Regulation of epithelial cell cell junctions cell cycle. Medical search. Frequent questions

The present work introduces a computational implementation of some principles of a theory of organism development, namely that the default state of cells is proliferation and motility, and includes the principle of variation and organization by closure of constraints. (nature.com)
In the present model, the bioelectric context of cells and tissue is the field responsible for organization, as it regulates cell proliferation and the level of communication driving the system's evolution. (nature.com)
Starting from a depolarized (proliferative) cell, the organism grows to a certain size, limited by the increasingly polarized state after successive proliferation events. (nature.com)
Previous researches completed by our team have also demonstrated that resveratrol inhibits the proliferation, invasion, and metastasis of colorectal cancer cells [ 5 ]. (hindawi.com)
The objective of this study was to investigate the effect of dietary restriction and subsequent compensatory growth on the relative expression of genes involved in volatile fatty acid transport, metabolism and cell proliferation in ruminal epithelial tissue of beef cattle. (biomedcentral.com)
We hypothesized that in animals undergoing compensatory growth there would be an up-regulation of the relative expression of genes involved in VFA absorption and metabolism, as well as genes underlying growth and proliferation of the ruminal epithelium. (biomedcentral.com)
Microarray analysis reveals that inhibition of Notch signalling causes downregulation of stem cell-associated genes in parallel with decreased proliferation and increased numbers of ciliated cells and that organoids also respond to oestradiol and progesterone treatment in a physiological manner. (nature.com)
Recently, signaling by GCC and its downstream effector, cyclic GMP (cGMP) has emerged as a principal regulator of proliferation in human colon cancer cells. (jefferson.edu)
In close agreement, induction of GCC signaling in mucosal sheets ex vivo and intestinal cells in vitro inhibited proliferation by activating cGMP-dependent protein kinase and delaying the cell cycle at the G1/S transition. (jefferson.edu)
Separately, deletion of GCC increased tumor growth by releasing a restriction on the cell cycle normally constraining epithelial cell proliferation. (jefferson.edu)
Even though its sequence along with its expressional pattern in cancer cell lines are evident, the functional aspects concerning cell proliferation, viability, differentiation and cell cycle regulation of NDRG1 remains vague. (scirp.org)
Unpredictably, cell cycle progression, cell proliferation and DNA synthesis were unaffected fol lowing NDRG1 expression. (scirp.org)
T. Napso, N. Azzam, A. Lerner and F. Fares, " N-myc Downstream Regulated Gene 1 Increases Differentiation Factors Level in Human Prostate Cancer Cells without Affecting Cell Proliferation and Cell Cycle Profiles," Journal of Cancer Therapy , Vol. 4 No. 2, 2013, pp. 568-574. (scirp.org)
Measured by its ability to block anti-ErbB2 mediated inhibition of SK‑BR‑3 human breast cancer cell proliferation. (novusbio.com)
Uncontrolled cell proliferation leads to increased risk of genetic instability. (ghise-ioan.ro)

The tumor suppressor gene LKB1, also known as serine/threonine protein kinase 11 (STK11 ), encodes a serine/threonine protein kinase that has multiple cellular functions, including tumor suppression, cell cycle regulation, and promotion of apoptosis. (biomedcentral.com)
Crypt hyperplasia in GCC-/- mice was associated with compensatory increases in cell migration and apoptosis. (jefferson.edu)
Previous studies indicated that endotoxins usually caused intestinal mucosa inflammation and destruction of tight junction integrity and epithelial cell apoptosis [ 4 ], which subsequently led to disruption of intestinal homeostasis and damage of the intestinal barrier functions. (biomedcentral.com)
GSEA showed that high-risk patients were highly associated with apoptosis, cell cycle, ribosome, base excision repair, aminoacyl-tRNA biosynthesis, and mismatch repair. (biomedcentral.com)
Moreover, reduction of the effectiveness of the mucosal barrier due to decreased secretion of antimicrobial molecules and mucins and premature apoptosis of stressed cells could exacerbate the inflammatory environment typical of IBD. (biomedcentral.com)
Interacting with various low risk hpv cause cancer proteins, E6 and E7 influence fundamental cellular functions like cell cycle regulation, telomere maintenance, susceptibility to apoptosis, intercellular adhesion and regulation of immune responses. (ghise-ioan.ro)
In a different study, in which researchers systematically analyzed mutations identical to those found in patients with Darier disease, mutant SERCA2 protein aggregates were found to cause stress to the endoplasmic reticulum, subsequently inducing cell apoptosis. (medscape.com)
This phase involves the restitution of cell polarity and tight junction integrity in sublethally injured cells, removal of dead cells by apoptosis, removal of intratubular casts by reestablishment of tubular fluid flow, and regeneration of lost renal epithelial cells. (medscape.com)

The presence of 'stem cell-like' cells has previously been postulated based on sphere-forming capacity and differentiation in vitro 1 and evidence for the presence of label-retaining cells in the distal fallopian tube 2 . (nature.com)
Our data reveal a significant overlap between Notch-dependent genes in the fallopian epithelium and the defined 'stem cell signature' of the mouse intestine, suggesting the existence of a conserved pathway that regulates tissue renewal and directly controls cell fate specification and differentiation in the organoid by inhibiting cilliogenesis. (nature.com)
These results revealed that NDRG1 is functional in prostate cancer cells and able to induce expression of differentiation factors through p53 independent pathway. (scirp.org)
A Novel Gene Which Is Up-Regulated during Colon Epithelial Cell Differentiation and Down-Regulated in Colorectal Neoplasms," Laboratory Investigation: A Journal of Technical Methods and Pathology, Vol. 77, No. 1, 1997, pp. 85-92. (scirp.org)
This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. (cancer-genetics.org)

Studies in epithelial cells have indicated that Popdc1 plays a role in cell-cell conversation and adhesion and that the conversation of Popdc1 molecules with one another is important for the maintenance of intercellular junctions [8]C[10]. (sdfca.org)
Electron microscopy reveals loss of desmosomes (epithelial intercellular junctions formed by membrane and submembrane protein complexes), breakdown of desmosome-keratin intermediate filament attachment, and perinuclear aggregates of keratin intermediate filaments. (medscape.com)

Broadly separated into mesenchymal and endothelial cells, NES have been implicated in thymus organogenesis, thymocyte development, tolerance induction and development/maintenance of epithelial stroma. (frontiersin.org)

We measured the effects of cytokine exposure of CF and non-CF well-differentiated main human being airway epithelial cells on TJ properties including transepithelial resistance paracellular permeability to hydrophilic solutes and the TJ proteins occludin claudin-1 claudin-4 junctional adhesion molecule and ZO-1. (scienceexhibitions.org)
Here, we applied a liquid chromatography-mass spectrometry (LC-MS/MS) approach to identify proteins modulated after beta-catenin knockdown in the breast cancer cell line MCF-7. (unich.it)
We used a label free analysis to compare trypsin-digested proteins from CTR (shCTR) and beta-catenin knockout cells (sh beta cat). (unich.it)
In detail, proteins involved in carbohydrate metabolism and tricarboxylic acid cycle were found to be down-regulated, whereas proteins associated to lipid metabolism were found up-regulated in sh beta cat compared to shCTR. (unich.it)
In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. (thermofisher.com)
In eukaryotic cells, chromatin comprises a complex consisting of DNA, RNA, and proteins where covalent modifications correlate with regulation of gene expression [ 1 ]. (biomedcentral.com)
Dr. Amitabha Mukhopadhyay, National Institute of Immunology, New Delhi- Regulation of Rab proteins by cytokines. (jnu.ac.in)
Some studies of keratosis follicularis (Darier disease) have suggested that alterations in calcium regulation may affect the synthesis, folding, or trafficking of desmosomal proteins. (medscape.com)
The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)

Significantly, there are additional non-epithelial stromal cells (NES) that exist alongside TEC within thymic microenvironments, including multiple subsets of mesenchymal and endothelial cells. (frontiersin.org)
In early experiments, physical separation of mesenchymal and epithelial stroma from embryonic murine thymus resulted in defective thymus development when epithelium was cultured in vitro in the absence of mesenchyme ( 7 ). (frontiersin.org)
However, little work has been done on the roles of LKB1 in cell polarity and epithelial-mesenchymal transition in breast cancer. (biomedcentral.com)
The roles of LKB1 in cell polarity and epithelial-mesenchymal transition in breast cancer were determined by using immunofluorescence, western blot assay, and cell migration and invasive assays. (biomedcentral.com)
In vitro analysis revealed that loss of LKB1 expression enhanced migration, invasion and the acquisition of mesenchymal phenotype, while LKB1 overexpression in MDA-MB-435 s cells, which have a low basal level of LKB1 expression, promoted the acquisition of epithelial phenotype. (biomedcentral.com)
beta-catenin plays an important role as regulatory hub in several cellular processes including cell adhesion, metabolism, and epithelial mesenchymal transition. (unich.it)
In the present study we examined the protective potential of exogenously administered human umbilical cord derived mesenchymal stem cells (UCMSCs) against Dextran Sulfate Sodium (DSS) induced acute colitis in immunodeficient NOD.CB 17 - Prkdc scid /J mice with particular attention to endoplasmic reticulum (ER) stress. (biomedcentral.com)

This study explores the use of a simple human neurosphere-based in vitro model to characterise the pharmacological and toxicological effects of LiCl and VPA using gene expression changes linked to phenotypic alterations in cells. (plos.org)
Human embryonic stem cells (hESc) can also be used to analyze the in vitro development from undifferentiated pluripotent cells leading to terminally differentiated cell types, recapitulating the process of early embryonic development [5] . (plos.org)
Skeletal muscle and myogenic C2C12 cells were used to study the effects of MSY3 phosphorylation in vivo and in vitro on its sub-cellular localization and activity, by blocking the IGF1/PI3K/Akt pathway, by Akt depletion and over-expression, and by mutating potential MSY3 phosphorylation sites. (biomedcentral.com)
We show that single epithelial stem cells in vitro can give rise to differentiated organoids containing ciliated and secretory cells. (nature.com)
Here we demonstrate the existence of adult stem cells in the human fallopian tube epithelium, which gives rise to a monolayer of differentiated epithelial cells in a complex 3D organoid in vitro . (nature.com)
Furthermore, POGLUT2 knockdown of breast cancer cells was established, followed by in vitro biological function assays and in vivo tumor growth study. (frontiersin.org)

But these treatments, to some extent, easily produce side effects on normal cells, organs, and other tissues of the human body, thus accelerating the death process of cancer patients. (hindawi.com)
While many studies have demonstrated the importance of cTEC and mTEC during T-cell development, thymic stromal microenvironments also contain heterogenous non-epithelial stromal (NES) populations in a similar manner to that seen in peripheral lymphoid tissues. (frontiersin.org)
Alterations of bioelectrical properties of cells and tissues are known to function as wide-ranging signals during development, regeneration and wound-healing in several species. (biomedcentral.com)
Increased levels of POGLUT2 were found in multiple types of cancer tissues and cell lines. (frontiersin.org)

This is mainly achieved by its dual role as structural component of cadherin-based adherens junctions, and as a key nuclear effector of the Wnt pathway. (unich.it)

Epithelial restricted junctions (TJs) provide an important route for passive electrolyte transport across airway epithelium and provide a barrier to the migration of harmful materials from your lumen to the interstitium. (scienceexhibitions.org)
One group of disorders inflammatory bowel disease displays a highly deregulated barrier function that appears to be a fundamental event in disease AG-1024 pathogenesis (Schmitz DNA polymerase for 25 cycles. (scienceexhibitions.org)
The first evidence of amoebic pathology is local depletion of intestinal mucus and disruption of the epithelial barrier as a result of degradation of the extracellular matrix, which occurs in part from the action of cysteine proteases. (blastocystis.net)
The epithelial cells of the gastrointestinal tract, because of their barrier function, are exposed to toxins and infectious agents that can adversely affect protein folding in the ER and cause ER stress. (biomedcentral.com)
This layer ranges in thickness from 15-100 or more cells depending on anatomic location and is the primary protective barrier from the external environment. (medscape.com)

Expression of DGL7 is found in cancer and stem cells. (wikipedia.org)
Stem Cells. (wikipedia.org)
Whilst LiCl exposure did not significantly alter the proportion of cells expressing markers for stem cells/undifferentiated cells (Oct4, SSEA4), neurons (Neurofilament M), astrocytes (GFAP) or cell cycle phase, the drug caused a 1.4-fold increase in total cell number. (plos.org)
Of particular interest is their contribution to Developmental Neurotoxicity (DNT) as well as their potential impact on stem cells. (plos.org)
Here we report on the establishment of long-term, stable 3D organoid cultures from human fallopian tubes, indicative of the presence of adult stem cells. (nature.com)

These microenvironments are classically defined as distinct cortex and medulla regions that each contain specialized subsets of stromal cells. (frontiersin.org)
While adult lymphoid progenitor cells are produced by the bone marrow, their lineage commitment and development into mature T-cells is dependent on their migration into the thymus, where essential interactions with heterogeneous thymic stromal cells take place ( 1 ). (frontiersin.org)
Arguably, the most recognized and well-studied stromal populations in thymus biology are thymic epithelial cells (TEC) within the cortical and medullary areas, which are defined by their anatomical separation and function. (frontiersin.org)
POGLUT2 was mainly expressed in stromal cells as verified by StromalScore, ESTIMATEScore, ImmuneScore, and Tumor purity, and POGLUT2 was positively correlated with cancer-associated fibroblasts, macrophages, monocytes, and neutrophils in the tumor microenvironment. (frontiersin.org)
Finally, in situ hybridization analyses revealed the presence of RP11-295G20.2 in the nuclei of corneal epithelial cells, but not in the stromal cells of the human cornea, while RP11-322M19.1 was present in epithelial and non-epithelial cells. (molvis.org)

These results provide an insight into the potential molecular mechanisms regulating ruminal epithelial absorptive metabolism and growth following nutrient restriction and subsequent compensatory growth. (biomedcentral.com)
Thus, LKB1 regulates multiple biological pathways involved in cell growth and metabolism. (biomedcentral.com)
β-Catenin Knockdown Affects Mitochondrial Biogenesis and Lipid Metabolism in Breast Cancer Cells. (unich.it)
Our data highlight a role of beta-catenin in the regulation of metabolism and energy homeostasis in breast cancer cells. (unich.it)
Additionally, a viable therapeutic strategy may involve triggering the cell death mechanism in cancer cells by blocking mitochondrial metabolism with a natural substance. (scientificarchives.com)
It is involved in the regulation of carbohydrate and lipid metabolism, as well as the functioning of the reproductive, cardiovascular, and nervous system ( 1 ). (spandidos-publications.com)

Enzyme-linked Immunosorbent Assay Primary HAE cells were treated with cytokines for 24 48 or 72 h and total protein was isolated as described above. (scienceexhibitions.org)
ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. (thermofisher.com)
We aim to study the coupling between LTCC and β adrenergic receptors in different cardiomyocyte microdomains, the distinct involvement of PKA and CAMKII (Ca2+/calmodulin-dependent protein kinase II) and explore how this functional complex is disrupted in heart failure.Methods:Global signaling between LTCCs and β adrenergic receptors was assessed with whole-cell current recordings and western blot analysis. (imperial.ac.uk)
Interestingly, BMGlvA2 improved the distribution and abundance of tight-junction protein ZO1 in duodenum and ileum epithelium after ETEC-challenge. (biomedcentral.com)
ER stress induces activation of the unfolded protein response (UPR), a signaling network that is required to resolve stress, restoring ER homeostasis and promoting cell survival and adaptation. (biomedcentral.com)
The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. (cancer-genetics.org)
The investigators found that the resultant transfected cells showed defects in ATP2A2 protein expression (15 mutants), ATP hydrolysis (29 mutants), calcium transport (4 mutants), and calcium binding and kinetics (3 mutants). (medscape.com)

In that context, differentiated enterocytes in villi exhibit higher guanylin expression and ligand-dependent cGMP accumulation compared to proliferating progenitor cells in crypts. (jefferson.edu)
Crypt expansion reflected hyperplasia of the proliferating compartment with reciprocal increases in rapidly cycling progenitor cells and reductions in differentiated cells of the secretory lineage, including Paneth and goblet, but not enteroendocrine, cells. (jefferson.edu)

Tyrosine kinase inhibitors (TKIs) against the human epidermal growth factor receptor (EGFR) are now standard treatment in the clinic for patients with advanced EGFR mutant non-small-cell lung cancer (NSCLC). (researchgate.net)
The epithelial lining of the fallopian tube is of critical importance for human reproduction and has been implicated as a site of origin of high-grade serous ovarian cancer. (nature.com)
The relationships of LKB1 expression to clinic-pathological parameters and epithelial markers E-cadherin and high-molecular-weight -cytokeratin (HMW-CK) were investigated in 80 clinical breast cancer tissue samples and their paired normal control breast tissue samples by using immunohistochemistry. (biomedcentral.com)
Then, the LKB1 expressions in metastatic and non-metastatic breast cancer cell lines were compared. (biomedcentral.com)
Knockdown of endogenous LKB1 gave rise to dysregulation of cell polarity and invasive phenotype of breast cancer cells. (biomedcentral.com)
NAR can be encapsulated by chitosan nanoparticles-TPP conjugates, thereby improving NAR cellular absorption and cytotoxicity against cancer cells. (scientificarchives.com)
Evidence indicating that OXPHOS plays a substantial role in the development of many cancer cells has led to the growing recognition of cancer as a metabolic disease. (scientificarchives.com)
We also present a thorough analysis of the role of succinate in the initiation of metabolic and epigenetic changes that contribute to the development of cancer, as well as its effects on angiogenesis, cell invasion, and migration. (scientificarchives.com)
Furthermore, human cDNA of NDRG1 from normal placenta was cloned into a eukaryotic expression vector and transfected into the three cancer cell lines. (scirp.org)
Loss of sensitivity to p21 cell cycle control may be associated with prostatic cancer behavior. (scirp.org)
Senescent cells have been identified in the aging prostate, and the senescence-associated secretory phenotype might be linked to prostate cancer (PCa). (biomedcentral.com)
Members of this family of receptors are involved in regulation of numerous physiological and patho-physiological processes and have great potential as targets for the treatment of diseases such as cancer, diabetes, coronary heart disease and asthma. (jnu.ac.in)
Emerging techniques using the analysis of circulating tumor cells show promising results in predicting and identifying the early stages of breast cancer metastasis in patients. (iiarjournals.org)
This method uses either epithelial cell-specific markers which are generally expressed in all tumor cells, or tumor markers expressed by specific types of cancer ( 13 ). (iiarjournals.org)
Expression, gene mutation and amplification, methylation, and copy number alteration (CNA) of POGLUT2 were evaluated using The Cancer Genome Atlas (TCGA), Cancer Cell Line Encyclopedia (CCLE), and Genotype-Tissue Expression (GTEx) databases. (frontiersin.org)
Increased levels of POGLUT2 could result in the dysregulated immune cell infiltration and tumor microenvironment and showed a significant regulatory effect on the progression of breast cancer through Notch-related signaling. (frontiersin.org)
Schematic low risk hpv cause cancer of the HPV double-stranded circular DNA genome Journal of Virology Nov HPV integration into the host genome and Papillomavirus life low risk hpv cause cancer To establish infection, the virus must infect basal epithelial cells of stratified squamous epithelium, that are long lived or have stem cell-like properties. (ghise-ioan.ro)
Microtrauma of the suprabasal epidermal cells enables the virus to infect the cell within the low risk hpv cause cancer layer. (ghise-ioan.ro)
Role of Dusp6 Phosphatase as a Tumor Suppressor in Non-Small Cell Lung Cancer. (cancer-genetics.org)
In the USA, obesity has recent- obesity is associated with progres- aberrations of cancer cells, summa- ly surpassed tobacco use as the sion but not incidence [9]. (who.int)

The system reaches homeostasis, with a depolarized core (proliferative cells) surrounded by a rim of polarized cells (non-proliferative in this condition). (nature.com)
This theory would deal with the way an organism spontaneously grows from a single cell to a complete and complex organism, introducing organization at the different development stages and homeostasis for a mature organism. (nature.com)
The significance of loss of GCC ligands early in intestinal tumorigenesis remains obscure because (patho)physiological roles of GCC signaling in intestine, beyond regulation of intestinal fluid and electrolyte homeostasis, are poorly defined. (jefferson.edu)
Defective regulation of iron homeostasis genes lead to either, iron excess and related tissue injuries due to iron-stimulated oxidative damage or iron deficiency disorders. (jnu.ac.in)

In C2C12 myogenic cells, blocking the IGF1/PI3K/Akt pathway using LY294002 inhibitor reduced MSY3 phosphorylation levels resulting in its accumulation in the nuclei. (biomedcentral.com)
This study highlights a previously undescribed Akt-mediated signaling pathway involved in the repression of myogenin expression in myogenic cells and in mature muscle. (biomedcentral.com)
By using blockers of PKA and CaMKII and a Caveolin-3-knockout mouse model, we conclude that the β2AR-LTCC regulation requires the presence of caveolin-3 and the activation of the CaMKII pathway. (imperial.ac.uk)
However, the pathway downstream NDRG1, involving p21 and c8/18, regulating cell cycle progression and DNA synthesis is unfunctional. (scirp.org)
In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)

Real-time qPCR was used to quantify mRNA transcripts of 26 genes associated with ruminal epithelial function. (biomedcentral.com)
Furthermore, Notch is responsible for the regulation of known stem cell factor genes. (nature.com)
K. Kokame, H. Kato and T. Miyata, "Homocysteine-Respondent Genes in Vascular Endothelial Cells Identified by Differential Display Analysis. (scirp.org)
The viral genome maintains itself as an episome in basal cells, where the viral genes are poorly expressed. (ghise-ioan.ro)

Using the early embryonic divisions of the ascidian Phallusia mammillata as a model to investigate mechanisms of unequal cell division, this study convincingly demonstrates that cell shape and cortical domains are cooperating, rather than competing, in order to establish cell size asymmetry, a significant conceptual advance for the field. (elifesciences.org)
Western blot analysis of HEK293 embryonic kidney cell line whole cell lysate probed with Mouse anti Human c-myc antibody ( MCA2200 ) followed by HRP conjugated Goat anti Mouse IgG secondary antibody visualized using chemiluminescence. (bio-rad-antibodies.com)

The Drosophila follicle-cell epithelium provides an appropriate model system for studying the potential role of electrochemical signals, like intracellular pH (pH i ) and membrane potential (V mem ), during development. (biomedcentral.com)
Our data show that in the Drosophila follicle-cell epithelium stage-specific pH i - and V mem -gradients develop which result from the activity of several ion-transport mechanisms. (biomedcentral.com)

In this study, we tried to prove that loss of LKB1 disrupts breast epithelial cell polarity and causes tumor metastasis and invasion. (biomedcentral.com)
An emerging method to detect metastasis is the analysis of circulating tumor cells (CTCs), which has shown promise in filling the gaps left by other diagnostic methods. (iiarjournals.org)

ZO-1 may be involved in signal transduction at cell-cell junctions. (thermofisher.com)

The SCFAs are rapidly adsorbed from the colonic lumen and constitute a preferred energy source for colonic epithelial cells. (biomedcentral.com)

Possible MSY3 mechanisms of action have been studied in cell culture studies. (biomedcentral.com)
By contrast, at a cellular "global" level PKA plays a major role downstream β1AR and results in an increase in LTCC current.Conclusions:Regulation of the LTCC activity by proximity coupling mechanisms occurs only via β2AR, but not β1AR. (imperial.ac.uk)

Cortical epithelial cells (cTEC) support the earliest thymocyte progenitor populations, guiding them through the cortex, directing them to a T-cell lineage fate and ensuring their functional qualities as self-MHC restricted cells through the process of positive selection ( 2 ). (frontiersin.org)
We found that the UCDs and their orientation at the ascidian third cleavage rely on the spindle tilting in an anisotropic cell shape, and cortical polarity domains exerting different effects on spindle astral microtubules. (elifesciences.org)
We therefore propose that the spindle position during UCD is set by the combined activities of cell geometry and polarity domains, where cell geometry modulates the effect of cortical polarity domain(s). (elifesciences.org)

The production of a diverse αβ-T-cell pool is vital to establishing and maintaining a functional adaptive immune system. (frontiersin.org)
Clinical correlation, external validation, functional enrichment analysis, drug and cell line analysis, and tumor immune environment analysis were conducted. (biomedcentral.com)
Inflammation can be induced directly by UPR pathways in stressed cells, or indirectly through interaction with immune cells recruited by inflammatory cytokines released from stressed cells [ 11 ]. (biomedcentral.com)

Involvement of Popdc1 in the regulation and signaling of tight junction formation and function, vesicular transport and receptor cycling has been exhibited [11]C[14]. (sdfca.org)
INTRODUCTION Tight junctions (TJs) are characteristically located at the apicolateral borders of adjacent epithelial cells and are responsible for the selective regulation AG-1024 of the passage of ions and neutral molecules through the paracellular space. (scienceexhibitions.org)
ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)

However, potential effects of compensatory growth following dietary restriction and subsequent re-alimentation, on ruminal epithelial function have not been assessed, to date. (biomedcentral.com)
Ki-67-positive cells were distributed along the epithelial folds, without restriction to a particular region with occasional clustering ( Fig. 1a , asterisk). (nature.com)

Dr. Mukhopadhyay did his doctoral studies in Biochemistry at the Department of Biochemistry from Calcutta University and received the postdoctoral training in the Department of Cell Biology, Cleveland Clinic Foundation, USA. (jnu.ac.in)

J. Cell Biol. (wikipedia.org)

Permeability was assessed by the efflux of fluorescein isothiocyanate (FITC)-dextran permeated through the monolayer endothelial cells (ECs). (springer.com)

Cav3 appears in the cardiomyocyte sarcolemma, intercalated discs and T-tubules and was recognized in the costameres, rib-like perisarcolemmal multiprotein complexes that align with Z disks and T-tubules and function in cell adhesion, stretch-sensing and pressure transmission [28], [29]. (sdfca.org)

Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)

It is lined by simple columnar epithelium containing secretory and ciliated cells, which produce tubular fluid and facilitate transport of gametes, respectively. (nature.com)

Carcinogenesis is introduced through a localized event (a spot of depolarized cells) or by random depolarization of cells in the tissue, which returns cells to their initial proliferative state. (nature.com)
This simplified model of embryogenesis, tissue organization and carcinogenesis, based on non-excitable cells' bioelectric properties, can be made more realistic with the introduction of other components, like biochemical fields and mechanical interactions, which are fundamental for a more faithful representation of reality. (nature.com)

Indeed, both the hESc and human neuroprogenitor cell (hNPC) models provide valid and useful tools for studying DNT. (plos.org)
Finally, the non-transformed human breast cell line MCF-10A was cultured in three dimensions to further reveal the role of LKB1 in breast epithelial cell polarity maintenance. (biomedcentral.com)
Alexa-FluorA647 conjugated Mouse anti Human c-myc antibody, clone 9E10 ( MCA2200A647 ) used for the evaluation of c-myc on dicidual NK cells demonstrating the correlation between c-myc and CD122 expression. (bio-rad-antibodies.com)
Das D, Tapryal N, Goswami S.K., Fox P.L, and Mukhopadhyay C.K. Regulation of Ceruloplasmin in human hepatic cells by redox active copper: Identification of a novel AP-1 site in ceruloplasmin gene. (jnu.ac.in)
Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)

A loss of mitochondrial mass and membrane potential was also assessed by fluorescent probes in sh beta cat cells with respect to the controls. (unich.it)
This single cell layer of keratinocytes is attached to the basement membrane via hemidesmosomes. (medscape.com)

LKB1 also plays crucial roles in establishment and maintenance of cell polarity. (biomedcentral.com)

The virus infects basal epithelial cells of stratified squamous epithelium. (ghise-ioan.ro)
Once inside the host cell, HPV DNA replicates as the basal cells differentiate and progress to the surface of the epithelium. (ghise-ioan.ro)

In this review, we cover how the non-epithelial compartment of thymic stroma represent essential cell populations in regulating thymus function. (frontiersin.org)

Extensive research on thymic epithelial cells (TEC) within the cortex and medulla has defined their essential roles during T-cell development. (frontiersin.org)
Their findings provide a new perspective on the roles of cell polarity and shape in the control of spindle positioning, and are of broad interest to cell and developmental biologists. (elifesciences.org)

We have previously shown that MSY3/Csda binds a highly conserved cis-acting element located upstream of the myogenin promoter (myogHCE) and regulates the postnatal down-regulation of myogenin [ 16 ]. (biomedcentral.com)

Compared to controls, beta-catenin knockout cells showed increased incorporation of [1-C-14] acetate and decreased utilization of [U-C-14] glucose for fatty acid synthesis. (unich.it)

Clearance of small intestinal crypts involves goblet cell mucus secretion by intracellular granule rupture and enterocyte ion transport. (gu.se)

The stratum germinativum, or the basal layer, is immediately superficial to the dermoepidermal junction. (medscape.com)

To further investigate PNN's role in regulating the alternative splicing of lncRNAs in a corneal epithelial context, we performed detailed analyses for detecting and identifying alternatively spliced lncRNAs. (molvis.org)

[ 22 ] Investigators transfected a fibroblast cell line with 51 different mutations seen in keratosis follicularis (Darier disease) pedigrees. (medscape.com)

Dr. Paul Fox, Cleveland Clinic Foundation, USA- Posttranscriptional Gene Regulation. (jnu.ac.in)

Finally, it was found for the first time that endogenous LKB1 knockdown resulted in abnormal cell polarity in acini formed by non-transformed breast epithelial cells grown in 3D culture. (biomedcentral.com)
Knockdown of PNN in HCET cells led to specific changes in the inclusion of multiple cassette exons as well as in the use of alternative splice sites in RP11-322M19.1 and RP11-18I14.1, resulting in considerable net changes in the ratio between the splice variants. (molvis.org)

James Miller introduced the living systems theory 4 , where each system must include a number of critical subsystems, dealing with their structure, interaction, behavior and development, that can be applied from simple cells to full organisms. (nature.com)
The thymus supports T-cell development via specialized microenvironments that ensure a diverse, functional and self-tolerant T-cell population. (frontiersin.org)
However, experimental evidence suggests these cells are important for thymus function by either directly influencing T-cell development, or by indirectly regulating TEC development and/or function. (frontiersin.org)
In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)
During the fourth week of embryologic development, the single cell thick ectoderm and underlying mesoderm begin to proliferate and differentiate. (medscape.com)

These gradients are supposed to represent important bioelectrical cues during oogenesis, e.g., by serving as electrochemical prepatterns in modifying cell polarity and cytoskeletal organisation. (biomedcentral.com)
LKB1, also known as STK11 , is a master kinase that serves as an energy metabolic sensor and is involved in cell polarity regulation. (biomedcentral.com)
Furthermore, LKB1 helps establish and maintain cell polarity in mammals. (biomedcentral.com)
Cell division orientation is thought to result from a competition between cell geometry and polarity domains controlling the position of the mitotic spindle during mitosis. (elifesciences.org)
Depending on the level of cell shape anisotropy or the strength of the polarity domain, one dominates the other and determines the orientation of the spindle. (elifesciences.org)
Here, we show that cell geometry and polarity domains cooperate, rather than compete, in positioning the cleavage plane during UCDs in early ascidian embryos. (elifesciences.org)
By systematically varying mitotic cell shape, we could modulate the effect of attractive and repulsive polarity domains and consequently generate predicted daughter cell size asymmetries and position. (elifesciences.org)
Yet, whether and how cell geometry and polarity domains compete with each other not only to determine the orientation but also the centering of the mitotic spindle leading to equal or unequal cell divisions (UCDs) remains unclear. (elifesciences.org)

Zinc possesses anti‑inflammatory activity by inhibiting NF‑κB signaling and modulation of regulatory T‑cell functions that may limit the cytokine storm in COVID‑19. (spandidos-publications.com)

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