genes of RAR-alpha, and determining their function in germ cell-Sertoli cell interactions. (columbia.edu)
These 'nuclear receptors' are ligand-modulated transcription factors that directly regulate the expression of target genes. (uci.edu)

DNA methylation, including the prevailing 5-methylcytosine (5mC) modification, is a fundamental epigenetic mechanism that regulates various biological functions, including cancer, neurodegeneration, and embryogenesis. (hku.hk)
My graduate and postdoctoral research has focused on investigating how the genome and epigenome regulates the dynamics of gene expression in viral and mammalian systems 8 . (confex.com)
Notably, using CUT&RUN and ATAC-seq assays, we discovered that IPMK co-localizes with BRG1 and regulates BRG1 localization as well as BRG1-mediated chromatin accessibility in a genome-wide manner (including promoter-TSS) in mouse embryonic stem cells. (sciety.org)
BRG1 occupancy is preferentially affected at promoters with bivalent chromatin modifications and its diminished occupancy regulates gene expression. (sciety.org)

Generation of molecularly defined null mutants revealed that loss of 8 out of 13 JmjC genes modify position effect variegation (PEV) phenotypes, consistent with their ascribed role in regulating chromatin organization. (sdbonline.org)
In the present review, the phenotypes of Met gene‑modified mice are summarized. (spandidos-publications.com)
From an engineering perspective, gene expression regulation can be viewed as the output of a network of complex chemical and physical processes, and understanding how these processes interact and integrate to govern cellular phenotypes has been a major focus of my graduate and postdoctoral research. (confex.com)
This is a list of variants' phenotypes submitted to ClinVar and linked to the RYR2 gene. (genebe.net)
Yet, the intricate form of epigenetic control over the parent-specific expression of multiple genes in an imprinted cluster poses difficulties when trying to decipher the relative contribution of changes in imprinted gene dosage to the resulting physiological phenotypes. (nature.com)

Mouse embryonic fibroblasts isolated from transgenic Oct4-GFP reporter mice with or without Sirt6 were used for reprogramming by Yamanaka factors. (biomedcentral.com)
The research interests of the Wolgemuth lab focus on understanding the genetic control of gametogenesis and embryogenesis using mouse models and gene targeting, transgenic, and molecular and cell biological approaches. (columbia.edu)
In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)

2 Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Barcelona, Spain. (nih.gov)
Redundant gene models were identified and merged based on the genomic coordinates, resulting in 5,571 standalone annotated lincRNA genes (See Methods and Materials, as well as the Additional File 1 for more details). (biomedcentral.com)
During my graduate studies, I used a systems biology approach to demonstrate that chromatin environments at different genomic loci decouple transcription factor mediated initiation of gene expression from subsequent gene activation 6 . (confex.com)
A global genomic transcriptional code associated with CNS-expressed genes. (nih.gov)
The authors show convincing data that IPMK interacts with SWI/SNF and modulates is genomic occupancy in embryonic stem (ES) cells to regulate gene expression. (sciety.org)
Cloning new vertebrate receptors offers the opportunity to use molecular genetic techniques, biochemistry and genomic technology to identify new hormones important in embryonic development and adult physiology. (uci.edu)

Monkeys have served as one of the most valuable models for understanding DNA methylation dynamics during early embryogenesis in human due to their similarities in genetics and early embryonic development 17 , 18 . (nature.com)

Among the factors thought to contribute to the greater success in cloning cattle are the relatively late embryonic genome activation speciﬁc for this species [16 -18] and the optimization of reproductive technologies, such as in vitro embryo production and embryo transfer, brought about by the cattle industry [19]. (sibi.org)

These microRNAs are expressed at high levels in several human embryonic stem cell (ESC) and human embryonal carcinoma cell (EC) lines [49, 50] and decrease upon differentiation into embryoid body (EB) for two weeks in culture [50] supporting their association with the stem cell state. (buyresearchchemicalss.net)
Furthermore, due to ethical and legal concerns, very limited techniques can be applied to human embryos to validate some of significant conclusions drawn from descriptive studies regarding human embryonic development. (nature.com)
Based on multiple RNA-Seq datasets, we systematically identified 300 human embryonic stem cell lincRNAs (hES lincRNAs). (biomedcentral.com)
As the first global profiling and annotating of human embryonic stem cell lincRNAs, this work aims to provide a valuable resource for both experimental biologists and bioinformaticians. (biomedcentral.com)
Combining a comprehensive collection of human embryonic stem cell RNA-Seq datasets with Human BodyMap 2, we validated that 295 previously annotated lincRNAs were expressed in multiple human embryonic stem cell samples and further identified five novel hES lincRNAs through de novo assembling. (biomedcentral.com)
Moreover, we surveyed and manually screened published hES RNA-Seq datasets in several public repositories, resulting in a list of 31 wild-type human embryonic stem cell samples. (biomedcentral.com)
And H3K4me3, H3K27me3 and H3K9ac are more reliable predictors than other histone modifications in human embryonic stem cells. (oncotarget.com)

We provide the first evidence that miRNAs naturally induce transcriptome-wide covariations and compare the relative importance of nuclear organization, transcriptional and post-transcriptional regulation in defining covariations. (nih.gov)
However, the direct linkage between IPMK and chromatin remodelers remains unclear, raising a question on how IPMK contributes to the transcriptional regulation in mammals. (sciety.org)
We showed that the BET family member BRDT is essential for proper chromatin remodeling and transcriptional regulation during meiotic prophase and also during spermiogenesis and that BRD2 is essential for embryonic survival and neural differentiation and function, in particular in the etiology of seizure susceptibility. (columbia.edu)
This is because chemical purification is guided simply by biological activity as measured by transcriptional regulation. (uci.edu)
Evolution of transcriptional regulation in cortical neural stem cells. (indiana.edu)

Our studies have demonstrated that several of the proteins encoded by cellular oncogenes function in fundamental aspects of gene regulation. (stanford.edu)
Synthetic biology has evolved over the years to the point where analogue gene switches have been assembled to create complex genetic circuits that can program cellular behaviour. (electropages.com)
encoded by Nfe2l2 gene) is a transcription factor responsible for the regulation of cellular redox balance and protective antioxidant and phase II detoxification responses in mammals [ 1 , 2 ]. (springer.com)
To study the cellular function of Ecd in mammalian cells, we generated Ecd(lox/lox) mouse embryonic fibroblast cells from Ecd floxed mouse embryos. (unmc.edu)
His Cell biology research is multidisciplinary, incorporating elements of Cellular differentiation, Transcription factor, Regulation of gene expression, Centrosome cycle and Centriole elongation. (research.com)
This includes gene, protein and metabolic networks, cellular architecture and intracellular dynamics, cell communication and motility, cell division and differentiation, tissue formation and organogenesis, tissue and organ functions, changes in population characteristics as a consequence of interaction of organisms with their physical environment, with individuals of their own species, and with organisms of other species. (nih.gov)
The dysfunction is associated with issues with apoptosis and normal cellular metabolic regulation, all regulated through OPA1. (thermofisher.com)
ToxTracker consists of a panel of six different mES GFP reporter cell lines representing four distinct biological responses that are associated with carcinogenesis, i.e. general cellular stress (biomarker gene: Btg2), DNA damage (biomarker genes: Bscl2 and Rtkn), oxidative stress (biomarker genes: Srxn1 and Blvrb) and protein damage (biomarker gene: Ddit3). (europa.eu)

The Symposium Proceedings addresses 21st Century Genetics: Genes at Work, and provides a current synthesis of genetic mechanisms and genome/chromosome biology. (cshlpress.com)
Biological systems are analogue in nature, are programmed by genetics, and are controlled by the flow of ions through protein membranes. (electropages.com)
Michael T. McManus focuses on Cell biology, Genetics, microRNA, Gene silencing and Molecular biology. (research.com)
His Gene study introduces a deeper knowledge of Genetics. (research.com)
The scientist's investigation covers issues in Cell biology, Gene, Computational biology, Immune system and Genetics. (research.com)

MicroRNAs (miRNA), short (17?24 nucleotides), non-coding RNAs that have been identified in various organisms including mammalian cells, are thought to play a key role in several biological processes [38-41]. (buyresearchchemicalss.net)

Sirt6 was previously reported to regulate many different biological processes like genome stability, glucose metabolism, and tumor suppression [ 18 ]. (biomedcentral.com)
Functional analysis showed that they were preferentially involved in several early-development related biological processes. (biomedcentral.com)
All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
In further study, we find that even though transcription factors and histone modifications offer similar effects on expression levels of genome-wide genes, the effects of transcription factors and histone modifications on predictive abilities are different for genes in independent biological processes. (oncotarget.com)
We have taken an integrated approach toward understanding the role of orphan steroid receptors in important biological processes. (uci.edu)
The main goal of my lab is to understand how genome-wide epigenetic modifications in NSCs and cortical neurons determine the organization and function of the cerebral cortex and the importance of these biological processes in human neurodevelopmental disorders. (indiana.edu)

Although the genome-wide DNA demethylation is believed to be a hallmark of mammalian embryogenesis, previous study also indicated that the somatic form of dnmt1 ( dnmt1s ) is actually expressed at each stage of pre-implantation embryos and plays a role in the maintenance of DNA imprinting 8 . (nature.com)
However, the role of SMARCA4 in tissue-specific gene regulation during embryogenesis remains poorly defined. (harvard.edu)

Single-cell RNA sequencing studies on gene co-expression patterns could yield important regulatory and functional insights, but have so far been limited by the confounding effects of differentiation and cell cycle. (nih.gov)
Histone modifications and chromatin-associated protein complexes are crucially involved in the control of gene expression, supervising cell fate decisions and differentiation. (researchgate.net)
Earlier studies [ 1 - 4 ] showed transcription factors (TFs) binding and histone modifications (HMs) were critical for gene expression, and the abnormities of TFs binding and HMs may affect the cell fate such as differentiation and apoptosis [ 5 ]. (oncotarget.com)
Genetic control of mammalian germ cell differentiation. (columbia.edu)

Exon 5 is expressed in embryonic heart, down-regulated and ceases express during postnatal development. (wikipedia.org)
Outline the importance of apoptosis during embryonic development and in the maintenance of appropriate cell number. (southampton.ac.uk)
We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
Prof. Eldad Tzahor of the Institute's Biological Regulation Department thought that part of the answer to the regeneration puzzle might lie in his area of expertise: embryonic development, especially of the heart. (eurekalert.org)
2) What are the requirements for DNA replication and transcription at the beginning of mammalian development? (nih.gov)
This, in turn, affected cell fate determination, highlighting the importance of understanding the role of 6mA in embryonic development. (hku.hk)
The Ecdysoneless (Ecd) protein is required for cell-autonomous roles in development and oogenesis in Drosophila, but the function of its evolutionarily conserved mammalian orthologs is not clear. (unmc.edu)
Regulation of mammalian Notch signaling and embryonic development by the protein O-glucosyltransferase Rumi. (nih.gov)
Biostatistics areas of interest include development of advanced statistical techniques and methodologies for design of biological experiments, collection and analysis of the data from those experiments and interpretation of, and inference from, the results. (nih.gov)
Of interest are development of computational and mathematical algorithms and tools, modeling techniques and approaches for understanding the complexity of biological systems, and utilization of big datasets and data science methods for model construction. (nih.gov)
The SMARCA4 (also known as BRG1 in humans) chromatin remodeling factor is critical for establishing lineage-specific chromatin states during early mammalian development. (harvard.edu)
Our results indicate a dual active/repressive function of SMARCA4 at distal regulatory sequences in vivo and support its role in tissue-specific gene regulation during embryonic development. (harvard.edu)
In contrast, enhancer silencing by PRDM16 reduces the expression of cell migration genes in NSCs, thereby controlling the final position of cortical neurons at later stages of development. (indiana.edu)
Several drugs cross the placental barrier and some have teratogenic effects on the fetus, which may cause dysfunction and disorders during embryonic development, particularly during the first three months of intrauterine life 2 . (bvsalud.org)

Her experiments reveal a strategy for how DNA repair proteins locate DNA lesions and demonstrate a biological role for DNA-mediated charge transfer. (wraltechwire.com)
We find that nuclear organization has the greatest impact, and that genes encoding for physically interacting proteins specifically tend to covary, suggesting importance for protein complex formation. (nih.gov)
Jumonji (JmjC) domain proteins (see Jarid2 ) influence gene expression and chromatin organization by way of histone demethylation, which provides a means to regulate the activity of genes across the genome. (sdbonline.org)
The major characteristics of Nrf2 are to some extent mimicked by Nrf2-dependent genes and their proteins including heme oxygenase-1 (HO-1), which besides removing toxic heme, produces biliverdin, iron ions and carbon monoxide. (springer.com)

RNA sequencing was measured to identify the differential expressed genes due to loss of Sirt6 in somatic and pluripotent cells. (biomedcentral.com)
Prior to SCNT, the somatic cell (differentiated) must be reprogramed to a similar state of a pluripotent embryonic cell (undifferentiated) before the nucleus is extracted and transferred. (sibi.org)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
Here we show that bivalent domains and chromosome architecture for bivalent genes are dynamically regulated during the cell cycle in human pluripotent cells. (researchgate.net)

Catenins form functional networks in the nucleus to regulate gene expression and determine cell fate. (bvsalud.org)
IMPK modulates SWI/SNF chromatin binding in particular at the transcription start sites of promoters with bivalent chromatin modifications in embryonic stem cells to regulate gene expression. (sciety.org)
The coordinated activities of promoter and enhancer regions in neural stem cells (NSCs) regulate gene expression programs that produce a vast diversity of neuronal and glial cell types. (indiana.edu)

Deletion of Jarid2 leads to impaired orchestration of gene expression during cell lineage commitment. (sdbonline.org)
Immune cell analysis revealed that the genes in the signature were correlated with B cell, CD4 T cell, CD8 T cell, dendritic cell, macrophage, and neutrophil. (hindawi.com)
Investigation of the regenerative process through live imaging and molecular studies revealed how this happens: The cardiomyocytes "dedifferentiate" - that is, they revert to an earlier form, something between an embryonic and an adult cell, which can then divide and differentiate into new heart cells. (eurekalert.org)
In the past, our research focused on viral genomes as models for DNA replication in mammalian cell nuclei. (nih.gov)
Promoters of genes associated with oxidative phosphorylation are preferentially remethylated at the 8-cell stage, suggesting that this mode of energy metabolism may not be favored. (nature.com)
Twenty years have passed since Dolly the sheep was born by cloning (somatic cell nuclear transfer, SCNT) but the results of non-human mammalian cloning are very poor, and cause animal diseases and huge biological losses. (sibi.org)
We demonstrate that neural stem/progenitor cell (NSPC) self-renewal and spatiotemporal generation of neurons and other cell types are severely impacted by the loss of Ythdf2 in embryonic neocortex. (biomedcentral.com)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
In addition to 5mC, the 6-methyladenine (6mA) DNA modification has recently been identified in eukaryotes, including mammalian stem cells, but its role in gene regulation and cell fate determination remains poorly understood. (hku.hk)
Because tissues are composed of several cell-types and even cells within the same cell-type have been shown to display dramatic cell-to-cell variability in gene expression, bulk measurements obscure quantification of how genetic or epigenetic features directly influence the function of individual cells. (confex.com)
Further, while cell-to-cell heterogeneity in gene expression has been shown to drive dramatic phenotypic variations, the upstream epigenetic mechanisms regulating this heterogeneity remain largely unknown 8 . (confex.com)
Role of mammalian Ecdysoneless in cell cycle regulation. (unmc.edu)
Our results demonstrate that mammalian Ecd plays a role in cell cycle progression via the Rb-E2F pathway. (unmc.edu)
However, the vast majority of biological knowledge has emerged as a consequence of studying cell populations and not individual cells. (justia.com)
For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
Thus, many of the basic functionalities for microfluidic single cell gene expression analysis have been demonstrated in isolation, including cell manipulation and trapping (9, 10), RNA purification and cDNA synthesis (11-13), and microfluidic qPCR (14) following off-chip cell isolation cDNA synthesis and preamplification. (justia.com)
Remarkably, our results show POLR2A and H3K36me3 can highly and consistently predict gene expression in three cell lines. (oncotarget.com)
Michael T. McManus focuses mostly in the field of Cell biology, narrowing it down to matters related to Embryonic stem cell and, in some cases, Chromatin. (research.com)
His scientific interests lie mostly in Cell biology, Gene, Computational biology, Cancer research and microRNA. (research.com)
His Gene study integrates concerns from other disciplines, such as Cell and Innate immune system. (research.com)
The ToxTracker assay is a panel of six validated GFP-based mouse embryonic stem (mES) reporter cell lines that can be used to identify the biological reactivity and potential carcinogenic properties of compounds in a single test. (europa.eu)
2011)*. To allow easy assessment of the activation status of these biomarker genes, green fluorescent (GFP) mES reporter cell lines were generated. (europa.eu)
Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)
11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)

His work has led to major advances in our understanding of mammalian cloning and embryonic stem cells. (wraltechwire.com)
We apply a tailored experimental design that eliminates these confounders, and report thousands of intrinsically covarying gene pairs in mouse embryonic stem cells. (nih.gov)
Our results lend support to the concept of post-transcriptional RNA operons, but we further present evidence that nuclear proximity of genes may provide substantial functional regulation in mammalian single cells. (nih.gov)
N6-Methyladenosine (m 6 A), a predominant internal modification of RNA in mammalian cells, has been recognized as having a vital role in mRNA stability, export, translation, splicing, and decay [ 4 ]. (hindawi.com)
This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
Examination of gene expression E-4031 dihydrochloride profiles has revealed dynamics between MSC, progenitor cells, and committed differentiated cells [29, 30]. (buyresearchchemicalss.net)
More recently, a unique set of microRNA have been shown to be associated with embryonic stem cells [43-48]. (buyresearchchemicalss.net)
Our current research now focuses on two basic, interrelated questions: (1) How do mammalian cells decide where and when to initiate DNA replication? (nih.gov)
Failure of mammalian cells to regulate their proliferation cycle leads to cancer. (nih.gov)
In fact, amplification of genes by over replication of certain regions of DNA is one of the primary mechanisms by which cancer cells become resistant to drug therapy. (nih.gov)
The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
As ChIP-seq cannot establish physical co-occurrence of two marks on the same allele, admixture of cells that either express (green) or do not express (red) the gene in focus could explain the occurrence of both marks as well as the low expression level in the overall population. (researchgate.net)
In contrast, in the case of ''true'' bivalency, virtually all cells in the population carry both marks simultaneously at the promoter in question, leading to low, if any, expression for that gene in all cells. (researchgate.net)
Many promoters in embryonic stem (ES) cells harbor a distinctive histone modification signature that combines the activating histone H3 Lys 4 trimethylation (H3K4me3) mark an. (researchgate.net)
Using mouse embryonic stem cells as a model system, our general experimental approach is to gain mechanistic insights by constructing both genetic interaction and protein-protein interaction maps of a key regulatory network. (northwestern.edu)
In 2016, the first discovery of 6mA in mammalian stem cells was reported, which sparked interest in elucidating its function. (hku.hk)
In this study, we used CRISPR-Cas9 gene editing to knock out the putative demethylase of 6mA DNA modification, ALKBH1, in human expanded potential stem cells (hEPSCs). (hku.hk)
Systematical profiling and comprehensive annotation of embryonic stem cells lincRNAs may not only bring a clearer big picture of these novel regulators but also shed light on their functionalities. (biomedcentral.com)
Single cells represent a fundamental biological unit. (justia.com)
Identification of binding sites of EVI1 in mammalian cells. (rochester.edu)
These reporters were created using bacterial artificial chromosomes (BAC) that contain the complete biomarker gene including promoter and regulatory elements ensuring physiological regulation of the GFP reporters following transfection into stem cells (Hendricks, G. et al. (europa.eu)
The remaining distal part of the IG-DMR was shown to bind pluripotency transcription factors in mouse embryonic stem cells (mESCs), exhibit active enhancer marks (H3K27ac), and nascent transcription 18 , 19 . (nature.com)
Recently, research has increased in the area of transplanting embryonic cells across species and growing kidneys and endocrine pancreas cells in situ. (medscape.com)

Finally, our mRNA-seq analyses revealed that IPMK and SMARCB1 regulate common gene sets, validating a functional link between IPMK and SWI/SNF complex. (sciety.org)

TFs can activate or block the initiation of gene transcription by binding to specific DNA sequences in enhancers or promoters [ 14 , 15 ] or recruiting some chromatin-modifying enzymes to induce the changes of chromatin structure [ 16 ]. (oncotarget.com)
20 ] noticed that different combinations of HMs are needed for predicting the expression levels of genes with different CpG content promoters. (oncotarget.com)

Hence, to estimate the effects of transcription factors binding and histone modifications on gene expression, we construct a statistical model for the genome-wide 15 transcription factors binding data, 10 histone modifications profiles and DNase-I hypersensitivity data in three mammalian. (oncotarget.com)
Moreover, genome-wide statistical redundancies exist within and between transcription factors and histone modifications, and these phenomena may be caused by the regulation mechanism. (oncotarget.com)

With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)

HO-1 and their products exert beneficial effects through the protection against oxidative injury, regulation of apoptosis, modulation of inflammation as well as contribution to angiogenesis. (springer.com)

The apparently scrambled linkage between ssTnI-cTnT and cTnI-ssTnT genes actually reflects original functional linkages as that TNNT2 gene is expressed together with ssTnI gene in embryonic cardiac muscle. (wikipedia.org)
Functional enrichment analysis suggested that these three genes may be involved in genetic and epigenetic events with known links to HCC. (hindawi.com)
The challenge with many wearable devices is that they don't have a communication interface that is functional on both the biological and electronic side to administer therapeutic loads effectively. (electropages.com)

New research at the Weizmann Institute of Science provides insight into the question of why the mammalian heart fails to regenerate, on one hand, and demonstrated, in adult mice, the possibility of turning back this fate. (eurekalert.org)
The team then created mice in which the gene for ERBB2 was knocked out only in cardiomyocytes. (eurekalert.org)
Mice carrying a hypomorphic Evi1 allele are embryonic viable but exhibit severe congenital heart defects. (rochester.edu)

His biological study spans a wide range of topics, including Knockout mouse and Argonaute. (research.com)

It has been suggested that over 30% of protein coding genes in humans are regulated by microRNA [42]. (buyresearchchemicalss.net)

To address this key issue for the five Xenopus orphan receptors previously identified, we first determined which could be transcriptionally activated by embryonic extracts and cocktails of candidate synthetic compounds. (uci.edu)

First, we are investigating how nucleosomes encode epigenetic information to influence gene expression using a chemical biology approach. (northwestern.edu)
1974. Biological handbooks: Biology data book. (cdc.gov)

I believe that the reprogramming errors are not the only cause of these low rates of cloning: the mammalian SCNT fails with a very high frequency mainly due to the damage that the technique itself inflicts in the egg and the somatic nucleus, and the very few successful cases occur only when the damage is not significant. (sibi.org)

We obtained the expression profiles of m 6 A-related genes for HCC patients from the International Cancer Genome Consortium and The Cancer Genome Atlas datasets. (hindawi.com)

This study systematically tested the function of all 13 Drosophila JmjC genes. (sdbonline.org)

Transcription factors and histone modifications are vital for the regulation of gene expression. (oncotarget.com)

Extensive whole-genome transcription profiling has led to identification of a panel of biomarker genes that are preferentially activated upon exposure to different classes of carcinogens and toxicants (Hendricks, G. et al. (europa.eu)

These results uncover a regulation loop where Jarid2 methylation fine-tunes PRC2 activity depending on the chromatin context. (sdbonline.org)
In this section, we comprehensively analyzed the expression levels of fourteen m 6 A RNA methylation regulators and clinical factors in patients with HCC from the ICGC (International Cancer Genome Consortium, https://icgc.org/ ), Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/ ), and TCGA (The Cancer Genome Atlas, http://cancergenome.nih.gov/ ) databases. (hindawi.com)

Various growth factors, cytokines, and prostaglandins upregulate HGF gene expression, including basic fibroblast growth factor, oncostatin M, hypoxia-inducible factor 1α and nuclear factor-κB (NF-κB) ( 9 ). (spandidos-publications.com)
Nrf2 stabilization and increase in its half-life even to 200 min [ 9 ] allows nuclear translocation and activation of transcription of cytoprotective genes (Fig. 1 ). (springer.com)

19 ] found that HMs or TFs binding in different positions show different predictive abilities, and they suggested HMs and TF binding may be redundant for predicting gene expression levels. (oncotarget.com)

Unlike the embryonic extract, the extracts of 84-h-old larvae produced only low-shifting binding complexes when incubated with a CPD-specific probe. (ncl.edu.tw)
One receptor, now termed BXR for benzoate X receptor, was activated by embryonic extracts. (uci.edu)
We employed a bioassay-directed fractionation of embryonic extracts and identified alkyl esters of amino and hydroxy benzoic acids potent stereoselective activators and endogenous BXR ligands. (uci.edu)

Our work indicates that parental origin of an epigenetic state is irrelevant as long as appropriate balanced gene expression is established and maintained at imprinted loci. (nature.com)

After implantation, a secondary DMR is established at the promoter of the Gtl2 gene, sustaining its repression from the paternal allele. (nature.com)

His microRNA research includes elements of Cancer research, Gene expression, Ribonuclease III, Regulation of gene expression and Gene silencing. (research.com)

Inositol polyphosphate multikinase (IPMK), a key enzyme in the inositol polyphosphate (IP) metabolism, is a pleiotropic signaling factor involved in major biological events including transcriptional control. (sciety.org)

In the embryonic brain, neurogenesis is accompanied by dynamic changes in chromatin accessibility and histone modifications within promoter and enhancer regions. (indiana.edu)

Canonically, neuroblastomas can be classified according to the MYCN (N-myc proto-oncogene protein) gene amplification, a common marker of tumor aggressiveness and poor prognosis. (bvsalud.org)
Assessment of F-MuLV-induced tumorigenesis reveals new candidate tumor genes including Pecam1, St7, and Prim2. (rochester.edu)

Each of the TnT isoform genes is linked in chromosomal DNA to a troponin I (TnI) isoform gene encoding the inhibitory subunit of the troponin complex to form three gene pairs: The fast skeletal muscle TnI (fsTnI)-fsTnT, slow skeletal muscle TnI (ssTnI)-cTnT, and cTnI-ssTnT pairs. (wikipedia.org)
Detailed analysis of the m 6 A-methylomes of Ythdf2 −/− NSPCs identifies that the JAK-STAT cascade inhibitory genes contribute to neuroprotection and neurite outgrowths show increased expression and m 6 A enrichment. (biomedcentral.com)

Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. (wikipedia.org)
We and others have synthesized light-activatable cMOs that allow spatiotemporal control of RNA splicing or translation, complementing the use of conditional knockouts to study stage- and tissue-specific differences in gene function. (cdc.gov)
18 , 19 We surmised that cyclic cMOs containing an NfsB-cleavable linker could be used with analogous lines to knock down gene function in a tissue-specific manner (Figure 1 a). (cdc.gov)

In yeasts, IPMK and its IP products were known to promote the activity of SWI/SNF chromatin remodeling complex, which plays a critical role in gene expression by regulating chromatin accessibility. (sciety.org)

These genetic circuits often come into play when medical conditions are being controlled, and many gene switches can be triggered in the presence of small molecular compounds such as antibiotics, vitamins, food additives, cosmetics, or volatile fragrances. (electropages.com)

Mammalian TNNT2 gene contains 14 constitutive exons and 3 alternatively spliced exons. (wikipedia.org)
Structure and evolution of the alternatively spliced fast troponin T isoform gene. (ncl.edu.tw)

This determined how hierarchical interactions between regulatory elements orchestrate robust parent-specific expression, with implications for non-imprinted gene regulation. (nature.com)

His studies deal with areas such as Endocrinology, Gene expression, Internal medicine, Messenger RNA and HOXB8 as well as Gene silencing. (research.com)

Anatomy29

Organisms26

Diseases1

Chemicals and Drugs59

Analytical, Diagnostic and Therapeutic Techniques and Equipment43

Phenomena and Processes84

Disciplines and Occupations7

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science11

Health Care7

Epigenetic regulation6

Stem cell differenti2

Developmental7

Pathways7

Expression of ge3

Target genes3

Regulates4

Phenotypes5

Transgenic3

Genomic6

Early embryonic1

Late embryonic1

Human embryonic7

Transcriptional regulation5

Cellular8

Genetics5

RNAs1

Processes6

Embryogenesis2

Differentiation4

Development14

Proteins4

Pluripotent4

Regulate gene expression3

Cell26

Cells23

MRNA1

Promoters2

Genome-wide2

Genomes1

Apoptosis1

Functional3

Mice3

Mouse1

Protein codin1

Transcriptionally1

Biology2

Somatic1

Datasets1

Drosophila1

Transcription factors1

Preferentially1

Methylation2

Nuclear2

Predicting gene expression1

Extracts3

Loci1

Allele1

MicroRNA1

Metabolism1

Histone modifications1

Tumor2

Inhibitory2

Tissue-specific3

Role in gene1

Molecular1

Alternatively spliced2

Regulatory1

Silencing1